LOC105212649 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105212649
Entrez Gene ID	105212649
Full Name	ubiquitin carboxyl-terminal hydrolase 36
Synonyms	Usp36
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011184778.1	XP_011183080.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X2
XM_011184769.1	XP_011183071.1	ubiquitin carboxyl-terminal hydrolase 36 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105212649 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105212649 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa191801	XM_011184769.1 Latest version!	Zeugodacus cucurbitae ubiquitin carboxyl-terminal hydrolase 36 (LOC105212649), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa191802	XM_011184778.1 Latest version!	Zeugodacus cucurbitae ubiquitin carboxyl-terminal hydrolase 36 (LOC105212649), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105212649 ( XM_011184769.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183071.1 Zeugodacus cucurbitae ubiquitin carboxyl-terminal hydrolase 36 (LOC105212649), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa191801
Clone ID Related Accession (Same CDS sequence)	XM_011184769.1
Accession Version	XM_011184769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGT CAGTGTGTGA ACCAGTGGCT AACGTTAGCG CAGTTTTGCG GGAATCACTG 
GGAAATGGGT ATTCAAGCAA TGGTAGAAAC ATTTGTAAAG CAGGAGGTGT GGGTGATAAC 
TACAATGCCG ATGATGATGG ATGTCTACAG GCAAAACTCG CTGTCACAGC AAAACGTGTG 
TTACTATCGA AAATAGAATA TGAGGAGGTA ACAAACTACG GTCAATCTGT TTTAGGTGCA 
CTAAAATCAA AATATGATAT TTTAAAAGCC AATACAACTA ATAAAAATTC AAACTATGAT 
TACCTCTCTC TTGGGAAGAA TTCGGAACAA AATGACACAT CTACTATTGC AGCGCCGTCA 
AATGGTGCTT CTTCGATTCC AAAGCGACAA AATCCCAACG AATTGCCACA ACCAAAGAGA 
GTGCTGTATC CTCGGGAAAA TGTAATAATT GGTTGGAAAA ATTCAGCACG TAAGTGGCAT 
GTTGGGGTCG GTATGATGAA TGTCGGAAAT ACATGCTACC TAAATTCAAC ATTACAAGCC 
CTCTTTCATG TACCCTCTAT GGCGAACTGG CTAATTTCAG ATGTGAAACA TATAGAAGAA 
TGTAATTCAT CTGAAAACTG TATAATATGT GCAATGATTA AAACCTTAGA GGCATCACAG 
AATAATCAAT CAGCAATTCG CCCATACTTG GTTTATTCGA AACTTAAGTT CATTTGCAAA 
CACTTACTTA TGGGTAGACA AGAGGATGCA CATGAGTTTC TCAGATATTT AGTCGAGGCA 
ATGGAGAAAT GTTTCCTTAC CCGCTTTCGG AACTACAAAG AATTAGATCA GTATAGCAAA 
GAAACAACAC CGCTTAATCA AATCCTCGGA GGTTATTTAA AATCAGCCGT AAGGTGTTTA 
TCTTGTGGAC ATGTGTCTGT AACGTTCCAA CATTTTCAAG ATTTACTATT AGATATAAGA 
AAAGCTGATA CAATTGATGA AGCCTTAGAA GGCTACTTTT CAAGAGAACG TCTTGAAGAT 
ATGGGATATA AATGCGAATC GTGCAAAAAA AAGGTCTCTG CTACCAAGCA ATTTTCTTTA 
GAACGTGCTC CAATTGTGCT TTGCATACAG CTAAAACGAT TTTCAGTTAT GGGTACCAAG 
CTTAACAAAC AAATTACAAT CAAACCACGA ATCGATTTAT CTAAATTTGT TTCTCGAAAG 
GAAGTCGGTG AACAGTTAAC TTATAAATTA GTCGCTATGG TTACCCATTT GGGGGCTTCA 
CAACATTGTG GTCATTACAC TGCCATTGGT TTGACCGAAT CGGGTTCTTA TTATAATTAC 
GATGATAGCT ACGTTCGCCC AGTTTCTGTA CAGAATGTTT GCAATACAAA CGCTTATATT 
ATATTTTATG AAATTGAAAA GCCACAACGT TCTCCAAATA ATTCCGAACT GAGCAGCATA 
AGTGATGTTG TTAAGGAAAA TTCGCAAAAA CAAAATGGTT ATACAAAACC TGAAAATATA 
TTCTCCACTG AAATAAGTAG TTCAAATAAT TCACAACAAC GTCTTGATAA TAATTTATTG 
ACCGGAGGTA TTAAATCATC ACCATCATAT TATATTAATA AGCCGTCCAC AAGTAATGGA 
TCCATATCTA GTAAATTAAC TGTTAGCCGC CAGCTTCAAT TTAAAGCTCA AACTAATAGC 
ACACCGTTGA AATCAAATGA TTCACCATCA ACAAAGTTAT CAATGAATTC CGAAAATAGA 
AATATCATTC AACGGCCTTC GGAGCAAACT AGTATCTTGC AGGGAAAACA CTATGATAAT 
AAAGATGCGG AGAAAAAATC AAACTCACCA ACTACTGATA ATAAGCGTAG TAAAGTTTCA 
GAGCTGGTGT CACTACATAA AGCAGCTGCA ACCCTGCCTA GTATGCCAAA TTTATCTGAT 
GTTCAACATC AAAATTTAAA TCAACCTGCC AAATATTCTG CGGTATCAGT CACCTCTTTA 
AAGAGCCTTG TCCCGTATGA ATCTGAAACA GATGAAGAAT CGACGGCGTC TGAGGTAAAG 
GATATATTGT TAGGTTCGGA AGATATTGAC AAGAAAAAGA AAGATATCAA AAGTGCTATT 
AAACGACCGT TGGAAAGCCC TTCACAAAAT ACCACTGCAA CCACGGAAAG TTTAAAACAA 
AAAAAGTTGG CTATTTTGAG TGAAAATGAT TTAAATACAA ACAAATCAGA CGAAACAAAT 
ACAAATCGTT CAAAGGGTAA ACATAAAGTT GATATATTTG ATGAAATTTT TAATCAAAAG 
AAATGTGTTC GTAGCAAAGA TGATGTAACT TCAAGTGAAG ATTTCACAAA TAAGACGCAC 
GATGAGGACG AATGTGATGA TCATTTAGTT TCATCGAGTG TATCTCGACC ACCGTTTGTA 
CGTTCACATT CTACACCGCC TTCTCCACCT GTCATTAAAA CAAAAACAGG TTTATGGCAA 
GTCACTACAT TACAGCCGGT GACAGCGTGT ATCAGTCCGC CGGAGAAAAA AGTGTTAAAA 
AATGTTAAGA ACCCATTCGC CAAAAAATCA TTGGACACAT TTAATAAACG TGCAAAGCCC 
AACCCGGAGG TGAAGTCTGA AAAGACCTTT GCGGGTAATG GCTATAGATG CAGCAATGTA 
ACCGATGATT CCGTATCAGG TCTATTGAAA CAGTCTCATC GTGGATATGG TGTTCCCGTT 
CTCTCATGGA AGGGAGACCA AACAAAAATA TCTAAAGAGG TGGAAATTGA TGCTCAACAA 
CAGCGACAAA ATGACTGGCA AAATGATGAA GATGCCGAAA TCGACCGTGG ACGTCAGAAA 
AAAGTGAAGA AAGAAGGATC ACAATCCGAT AATCGAATTC CGGGATTCAA CCCATTTCAA 
GAACAAGAAA ATCAAAGACG ATGGAGATCC AACGGGGTAA CTAATAGGCA TTATAGTGGA 
TATGTTCGAC AACAATTCCA ACGCAGCAAA TTCAAGTTTC AACGGTTCAA TCCGAAATTC 
CAGCGAATGA CTGCAATGTC TTATAAGCGA AACAATCACA GATTGAATAG CGGTTATACA 
CGACGGAACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183071.1
CDS	439..3513
Translation	MPVSVCEPVANVSAVLRESLGNGYSSNGRNICKAGGVGDNYNADDDGCLQAKLAVTAKRVLLSKIEYEEVTNYGQSVLGALKSKYDILKANTTNKNSNYDYLSLGKNSEQNDTSTIAAPSNGASSIPKRQNPNELPQPKRVLYPRENVIIGWKNSARKWHVGVGMMNVGNTCYLNSTLQALFHVPSMANWLISDVKHIEECNSSENCIICAMIKTLEASQNNQSAIRPYLVYSKLKFICKHLLMGRQEDAHEFLRYLVEAMEKCFLTRFRNYKELDQYSKETTPLNQILGGYLKSAVRCLSCGHVSVTFQHFQDLLLDIRKADTIDEALEGYFSRERLEDMGYKCESCKKKVSATKQFSLERAPIVLCIQLKRFSVMGTKLNKQITIKPRIDLSKFVSRKEVGEQLTYKLVAMVTHLGASQHCGHYTAIGLTESGSYYNYDDSYVRPVSVQNVCNTNAYIIFYEIEKPQRSPNNSELSSISDVVKENSQKQNGYTKPENIFSTEISSSNNSQQRLDNNLLTGGIKSSPSYYINKPSTSNGSISSKLTVSRQLQFKAQTNSTPLKSNDSPSTKLSMNSENRNIIQRPSEQTSILQGKHYDNKDAEKKSNSPTTDNKRSKVSELVSLHKAAATLPSMPNLSDVQHQNLNQPAKYSAVSVTSLKSLVPYESETDEESTASEVKDILLGSEDIDKKKKDIKSAIKRPLESPSQNTTATTESLKQKKLAILSENDLNTNKSDETNTNRSKGKHKVDIFDEIFNQKKCVRSKDDVTSSEDFTNKTHDEDECDDHLVSSSVSRPPFVRSHSTPPSPPVIKTKTGLWQVTTLQPVTACISPPEKKVLKNVKNPFAKKSLDTFNKRAKPNPEVKSEKTFAGNGYRCSNVTDDSVSGLLKQSHRGYGVPVLSWKGDQTKISKEVEIDAQQQRQNDWQNDEDAEIDRGRQKKVKKEGSQSDNRIPGFNPFQEQENQRRWRSNGVTNRHYSGYVRQQFQRSKFKFQRFNPKFQRMTAMSYKRNNHRLNSGYTRRNS

Target ORF information:

RefSeq Version	XM_011184769.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae ubiquitin carboxyl-terminal hydrolase 36 (LOC105212649), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011184769.1

ORF Insert Sequence:

ATGCCCGTGT CAGTGTGTGA ACCAGTGGCT AACGTTAGCG CAGTTTTGCG GGAATCACTG 
GGAAATGGGT ATTCAAGCAA TGGTAGAAAC ATTTGTAAAG CAGGAGGTGT GGGTGATAAC 
TACAATGCCG ATGATGATGG ATGTCTACAG GCAAAACTCG CTGTCACAGC AAAACGTGTG 
TTACTATCGA AAATAGAATA TGAGGAGGTA ACAAACTACG GTCAATCTGT TTTAGGTGCA 
CTAAAATCAA AATATGATAT TTTAAAAGCC AATACAACTA ATAAAAATTC AAACTATGAT 
TACCTCTCTC TTGGGAAGAA TTCGGAACAA AATGACACAT CTACTATTGC AGCGCCGTCA 
AATGGTGCTT CTTCGATTCC AAAGCGACAA AATCCCAACG AATTGCCACA ACCAAAGAGA 
GTGCTGTATC CTCGGGAAAA TGTAATAATT GGTTGGAAAA ATTCAGCACG TAAGTGGCAT 
GTTGGGGTCG GTATGATGAA TGTCGGAAAT ACATGCTACC TAAATTCAAC ATTACAAGCC 
CTCTTTCATG TACCCTCTAT GGCGAACTGG CTAATTTCAG ATGTGAAACA TATAGAAGAA 
TGTAATTCAT CTGAAAACTG TATAATATGT GCAATGATTA AAACCTTAGA GGCATCACAG 
AATAATCAAT CAGCAATTCG CCCATACTTG GTTTATTCGA AACTTAAGTT CATTTGCAAA 
CACTTACTTA TGGGTAGACA AGAGGATGCA CATGAGTTTC TCAGATATTT AGTCGAGGCA 
ATGGAGAAAT GTTTCCTTAC CCGCTTTCGG AACTACAAAG AATTAGATCA GTATAGCAAA 
GAAACAACAC CGCTTAATCA AATCCTCGGA GGTTATTTAA AATCAGCCGT AAGGTGTTTA 
TCTTGTGGAC ATGTGTCTGT AACGTTCCAA CATTTTCAAG ATTTACTATT AGATATAAGA 
AAAGCTGATA CAATTGATGA AGCCTTAGAA GGCTACTTTT CAAGAGAACG TCTTGAAGAT 
ATGGGATATA AATGCGAATC GTGCAAAAAA AAGGTCTCTG CTACCAAGCA ATTTTCTTTA 
GAACGTGCTC CAATTGTGCT TTGCATACAG CTAAAACGAT TTTCAGTTAT GGGTACCAAG 
CTTAACAAAC AAATTACAAT CAAACCACGA ATCGATTTAT CTAAATTTGT TTCTCGAAAG 
GAAGTCGGTG AACAGTTAAC TTATAAATTA GTCGCTATGG TTACCCATTT GGGGGCTTCA 
CAACATTGTG GTCATTACAC TGCCATTGGT TTGACCGAAT CGGGTTCTTA TTATAATTAC 
GATGATAGCT ACGTTCGCCC AGTTTCTGTA CAGAATGTTT GCAATACAAA CGCTTATATT 
ATATTTTATG AAATTGAAAA GCCACAACGT TCTCCAAATA ATTCCGAACT GAGCAGCATA 
AGTGATGTTG TTAAGGAAAA TTCGCAAAAA CAAAATGGTT ATACAAAACC TGAAAATATA 
TTCTCCACTG AAATAAGTAG TTCAAATAAT TCACAACAAC GTCTTGATAA TAATTTATTG 
ACCGGAGGTA TTAAATCATC ACCATCATAT TATATTAATA AGCCGTCCAC AAGTAATGGA 
TCCATATCTA GTAAATTAAC TGTTAGCCGC CAGCTTCAAT TTAAAGCTCA AACTAATAGC 
ACACCGTTGA AATCAAATGA TTCACCATCA ACAAAGTTAT CAATGAATTC CGAAAATAGA 
AATATCATTC AACGGCCTTC GGAGCAAACT AGTATCTTGC AGGGAAAACA CTATGATAAT 
AAAGATGCGG AGAAAAAATC AAACTCACCA ACTACTGATA ATAAGCGTAG TAAAGTTTCA 
GAGCTGGTGT CACTACATAA AGCAGCTGCA ACCCTGCCTA GTATGCCAAA TTTATCTGAT 
GTTCAACATC AAAATTTAAA TCAACCTGCC AAATATTCTG CGGTATCAGT CACCTCTTTA 
AAGAGCCTTG TCCCGTATGA ATCTGAAACA GATGAAGAAT CGACGGCGTC TGAGGTAAAG 
GATATATTGT TAGGTTCGGA AGATATTGAC AAGAAAAAGA AAGATATCAA AAGTGCTATT 
AAACGACCGT TGGAAAGCCC TTCACAAAAT ACCACTGCAA CCACGGAAAG TTTAAAACAA 
AAAAAGTTGG CTATTTTGAG TGAAAATGAT TTAAATACAA ACAAATCAGA CGAAACAAAT 
ACAAATCGTT CAAAGGGTAA ACATAAAGTT GATATATTTG ATGAAATTTT TAATCAAAAG 
AAATGTGTTC GTAGCAAAGA TGATGTAACT TCAAGTGAAG ATTTCACAAA TAAGACGCAC 
GATGAGGACG AATGTGATGA TCATTTAGTT TCATCGAGTG TATCTCGACC ACCGTTTGTA 
CGTTCACATT CTACACCGCC TTCTCCACCT GTCATTAAAA CAAAAACAGG TTTATGGCAA 
GTCACTACAT TACAGCCGGT GACAGCGTGT ATCAGTCCGC CGGAGAAAAA AGTGTTAAAA 
AATGTTAAGA ACCCATTCGC CAAAAAATCA TTGGACACAT TTAATAAACG TGCAAAGCCC 
AACCCGGAGG TGAAGTCTGA AAAGACCTTT GCGGGTAATG GCTATAGATG CAGCAATGTA 
ACCGATGATT CCGTATCAGG TCTATTGAAA CAGTCTCATC GTGGATATGG TGTTCCCGTT 
CTCTCATGGA AGGGAGACCA AACAAAAATA TCTAAAGAGG TGGAAATTGA TGCTCAACAA 
CAGCGACAAA ATGACTGGCA AAATGATGAA GATGCCGAAA TCGACCGTGG ACGTCAGAAA 
AAAGTGAAGA AAGAAGGATC ACAATCCGAT AATCGAATTC CGGGATTCAA CCCATTTCAA 
GAACAAGAAA ATCAAAGACG ATGGAGATCC AACGGGGTAA CTAATAGGCA TTATAGTGGA 
TATGTTCGAC AACAATTCCA ACGCAGCAAA TTCAAGTTTC AACGGTTCAA TCCGAAATTC 
CAGCGAATGA CTGCAATGTC TTATAAGCGA AACAATCACA GATTGAATAG CGGTTATACA 
CGACGGAACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105212649 ( XM_011184778.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011183080.1 Zeugodacus cucurbitae ubiquitin carboxyl-terminal hydrolase 36 (LOC105212649), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa191802
Clone ID Related Accession (Same CDS sequence)	XM_011184778.1
Accession Version	XM_011184778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	ubiquitin carboxyl-terminal hydrolase 36 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTGT CAGTGTGTGA ACCAGTGGCT AACGTTAGCG CAGTTTTGCG GGAATCACTG 
GGAAATGGGT ATTCAAGCAA TGGTAGAAAC ATTTGTAAAG CAGGAGGTGT GGGTGATAAC 
TACAATGCCG ATGATGATGG ATGTCTACAG GCAAAACTCG CTGTCACAGC AAAACGTGTG 
TTACTATCGA AAATAGAATA TGAGGAGGTA ACAAACTACG GTCAATCTGT TTTAGGTGCA 
CTAAAATCAA AATATGATAT TTTAAAAGCC AATACAACTA ATAAAAATTC AAACTATGAT 
TACCTCTCTC TTGGGAAGAA TTCGGAACAA AATGACACAT CTACTATTGC AGCGCCGTCA 
AATGGTGCTT CTTCGATTCC AAAGCGACAA AATCCCAACG AATTGCCACA ACCAAAGAGA 
GTGCTGTATC CTCGGGAAAA TGTAATAATT GGTTGGAAAA ATTCAGCACG TAAGTGGCAT 
GTTGGGGTCG GTATGATGAA TGTCGGAAAT ACATGCTACC TAAATTCAAC ATTACAAGCC 
CTCTTTCATG TACCCTCTAT GGCGAACTGG CTAATTTCAG ATGTGAAACA TATAGAAGAA 
TGTAATTCAT CTGAAAACTG TATAATATGT GCAATGATTA AAACCTTAGA GGCATCACAG 
AATAATCAAT CAGCAATTCG CCCATACTTG GTTTATTCGA AACTTAAGTT CATTTGCAAA 
CACTTACTTA TGGGTAGACA AGAGGATGCA CATGAGTTTC TCAGATATTT AGTCGAGGCA 
ATGGAGAAAT GTTTCCTTAC CCGCTTTCGG AACTACAAAG AATTAGATCA GTATAGCAAA 
GAAACAACAC CGCTTAATCA AATCCTCGGA GGTTATTTAA AATCAGCCGT AAGGTGTTTA 
TCTTGTGGAC ATGTGTCTGT AACGTTCCAA CATTTTCAAG ATTTACTATT AGATATAAGA 
AAAGCTGATA CAATTGATGA AGCCTTAGAA GGCTACTTTT CAAGAGAACG TCTTGAAGAT 
ATGGGATATA AATGCGAATC GTGCAAAAAA AAGGTCTCTG CTACCAAGCA ATTTTCTTTA 
GAACGTGCTC CAATTGTGCT TTGCATACAG CTAAAACGAT TTTCAGTTAT GGGTACCAAG 
CTTAACAAAC AAATTACAAT CAAACCACGA ATCGATTTAT CTAAATTTGT TTCTCGAAAG 
GAAGTCGGTG AACAGTTAAC TTATAAATTA GTCGCTATGG TTACCCATTT GGGGGCTTCA 
CAACATTGTG GTCATTACAC TGCCATTGGT TTGACCGAAT CGGGTTCTTA TTATAATTAC 
GATGATAGCT ACGTTCGCCC AGTTTCTGTA CAGAATGTTT GCAATACAAA CGCTTATATT 
ATATTTTATG AAATTGAAAA GCCACAACGT TCTCCAAATA ATTCCGAACT GAGCAGCATA 
AGTGATGTTG TTAAGGAAAA TTCGCAAAAA CAAAATGGTT ATACAAAACC TGAAAATATA 
TTCTCCACTG AAATAAGTAG TTCAAATAAT TCACAACAAC GTCTTGATAA TAATTTATTG 
ACCGGAGGTA TTAAATCATC ACCATCATAT TATATTAATA AGCCGTCCAC AAGTAATGGA 
TCCATATCTA GTAAATTAAC TGTTAGCCGC CAGCTTCAAT TTAAAGCTCA AACTAATAGC 
ACACCGTTGA AATCAAATGA TTCACCATCA ACAAAGTTAT CAATGAATTC CGAAAATAGA 
AATATCATTC AACGGCCTTC GGAGCAAACT AGTATCTTGC AGGGAAAACA CTATGATAAT 
AAAGATGCGG AGAAAAAATC AAACTCACCA ACTACTGATA ATAAGCGTAG TAAAGTTTCA 
GAGCTGGTGT CACTACATAA AGCAGCTGCA ACCCTGCCTA GTATGCCAAA TTTATCTGAT 
GTTCAACATC AAAATTTAAA TCAACCTGCC AAATATTCTG CGGTATCAGT CACCTCTTTA 
AAGAGCCTTG TCCCGTATGA ATCTGAAACA GATGAAGAAT CGACGGCGTC TGAGGTAAAG 
GATATATTGT TAGGTTCGGA AGATATTGAC AAGAAAAAGA AAGATATCAA AAGTGCTATT 
AAACGACCGT TGGAAAGCCC TTCACAAAAT ACCACTGCAA CCACGGAAAG TTTAAAACAA 
AAAAAGTTGG CTATTTTGAG TGAAAATGAT TTAAATACAA ACAAATCAGA CGAAACAAAT 
ACAAATCGTT CAAAGGATGA TGTAACTTCA AGTGAAGATT TCACAAATAA GACGCACGAT 
GAGGACGAAT GTGATGATCA TTTAGTTTCA TCGAGTGTAT CTCGACCACC GTTTGTACGT 
TCACATTCTA CACCGCCTTC TCCACCTGTC ATTAAAACAA AAACAGGTTT ATGGCAAGTC 
ACTACATTAC AGCCGGTGAC AGCGTGTATC AGTCCGCCGG AGAAAAAAGT GTTAAAAAAT 
GTTAAGAACC CATTCGCCAA AAAATCATTG GACACATTTA ATAAACGTGC AAAGCCCAAC 
CCGGAGGTGA AGTCTGAAAA GACCTTTGCG GGTAATGGCT ATAGATGCAG CAATGTAACC 
GATGATTCCG TATCAGGTCT ATTGAAACAG TCTCATCGTG GATATGGTGT TCCCGTTCTC 
TCATGGAAGG GAGACCAAAC AAAAATATCT AAAGAGGTGG AAATTGATGC TCAACAACAG 
CGACAAAATG ACTGGCAAAA TGATGAAGAT GCCGAAATCG ACCGTGGACG TCAGAAAAAA 
GTGAAGAAAG AAGGATCACA ATCCGATAAT CGAATTCCGG GATTCAACCC ATTTCAAGAA 
CAAGAAAATC AAAGACGATG GAGATCCAAC GGGGTAACTA ATAGGCATTA TAGTGGATAT 
GTTCGACAAC AATTCCAACG CAGCAAATTC AAGTTTCAAC GGTTCAATCC GAAATTCCAG 
CGAATGACTG CAATGTCTTA TAAGCGAAAC AATCACAGAT TGAATAGCGG TTATACACGA 
CGGAACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011183080.1
CDS	439..3450
Translation	MPVSVCEPVANVSAVLRESLGNGYSSNGRNICKAGGVGDNYNADDDGCLQAKLAVTAKRVLLSKIEYEEVTNYGQSVLGALKSKYDILKANTTNKNSNYDYLSLGKNSEQNDTSTIAAPSNGASSIPKRQNPNELPQPKRVLYPRENVIIGWKNSARKWHVGVGMMNVGNTCYLNSTLQALFHVPSMANWLISDVKHIEECNSSENCIICAMIKTLEASQNNQSAIRPYLVYSKLKFICKHLLMGRQEDAHEFLRYLVEAMEKCFLTRFRNYKELDQYSKETTPLNQILGGYLKSAVRCLSCGHVSVTFQHFQDLLLDIRKADTIDEALEGYFSRERLEDMGYKCESCKKKVSATKQFSLERAPIVLCIQLKRFSVMGTKLNKQITIKPRIDLSKFVSRKEVGEQLTYKLVAMVTHLGASQHCGHYTAIGLTESGSYYNYDDSYVRPVSVQNVCNTNAYIIFYEIEKPQRSPNNSELSSISDVVKENSQKQNGYTKPENIFSTEISSSNNSQQRLDNNLLTGGIKSSPSYYINKPSTSNGSISSKLTVSRQLQFKAQTNSTPLKSNDSPSTKLSMNSENRNIIQRPSEQTSILQGKHYDNKDAEKKSNSPTTDNKRSKVSELVSLHKAAATLPSMPNLSDVQHQNLNQPAKYSAVSVTSLKSLVPYESETDEESTASEVKDILLGSEDIDKKKKDIKSAIKRPLESPSQNTTATTESLKQKKLAILSENDLNTNKSDETNTNRSKDDVTSSEDFTNKTHDEDECDDHLVSSSVSRPPFVRSHSTPPSPPVIKTKTGLWQVTTLQPVTACISPPEKKVLKNVKNPFAKKSLDTFNKRAKPNPEVKSEKTFAGNGYRCSNVTDDSVSGLLKQSHRGYGVPVLSWKGDQTKISKEVEIDAQQQRQNDWQNDEDAEIDRGRQKKVKKEGSQSDNRIPGFNPFQEQENQRRWRSNGVTNRHYSGYVRQQFQRSKFKFQRFNPKFQRMTAMSYKRNNHRLNSGYTRRNS

Target ORF information:

RefSeq Version	XM_011184778.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae ubiquitin carboxyl-terminal hydrolase 36 (LOC105212649), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011184778.1

ORF Insert Sequence:

ATGCCCGTGT CAGTGTGTGA ACCAGTGGCT AACGTTAGCG CAGTTTTGCG GGAATCACTG 
GGAAATGGGT ATTCAAGCAA TGGTAGAAAC ATTTGTAAAG CAGGAGGTGT GGGTGATAAC 
TACAATGCCG ATGATGATGG ATGTCTACAG GCAAAACTCG CTGTCACAGC AAAACGTGTG 
TTACTATCGA AAATAGAATA TGAGGAGGTA ACAAACTACG GTCAATCTGT TTTAGGTGCA 
CTAAAATCAA AATATGATAT TTTAAAAGCC AATACAACTA ATAAAAATTC AAACTATGAT 
TACCTCTCTC TTGGGAAGAA TTCGGAACAA AATGACACAT CTACTATTGC AGCGCCGTCA 
AATGGTGCTT CTTCGATTCC AAAGCGACAA AATCCCAACG AATTGCCACA ACCAAAGAGA 
GTGCTGTATC CTCGGGAAAA TGTAATAATT GGTTGGAAAA ATTCAGCACG TAAGTGGCAT 
GTTGGGGTCG GTATGATGAA TGTCGGAAAT ACATGCTACC TAAATTCAAC ATTACAAGCC 
CTCTTTCATG TACCCTCTAT GGCGAACTGG CTAATTTCAG ATGTGAAACA TATAGAAGAA 
TGTAATTCAT CTGAAAACTG TATAATATGT GCAATGATTA AAACCTTAGA GGCATCACAG 
AATAATCAAT CAGCAATTCG CCCATACTTG GTTTATTCGA AACTTAAGTT CATTTGCAAA 
CACTTACTTA TGGGTAGACA AGAGGATGCA CATGAGTTTC TCAGATATTT AGTCGAGGCA 
ATGGAGAAAT GTTTCCTTAC CCGCTTTCGG AACTACAAAG AATTAGATCA GTATAGCAAA 
GAAACAACAC CGCTTAATCA AATCCTCGGA GGTTATTTAA AATCAGCCGT AAGGTGTTTA 
TCTTGTGGAC ATGTGTCTGT AACGTTCCAA CATTTTCAAG ATTTACTATT AGATATAAGA 
AAAGCTGATA CAATTGATGA AGCCTTAGAA GGCTACTTTT CAAGAGAACG TCTTGAAGAT 
ATGGGATATA AATGCGAATC GTGCAAAAAA AAGGTCTCTG CTACCAAGCA ATTTTCTTTA 
GAACGTGCTC CAATTGTGCT TTGCATACAG CTAAAACGAT TTTCAGTTAT GGGTACCAAG 
CTTAACAAAC AAATTACAAT CAAACCACGA ATCGATTTAT CTAAATTTGT TTCTCGAAAG 
GAAGTCGGTG AACAGTTAAC TTATAAATTA GTCGCTATGG TTACCCATTT GGGGGCTTCA 
CAACATTGTG GTCATTACAC TGCCATTGGT TTGACCGAAT CGGGTTCTTA TTATAATTAC 
GATGATAGCT ACGTTCGCCC AGTTTCTGTA CAGAATGTTT GCAATACAAA CGCTTATATT 
ATATTTTATG AAATTGAAAA GCCACAACGT TCTCCAAATA ATTCCGAACT GAGCAGCATA 
AGTGATGTTG TTAAGGAAAA TTCGCAAAAA CAAAATGGTT ATACAAAACC TGAAAATATA 
TTCTCCACTG AAATAAGTAG TTCAAATAAT TCACAACAAC GTCTTGATAA TAATTTATTG 
ACCGGAGGTA TTAAATCATC ACCATCATAT TATATTAATA AGCCGTCCAC AAGTAATGGA 
TCCATATCTA GTAAATTAAC TGTTAGCCGC CAGCTTCAAT TTAAAGCTCA AACTAATAGC 
ACACCGTTGA AATCAAATGA TTCACCATCA ACAAAGTTAT CAATGAATTC CGAAAATAGA 
AATATCATTC AACGGCCTTC GGAGCAAACT AGTATCTTGC AGGGAAAACA CTATGATAAT 
AAAGATGCGG AGAAAAAATC AAACTCACCA ACTACTGATA ATAAGCGTAG TAAAGTTTCA 
GAGCTGGTGT CACTACATAA AGCAGCTGCA ACCCTGCCTA GTATGCCAAA TTTATCTGAT 
GTTCAACATC AAAATTTAAA TCAACCTGCC AAATATTCTG CGGTATCAGT CACCTCTTTA 
AAGAGCCTTG TCCCGTATGA ATCTGAAACA GATGAAGAAT CGACGGCGTC TGAGGTAAAG 
GATATATTGT TAGGTTCGGA AGATATTGAC AAGAAAAAGA AAGATATCAA AAGTGCTATT 
AAACGACCGT TGGAAAGCCC TTCACAAAAT ACCACTGCAA CCACGGAAAG TTTAAAACAA 
AAAAAGTTGG CTATTTTGAG TGAAAATGAT TTAAATACAA ACAAATCAGA CGAAACAAAT 
ACAAATCGTT CAAAGGATGA TGTAACTTCA AGTGAAGATT TCACAAATAA GACGCACGAT 
GAGGACGAAT GTGATGATCA TTTAGTTTCA TCGAGTGTAT CTCGACCACC GTTTGTACGT 
TCACATTCTA CACCGCCTTC TCCACCTGTC ATTAAAACAA AAACAGGTTT ATGGCAAGTC 
ACTACATTAC AGCCGGTGAC AGCGTGTATC AGTCCGCCGG AGAAAAAAGT GTTAAAAAAT 
GTTAAGAACC CATTCGCCAA AAAATCATTG GACACATTTA ATAAACGTGC AAAGCCCAAC 
CCGGAGGTGA AGTCTGAAAA GACCTTTGCG GGTAATGGCT ATAGATGCAG CAATGTAACC 
GATGATTCCG TATCAGGTCT ATTGAAACAG TCTCATCGTG GATATGGTGT TCCCGTTCTC 
TCATGGAAGG GAGACCAAAC AAAAATATCT AAAGAGGTGG AAATTGATGC TCAACAACAG 
CGACAAAATG ACTGGCAAAA TGATGAAGAT GCCGAAATCG ACCGTGGACG TCAGAAAAAA 
GTGAAGAAAG AAGGATCACA ATCCGATAAT CGAATTCCGG GATTCAACCC ATTTCAAGAA 
CAAGAAAATC AAAGACGATG GAGATCCAAC GGGGTAACTA ATAGGCATTA TAGTGGATAT 
GTTCGACAAC AATTCCAACG CAGCAAATTC AAGTTTCAAC GGTTCAATCC GAAATTCCAG 
CGAATGACTG CAATGTCTTA TAAGCGAAAC AATCACAGAT TGAATAGCGG TTATACACGA 
CGGAACTCAT AA