Espn gene cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

mRNA	Protein	Name
XM_011185142.1	XP_011183444.1	uncharacterized protein LOC105212834 isoform X3
XM_011185145.1	XP_011183447.1	uncharacterized protein LOC105212834 isoform X5
XM_011185141.2	XP_011183443.1	uncharacterized protein Espn isoform X2
XM_011185143.2	XP_011183445.1	uncharacterized protein Espn isoform X3
XM_011185139.1	XP_011183441.1	uncharacterized protein LOC105212834 isoform X1
XM_011185140.1	XP_011183442.1	uncharacterized protein LOC105212834 isoform X1
XM_011185144.1	XP_011183446.1	uncharacterized protein LOC105212834 isoform X4
XM_011185139.2	XP_011183441.1	uncharacterized protein Espn isoform X1
XM_029040633.1	XP_028896466.1	uncharacterized protein Espn isoform X1
XM_011185145.2	XP_011183447.1	uncharacterized protein Espn isoform X5
XM_011185141.1	XP_011183443.1	uncharacterized protein LOC105212834 isoform X2
XM_011185143.1	XP_011183445.1	uncharacterized protein LOC105212834 isoform X3
XM_011185144.2	XP_011183446.1	uncharacterized protein Espn isoform X4
XM_011185146.1	XP_011183448.1	uncharacterized protein Espn isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa199150	XM_011185140.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa199150	XM_029040633.1 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa199150	XM_011185139.2 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa199151	XM_011185141.2 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa199152	XM_011185143.2 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa199152	XM_011185142.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa199153	XM_011185144.2 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa199154	XM_011185145.2 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa199155	XM_011185146.1 Latest version!	Zeugodacus cucurbitae Espin (Espn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBa199150	XM_011185139.1	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa199154	XM_011185145.1	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa199151	XM_011185141.1	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa199152	XM_011185143.1	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa199153	XM_011185144.1	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

Espn ( XM_011185139.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183441.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa199150
Clone ID Related Accession (Same CDS sequence)	XM_011185139.1 , XM_011185140.1 , XM_011185139.2 , XM_029040633.1
Accession Version	XM_011185139.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183441.1
CDS	195..4259
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185139.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185139.1

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185140.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183442.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa199150
Clone ID Related Accession (Same CDS sequence)	XM_011185139.1 , XM_011185140.1 , XM_011185139.2 , XM_029040633.1
Accession Version	XM_011185140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183442.1
CDS	191..4255
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185140.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185140.1

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185139.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183441.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X1, mRNA.

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CloneID	OBa199150
Clone ID Related Accession (Same CDS sequence)	XM_011185139.1 , XM_011185140.1 , XM_011185139.2 , XM_029040633.1
Accession Version	XM_011185139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183441.1
CDS	422..4486
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185139.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185139.2

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_029040633.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028896466.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X2, mRNA.

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CloneID	OBa199150
Clone ID Related Accession (Same CDS sequence)	XM_011185139.1 , XM_011185140.1 , XM_011185139.2 , XM_029040633.1
Accession Version	XM_029040633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028896466.1
CDS	270..4334
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_029040633.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029040633.1

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGCTTTGTA TGCAGATTCC AGAAAAAGAT CAGGCAGGTA ACATCATTCC GGATTGGAAA 
CGTCAGATGA TGGCGAAAAA GGCAGCCGAA CGTGCCAAAA AAGAATTCGA GGAACGAATG 
GCAAAAGAAG CAGAAAATAG ACGCCTGTCA CAAATACCTC AATGGAAACG TGACCTACTA 
GCAAGGCGGG AGGAAACCGA AAATAAATTA AAAGCATCAA TTTATACACC CAAGGTGGAG 
GAAAATAATC GTATAGCTGA AACTTGGCAT CTTAAGAATC GAGCAATTTC AATTGATAAC 
ATTAATTTAG TTTCCCCGAG CGTTGATTCA CAGCTACTTG CTTCGGGAAC TTTTGACAGT 
AACAAAGAAA ATAAGGATCA AAATGAACAA GCCCTAATAA GTAACATTCT AACAACGGAT 
GAAGACATCA ATAGTAATCA ATATAGTAAA GGAGATGACG CCGATAATAT AATACCATGG 
CGGGCCCAGT TACGAAAAAC TAATAGTAGA CTAAGTTTAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185141.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183443.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X3, mRNA.

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CloneID	OBa199151
Clone ID Related Accession (Same CDS sequence)	XM_011185141.2 , XM_011185141.1
Accession Version	XM_011185141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGATTCCAG AAAAAGATCA GGCAGGTAAC ATCATTCCGG ATTGGAAACG TCAGATGATG 
GCGAAAAAGG CAGCCGAACG TGCCAAAAAA GAATTCGAGG AACGAATGGC AAAAGAAGCA 
GAAAATAGAC GCCTGTCACA AATACCTCAA TGGAAACGTG ACCTACTAGC AAGGCGGGAG 
GAAACCGAAA ATAAATTAAA AGCATCAATT TATACACCCA AGGTGGAGGA AAATAATCGT 
ATAGCTGAAA CTTGGCATCT TAAGAATCGA GCAATTTCAA TTGATAACAT TAATTTAGTT 
TCCCCGAGCG TTGATTCACA GCTACTTGCT TCGGGAACTT TTGACAGTAA CAAAGAAAAT 
AAGGATCAAA ATGAACAAGC CCTAATAAGT AACATTCTAA CAACGGATGA AGACATCAAT 
AGTAATCAAT ATAGTAAAGG AGATGACGCC GATAATATAA TACCATGGCG GGCCCAGTTA 
CGAAAAACTA ATAGTAGACT AAGTTTAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183443.1
CDS	423..4475
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185141.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185141.2

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGATTCCAG AAAAAGATCA GGCAGGTAAC ATCATTCCGG ATTGGAAACG TCAGATGATG 
GCGAAAAAGG CAGCCGAACG TGCCAAAAAA GAATTCGAGG AACGAATGGC AAAAGAAGCA 
GAAAATAGAC GCCTGTCACA AATACCTCAA TGGAAACGTG ACCTACTAGC AAGGCGGGAG 
GAAACCGAAA ATAAATTAAA AGCATCAATT TATACACCCA AGGTGGAGGA AAATAATCGT 
ATAGCTGAAA CTTGGCATCT TAAGAATCGA GCAATTTCAA TTGATAACAT TAATTTAGTT 
TCCCCGAGCG TTGATTCACA GCTACTTGCT TCGGGAACTT TTGACAGTAA CAAAGAAAAT 
AAGGATCAAA ATGAACAAGC CCTAATAAGT AACATTCTAA CAACGGATGA AGACATCAAT 
AGTAATCAAT ATAGTAAAGG AGATGACGCC GATAATATAA TACCATGGCG GGCCCAGTTA 
CGAAAAACTA ATAGTAGACT AAGTTTAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185141.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183443.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa199151
Clone ID Related Accession (Same CDS sequence)	XM_011185141.2 , XM_011185141.1
Accession Version	XM_011185141.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGATTCCAG AAAAAGATCA GGCAGGTAAC ATCATTCCGG ATTGGAAACG TCAGATGATG 
GCGAAAAAGG CAGCCGAACG TGCCAAAAAA GAATTCGAGG AACGAATGGC AAAAGAAGCA 
GAAAATAGAC GCCTGTCACA AATACCTCAA TGGAAACGTG ACCTACTAGC AAGGCGGGAG 
GAAACCGAAA ATAAATTAAA AGCATCAATT TATACACCCA AGGTGGAGGA AAATAATCGT 
ATAGCTGAAA CTTGGCATCT TAAGAATCGA GCAATTTCAA TTGATAACAT TAATTTAGTT 
TCCCCGAGCG TTGATTCACA GCTACTTGCT TCGGGAACTT TTGACAGTAA CAAAGAAAAT 
AAGGATCAAA ATGAACAAGC CCTAATAAGT AACATTCTAA CAACGGATGA AGACATCAAT 
AGTAATCAAT ATAGTAAAGG AGATGACGCC GATAATATAA TACCATGGCG GGCCCAGTTA 
CGAAAAACTA ATAGTAGACT AAGTTTAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183443.1
CDS	195..4247
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185141.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185141.1

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGTTACTTCT CTACAATCAG TGGTAGAAAA 
GGCTTTTTCG GTAGGATGAC CACTAGTTTC ACTTCCTATC GTCGTAGCAA AATAAAAAGT 
AAATCAAACA ACGACATTTC TTCTATTGTT GCTGAGATGA ATGGTGTTAG TGGTATAGTA 
GATAGCTTAC CGCAACACAT ATCTAACGAT GATATCTCAG ATTTAGCTGA AATTATGGAC 
AAAACTGGGC GAAAAGAGAG TAAACTCTGC AATGAAGACG TAGCTGTTGT ACGCAAAGAA 
CATAACAATC GGCGCAGCGT TGTAAGCGCG AACACTCAAA TGGACAACGA TGTCACACCG 
GTCTACTTAG CAGCGCAAGA AGGGCACTTG GAGGTGCTAA AATTTTTAGT TCTTGAAGCT 
GGCGGTTCTC TCTATGTGCG AGCACGCGAC GGAATGGCAC CGATACATGC TGCTTCGCAA 
ATGGGATGTT TGGATTGCCT TAGATGGATG GTGCAAGATC AGGGTGTTGA TCCAAATCTA 
CGAGATGGCG ATGGGGCTAC ACCGCTGCAC TTTGCCGCGT CAAGAGGACA CCTTTCTGTT 
GTACGGTGGT TACTCAAACA TGGTGCGAAA CTATCATTAG ACAAGTATGG CAAGTCCCCA 
ATTAACGATG CTGCAGAAAA CCAACAAGTA GAGTGTCTTA ATGTGTTAGT GCAACATGGT 
AGTACAATAG ATTATGCTTG TCGTGACAAC ATCCCACTAC AACGACAGAA AGCATGCTCC 
TTGTCTAATA AGGAATTTCG AGACATACAC AATTGCAATT TCAATAGTAC TGAAGGAGGT 
AACTGTATCA ATAGCAGCTC ACACGGGAGC TGTACCTCGA ATAGTAGTAA ACAGACTAGT 
TGCACCAACA CCATCAAATC AAAAACTTCT TCGACTTTAA GCTCAGATAT TGAGCCCTTT 
TACTTACATC CCCCATCGAT CTCTATGGCC CATAAACGCA TTGATGCCAT TTATGCACAT 
TCTCAACAAT CACGGTCATC GTCTGAGAAA TTATACAATG GACAAATGGT GCCGAATGAT 
GGCTTGTATA TAAATCCAAT GCGTAACAGT TTCTTTTCTC CCCCTTCCCC GACTGGCAGT 
GTTTCTGGAG AATCGTTTTT CCTCCACGAC CCTCATGAGC TAATTTATAA TCGAGTTCGG 
GAGTTGTTCG ATTCCGATTG TACCAGTATT GAAAATCAAA AACCGAAAAT GAATCGCCAC 
AAATTTCACA GTCATAGTAA AATGCATAAA TCAAATACCA TAAACAATGC AGTTGTTGTA 
CAAGCGGATG TGCACTCCAG TTCAAGTGGG ACAGGCAGCG GATCAGAAGA ATCTATTTCC 
GCTTCTATTC AATCAAGCAT GAATTTATCC AAAACAAACA ATCTTCGAAG TCAAAGTTTT 
AATCTTCACA CTAAATGTAA CAATGAAATT AGTAGCCACG ATCAAGGCTT CAACGCCAGT 
TCGAGTACAA AGACCATCAC CAATGATAAT AAAATAATTA ATGCAAATTT AACCTCTGGA 
ATCAACTACA ATAAAATCAA CAACAATAAT ATTAAAATTG ATAAACAAAA ACAAACAGCT 
AAGCAATCCA AAAGTAATTA TTTAAACAAT GAGAAGAAGA AACCAGAAAG TTCACATGAC 
CATGATTACG AGGATATATA TTTGGTTCGT GAGGAAGCGC GGAAAAACAA AAACAAATAC 
GGTTCCGGGC GATCACGGTC CCGTGATAGT GGGTCGCATT CACGTTCAGC TAGCACTTCC 
TCAACTCGAA GCTCAGACTT CGTACTACAA TATAGCAATC ATAATATGAA CAACAAACAT 
CAAAGTGAAG CACTGAATCG AAATAAATCG CAATCGACTA TTGGAATACA TAGCAAACCC 
AAATACGAGT TATCGCAAAA GGAAAATTTC ACCGTTAAAA ATGTGAATCT AAAGAACCTG 
CTGAACGTCA ATAGCAATAC CAATGGTGGT GTTAAAAGCG ACACCTATGA AAGCGTGTGT 
CCGATAGAAG ATATTGCTGA GCGCACAAAG CAATCACAGA AAAACTCCGT TATTCGAAAT 
AATCTTGATT GTGGTAGCAT TGGTAACACC AGCAATATAT TCCACCCAAT ATGCAATGAT 
GTCAGCACAT GCGATCGCCA GTTGAAACGA GTTTCTTCTG CTCCGCCTAT ACAAAACATA 
GAAATCGTAA GTGGACCGCC ACCACCTCCA CTGCCGCCAC CGATGAACGC TCCCTTACTC 
AAGAGAATGC AATTAGTTGC ATCAGATCCC ATGAACTTTT GCATTAGTCA CTCAAATAGC 
AACTTGGATA ATATAGATTC AGATTCAGGG CTTGAGGTAA TTGAGGAACC TAGTTTACGT 
CCGTCTGAAT TGATCAGAAG CAAACACAAC CGAACGATGT CTACCATATC AGCAAACAAG 
AAAGCAAAAC TTCTTAATGG CGGATGTAAT GGAAATATAC CCAACTCTGC TCCAATTGCC 
AATTGTGTTA CACCAAATAG AGAAAACAGT AATTATCATG ATAAAAGCTT TGCTTGTGAT 
GGTTATCACG TTTGCAATGA TCAACAAGAT GATAGAGTTC AGGTGCGCAA TTACCATTCA 
CACCATATAG ATGTCCAACA CAGCTATCAG CAATCGCAAA TGTATAGATC TTCTGCCGAA 
GATCACTACG AACTACAACA GGCACAGTAT GGGTACAGCA ATTCTAATGG GAGCACAGTA 
TGTGGCCCAC GTACCGGTGG TCCAAACTTA GTTAATAAAC AGTTAGTACT TCCCTTCGTG 
CCACCCAGTT TTCCAAACAA ATCACAAGAT GGCGTAACAC ATCTTATAAA GCCATCTGAA 
TATCTAAAGA GTATAAGTGA TAAGCGATCC TGCCCGTCTT CAGCAAGATC CACAGATACT 
GAAGATTACA TGCATATCCA GGTGGCTAAT CAACATGGAG TTAATTGTGA ACCACCAAAG 
CCTCCACCGC CGCCACCACT CCCAATTCAT CCGTTAATGC ATCACAATGA AAAACAAAAT 
AGTGTAAAAA CAGGAAATAT TCAGCATGTA TCTGTAGTTA ATCAGGATAC CGCCACCAGG 
AAGCAGCATC AACCTCTCTC CGCCATCTCC ATTCAAGATC TTAATAGTGT TCAGCTTCGA 
CGTACTGATA CTCAAAAAAT GCCTAAGCCT TACCAAATGC CTGCACGCAG TTTAAGTATG 
CAGTGTCTTA CCACGTCAGC TGATTCTTAC CTTAAAACGG ATTTAATTGC TGAATTAAAG 
ATTTCAAAAG ACATTACGGG CATTAAGAAA ATGAAAGTGG AAAAACAGTT GGCCAGTCAA 
TTTGACACTG AACATTATTC AGAAATTTCC AAACAATTTT CGACAAACAA CTATATGGAA 
CAGATTCCAG AAAAAGATCA GGCAGGTAAC ATCATTCCGG ATTGGAAACG TCAGATGATG 
GCGAAAAAGG CAGCCGAACG TGCCAAAAAA GAATTCGAGG AACGAATGGC AAAAGAAGCA 
GAAAATAGAC GCCTGTCACA AATACCTCAA TGGAAACGTG ACCTACTAGC AAGGCGGGAG 
GAAACCGAAA ATAAATTAAA AGCATCAATT TATACACCCA AGGTGGAGGA AAATAATCGT 
ATAGCTGAAA CTTGGCATCT TAAGAATCGA GCAATTTCAA TTGATAACAT TAATTTAGTT 
TCCCCGAGCG TTGATTCACA GCTACTTGCT TCGGGAACTT TTGACAGTAA CAAAGAAAAT 
AAGGATCAAA ATGAACAAGC CCTAATAAGT AACATTCTAA CAACGGATGA AGACATCAAT 
AGTAATCAAT ATAGTAAAGG AGATGACGCC GATAATATAA TACCATGGCG GGCCCAGTTA 
CGAAAAACTA ATAGTAGACT AAGTTTAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185142.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183444.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X4, mRNA.

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CloneID	OBa199152
Clone ID Related Accession (Same CDS sequence)	XM_011185142.1 , XM_011185143.2 , XM_011185143.1
Accession Version	XM_011185142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGTT ACTTCTCTAC AATCAGTGGT AGAAAAGGCT TTTTCGGTAG GATGACCACT 
AGTTTCACTT CCTATCGTCG TAGCAAAATA AAAAGTAAAT CAAACAACGA CATTTCTTCT 
ATTGTTGCTG AGATGAATGG TGTTAGTGGT ATAGTAGATA GCTTACCGCA ACACATATCT 
AACGATGATA TCTCAGATTT AGCTGAAATT ATGGACAAAA CTGGGCGAAA AGAGAGTAAA 
CTCTGCAATG AAGACGTAGC TGTTGTACGC AAAGAACATA ACAATCGGCG CAGCGTTGTA 
AGCGCGAACA CTCAAATGGA CAACGATGTC ACACCGGTCT ACTTAGCAGC GCAAGAAGGG 
CACTTGGAGG TGCTAAAATT TTTAGTTCTT GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA 
CGCGACGGAA TGGCACCGAT ACATGCTGCT TCGCAAATGG GATGTTTGGA TTGCCTTAGA 
TGGATGGTGC AAGATCAGGG TGTTGATCCA AATCTACGAG ATGGCGATGG GGCTACACCG 
CTGCACTTTG CCGCGTCAAG AGGACACCTT TCTGTTGTAC GGTGGTTACT CAAACATGGT 
GCGAAACTAT CATTAGACAA GTATGGCAAG TCCCCAATTA ACGATGCTGC AGAAAACCAA 
CAAGTAGAGT GTCTTAATGT GTTAGTGCAA CATGGTAGTA CAATAGATTA TGCTTGTCGT 
GACAACATCC CACTACAACG ACAGAAAGCA TGCTCCTTGT CTAATAAGGA ATTTCGAGAC 
ATACACAATT GCAATTTCAA TAGTACTGAA GGAGGTAACT GTATCAATAG CAGCTCACAC 
GGGAGCTGTA CCTCGAATAG TAGTAAACAG ACTAGTTGCA CCAACACCAT CAAATCAAAA 
ACTTCTTCGA CTTTAAGCTC AGATATTGAG CCCTTTTACT TACATCCCCC ATCGATCTCT 
ATGGCCCATA AACGCATTGA TGCCATTTAT GCACATTCTC AACAATCACG GTCATCGTCT 
GAGAAATTAT ACAATGGACA AATGGTGCCG AATGATGGCT TGTATATAAA TCCAATGCGT 
AACAGTTTCT TTTCTCCCCC TTCCCCGACT GGCAGTGTTT CTGGAGAATC GTTTTTCCTC 
CACGACCCTC ATGAGCTAAT TTATAATCGA GTTCGGGAGT TGTTCGATTC CGATTGTACC 
AGTATTGAAA ATCAAAAACC GAAAATGAAT CGCCACAAAT TTCACAGTCA TAGTAAAATG 
CATAAATCAA ATACCATAAA CAATGCAGTT GTTGTACAAG CGGATGTGCA CTCCAGTTCA 
AGTGGGACAG GCAGCGGATC AGAAGAATCT ATTTCCGCTT CTATTCAATC AAGCATGAAT 
TTATCCAAAA CAAACAATCT TCGAAGTCAA AGTTTTAATC TTCACACTAA ATGTAACAAT 
GAAATTAGTA GCCACGATCA AGGCTTCAAC GCCAGTTCGA GTACAAAGAC CATCACCAAT 
GATAATAAAA TAATTAATGC AAATTTAACC TCTGGAATCA ACTACAATAA AATCAACAAC 
AATAATATTA AAATTGATAA ACAAAAACAA ACAGCTAAGC AATCCAAAAG TAATTATTTA 
AACAATGAGA AGAAGAAACC AGAAAGTTCA CATGACCATG ATTACGAGGA TATATATTTG 
GTTCGTGAGG AAGCGCGGAA AAACAAAAAC AAATACGGTT CCGGGCGATC ACGGTCCCGT 
GATAGTGGGT CGCATTCACG TTCAGCTAGC ACTTCCTCAA CTCGAAGCTC AGACTTCGTA 
CTACAATATA GCAATCATAA TATGAACAAC AAACATCAAA GTGAAGCACT GAATCGAAAT 
AAATCGCAAT CGACTATTGG AATACATAGC AAACCCAAAT ACGAGTTATC GCAAAAGGAA 
AATTTCACCG TTAAAAATGT GAATCTAAAG AACCTGCTGA ACGTCAATAG CAATACCAAT 
GGTGGTGTTA AAAGCGACAC CTATGAAAGC GTGTGTCCGA TAGAAGATAT TGCTGAGCGC 
ACAAAGCAAT CACAGAAAAA CTCCGTTATT CGAAATAATC TTGATTGTGG TAGCATTGGT 
AACACCAGCA ATATATTCCA CCCAATATGC AATGATGTCA GCACATGCGA TCGCCAGTTG 
AAACGAGTTT CTTCTGCTCC GCCTATACAA AACATAGAAA TCGTAAGTGG ACCGCCACCA 
CCTCCACTGC CGCCACCGAT GAACGCTCCC TTACTCAAGA GAATGCAATT AGTTGCATCA 
GATCCCATGA ACTTTTGCAT TAGTCACTCA AATAGCAACT TGGATAATAT AGATTCAGAT 
TCAGGGCTTG AGGTAATTGA GGAACCTAGT TTACGTCCGT CTGAATTGAT CAGAAGCAAA 
CACAACCGAA CGATGTCTAC CATATCAGCA AACAAGAAAG CAAAACTTCT TAATGGCGGA 
TGTAATGGAA ATATACCCAA CTCTGCTCCA ATTGCCAATT GTGTTACACC AAATAGAGAA 
AACAGTAATT ATCATGATAA AAGCTTTGCT TGTGATGGTT ATCACGTTTG CAATGATCAA 
CAAGATGATA GAGTTCAGGT GCGCAATTAC CATTCACACC ATATAGATGT CCAACACAGC 
TATCAGCAAT CGCAAATGTA TAGATCTTCT GCCGAAGATC ACTACGAACT ACAACAGGCA 
CAGTATGGGT ACAGCAATTC TAATGGGAGC ACAGTATGTG GCCCACGTAC CGGTGGTCCA 
AACTTAGTTA ATAAACAGTT AGTACTTCCC TTCGTGCCAC CCAGTTTTCC AAACAAATCA 
CAAGATGGCG TAACACATCT TATAAAGCCA TCTGAATATC TAAAGAGTAT AAGTGATAAG 
CGATCCTGCC CGTCTTCAGC AAGATCCACA GATACTGAAG ATTACATGCA TATCCAGGTG 
GCTAATCAAC ATGGAGTTAA TTGTGAACCA CCAAAGCCTC CACCGCCGCC ACCACTCCCA 
ATTCATCCGT TAATGCATCA CAATGAAAAA CAAAATAGTG TAAAAACAGG AAATATTCAG 
CATGTATCTG TAGTTAATCA GGATACCGCC ACCAGGAAGC AGCATCAACC TCTCTCCGCC 
ATCTCCATTC AAGATCTTAA TAGTGTTCAG CTTCGACGTA CTGATACTCA AAAAATGCCT 
AAGCCTTACC AAATGCCTGC ACGCAGTTTA AGTATGCAGT GTCTTACCAC GTCAGCTGAT 
TCTTACCTTA AAACGGATTT AATTGCTGAA TTAAAGATTT CAAAAGACAT TACGGGCATT 
AAGAAAATGA AAGTGGAAAA ACAGTTGGCC AGTCAATTTG ACACTGAACA TTATTCAGAA 
ATTTCCAAAC AATTTTCGAC AAACAACTAT ATGGAACAGC TTTGTATGCA GATTCCAGAA 
AAAGATCAGG CAGGTAACAT CATTCCGGAT TGGAAACGTC AGATGATGGC GAAAAAGGCA 
GCCGAACGTG CCAAAAAAGA ATTCGAGGAA CGAATGGCAA AAGAAGCAGA AAATAGACGC 
CTGTCACAAA TACCTCAATG GAAACGTGAC CTACTAGCAA GGCGGGAGGA AACCGAAAAT 
AAATTAAAAG CATCAATTTA TACACCCAAG GTGGAGGAAA ATAATCGTAT AGCTGAAACT 
TGGCATCTTA AGAATCGAGC AATTTCAATT GATAACATTA ATTTAGTTTC CCCGAGCGTT 
GATTCACAGC TACTTGCTTC GGGAACTTTT GACAGTAACA AAGAAAATAA GGATCAAAAT 
GAACAAGCCC TAATAAGTAA CATTCTAACA ACGGATGAAG ACATCAATAG TAATCAATAT 
AGTAAAGGAG ATGACGCCGA TAATATAATA CCATGGCGGG CCCAGTTACG AAAAACTAAT 
AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183444.1
CDS	573..4493
Translation	MKSYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185142.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185142.1

ORF Insert Sequence:

ATGAAAAGTT ACTTCTCTAC AATCAGTGGT AGAAAAGGCT TTTTCGGTAG GATGACCACT 
AGTTTCACTT CCTATCGTCG TAGCAAAATA AAAAGTAAAT CAAACAACGA CATTTCTTCT 
ATTGTTGCTG AGATGAATGG TGTTAGTGGT ATAGTAGATA GCTTACCGCA ACACATATCT 
AACGATGATA TCTCAGATTT AGCTGAAATT ATGGACAAAA CTGGGCGAAA AGAGAGTAAA 
CTCTGCAATG AAGACGTAGC TGTTGTACGC AAAGAACATA ACAATCGGCG CAGCGTTGTA 
AGCGCGAACA CTCAAATGGA CAACGATGTC ACACCGGTCT ACTTAGCAGC GCAAGAAGGG 
CACTTGGAGG TGCTAAAATT TTTAGTTCTT GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA 
CGCGACGGAA TGGCACCGAT ACATGCTGCT TCGCAAATGG GATGTTTGGA TTGCCTTAGA 
TGGATGGTGC AAGATCAGGG TGTTGATCCA AATCTACGAG ATGGCGATGG GGCTACACCG 
CTGCACTTTG CCGCGTCAAG AGGACACCTT TCTGTTGTAC GGTGGTTACT CAAACATGGT 
GCGAAACTAT CATTAGACAA GTATGGCAAG TCCCCAATTA ACGATGCTGC AGAAAACCAA 
CAAGTAGAGT GTCTTAATGT GTTAGTGCAA CATGGTAGTA CAATAGATTA TGCTTGTCGT 
GACAACATCC CACTACAACG ACAGAAAGCA TGCTCCTTGT CTAATAAGGA ATTTCGAGAC 
ATACACAATT GCAATTTCAA TAGTACTGAA GGAGGTAACT GTATCAATAG CAGCTCACAC 
GGGAGCTGTA CCTCGAATAG TAGTAAACAG ACTAGTTGCA CCAACACCAT CAAATCAAAA 
ACTTCTTCGA CTTTAAGCTC AGATATTGAG CCCTTTTACT TACATCCCCC ATCGATCTCT 
ATGGCCCATA AACGCATTGA TGCCATTTAT GCACATTCTC AACAATCACG GTCATCGTCT 
GAGAAATTAT ACAATGGACA AATGGTGCCG AATGATGGCT TGTATATAAA TCCAATGCGT 
AACAGTTTCT TTTCTCCCCC TTCCCCGACT GGCAGTGTTT CTGGAGAATC GTTTTTCCTC 
CACGACCCTC ATGAGCTAAT TTATAATCGA GTTCGGGAGT TGTTCGATTC CGATTGTACC 
AGTATTGAAA ATCAAAAACC GAAAATGAAT CGCCACAAAT TTCACAGTCA TAGTAAAATG 
CATAAATCAA ATACCATAAA CAATGCAGTT GTTGTACAAG CGGATGTGCA CTCCAGTTCA 
AGTGGGACAG GCAGCGGATC AGAAGAATCT ATTTCCGCTT CTATTCAATC AAGCATGAAT 
TTATCCAAAA CAAACAATCT TCGAAGTCAA AGTTTTAATC TTCACACTAA ATGTAACAAT 
GAAATTAGTA GCCACGATCA AGGCTTCAAC GCCAGTTCGA GTACAAAGAC CATCACCAAT 
GATAATAAAA TAATTAATGC AAATTTAACC TCTGGAATCA ACTACAATAA AATCAACAAC 
AATAATATTA AAATTGATAA ACAAAAACAA ACAGCTAAGC AATCCAAAAG TAATTATTTA 
AACAATGAGA AGAAGAAACC AGAAAGTTCA CATGACCATG ATTACGAGGA TATATATTTG 
GTTCGTGAGG AAGCGCGGAA AAACAAAAAC AAATACGGTT CCGGGCGATC ACGGTCCCGT 
GATAGTGGGT CGCATTCACG TTCAGCTAGC ACTTCCTCAA CTCGAAGCTC AGACTTCGTA 
CTACAATATA GCAATCATAA TATGAACAAC AAACATCAAA GTGAAGCACT GAATCGAAAT 
AAATCGCAAT CGACTATTGG AATACATAGC AAACCCAAAT ACGAGTTATC GCAAAAGGAA 
AATTTCACCG TTAAAAATGT GAATCTAAAG AACCTGCTGA ACGTCAATAG CAATACCAAT 
GGTGGTGTTA AAAGCGACAC CTATGAAAGC GTGTGTCCGA TAGAAGATAT TGCTGAGCGC 
ACAAAGCAAT CACAGAAAAA CTCCGTTATT CGAAATAATC TTGATTGTGG TAGCATTGGT 
AACACCAGCA ATATATTCCA CCCAATATGC AATGATGTCA GCACATGCGA TCGCCAGTTG 
AAACGAGTTT CTTCTGCTCC GCCTATACAA AACATAGAAA TCGTAAGTGG ACCGCCACCA 
CCTCCACTGC CGCCACCGAT GAACGCTCCC TTACTCAAGA GAATGCAATT AGTTGCATCA 
GATCCCATGA ACTTTTGCAT TAGTCACTCA AATAGCAACT TGGATAATAT AGATTCAGAT 
TCAGGGCTTG AGGTAATTGA GGAACCTAGT TTACGTCCGT CTGAATTGAT CAGAAGCAAA 
CACAACCGAA CGATGTCTAC CATATCAGCA AACAAGAAAG CAAAACTTCT TAATGGCGGA 
TGTAATGGAA ATATACCCAA CTCTGCTCCA ATTGCCAATT GTGTTACACC AAATAGAGAA 
AACAGTAATT ATCATGATAA AAGCTTTGCT TGTGATGGTT ATCACGTTTG CAATGATCAA 
CAAGATGATA GAGTTCAGGT GCGCAATTAC CATTCACACC ATATAGATGT CCAACACAGC 
TATCAGCAAT CGCAAATGTA TAGATCTTCT GCCGAAGATC ACTACGAACT ACAACAGGCA 
CAGTATGGGT ACAGCAATTC TAATGGGAGC ACAGTATGTG GCCCACGTAC CGGTGGTCCA 
AACTTAGTTA ATAAACAGTT AGTACTTCCC TTCGTGCCAC CCAGTTTTCC AAACAAATCA 
CAAGATGGCG TAACACATCT TATAAAGCCA TCTGAATATC TAAAGAGTAT AAGTGATAAG 
CGATCCTGCC CGTCTTCAGC AAGATCCACA GATACTGAAG ATTACATGCA TATCCAGGTG 
GCTAATCAAC ATGGAGTTAA TTGTGAACCA CCAAAGCCTC CACCGCCGCC ACCACTCCCA 
ATTCATCCGT TAATGCATCA CAATGAAAAA CAAAATAGTG TAAAAACAGG AAATATTCAG 
CATGTATCTG TAGTTAATCA GGATACCGCC ACCAGGAAGC AGCATCAACC TCTCTCCGCC 
ATCTCCATTC AAGATCTTAA TAGTGTTCAG CTTCGACGTA CTGATACTCA AAAAATGCCT 
AAGCCTTACC AAATGCCTGC ACGCAGTTTA AGTATGCAGT GTCTTACCAC GTCAGCTGAT 
TCTTACCTTA AAACGGATTT AATTGCTGAA TTAAAGATTT CAAAAGACAT TACGGGCATT 
AAGAAAATGA AAGTGGAAAA ACAGTTGGCC AGTCAATTTG ACACTGAACA TTATTCAGAA 
ATTTCCAAAC AATTTTCGAC AAACAACTAT ATGGAACAGC TTTGTATGCA GATTCCAGAA 
AAAGATCAGG CAGGTAACAT CATTCCGGAT TGGAAACGTC AGATGATGGC GAAAAAGGCA 
GCCGAACGTG CCAAAAAAGA ATTCGAGGAA CGAATGGCAA AAGAAGCAGA AAATAGACGC 
CTGTCACAAA TACCTCAATG GAAACGTGAC CTACTAGCAA GGCGGGAGGA AACCGAAAAT 
AAATTAAAAG CATCAATTTA TACACCCAAG GTGGAGGAAA ATAATCGTAT AGCTGAAACT 
TGGCATCTTA AGAATCGAGC AATTTCAATT GATAACATTA ATTTAGTTTC CCCGAGCGTT 
GATTCACAGC TACTTGCTTC GGGAACTTTT GACAGTAACA AAGAAAATAA GGATCAAAAT 
GAACAAGCCC TAATAAGTAA CATTCTAACA ACGGATGAAG ACATCAATAG TAATCAATAT 
AGTAAAGGAG ATGACGCCGA TAATATAATA CCATGGCGGG CCCAGTTACG AAAAACTAAT 
AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185143.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183445.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa199152
Clone ID Related Accession (Same CDS sequence)	XM_011185142.1 , XM_011185143.2 , XM_011185143.1
Accession Version	XM_011185143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGTT ACTTCTCTAC AATCAGTGGT AGAAAAGGCT TTTTCGGTAG GATGACCACT 
AGTTTCACTT CCTATCGTCG TAGCAAAATA AAAAGTAAAT CAAACAACGA CATTTCTTCT 
ATTGTTGCTG AGATGAATGG TGTTAGTGGT ATAGTAGATA GCTTACCGCA ACACATATCT 
AACGATGATA TCTCAGATTT AGCTGAAATT ATGGACAAAA CTGGGCGAAA AGAGAGTAAA 
CTCTGCAATG AAGACGTAGC TGTTGTACGC AAAGAACATA ACAATCGGCG CAGCGTTGTA 
AGCGCGAACA CTCAAATGGA CAACGATGTC ACACCGGTCT ACTTAGCAGC GCAAGAAGGG 
CACTTGGAGG TGCTAAAATT TTTAGTTCTT GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA 
CGCGACGGAA TGGCACCGAT ACATGCTGCT TCGCAAATGG GATGTTTGGA TTGCCTTAGA 
TGGATGGTGC AAGATCAGGG TGTTGATCCA AATCTACGAG ATGGCGATGG GGCTACACCG 
CTGCACTTTG CCGCGTCAAG AGGACACCTT TCTGTTGTAC GGTGGTTACT CAAACATGGT 
GCGAAACTAT CATTAGACAA GTATGGCAAG TCCCCAATTA ACGATGCTGC AGAAAACCAA 
CAAGTAGAGT GTCTTAATGT GTTAGTGCAA CATGGTAGTA CAATAGATTA TGCTTGTCGT 
GACAACATCC CACTACAACG ACAGAAAGCA TGCTCCTTGT CTAATAAGGA ATTTCGAGAC 
ATACACAATT GCAATTTCAA TAGTACTGAA GGAGGTAACT GTATCAATAG CAGCTCACAC 
GGGAGCTGTA CCTCGAATAG TAGTAAACAG ACTAGTTGCA CCAACACCAT CAAATCAAAA 
ACTTCTTCGA CTTTAAGCTC AGATATTGAG CCCTTTTACT TACATCCCCC ATCGATCTCT 
ATGGCCCATA AACGCATTGA TGCCATTTAT GCACATTCTC AACAATCACG GTCATCGTCT 
GAGAAATTAT ACAATGGACA AATGGTGCCG AATGATGGCT TGTATATAAA TCCAATGCGT 
AACAGTTTCT TTTCTCCCCC TTCCCCGACT GGCAGTGTTT CTGGAGAATC GTTTTTCCTC 
CACGACCCTC ATGAGCTAAT TTATAATCGA GTTCGGGAGT TGTTCGATTC CGATTGTACC 
AGTATTGAAA ATCAAAAACC GAAAATGAAT CGCCACAAAT TTCACAGTCA TAGTAAAATG 
CATAAATCAA ATACCATAAA CAATGCAGTT GTTGTACAAG CGGATGTGCA CTCCAGTTCA 
AGTGGGACAG GCAGCGGATC AGAAGAATCT ATTTCCGCTT CTATTCAATC AAGCATGAAT 
TTATCCAAAA CAAACAATCT TCGAAGTCAA AGTTTTAATC TTCACACTAA ATGTAACAAT 
GAAATTAGTA GCCACGATCA AGGCTTCAAC GCCAGTTCGA GTACAAAGAC CATCACCAAT 
GATAATAAAA TAATTAATGC AAATTTAACC TCTGGAATCA ACTACAATAA AATCAACAAC 
AATAATATTA AAATTGATAA ACAAAAACAA ACAGCTAAGC AATCCAAAAG TAATTATTTA 
AACAATGAGA AGAAGAAACC AGAAAGTTCA CATGACCATG ATTACGAGGA TATATATTTG 
GTTCGTGAGG AAGCGCGGAA AAACAAAAAC AAATACGGTT CCGGGCGATC ACGGTCCCGT 
GATAGTGGGT CGCATTCACG TTCAGCTAGC ACTTCCTCAA CTCGAAGCTC AGACTTCGTA 
CTACAATATA GCAATCATAA TATGAACAAC AAACATCAAA GTGAAGCACT GAATCGAAAT 
AAATCGCAAT CGACTATTGG AATACATAGC AAACCCAAAT ACGAGTTATC GCAAAAGGAA 
AATTTCACCG TTAAAAATGT GAATCTAAAG AACCTGCTGA ACGTCAATAG CAATACCAAT 
GGTGGTGTTA AAAGCGACAC CTATGAAAGC GTGTGTCCGA TAGAAGATAT TGCTGAGCGC 
ACAAAGCAAT CACAGAAAAA CTCCGTTATT CGAAATAATC TTGATTGTGG TAGCATTGGT 
AACACCAGCA ATATATTCCA CCCAATATGC AATGATGTCA GCACATGCGA TCGCCAGTTG 
AAACGAGTTT CTTCTGCTCC GCCTATACAA AACATAGAAA TCGTAAGTGG ACCGCCACCA 
CCTCCACTGC CGCCACCGAT GAACGCTCCC TTACTCAAGA GAATGCAATT AGTTGCATCA 
GATCCCATGA ACTTTTGCAT TAGTCACTCA AATAGCAACT TGGATAATAT AGATTCAGAT 
TCAGGGCTTG AGGTAATTGA GGAACCTAGT TTACGTCCGT CTGAATTGAT CAGAAGCAAA 
CACAACCGAA CGATGTCTAC CATATCAGCA AACAAGAAAG CAAAACTTCT TAATGGCGGA 
TGTAATGGAA ATATACCCAA CTCTGCTCCA ATTGCCAATT GTGTTACACC AAATAGAGAA 
AACAGTAATT ATCATGATAA AAGCTTTGCT TGTGATGGTT ATCACGTTTG CAATGATCAA 
CAAGATGATA GAGTTCAGGT GCGCAATTAC CATTCACACC ATATAGATGT CCAACACAGC 
TATCAGCAAT CGCAAATGTA TAGATCTTCT GCCGAAGATC ACTACGAACT ACAACAGGCA 
CAGTATGGGT ACAGCAATTC TAATGGGAGC ACAGTATGTG GCCCACGTAC CGGTGGTCCA 
AACTTAGTTA ATAAACAGTT AGTACTTCCC TTCGTGCCAC CCAGTTTTCC AAACAAATCA 
CAAGATGGCG TAACACATCT TATAAAGCCA TCTGAATATC TAAAGAGTAT AAGTGATAAG 
CGATCCTGCC CGTCTTCAGC AAGATCCACA GATACTGAAG ATTACATGCA TATCCAGGTG 
GCTAATCAAC ATGGAGTTAA TTGTGAACCA CCAAAGCCTC CACCGCCGCC ACCACTCCCA 
ATTCATCCGT TAATGCATCA CAATGAAAAA CAAAATAGTG TAAAAACAGG AAATATTCAG 
CATGTATCTG TAGTTAATCA GGATACCGCC ACCAGGAAGC AGCATCAACC TCTCTCCGCC 
ATCTCCATTC AAGATCTTAA TAGTGTTCAG CTTCGACGTA CTGATACTCA AAAAATGCCT 
AAGCCTTACC AAATGCCTGC ACGCAGTTTA AGTATGCAGT GTCTTACCAC GTCAGCTGAT 
TCTTACCTTA AAACGGATTT AATTGCTGAA TTAAAGATTT CAAAAGACAT TACGGGCATT 
AAGAAAATGA AAGTGGAAAA ACAGTTGGCC AGTCAATTTG ACACTGAACA TTATTCAGAA 
ATTTCCAAAC AATTTTCGAC AAACAACTAT ATGGAACAGC TTTGTATGCA GATTCCAGAA 
AAAGATCAGG CAGGTAACAT CATTCCGGAT TGGAAACGTC AGATGATGGC GAAAAAGGCA 
GCCGAACGTG CCAAAAAAGA ATTCGAGGAA CGAATGGCAA AAGAAGCAGA AAATAGACGC 
CTGTCACAAA TACCTCAATG GAAACGTGAC CTACTAGCAA GGCGGGAGGA AACCGAAAAT 
AAATTAAAAG CATCAATTTA TACACCCAAG GTGGAGGAAA ATAATCGTAT AGCTGAAACT 
TGGCATCTTA AGAATCGAGC AATTTCAATT GATAACATTA ATTTAGTTTC CCCGAGCGTT 
GATTCACAGC TACTTGCTTC GGGAACTTTT GACAGTAACA AAGAAAATAA GGATCAAAAT 
GAACAAGCCC TAATAAGTAA CATTCTAACA ACGGATGAAG ACATCAATAG TAATCAATAT 
AGTAAAGGAG ATGACGCCGA TAATATAATA CCATGGCGGG CCCAGTTACG AAAAACTAAT 
AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183445.1
CDS	475..4395
Translation	MKSYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185143.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185143.2

ORF Insert Sequence:

ATGAAAAGTT ACTTCTCTAC AATCAGTGGT AGAAAAGGCT TTTTCGGTAG GATGACCACT 
AGTTTCACTT CCTATCGTCG TAGCAAAATA AAAAGTAAAT CAAACAACGA CATTTCTTCT 
ATTGTTGCTG AGATGAATGG TGTTAGTGGT ATAGTAGATA GCTTACCGCA ACACATATCT 
AACGATGATA TCTCAGATTT AGCTGAAATT ATGGACAAAA CTGGGCGAAA AGAGAGTAAA 
CTCTGCAATG AAGACGTAGC TGTTGTACGC AAAGAACATA ACAATCGGCG CAGCGTTGTA 
AGCGCGAACA CTCAAATGGA CAACGATGTC ACACCGGTCT ACTTAGCAGC GCAAGAAGGG 
CACTTGGAGG TGCTAAAATT TTTAGTTCTT GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA 
CGCGACGGAA TGGCACCGAT ACATGCTGCT TCGCAAATGG GATGTTTGGA TTGCCTTAGA 
TGGATGGTGC AAGATCAGGG TGTTGATCCA AATCTACGAG ATGGCGATGG GGCTACACCG 
CTGCACTTTG CCGCGTCAAG AGGACACCTT TCTGTTGTAC GGTGGTTACT CAAACATGGT 
GCGAAACTAT CATTAGACAA GTATGGCAAG TCCCCAATTA ACGATGCTGC AGAAAACCAA 
CAAGTAGAGT GTCTTAATGT GTTAGTGCAA CATGGTAGTA CAATAGATTA TGCTTGTCGT 
GACAACATCC CACTACAACG ACAGAAAGCA TGCTCCTTGT CTAATAAGGA ATTTCGAGAC 
ATACACAATT GCAATTTCAA TAGTACTGAA GGAGGTAACT GTATCAATAG CAGCTCACAC 
GGGAGCTGTA CCTCGAATAG TAGTAAACAG ACTAGTTGCA CCAACACCAT CAAATCAAAA 
ACTTCTTCGA CTTTAAGCTC AGATATTGAG CCCTTTTACT TACATCCCCC ATCGATCTCT 
ATGGCCCATA AACGCATTGA TGCCATTTAT GCACATTCTC AACAATCACG GTCATCGTCT 
GAGAAATTAT ACAATGGACA AATGGTGCCG AATGATGGCT TGTATATAAA TCCAATGCGT 
AACAGTTTCT TTTCTCCCCC TTCCCCGACT GGCAGTGTTT CTGGAGAATC GTTTTTCCTC 
CACGACCCTC ATGAGCTAAT TTATAATCGA GTTCGGGAGT TGTTCGATTC CGATTGTACC 
AGTATTGAAA ATCAAAAACC GAAAATGAAT CGCCACAAAT TTCACAGTCA TAGTAAAATG 
CATAAATCAA ATACCATAAA CAATGCAGTT GTTGTACAAG CGGATGTGCA CTCCAGTTCA 
AGTGGGACAG GCAGCGGATC AGAAGAATCT ATTTCCGCTT CTATTCAATC AAGCATGAAT 
TTATCCAAAA CAAACAATCT TCGAAGTCAA AGTTTTAATC TTCACACTAA ATGTAACAAT 
GAAATTAGTA GCCACGATCA AGGCTTCAAC GCCAGTTCGA GTACAAAGAC CATCACCAAT 
GATAATAAAA TAATTAATGC AAATTTAACC TCTGGAATCA ACTACAATAA AATCAACAAC 
AATAATATTA AAATTGATAA ACAAAAACAA ACAGCTAAGC AATCCAAAAG TAATTATTTA 
AACAATGAGA AGAAGAAACC AGAAAGTTCA CATGACCATG ATTACGAGGA TATATATTTG 
GTTCGTGAGG AAGCGCGGAA AAACAAAAAC AAATACGGTT CCGGGCGATC ACGGTCCCGT 
GATAGTGGGT CGCATTCACG TTCAGCTAGC ACTTCCTCAA CTCGAAGCTC AGACTTCGTA 
CTACAATATA GCAATCATAA TATGAACAAC AAACATCAAA GTGAAGCACT GAATCGAAAT 
AAATCGCAAT CGACTATTGG AATACATAGC AAACCCAAAT ACGAGTTATC GCAAAAGGAA 
AATTTCACCG TTAAAAATGT GAATCTAAAG AACCTGCTGA ACGTCAATAG CAATACCAAT 
GGTGGTGTTA AAAGCGACAC CTATGAAAGC GTGTGTCCGA TAGAAGATAT TGCTGAGCGC 
ACAAAGCAAT CACAGAAAAA CTCCGTTATT CGAAATAATC TTGATTGTGG TAGCATTGGT 
AACACCAGCA ATATATTCCA CCCAATATGC AATGATGTCA GCACATGCGA TCGCCAGTTG 
AAACGAGTTT CTTCTGCTCC GCCTATACAA AACATAGAAA TCGTAAGTGG ACCGCCACCA 
CCTCCACTGC CGCCACCGAT GAACGCTCCC TTACTCAAGA GAATGCAATT AGTTGCATCA 
GATCCCATGA ACTTTTGCAT TAGTCACTCA AATAGCAACT TGGATAATAT AGATTCAGAT 
TCAGGGCTTG AGGTAATTGA GGAACCTAGT TTACGTCCGT CTGAATTGAT CAGAAGCAAA 
CACAACCGAA CGATGTCTAC CATATCAGCA AACAAGAAAG CAAAACTTCT TAATGGCGGA 
TGTAATGGAA ATATACCCAA CTCTGCTCCA ATTGCCAATT GTGTTACACC AAATAGAGAA 
AACAGTAATT ATCATGATAA AAGCTTTGCT TGTGATGGTT ATCACGTTTG CAATGATCAA 
CAAGATGATA GAGTTCAGGT GCGCAATTAC CATTCACACC ATATAGATGT CCAACACAGC 
TATCAGCAAT CGCAAATGTA TAGATCTTCT GCCGAAGATC ACTACGAACT ACAACAGGCA 
CAGTATGGGT ACAGCAATTC TAATGGGAGC ACAGTATGTG GCCCACGTAC CGGTGGTCCA 
AACTTAGTTA ATAAACAGTT AGTACTTCCC TTCGTGCCAC CCAGTTTTCC AAACAAATCA 
CAAGATGGCG TAACACATCT TATAAAGCCA TCTGAATATC TAAAGAGTAT AAGTGATAAG 
CGATCCTGCC CGTCTTCAGC AAGATCCACA GATACTGAAG ATTACATGCA TATCCAGGTG 
GCTAATCAAC ATGGAGTTAA TTGTGAACCA CCAAAGCCTC CACCGCCGCC ACCACTCCCA 
ATTCATCCGT TAATGCATCA CAATGAAAAA CAAAATAGTG TAAAAACAGG AAATATTCAG 
CATGTATCTG TAGTTAATCA GGATACCGCC ACCAGGAAGC AGCATCAACC TCTCTCCGCC 
ATCTCCATTC AAGATCTTAA TAGTGTTCAG CTTCGACGTA CTGATACTCA AAAAATGCCT 
AAGCCTTACC AAATGCCTGC ACGCAGTTTA AGTATGCAGT GTCTTACCAC GTCAGCTGAT 
TCTTACCTTA AAACGGATTT AATTGCTGAA TTAAAGATTT CAAAAGACAT TACGGGCATT 
AAGAAAATGA AAGTGGAAAA ACAGTTGGCC AGTCAATTTG ACACTGAACA TTATTCAGAA 
ATTTCCAAAC AATTTTCGAC AAACAACTAT ATGGAACAGC TTTGTATGCA GATTCCAGAA 
AAAGATCAGG CAGGTAACAT CATTCCGGAT TGGAAACGTC AGATGATGGC GAAAAAGGCA 
GCCGAACGTG CCAAAAAAGA ATTCGAGGAA CGAATGGCAA AAGAAGCAGA AAATAGACGC 
CTGTCACAAA TACCTCAATG GAAACGTGAC CTACTAGCAA GGCGGGAGGA AACCGAAAAT 
AAATTAAAAG CATCAATTTA TACACCCAAG GTGGAGGAAA ATAATCGTAT AGCTGAAACT 
TGGCATCTTA AGAATCGAGC AATTTCAATT GATAACATTA ATTTAGTTTC CCCGAGCGTT 
GATTCACAGC TACTTGCTTC GGGAACTTTT GACAGTAACA AAGAAAATAA GGATCAAAAT 
GAACAAGCCC TAATAAGTAA CATTCTAACA ACGGATGAAG ACATCAATAG TAATCAATAT 
AGTAAAGGAG ATGACGCCGA TAATATAATA CCATGGCGGG CCCAGTTACG AAAAACTAAT 
AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185143.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183445.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa199152
Clone ID Related Accession (Same CDS sequence)	XM_011185142.1 , XM_011185143.2 , XM_011185143.1
Accession Version	XM_011185143.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGTT ACTTCTCTAC AATCAGTGGT AGAAAAGGCT TTTTCGGTAG GATGACCACT 
AGTTTCACTT CCTATCGTCG TAGCAAAATA AAAAGTAAAT CAAACAACGA CATTTCTTCT 
ATTGTTGCTG AGATGAATGG TGTTAGTGGT ATAGTAGATA GCTTACCGCA ACACATATCT 
AACGATGATA TCTCAGATTT AGCTGAAATT ATGGACAAAA CTGGGCGAAA AGAGAGTAAA 
CTCTGCAATG AAGACGTAGC TGTTGTACGC AAAGAACATA ACAATCGGCG CAGCGTTGTA 
AGCGCGAACA CTCAAATGGA CAACGATGTC ACACCGGTCT ACTTAGCAGC GCAAGAAGGG 
CACTTGGAGG TGCTAAAATT TTTAGTTCTT GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA 
CGCGACGGAA TGGCACCGAT ACATGCTGCT TCGCAAATGG GATGTTTGGA TTGCCTTAGA 
TGGATGGTGC AAGATCAGGG TGTTGATCCA AATCTACGAG ATGGCGATGG GGCTACACCG 
CTGCACTTTG CCGCGTCAAG AGGACACCTT TCTGTTGTAC GGTGGTTACT CAAACATGGT 
GCGAAACTAT CATTAGACAA GTATGGCAAG TCCCCAATTA ACGATGCTGC AGAAAACCAA 
CAAGTAGAGT GTCTTAATGT GTTAGTGCAA CATGGTAGTA CAATAGATTA TGCTTGTCGT 
GACAACATCC CACTACAACG ACAGAAAGCA TGCTCCTTGT CTAATAAGGA ATTTCGAGAC 
ATACACAATT GCAATTTCAA TAGTACTGAA GGAGGTAACT GTATCAATAG CAGCTCACAC 
GGGAGCTGTA CCTCGAATAG TAGTAAACAG ACTAGTTGCA CCAACACCAT CAAATCAAAA 
ACTTCTTCGA CTTTAAGCTC AGATATTGAG CCCTTTTACT TACATCCCCC ATCGATCTCT 
ATGGCCCATA AACGCATTGA TGCCATTTAT GCACATTCTC AACAATCACG GTCATCGTCT 
GAGAAATTAT ACAATGGACA AATGGTGCCG AATGATGGCT TGTATATAAA TCCAATGCGT 
AACAGTTTCT TTTCTCCCCC TTCCCCGACT GGCAGTGTTT CTGGAGAATC GTTTTTCCTC 
CACGACCCTC ATGAGCTAAT TTATAATCGA GTTCGGGAGT TGTTCGATTC CGATTGTACC 
AGTATTGAAA ATCAAAAACC GAAAATGAAT CGCCACAAAT TTCACAGTCA TAGTAAAATG 
CATAAATCAA ATACCATAAA CAATGCAGTT GTTGTACAAG CGGATGTGCA CTCCAGTTCA 
AGTGGGACAG GCAGCGGATC AGAAGAATCT ATTTCCGCTT CTATTCAATC AAGCATGAAT 
TTATCCAAAA CAAACAATCT TCGAAGTCAA AGTTTTAATC TTCACACTAA ATGTAACAAT 
GAAATTAGTA GCCACGATCA AGGCTTCAAC GCCAGTTCGA GTACAAAGAC CATCACCAAT 
GATAATAAAA TAATTAATGC AAATTTAACC TCTGGAATCA ACTACAATAA AATCAACAAC 
AATAATATTA AAATTGATAA ACAAAAACAA ACAGCTAAGC AATCCAAAAG TAATTATTTA 
AACAATGAGA AGAAGAAACC AGAAAGTTCA CATGACCATG ATTACGAGGA TATATATTTG 
GTTCGTGAGG AAGCGCGGAA AAACAAAAAC AAATACGGTT CCGGGCGATC ACGGTCCCGT 
GATAGTGGGT CGCATTCACG TTCAGCTAGC ACTTCCTCAA CTCGAAGCTC AGACTTCGTA 
CTACAATATA GCAATCATAA TATGAACAAC AAACATCAAA GTGAAGCACT GAATCGAAAT 
AAATCGCAAT CGACTATTGG AATACATAGC AAACCCAAAT ACGAGTTATC GCAAAAGGAA 
AATTTCACCG TTAAAAATGT GAATCTAAAG AACCTGCTGA ACGTCAATAG CAATACCAAT 
GGTGGTGTTA AAAGCGACAC CTATGAAAGC GTGTGTCCGA TAGAAGATAT TGCTGAGCGC 
ACAAAGCAAT CACAGAAAAA CTCCGTTATT CGAAATAATC TTGATTGTGG TAGCATTGGT 
AACACCAGCA ATATATTCCA CCCAATATGC AATGATGTCA GCACATGCGA TCGCCAGTTG 
AAACGAGTTT CTTCTGCTCC GCCTATACAA AACATAGAAA TCGTAAGTGG ACCGCCACCA 
CCTCCACTGC CGCCACCGAT GAACGCTCCC TTACTCAAGA GAATGCAATT AGTTGCATCA 
GATCCCATGA ACTTTTGCAT TAGTCACTCA AATAGCAACT TGGATAATAT AGATTCAGAT 
TCAGGGCTTG AGGTAATTGA GGAACCTAGT TTACGTCCGT CTGAATTGAT CAGAAGCAAA 
CACAACCGAA CGATGTCTAC CATATCAGCA AACAAGAAAG CAAAACTTCT TAATGGCGGA 
TGTAATGGAA ATATACCCAA CTCTGCTCCA ATTGCCAATT GTGTTACACC AAATAGAGAA 
AACAGTAATT ATCATGATAA AAGCTTTGCT TGTGATGGTT ATCACGTTTG CAATGATCAA 
CAAGATGATA GAGTTCAGGT GCGCAATTAC CATTCACACC ATATAGATGT CCAACACAGC 
TATCAGCAAT CGCAAATGTA TAGATCTTCT GCCGAAGATC ACTACGAACT ACAACAGGCA 
CAGTATGGGT ACAGCAATTC TAATGGGAGC ACAGTATGTG GCCCACGTAC CGGTGGTCCA 
AACTTAGTTA ATAAACAGTT AGTACTTCCC TTCGTGCCAC CCAGTTTTCC AAACAAATCA 
CAAGATGGCG TAACACATCT TATAAAGCCA TCTGAATATC TAAAGAGTAT AAGTGATAAG 
CGATCCTGCC CGTCTTCAGC AAGATCCACA GATACTGAAG ATTACATGCA TATCCAGGTG 
GCTAATCAAC ATGGAGTTAA TTGTGAACCA CCAAAGCCTC CACCGCCGCC ACCACTCCCA 
ATTCATCCGT TAATGCATCA CAATGAAAAA CAAAATAGTG TAAAAACAGG AAATATTCAG 
CATGTATCTG TAGTTAATCA GGATACCGCC ACCAGGAAGC AGCATCAACC TCTCTCCGCC 
ATCTCCATTC AAGATCTTAA TAGTGTTCAG CTTCGACGTA CTGATACTCA AAAAATGCCT 
AAGCCTTACC AAATGCCTGC ACGCAGTTTA AGTATGCAGT GTCTTACCAC GTCAGCTGAT 
TCTTACCTTA AAACGGATTT AATTGCTGAA TTAAAGATTT CAAAAGACAT TACGGGCATT 
AAGAAAATGA AAGTGGAAAA ACAGTTGGCC AGTCAATTTG ACACTGAACA TTATTCAGAA 
ATTTCCAAAC AATTTTCGAC AAACAACTAT ATGGAACAGC TTTGTATGCA GATTCCAGAA 
AAAGATCAGG CAGGTAACAT CATTCCGGAT TGGAAACGTC AGATGATGGC GAAAAAGGCA 
GCCGAACGTG CCAAAAAAGA ATTCGAGGAA CGAATGGCAA AAGAAGCAGA AAATAGACGC 
CTGTCACAAA TACCTCAATG GAAACGTGAC CTACTAGCAA GGCGGGAGGA AACCGAAAAT 
AAATTAAAAG CATCAATTTA TACACCCAAG GTGGAGGAAA ATAATCGTAT AGCTGAAACT 
TGGCATCTTA AGAATCGAGC AATTTCAATT GATAACATTA ATTTAGTTTC CCCGAGCGTT 
GATTCACAGC TACTTGCTTC GGGAACTTTT GACAGTAACA AAGAAAATAA GGATCAAAAT 
GAACAAGCCC TAATAAGTAA CATTCTAACA ACGGATGAAG ACATCAATAG TAATCAATAT 
AGTAAAGGAG ATGACGCCGA TAATATAATA CCATGGCGGG CCCAGTTACG AAAAACTAAT 
AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183445.1
CDS	465..4385
Translation	MKSYFSTISGRKGFFGRMTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185143.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185143.1

ORF Insert Sequence:

ATGAAAAGTT ACTTCTCTAC AATCAGTGGT AGAAAAGGCT TTTTCGGTAG GATGACCACT 
AGTTTCACTT CCTATCGTCG TAGCAAAATA AAAAGTAAAT CAAACAACGA CATTTCTTCT 
ATTGTTGCTG AGATGAATGG TGTTAGTGGT ATAGTAGATA GCTTACCGCA ACACATATCT 
AACGATGATA TCTCAGATTT AGCTGAAATT ATGGACAAAA CTGGGCGAAA AGAGAGTAAA 
CTCTGCAATG AAGACGTAGC TGTTGTACGC AAAGAACATA ACAATCGGCG CAGCGTTGTA 
AGCGCGAACA CTCAAATGGA CAACGATGTC ACACCGGTCT ACTTAGCAGC GCAAGAAGGG 
CACTTGGAGG TGCTAAAATT TTTAGTTCTT GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA 
CGCGACGGAA TGGCACCGAT ACATGCTGCT TCGCAAATGG GATGTTTGGA TTGCCTTAGA 
TGGATGGTGC AAGATCAGGG TGTTGATCCA AATCTACGAG ATGGCGATGG GGCTACACCG 
CTGCACTTTG CCGCGTCAAG AGGACACCTT TCTGTTGTAC GGTGGTTACT CAAACATGGT 
GCGAAACTAT CATTAGACAA GTATGGCAAG TCCCCAATTA ACGATGCTGC AGAAAACCAA 
CAAGTAGAGT GTCTTAATGT GTTAGTGCAA CATGGTAGTA CAATAGATTA TGCTTGTCGT 
GACAACATCC CACTACAACG ACAGAAAGCA TGCTCCTTGT CTAATAAGGA ATTTCGAGAC 
ATACACAATT GCAATTTCAA TAGTACTGAA GGAGGTAACT GTATCAATAG CAGCTCACAC 
GGGAGCTGTA CCTCGAATAG TAGTAAACAG ACTAGTTGCA CCAACACCAT CAAATCAAAA 
ACTTCTTCGA CTTTAAGCTC AGATATTGAG CCCTTTTACT TACATCCCCC ATCGATCTCT 
ATGGCCCATA AACGCATTGA TGCCATTTAT GCACATTCTC AACAATCACG GTCATCGTCT 
GAGAAATTAT ACAATGGACA AATGGTGCCG AATGATGGCT TGTATATAAA TCCAATGCGT 
AACAGTTTCT TTTCTCCCCC TTCCCCGACT GGCAGTGTTT CTGGAGAATC GTTTTTCCTC 
CACGACCCTC ATGAGCTAAT TTATAATCGA GTTCGGGAGT TGTTCGATTC CGATTGTACC 
AGTATTGAAA ATCAAAAACC GAAAATGAAT CGCCACAAAT TTCACAGTCA TAGTAAAATG 
CATAAATCAA ATACCATAAA CAATGCAGTT GTTGTACAAG CGGATGTGCA CTCCAGTTCA 
AGTGGGACAG GCAGCGGATC AGAAGAATCT ATTTCCGCTT CTATTCAATC AAGCATGAAT 
TTATCCAAAA CAAACAATCT TCGAAGTCAA AGTTTTAATC TTCACACTAA ATGTAACAAT 
GAAATTAGTA GCCACGATCA AGGCTTCAAC GCCAGTTCGA GTACAAAGAC CATCACCAAT 
GATAATAAAA TAATTAATGC AAATTTAACC TCTGGAATCA ACTACAATAA AATCAACAAC 
AATAATATTA AAATTGATAA ACAAAAACAA ACAGCTAAGC AATCCAAAAG TAATTATTTA 
AACAATGAGA AGAAGAAACC AGAAAGTTCA CATGACCATG ATTACGAGGA TATATATTTG 
GTTCGTGAGG AAGCGCGGAA AAACAAAAAC AAATACGGTT CCGGGCGATC ACGGTCCCGT 
GATAGTGGGT CGCATTCACG TTCAGCTAGC ACTTCCTCAA CTCGAAGCTC AGACTTCGTA 
CTACAATATA GCAATCATAA TATGAACAAC AAACATCAAA GTGAAGCACT GAATCGAAAT 
AAATCGCAAT CGACTATTGG AATACATAGC AAACCCAAAT ACGAGTTATC GCAAAAGGAA 
AATTTCACCG TTAAAAATGT GAATCTAAAG AACCTGCTGA ACGTCAATAG CAATACCAAT 
GGTGGTGTTA AAAGCGACAC CTATGAAAGC GTGTGTCCGA TAGAAGATAT TGCTGAGCGC 
ACAAAGCAAT CACAGAAAAA CTCCGTTATT CGAAATAATC TTGATTGTGG TAGCATTGGT 
AACACCAGCA ATATATTCCA CCCAATATGC AATGATGTCA GCACATGCGA TCGCCAGTTG 
AAACGAGTTT CTTCTGCTCC GCCTATACAA AACATAGAAA TCGTAAGTGG ACCGCCACCA 
CCTCCACTGC CGCCACCGAT GAACGCTCCC TTACTCAAGA GAATGCAATT AGTTGCATCA 
GATCCCATGA ACTTTTGCAT TAGTCACTCA AATAGCAACT TGGATAATAT AGATTCAGAT 
TCAGGGCTTG AGGTAATTGA GGAACCTAGT TTACGTCCGT CTGAATTGAT CAGAAGCAAA 
CACAACCGAA CGATGTCTAC CATATCAGCA AACAAGAAAG CAAAACTTCT TAATGGCGGA 
TGTAATGGAA ATATACCCAA CTCTGCTCCA ATTGCCAATT GTGTTACACC AAATAGAGAA 
AACAGTAATT ATCATGATAA AAGCTTTGCT TGTGATGGTT ATCACGTTTG CAATGATCAA 
CAAGATGATA GAGTTCAGGT GCGCAATTAC CATTCACACC ATATAGATGT CCAACACAGC 
TATCAGCAAT CGCAAATGTA TAGATCTTCT GCCGAAGATC ACTACGAACT ACAACAGGCA 
CAGTATGGGT ACAGCAATTC TAATGGGAGC ACAGTATGTG GCCCACGTAC CGGTGGTCCA 
AACTTAGTTA ATAAACAGTT AGTACTTCCC TTCGTGCCAC CCAGTTTTCC AAACAAATCA 
CAAGATGGCG TAACACATCT TATAAAGCCA TCTGAATATC TAAAGAGTAT AAGTGATAAG 
CGATCCTGCC CGTCTTCAGC AAGATCCACA GATACTGAAG ATTACATGCA TATCCAGGTG 
GCTAATCAAC ATGGAGTTAA TTGTGAACCA CCAAAGCCTC CACCGCCGCC ACCACTCCCA 
ATTCATCCGT TAATGCATCA CAATGAAAAA CAAAATAGTG TAAAAACAGG AAATATTCAG 
CATGTATCTG TAGTTAATCA GGATACCGCC ACCAGGAAGC AGCATCAACC TCTCTCCGCC 
ATCTCCATTC AAGATCTTAA TAGTGTTCAG CTTCGACGTA CTGATACTCA AAAAATGCCT 
AAGCCTTACC AAATGCCTGC ACGCAGTTTA AGTATGCAGT GTCTTACCAC GTCAGCTGAT 
TCTTACCTTA AAACGGATTT AATTGCTGAA TTAAAGATTT CAAAAGACAT TACGGGCATT 
AAGAAAATGA AAGTGGAAAA ACAGTTGGCC AGTCAATTTG ACACTGAACA TTATTCAGAA 
ATTTCCAAAC AATTTTCGAC AAACAACTAT ATGGAACAGC TTTGTATGCA GATTCCAGAA 
AAAGATCAGG CAGGTAACAT CATTCCGGAT TGGAAACGTC AGATGATGGC GAAAAAGGCA 
GCCGAACGTG CCAAAAAAGA ATTCGAGGAA CGAATGGCAA AAGAAGCAGA AAATAGACGC 
CTGTCACAAA TACCTCAATG GAAACGTGAC CTACTAGCAA GGCGGGAGGA AACCGAAAAT 
AAATTAAAAG CATCAATTTA TACACCCAAG GTGGAGGAAA ATAATCGTAT AGCTGAAACT 
TGGCATCTTA AGAATCGAGC AATTTCAATT GATAACATTA ATTTAGTTTC CCCGAGCGTT 
GATTCACAGC TACTTGCTTC GGGAACTTTT GACAGTAACA AAGAAAATAA GGATCAAAAT 
GAACAAGCCC TAATAAGTAA CATTCTAACA ACGGATGAAG ACATCAATAG TAATCAATAT 
AGTAAAGGAG ATGACGCCGA TAATATAATA CCATGGCGGG CCCAGTTACG AAAAACTAAT 
AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185144.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183446.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X6, mRNA.

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CloneID	OBa199153
Clone ID Related Accession (Same CDS sequence)	XM_011185144.1 , XM_011185144.2
Accession Version	XM_011185144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTA GTTTCACTTC CTATCGTCGT AGCAAAATAA AAAGTAAATC AAACAACGAC 
ATTTCTTCTA TTGTTGCTGA GATGAATGGT GTTAGTGGTA TAGTAGATAG CTTACCGCAA 
CACATATCTA ACGATGATAT CTCAGATTTA GCTGAAATTA TGGACAAAAC TGGGCGAAAA 
GAGAGTAAAC TCTGCAATGA AGACGTAGCT GTTGTACGCA AAGAACATAA CAATCGGCGC 
AGCGTTGTAA GCGCGAACAC TCAAATGGAC AACGATGTCA CACCGGTCTA CTTAGCAGCG 
CAAGAAGGGC ACTTGGAGGT GCTAAAATTT TTAGTTCTTG AAGCTGGCGG TTCTCTCTAT 
GTGCGAGCAC GCGACGGAAT GGCACCGATA CATGCTGCTT CGCAAATGGG ATGTTTGGAT 
TGCCTTAGAT GGATGGTGCA AGATCAGGGT GTTGATCCAA ATCTACGAGA TGGCGATGGG 
GCTACACCGC TGCACTTTGC CGCGTCAAGA GGACACCTTT CTGTTGTACG GTGGTTACTC 
AAACATGGTG CGAAACTATC ATTAGACAAG TATGGCAAGT CCCCAATTAA CGATGCTGCA 
GAAAACCAAC AAGTAGAGTG TCTTAATGTG TTAGTGCAAC ATGGTAGTAC AATAGATTAT 
GCTTGTCGTG ACAACATCCC ACTACAACGA CAGAAAGCAT GCTCCTTGTC TAATAAGGAA 
TTTCGAGACA TACACAATTG CAATTTCAAT AGTACTGAAG GAGGTAACTG TATCAATAGC 
AGCTCACACG GGAGCTGTAC CTCGAATAGT AGTAAACAGA CTAGTTGCAC CAACACCATC 
AAATCAAAAA CTTCTTCGAC TTTAAGCTCA GATATTGAGC CCTTTTACTT ACATCCCCCA 
TCGATCTCTA TGGCCCATAA ACGCATTGAT GCCATTTATG CACATTCTCA ACAATCACGG 
TCATCGTCTG AGAAATTATA CAATGGACAA ATGGTGCCGA ATGATGGCTT GTATATAAAT 
CCAATGCGTA ACAGTTTCTT TTCTCCCCCT TCCCCGACTG GCAGTGTTTC TGGAGAATCG 
TTTTTCCTCC ACGACCCTCA TGAGCTAATT TATAATCGAG TTCGGGAGTT GTTCGATTCC 
GATTGTACCA GTATTGAAAA TCAAAAACCG AAAATGAATC GCCACAAATT TCACAGTCAT 
AGTAAAATGC ATAAATCAAA TACCATAAAC AATGCAGTTG TTGTACAAGC GGATGTGCAC 
TCCAGTTCAA GTGGGACAGG CAGCGGATCA GAAGAATCTA TTTCCGCTTC TATTCAATCA 
AGCATGAATT TATCCAAAAC AAACAATCTT CGAAGTCAAA GTTTTAATCT TCACACTAAA 
TGTAACAATG AAATTAGTAG CCACGATCAA GGCTTCAACG CCAGTTCGAG TACAAAGACC 
ATCACCAATG ATAATAAAAT AATTAATGCA AATTTAACCT CTGGAATCAA CTACAATAAA 
ATCAACAACA ATAATATTAA AATTGATAAA CAAAAACAAA CAGCTAAGCA ATCCAAAAGT 
AATTATTTAA ACAATGAGAA GAAGAAACCA GAAAGTTCAC ATGACCATGA TTACGAGGAT 
ATATATTTGG TTCGTGAGGA AGCGCGGAAA AACAAAAACA AATACGGTTC CGGGCGATCA 
CGGTCCCGTG ATAGTGGGTC GCATTCACGT TCAGCTAGCA CTTCCTCAAC TCGAAGCTCA 
GACTTCGTAC TACAATATAG CAATCATAAT ATGAACAACA AACATCAAAG TGAAGCACTG 
AATCGAAATA AATCGCAATC GACTATTGGA ATACATAGCA AACCCAAATA CGAGTTATCG 
CAAAAGGAAA ATTTCACCGT TAAAAATGTG AATCTAAAGA ACCTGCTGAA CGTCAATAGC 
AATACCAATG GTGGTGTTAA AAGCGACACC TATGAAAGCG TGTGTCCGAT AGAAGATATT 
GCTGAGCGCA CAAAGCAATC ACAGAAAAAC TCCGTTATTC GAAATAATCT TGATTGTGGT 
AGCATTGGTA ACACCAGCAA TATATTCCAC CCAATATGCA ATGATGTCAG CACATGCGAT 
CGCCAGTTGA AACGAGTTTC TTCTGCTCCG CCTATACAAA ACATAGAAAT CGTAAGTGGA 
CCGCCACCAC CTCCACTGCC GCCACCGATG AACGCTCCCT TACTCAAGAG AATGCAATTA 
GTTGCATCAG ATCCCATGAA CTTTTGCATT AGTCACTCAA ATAGCAACTT GGATAATATA 
GATTCAGATT CAGGGCTTGA GGTAATTGAG GAACCTAGTT TACGTCCGTC TGAATTGATC 
AGAAGCAAAC ACAACCGAAC GATGTCTACC ATATCAGCAA ACAAGAAAGC AAAACTTCTT 
AATGGCGGAT GTAATGGAAA TATACCCAAC TCTGCTCCAA TTGCCAATTG TGTTACACCA 
AATAGAGAAA ACAGTAATTA TCATGATAAA AGCTTTGCTT GTGATGGTTA TCACGTTTGC 
AATGATCAAC AAGATGATAG AGTTCAGGTG CGCAATTACC ATTCACACCA TATAGATGTC 
CAACACAGCT ATCAGCAATC GCAAATGTAT AGATCTTCTG CCGAAGATCA CTACGAACTA 
CAACAGGCAC AGTATGGGTA CAGCAATTCT AATGGGAGCA CAGTATGTGG CCCACGTACC 
GGTGGTCCAA ACTTAGTTAA TAAACAGTTA GTACTTCCCT TCGTGCCACC CAGTTTTCCA 
AACAAATCAC AAGATGGCGT AACACATCTT ATAAAGCCAT CTGAATATCT AAAGAGTATA 
AGTGATAAGC GATCCTGCCC GTCTTCAGCA AGATCCACAG ATACTGAAGA TTACATGCAT 
ATCCAGGTGG CTAATCAACA TGGAGTTAAT TGTGAACCAC CAAAGCCTCC ACCGCCGCCA 
CCACTCCCAA TTCATCCGTT AATGCATCAC AATGAAAAAC AAAATAGTGT AAAAACAGGA 
AATATTCAGC ATGTATCTGT AGTTAATCAG GATACCGCCA CCAGGAAGCA GCATCAACCT 
CTCTCCGCCA TCTCCATTCA AGATCTTAAT AGTGTTCAGC TTCGACGTAC TGATACTCAA 
AAAATGCCTA AGCCTTACCA AATGCCTGCA CGCAGTTTAA GTATGCAGTG TCTTACCACG 
TCAGCTGATT CTTACCTTAA AACGGATTTA ATTGCTGAAT TAAAGATTTC AAAAGACATT 
ACGGGCATTA AGAAAATGAA AGTGGAAAAA CAGTTGGCCA GTCAATTTGA CACTGAACAT 
TATTCAGAAA TTTCCAAACA ATTTTCGACA AACAACTATA TGGAACAGCT TTGTATGCAG 
ATTCCAGAAA AAGATCAGGC AGGTAACATC ATTCCGGATT GGAAACGTCA GATGATGGCG 
AAAAAGGCAG CCGAACGTGC CAAAAAAGAA TTCGAGGAAC GAATGGCAAA AGAAGCAGAA 
AATAGACGCC TGTCACAAAT ACCTCAATGG AAACGTGACC TACTAGCAAG GCGGGAGGAA 
ACCGAAAATA AATTAAAAGC ATCAATTTAT ACACCCAAGG TGGAGGAAAA TAATCGTATA 
GCTGAAACTT GGCATCTTAA GAATCGAGCA ATTTCAATTG ATAACATTAA TTTAGTTTCC 
CCGAGCGTTG ATTCACAGCT ACTTGCTTCG GGAACTTTTG ACAGTAACAA AGAAAATAAG 
GATCAAAATG AACAAGCCCT AATAAGTAAC ATTCTAACAA CGGATGAAGA CATCAATAGT 
AATCAATATA GTAAAGGAGA TGACGCCGAT AATATAATAC CATGGCGGGC CCAGTTACGA 
AAAACTAATA GTAGACTAAG TTTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183446.1
CDS	886..4755
Translation	MTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185144.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185144.1

ORF Insert Sequence:

ATGACCACTA GTTTCACTTC CTATCGTCGT AGCAAAATAA AAAGTAAATC AAACAACGAC 
ATTTCTTCTA TTGTTGCTGA GATGAATGGT GTTAGTGGTA TAGTAGATAG CTTACCGCAA 
CACATATCTA ACGATGATAT CTCAGATTTA GCTGAAATTA TGGACAAAAC TGGGCGAAAA 
GAGAGTAAAC TCTGCAATGA AGACGTAGCT GTTGTACGCA AAGAACATAA CAATCGGCGC 
AGCGTTGTAA GCGCGAACAC TCAAATGGAC AACGATGTCA CACCGGTCTA CTTAGCAGCG 
CAAGAAGGGC ACTTGGAGGT GCTAAAATTT TTAGTTCTTG AAGCTGGCGG TTCTCTCTAT 
GTGCGAGCAC GCGACGGAAT GGCACCGATA CATGCTGCTT CGCAAATGGG ATGTTTGGAT 
TGCCTTAGAT GGATGGTGCA AGATCAGGGT GTTGATCCAA ATCTACGAGA TGGCGATGGG 
GCTACACCGC TGCACTTTGC CGCGTCAAGA GGACACCTTT CTGTTGTACG GTGGTTACTC 
AAACATGGTG CGAAACTATC ATTAGACAAG TATGGCAAGT CCCCAATTAA CGATGCTGCA 
GAAAACCAAC AAGTAGAGTG TCTTAATGTG TTAGTGCAAC ATGGTAGTAC AATAGATTAT 
GCTTGTCGTG ACAACATCCC ACTACAACGA CAGAAAGCAT GCTCCTTGTC TAATAAGGAA 
TTTCGAGACA TACACAATTG CAATTTCAAT AGTACTGAAG GAGGTAACTG TATCAATAGC 
AGCTCACACG GGAGCTGTAC CTCGAATAGT AGTAAACAGA CTAGTTGCAC CAACACCATC 
AAATCAAAAA CTTCTTCGAC TTTAAGCTCA GATATTGAGC CCTTTTACTT ACATCCCCCA 
TCGATCTCTA TGGCCCATAA ACGCATTGAT GCCATTTATG CACATTCTCA ACAATCACGG 
TCATCGTCTG AGAAATTATA CAATGGACAA ATGGTGCCGA ATGATGGCTT GTATATAAAT 
CCAATGCGTA ACAGTTTCTT TTCTCCCCCT TCCCCGACTG GCAGTGTTTC TGGAGAATCG 
TTTTTCCTCC ACGACCCTCA TGAGCTAATT TATAATCGAG TTCGGGAGTT GTTCGATTCC 
GATTGTACCA GTATTGAAAA TCAAAAACCG AAAATGAATC GCCACAAATT TCACAGTCAT 
AGTAAAATGC ATAAATCAAA TACCATAAAC AATGCAGTTG TTGTACAAGC GGATGTGCAC 
TCCAGTTCAA GTGGGACAGG CAGCGGATCA GAAGAATCTA TTTCCGCTTC TATTCAATCA 
AGCATGAATT TATCCAAAAC AAACAATCTT CGAAGTCAAA GTTTTAATCT TCACACTAAA 
TGTAACAATG AAATTAGTAG CCACGATCAA GGCTTCAACG CCAGTTCGAG TACAAAGACC 
ATCACCAATG ATAATAAAAT AATTAATGCA AATTTAACCT CTGGAATCAA CTACAATAAA 
ATCAACAACA ATAATATTAA AATTGATAAA CAAAAACAAA CAGCTAAGCA ATCCAAAAGT 
AATTATTTAA ACAATGAGAA GAAGAAACCA GAAAGTTCAC ATGACCATGA TTACGAGGAT 
ATATATTTGG TTCGTGAGGA AGCGCGGAAA AACAAAAACA AATACGGTTC CGGGCGATCA 
CGGTCCCGTG ATAGTGGGTC GCATTCACGT TCAGCTAGCA CTTCCTCAAC TCGAAGCTCA 
GACTTCGTAC TACAATATAG CAATCATAAT ATGAACAACA AACATCAAAG TGAAGCACTG 
AATCGAAATA AATCGCAATC GACTATTGGA ATACATAGCA AACCCAAATA CGAGTTATCG 
CAAAAGGAAA ATTTCACCGT TAAAAATGTG AATCTAAAGA ACCTGCTGAA CGTCAATAGC 
AATACCAATG GTGGTGTTAA AAGCGACACC TATGAAAGCG TGTGTCCGAT AGAAGATATT 
GCTGAGCGCA CAAAGCAATC ACAGAAAAAC TCCGTTATTC GAAATAATCT TGATTGTGGT 
AGCATTGGTA ACACCAGCAA TATATTCCAC CCAATATGCA ATGATGTCAG CACATGCGAT 
CGCCAGTTGA AACGAGTTTC TTCTGCTCCG CCTATACAAA ACATAGAAAT CGTAAGTGGA 
CCGCCACCAC CTCCACTGCC GCCACCGATG AACGCTCCCT TACTCAAGAG AATGCAATTA 
GTTGCATCAG ATCCCATGAA CTTTTGCATT AGTCACTCAA ATAGCAACTT GGATAATATA 
GATTCAGATT CAGGGCTTGA GGTAATTGAG GAACCTAGTT TACGTCCGTC TGAATTGATC 
AGAAGCAAAC ACAACCGAAC GATGTCTACC ATATCAGCAA ACAAGAAAGC AAAACTTCTT 
AATGGCGGAT GTAATGGAAA TATACCCAAC TCTGCTCCAA TTGCCAATTG TGTTACACCA 
AATAGAGAAA ACAGTAATTA TCATGATAAA AGCTTTGCTT GTGATGGTTA TCACGTTTGC 
AATGATCAAC AAGATGATAG AGTTCAGGTG CGCAATTACC ATTCACACCA TATAGATGTC 
CAACACAGCT ATCAGCAATC GCAAATGTAT AGATCTTCTG CCGAAGATCA CTACGAACTA 
CAACAGGCAC AGTATGGGTA CAGCAATTCT AATGGGAGCA CAGTATGTGG CCCACGTACC 
GGTGGTCCAA ACTTAGTTAA TAAACAGTTA GTACTTCCCT TCGTGCCACC CAGTTTTCCA 
AACAAATCAC AAGATGGCGT AACACATCTT ATAAAGCCAT CTGAATATCT AAAGAGTATA 
AGTGATAAGC GATCCTGCCC GTCTTCAGCA AGATCCACAG ATACTGAAGA TTACATGCAT 
ATCCAGGTGG CTAATCAACA TGGAGTTAAT TGTGAACCAC CAAAGCCTCC ACCGCCGCCA 
CCACTCCCAA TTCATCCGTT AATGCATCAC AATGAAAAAC AAAATAGTGT AAAAACAGGA 
AATATTCAGC ATGTATCTGT AGTTAATCAG GATACCGCCA CCAGGAAGCA GCATCAACCT 
CTCTCCGCCA TCTCCATTCA AGATCTTAAT AGTGTTCAGC TTCGACGTAC TGATACTCAA 
AAAATGCCTA AGCCTTACCA AATGCCTGCA CGCAGTTTAA GTATGCAGTG TCTTACCACG 
TCAGCTGATT CTTACCTTAA AACGGATTTA ATTGCTGAAT TAAAGATTTC AAAAGACATT 
ACGGGCATTA AGAAAATGAA AGTGGAAAAA CAGTTGGCCA GTCAATTTGA CACTGAACAT 
TATTCAGAAA TTTCCAAACA ATTTTCGACA AACAACTATA TGGAACAGCT TTGTATGCAG 
ATTCCAGAAA AAGATCAGGC AGGTAACATC ATTCCGGATT GGAAACGTCA GATGATGGCG 
AAAAAGGCAG CCGAACGTGC CAAAAAAGAA TTCGAGGAAC GAATGGCAAA AGAAGCAGAA 
AATAGACGCC TGTCACAAAT ACCTCAATGG AAACGTGACC TACTAGCAAG GCGGGAGGAA 
ACCGAAAATA AATTAAAAGC ATCAATTTAT ACACCCAAGG TGGAGGAAAA TAATCGTATA 
GCTGAAACTT GGCATCTTAA GAATCGAGCA ATTTCAATTG ATAACATTAA TTTAGTTTCC 
CCGAGCGTTG ATTCACAGCT ACTTGCTTCG GGAACTTTTG ACAGTAACAA AGAAAATAAG 
GATCAAAATG AACAAGCCCT AATAAGTAAC ATTCTAACAA CGGATGAAGA CATCAATAGT 
AATCAATATA GTAAAGGAGA TGACGCCGAT AATATAATAC CATGGCGGGC CCAGTTACGA 
AAAACTAATA GTAGACTAAG TTTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185144.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183446.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X5, mRNA.

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CloneID	OBa199153
Clone ID Related Accession (Same CDS sequence)	XM_011185144.1 , XM_011185144.2
Accession Version	XM_011185144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTA GTTTCACTTC CTATCGTCGT AGCAAAATAA AAAGTAAATC AAACAACGAC 
ATTTCTTCTA TTGTTGCTGA GATGAATGGT GTTAGTGGTA TAGTAGATAG CTTACCGCAA 
CACATATCTA ACGATGATAT CTCAGATTTA GCTGAAATTA TGGACAAAAC TGGGCGAAAA 
GAGAGTAAAC TCTGCAATGA AGACGTAGCT GTTGTACGCA AAGAACATAA CAATCGGCGC 
AGCGTTGTAA GCGCGAACAC TCAAATGGAC AACGATGTCA CACCGGTCTA CTTAGCAGCG 
CAAGAAGGGC ACTTGGAGGT GCTAAAATTT TTAGTTCTTG AAGCTGGCGG TTCTCTCTAT 
GTGCGAGCAC GCGACGGAAT GGCACCGATA CATGCTGCTT CGCAAATGGG ATGTTTGGAT 
TGCCTTAGAT GGATGGTGCA AGATCAGGGT GTTGATCCAA ATCTACGAGA TGGCGATGGG 
GCTACACCGC TGCACTTTGC CGCGTCAAGA GGACACCTTT CTGTTGTACG GTGGTTACTC 
AAACATGGTG CGAAACTATC ATTAGACAAG TATGGCAAGT CCCCAATTAA CGATGCTGCA 
GAAAACCAAC AAGTAGAGTG TCTTAATGTG TTAGTGCAAC ATGGTAGTAC AATAGATTAT 
GCTTGTCGTG ACAACATCCC ACTACAACGA CAGAAAGCAT GCTCCTTGTC TAATAAGGAA 
TTTCGAGACA TACACAATTG CAATTTCAAT AGTACTGAAG GAGGTAACTG TATCAATAGC 
AGCTCACACG GGAGCTGTAC CTCGAATAGT AGTAAACAGA CTAGTTGCAC CAACACCATC 
AAATCAAAAA CTTCTTCGAC TTTAAGCTCA GATATTGAGC CCTTTTACTT ACATCCCCCA 
TCGATCTCTA TGGCCCATAA ACGCATTGAT GCCATTTATG CACATTCTCA ACAATCACGG 
TCATCGTCTG AGAAATTATA CAATGGACAA ATGGTGCCGA ATGATGGCTT GTATATAAAT 
CCAATGCGTA ACAGTTTCTT TTCTCCCCCT TCCCCGACTG GCAGTGTTTC TGGAGAATCG 
TTTTTCCTCC ACGACCCTCA TGAGCTAATT TATAATCGAG TTCGGGAGTT GTTCGATTCC 
GATTGTACCA GTATTGAAAA TCAAAAACCG AAAATGAATC GCCACAAATT TCACAGTCAT 
AGTAAAATGC ATAAATCAAA TACCATAAAC AATGCAGTTG TTGTACAAGC GGATGTGCAC 
TCCAGTTCAA GTGGGACAGG CAGCGGATCA GAAGAATCTA TTTCCGCTTC TATTCAATCA 
AGCATGAATT TATCCAAAAC AAACAATCTT CGAAGTCAAA GTTTTAATCT TCACACTAAA 
TGTAACAATG AAATTAGTAG CCACGATCAA GGCTTCAACG CCAGTTCGAG TACAAAGACC 
ATCACCAATG ATAATAAAAT AATTAATGCA AATTTAACCT CTGGAATCAA CTACAATAAA 
ATCAACAACA ATAATATTAA AATTGATAAA CAAAAACAAA CAGCTAAGCA ATCCAAAAGT 
AATTATTTAA ACAATGAGAA GAAGAAACCA GAAAGTTCAC ATGACCATGA TTACGAGGAT 
ATATATTTGG TTCGTGAGGA AGCGCGGAAA AACAAAAACA AATACGGTTC CGGGCGATCA 
CGGTCCCGTG ATAGTGGGTC GCATTCACGT TCAGCTAGCA CTTCCTCAAC TCGAAGCTCA 
GACTTCGTAC TACAATATAG CAATCATAAT ATGAACAACA AACATCAAAG TGAAGCACTG 
AATCGAAATA AATCGCAATC GACTATTGGA ATACATAGCA AACCCAAATA CGAGTTATCG 
CAAAAGGAAA ATTTCACCGT TAAAAATGTG AATCTAAAGA ACCTGCTGAA CGTCAATAGC 
AATACCAATG GTGGTGTTAA AAGCGACACC TATGAAAGCG TGTGTCCGAT AGAAGATATT 
GCTGAGCGCA CAAAGCAATC ACAGAAAAAC TCCGTTATTC GAAATAATCT TGATTGTGGT 
AGCATTGGTA ACACCAGCAA TATATTCCAC CCAATATGCA ATGATGTCAG CACATGCGAT 
CGCCAGTTGA AACGAGTTTC TTCTGCTCCG CCTATACAAA ACATAGAAAT CGTAAGTGGA 
CCGCCACCAC CTCCACTGCC GCCACCGATG AACGCTCCCT TACTCAAGAG AATGCAATTA 
GTTGCATCAG ATCCCATGAA CTTTTGCATT AGTCACTCAA ATAGCAACTT GGATAATATA 
GATTCAGATT CAGGGCTTGA GGTAATTGAG GAACCTAGTT TACGTCCGTC TGAATTGATC 
AGAAGCAAAC ACAACCGAAC GATGTCTACC ATATCAGCAA ACAAGAAAGC AAAACTTCTT 
AATGGCGGAT GTAATGGAAA TATACCCAAC TCTGCTCCAA TTGCCAATTG TGTTACACCA 
AATAGAGAAA ACAGTAATTA TCATGATAAA AGCTTTGCTT GTGATGGTTA TCACGTTTGC 
AATGATCAAC AAGATGATAG AGTTCAGGTG CGCAATTACC ATTCACACCA TATAGATGTC 
CAACACAGCT ATCAGCAATC GCAAATGTAT AGATCTTCTG CCGAAGATCA CTACGAACTA 
CAACAGGCAC AGTATGGGTA CAGCAATTCT AATGGGAGCA CAGTATGTGG CCCACGTACC 
GGTGGTCCAA ACTTAGTTAA TAAACAGTTA GTACTTCCCT TCGTGCCACC CAGTTTTCCA 
AACAAATCAC AAGATGGCGT AACACATCTT ATAAAGCCAT CTGAATATCT AAAGAGTATA 
AGTGATAAGC GATCCTGCCC GTCTTCAGCA AGATCCACAG ATACTGAAGA TTACATGCAT 
ATCCAGGTGG CTAATCAACA TGGAGTTAAT TGTGAACCAC CAAAGCCTCC ACCGCCGCCA 
CCACTCCCAA TTCATCCGTT AATGCATCAC AATGAAAAAC AAAATAGTGT AAAAACAGGA 
AATATTCAGC ATGTATCTGT AGTTAATCAG GATACCGCCA CCAGGAAGCA GCATCAACCT 
CTCTCCGCCA TCTCCATTCA AGATCTTAAT AGTGTTCAGC TTCGACGTAC TGATACTCAA 
AAAATGCCTA AGCCTTACCA AATGCCTGCA CGCAGTTTAA GTATGCAGTG TCTTACCACG 
TCAGCTGATT CTTACCTTAA AACGGATTTA ATTGCTGAAT TAAAGATTTC AAAAGACATT 
ACGGGCATTA AGAAAATGAA AGTGGAAAAA CAGTTGGCCA GTCAATTTGA CACTGAACAT 
TATTCAGAAA TTTCCAAACA ATTTTCGACA AACAACTATA TGGAACAGCT TTGTATGCAG 
ATTCCAGAAA AAGATCAGGC AGGTAACATC ATTCCGGATT GGAAACGTCA GATGATGGCG 
AAAAAGGCAG CCGAACGTGC CAAAAAAGAA TTCGAGGAAC GAATGGCAAA AGAAGCAGAA 
AATAGACGCC TGTCACAAAT ACCTCAATGG AAACGTGACC TACTAGCAAG GCGGGAGGAA 
ACCGAAAATA AATTAAAAGC ATCAATTTAT ACACCCAAGG TGGAGGAAAA TAATCGTATA 
GCTGAAACTT GGCATCTTAA GAATCGAGCA ATTTCAATTG ATAACATTAA TTTAGTTTCC 
CCGAGCGTTG ATTCACAGCT ACTTGCTTCG GGAACTTTTG ACAGTAACAA AGAAAATAAG 
GATCAAAATG AACAAGCCCT AATAAGTAAC ATTCTAACAA CGGATGAAGA CATCAATAGT 
AATCAATATA GTAAAGGAGA TGACGCCGAT AATATAATAC CATGGCGGGC CCAGTTACGA 
AAAACTAATA GTAGACTAAG TTTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183446.1
CDS	434..4303
Translation	MTTSFTSYRRSKIKSKSNNDISSIVAEMNGVSGIVDSLPQHISNDDISDLAEIMDKTGRKESKLCNEDVAVVRKEHNNRRSVVSANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185144.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185144.2

ORF Insert Sequence:

ATGACCACTA GTTTCACTTC CTATCGTCGT AGCAAAATAA AAAGTAAATC AAACAACGAC 
ATTTCTTCTA TTGTTGCTGA GATGAATGGT GTTAGTGGTA TAGTAGATAG CTTACCGCAA 
CACATATCTA ACGATGATAT CTCAGATTTA GCTGAAATTA TGGACAAAAC TGGGCGAAAA 
GAGAGTAAAC TCTGCAATGA AGACGTAGCT GTTGTACGCA AAGAACATAA CAATCGGCGC 
AGCGTTGTAA GCGCGAACAC TCAAATGGAC AACGATGTCA CACCGGTCTA CTTAGCAGCG 
CAAGAAGGGC ACTTGGAGGT GCTAAAATTT TTAGTTCTTG AAGCTGGCGG TTCTCTCTAT 
GTGCGAGCAC GCGACGGAAT GGCACCGATA CATGCTGCTT CGCAAATGGG ATGTTTGGAT 
TGCCTTAGAT GGATGGTGCA AGATCAGGGT GTTGATCCAA ATCTACGAGA TGGCGATGGG 
GCTACACCGC TGCACTTTGC CGCGTCAAGA GGACACCTTT CTGTTGTACG GTGGTTACTC 
AAACATGGTG CGAAACTATC ATTAGACAAG TATGGCAAGT CCCCAATTAA CGATGCTGCA 
GAAAACCAAC AAGTAGAGTG TCTTAATGTG TTAGTGCAAC ATGGTAGTAC AATAGATTAT 
GCTTGTCGTG ACAACATCCC ACTACAACGA CAGAAAGCAT GCTCCTTGTC TAATAAGGAA 
TTTCGAGACA TACACAATTG CAATTTCAAT AGTACTGAAG GAGGTAACTG TATCAATAGC 
AGCTCACACG GGAGCTGTAC CTCGAATAGT AGTAAACAGA CTAGTTGCAC CAACACCATC 
AAATCAAAAA CTTCTTCGAC TTTAAGCTCA GATATTGAGC CCTTTTACTT ACATCCCCCA 
TCGATCTCTA TGGCCCATAA ACGCATTGAT GCCATTTATG CACATTCTCA ACAATCACGG 
TCATCGTCTG AGAAATTATA CAATGGACAA ATGGTGCCGA ATGATGGCTT GTATATAAAT 
CCAATGCGTA ACAGTTTCTT TTCTCCCCCT TCCCCGACTG GCAGTGTTTC TGGAGAATCG 
TTTTTCCTCC ACGACCCTCA TGAGCTAATT TATAATCGAG TTCGGGAGTT GTTCGATTCC 
GATTGTACCA GTATTGAAAA TCAAAAACCG AAAATGAATC GCCACAAATT TCACAGTCAT 
AGTAAAATGC ATAAATCAAA TACCATAAAC AATGCAGTTG TTGTACAAGC GGATGTGCAC 
TCCAGTTCAA GTGGGACAGG CAGCGGATCA GAAGAATCTA TTTCCGCTTC TATTCAATCA 
AGCATGAATT TATCCAAAAC AAACAATCTT CGAAGTCAAA GTTTTAATCT TCACACTAAA 
TGTAACAATG AAATTAGTAG CCACGATCAA GGCTTCAACG CCAGTTCGAG TACAAAGACC 
ATCACCAATG ATAATAAAAT AATTAATGCA AATTTAACCT CTGGAATCAA CTACAATAAA 
ATCAACAACA ATAATATTAA AATTGATAAA CAAAAACAAA CAGCTAAGCA ATCCAAAAGT 
AATTATTTAA ACAATGAGAA GAAGAAACCA GAAAGTTCAC ATGACCATGA TTACGAGGAT 
ATATATTTGG TTCGTGAGGA AGCGCGGAAA AACAAAAACA AATACGGTTC CGGGCGATCA 
CGGTCCCGTG ATAGTGGGTC GCATTCACGT TCAGCTAGCA CTTCCTCAAC TCGAAGCTCA 
GACTTCGTAC TACAATATAG CAATCATAAT ATGAACAACA AACATCAAAG TGAAGCACTG 
AATCGAAATA AATCGCAATC GACTATTGGA ATACATAGCA AACCCAAATA CGAGTTATCG 
CAAAAGGAAA ATTTCACCGT TAAAAATGTG AATCTAAAGA ACCTGCTGAA CGTCAATAGC 
AATACCAATG GTGGTGTTAA AAGCGACACC TATGAAAGCG TGTGTCCGAT AGAAGATATT 
GCTGAGCGCA CAAAGCAATC ACAGAAAAAC TCCGTTATTC GAAATAATCT TGATTGTGGT 
AGCATTGGTA ACACCAGCAA TATATTCCAC CCAATATGCA ATGATGTCAG CACATGCGAT 
CGCCAGTTGA AACGAGTTTC TTCTGCTCCG CCTATACAAA ACATAGAAAT CGTAAGTGGA 
CCGCCACCAC CTCCACTGCC GCCACCGATG AACGCTCCCT TACTCAAGAG AATGCAATTA 
GTTGCATCAG ATCCCATGAA CTTTTGCATT AGTCACTCAA ATAGCAACTT GGATAATATA 
GATTCAGATT CAGGGCTTGA GGTAATTGAG GAACCTAGTT TACGTCCGTC TGAATTGATC 
AGAAGCAAAC ACAACCGAAC GATGTCTACC ATATCAGCAA ACAAGAAAGC AAAACTTCTT 
AATGGCGGAT GTAATGGAAA TATACCCAAC TCTGCTCCAA TTGCCAATTG TGTTACACCA 
AATAGAGAAA ACAGTAATTA TCATGATAAA AGCTTTGCTT GTGATGGTTA TCACGTTTGC 
AATGATCAAC AAGATGATAG AGTTCAGGTG CGCAATTACC ATTCACACCA TATAGATGTC 
CAACACAGCT ATCAGCAATC GCAAATGTAT AGATCTTCTG CCGAAGATCA CTACGAACTA 
CAACAGGCAC AGTATGGGTA CAGCAATTCT AATGGGAGCA CAGTATGTGG CCCACGTACC 
GGTGGTCCAA ACTTAGTTAA TAAACAGTTA GTACTTCCCT TCGTGCCACC CAGTTTTCCA 
AACAAATCAC AAGATGGCGT AACACATCTT ATAAAGCCAT CTGAATATCT AAAGAGTATA 
AGTGATAAGC GATCCTGCCC GTCTTCAGCA AGATCCACAG ATACTGAAGA TTACATGCAT 
ATCCAGGTGG CTAATCAACA TGGAGTTAAT TGTGAACCAC CAAAGCCTCC ACCGCCGCCA 
CCACTCCCAA TTCATCCGTT AATGCATCAC AATGAAAAAC AAAATAGTGT AAAAACAGGA 
AATATTCAGC ATGTATCTGT AGTTAATCAG GATACCGCCA CCAGGAAGCA GCATCAACCT 
CTCTCCGCCA TCTCCATTCA AGATCTTAAT AGTGTTCAGC TTCGACGTAC TGATACTCAA 
AAAATGCCTA AGCCTTACCA AATGCCTGCA CGCAGTTTAA GTATGCAGTG TCTTACCACG 
TCAGCTGATT CTTACCTTAA AACGGATTTA ATTGCTGAAT TAAAGATTTC AAAAGACATT 
ACGGGCATTA AGAAAATGAA AGTGGAAAAA CAGTTGGCCA GTCAATTTGA CACTGAACAT 
TATTCAGAAA TTTCCAAACA ATTTTCGACA AACAACTATA TGGAACAGCT TTGTATGCAG 
ATTCCAGAAA AAGATCAGGC AGGTAACATC ATTCCGGATT GGAAACGTCA GATGATGGCG 
AAAAAGGCAG CCGAACGTGC CAAAAAAGAA TTCGAGGAAC GAATGGCAAA AGAAGCAGAA 
AATAGACGCC TGTCACAAAT ACCTCAATGG AAACGTGACC TACTAGCAAG GCGGGAGGAA 
ACCGAAAATA AATTAAAAGC ATCAATTTAT ACACCCAAGG TGGAGGAAAA TAATCGTATA 
GCTGAAACTT GGCATCTTAA GAATCGAGCA ATTTCAATTG ATAACATTAA TTTAGTTTCC 
CCGAGCGTTG ATTCACAGCT ACTTGCTTCG GGAACTTTTG ACAGTAACAA AGAAAATAAG 
GATCAAAATG AACAAGCCCT AATAAGTAAC ATTCTAACAA CGGATGAAGA CATCAATAGT 
AATCAATATA GTAAAGGAGA TGACGCCGAT AATATAATAC CATGGCGGGC CCAGTTACGA 
AAAACTAATA GTAGACTAAG TTTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185145.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183447.1 Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa199154
Clone ID Related Accession (Same CDS sequence)	XM_011185145.1 , XM_011185145.2
Accession Version	XM_011185145.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105212834 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGCGCGAACA CTCAAATGGA CAACGATGTC 
ACACCGGTCT ACTTAGCAGC GCAAGAAGGG CACTTGGAGG TGCTAAAATT TTTAGTTCTT 
GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA CGCGACGGAA TGGCACCGAT ACATGCTGCT 
TCGCAAATGG GATGTTTGGA TTGCCTTAGA TGGATGGTGC AAGATCAGGG TGTTGATCCA 
AATCTACGAG ATGGCGATGG GGCTACACCG CTGCACTTTG CCGCGTCAAG AGGACACCTT 
TCTGTTGTAC GGTGGTTACT CAAACATGGT GCGAAACTAT CATTAGACAA GTATGGCAAG 
TCCCCAATTA ACGATGCTGC AGAAAACCAA CAAGTAGAGT GTCTTAATGT GTTAGTGCAA 
CATGGTAGTA CAATAGATTA TGCTTGTCGT GACAACATCC CACTACAACG ACAGAAAGCA 
TGCTCCTTGT CTAATAAGGA ATTTCGAGAC ATACACAATT GCAATTTCAA TAGTACTGAA 
GGAGGTAACT GTATCAATAG CAGCTCACAC GGGAGCTGTA CCTCGAATAG TAGTAAACAG 
ACTAGTTGCA CCAACACCAT CAAATCAAAA ACTTCTTCGA CTTTAAGCTC AGATATTGAG 
CCCTTTTACT TACATCCCCC ATCGATCTCT ATGGCCCATA AACGCATTGA TGCCATTTAT 
GCACATTCTC AACAATCACG GTCATCGTCT GAGAAATTAT ACAATGGACA AATGGTGCCG 
AATGATGGCT TGTATATAAA TCCAATGCGT AACAGTTTCT TTTCTCCCCC TTCCCCGACT 
GGCAGTGTTT CTGGAGAATC GTTTTTCCTC CACGACCCTC ATGAGCTAAT TTATAATCGA 
GTTCGGGAGT TGTTCGATTC CGATTGTACC AGTATTGAAA ATCAAAAACC GAAAATGAAT 
CGCCACAAAT TTCACAGTCA TAGTAAAATG CATAAATCAA ATACCATAAA CAATGCAGTT 
GTTGTACAAG CGGATGTGCA CTCCAGTTCA AGTGGGACAG GCAGCGGATC AGAAGAATCT 
ATTTCCGCTT CTATTCAATC AAGCATGAAT TTATCCAAAA CAAACAATCT TCGAAGTCAA 
AGTTTTAATC TTCACACTAA ATGTAACAAT GAAATTAGTA GCCACGATCA AGGCTTCAAC 
GCCAGTTCGA GTACAAAGAC CATCACCAAT GATAATAAAA TAATTAATGC AAATTTAACC 
TCTGGAATCA ACTACAATAA AATCAACAAC AATAATATTA AAATTGATAA ACAAAAACAA 
ACAGCTAAGC AATCCAAAAG TAATTATTTA AACAATGAGA AGAAGAAACC AGAAAGTTCA 
CATGACCATG ATTACGAGGA TATATATTTG GTTCGTGAGG AAGCGCGGAA AAACAAAAAC 
AAATACGGTT CCGGGCGATC ACGGTCCCGT GATAGTGGGT CGCATTCACG TTCAGCTAGC 
ACTTCCTCAA CTCGAAGCTC AGACTTCGTA CTACAATATA GCAATCATAA TATGAACAAC 
AAACATCAAA GTGAAGCACT GAATCGAAAT AAATCGCAAT CGACTATTGG AATACATAGC 
AAACCCAAAT ACGAGTTATC GCAAAAGGAA AATTTCACCG TTAAAAATGT GAATCTAAAG 
AACCTGCTGA ACGTCAATAG CAATACCAAT GGTGGTGTTA AAAGCGACAC CTATGAAAGC 
GTGTGTCCGA TAGAAGATAT TGCTGAGCGC ACAAAGCAAT CACAGAAAAA CTCCGTTATT 
CGAAATAATC TTGATTGTGG TAGCATTGGT AACACCAGCA ATATATTCCA CCCAATATGC 
AATGATGTCA GCACATGCGA TCGCCAGTTG AAACGAGTTT CTTCTGCTCC GCCTATACAA 
AACATAGAAA TCGTAAGTGG ACCGCCACCA CCTCCACTGC CGCCACCGAT GAACGCTCCC 
TTACTCAAGA GAATGCAATT AGTTGCATCA GATCCCATGA ACTTTTGCAT TAGTCACTCA 
AATAGCAACT TGGATAATAT AGATTCAGAT TCAGGGCTTG AGGTAATTGA GGAACCTAGT 
TTACGTCCGT CTGAATTGAT CAGAAGCAAA CACAACCGAA CGATGTCTAC CATATCAGCA 
AACAAGAAAG CAAAACTTCT TAATGGCGGA TGTAATGGAA ATATACCCAA CTCTGCTCCA 
ATTGCCAATT GTGTTACACC AAATAGAGAA AACAGTAATT ATCATGATAA AAGCTTTGCT 
TGTGATGGTT ATCACGTTTG CAATGATCAA CAAGATGATA GAGTTCAGGT GCGCAATTAC 
CATTCACACC ATATAGATGT CCAACACAGC TATCAGCAAT CGCAAATGTA TAGATCTTCT 
GCCGAAGATC ACTACGAACT ACAACAGGCA CAGTATGGGT ACAGCAATTC TAATGGGAGC 
ACAGTATGTG GCCCACGTAC CGGTGGTCCA AACTTAGTTA ATAAACAGTT AGTACTTCCC 
TTCGTGCCAC CCAGTTTTCC AAACAAATCA CAAGATGGCG TAACACATCT TATAAAGCCA 
TCTGAATATC TAAAGAGTAT AAGTGATAAG CGATCCTGCC CGTCTTCAGC AAGATCCACA 
GATACTGAAG ATTACATGCA TATCCAGGTG GCTAATCAAC ATGGAGTTAA TTGTGAACCA 
CCAAAGCCTC CACCGCCGCC ACCACTCCCA ATTCATCCGT TAATGCATCA CAATGAAAAA 
CAAAATAGTG TAAAAACAGG AAATATTCAG CATGTATCTG TAGTTAATCA GGATACCGCC 
ACCAGGAAGC AGCATCAACC TCTCTCCGCC ATCTCCATTC AAGATCTTAA TAGTGTTCAG 
CTTCGACGTA CTGATACTCA AAAAATGCCT AAGCCTTACC AAATGCCTGC ACGCAGTTTA 
AGTATGCAGT GTCTTACCAC GTCAGCTGAT TCTTACCTTA AAACGGATTT AATTGCTGAA 
TTAAAGATTT CAAAAGACAT TACGGGCATT AAGAAAATGA AAGTGGAAAA ACAGTTGGCC 
AGTCAATTTG ACACTGAACA TTATTCAGAA ATTTCCAAAC AATTTTCGAC AAACAACTAT 
ATGGAACAGC TTTGTATGCA GATTCCAGAA AAAGATCAGG CAGGTAACAT CATTCCGGAT 
TGGAAACGTC AGATGATGGC GAAAAAGGCA GCCGAACGTG CCAAAAAAGA ATTCGAGGAA 
CGAATGGCAA AAGAAGCAGA AAATAGACGC CTGTCACAAA TACCTCAATG GAAACGTGAC 
CTACTAGCAA GGCGGGAGGA AACCGAAAAT AAATTAAAAG CATCAATTTA TACACCCAAG 
GTGGAGGAAA ATAATCGTAT AGCTGAAACT TGGCATCTTA AGAATCGAGC AATTTCAATT 
GATAACATTA ATTTAGTTTC CCCGAGCGTT GATTCACAGC TACTTGCTTC GGGAACTTTT 
GACAGTAACA AAGAAAATAA GGATCAAAAT GAACAAGCCC TAATAAGTAA CATTCTAACA 
ACGGATGAAG ACATCAATAG TAATCAATAT AGTAAAGGAG ATGACGCCGA TAATATAATA 
CCATGGCGGG CCCAGTTACG AAAAACTAAT AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183447.1
CDS	195..3965
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185145.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105212834 (LOC105212834), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185145.1

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGCGCGAACA CTCAAATGGA CAACGATGTC 
ACACCGGTCT ACTTAGCAGC GCAAGAAGGG CACTTGGAGG TGCTAAAATT TTTAGTTCTT 
GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA CGCGACGGAA TGGCACCGAT ACATGCTGCT 
TCGCAAATGG GATGTTTGGA TTGCCTTAGA TGGATGGTGC AAGATCAGGG TGTTGATCCA 
AATCTACGAG ATGGCGATGG GGCTACACCG CTGCACTTTG CCGCGTCAAG AGGACACCTT 
TCTGTTGTAC GGTGGTTACT CAAACATGGT GCGAAACTAT CATTAGACAA GTATGGCAAG 
TCCCCAATTA ACGATGCTGC AGAAAACCAA CAAGTAGAGT GTCTTAATGT GTTAGTGCAA 
CATGGTAGTA CAATAGATTA TGCTTGTCGT GACAACATCC CACTACAACG ACAGAAAGCA 
TGCTCCTTGT CTAATAAGGA ATTTCGAGAC ATACACAATT GCAATTTCAA TAGTACTGAA 
GGAGGTAACT GTATCAATAG CAGCTCACAC GGGAGCTGTA CCTCGAATAG TAGTAAACAG 
ACTAGTTGCA CCAACACCAT CAAATCAAAA ACTTCTTCGA CTTTAAGCTC AGATATTGAG 
CCCTTTTACT TACATCCCCC ATCGATCTCT ATGGCCCATA AACGCATTGA TGCCATTTAT 
GCACATTCTC AACAATCACG GTCATCGTCT GAGAAATTAT ACAATGGACA AATGGTGCCG 
AATGATGGCT TGTATATAAA TCCAATGCGT AACAGTTTCT TTTCTCCCCC TTCCCCGACT 
GGCAGTGTTT CTGGAGAATC GTTTTTCCTC CACGACCCTC ATGAGCTAAT TTATAATCGA 
GTTCGGGAGT TGTTCGATTC CGATTGTACC AGTATTGAAA ATCAAAAACC GAAAATGAAT 
CGCCACAAAT TTCACAGTCA TAGTAAAATG CATAAATCAA ATACCATAAA CAATGCAGTT 
GTTGTACAAG CGGATGTGCA CTCCAGTTCA AGTGGGACAG GCAGCGGATC AGAAGAATCT 
ATTTCCGCTT CTATTCAATC AAGCATGAAT TTATCCAAAA CAAACAATCT TCGAAGTCAA 
AGTTTTAATC TTCACACTAA ATGTAACAAT GAAATTAGTA GCCACGATCA AGGCTTCAAC 
GCCAGTTCGA GTACAAAGAC CATCACCAAT GATAATAAAA TAATTAATGC AAATTTAACC 
TCTGGAATCA ACTACAATAA AATCAACAAC AATAATATTA AAATTGATAA ACAAAAACAA 
ACAGCTAAGC AATCCAAAAG TAATTATTTA AACAATGAGA AGAAGAAACC AGAAAGTTCA 
CATGACCATG ATTACGAGGA TATATATTTG GTTCGTGAGG AAGCGCGGAA AAACAAAAAC 
AAATACGGTT CCGGGCGATC ACGGTCCCGT GATAGTGGGT CGCATTCACG TTCAGCTAGC 
ACTTCCTCAA CTCGAAGCTC AGACTTCGTA CTACAATATA GCAATCATAA TATGAACAAC 
AAACATCAAA GTGAAGCACT GAATCGAAAT AAATCGCAAT CGACTATTGG AATACATAGC 
AAACCCAAAT ACGAGTTATC GCAAAAGGAA AATTTCACCG TTAAAAATGT GAATCTAAAG 
AACCTGCTGA ACGTCAATAG CAATACCAAT GGTGGTGTTA AAAGCGACAC CTATGAAAGC 
GTGTGTCCGA TAGAAGATAT TGCTGAGCGC ACAAAGCAAT CACAGAAAAA CTCCGTTATT 
CGAAATAATC TTGATTGTGG TAGCATTGGT AACACCAGCA ATATATTCCA CCCAATATGC 
AATGATGTCA GCACATGCGA TCGCCAGTTG AAACGAGTTT CTTCTGCTCC GCCTATACAA 
AACATAGAAA TCGTAAGTGG ACCGCCACCA CCTCCACTGC CGCCACCGAT GAACGCTCCC 
TTACTCAAGA GAATGCAATT AGTTGCATCA GATCCCATGA ACTTTTGCAT TAGTCACTCA 
AATAGCAACT TGGATAATAT AGATTCAGAT TCAGGGCTTG AGGTAATTGA GGAACCTAGT 
TTACGTCCGT CTGAATTGAT CAGAAGCAAA CACAACCGAA CGATGTCTAC CATATCAGCA 
AACAAGAAAG CAAAACTTCT TAATGGCGGA TGTAATGGAA ATATACCCAA CTCTGCTCCA 
ATTGCCAATT GTGTTACACC AAATAGAGAA AACAGTAATT ATCATGATAA AAGCTTTGCT 
TGTGATGGTT ATCACGTTTG CAATGATCAA CAAGATGATA GAGTTCAGGT GCGCAATTAC 
CATTCACACC ATATAGATGT CCAACACAGC TATCAGCAAT CGCAAATGTA TAGATCTTCT 
GCCGAAGATC ACTACGAACT ACAACAGGCA CAGTATGGGT ACAGCAATTC TAATGGGAGC 
ACAGTATGTG GCCCACGTAC CGGTGGTCCA AACTTAGTTA ATAAACAGTT AGTACTTCCC 
TTCGTGCCAC CCAGTTTTCC AAACAAATCA CAAGATGGCG TAACACATCT TATAAAGCCA 
TCTGAATATC TAAAGAGTAT AAGTGATAAG CGATCCTGCC CGTCTTCAGC AAGATCCACA 
GATACTGAAG ATTACATGCA TATCCAGGTG GCTAATCAAC ATGGAGTTAA TTGTGAACCA 
CCAAAGCCTC CACCGCCGCC ACCACTCCCA ATTCATCCGT TAATGCATCA CAATGAAAAA 
CAAAATAGTG TAAAAACAGG AAATATTCAG CATGTATCTG TAGTTAATCA GGATACCGCC 
ACCAGGAAGC AGCATCAACC TCTCTCCGCC ATCTCCATTC AAGATCTTAA TAGTGTTCAG 
CTTCGACGTA CTGATACTCA AAAAATGCCT AAGCCTTACC AAATGCCTGC ACGCAGTTTA 
AGTATGCAGT GTCTTACCAC GTCAGCTGAT TCTTACCTTA AAACGGATTT AATTGCTGAA 
TTAAAGATTT CAAAAGACAT TACGGGCATT AAGAAAATGA AAGTGGAAAA ACAGTTGGCC 
AGTCAATTTG ACACTGAACA TTATTCAGAA ATTTCCAAAC AATTTTCGAC AAACAACTAT 
ATGGAACAGC TTTGTATGCA GATTCCAGAA AAAGATCAGG CAGGTAACAT CATTCCGGAT 
TGGAAACGTC AGATGATGGC GAAAAAGGCA GCCGAACGTG CCAAAAAAGA ATTCGAGGAA 
CGAATGGCAA AAGAAGCAGA AAATAGACGC CTGTCACAAA TACCTCAATG GAAACGTGAC 
CTACTAGCAA GGCGGGAGGA AACCGAAAAT AAATTAAAAG CATCAATTTA TACACCCAAG 
GTGGAGGAAA ATAATCGTAT AGCTGAAACT TGGCATCTTA AGAATCGAGC AATTTCAATT 
GATAACATTA ATTTAGTTTC CCCGAGCGTT GATTCACAGC TACTTGCTTC GGGAACTTTT 
GACAGTAACA AAGAAAATAA GGATCAAAAT GAACAAGCCC TAATAAGTAA CATTCTAACA 
ACGGATGAAG ACATCAATAG TAATCAATAT AGTAAAGGAG ATGACGCCGA TAATATAATA 
CCATGGCGGG CCCAGTTACG AAAAACTAAT AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185145.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183447.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X6, mRNA.

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CloneID	OBa199154
Clone ID Related Accession (Same CDS sequence)	XM_011185145.1 , XM_011185145.2
Accession Version	XM_011185145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011185145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGCGCGAACA CTCAAATGGA CAACGATGTC 
ACACCGGTCT ACTTAGCAGC GCAAGAAGGG CACTTGGAGG TGCTAAAATT TTTAGTTCTT 
GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA CGCGACGGAA TGGCACCGAT ACATGCTGCT 
TCGCAAATGG GATGTTTGGA TTGCCTTAGA TGGATGGTGC AAGATCAGGG TGTTGATCCA 
AATCTACGAG ATGGCGATGG GGCTACACCG CTGCACTTTG CCGCGTCAAG AGGACACCTT 
TCTGTTGTAC GGTGGTTACT CAAACATGGT GCGAAACTAT CATTAGACAA GTATGGCAAG 
TCCCCAATTA ACGATGCTGC AGAAAACCAA CAAGTAGAGT GTCTTAATGT GTTAGTGCAA 
CATGGTAGTA CAATAGATTA TGCTTGTCGT GACAACATCC CACTACAACG ACAGAAAGCA 
TGCTCCTTGT CTAATAAGGA ATTTCGAGAC ATACACAATT GCAATTTCAA TAGTACTGAA 
GGAGGTAACT GTATCAATAG CAGCTCACAC GGGAGCTGTA CCTCGAATAG TAGTAAACAG 
ACTAGTTGCA CCAACACCAT CAAATCAAAA ACTTCTTCGA CTTTAAGCTC AGATATTGAG 
CCCTTTTACT TACATCCCCC ATCGATCTCT ATGGCCCATA AACGCATTGA TGCCATTTAT 
GCACATTCTC AACAATCACG GTCATCGTCT GAGAAATTAT ACAATGGACA AATGGTGCCG 
AATGATGGCT TGTATATAAA TCCAATGCGT AACAGTTTCT TTTCTCCCCC TTCCCCGACT 
GGCAGTGTTT CTGGAGAATC GTTTTTCCTC CACGACCCTC ATGAGCTAAT TTATAATCGA 
GTTCGGGAGT TGTTCGATTC CGATTGTACC AGTATTGAAA ATCAAAAACC GAAAATGAAT 
CGCCACAAAT TTCACAGTCA TAGTAAAATG CATAAATCAA ATACCATAAA CAATGCAGTT 
GTTGTACAAG CGGATGTGCA CTCCAGTTCA AGTGGGACAG GCAGCGGATC AGAAGAATCT 
ATTTCCGCTT CTATTCAATC AAGCATGAAT TTATCCAAAA CAAACAATCT TCGAAGTCAA 
AGTTTTAATC TTCACACTAA ATGTAACAAT GAAATTAGTA GCCACGATCA AGGCTTCAAC 
GCCAGTTCGA GTACAAAGAC CATCACCAAT GATAATAAAA TAATTAATGC AAATTTAACC 
TCTGGAATCA ACTACAATAA AATCAACAAC AATAATATTA AAATTGATAA ACAAAAACAA 
ACAGCTAAGC AATCCAAAAG TAATTATTTA AACAATGAGA AGAAGAAACC AGAAAGTTCA 
CATGACCATG ATTACGAGGA TATATATTTG GTTCGTGAGG AAGCGCGGAA AAACAAAAAC 
AAATACGGTT CCGGGCGATC ACGGTCCCGT GATAGTGGGT CGCATTCACG TTCAGCTAGC 
ACTTCCTCAA CTCGAAGCTC AGACTTCGTA CTACAATATA GCAATCATAA TATGAACAAC 
AAACATCAAA GTGAAGCACT GAATCGAAAT AAATCGCAAT CGACTATTGG AATACATAGC 
AAACCCAAAT ACGAGTTATC GCAAAAGGAA AATTTCACCG TTAAAAATGT GAATCTAAAG 
AACCTGCTGA ACGTCAATAG CAATACCAAT GGTGGTGTTA AAAGCGACAC CTATGAAAGC 
GTGTGTCCGA TAGAAGATAT TGCTGAGCGC ACAAAGCAAT CACAGAAAAA CTCCGTTATT 
CGAAATAATC TTGATTGTGG TAGCATTGGT AACACCAGCA ATATATTCCA CCCAATATGC 
AATGATGTCA GCACATGCGA TCGCCAGTTG AAACGAGTTT CTTCTGCTCC GCCTATACAA 
AACATAGAAA TCGTAAGTGG ACCGCCACCA CCTCCACTGC CGCCACCGAT GAACGCTCCC 
TTACTCAAGA GAATGCAATT AGTTGCATCA GATCCCATGA ACTTTTGCAT TAGTCACTCA 
AATAGCAACT TGGATAATAT AGATTCAGAT TCAGGGCTTG AGGTAATTGA GGAACCTAGT 
TTACGTCCGT CTGAATTGAT CAGAAGCAAA CACAACCGAA CGATGTCTAC CATATCAGCA 
AACAAGAAAG CAAAACTTCT TAATGGCGGA TGTAATGGAA ATATACCCAA CTCTGCTCCA 
ATTGCCAATT GTGTTACACC AAATAGAGAA AACAGTAATT ATCATGATAA AAGCTTTGCT 
TGTGATGGTT ATCACGTTTG CAATGATCAA CAAGATGATA GAGTTCAGGT GCGCAATTAC 
CATTCACACC ATATAGATGT CCAACACAGC TATCAGCAAT CGCAAATGTA TAGATCTTCT 
GCCGAAGATC ACTACGAACT ACAACAGGCA CAGTATGGGT ACAGCAATTC TAATGGGAGC 
ACAGTATGTG GCCCACGTAC CGGTGGTCCA AACTTAGTTA ATAAACAGTT AGTACTTCCC 
TTCGTGCCAC CCAGTTTTCC AAACAAATCA CAAGATGGCG TAACACATCT TATAAAGCCA 
TCTGAATATC TAAAGAGTAT AAGTGATAAG CGATCCTGCC CGTCTTCAGC AAGATCCACA 
GATACTGAAG ATTACATGCA TATCCAGGTG GCTAATCAAC ATGGAGTTAA TTGTGAACCA 
CCAAAGCCTC CACCGCCGCC ACCACTCCCA ATTCATCCGT TAATGCATCA CAATGAAAAA 
CAAAATAGTG TAAAAACAGG AAATATTCAG CATGTATCTG TAGTTAATCA GGATACCGCC 
ACCAGGAAGC AGCATCAACC TCTCTCCGCC ATCTCCATTC AAGATCTTAA TAGTGTTCAG 
CTTCGACGTA CTGATACTCA AAAAATGCCT AAGCCTTACC AAATGCCTGC ACGCAGTTTA 
AGTATGCAGT GTCTTACCAC GTCAGCTGAT TCTTACCTTA AAACGGATTT AATTGCTGAA 
TTAAAGATTT CAAAAGACAT TACGGGCATT AAGAAAATGA AAGTGGAAAA ACAGTTGGCC 
AGTCAATTTG ACACTGAACA TTATTCAGAA ATTTCCAAAC AATTTTCGAC AAACAACTAT 
ATGGAACAGC TTTGTATGCA GATTCCAGAA AAAGATCAGG CAGGTAACAT CATTCCGGAT 
TGGAAACGTC AGATGATGGC GAAAAAGGCA GCCGAACGTG CCAAAAAAGA ATTCGAGGAA 
CGAATGGCAA AAGAAGCAGA AAATAGACGC CTGTCACAAA TACCTCAATG GAAACGTGAC 
CTACTAGCAA GGCGGGAGGA AACCGAAAAT AAATTAAAAG CATCAATTTA TACACCCAAG 
GTGGAGGAAA ATAATCGTAT AGCTGAAACT TGGCATCTTA AGAATCGAGC AATTTCAATT 
GATAACATTA ATTTAGTTTC CCCGAGCGTT GATTCACAGC TACTTGCTTC GGGAACTTTT 
GACAGTAACA AAGAAAATAA GGATCAAAAT GAACAAGCCC TAATAAGTAA CATTCTAACA 
ACGGATGAAG ACATCAATAG TAATCAATAT AGTAAAGGAG ATGACGCCGA TAATATAATA 
CCATGGCGGG CCCAGTTACG AAAAACTAAT AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183447.1
CDS	421..4191
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQLCMQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185145.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185145.2

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGCGCGAACA CTCAAATGGA CAACGATGTC 
ACACCGGTCT ACTTAGCAGC GCAAGAAGGG CACTTGGAGG TGCTAAAATT TTTAGTTCTT 
GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA CGCGACGGAA TGGCACCGAT ACATGCTGCT 
TCGCAAATGG GATGTTTGGA TTGCCTTAGA TGGATGGTGC AAGATCAGGG TGTTGATCCA 
AATCTACGAG ATGGCGATGG GGCTACACCG CTGCACTTTG CCGCGTCAAG AGGACACCTT 
TCTGTTGTAC GGTGGTTACT CAAACATGGT GCGAAACTAT CATTAGACAA GTATGGCAAG 
TCCCCAATTA ACGATGCTGC AGAAAACCAA CAAGTAGAGT GTCTTAATGT GTTAGTGCAA 
CATGGTAGTA CAATAGATTA TGCTTGTCGT GACAACATCC CACTACAACG ACAGAAAGCA 
TGCTCCTTGT CTAATAAGGA ATTTCGAGAC ATACACAATT GCAATTTCAA TAGTACTGAA 
GGAGGTAACT GTATCAATAG CAGCTCACAC GGGAGCTGTA CCTCGAATAG TAGTAAACAG 
ACTAGTTGCA CCAACACCAT CAAATCAAAA ACTTCTTCGA CTTTAAGCTC AGATATTGAG 
CCCTTTTACT TACATCCCCC ATCGATCTCT ATGGCCCATA AACGCATTGA TGCCATTTAT 
GCACATTCTC AACAATCACG GTCATCGTCT GAGAAATTAT ACAATGGACA AATGGTGCCG 
AATGATGGCT TGTATATAAA TCCAATGCGT AACAGTTTCT TTTCTCCCCC TTCCCCGACT 
GGCAGTGTTT CTGGAGAATC GTTTTTCCTC CACGACCCTC ATGAGCTAAT TTATAATCGA 
GTTCGGGAGT TGTTCGATTC CGATTGTACC AGTATTGAAA ATCAAAAACC GAAAATGAAT 
CGCCACAAAT TTCACAGTCA TAGTAAAATG CATAAATCAA ATACCATAAA CAATGCAGTT 
GTTGTACAAG CGGATGTGCA CTCCAGTTCA AGTGGGACAG GCAGCGGATC AGAAGAATCT 
ATTTCCGCTT CTATTCAATC AAGCATGAAT TTATCCAAAA CAAACAATCT TCGAAGTCAA 
AGTTTTAATC TTCACACTAA ATGTAACAAT GAAATTAGTA GCCACGATCA AGGCTTCAAC 
GCCAGTTCGA GTACAAAGAC CATCACCAAT GATAATAAAA TAATTAATGC AAATTTAACC 
TCTGGAATCA ACTACAATAA AATCAACAAC AATAATATTA AAATTGATAA ACAAAAACAA 
ACAGCTAAGC AATCCAAAAG TAATTATTTA AACAATGAGA AGAAGAAACC AGAAAGTTCA 
CATGACCATG ATTACGAGGA TATATATTTG GTTCGTGAGG AAGCGCGGAA AAACAAAAAC 
AAATACGGTT CCGGGCGATC ACGGTCCCGT GATAGTGGGT CGCATTCACG TTCAGCTAGC 
ACTTCCTCAA CTCGAAGCTC AGACTTCGTA CTACAATATA GCAATCATAA TATGAACAAC 
AAACATCAAA GTGAAGCACT GAATCGAAAT AAATCGCAAT CGACTATTGG AATACATAGC 
AAACCCAAAT ACGAGTTATC GCAAAAGGAA AATTTCACCG TTAAAAATGT GAATCTAAAG 
AACCTGCTGA ACGTCAATAG CAATACCAAT GGTGGTGTTA AAAGCGACAC CTATGAAAGC 
GTGTGTCCGA TAGAAGATAT TGCTGAGCGC ACAAAGCAAT CACAGAAAAA CTCCGTTATT 
CGAAATAATC TTGATTGTGG TAGCATTGGT AACACCAGCA ATATATTCCA CCCAATATGC 
AATGATGTCA GCACATGCGA TCGCCAGTTG AAACGAGTTT CTTCTGCTCC GCCTATACAA 
AACATAGAAA TCGTAAGTGG ACCGCCACCA CCTCCACTGC CGCCACCGAT GAACGCTCCC 
TTACTCAAGA GAATGCAATT AGTTGCATCA GATCCCATGA ACTTTTGCAT TAGTCACTCA 
AATAGCAACT TGGATAATAT AGATTCAGAT TCAGGGCTTG AGGTAATTGA GGAACCTAGT 
TTACGTCCGT CTGAATTGAT CAGAAGCAAA CACAACCGAA CGATGTCTAC CATATCAGCA 
AACAAGAAAG CAAAACTTCT TAATGGCGGA TGTAATGGAA ATATACCCAA CTCTGCTCCA 
ATTGCCAATT GTGTTACACC AAATAGAGAA AACAGTAATT ATCATGATAA AAGCTTTGCT 
TGTGATGGTT ATCACGTTTG CAATGATCAA CAAGATGATA GAGTTCAGGT GCGCAATTAC 
CATTCACACC ATATAGATGT CCAACACAGC TATCAGCAAT CGCAAATGTA TAGATCTTCT 
GCCGAAGATC ACTACGAACT ACAACAGGCA CAGTATGGGT ACAGCAATTC TAATGGGAGC 
ACAGTATGTG GCCCACGTAC CGGTGGTCCA AACTTAGTTA ATAAACAGTT AGTACTTCCC 
TTCGTGCCAC CCAGTTTTCC AAACAAATCA CAAGATGGCG TAACACATCT TATAAAGCCA 
TCTGAATATC TAAAGAGTAT AAGTGATAAG CGATCCTGCC CGTCTTCAGC AAGATCCACA 
GATACTGAAG ATTACATGCA TATCCAGGTG GCTAATCAAC ATGGAGTTAA TTGTGAACCA 
CCAAAGCCTC CACCGCCGCC ACCACTCCCA ATTCATCCGT TAATGCATCA CAATGAAAAA 
CAAAATAGTG TAAAAACAGG AAATATTCAG CATGTATCTG TAGTTAATCA GGATACCGCC 
ACCAGGAAGC AGCATCAACC TCTCTCCGCC ATCTCCATTC AAGATCTTAA TAGTGTTCAG 
CTTCGACGTA CTGATACTCA AAAAATGCCT AAGCCTTACC AAATGCCTGC ACGCAGTTTA 
AGTATGCAGT GTCTTACCAC GTCAGCTGAT TCTTACCTTA AAACGGATTT AATTGCTGAA 
TTAAAGATTT CAAAAGACAT TACGGGCATT AAGAAAATGA AAGTGGAAAA ACAGTTGGCC 
AGTCAATTTG ACACTGAACA TTATTCAGAA ATTTCCAAAC AATTTTCGAC AAACAACTAT 
ATGGAACAGC TTTGTATGCA GATTCCAGAA AAAGATCAGG CAGGTAACAT CATTCCGGAT 
TGGAAACGTC AGATGATGGC GAAAAAGGCA GCCGAACGTG CCAAAAAAGA ATTCGAGGAA 
CGAATGGCAA AAGAAGCAGA AAATAGACGC CTGTCACAAA TACCTCAATG GAAACGTGAC 
CTACTAGCAA GGCGGGAGGA AACCGAAAAT AAATTAAAAG CATCAATTTA TACACCCAAG 
GTGGAGGAAA ATAATCGTAT AGCTGAAACT TGGCATCTTA AGAATCGAGC AATTTCAATT 
GATAACATTA ATTTAGTTTC CCCGAGCGTT GATTCACAGC TACTTGCTTC GGGAACTTTT 
GACAGTAACA AAGAAAATAA GGATCAAAAT GAACAAGCCC TAATAAGTAA CATTCTAACA 
ACGGATGAAG ACATCAATAG TAATCAATAT AGTAAAGGAG ATGACGCCGA TAATATAATA 
CCATGGCGGG CCCAGTTACG AAAAACTAAT AGTAGACTAA GTTTAATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_011185146.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183448.1 Zeugodacus cucurbitae Espin (Espn), transcript variant X7, mRNA.

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CloneID	OBa199155
Clone ID Related Accession (Same CDS sequence)	XM_011185146.1
Accession Version	XM_011185146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein Espn isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863709.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGCGCGAACA CTCAAATGGA CAACGATGTC 
ACACCGGTCT ACTTAGCAGC GCAAGAAGGG CACTTGGAGG TGCTAAAATT TTTAGTTCTT 
GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA CGCGACGGAA TGGCACCGAT ACATGCTGCT 
TCGCAAATGG GATGTTTGGA TTGCCTTAGA TGGATGGTGC AAGATCAGGG TGTTGATCCA 
AATCTACGAG ATGGCGATGG GGCTACACCG CTGCACTTTG CCGCGTCAAG AGGACACCTT 
TCTGTTGTAC GGTGGTTACT CAAACATGGT GCGAAACTAT CATTAGACAA GTATGGCAAG 
TCCCCAATTA ACGATGCTGC AGAAAACCAA CAAGTAGAGT GTCTTAATGT GTTAGTGCAA 
CATGGTAGTA CAATAGATTA TGCTTGTCGT GACAACATCC CACTACAACG ACAGAAAGCA 
TGCTCCTTGT CTAATAAGGA ATTTCGAGAC ATACACAATT GCAATTTCAA TAGTACTGAA 
GGAGGTAACT GTATCAATAG CAGCTCACAC GGGAGCTGTA CCTCGAATAG TAGTAAACAG 
ACTAGTTGCA CCAACACCAT CAAATCAAAA ACTTCTTCGA CTTTAAGCTC AGATATTGAG 
CCCTTTTACT TACATCCCCC ATCGATCTCT ATGGCCCATA AACGCATTGA TGCCATTTAT 
GCACATTCTC AACAATCACG GTCATCGTCT GAGAAATTAT ACAATGGACA AATGGTGCCG 
AATGATGGCT TGTATATAAA TCCAATGCGT AACAGTTTCT TTTCTCCCCC TTCCCCGACT 
GGCAGTGTTT CTGGAGAATC GTTTTTCCTC CACGACCCTC ATGAGCTAAT TTATAATCGA 
GTTCGGGAGT TGTTCGATTC CGATTGTACC AGTATTGAAA ATCAAAAACC GAAAATGAAT 
CGCCACAAAT TTCACAGTCA TAGTAAAATG CATAAATCAA ATACCATAAA CAATGCAGTT 
GTTGTACAAG CGGATGTGCA CTCCAGTTCA AGTGGGACAG GCAGCGGATC AGAAGAATCT 
ATTTCCGCTT CTATTCAATC AAGCATGAAT TTATCCAAAA CAAACAATCT TCGAAGTCAA 
AGTTTTAATC TTCACACTAA ATGTAACAAT GAAATTAGTA GCCACGATCA AGGCTTCAAC 
GCCAGTTCGA GTACAAAGAC CATCACCAAT GATAATAAAA TAATTAATGC AAATTTAACC 
TCTGGAATCA ACTACAATAA AATCAACAAC AATAATATTA AAATTGATAA ACAAAAACAA 
ACAGCTAAGC AATCCAAAAG TAATTATTTA AACAATGAGA AGAAGAAACC AGAAAGTTCA 
CATGACCATG ATTACGAGGA TATATATTTG GTTCGTGAGG AAGCGCGGAA AAACAAAAAC 
AAATACGGTT CCGGGCGATC ACGGTCCCGT GATAGTGGGT CGCATTCACG TTCAGCTAGC 
ACTTCCTCAA CTCGAAGCTC AGACTTCGTA CTACAATATA GCAATCATAA TATGAACAAC 
AAACATCAAA GTGAAGCACT GAATCGAAAT AAATCGCAAT CGACTATTGG AATACATAGC 
AAACCCAAAT ACGAGTTATC GCAAAAGGAA AATTTCACCG TTAAAAATGT GAATCTAAAG 
AACCTGCTGA ACGTCAATAG CAATACCAAT GGTGGTGTTA AAAGCGACAC CTATGAAAGC 
GTGTGTCCGA TAGAAGATAT TGCTGAGCGC ACAAAGCAAT CACAGAAAAA CTCCGTTATT 
CGAAATAATC TTGATTGTGG TAGCATTGGT AACACCAGCA ATATATTCCA CCCAATATGC 
AATGATGTCA GCACATGCGA TCGCCAGTTG AAACGAGTTT CTTCTGCTCC GCCTATACAA 
AACATAGAAA TCGTAAGTGG ACCGCCACCA CCTCCACTGC CGCCACCGAT GAACGCTCCC 
TTACTCAAGA GAATGCAATT AGTTGCATCA GATCCCATGA ACTTTTGCAT TAGTCACTCA 
AATAGCAACT TGGATAATAT AGATTCAGAT TCAGGGCTTG AGGTAATTGA GGAACCTAGT 
TTACGTCCGT CTGAATTGAT CAGAAGCAAA CACAACCGAA CGATGTCTAC CATATCAGCA 
AACAAGAAAG CAAAACTTCT TAATGGCGGA TGTAATGGAA ATATACCCAA CTCTGCTCCA 
ATTGCCAATT GTGTTACACC AAATAGAGAA AACAGTAATT ATCATGATAA AAGCTTTGCT 
TGTGATGGTT ATCACGTTTG CAATGATCAA CAAGATGATA GAGTTCAGGT GCGCAATTAC 
CATTCACACC ATATAGATGT CCAACACAGC TATCAGCAAT CGCAAATGTA TAGATCTTCT 
GCCGAAGATC ACTACGAACT ACAACAGGCA CAGTATGGGT ACAGCAATTC TAATGGGAGC 
ACAGTATGTG GCCCACGTAC CGGTGGTCCA AACTTAGTTA ATAAACAGTT AGTACTTCCC 
TTCGTGCCAC CCAGTTTTCC AAACAAATCA CAAGATGGCG TAACACATCT TATAAAGCCA 
TCTGAATATC TAAAGAGTAT AAGTGATAAG CGATCCTGCC CGTCTTCAGC AAGATCCACA 
GATACTGAAG ATTACATGCA TATCCAGGTG GCTAATCAAC ATGGAGTTAA TTGTGAACCA 
CCAAAGCCTC CACCGCCGCC ACCACTCCCA ATTCATCCGT TAATGCATCA CAATGAAAAA 
CAAAATAGTG TAAAAACAGG AAATATTCAG CATGTATCTG TAGTTAATCA GGATACCGCC 
ACCAGGAAGC AGCATCAACC TCTCTCCGCC ATCTCCATTC AAGATCTTAA TAGTGTTCAG 
CTTCGACGTA CTGATACTCA AAAAATGCCT AAGCCTTACC AAATGCCTGC ACGCAGTTTA 
AGTATGCAGT GTCTTACCAC GTCAGCTGAT TCTTACCTTA AAACGGATTT AATTGCTGAA 
TTAAAGATTT CAAAAGACAT TACGGGCATT AAGAAAATGA AAGTGGAAAA ACAGTTGGCC 
AGTCAATTTG ACACTGAACA TTATTCAGAA ATTTCCAAAC AATTTTCGAC AAACAACTAT 
ATGGAACAGA TTCCAGAAAA AGATCAGGCA GGTAACATCA TTCCGGATTG GAAACGTCAG 
ATGATGGCGA AAAAGGCAGC CGAACGTGCC AAAAAAGAAT TCGAGGAACG AATGGCAAAA 
GAAGCAGAAA ATAGACGCCT GTCACAAATA CCTCAATGGA AACGTGACCT ACTAGCAAGG 
CGGGAGGAAA CCGAAAATAA ATTAAAAGCA TCAATTTATA CACCCAAGGT GGAGGAAAAT 
AATCGTATAG CTGAAACTTG GCATCTTAAG AATCGAGCAA TTTCAATTGA TAACATTAAT 
TTAGTTTCCC CGAGCGTTGA TTCACAGCTA CTTGCTTCGG GAACTTTTGA CAGTAACAAA 
GAAAATAAGG ATCAAAATGA ACAAGCCCTA ATAAGTAACA TTCTAACAAC GGATGAAGAC 
ATCAATAGTA ATCAATATAG TAAAGGAGAT GACGCCGATA ATATAATACC ATGGCGGGCC 
CAGTTACGAA AAACTAATAG TAGACTAAGT TTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183448.1
CDS	74..3832
Translation	MHSHKLNTCGSTGSISSIERPNAGALALHYAAARGCLDCVQLLVRASPDICANTQMDNDVTPVYLAAQEGHLEVLKFLVLEAGGSLYVRARDGMAPIHAASQMGCLDCLRWMVQDQGVDPNLRDGDGATPLHFAASRGHLSVVRWLLKHGAKLSLDKYGKSPINDAAENQQVECLNVLVQHGSTIDYACRDNIPLQRQKACSLSNKEFRDIHNCNFNSTEGGNCINSSSHGSCTSNSSKQTSCTNTIKSKTSSTLSSDIEPFYLHPPSISMAHKRIDAIYAHSQQSRSSSEKLYNGQMVPNDGLYINPMRNSFFSPPSPTGSVSGESFFLHDPHELIYNRVRELFDSDCTSIENQKPKMNRHKFHSHSKMHKSNTINNAVVVQADVHSSSSGTGSGSEESISASIQSSMNLSKTNNLRSQSFNLHTKCNNEISSHDQGFNASSSTKTITNDNKIINANLTSGINYNKINNNNIKIDKQKQTAKQSKSNYLNNEKKKPESSHDHDYEDIYLVREEARKNKNKYGSGRSRSRDSGSHSRSASTSSTRSSDFVLQYSNHNMNNKHQSEALNRNKSQSTIGIHSKPKYELSQKENFTVKNVNLKNLLNVNSNTNGGVKSDTYESVCPIEDIAERTKQSQKNSVIRNNLDCGSIGNTSNIFHPICNDVSTCDRQLKRVSSAPPIQNIEIVSGPPPPPLPPPMNAPLLKRMQLVASDPMNFCISHSNSNLDNIDSDSGLEVIEEPSLRPSELIRSKHNRTMSTISANKKAKLLNGGCNGNIPNSAPIANCVTPNRENSNYHDKSFACDGYHVCNDQQDDRVQVRNYHSHHIDVQHSYQQSQMYRSSAEDHYELQQAQYGYSNSNGSTVCGPRTGGPNLVNKQLVLPFVPPSFPNKSQDGVTHLIKPSEYLKSISDKRSCPSSARSTDTEDYMHIQVANQHGVNCEPPKPPPPPPLPIHPLMHHNEKQNSVKTGNIQHVSVVNQDTATRKQHQPLSAISIQDLNSVQLRRTDTQKMPKPYQMPARSLSMQCLTTSADSYLKTDLIAELKISKDITGIKKMKVEKQLASQFDTEHYSEISKQFSTNNYMEQIPEKDQAGNIIPDWKRQMMAKKAAERAKKEFEERMAKEAENRRLSQIPQWKRDLLARREETENKLKASIYTPKVEENNRIAETWHLKNRAISIDNINLVSPSVDSQLLASGTFDSNKENKDQNEQALISNILTTDEDINSNQYSKGDDADNIIPWRAQLRKTNSRLSLI

Target ORF information:

RefSeq Version	XM_011185146.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae Espin (Espn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011185146.1

ORF Insert Sequence:

ATGCATTCGC ATAAATTAAA CACATGTGGC TCAACGGGGA GCATTTCATC AATAGAAAGA 
CCAAATGCGG GCGCATTGGC ATTGCATTAT GCCGCAGCAC GCGGGTGTTT AGACTGTGTA 
CAATTGCTGG TTCGAGCATC ACCAGATATT TGCGCGAACA CTCAAATGGA CAACGATGTC 
ACACCGGTCT ACTTAGCAGC GCAAGAAGGG CACTTGGAGG TGCTAAAATT TTTAGTTCTT 
GAAGCTGGCG GTTCTCTCTA TGTGCGAGCA CGCGACGGAA TGGCACCGAT ACATGCTGCT 
TCGCAAATGG GATGTTTGGA TTGCCTTAGA TGGATGGTGC AAGATCAGGG TGTTGATCCA 
AATCTACGAG ATGGCGATGG GGCTACACCG CTGCACTTTG CCGCGTCAAG AGGACACCTT 
TCTGTTGTAC GGTGGTTACT CAAACATGGT GCGAAACTAT CATTAGACAA GTATGGCAAG 
TCCCCAATTA ACGATGCTGC AGAAAACCAA CAAGTAGAGT GTCTTAATGT GTTAGTGCAA 
CATGGTAGTA CAATAGATTA TGCTTGTCGT GACAACATCC CACTACAACG ACAGAAAGCA 
TGCTCCTTGT CTAATAAGGA ATTTCGAGAC ATACACAATT GCAATTTCAA TAGTACTGAA 
GGAGGTAACT GTATCAATAG CAGCTCACAC GGGAGCTGTA CCTCGAATAG TAGTAAACAG 
ACTAGTTGCA CCAACACCAT CAAATCAAAA ACTTCTTCGA CTTTAAGCTC AGATATTGAG 
CCCTTTTACT TACATCCCCC ATCGATCTCT ATGGCCCATA AACGCATTGA TGCCATTTAT 
GCACATTCTC AACAATCACG GTCATCGTCT GAGAAATTAT ACAATGGACA AATGGTGCCG 
AATGATGGCT TGTATATAAA TCCAATGCGT AACAGTTTCT TTTCTCCCCC TTCCCCGACT 
GGCAGTGTTT CTGGAGAATC GTTTTTCCTC CACGACCCTC ATGAGCTAAT TTATAATCGA 
GTTCGGGAGT TGTTCGATTC CGATTGTACC AGTATTGAAA ATCAAAAACC GAAAATGAAT 
CGCCACAAAT TTCACAGTCA TAGTAAAATG CATAAATCAA ATACCATAAA CAATGCAGTT 
GTTGTACAAG CGGATGTGCA CTCCAGTTCA AGTGGGACAG GCAGCGGATC AGAAGAATCT 
ATTTCCGCTT CTATTCAATC AAGCATGAAT TTATCCAAAA CAAACAATCT TCGAAGTCAA 
AGTTTTAATC TTCACACTAA ATGTAACAAT GAAATTAGTA GCCACGATCA AGGCTTCAAC 
GCCAGTTCGA GTACAAAGAC CATCACCAAT GATAATAAAA TAATTAATGC AAATTTAACC 
TCTGGAATCA ACTACAATAA AATCAACAAC AATAATATTA AAATTGATAA ACAAAAACAA 
ACAGCTAAGC AATCCAAAAG TAATTATTTA AACAATGAGA AGAAGAAACC AGAAAGTTCA 
CATGACCATG ATTACGAGGA TATATATTTG GTTCGTGAGG AAGCGCGGAA AAACAAAAAC 
AAATACGGTT CCGGGCGATC ACGGTCCCGT GATAGTGGGT CGCATTCACG TTCAGCTAGC 
ACTTCCTCAA CTCGAAGCTC AGACTTCGTA CTACAATATA GCAATCATAA TATGAACAAC 
AAACATCAAA GTGAAGCACT GAATCGAAAT AAATCGCAAT CGACTATTGG AATACATAGC 
AAACCCAAAT ACGAGTTATC GCAAAAGGAA AATTTCACCG TTAAAAATGT GAATCTAAAG 
AACCTGCTGA ACGTCAATAG CAATACCAAT GGTGGTGTTA AAAGCGACAC CTATGAAAGC 
GTGTGTCCGA TAGAAGATAT TGCTGAGCGC ACAAAGCAAT CACAGAAAAA CTCCGTTATT 
CGAAATAATC TTGATTGTGG TAGCATTGGT AACACCAGCA ATATATTCCA CCCAATATGC 
AATGATGTCA GCACATGCGA TCGCCAGTTG AAACGAGTTT CTTCTGCTCC GCCTATACAA 
AACATAGAAA TCGTAAGTGG ACCGCCACCA CCTCCACTGC CGCCACCGAT GAACGCTCCC 
TTACTCAAGA GAATGCAATT AGTTGCATCA GATCCCATGA ACTTTTGCAT TAGTCACTCA 
AATAGCAACT TGGATAATAT AGATTCAGAT TCAGGGCTTG AGGTAATTGA GGAACCTAGT 
TTACGTCCGT CTGAATTGAT CAGAAGCAAA CACAACCGAA CGATGTCTAC CATATCAGCA 
AACAAGAAAG CAAAACTTCT TAATGGCGGA TGTAATGGAA ATATACCCAA CTCTGCTCCA 
ATTGCCAATT GTGTTACACC AAATAGAGAA AACAGTAATT ATCATGATAA AAGCTTTGCT 
TGTGATGGTT ATCACGTTTG CAATGATCAA CAAGATGATA GAGTTCAGGT GCGCAATTAC 
CATTCACACC ATATAGATGT CCAACACAGC TATCAGCAAT CGCAAATGTA TAGATCTTCT 
GCCGAAGATC ACTACGAACT ACAACAGGCA CAGTATGGGT ACAGCAATTC TAATGGGAGC 
ACAGTATGTG GCCCACGTAC CGGTGGTCCA AACTTAGTTA ATAAACAGTT AGTACTTCCC 
TTCGTGCCAC CCAGTTTTCC AAACAAATCA CAAGATGGCG TAACACATCT TATAAAGCCA 
TCTGAATATC TAAAGAGTAT AAGTGATAAG CGATCCTGCC CGTCTTCAGC AAGATCCACA 
GATACTGAAG ATTACATGCA TATCCAGGTG GCTAATCAAC ATGGAGTTAA TTGTGAACCA 
CCAAAGCCTC CACCGCCGCC ACCACTCCCA ATTCATCCGT TAATGCATCA CAATGAAAAA 
CAAAATAGTG TAAAAACAGG AAATATTCAG CATGTATCTG TAGTTAATCA GGATACCGCC 
ACCAGGAAGC AGCATCAACC TCTCTCCGCC ATCTCCATTC AAGATCTTAA TAGTGTTCAG 
CTTCGACGTA CTGATACTCA AAAAATGCCT AAGCCTTACC AAATGCCTGC ACGCAGTTTA 
AGTATGCAGT GTCTTACCAC GTCAGCTGAT TCTTACCTTA AAACGGATTT AATTGCTGAA 
TTAAAGATTT CAAAAGACAT TACGGGCATT AAGAAAATGA AAGTGGAAAA ACAGTTGGCC 
AGTCAATTTG ACACTGAACA TTATTCAGAA ATTTCCAAAC AATTTTCGAC AAACAACTAT 
ATGGAACAGA TTCCAGAAAA AGATCAGGCA GGTAACATCA TTCCGGATTG GAAACGTCAG 
ATGATGGCGA AAAAGGCAGC CGAACGTGCC AAAAAAGAAT TCGAGGAACG AATGGCAAAA 
GAAGCAGAAA ATAGACGCCT GTCACAAATA CCTCAATGGA AACGTGACCT ACTAGCAAGG 
CGGGAGGAAA CCGAAAATAA ATTAAAAGCA TCAATTTATA CACCCAAGGT GGAGGAAAAT 
AATCGTATAG CTGAAACTTG GCATCTTAAG AATCGAGCAA TTTCAATTGA TAACATTAAT 
TTAGTTTCCC CGAGCGTTGA TTCACAGCTA CTTGCTTCGG GAACTTTTGA CAGTAACAAA 
GAAAATAAGG ATCAAAATGA ACAAGCCCTA ATAAGTAACA TTCTAACAAC GGATGAAGAC 
ATCAATAGTA ATCAATATAG TAAAGGAGAT GACGCCGATA ATATAATACC ATGGCGGGCC 
CAGTTACGAA AAACTAATAG TAGACTAAGT TTAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	Espn
Entrez Gene ID	105212834
Full Name	Espin
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

Espn cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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