LOC105221400 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105221400
Entrez Gene ID	105221400
Full Name	WD repeat domain 35
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011198362.2	XP_011196664.1	WD repeat-containing protein 35
XM_011198362.1	XP_011196664.1	WD repeat-containing protein 35

Pathways from BioSystems
Gene Ontology

The following LOC105221400 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105221400 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa193683	XM_011198362.2 Latest version!	Zeugodacus cucurbitae WD repeat-containing protein 35 (LOC105221400), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBa193683	XM_011198362.1	Bactrocera cucurbitae WD repeat-containing protein 35 (LOC105221400), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105221400 ( XM_011198362.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196664.1 Zeugodacus cucurbitae WD repeat-containing protein 35 (LOC105221400), mRNA.

Price & Availability↑

CloneID	OBa193683
Clone ID Related Accession (Same CDS sequence)	XM_011198362.2 , XM_011198362.1
Accession Version	XM_011198362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	WD repeat-containing protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011198362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAT ATTTAAGTAA AAAGATTGCC ATACCAAACA ATGTAAAATT GAATTGCATA 
GCTTGGAATA AGGAGCATGG GTACATAGCT GTCGCTGGTT CTGAGGGCCT ACTTAAGGTG 
TTGAAACTAG AGCAAGCATC CAGTGGGCCA AATAAGGGGA ATCTAGCTGC CGTATCAAAC 
TTGTCCATGA ATCAAACACT GGACGGGCAT AAGGAAGGGG TTAAAGTGGT CACTTGGAAT 
GAGACGCAGC AGAAATTGAC TTCTTCGGAT CTGGATGGAG TTATTATGGT TTGGATGATC 
TATAAAGGTT CTTGGTACGA AGAGATGACT AATGATCGCA AAAAATCCAC CGTTAATAGT 
ATGAGTTGGA CATCTGATGG TGCCAAAATA TGCATAGTGT ATGAAGATGG GGCCATTATT 
GTAGGCTCAG TGGATGGAAA TCGTATATGG GGCAAGGAAC TAAAGAATAC ACATTTAACT 
AGTGTGCAAT GGAGTCCGGA TAATCGCTTG ATTTTATTTG GTTTAGCTAA TGGTGAATGT 
CATCTATATG ACAACCAAGG AAATTTTGCG ATGAAGCTAA ATATACAATG CGTCTCGCTC 
AGTCCAAATA GAAACTTTAG AATTTCGGCA CTTTGTTGGT ACAGCGGAAG AGCACCATCC 
AACAGACCAG TTCTAGCGAT TTGTTTTGAG ACGTGCAAAT TGCAAATTAT GCGTAATGAA 
ATCGATGACG CCCCAGTTAT CATCGATACT GGCATGCGAA ATATAGACGC GGAGTGGAAT 
CAAGACGGTT CTGTACTATC TGTTTGTGGA ACTCTTATAG ATTACAGTAC TTCGAAGGAA 
TCAAATCAAG TAAACTTTTA CTCGCCATTC GGACAGTTGA TACGCATTCT GAAGGTTCCA 
GGAAATGAGA TCTCTTCCCT TTCATGGGAA GGAAAATCTT TACGTATTGC AATGGCTGTA 
GATTCATTCA TATATTTTGC AAATATACGT CCCGATTATA TGTGGTGCTA TTTTGAAAAA 
ACTGTTGTTT TCCTAAACAA AAACAATACT AATCATATTC GTAATTCAAC GACGAATGTT 
ATCACATTCT GGAATACACT TTCTAATCAG AGTTTTCTAA AGGAAGTCGA ACCTGCATTG 
GGGATGGCTG CTTGCAGCGA GTATTGCGTT TTAGCTGTAG AATGCTTAAA TAGTAATATG 
AAGGAAAATA TTATAACTGA TGCTAAAAAT CAAAATCAGA ATGACGATAA ATGCTATCAG 
TTAATGTTGT GCAATTCTAT TGGAACTACT GTGGATTCTA AATACACTGA TATGAAACCA 
AATTTCATTG GCATTAATTC TGGACATGTA ACAATAGCCT CTTTTGATGA AGTACTCATT 
TGGCATTACA ACACTCCCAA GAGCGCAGCG AATTTGCATG GGGCGAAGGG ACGTAAAGAG 
AATCGGTTTC ATATAGATGA TACGCCAACT GGCGTCGAAA TGGCAAAAGA TTTAGTAATT 
GCTACAGGTG GAGCAAGTGG TGATAGAAAA GCCTATAAGC AACATGTTAA CATAGATCCC 
ATATGTGCAC TCGCTATGTC CGAGAAAATA TTGTTGGTGG CACGTTCCTC GGGCATTATA 
AATGAGTATA GTGTACCGAA TGTAGCACTG CGAAATCGTC ATAAGATCAA TGCAACACCT 
CATAAATTGG CTATTAATTG CAATTCTAGT CGTGCGGCCG TTATAGATGA TGTTGGTGTT 
ATGACGTTGC TAGATTTAGA GGATGATCGT CAATCACAGG TGAATTTTAA TCGAGTTGAA 
CGAAAGGATG TTTGGGCTGT TTGTTGGGCC AAGGATAATC CTTTATTACT CGCATTAATG 
GAGAAGACAC GCATGTATGT ATTTCGTGGC AATGATCCCG AAGAACCTAT TTCTTGTTCC 
GGTTATATAT GTGCTTTTGA AGATTTGGAG ATCACTAGTG TTCTGCTTGA TGACATAATT 
AGTGGCGATG AGACTCAAAA TTCTGTTAAT CATATTATAC AGTTGCGAGT TAAATCGTTG 
CGTGACACCG ATGATCTGCT GCAACATGTG GGCTTAGAAG ACGCGAAGCA ATTCATTGAA 
GATAATCCAC ACCCACGCTT ATGGCGTTTG TTGGCTGAGG CGGCATTAAA GAAACTCGAA 
TTAGGAACCG CTGAAAATGC ATTTGTTCGC TGCGCTAATT ATCAGGGGAT TCAATTAATA 
AAACGTATGC GAAATATCAC AACGCCCATG CTACAAAAAG CTGAAGTGGC CGCTTTCTAT 
GGTGAGTTCG AGGAAGCTGA AAAATTATAT ATGGATGCTG ATAGAAGAGA TTTGGCCATC 
AATCTGCGCA TTACTCTATG CGACTGGTTT CGTGCCGTTC AACTCTATCG TATGGGTTCG 
GGTGTGTCCG ACCAACAAAT GGAAATCGCT TGGCGTGAAA TTGGCAATCA TTTTGCAAGT 
TTACGTTCAT GGGAGAGCGC AAAAGAATAC TATGAAAAAT CACATCACTT AGAAGGTTTA 
ATGGATGCTC TTTACCATCT TGAGAGATTT GATGAGTTGG ATCAATGCAT TGATAAATTA 
CCTGAGAAAA GTCCATTGTT AGCAAAAATA GCCGAAATGT TGGCTTCAGT TGGAATGTGC 
ACAGAGGCAG TCAAGGCGTA CTTAAAAATG GGCGATCCTA AATCGGCGGT AAATGCTTGT 
GTTAATCTTC GACAATGGGG AGAGGCGGTA CAACTTGCTC GAAAATTCGA TATGCCACAA 
ATTGGAGAAT TGCTATGCAA GCATGCAACA CAGCTTATCA AAGAAGATCG TCTACCTGAA 
GCAATCGAAC TTCAAAAGAA AGCCGGATTC TATTTGGAAG CCGCGCGTTT GTTGGGTAAA 
CTGGCTCAAC GAGAAGCGGA GAAAGATACC AGTATGTTAC GTATTAAGAA GCTCTATATT 
CTGGCTGGAT TATTATCCGA GGAACATCTA AAATCGTTGA GTACAGCTGA ATTGAGTTAC 
AAAGTTGATA GAAATTCTAT ATTGGACACG ATGAGTCCAG AGGACGCACA TGTCGTTGAA 
CATATGTGGC ATGCGGCGGA AGCTTATCAC TTTATGATGT TGGCACAGCG TCAGTTACGT 
TTTGGTATTG CACACAGTGC GGTAGTTACT GCATTGCGCT TGTGCGATTA CGATGATATT 
TTGCCGGCAG AAGATGTATA TAATATACTC GCACTTTCCT GCTGTGCAGA TCGATCCTTT 
GGAACATGCG TCAAGGCTTT TACAAAGTTA GAAGGTTTGG AGAATATTCC GGAGTGTAGA 
GTCCATGAGT ATAAACAGCT TGCGGAAAGT ATTTTTTCCA AGTATACCAC TGAAGATACC 
AAAAATGAAA CGGTTAAATG TTACGCATGC AATTCGCCGG TGCCCGATAG TTTGCCGGTC 
TGTACAGTTT GTGGCGCTCG TTTTCCAGCA TGTGTGTCTT CAGGCAAACC TATCACTCAA 
CCCACAAGCA ATATTTGGAT TTGTGGCATC TGTCACCATT GTGCTGCTCC AGTTGAAATC 
TCACGCCATC ATACCTGTCC TCTCTGTCAT AGTTTGATAA TTTCAATGAC GCTTGAAATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196664.1
CDS	165..3767
Translation	MFVYLSKKIAIPNNVKLNCIAWNKEHGYIAVAGSEGLLKVLKLEQASSGPNKGNLAAVSNLSMNQTLDGHKEGVKVVTWNETQQKLTSSDLDGVIMVWMIYKGSWYEEMTNDRKKSTVNSMSWTSDGAKICIVYEDGAIIVGSVDGNRIWGKELKNTHLTSVQWSPDNRLILFGLANGECHLYDNQGNFAMKLNIQCVSLSPNRNFRISALCWYSGRAPSNRPVLAICFETCKLQIMRNEIDDAPVIIDTGMRNIDAEWNQDGSVLSVCGTLIDYSTSKESNQVNFYSPFGQLIRILKVPGNEISSLSWEGKSLRIAMAVDSFIYFANIRPDYMWCYFEKTVVFLNKNNTNHIRNSTTNVITFWNTLSNQSFLKEVEPALGMAACSEYCVLAVECLNSNMKENIITDAKNQNQNDDKCYQLMLCNSIGTTVDSKYTDMKPNFIGINSGHVTIASFDEVLIWHYNTPKSAANLHGAKGRKENRFHIDDTPTGVEMAKDLVIATGGASGDRKAYKQHVNIDPICALAMSEKILLVARSSGIINEYSVPNVALRNRHKINATPHKLAINCNSSRAAVIDDVGVMTLLDLEDDRQSQVNFNRVERKDVWAVCWAKDNPLLLALMEKTRMYVFRGNDPEEPISCSGYICAFEDLEITSVLLDDIISGDETQNSVNHIIQLRVKSLRDTDDLLQHVGLEDAKQFIEDNPHPRLWRLLAEAALKKLELGTAENAFVRCANYQGIQLIKRMRNITTPMLQKAEVAAFYGEFEEAEKLYMDADRRDLAINLRITLCDWFRAVQLYRMGSGVSDQQMEIAWREIGNHFASLRSWESAKEYYEKSHHLEGLMDALYHLERFDELDQCIDKLPEKSPLLAKIAEMLASVGMCTEAVKAYLKMGDPKSAVNACVNLRQWGEAVQLARKFDMPQIGELLCKHATQLIKEDRLPEAIELQKKAGFYLEAARLLGKLAQREAEKDTSMLRIKKLYILAGLLSEEHLKSLSTAELSYKVDRNSILDTMSPEDAHVVEHMWHAAEAYHFMMLAQRQLRFGIAHSAVVTALRLCDYDDILPAEDVYNILALSCCADRSFGTCVKAFTKLEGLENIPECRVHEYKQLAESIFSKYTTEDTKNETVKCYACNSPVPDSLPVCTVCGARFPACVSSGKPITQPTSNIWICGICHHCAAPVEISRHHTCPLCHSLIISMTLEI

Target ORF information:

RefSeq Version	XM_011198362.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae WD repeat-containing protein 35 (LOC105221400), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011198362.2

ORF Insert Sequence:

ATGTTTGTAT ATTTAAGTAA AAAGATTGCC ATACCAAACA ATGTAAAATT GAATTGCATA 
GCTTGGAATA AGGAGCATGG GTACATAGCT GTCGCTGGTT CTGAGGGCCT ACTTAAGGTG 
TTGAAACTAG AGCAAGCATC CAGTGGGCCA AATAAGGGGA ATCTAGCTGC CGTATCAAAC 
TTGTCCATGA ATCAAACACT GGACGGGCAT AAGGAAGGGG TTAAAGTGGT CACTTGGAAT 
GAGACGCAGC AGAAATTGAC TTCTTCGGAT CTGGATGGAG TTATTATGGT TTGGATGATC 
TATAAAGGTT CTTGGTACGA AGAGATGACT AATGATCGCA AAAAATCCAC CGTTAATAGT 
ATGAGTTGGA CATCTGATGG TGCCAAAATA TGCATAGTGT ATGAAGATGG GGCCATTATT 
GTAGGCTCAG TGGATGGAAA TCGTATATGG GGCAAGGAAC TAAAGAATAC ACATTTAACT 
AGTGTGCAAT GGAGTCCGGA TAATCGCTTG ATTTTATTTG GTTTAGCTAA TGGTGAATGT 
CATCTATATG ACAACCAAGG AAATTTTGCG ATGAAGCTAA ATATACAATG CGTCTCGCTC 
AGTCCAAATA GAAACTTTAG AATTTCGGCA CTTTGTTGGT ACAGCGGAAG AGCACCATCC 
AACAGACCAG TTCTAGCGAT TTGTTTTGAG ACGTGCAAAT TGCAAATTAT GCGTAATGAA 
ATCGATGACG CCCCAGTTAT CATCGATACT GGCATGCGAA ATATAGACGC GGAGTGGAAT 
CAAGACGGTT CTGTACTATC TGTTTGTGGA ACTCTTATAG ATTACAGTAC TTCGAAGGAA 
TCAAATCAAG TAAACTTTTA CTCGCCATTC GGACAGTTGA TACGCATTCT GAAGGTTCCA 
GGAAATGAGA TCTCTTCCCT TTCATGGGAA GGAAAATCTT TACGTATTGC AATGGCTGTA 
GATTCATTCA TATATTTTGC AAATATACGT CCCGATTATA TGTGGTGCTA TTTTGAAAAA 
ACTGTTGTTT TCCTAAACAA AAACAATACT AATCATATTC GTAATTCAAC GACGAATGTT 
ATCACATTCT GGAATACACT TTCTAATCAG AGTTTTCTAA AGGAAGTCGA ACCTGCATTG 
GGGATGGCTG CTTGCAGCGA GTATTGCGTT TTAGCTGTAG AATGCTTAAA TAGTAATATG 
AAGGAAAATA TTATAACTGA TGCTAAAAAT CAAAATCAGA ATGACGATAA ATGCTATCAG 
TTAATGTTGT GCAATTCTAT TGGAACTACT GTGGATTCTA AATACACTGA TATGAAACCA 
AATTTCATTG GCATTAATTC TGGACATGTA ACAATAGCCT CTTTTGATGA AGTACTCATT 
TGGCATTACA ACACTCCCAA GAGCGCAGCG AATTTGCATG GGGCGAAGGG ACGTAAAGAG 
AATCGGTTTC ATATAGATGA TACGCCAACT GGCGTCGAAA TGGCAAAAGA TTTAGTAATT 
GCTACAGGTG GAGCAAGTGG TGATAGAAAA GCCTATAAGC AACATGTTAA CATAGATCCC 
ATATGTGCAC TCGCTATGTC CGAGAAAATA TTGTTGGTGG CACGTTCCTC GGGCATTATA 
AATGAGTATA GTGTACCGAA TGTAGCACTG CGAAATCGTC ATAAGATCAA TGCAACACCT 
CATAAATTGG CTATTAATTG CAATTCTAGT CGTGCGGCCG TTATAGATGA TGTTGGTGTT 
ATGACGTTGC TAGATTTAGA GGATGATCGT CAATCACAGG TGAATTTTAA TCGAGTTGAA 
CGAAAGGATG TTTGGGCTGT TTGTTGGGCC AAGGATAATC CTTTATTACT CGCATTAATG 
GAGAAGACAC GCATGTATGT ATTTCGTGGC AATGATCCCG AAGAACCTAT TTCTTGTTCC 
GGTTATATAT GTGCTTTTGA AGATTTGGAG ATCACTAGTG TTCTGCTTGA TGACATAATT 
AGTGGCGATG AGACTCAAAA TTCTGTTAAT CATATTATAC AGTTGCGAGT TAAATCGTTG 
CGTGACACCG ATGATCTGCT GCAACATGTG GGCTTAGAAG ACGCGAAGCA ATTCATTGAA 
GATAATCCAC ACCCACGCTT ATGGCGTTTG TTGGCTGAGG CGGCATTAAA GAAACTCGAA 
TTAGGAACCG CTGAAAATGC ATTTGTTCGC TGCGCTAATT ATCAGGGGAT TCAATTAATA 
AAACGTATGC GAAATATCAC AACGCCCATG CTACAAAAAG CTGAAGTGGC CGCTTTCTAT 
GGTGAGTTCG AGGAAGCTGA AAAATTATAT ATGGATGCTG ATAGAAGAGA TTTGGCCATC 
AATCTGCGCA TTACTCTATG CGACTGGTTT CGTGCCGTTC AACTCTATCG TATGGGTTCG 
GGTGTGTCCG ACCAACAAAT GGAAATCGCT TGGCGTGAAA TTGGCAATCA TTTTGCAAGT 
TTACGTTCAT GGGAGAGCGC AAAAGAATAC TATGAAAAAT CACATCACTT AGAAGGTTTA 
ATGGATGCTC TTTACCATCT TGAGAGATTT GATGAGTTGG ATCAATGCAT TGATAAATTA 
CCTGAGAAAA GTCCATTGTT AGCAAAAATA GCCGAAATGT TGGCTTCAGT TGGAATGTGC 
ACAGAGGCAG TCAAGGCGTA CTTAAAAATG GGCGATCCTA AATCGGCGGT AAATGCTTGT 
GTTAATCTTC GACAATGGGG AGAGGCGGTA CAACTTGCTC GAAAATTCGA TATGCCACAA 
ATTGGAGAAT TGCTATGCAA GCATGCAACA CAGCTTATCA AAGAAGATCG TCTACCTGAA 
GCAATCGAAC TTCAAAAGAA AGCCGGATTC TATTTGGAAG CCGCGCGTTT GTTGGGTAAA 
CTGGCTCAAC GAGAAGCGGA GAAAGATACC AGTATGTTAC GTATTAAGAA GCTCTATATT 
CTGGCTGGAT TATTATCCGA GGAACATCTA AAATCGTTGA GTACAGCTGA ATTGAGTTAC 
AAAGTTGATA GAAATTCTAT ATTGGACACG ATGAGTCCAG AGGACGCACA TGTCGTTGAA 
CATATGTGGC ATGCGGCGGA AGCTTATCAC TTTATGATGT TGGCACAGCG TCAGTTACGT 
TTTGGTATTG CACACAGTGC GGTAGTTACT GCATTGCGCT TGTGCGATTA CGATGATATT 
TTGCCGGCAG AAGATGTATA TAATATACTC GCACTTTCCT GCTGTGCAGA TCGATCCTTT 
GGAACATGCG TCAAGGCTTT TACAAAGTTA GAAGGTTTGG AGAATATTCC GGAGTGTAGA 
GTCCATGAGT ATAAACAGCT TGCGGAAAGT ATTTTTTCCA AGTATACCAC TGAAGATACC 
AAAAATGAAA CGGTTAAATG TTACGCATGC AATTCGCCGG TGCCCGATAG TTTGCCGGTC 
TGTACAGTTT GTGGCGCTCG TTTTCCAGCA TGTGTGTCTT CAGGCAAACC TATCACTCAA 
CCCACAAGCA ATATTTGGAT TTGTGGCATC TGTCACCATT GTGCTGCTCC AGTTGAAATC 
TCACGCCATC ATACCTGTCC TCTCTGTCAT AGTTTGATAA TTTCAATGAC GCTTGAAATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221400 ( XM_011198362.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196664.1 Bactrocera cucurbitae WD repeat-containing protein 35 (LOC105221400), mRNA.

Price & Availability↑

CloneID	OBa193683
Clone ID Related Accession (Same CDS sequence)	XM_011198362.2 , XM_011198362.1
Accession Version	XM_011198362.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	WD repeat-containing protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTAT ATTTAAGTAA AAAGATTGCC ATACCAAACA ATGTAAAATT GAATTGCATA 
GCTTGGAATA AGGAGCATGG GTACATAGCT GTCGCTGGTT CTGAGGGCCT ACTTAAGGTG 
TTGAAACTAG AGCAAGCATC CAGTGGGCCA AATAAGGGGA ATCTAGCTGC CGTATCAAAC 
TTGTCCATGA ATCAAACACT GGACGGGCAT AAGGAAGGGG TTAAAGTGGT CACTTGGAAT 
GAGACGCAGC AGAAATTGAC TTCTTCGGAT CTGGATGGAG TTATTATGGT TTGGATGATC 
TATAAAGGTT CTTGGTACGA AGAGATGACT AATGATCGCA AAAAATCCAC CGTTAATAGT 
ATGAGTTGGA CATCTGATGG TGCCAAAATA TGCATAGTGT ATGAAGATGG GGCCATTATT 
GTAGGCTCAG TGGATGGAAA TCGTATATGG GGCAAGGAAC TAAAGAATAC ACATTTAACT 
AGTGTGCAAT GGAGTCCGGA TAATCGCTTG ATTTTATTTG GTTTAGCTAA TGGTGAATGT 
CATCTATATG ACAACCAAGG AAATTTTGCG ATGAAGCTAA ATATACAATG CGTCTCGCTC 
AGTCCAAATA GAAACTTTAG AATTTCGGCA CTTTGTTGGT ACAGCGGAAG AGCACCATCC 
AACAGACCAG TTCTAGCGAT TTGTTTTGAG ACGTGCAAAT TGCAAATTAT GCGTAATGAA 
ATCGATGACG CCCCAGTTAT CATCGATACT GGCATGCGAA ATATAGACGC GGAGTGGAAT 
CAAGACGGTT CTGTACTATC TGTTTGTGGA ACTCTTATAG ATTACAGTAC TTCGAAGGAA 
TCAAATCAAG TAAACTTTTA CTCGCCATTC GGACAGTTGA TACGCATTCT GAAGGTTCCA 
GGAAATGAGA TCTCTTCCCT TTCATGGGAA GGAAAATCTT TACGTATTGC AATGGCTGTA 
GATTCATTCA TATATTTTGC AAATATACGT CCCGATTATA TGTGGTGCTA TTTTGAAAAA 
ACTGTTGTTT TCCTAAACAA AAACAATACT AATCATATTC GTAATTCAAC GACGAATGTT 
ATCACATTCT GGAATACACT TTCTAATCAG AGTTTTCTAA AGGAAGTCGA ACCTGCATTG 
GGGATGGCTG CTTGCAGCGA GTATTGCGTT TTAGCTGTAG AATGCTTAAA TAGTAATATG 
AAGGAAAATA TTATAACTGA TGCTAAAAAT CAAAATCAGA ATGACGATAA ATGCTATCAG 
TTAATGTTGT GCAATTCTAT TGGAACTACT GTGGATTCTA AATACACTGA TATGAAACCA 
AATTTCATTG GCATTAATTC TGGACATGTA ACAATAGCCT CTTTTGATGA AGTACTCATT 
TGGCATTACA ACACTCCCAA GAGCGCAGCG AATTTGCATG GGGCGAAGGG ACGTAAAGAG 
AATCGGTTTC ATATAGATGA TACGCCAACT GGCGTCGAAA TGGCAAAAGA TTTAGTAATT 
GCTACAGGTG GAGCAAGTGG TGATAGAAAA GCCTATAAGC AACATGTTAA CATAGATCCC 
ATATGTGCAC TCGCTATGTC CGAGAAAATA TTGTTGGTGG CACGTTCCTC GGGCATTATA 
AATGAGTATA GTGTACCGAA TGTAGCACTG CGAAATCGTC ATAAGATCAA TGCAACACCT 
CATAAATTGG CTATTAATTG CAATTCTAGT CGTGCGGCCG TTATAGATGA TGTTGGTGTT 
ATGACGTTGC TAGATTTAGA GGATGATCGT CAATCACAGG TGAATTTTAA TCGAGTTGAA 
CGAAAGGATG TTTGGGCTGT TTGTTGGGCC AAGGATAATC CTTTATTACT CGCATTAATG 
GAGAAGACAC GCATGTATGT ATTTCGTGGC AATGATCCCG AAGAACCTAT TTCTTGTTCC 
GGTTATATAT GTGCTTTTGA AGATTTGGAG ATCACTAGTG TTCTGCTTGA TGACATAATT 
AGTGGCGATG AGACTCAAAA TTCTGTTAAT CATATTATAC AGTTGCGAGT TAAATCGTTG 
CGTGACACCG ATGATCTGCT GCAACATGTG GGCTTAGAAG ACGCGAAGCA ATTCATTGAA 
GATAATCCAC ACCCACGCTT ATGGCGTTTG TTGGCTGAGG CGGCATTAAA GAAACTCGAA 
TTAGGAACCG CTGAAAATGC ATTTGTTCGC TGCGCTAATT ATCAGGGGAT TCAATTAATA 
AAACGTATGC GAAATATCAC AACGCCCATG CTACAAAAAG CTGAAGTGGC CGCTTTCTAT 
GGTGAGTTCG AGGAAGCTGA AAAATTATAT ATGGATGCTG ATAGAAGAGA TTTGGCCATC 
AATCTGCGCA TTACTCTATG CGACTGGTTT CGTGCCGTTC AACTCTATCG TATGGGTTCG 
GGTGTGTCCG ACCAACAAAT GGAAATCGCT TGGCGTGAAA TTGGCAATCA TTTTGCAAGT 
TTACGTTCAT GGGAGAGCGC AAAAGAATAC TATGAAAAAT CACATCACTT AGAAGGTTTA 
ATGGATGCTC TTTACCATCT TGAGAGATTT GATGAGTTGG ATCAATGCAT TGATAAATTA 
CCTGAGAAAA GTCCATTGTT AGCAAAAATA GCCGAAATGT TGGCTTCAGT TGGAATGTGC 
ACAGAGGCAG TCAAGGCGTA CTTAAAAATG GGCGATCCTA AATCGGCGGT AAATGCTTGT 
GTTAATCTTC GACAATGGGG AGAGGCGGTA CAACTTGCTC GAAAATTCGA TATGCCACAA 
ATTGGAGAAT TGCTATGCAA GCATGCAACA CAGCTTATCA AAGAAGATCG TCTACCTGAA 
GCAATCGAAC TTCAAAAGAA AGCCGGATTC TATTTGGAAG CCGCGCGTTT GTTGGGTAAA 
CTGGCTCAAC GAGAAGCGGA GAAAGATACC AGTATGTTAC GTATTAAGAA GCTCTATATT 
CTGGCTGGAT TATTATCCGA GGAACATCTA AAATCGTTGA GTACAGCTGA ATTGAGTTAC 
AAAGTTGATA GAAATTCTAT ATTGGACACG ATGAGTCCAG AGGACGCACA TGTCGTTGAA 
CATATGTGGC ATGCGGCGGA AGCTTATCAC TTTATGATGT TGGCACAGCG TCAGTTACGT 
TTTGGTATTG CACACAGTGC GGTAGTTACT GCATTGCGCT TGTGCGATTA CGATGATATT 
TTGCCGGCAG AAGATGTATA TAATATACTC GCACTTTCCT GCTGTGCAGA TCGATCCTTT 
GGAACATGCG TCAAGGCTTT TACAAAGTTA GAAGGTTTGG AGAATATTCC GGAGTGTAGA 
GTCCATGAGT ATAAACAGCT TGCGGAAAGT ATTTTTTCCA AGTATACCAC TGAAGATACC 
AAAAATGAAA CGGTTAAATG TTACGCATGC AATTCGCCGG TGCCCGATAG TTTGCCGGTC 
TGTACAGTTT GTGGCGCTCG TTTTCCAGCA TGTGTGTCTT CAGGCAAACC TATCACTCAA 
CCCACAAGCA ATATTTGGAT TTGTGGCATC TGTCACCATT GTGCTGCTCC AGTTGAAATC 
TCACGCCATC ATACCTGTCC TCTCTGTCAT AGTTTGATAA TTTCAATGAC GCTTGAAATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196664.1
CDS	112..3714
Translation	MFVYLSKKIAIPNNVKLNCIAWNKEHGYIAVAGSEGLLKVLKLEQASSGPNKGNLAAVSNLSMNQTLDGHKEGVKVVTWNETQQKLTSSDLDGVIMVWMIYKGSWYEEMTNDRKKSTVNSMSWTSDGAKICIVYEDGAIIVGSVDGNRIWGKELKNTHLTSVQWSPDNRLILFGLANGECHLYDNQGNFAMKLNIQCVSLSPNRNFRISALCWYSGRAPSNRPVLAICFETCKLQIMRNEIDDAPVIIDTGMRNIDAEWNQDGSVLSVCGTLIDYSTSKESNQVNFYSPFGQLIRILKVPGNEISSLSWEGKSLRIAMAVDSFIYFANIRPDYMWCYFEKTVVFLNKNNTNHIRNSTTNVITFWNTLSNQSFLKEVEPALGMAACSEYCVLAVECLNSNMKENIITDAKNQNQNDDKCYQLMLCNSIGTTVDSKYTDMKPNFIGINSGHVTIASFDEVLIWHYNTPKSAANLHGAKGRKENRFHIDDTPTGVEMAKDLVIATGGASGDRKAYKQHVNIDPICALAMSEKILLVARSSGIINEYSVPNVALRNRHKINATPHKLAINCNSSRAAVIDDVGVMTLLDLEDDRQSQVNFNRVERKDVWAVCWAKDNPLLLALMEKTRMYVFRGNDPEEPISCSGYICAFEDLEITSVLLDDIISGDETQNSVNHIIQLRVKSLRDTDDLLQHVGLEDAKQFIEDNPHPRLWRLLAEAALKKLELGTAENAFVRCANYQGIQLIKRMRNITTPMLQKAEVAAFYGEFEEAEKLYMDADRRDLAINLRITLCDWFRAVQLYRMGSGVSDQQMEIAWREIGNHFASLRSWESAKEYYEKSHHLEGLMDALYHLERFDELDQCIDKLPEKSPLLAKIAEMLASVGMCTEAVKAYLKMGDPKSAVNACVNLRQWGEAVQLARKFDMPQIGELLCKHATQLIKEDRLPEAIELQKKAGFYLEAARLLGKLAQREAEKDTSMLRIKKLYILAGLLSEEHLKSLSTAELSYKVDRNSILDTMSPEDAHVVEHMWHAAEAYHFMMLAQRQLRFGIAHSAVVTALRLCDYDDILPAEDVYNILALSCCADRSFGTCVKAFTKLEGLENIPECRVHEYKQLAESIFSKYTTEDTKNETVKCYACNSPVPDSLPVCTVCGARFPACVSSGKPITQPTSNIWICGICHHCAAPVEISRHHTCPLCHSLIISMTLEI

Target ORF information:

RefSeq Version	XM_011198362.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae WD repeat-containing protein 35 (LOC105221400), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011198362.1

ORF Insert Sequence:

ATGTTTGTAT ATTTAAGTAA AAAGATTGCC ATACCAAACA ATGTAAAATT GAATTGCATA 
GCTTGGAATA AGGAGCATGG GTACATAGCT GTCGCTGGTT CTGAGGGCCT ACTTAAGGTG 
TTGAAACTAG AGCAAGCATC CAGTGGGCCA AATAAGGGGA ATCTAGCTGC CGTATCAAAC 
TTGTCCATGA ATCAAACACT GGACGGGCAT AAGGAAGGGG TTAAAGTGGT CACTTGGAAT 
GAGACGCAGC AGAAATTGAC TTCTTCGGAT CTGGATGGAG TTATTATGGT TTGGATGATC 
TATAAAGGTT CTTGGTACGA AGAGATGACT AATGATCGCA AAAAATCCAC CGTTAATAGT 
ATGAGTTGGA CATCTGATGG TGCCAAAATA TGCATAGTGT ATGAAGATGG GGCCATTATT 
GTAGGCTCAG TGGATGGAAA TCGTATATGG GGCAAGGAAC TAAAGAATAC ACATTTAACT 
AGTGTGCAAT GGAGTCCGGA TAATCGCTTG ATTTTATTTG GTTTAGCTAA TGGTGAATGT 
CATCTATATG ACAACCAAGG AAATTTTGCG ATGAAGCTAA ATATACAATG CGTCTCGCTC 
AGTCCAAATA GAAACTTTAG AATTTCGGCA CTTTGTTGGT ACAGCGGAAG AGCACCATCC 
AACAGACCAG TTCTAGCGAT TTGTTTTGAG ACGTGCAAAT TGCAAATTAT GCGTAATGAA 
ATCGATGACG CCCCAGTTAT CATCGATACT GGCATGCGAA ATATAGACGC GGAGTGGAAT 
CAAGACGGTT CTGTACTATC TGTTTGTGGA ACTCTTATAG ATTACAGTAC TTCGAAGGAA 
TCAAATCAAG TAAACTTTTA CTCGCCATTC GGACAGTTGA TACGCATTCT GAAGGTTCCA 
GGAAATGAGA TCTCTTCCCT TTCATGGGAA GGAAAATCTT TACGTATTGC AATGGCTGTA 
GATTCATTCA TATATTTTGC AAATATACGT CCCGATTATA TGTGGTGCTA TTTTGAAAAA 
ACTGTTGTTT TCCTAAACAA AAACAATACT AATCATATTC GTAATTCAAC GACGAATGTT 
ATCACATTCT GGAATACACT TTCTAATCAG AGTTTTCTAA AGGAAGTCGA ACCTGCATTG 
GGGATGGCTG CTTGCAGCGA GTATTGCGTT TTAGCTGTAG AATGCTTAAA TAGTAATATG 
AAGGAAAATA TTATAACTGA TGCTAAAAAT CAAAATCAGA ATGACGATAA ATGCTATCAG 
TTAATGTTGT GCAATTCTAT TGGAACTACT GTGGATTCTA AATACACTGA TATGAAACCA 
AATTTCATTG GCATTAATTC TGGACATGTA ACAATAGCCT CTTTTGATGA AGTACTCATT 
TGGCATTACA ACACTCCCAA GAGCGCAGCG AATTTGCATG GGGCGAAGGG ACGTAAAGAG 
AATCGGTTTC ATATAGATGA TACGCCAACT GGCGTCGAAA TGGCAAAAGA TTTAGTAATT 
GCTACAGGTG GAGCAAGTGG TGATAGAAAA GCCTATAAGC AACATGTTAA CATAGATCCC 
ATATGTGCAC TCGCTATGTC CGAGAAAATA TTGTTGGTGG CACGTTCCTC GGGCATTATA 
AATGAGTATA GTGTACCGAA TGTAGCACTG CGAAATCGTC ATAAGATCAA TGCAACACCT 
CATAAATTGG CTATTAATTG CAATTCTAGT CGTGCGGCCG TTATAGATGA TGTTGGTGTT 
ATGACGTTGC TAGATTTAGA GGATGATCGT CAATCACAGG TGAATTTTAA TCGAGTTGAA 
CGAAAGGATG TTTGGGCTGT TTGTTGGGCC AAGGATAATC CTTTATTACT CGCATTAATG 
GAGAAGACAC GCATGTATGT ATTTCGTGGC AATGATCCCG AAGAACCTAT TTCTTGTTCC 
GGTTATATAT GTGCTTTTGA AGATTTGGAG ATCACTAGTG TTCTGCTTGA TGACATAATT 
AGTGGCGATG AGACTCAAAA TTCTGTTAAT CATATTATAC AGTTGCGAGT TAAATCGTTG 
CGTGACACCG ATGATCTGCT GCAACATGTG GGCTTAGAAG ACGCGAAGCA ATTCATTGAA 
GATAATCCAC ACCCACGCTT ATGGCGTTTG TTGGCTGAGG CGGCATTAAA GAAACTCGAA 
TTAGGAACCG CTGAAAATGC ATTTGTTCGC TGCGCTAATT ATCAGGGGAT TCAATTAATA 
AAACGTATGC GAAATATCAC AACGCCCATG CTACAAAAAG CTGAAGTGGC CGCTTTCTAT 
GGTGAGTTCG AGGAAGCTGA AAAATTATAT ATGGATGCTG ATAGAAGAGA TTTGGCCATC 
AATCTGCGCA TTACTCTATG CGACTGGTTT CGTGCCGTTC AACTCTATCG TATGGGTTCG 
GGTGTGTCCG ACCAACAAAT GGAAATCGCT TGGCGTGAAA TTGGCAATCA TTTTGCAAGT 
TTACGTTCAT GGGAGAGCGC AAAAGAATAC TATGAAAAAT CACATCACTT AGAAGGTTTA 
ATGGATGCTC TTTACCATCT TGAGAGATTT GATGAGTTGG ATCAATGCAT TGATAAATTA 
CCTGAGAAAA GTCCATTGTT AGCAAAAATA GCCGAAATGT TGGCTTCAGT TGGAATGTGC 
ACAGAGGCAG TCAAGGCGTA CTTAAAAATG GGCGATCCTA AATCGGCGGT AAATGCTTGT 
GTTAATCTTC GACAATGGGG AGAGGCGGTA CAACTTGCTC GAAAATTCGA TATGCCACAA 
ATTGGAGAAT TGCTATGCAA GCATGCAACA CAGCTTATCA AAGAAGATCG TCTACCTGAA 
GCAATCGAAC TTCAAAAGAA AGCCGGATTC TATTTGGAAG CCGCGCGTTT GTTGGGTAAA 
CTGGCTCAAC GAGAAGCGGA GAAAGATACC AGTATGTTAC GTATTAAGAA GCTCTATATT 
CTGGCTGGAT TATTATCCGA GGAACATCTA AAATCGTTGA GTACAGCTGA ATTGAGTTAC 
AAAGTTGATA GAAATTCTAT ATTGGACACG ATGAGTCCAG AGGACGCACA TGTCGTTGAA 
CATATGTGGC ATGCGGCGGA AGCTTATCAC TTTATGATGT TGGCACAGCG TCAGTTACGT 
TTTGGTATTG CACACAGTGC GGTAGTTACT GCATTGCGCT TGTGCGATTA CGATGATATT 
TTGCCGGCAG AAGATGTATA TAATATACTC GCACTTTCCT GCTGTGCAGA TCGATCCTTT 
GGAACATGCG TCAAGGCTTT TACAAAGTTA GAAGGTTTGG AGAATATTCC GGAGTGTAGA 
GTCCATGAGT ATAAACAGCT TGCGGAAAGT ATTTTTTCCA AGTATACCAC TGAAGATACC 
AAAAATGAAA CGGTTAAATG TTACGCATGC AATTCGCCGG TGCCCGATAG TTTGCCGGTC 
TGTACAGTTT GTGGCGCTCG TTTTCCAGCA TGTGTGTCTT CAGGCAAACC TATCACTCAA 
CCCACAAGCA ATATTTGGAT TTGTGGCATC TGTCACCATT GTGCTGCTCC AGTTGAAATC 
TCACGCCATC ATACCTGTCC TCTCTGTCAT AGTTTGATAA TTTCAATGAC GCTTGAAATT 
TAA