LOC105213286 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105213286
Entrez Gene ID	105213286
Full Name	zinc finger protein 800
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011185997.1	XP_011184299.1	uncharacterized protein LOC105213286
XM_011185997.2	XP_011184299.1	uncharacterized protein LOC105213286

Pathways from BioSystems
Gene Ontology

The following LOC105213286 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105213286 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa199764	XM_011185997.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105213286 (LOC105213286), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa199764	XM_011185997.1	Bactrocera cucurbitae uncharacterized LOC105213286 (LOC105213286), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105213286 ( XM_011185997.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011184299.1 Bactrocera cucurbitae uncharacterized LOC105213286 (LOC105213286), mRNA.

Price & Availability↑

CloneID	OBa199764
Clone ID Related Accession (Same CDS sequence)	XM_011185997.1 , XM_011185997.2
Accession Version	XM_011185997.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105213286
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863715.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGC GCACGTCCGA CTACTATAGC TCCGATACGG AACAAATGGT GGCCTCTGAG 
GATATGGGGG CTGCTAAAAC GCTTTCAATT GACGAATATC AAGATATATA TCTGATGCGT 
CCGCAAACGA AGTTGATCTT TGATAGTGGT TTTAAGGAAG CAAAACACAT CTATGATACT 
GGTGATGAAG AGGTGCAACA ATTATTGTTG AAGGAATGTA GACTTATATA TGAGTGTAAA 
TTGTGTCGCA ATATGTTCCG TAGTCTGACG CAGTTCATCG GTCATAAAAG AGAATTCTGT 
CGCAGCGCAG TACCCAGCGA CGGTGACATA TCTGAGGGCG ATAAATACAA TGGATTCTCC 
AGCAATGTAA TGGTTACCAA AGAAATTTGT AATAATAAAA ATAATAGATG TGAATCTGTT 
TTGCAAACTA CTGAAGAGGC TGTGCAACAG ACCAGAACTC CTTGTGAAAG TAATGCACTT 
ATGGATGAAA AAATATCGGG AAATTGCACG AATCAGCCAG TTGAAAATGA GAAAATAAAA 
ACTATATTAC ACTGTCCTCA AGAGCATTGC ATGCTGAATT TTTCAAACAC CAAAGAACTT 
GAATTGCATT TCTCACAAGA TCACACTAAA TCTATGGTGT TCACATCATT TGCTGAACAC 
AAAATGGACA ATCAAAAGCA AGCACTTTCT GATACCGTTG TTCTTGTCAG ATCGCAGCGT 
ATGTCAACAA ATAATTGCCA CGCATGTGAA CATTGTGAAA AAACTTTTGA ACGCGCTGAA 
GCTCTATCCA CACATCTACT ACATTGTCCG GTAAAGCATA ATATATTGAG TAAAACTCTT 
GCCACGGGAA CAGGAGACAA TATTGCAACA AAACGCTTGA AATTGCAAGA GTCGGCTCAG 
GGTCTGATTT GTAATGGCAA AGAAGCGCCG GACTTGATTC CAGAAATAGT GCATATAACC 
GATAGTGTAG CAACGGATGC GCAAAATGTT GTAACTGTGA AGCAGGAGTA TGTTGTCTCA 
GAGGGCAGTA CAACAGGCAA CACAAGCGTT ACAGCAAAGA AAAGGAAATT GAAGAATCGT 
AGAGTTGTCA CAAATAAAAT TAGCGCGGAT ATCGTTGGAC AAACGGGACT TGACCACATT 
TCGCCTATAA AACAGGAACC TTGCATTGAA GTAACTCGCA TGCCATATGC CAAAATTAAA 
TCGGAATTAA ATCTGGCGGA TGGCGGTAGT GATACAGAGC CAAAGAAATT GCTTTCCACT 
TGCACCTATT GCGGCAAGTC GTTTGAACGG CGCGCTGCTC TATCGACACA TCTGCAACAT 
TGTCCGGTGA AGTTTAGCGT CATTGAAAAG CACACAATTT ATGGCATGAG TACATGTTCG 
AAACGTACAA AATTGCATTC AACCTCCGAG AAGACAGCAA AAGACATGCG CCAACAAAAT 
GTTGTCGAAG ATCCTGATAC TGATCTCTTA AATGAAATGT TTGCAAATTT GGGTAGACAG 
ACATTCGGCG TGGGCCTGCA AACGGTTACA ATTGATCACG ATGATACGAC AGCTTCCGAC 
GGTACTGATG ATGCCAAGGA GGGGGAGGAA GAGAATGGAG AAATGCTTAC ACCGACTAAG 
CTGGAAGAAA CACTGCCCGC AGATCAAAGT GTTGAACTTT CCGCTAGCAT GACAACAACA 
TTTGAGAGTT CACCTGATGC ACACGTTGAG GAGGACGCGG AGAAAAAGTC GAGTCGTCGT 
GTGCGTTCAG CGCCAATGGA GAAACGTTTA CTCTGCCGTT GTAAGATCTG CTACAAGCAA 
TTCAACGCAC TCAGCAACTT GCGTCGTCAC ATTTCGATGT TCCACTATCG GGCGCAGCGT 
TTCGGTTGCA CATTGTGCGA TTATCGCGCT TTCCGTCGCT ACGACATTGT CAACCATTTG 
GGCAATACGC ACGCCATGAA GGGCGATCCC GAGGCGATGT CAATTGAATT TGTTTCGGAG 
CACGAAGTAA ACTATAGTCG CGAAGATGTT GAGGGCGACA TTGTTGTGCT TGATGAAGGC 
AGTGAGAACT TCAGTGCGTT AAATGAGTCA ACGGAAGATG TTGAAAATAG CATTGAGCCA 
AGACAAAATC TGAAACGCTT AAAGCGTAAC AAAACTATAT CGATCTCAAG TGGGCTTAGC 
GTTGCGCTAA ATAAGCTTAC GCGTCAAAAA GTCAAACGGT CACAATCGCA ACATGAAACA 
TGTAGTGGTG CCAAAACGCC GGCAAGGCGT CCCATACGGA ATCGCATTAA GCCGGAAAAT 
AAAGACTTCG TATACGATCT CTCCAGTTTG GTGAAGAAGG AAAACACTGA TGTACCAAAC 
GACATTACGC GCAGTCTGCG TCGCCGTAAT ACTATGCATG TCGGTCCAAT GGAACCAGCT 
GCAAATTGTT TAGAATCACT TTTGGGTCCC GCAACCGGTT GGGAACATAT ACGTGGCGCC 
GCGCAGCGCC TCGCTGCGTC CGCCATAAGT AGCGGTGCGG CAGCATCTGA TACACAACCC 
GAATTGCCCA CAGAGAGGCC ACAAATGCGT CAGCGTTTGA TTTCGACACA TCGCGCGGAT 
AATCATGCAG TGCCGGTGAT CGAGGCCAGT GAATTGGAAG CTGCCCGTCT ACATTCTACC 
TTACTCGAGG ACACATTCCT TGATAAAGTG GCCAAATCAT CGCCAACATC CTTTAAAATC 
AAACCACTGT TATCACTACA TACCAGCACA TTGAATCGAT TGTTGGAAAA AGTCGATTCA 
TCGCTGCATC AAAGCGCCGC CAATGGTAAA TCCTCGCCCA GCAGCGAGAC TACGTGTAGT 
GATGAGAATG TCATCAGTAG CATGCATATC TCCCCGCCTC CGTCAATTCC TCGCGCTAAC 
GCTCCGCCAC CCACGCCGCG CAAACGCATC TCAATGTTGG ATCGTCTGCG GGACGATACC 
TTCAGATACC GCGACAGTCT ACTACGCTCA GCATTGGAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011184299.1
CDS	483..3467
Translation	MRKRTSDYYSSDTEQMVASEDMGAAKTLSIDEYQDIYLMRPQTKLIFDSGFKEAKHIYDTGDEEVQQLLLKECRLIYECKLCRNMFRSLTQFIGHKREFCRSAVPSDGDISEGDKYNGFSSNVMVTKEICNNKNNRCESVLQTTEEAVQQTRTPCESNALMDEKISGNCTNQPVENEKIKTILHCPQEHCMLNFSNTKELELHFSQDHTKSMVFTSFAEHKMDNQKQALSDTVVLVRSQRMSTNNCHACEHCEKTFERAEALSTHLLHCPVKHNILSKTLATGTGDNIATKRLKLQESAQGLICNGKEAPDLIPEIVHITDSVATDAQNVVTVKQEYVVSEGSTTGNTSVTAKKRKLKNRRVVTNKISADIVGQTGLDHISPIKQEPCIEVTRMPYAKIKSELNLADGGSDTEPKKLLSTCTYCGKSFERRAALSTHLQHCPVKFSVIEKHTIYGMSTCSKRTKLHSTSEKTAKDMRQQNVVEDPDTDLLNEMFANLGRQTFGVGLQTVTIDHDDTTASDGTDDAKEGEEENGEMLTPTKLEETLPADQSVELSASMTTTFESSPDAHVEEDAEKKSSRRVRSAPMEKRLLCRCKICYKQFNALSNLRRHISMFHYRAQRFGCTLCDYRAFRRYDIVNHLGNTHAMKGDPEAMSIEFVSEHEVNYSREDVEGDIVVLDEGSENFSALNESTEDVENSIEPRQNLKRLKRNKTISISSGLSVALNKLTRQKVKRSQSQHETCSGAKTPARRPIRNRIKPENKDFVYDLSSLVKKENTDVPNDITRSLRRRNTMHVGPMEPAANCLESLLGPATGWEHIRGAAQRLAASAISSGAAASDTQPELPTERPQMRQRLISTHRADNHAVPVIEASELEAARLHSTLLEDTFLDKVAKSSPTSFKIKPLLSLHTSTLNRLLEKVDSSLHQSAANGKSSPSSETTCSDENVISSMHISPPPSIPRANAPPPTPRKRISMLDRLRDDTFRYRDSLLRSALEN

Target ORF information:

RefSeq Version	XM_011185997.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105213286 (LOC105213286), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185997.1

ORF Insert Sequence:

ATGAGAAAGC GCACGTCCGA CTACTATAGC TCCGATACGG AACAAATGGT GGCCTCTGAG 
GATATGGGGG CTGCTAAAAC GCTTTCAATT GACGAATATC AAGATATATA TCTGATGCGT 
CCGCAAACGA AGTTGATCTT TGATAGTGGT TTTAAGGAAG CAAAACACAT CTATGATACT 
GGTGATGAAG AGGTGCAACA ATTATTGTTG AAGGAATGTA GACTTATATA TGAGTGTAAA 
TTGTGTCGCA ATATGTTCCG TAGTCTGACG CAGTTCATCG GTCATAAAAG AGAATTCTGT 
CGCAGCGCAG TACCCAGCGA CGGTGACATA TCTGAGGGCG ATAAATACAA TGGATTCTCC 
AGCAATGTAA TGGTTACCAA AGAAATTTGT AATAATAAAA ATAATAGATG TGAATCTGTT 
TTGCAAACTA CTGAAGAGGC TGTGCAACAG ACCAGAACTC CTTGTGAAAG TAATGCACTT 
ATGGATGAAA AAATATCGGG AAATTGCACG AATCAGCCAG TTGAAAATGA GAAAATAAAA 
ACTATATTAC ACTGTCCTCA AGAGCATTGC ATGCTGAATT TTTCAAACAC CAAAGAACTT 
GAATTGCATT TCTCACAAGA TCACACTAAA TCTATGGTGT TCACATCATT TGCTGAACAC 
AAAATGGACA ATCAAAAGCA AGCACTTTCT GATACCGTTG TTCTTGTCAG ATCGCAGCGT 
ATGTCAACAA ATAATTGCCA CGCATGTGAA CATTGTGAAA AAACTTTTGA ACGCGCTGAA 
GCTCTATCCA CACATCTACT ACATTGTCCG GTAAAGCATA ATATATTGAG TAAAACTCTT 
GCCACGGGAA CAGGAGACAA TATTGCAACA AAACGCTTGA AATTGCAAGA GTCGGCTCAG 
GGTCTGATTT GTAATGGCAA AGAAGCGCCG GACTTGATTC CAGAAATAGT GCATATAACC 
GATAGTGTAG CAACGGATGC GCAAAATGTT GTAACTGTGA AGCAGGAGTA TGTTGTCTCA 
GAGGGCAGTA CAACAGGCAA CACAAGCGTT ACAGCAAAGA AAAGGAAATT GAAGAATCGT 
AGAGTTGTCA CAAATAAAAT TAGCGCGGAT ATCGTTGGAC AAACGGGACT TGACCACATT 
TCGCCTATAA AACAGGAACC TTGCATTGAA GTAACTCGCA TGCCATATGC CAAAATTAAA 
TCGGAATTAA ATCTGGCGGA TGGCGGTAGT GATACAGAGC CAAAGAAATT GCTTTCCACT 
TGCACCTATT GCGGCAAGTC GTTTGAACGG CGCGCTGCTC TATCGACACA TCTGCAACAT 
TGTCCGGTGA AGTTTAGCGT CATTGAAAAG CACACAATTT ATGGCATGAG TACATGTTCG 
AAACGTACAA AATTGCATTC AACCTCCGAG AAGACAGCAA AAGACATGCG CCAACAAAAT 
GTTGTCGAAG ATCCTGATAC TGATCTCTTA AATGAAATGT TTGCAAATTT GGGTAGACAG 
ACATTCGGCG TGGGCCTGCA AACGGTTACA ATTGATCACG ATGATACGAC AGCTTCCGAC 
GGTACTGATG ATGCCAAGGA GGGGGAGGAA GAGAATGGAG AAATGCTTAC ACCGACTAAG 
CTGGAAGAAA CACTGCCCGC AGATCAAAGT GTTGAACTTT CCGCTAGCAT GACAACAACA 
TTTGAGAGTT CACCTGATGC ACACGTTGAG GAGGACGCGG AGAAAAAGTC GAGTCGTCGT 
GTGCGTTCAG CGCCAATGGA GAAACGTTTA CTCTGCCGTT GTAAGATCTG CTACAAGCAA 
TTCAACGCAC TCAGCAACTT GCGTCGTCAC ATTTCGATGT TCCACTATCG GGCGCAGCGT 
TTCGGTTGCA CATTGTGCGA TTATCGCGCT TTCCGTCGCT ACGACATTGT CAACCATTTG 
GGCAATACGC ACGCCATGAA GGGCGATCCC GAGGCGATGT CAATTGAATT TGTTTCGGAG 
CACGAAGTAA ACTATAGTCG CGAAGATGTT GAGGGCGACA TTGTTGTGCT TGATGAAGGC 
AGTGAGAACT TCAGTGCGTT AAATGAGTCA ACGGAAGATG TTGAAAATAG CATTGAGCCA 
AGACAAAATC TGAAACGCTT AAAGCGTAAC AAAACTATAT CGATCTCAAG TGGGCTTAGC 
GTTGCGCTAA ATAAGCTTAC GCGTCAAAAA GTCAAACGGT CACAATCGCA ACATGAAACA 
TGTAGTGGTG CCAAAACGCC GGCAAGGCGT CCCATACGGA ATCGCATTAA GCCGGAAAAT 
AAAGACTTCG TATACGATCT CTCCAGTTTG GTGAAGAAGG AAAACACTGA TGTACCAAAC 
GACATTACGC GCAGTCTGCG TCGCCGTAAT ACTATGCATG TCGGTCCAAT GGAACCAGCT 
GCAAATTGTT TAGAATCACT TTTGGGTCCC GCAACCGGTT GGGAACATAT ACGTGGCGCC 
GCGCAGCGCC TCGCTGCGTC CGCCATAAGT AGCGGTGCGG CAGCATCTGA TACACAACCC 
GAATTGCCCA CAGAGAGGCC ACAAATGCGT CAGCGTTTGA TTTCGACACA TCGCGCGGAT 
AATCATGCAG TGCCGGTGAT CGAGGCCAGT GAATTGGAAG CTGCCCGTCT ACATTCTACC 
TTACTCGAGG ACACATTCCT TGATAAAGTG GCCAAATCAT CGCCAACATC CTTTAAAATC 
AAACCACTGT TATCACTACA TACCAGCACA TTGAATCGAT TGTTGGAAAA AGTCGATTCA 
TCGCTGCATC AAAGCGCCGC CAATGGTAAA TCCTCGCCCA GCAGCGAGAC TACGTGTAGT 
GATGAGAATG TCATCAGTAG CATGCATATC TCCCCGCCTC CGTCAATTCC TCGCGCTAAC 
GCTCCGCCAC CCACGCCGCG CAAACGCATC TCAATGTTGG ATCGTCTGCG GGACGATACC 
TTCAGATACC GCGACAGTCT ACTACGCTCA GCATTGGAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105213286 ( XM_011185997.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011184299.1 Zeugodacus cucurbitae uncharacterized LOC105213286 (LOC105213286), mRNA.

Price & Availability↑

CloneID	OBa199764
Clone ID Related Accession (Same CDS sequence)	XM_011185997.1 , XM_011185997.2
Accession Version	XM_011185997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105213286
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863715.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGC GCACGTCCGA CTACTATAGC TCCGATACGG AACAAATGGT GGCCTCTGAG 
GATATGGGGG CTGCTAAAAC GCTTTCAATT GACGAATATC AAGATATATA TCTGATGCGT 
CCGCAAACGA AGTTGATCTT TGATAGTGGT TTTAAGGAAG CAAAACACAT CTATGATACT 
GGTGATGAAG AGGTGCAACA ATTATTGTTG AAGGAATGTA GACTTATATA TGAGTGTAAA 
TTGTGTCGCA ATATGTTCCG TAGTCTGACG CAGTTCATCG GTCATAAAAG AGAATTCTGT 
CGCAGCGCAG TACCCAGCGA CGGTGACATA TCTGAGGGCG ATAAATACAA TGGATTCTCC 
AGCAATGTAA TGGTTACCAA AGAAATTTGT AATAATAAAA ATAATAGATG TGAATCTGTT 
TTGCAAACTA CTGAAGAGGC TGTGCAACAG ACCAGAACTC CTTGTGAAAG TAATGCACTT 
ATGGATGAAA AAATATCGGG AAATTGCACG AATCAGCCAG TTGAAAATGA GAAAATAAAA 
ACTATATTAC ACTGTCCTCA AGAGCATTGC ATGCTGAATT TTTCAAACAC CAAAGAACTT 
GAATTGCATT TCTCACAAGA TCACACTAAA TCTATGGTGT TCACATCATT TGCTGAACAC 
AAAATGGACA ATCAAAAGCA AGCACTTTCT GATACCGTTG TTCTTGTCAG ATCGCAGCGT 
ATGTCAACAA ATAATTGCCA CGCATGTGAA CATTGTGAAA AAACTTTTGA ACGCGCTGAA 
GCTCTATCCA CACATCTACT ACATTGTCCG GTAAAGCATA ATATATTGAG TAAAACTCTT 
GCCACGGGAA CAGGAGACAA TATTGCAACA AAACGCTTGA AATTGCAAGA GTCGGCTCAG 
GGTCTGATTT GTAATGGCAA AGAAGCGCCG GACTTGATTC CAGAAATAGT GCATATAACC 
GATAGTGTAG CAACGGATGC GCAAAATGTT GTAACTGTGA AGCAGGAGTA TGTTGTCTCA 
GAGGGCAGTA CAACAGGCAA CACAAGCGTT ACAGCAAAGA AAAGGAAATT GAAGAATCGT 
AGAGTTGTCA CAAATAAAAT TAGCGCGGAT ATCGTTGGAC AAACGGGACT TGACCACATT 
TCGCCTATAA AACAGGAACC TTGCATTGAA GTAACTCGCA TGCCATATGC CAAAATTAAA 
TCGGAATTAA ATCTGGCGGA TGGCGGTAGT GATACAGAGC CAAAGAAATT GCTTTCCACT 
TGCACCTATT GCGGCAAGTC GTTTGAACGG CGCGCTGCTC TATCGACACA TCTGCAACAT 
TGTCCGGTGA AGTTTAGCGT CATTGAAAAG CACACAATTT ATGGCATGAG TACATGTTCG 
AAACGTACAA AATTGCATTC AACCTCCGAG AAGACAGCAA AAGACATGCG CCAACAAAAT 
GTTGTCGAAG ATCCTGATAC TGATCTCTTA AATGAAATGT TTGCAAATTT GGGTAGACAG 
ACATTCGGCG TGGGCCTGCA AACGGTTACA ATTGATCACG ATGATACGAC AGCTTCCGAC 
GGTACTGATG ATGCCAAGGA GGGGGAGGAA GAGAATGGAG AAATGCTTAC ACCGACTAAG 
CTGGAAGAAA CACTGCCCGC AGATCAAAGT GTTGAACTTT CCGCTAGCAT GACAACAACA 
TTTGAGAGTT CACCTGATGC ACACGTTGAG GAGGACGCGG AGAAAAAGTC GAGTCGTCGT 
GTGCGTTCAG CGCCAATGGA GAAACGTTTA CTCTGCCGTT GTAAGATCTG CTACAAGCAA 
TTCAACGCAC TCAGCAACTT GCGTCGTCAC ATTTCGATGT TCCACTATCG GGCGCAGCGT 
TTCGGTTGCA CATTGTGCGA TTATCGCGCT TTCCGTCGCT ACGACATTGT CAACCATTTG 
GGCAATACGC ACGCCATGAA GGGCGATCCC GAGGCGATGT CAATTGAATT TGTTTCGGAG 
CACGAAGTAA ACTATAGTCG CGAAGATGTT GAGGGCGACA TTGTTGTGCT TGATGAAGGC 
AGTGAGAACT TCAGTGCGTT AAATGAGTCA ACGGAAGATG TTGAAAATAG CATTGAGCCA 
AGACAAAATC TGAAACGCTT AAAGCGTAAC AAAACTATAT CGATCTCAAG TGGGCTTAGC 
GTTGCGCTAA ATAAGCTTAC GCGTCAAAAA GTCAAACGGT CACAATCGCA ACATGAAACA 
TGTAGTGGTG CCAAAACGCC GGCAAGGCGT CCCATACGGA ATCGCATTAA GCCGGAAAAT 
AAAGACTTCG TATACGATCT CTCCAGTTTG GTGAAGAAGG AAAACACTGA TGTACCAAAC 
GACATTACGC GCAGTCTGCG TCGCCGTAAT ACTATGCATG TCGGTCCAAT GGAACCAGCT 
GCAAATTGTT TAGAATCACT TTTGGGTCCC GCAACCGGTT GGGAACATAT ACGTGGCGCC 
GCGCAGCGCC TCGCTGCGTC CGCCATAAGT AGCGGTGCGG CAGCATCTGA TACACAACCC 
GAATTGCCCA CAGAGAGGCC ACAAATGCGT CAGCGTTTGA TTTCGACACA TCGCGCGGAT 
AATCATGCAG TGCCGGTGAT CGAGGCCAGT GAATTGGAAG CTGCCCGTCT ACATTCTACC 
TTACTCGAGG ACACATTCCT TGATAAAGTG GCCAAATCAT CGCCAACATC CTTTAAAATC 
AAACCACTGT TATCACTACA TACCAGCACA TTGAATCGAT TGTTGGAAAA AGTCGATTCA 
TCGCTGCATC AAAGCGCCGC CAATGGTAAA TCCTCGCCCA GCAGCGAGAC TACGTGTAGT 
GATGAGAATG TCATCAGTAG CATGCATATC TCCCCGCCTC CGTCAATTCC TCGCGCTAAC 
GCTCCGCCAC CCACGCCGCG CAAACGCATC TCAATGTTGG ATCGTCTGCG GGACGATACC 
TTCAGATACC GCGACAGTCT ACTACGCTCA GCATTGGAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011184299.1
CDS	460..3444
Translation	MRKRTSDYYSSDTEQMVASEDMGAAKTLSIDEYQDIYLMRPQTKLIFDSGFKEAKHIYDTGDEEVQQLLLKECRLIYECKLCRNMFRSLTQFIGHKREFCRSAVPSDGDISEGDKYNGFSSNVMVTKEICNNKNNRCESVLQTTEEAVQQTRTPCESNALMDEKISGNCTNQPVENEKIKTILHCPQEHCMLNFSNTKELELHFSQDHTKSMVFTSFAEHKMDNQKQALSDTVVLVRSQRMSTNNCHACEHCEKTFERAEALSTHLLHCPVKHNILSKTLATGTGDNIATKRLKLQESAQGLICNGKEAPDLIPEIVHITDSVATDAQNVVTVKQEYVVSEGSTTGNTSVTAKKRKLKNRRVVTNKISADIVGQTGLDHISPIKQEPCIEVTRMPYAKIKSELNLADGGSDTEPKKLLSTCTYCGKSFERRAALSTHLQHCPVKFSVIEKHTIYGMSTCSKRTKLHSTSEKTAKDMRQQNVVEDPDTDLLNEMFANLGRQTFGVGLQTVTIDHDDTTASDGTDDAKEGEEENGEMLTPTKLEETLPADQSVELSASMTTTFESSPDAHVEEDAEKKSSRRVRSAPMEKRLLCRCKICYKQFNALSNLRRHISMFHYRAQRFGCTLCDYRAFRRYDIVNHLGNTHAMKGDPEAMSIEFVSEHEVNYSREDVEGDIVVLDEGSENFSALNESTEDVENSIEPRQNLKRLKRNKTISISSGLSVALNKLTRQKVKRSQSQHETCSGAKTPARRPIRNRIKPENKDFVYDLSSLVKKENTDVPNDITRSLRRRNTMHVGPMEPAANCLESLLGPATGWEHIRGAAQRLAASAISSGAAASDTQPELPTERPQMRQRLISTHRADNHAVPVIEASELEAARLHSTLLEDTFLDKVAKSSPTSFKIKPLLSLHTSTLNRLLEKVDSSLHQSAANGKSSPSSETTCSDENVISSMHISPPPSIPRANAPPPTPRKRISMLDRLRDDTFRYRDSLLRSALEN

Target ORF information:

RefSeq Version	XM_011185997.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105213286 (LOC105213286), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185997.2

ORF Insert Sequence:

ATGAGAAAGC GCACGTCCGA CTACTATAGC TCCGATACGG AACAAATGGT GGCCTCTGAG 
GATATGGGGG CTGCTAAAAC GCTTTCAATT GACGAATATC AAGATATATA TCTGATGCGT 
CCGCAAACGA AGTTGATCTT TGATAGTGGT TTTAAGGAAG CAAAACACAT CTATGATACT 
GGTGATGAAG AGGTGCAACA ATTATTGTTG AAGGAATGTA GACTTATATA TGAGTGTAAA 
TTGTGTCGCA ATATGTTCCG TAGTCTGACG CAGTTCATCG GTCATAAAAG AGAATTCTGT 
CGCAGCGCAG TACCCAGCGA CGGTGACATA TCTGAGGGCG ATAAATACAA TGGATTCTCC 
AGCAATGTAA TGGTTACCAA AGAAATTTGT AATAATAAAA ATAATAGATG TGAATCTGTT 
TTGCAAACTA CTGAAGAGGC TGTGCAACAG ACCAGAACTC CTTGTGAAAG TAATGCACTT 
ATGGATGAAA AAATATCGGG AAATTGCACG AATCAGCCAG TTGAAAATGA GAAAATAAAA 
ACTATATTAC ACTGTCCTCA AGAGCATTGC ATGCTGAATT TTTCAAACAC CAAAGAACTT 
GAATTGCATT TCTCACAAGA TCACACTAAA TCTATGGTGT TCACATCATT TGCTGAACAC 
AAAATGGACA ATCAAAAGCA AGCACTTTCT GATACCGTTG TTCTTGTCAG ATCGCAGCGT 
ATGTCAACAA ATAATTGCCA CGCATGTGAA CATTGTGAAA AAACTTTTGA ACGCGCTGAA 
GCTCTATCCA CACATCTACT ACATTGTCCG GTAAAGCATA ATATATTGAG TAAAACTCTT 
GCCACGGGAA CAGGAGACAA TATTGCAACA AAACGCTTGA AATTGCAAGA GTCGGCTCAG 
GGTCTGATTT GTAATGGCAA AGAAGCGCCG GACTTGATTC CAGAAATAGT GCATATAACC 
GATAGTGTAG CAACGGATGC GCAAAATGTT GTAACTGTGA AGCAGGAGTA TGTTGTCTCA 
GAGGGCAGTA CAACAGGCAA CACAAGCGTT ACAGCAAAGA AAAGGAAATT GAAGAATCGT 
AGAGTTGTCA CAAATAAAAT TAGCGCGGAT ATCGTTGGAC AAACGGGACT TGACCACATT 
TCGCCTATAA AACAGGAACC TTGCATTGAA GTAACTCGCA TGCCATATGC CAAAATTAAA 
TCGGAATTAA ATCTGGCGGA TGGCGGTAGT GATACAGAGC CAAAGAAATT GCTTTCCACT 
TGCACCTATT GCGGCAAGTC GTTTGAACGG CGCGCTGCTC TATCGACACA TCTGCAACAT 
TGTCCGGTGA AGTTTAGCGT CATTGAAAAG CACACAATTT ATGGCATGAG TACATGTTCG 
AAACGTACAA AATTGCATTC AACCTCCGAG AAGACAGCAA AAGACATGCG CCAACAAAAT 
GTTGTCGAAG ATCCTGATAC TGATCTCTTA AATGAAATGT TTGCAAATTT GGGTAGACAG 
ACATTCGGCG TGGGCCTGCA AACGGTTACA ATTGATCACG ATGATACGAC AGCTTCCGAC 
GGTACTGATG ATGCCAAGGA GGGGGAGGAA GAGAATGGAG AAATGCTTAC ACCGACTAAG 
CTGGAAGAAA CACTGCCCGC AGATCAAAGT GTTGAACTTT CCGCTAGCAT GACAACAACA 
TTTGAGAGTT CACCTGATGC ACACGTTGAG GAGGACGCGG AGAAAAAGTC GAGTCGTCGT 
GTGCGTTCAG CGCCAATGGA GAAACGTTTA CTCTGCCGTT GTAAGATCTG CTACAAGCAA 
TTCAACGCAC TCAGCAACTT GCGTCGTCAC ATTTCGATGT TCCACTATCG GGCGCAGCGT 
TTCGGTTGCA CATTGTGCGA TTATCGCGCT TTCCGTCGCT ACGACATTGT CAACCATTTG 
GGCAATACGC ACGCCATGAA GGGCGATCCC GAGGCGATGT CAATTGAATT TGTTTCGGAG 
CACGAAGTAA ACTATAGTCG CGAAGATGTT GAGGGCGACA TTGTTGTGCT TGATGAAGGC 
AGTGAGAACT TCAGTGCGTT AAATGAGTCA ACGGAAGATG TTGAAAATAG CATTGAGCCA 
AGACAAAATC TGAAACGCTT AAAGCGTAAC AAAACTATAT CGATCTCAAG TGGGCTTAGC 
GTTGCGCTAA ATAAGCTTAC GCGTCAAAAA GTCAAACGGT CACAATCGCA ACATGAAACA 
TGTAGTGGTG CCAAAACGCC GGCAAGGCGT CCCATACGGA ATCGCATTAA GCCGGAAAAT 
AAAGACTTCG TATACGATCT CTCCAGTTTG GTGAAGAAGG AAAACACTGA TGTACCAAAC 
GACATTACGC GCAGTCTGCG TCGCCGTAAT ACTATGCATG TCGGTCCAAT GGAACCAGCT 
GCAAATTGTT TAGAATCACT TTTGGGTCCC GCAACCGGTT GGGAACATAT ACGTGGCGCC 
GCGCAGCGCC TCGCTGCGTC CGCCATAAGT AGCGGTGCGG CAGCATCTGA TACACAACCC 
GAATTGCCCA CAGAGAGGCC ACAAATGCGT CAGCGTTTGA TTTCGACACA TCGCGCGGAT 
AATCATGCAG TGCCGGTGAT CGAGGCCAGT GAATTGGAAG CTGCCCGTCT ACATTCTACC 
TTACTCGAGG ACACATTCCT TGATAAAGTG GCCAAATCAT CGCCAACATC CTTTAAAATC 
AAACCACTGT TATCACTACA TACCAGCACA TTGAATCGAT TGTTGGAAAA AGTCGATTCA 
TCGCTGCATC AAAGCGCCGC CAATGGTAAA TCCTCGCCCA GCAGCGAGAC TACGTGTAGT 
GATGAGAATG TCATCAGTAG CATGCATATC TCCCCGCCTC CGTCAATTCC TCGCGCTAAC 
GCTCCGCCAC CCACGCCGCG CAAACGCATC TCAATGTTGG ATCGTCTGCG GGACGATACC 
TTCAGATACC GCGACAGTCT ACTACGCTCA GCATTGGAGA ACTAA