LOC105212708 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105212708
Entrez Gene ID	105212708
Full Name	ATP-binding cassette sub-family F member 1
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011184875.2	XP_011183177.1	ATP-binding cassette sub-family F member 1
XM_011184875.1	XP_011183177.1	ATP-binding cassette sub-family F member 1

Pathways from BioSystems
Gene Ontology

The following LOC105212708 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105212708 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa198971	XM_011184875.2 Latest version!	Zeugodacus cucurbitae ATP-binding cassette sub-family F member 1 (LOC105212708), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OBa198971	XM_011184875.1	Bactrocera cucurbitae ATP-binding cassette sub-family F member 1 (LOC105212708), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105212708 ( XM_011184875.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183177.1 Zeugodacus cucurbitae ATP-binding cassette sub-family F member 1 (LOC105212708), mRNA.

Price & Availability↑

CloneID	OBa198971
Clone ID Related Accession (Same CDS sequence)	XM_011184875.2 , XM_011184875.1
Accession Version	XM_011184875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	ATP-binding cassette sub-family F member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863708.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011184875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGG CCAAAGGGAA AAAGAAAAAT GAACGGTTTG AGGATGAGGA GGATGCAGAG 
CAAGTGAAGA CAGTGCCTAA CAAGAAGTCT AGTAAGAAAG GAAAAAAGGG TAAACGTGGC 
GGAGATTCAA GTGAAGACGA GTCTTTGGCT GGTAGTAAAG TGACTAGTAT TGACGCTGAT 
GATTTGGAAA GTGTTAAGTC GGGAAGTAAA ACAGCACCAT CAAAAGGAAA AAAGGGAAAG 
AAAGGAAAGC GGAAAGACGA CTGGAGTGAT AATGATGATG AACCAGAGGA CATCGTACAA 
ATTGGTGGCA AGAAGAATGT ATCAAAATCA CAAGATAGTG ATGATGAGGA AGTGAATGTT 
CCTCAACCAA CTTCCATTAC GAAGAAGCAG GGAAAGAAAG GAAAAAAGAA CAAAAAGAAG 
GATGACTTCA GCGATGATGA GGACAGTAGT AATCAAAAGT CGAAATTCCT TGATTTGGAA 
GATGATGAAG AAATTGACGA TGAAGAACTT CCTCCTGCAG TCGCCAAATC ATCAGCAAAG 
AAATCTGCAA AAAAACAACA AAAAGCCAAT AAATATGCTA TTAGTGACGA TGAAGATGAA 
AATATTGAGG ATGAGGATGA GGATGATTTA GAGGAAAAAC AAGATGAAAA CCCAATTAAA 
TCAGATAAAA TTGTATCGGA ACTAAGCGTA GAAAATAAAG AAAATATATC AGAAGATTCG 
GAAATAACGA AACAAATTAA TGAAATGCAA ATTGAAGATA ATGTGCCTAA AGAAGCGGAA 
CCTTTACTCG ATGAAGGTCA AACACAAGAA GAAGAAATCA AAGAAACAGC TAAAGAGGAT 
ACAATTGAAT CCACTTTAAA AGAAAAAAAG CTCACTCACA AGGAAAAGAA GAAGCAAAAG 
AAGCAGCAGG AATACGAAAA ACAAATGGAG CTTATGACAA AGAAAGGTGG CTCAGGACAT 
TCCGACTTGG ATAGCAATTT TACTATGTCT CAAGTGCAAC GTACTGCTGG TCAGCAAGCT 
CAATTAGAGC ATGCCGTTGA TATCAAAATC GAAAATTTTA CTATATCGGC AAAAGGCAAT 
GATTTATTTG TAAATGCTAA CTTGTTGATT GCTCACGGTC GTCGCTACGG TTTGGTAGGT 
CCCAATGGTC ATGGAAAAAC TACTCTACTG CGTCACATAG CCACTCGAGC CTTTGCTATT 
CCACCTAATA TTGATGTATT GCTTTGTGAG CAGGAGGTTG TGGCCACAGA CAAAACTGCG 
ATTGCAACTA TTTTAGAAGC TGACGAAAAG AGAAATCAAC TTCTGGCAAA GAGCAAAGAG 
TTAGAGGATC AATTTGCTGC TGGCGATATG AGTGTGCAGG AGGAACTAAA TGACACGTTT 
GCAGAGTTGA AAGCTATTGG CGCATACTCG GCCGAAGCAC GTGCTCGACG TATATTGGCT 
GGTTTGGGTT TTAGTGAGGA AATGCAAAAC CGTCCGACCA ATAAATTTTC TGGTGGTTGG 
CGTATGCGAG TCTCATTAGC TCGTGCCCTT TATTTAGAAC CAACACTACT TATGCTTGAT 
GAACCTACAA ATCATTTAGA TTTAAATGCT GTCATATGGT TGGACAACTA TTTGCAAGGT 
TGGAAGAAGA CTCTATTGAT AGTTTCCCAT GACCAGAGTT TCTTGGATAA CGTATGTAAC 
GAAATAATCC ATCTTGATCA AAAGAAGCTT CATTATTACA AAGGTAATTA CTCCATGTTT 
AAGAAAATGT ATGTTCAGAA ACGTCGTGAA ATGATTAAAG AATATGAGAA GCAAGAGAAA 
CGTTTGAAGG AGTTGAAAGC TCATGGTCAA TCGAAAAAGG CGGCTGAGAA AAAACAAAAA 
GAAGTCCTCA CGCGCAAACA AGAAAAAAAT AAGTCTAAGC AACAAAAACA GGACGATGAT 
GACGGCCCAC AGGAATTATT GGCTCGTCCA AAGGAGTATA TAGTAAAATT CCGTTTTCCA 
GAGCCATCAC AGTTACAGCC ACCTATTCTA GGAATCCACA ACGTTACATT TGAATATGAA 
GGCCAGAAAC CTCTGTTTGT CAATACCGAC TTCGGTATAG ACCTCACCAG TCGTGTAGCC 
ATTGTTGGGC CGAATGGCGT GGGCAAATCT ACTTTCCTAA AATTGTTGTT AGGTGAACTT 
GAGCCACAAA AAGGGGAACA AAGAAAAAAT CATAGACTAC GTGTTGGACG CTTTGATCAA 
CACTCCGGTG AACATTTGAC AGCTGAGGAA TCCGCGGCGG AGTATCTGCA GCGTCTGTTT 
AATCTGCAAC ATGAGAAAGC ACGTAAAGCA CTTGGCTCCT TCGGACTGGT AAGCCATGCT 
CATACAATTA AAATGAAGGA TCTTTCTGGT GGTCAAAAAG CAAGAGTTGC ACTTGCAGAG 
CTTTGTCTAA GTGCGCCCGA TGTTTTGATT CTTGACGAAC CTACTAATAA TTTGGATATT 
GAGTCTATTG ATGCATTGGC AGAGGCCATC AACGAATATG AGGGTGGAGT AATAATAGTG 
TCTCACGACG AAAGATTGAT ACGTGAAACT GGATGTACAC TGTATGTAAT TGAGGATCAG 
ACGATCAATG AAATCGATGG GGAATTCGAC GACTACCGCA AGGAAGTGCT CGACGCACTC 
GGCGAGGTTG TCAATAATCC CAGTGTCGTT GCAAATGCAG CTGTATCACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183177.1
CDS	140..2833
Translation	MSKAKGKKKNERFEDEEDAEQVKTVPNKKSSKKGKKGKRGGDSSEDESLAGSKVTSIDADDLESVKSGSKTAPSKGKKGKKGKRKDDWSDNDDEPEDIVQIGGKKNVSKSQDSDDEEVNVPQPTSITKKQGKKGKKNKKKDDFSDDEDSSNQKSKFLDLEDDEEIDDEELPPAVAKSSAKKSAKKQQKANKYAISDDEDENIEDEDEDDLEEKQDENPIKSDKIVSELSVENKENISEDSEITKQINEMQIEDNVPKEAEPLLDEGQTQEEEIKETAKEDTIESTLKEKKLTHKEKKKQKKQQEYEKQMELMTKKGGSGHSDLDSNFTMSQVQRTAGQQAQLEHAVDIKIENFTISAKGNDLFVNANLLIAHGRRYGLVGPNGHGKTTLLRHIATRAFAIPPNIDVLLCEQEVVATDKTAIATILEADEKRNQLLAKSKELEDQFAAGDMSVQEELNDTFAELKAIGAYSAEARARRILAGLGFSEEMQNRPTNKFSGGWRMRVSLARALYLEPTLLMLDEPTNHLDLNAVIWLDNYLQGWKKTLLIVSHDQSFLDNVCNEIIHLDQKKLHYYKGNYSMFKKMYVQKRREMIKEYEKQEKRLKELKAHGQSKKAAEKKQKEVLTRKQEKNKSKQQKQDDDDGPQELLARPKEYIVKFRFPEPSQLQPPILGIHNVTFEYEGQKPLFVNTDFGIDLTSRVAIVGPNGVGKSTFLKLLLGELEPQKGEQRKNHRLRVGRFDQHSGEHLTAEESAAEYLQRLFNLQHEKARKALGSFGLVSHAHTIKMKDLSGGQKARVALAELCLSAPDVLILDEPTNNLDIESIDALAEAINEYEGGVIIVSHDERLIRETGCTLYVIEDQTINEIDGEFDDYRKEVLDALGEVVNNPSVVANAAVSQ

Target ORF information:

RefSeq Version	XM_011184875.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae ATP-binding cassette sub-family F member 1 (LOC105212708), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011184875.2

ORF Insert Sequence:

ATGTCAAAGG CCAAAGGGAA AAAGAAAAAT GAACGGTTTG AGGATGAGGA GGATGCAGAG 
CAAGTGAAGA CAGTGCCTAA CAAGAAGTCT AGTAAGAAAG GAAAAAAGGG TAAACGTGGC 
GGAGATTCAA GTGAAGACGA GTCTTTGGCT GGTAGTAAAG TGACTAGTAT TGACGCTGAT 
GATTTGGAAA GTGTTAAGTC GGGAAGTAAA ACAGCACCAT CAAAAGGAAA AAAGGGAAAG 
AAAGGAAAGC GGAAAGACGA CTGGAGTGAT AATGATGATG AACCAGAGGA CATCGTACAA 
ATTGGTGGCA AGAAGAATGT ATCAAAATCA CAAGATAGTG ATGATGAGGA AGTGAATGTT 
CCTCAACCAA CTTCCATTAC GAAGAAGCAG GGAAAGAAAG GAAAAAAGAA CAAAAAGAAG 
GATGACTTCA GCGATGATGA GGACAGTAGT AATCAAAAGT CGAAATTCCT TGATTTGGAA 
GATGATGAAG AAATTGACGA TGAAGAACTT CCTCCTGCAG TCGCCAAATC ATCAGCAAAG 
AAATCTGCAA AAAAACAACA AAAAGCCAAT AAATATGCTA TTAGTGACGA TGAAGATGAA 
AATATTGAGG ATGAGGATGA GGATGATTTA GAGGAAAAAC AAGATGAAAA CCCAATTAAA 
TCAGATAAAA TTGTATCGGA ACTAAGCGTA GAAAATAAAG AAAATATATC AGAAGATTCG 
GAAATAACGA AACAAATTAA TGAAATGCAA ATTGAAGATA ATGTGCCTAA AGAAGCGGAA 
CCTTTACTCG ATGAAGGTCA AACACAAGAA GAAGAAATCA AAGAAACAGC TAAAGAGGAT 
ACAATTGAAT CCACTTTAAA AGAAAAAAAG CTCACTCACA AGGAAAAGAA GAAGCAAAAG 
AAGCAGCAGG AATACGAAAA ACAAATGGAG CTTATGACAA AGAAAGGTGG CTCAGGACAT 
TCCGACTTGG ATAGCAATTT TACTATGTCT CAAGTGCAAC GTACTGCTGG TCAGCAAGCT 
CAATTAGAGC ATGCCGTTGA TATCAAAATC GAAAATTTTA CTATATCGGC AAAAGGCAAT 
GATTTATTTG TAAATGCTAA CTTGTTGATT GCTCACGGTC GTCGCTACGG TTTGGTAGGT 
CCCAATGGTC ATGGAAAAAC TACTCTACTG CGTCACATAG CCACTCGAGC CTTTGCTATT 
CCACCTAATA TTGATGTATT GCTTTGTGAG CAGGAGGTTG TGGCCACAGA CAAAACTGCG 
ATTGCAACTA TTTTAGAAGC TGACGAAAAG AGAAATCAAC TTCTGGCAAA GAGCAAAGAG 
TTAGAGGATC AATTTGCTGC TGGCGATATG AGTGTGCAGG AGGAACTAAA TGACACGTTT 
GCAGAGTTGA AAGCTATTGG CGCATACTCG GCCGAAGCAC GTGCTCGACG TATATTGGCT 
GGTTTGGGTT TTAGTGAGGA AATGCAAAAC CGTCCGACCA ATAAATTTTC TGGTGGTTGG 
CGTATGCGAG TCTCATTAGC TCGTGCCCTT TATTTAGAAC CAACACTACT TATGCTTGAT 
GAACCTACAA ATCATTTAGA TTTAAATGCT GTCATATGGT TGGACAACTA TTTGCAAGGT 
TGGAAGAAGA CTCTATTGAT AGTTTCCCAT GACCAGAGTT TCTTGGATAA CGTATGTAAC 
GAAATAATCC ATCTTGATCA AAAGAAGCTT CATTATTACA AAGGTAATTA CTCCATGTTT 
AAGAAAATGT ATGTTCAGAA ACGTCGTGAA ATGATTAAAG AATATGAGAA GCAAGAGAAA 
CGTTTGAAGG AGTTGAAAGC TCATGGTCAA TCGAAAAAGG CGGCTGAGAA AAAACAAAAA 
GAAGTCCTCA CGCGCAAACA AGAAAAAAAT AAGTCTAAGC AACAAAAACA GGACGATGAT 
GACGGCCCAC AGGAATTATT GGCTCGTCCA AAGGAGTATA TAGTAAAATT CCGTTTTCCA 
GAGCCATCAC AGTTACAGCC ACCTATTCTA GGAATCCACA ACGTTACATT TGAATATGAA 
GGCCAGAAAC CTCTGTTTGT CAATACCGAC TTCGGTATAG ACCTCACCAG TCGTGTAGCC 
ATTGTTGGGC CGAATGGCGT GGGCAAATCT ACTTTCCTAA AATTGTTGTT AGGTGAACTT 
GAGCCACAAA AAGGGGAACA AAGAAAAAAT CATAGACTAC GTGTTGGACG CTTTGATCAA 
CACTCCGGTG AACATTTGAC AGCTGAGGAA TCCGCGGCGG AGTATCTGCA GCGTCTGTTT 
AATCTGCAAC ATGAGAAAGC ACGTAAAGCA CTTGGCTCCT TCGGACTGGT AAGCCATGCT 
CATACAATTA AAATGAAGGA TCTTTCTGGT GGTCAAAAAG CAAGAGTTGC ACTTGCAGAG 
CTTTGTCTAA GTGCGCCCGA TGTTTTGATT CTTGACGAAC CTACTAATAA TTTGGATATT 
GAGTCTATTG ATGCATTGGC AGAGGCCATC AACGAATATG AGGGTGGAGT AATAATAGTG 
TCTCACGACG AAAGATTGAT ACGTGAAACT GGATGTACAC TGTATGTAAT TGAGGATCAG 
ACGATCAATG AAATCGATGG GGAATTCGAC GACTACCGCA AGGAAGTGCT CGACGCACTC 
GGCGAGGTTG TCAATAATCC CAGTGTCGTT GCAAATGCAG CTGTATCACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105212708 ( XM_011184875.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183177.1 Bactrocera cucurbitae ATP-binding cassette sub-family F member 1 (LOC105212708), mRNA.

Price & Availability↑

CloneID	OBa198971
Clone ID Related Accession (Same CDS sequence)	XM_011184875.2 , XM_011184875.1
Accession Version	XM_011184875.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	ATP-binding cassette sub-family F member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863708.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGG CCAAAGGGAA AAAGAAAAAT GAACGGTTTG AGGATGAGGA GGATGCAGAG 
CAAGTGAAGA CAGTGCCTAA CAAGAAGTCT AGTAAGAAAG GAAAAAAGGG TAAACGTGGC 
GGAGATTCAA GTGAAGACGA GTCTTTGGCT GGTAGTAAAG TGACTAGTAT TGACGCTGAT 
GATTTGGAAA GTGTTAAGTC GGGAAGTAAA ACAGCACCAT CAAAAGGAAA AAAGGGAAAG 
AAAGGAAAGC GGAAAGACGA CTGGAGTGAT AATGATGATG AACCAGAGGA CATCGTACAA 
ATTGGTGGCA AGAAGAATGT ATCAAAATCA CAAGATAGTG ATGATGAGGA AGTGAATGTT 
CCTCAACCAA CTTCCATTAC GAAGAAGCAG GGAAAGAAAG GAAAAAAGAA CAAAAAGAAG 
GATGACTTCA GCGATGATGA GGACAGTAGT AATCAAAAGT CGAAATTCCT TGATTTGGAA 
GATGATGAAG AAATTGACGA TGAAGAACTT CCTCCTGCAG TCGCCAAATC ATCAGCAAAG 
AAATCTGCAA AAAAACAACA AAAAGCCAAT AAATATGCTA TTAGTGACGA TGAAGATGAA 
AATATTGAGG ATGAGGATGA GGATGATTTA GAGGAAAAAC AAGATGAAAA CCCAATTAAA 
TCAGATAAAA TTGTATCGGA ACTAAGCGTA GAAAATAAAG AAAATATATC AGAAGATTCG 
GAAATAACGA AACAAATTAA TGAAATGCAA ATTGAAGATA ATGTGCCTAA AGAAGCGGAA 
CCTTTACTCG ATGAAGGTCA AACACAAGAA GAAGAAATCA AAGAAACAGC TAAAGAGGAT 
ACAATTGAAT CCACTTTAAA AGAAAAAAAG CTCACTCACA AGGAAAAGAA GAAGCAAAAG 
AAGCAGCAGG AATACGAAAA ACAAATGGAG CTTATGACAA AGAAAGGTGG CTCAGGACAT 
TCCGACTTGG ATAGCAATTT TACTATGTCT CAAGTGCAAC GTACTGCTGG TCAGCAAGCT 
CAATTAGAGC ATGCCGTTGA TATCAAAATC GAAAATTTTA CTATATCGGC AAAAGGCAAT 
GATTTATTTG TAAATGCTAA CTTGTTGATT GCTCACGGTC GTCGCTACGG TTTGGTAGGT 
CCCAATGGTC ATGGAAAAAC TACTCTACTG CGTCACATAG CCACTCGAGC CTTTGCTATT 
CCACCTAATA TTGATGTATT GCTTTGTGAG CAGGAGGTTG TGGCCACAGA CAAAACTGCG 
ATTGCAACTA TTTTAGAAGC TGACGAAAAG AGAAATCAAC TTCTGGCAAA GAGCAAAGAG 
TTAGAGGATC AATTTGCTGC TGGCGATATG AGTGTGCAGG AGGAACTAAA TGACACGTTT 
GCAGAGTTGA AAGCTATTGG CGCATACTCG GCCGAAGCAC GTGCTCGACG TATATTGGCT 
GGTTTGGGTT TTAGTGAGGA AATGCAAAAC CGTCCGACCA ATAAATTTTC TGGTGGTTGG 
CGTATGCGAG TCTCATTAGC TCGTGCCCTT TATTTAGAAC CAACACTACT TATGCTTGAT 
GAACCTACAA ATCATTTAGA TTTAAATGCT GTCATATGGT TGGACAACTA TTTGCAAGGT 
TGGAAGAAGA CTCTATTGAT AGTTTCCCAT GACCAGAGTT TCTTGGATAA CGTATGTAAC 
GAAATAATCC ATCTTGATCA AAAGAAGCTT CATTATTACA AAGGTAATTA CTCCATGTTT 
AAGAAAATGT ATGTTCAGAA ACGTCGTGAA ATGATTAAAG AATATGAGAA GCAAGAGAAA 
CGTTTGAAGG AGTTGAAAGC TCATGGTCAA TCGAAAAAGG CGGCTGAGAA AAAACAAAAA 
GAAGTCCTCA CGCGCAAACA AGAAAAAAAT AAGTCTAAGC AACAAAAACA GGACGATGAT 
GACGGCCCAC AGGAATTATT GGCTCGTCCA AAGGAGTATA TAGTAAAATT CCGTTTTCCA 
GAGCCATCAC AGTTACAGCC ACCTATTCTA GGAATCCACA ACGTTACATT TGAATATGAA 
GGCCAGAAAC CTCTGTTTGT CAATACCGAC TTCGGTATAG ACCTCACCAG TCGTGTAGCC 
ATTGTTGGGC CGAATGGCGT GGGCAAATCT ACTTTCCTAA AATTGTTGTT AGGTGAACTT 
GAGCCACAAA AAGGGGAACA AAGAAAAAAT CATAGACTAC GTGTTGGACG CTTTGATCAA 
CACTCCGGTG AACATTTGAC AGCTGAGGAA TCCGCGGCGG AGTATCTGCA GCGTCTGTTT 
AATCTGCAAC ATGAGAAAGC ACGTAAAGCA CTTGGCTCCT TCGGACTGGT AAGCCATGCT 
CATACAATTA AAATGAAGGA TCTTTCTGGT GGTCAAAAAG CAAGAGTTGC ACTTGCAGAG 
CTTTGTCTAA GTGCGCCCGA TGTTTTGATT CTTGACGAAC CTACTAATAA TTTGGATATT 
GAGTCTATTG ATGCATTGGC AGAGGCCATC AACGAATATG AGGGTGGAGT AATAATAGTG 
TCTCACGACG AAAGATTGAT ACGTGAAACT GGATGTACAC TGTATGTAAT TGAGGATCAG 
ACGATCAATG AAATCGATGG GGAATTCGAC GACTACCGCA AGGAAGTGCT CGACGCACTC 
GGCGAGGTTG TCAATAATCC CAGTGTCGTT GCAAATGCAG CTGTATCACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183177.1
CDS	152..2845
Translation	MSKAKGKKKNERFEDEEDAEQVKTVPNKKSSKKGKKGKRGGDSSEDESLAGSKVTSIDADDLESVKSGSKTAPSKGKKGKKGKRKDDWSDNDDEPEDIVQIGGKKNVSKSQDSDDEEVNVPQPTSITKKQGKKGKKNKKKDDFSDDEDSSNQKSKFLDLEDDEEIDDEELPPAVAKSSAKKSAKKQQKANKYAISDDEDENIEDEDEDDLEEKQDENPIKSDKIVSELSVENKENISEDSEITKQINEMQIEDNVPKEAEPLLDEGQTQEEEIKETAKEDTIESTLKEKKLTHKEKKKQKKQQEYEKQMELMTKKGGSGHSDLDSNFTMSQVQRTAGQQAQLEHAVDIKIENFTISAKGNDLFVNANLLIAHGRRYGLVGPNGHGKTTLLRHIATRAFAIPPNIDVLLCEQEVVATDKTAIATILEADEKRNQLLAKSKELEDQFAAGDMSVQEELNDTFAELKAIGAYSAEARARRILAGLGFSEEMQNRPTNKFSGGWRMRVSLARALYLEPTLLMLDEPTNHLDLNAVIWLDNYLQGWKKTLLIVSHDQSFLDNVCNEIIHLDQKKLHYYKGNYSMFKKMYVQKRREMIKEYEKQEKRLKELKAHGQSKKAAEKKQKEVLTRKQEKNKSKQQKQDDDDGPQELLARPKEYIVKFRFPEPSQLQPPILGIHNVTFEYEGQKPLFVNTDFGIDLTSRVAIVGPNGVGKSTFLKLLLGELEPQKGEQRKNHRLRVGRFDQHSGEHLTAEESAAEYLQRLFNLQHEKARKALGSFGLVSHAHTIKMKDLSGGQKARVALAELCLSAPDVLILDEPTNNLDIESIDALAEAINEYEGGVIIVSHDERLIRETGCTLYVIEDQTINEIDGEFDDYRKEVLDALGEVVNNPSVVANAAVSQ

Target ORF information:

RefSeq Version	XM_011184875.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae ATP-binding cassette sub-family F member 1 (LOC105212708), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011184875.1

ORF Insert Sequence:

ATGTCAAAGG CCAAAGGGAA AAAGAAAAAT GAACGGTTTG AGGATGAGGA GGATGCAGAG 
CAAGTGAAGA CAGTGCCTAA CAAGAAGTCT AGTAAGAAAG GAAAAAAGGG TAAACGTGGC 
GGAGATTCAA GTGAAGACGA GTCTTTGGCT GGTAGTAAAG TGACTAGTAT TGACGCTGAT 
GATTTGGAAA GTGTTAAGTC GGGAAGTAAA ACAGCACCAT CAAAAGGAAA AAAGGGAAAG 
AAAGGAAAGC GGAAAGACGA CTGGAGTGAT AATGATGATG AACCAGAGGA CATCGTACAA 
ATTGGTGGCA AGAAGAATGT ATCAAAATCA CAAGATAGTG ATGATGAGGA AGTGAATGTT 
CCTCAACCAA CTTCCATTAC GAAGAAGCAG GGAAAGAAAG GAAAAAAGAA CAAAAAGAAG 
GATGACTTCA GCGATGATGA GGACAGTAGT AATCAAAAGT CGAAATTCCT TGATTTGGAA 
GATGATGAAG AAATTGACGA TGAAGAACTT CCTCCTGCAG TCGCCAAATC ATCAGCAAAG 
AAATCTGCAA AAAAACAACA AAAAGCCAAT AAATATGCTA TTAGTGACGA TGAAGATGAA 
AATATTGAGG ATGAGGATGA GGATGATTTA GAGGAAAAAC AAGATGAAAA CCCAATTAAA 
TCAGATAAAA TTGTATCGGA ACTAAGCGTA GAAAATAAAG AAAATATATC AGAAGATTCG 
GAAATAACGA AACAAATTAA TGAAATGCAA ATTGAAGATA ATGTGCCTAA AGAAGCGGAA 
CCTTTACTCG ATGAAGGTCA AACACAAGAA GAAGAAATCA AAGAAACAGC TAAAGAGGAT 
ACAATTGAAT CCACTTTAAA AGAAAAAAAG CTCACTCACA AGGAAAAGAA GAAGCAAAAG 
AAGCAGCAGG AATACGAAAA ACAAATGGAG CTTATGACAA AGAAAGGTGG CTCAGGACAT 
TCCGACTTGG ATAGCAATTT TACTATGTCT CAAGTGCAAC GTACTGCTGG TCAGCAAGCT 
CAATTAGAGC ATGCCGTTGA TATCAAAATC GAAAATTTTA CTATATCGGC AAAAGGCAAT 
GATTTATTTG TAAATGCTAA CTTGTTGATT GCTCACGGTC GTCGCTACGG TTTGGTAGGT 
CCCAATGGTC ATGGAAAAAC TACTCTACTG CGTCACATAG CCACTCGAGC CTTTGCTATT 
CCACCTAATA TTGATGTATT GCTTTGTGAG CAGGAGGTTG TGGCCACAGA CAAAACTGCG 
ATTGCAACTA TTTTAGAAGC TGACGAAAAG AGAAATCAAC TTCTGGCAAA GAGCAAAGAG 
TTAGAGGATC AATTTGCTGC TGGCGATATG AGTGTGCAGG AGGAACTAAA TGACACGTTT 
GCAGAGTTGA AAGCTATTGG CGCATACTCG GCCGAAGCAC GTGCTCGACG TATATTGGCT 
GGTTTGGGTT TTAGTGAGGA AATGCAAAAC CGTCCGACCA ATAAATTTTC TGGTGGTTGG 
CGTATGCGAG TCTCATTAGC TCGTGCCCTT TATTTAGAAC CAACACTACT TATGCTTGAT 
GAACCTACAA ATCATTTAGA TTTAAATGCT GTCATATGGT TGGACAACTA TTTGCAAGGT 
TGGAAGAAGA CTCTATTGAT AGTTTCCCAT GACCAGAGTT TCTTGGATAA CGTATGTAAC 
GAAATAATCC ATCTTGATCA AAAGAAGCTT CATTATTACA AAGGTAATTA CTCCATGTTT 
AAGAAAATGT ATGTTCAGAA ACGTCGTGAA ATGATTAAAG AATATGAGAA GCAAGAGAAA 
CGTTTGAAGG AGTTGAAAGC TCATGGTCAA TCGAAAAAGG CGGCTGAGAA AAAACAAAAA 
GAAGTCCTCA CGCGCAAACA AGAAAAAAAT AAGTCTAAGC AACAAAAACA GGACGATGAT 
GACGGCCCAC AGGAATTATT GGCTCGTCCA AAGGAGTATA TAGTAAAATT CCGTTTTCCA 
GAGCCATCAC AGTTACAGCC ACCTATTCTA GGAATCCACA ACGTTACATT TGAATATGAA 
GGCCAGAAAC CTCTGTTTGT CAATACCGAC TTCGGTATAG ACCTCACCAG TCGTGTAGCC 
ATTGTTGGGC CGAATGGCGT GGGCAAATCT ACTTTCCTAA AATTGTTGTT AGGTGAACTT 
GAGCCACAAA AAGGGGAACA AAGAAAAAAT CATAGACTAC GTGTTGGACG CTTTGATCAA 
CACTCCGGTG AACATTTGAC AGCTGAGGAA TCCGCGGCGG AGTATCTGCA GCGTCTGTTT 
AATCTGCAAC ATGAGAAAGC ACGTAAAGCA CTTGGCTCCT TCGGACTGGT AAGCCATGCT 
CATACAATTA AAATGAAGGA TCTTTCTGGT GGTCAAAAAG CAAGAGTTGC ACTTGCAGAG 
CTTTGTCTAA GTGCGCCCGA TGTTTTGATT CTTGACGAAC CTACTAATAA TTTGGATATT 
GAGTCTATTG ATGCATTGGC AGAGGCCATC AACGAATATG AGGGTGGAGT AATAATAGTG 
TCTCACGACG AAAGATTGAT ACGTGAAACT GGATGTACAC TGTATGTAAT TGAGGATCAG 
ACGATCAATG AAATCGATGG GGAATTCGAC GACTACCGCA AGGAAGTGCT CGACGCACTC 
GGCGAGGTTG TCAATAATCC CAGTGTCGTT GCAAATGCAG CTGTATCACA ATAA