LOC105212349 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105212349
Entrez Gene ID	105212349
Full Name	DNA-directed RNA polymerase II subunit RPB2
Synonyms	RpII140_0
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011184251.1	XP_011182553.1	DNA-directed RNA polymerase II subunit RPB2
XM_011184251.2	XP_011182553.1	DNA-directed RNA polymerase II subunit RPB2

Pathways from BioSystems
Gene Ontology

The following LOC105212349 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105212349 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa198520	XM_011184251.2 Latest version!	Zeugodacus cucurbitae DNA-directed RNA polymerase II subunit RPB2 (LOC105212349), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
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OBa198520	XM_011184251.1	Bactrocera cucurbitae DNA-directed RNA polymerase II subunit RPB2 (LOC105212349), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105212349 ( XM_011184251.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011182553.1 Bactrocera cucurbitae DNA-directed RNA polymerase II subunit RPB2 (LOC105212349), mRNA.

Price & Availability↑

CloneID	OBa198520
Clone ID Related Accession (Same CDS sequence)	XM_011184251.1 , XM_011184251.2
Accession Version	XM_011184251.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTACG AAGATGATGA AATGTTCGAA GAAGAGAATG CGGAGGAAAT CTCCCACGAG 
TTGTGGCAGG AAGCTTGCTG GATTGTAATT AACGCCTATT TCGACGAAAA AGGTTTGGTA 
CGACAACAGC TGGACAGTTT CGATGAATTT ATACAAATGT CTGTGCAACG CATTGTAGAA 
GACTCCCCGG CAATTGATTT GCAAGCCGAA GCCCAACACA CATCTGGTGA AATCGAAACA 
CCCCCACGCT TTTTGCTTAA ATTTGAGCAA ATTTATTTAT CCAAACCTAC ACATTGGGAA 
AAAGATGGTT CTCCAAGCCC CATGATGCCG AACGAAGCCC GTTTACGAAA TCTGACATAT 
TCTGCTCCAC TGTATGTGGA CATAACAAAA ACAAAAATTG TGGAAGGAGC CGATCCAGTA 
GAAACACAGC ATCAGAAAAC CTTTATTGGA AAAATTCCTA TTATGTTGCG TTCTACGTAC 
TGCCTGCTTT CGCAGCTAAC AGATCGTGAT TTAACAGAAT TAAATGAGTG TCCCCTAGAT 
CCAGGTGGTT ATTTCATTAT CAACGGATCT GAAAAAGTTC TGATCGCTCA AGAGAAAATG 
GCAACCAATA CTGTATACGT ATTCAGCATG AAAGACGGCA AATATGCTTT CAAATCGGAA 
ATCCGTTCTT GCCTTGAACA CAGCTCCAGA CCCACCTCCA CTTTGTGGGT AAATATGATG 
GCACGTGGTT CACAGAACAT CAAAAAGTCT GCCATTGGCC AGCGCATAAT CGCGATCTTA 
CCATATATCA AACAGGAAAT TCCAATTATG ATAGTTTTTC GTGCTTTGGG CTTTGTAGCT 
GATCGCGATA TTTTGGAGCA CATCATTTAT GATTTCGATG ATCCCGAAAT GATGGAAATG 
GTAAAACCAT CACTTGACGA GGCATTCGTT GTGCAAGAAC AAAATGTAGC CTTAAATTTT 
ATTGGTGCCC GCGGTGCTCG ACCGGGTGTA ACCAAAGACA AGCGTATAAA ATACGCAAAG 
GAAATTTTAC AAAAGGAAAT GTTGCCGCAT GTAGGCGTAT CCGATTTCTG TGAAACCAAG 
AAAGCATACT TTTTGGGTTA TATGGTGCAT CGTTTGCTGC TTGCTTCGCT CGGTCGTCGT 
GAATTAGATG ATCGCGATCA CTACGGCAAC AAACGTTTGG ATCTCGCCGG TCCGCTATTG 
GCCTTCCTCT TCCGTGGGCT ATTCAAAAAT CTCATGAAAG AAGTCCGCAT GTACACACAG 
AAATTCATCG ATCGTGGAAA GGATTTCAAC TTGGAATTAG CCATCAAAAC AAATATTATC 
ACTGACGGTT TGCGTTACTC TTTGGCTACT GGTAACTGGG GTGACCAAAA GAAAGCGCAT 
CAGGCGCGTG CTGGCGTGTC TCAGGTGTTG AATCGTCTAA CTTTTGCATC CACATTGTCA 
CATTTACGGC GTGTCAACTC GCCCATCGGT CGCGATGGTA AATTGGCCAA ACCACGTCAA 
TTGCACAACA CATTGTGGGG CATGTTATGC CCCGCCGAGA CACCAGAGGG TGCCGCTGTA 
GGTCTGGTAA AGAACTTGGC TCTGATGGCG TACATTTCTG TTGGTTCCCA ACCTTCGCCA 
ATTTTAGAGT TCTTGGAAGA ATGGTCCATG GAAAATCTTG AAGAAATTGC ACCCTCTGCC 
ATCGCAGATG CCACAAAAAT TTTCGTCAAT GGCTGCTGGG TTGGCATACA TCGTGACCCT 
GAGCAACTTA TGGCTACACT ACGTAAATTG CGTCGGCAGA TGGATATCAT TGTCTCGGAA 
GTATCCATGA TCCGTGATAT TCGCGATCGT GAAATACGAA TTTACACAGA CGCCGGTCGC 
ATTTGCCGTC CGCTATTGAT TGTTGAGAAT GGCTCACTTC TGTTGAAGAA GACTCATGTG 
GACATGTTGA AGGAACGTGA TTACAATAAT TATAGTTGGC AAGTTTTGGT CGCTTCGGGT 
GTCGTGGAAT ACATTGACAC CTTGGAAGAG GAGACTGTAA TGATTGCGAT GTCGCCATAC 
GACTTGAAAC AGGACAAGGA CTACGCGTAT TGTACGACCT ACACACATTG TGAAATTCAT 
CCGGCTATGA TTTTAGGCGT ATGTGCCTCT ATTATACCAT TCCCCGATCA CAATCAAAGT 
CCGCGTAACA CATATCAAAG CGCTATGGGT AAACAAGCTA TGGGTGTCTA CATTACCAAC 
TTCCACGTAC GGATGGACAC TCTGGCACAC GTACTCTATT ATCCCATGAA ACCATTGGTC 
ACTACACGTT CCATGGAATA TCTGCGTTTC AGAGAGCTAC CAGCTGGCAT TAATTCAATT 
GTTGCCATCT TATGTTACAC TGGTTACAAC CAGGAAGATT CTGTCATCTT AAATGCTTCG 
GCTGTGGAAC GTGGTTTCTT CCGATCGGTT TTCTACCGTT CGTATAAGGA TTCCGAGAAC 
AAGCGTATTG GCGATCAAGA GGAGAACTTC GAAAAGCCCA ATCGTCAGAC TTGTCAAGGA 
ATGCGCAATG CTCACTACGA CAAATTGGAT GATGACGGTA TTATTGCGCC TGGTATTCGT 
GTCTCTGGTG ACGATGTGGT TATTGGAAAA ACAATTACTC TCCCCGAAAA CGACGATGAG 
CTCGACAGTA CCACGAAACG TTTCTCTAAA CGAGATGCCT CCACTTTCCT ACGTAATTCC 
GAAACTGGTA TTGTTGATCA AGTGATGTTG ACGCTGAATA GCGAAGGTTA TAAGTTTTGC 
AAAATTCGTG TGCGTTCAGT GCGTATACCG CAAATTGGTG ACAAGTTCGC CTCGCGTCAC 
GGACAGAAGG GTACTTGTGG TATCCAGTAT CGTCAAGAAG ACATGGCGTT TACATGTGAG 
GGGCTTGCGC CTGACATTAT TATCAACCCT CACGCCATTC CATCTCGTAT GACAATTGGC 
CATTTAATCG AGTGTTTGCA AGGAAAGTTG GGCTCAAACA AAGGTGAAAT TGGTGACGCT 
ACACCCTTCA ACGATGCTGT CAACGTACAA AAGATATCCA CCTTCCTGCA AGAGTATGGC 
TATCATTTGC GTGGCAACGA GGTGATGTAC AACGGTCATA CAGGTCGCAA AATCAATGCC 
CAAGTATTCT TGGGACCAAC CTATTACCAA CGTCTCAAGC ACATGGTGGA CGACAAAATT 
CACTCCCGTG CTCGTGGTCC TGTCCAGATT TTGGTGCGTC AACCTATGGA GGGTAGGGCA 
CGTGATGGTG GTCTACGGTT CGGTGAGATG GAACGTGATT GTCAGATATC TCATGGCGCT 
GCACAATTTC TGCGCGAACG TCTTTTTGAG GTTTCCGATC CATACCGTAT ACATGTGTGC 
AATTTCTGTG GTCTCATTGC TATAGCTAAT TTGCGTAATA ACACTTTTGA ATGCAAGGGT 
TGCAAAAACA AAACTCAAAT ATCACAAGTC CGTTTGCCGT ATGCCGCAAA GTTGCTGTTC 
CAGGAACTTA TGTCGATGAA TATTGCACCG CGACTGATGG TTGTCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011182553.1
CDS	117..3647
Translation	MMYEDDEMFEEENAEEISHELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPAIDLQAEAQHTSGEIETPPRFLLKFEQIYLSKPTHWEKDGSPSPMMPNEARLRNLTYSAPLYVDITKTKIVEGADPVETQHQKTFIGKIPIMLRSTYCLLSQLTDRDLTELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFSMKDGKYAFKSEIRSCLEHSSRPTSTLWVNMMARGSQNIKKSAIGQRIIAILPYIKQEIPIMIVFRALGFVADRDILEHIIYDFDDPEMMEMVKPSLDEAFVVQEQNVALNFIGARGARPGVTKDKRIKYAKEILQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLASLGRRELDDRDHYGNKRLDLAGPLLAFLFRGLFKNLMKEVRMYTQKFIDRGKDFNLELAIKTNIITDGLRYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRVNSPIGRDGKLAKPRQLHNTLWGMLCPAETPEGAAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEIAPSAIADATKIFVNGCWVGIHRDPEQLMATLRKLRRQMDIIVSEVSMIRDIRDREIRIYTDAGRICRPLLIVENGSLLLKKTHVDMLKERDYNNYSWQVLVASGVVEYIDTLEEETVMIAMSPYDLKQDKDYAYCTTYTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPMKPLVTTRSMEYLRFRELPAGINSIVAILCYTGYNQEDSVILNASAVERGFFRSVFYRSYKDSENKRIGDQEENFEKPNRQTCQGMRNAHYDKLDDDGIIAPGIRVSGDDVVIGKTITLPENDDELDSTTKRFSKRDASTFLRNSETGIVDQVMLTLNSEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMAFTCEGLAPDIIINPHAIPSRMTIGHLIECLQGKLGSNKGEIGDATPFNDAVNVQKISTFLQEYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQISHGAAQFLRERLFEVSDPYRIHVCNFCGLIAIANLRNNTFECKGCKNKTQISQVRLPYAAKLLFQELMSMNIAPRLMVVQ

Target ORF information:

RefSeq Version	XM_011184251.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae DNA-directed RNA polymerase II subunit RPB2 (LOC105212349), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011184251.1

ORF Insert Sequence:

ATGATGTACG AAGATGATGA AATGTTCGAA GAAGAGAATG CGGAGGAAAT CTCCCACGAG 
TTGTGGCAGG AAGCTTGCTG GATTGTAATT AACGCCTATT TCGACGAAAA AGGTTTGGTA 
CGACAACAGC TGGACAGTTT CGATGAATTT ATACAAATGT CTGTGCAACG CATTGTAGAA 
GACTCCCCGG CAATTGATTT GCAAGCCGAA GCCCAACACA CATCTGGTGA AATCGAAACA 
CCCCCACGCT TTTTGCTTAA ATTTGAGCAA ATTTATTTAT CCAAACCTAC ACATTGGGAA 
AAAGATGGTT CTCCAAGCCC CATGATGCCG AACGAAGCCC GTTTACGAAA TCTGACATAT 
TCTGCTCCAC TGTATGTGGA CATAACAAAA ACAAAAATTG TGGAAGGAGC CGATCCAGTA 
GAAACACAGC ATCAGAAAAC CTTTATTGGA AAAATTCCTA TTATGTTGCG TTCTACGTAC 
TGCCTGCTTT CGCAGCTAAC AGATCGTGAT TTAACAGAAT TAAATGAGTG TCCCCTAGAT 
CCAGGTGGTT ATTTCATTAT CAACGGATCT GAAAAAGTTC TGATCGCTCA AGAGAAAATG 
GCAACCAATA CTGTATACGT ATTCAGCATG AAAGACGGCA AATATGCTTT CAAATCGGAA 
ATCCGTTCTT GCCTTGAACA CAGCTCCAGA CCCACCTCCA CTTTGTGGGT AAATATGATG 
GCACGTGGTT CACAGAACAT CAAAAAGTCT GCCATTGGCC AGCGCATAAT CGCGATCTTA 
CCATATATCA AACAGGAAAT TCCAATTATG ATAGTTTTTC GTGCTTTGGG CTTTGTAGCT 
GATCGCGATA TTTTGGAGCA CATCATTTAT GATTTCGATG ATCCCGAAAT GATGGAAATG 
GTAAAACCAT CACTTGACGA GGCATTCGTT GTGCAAGAAC AAAATGTAGC CTTAAATTTT 
ATTGGTGCCC GCGGTGCTCG ACCGGGTGTA ACCAAAGACA AGCGTATAAA ATACGCAAAG 
GAAATTTTAC AAAAGGAAAT GTTGCCGCAT GTAGGCGTAT CCGATTTCTG TGAAACCAAG 
AAAGCATACT TTTTGGGTTA TATGGTGCAT CGTTTGCTGC TTGCTTCGCT CGGTCGTCGT 
GAATTAGATG ATCGCGATCA CTACGGCAAC AAACGTTTGG ATCTCGCCGG TCCGCTATTG 
GCCTTCCTCT TCCGTGGGCT ATTCAAAAAT CTCATGAAAG AAGTCCGCAT GTACACACAG 
AAATTCATCG ATCGTGGAAA GGATTTCAAC TTGGAATTAG CCATCAAAAC AAATATTATC 
ACTGACGGTT TGCGTTACTC TTTGGCTACT GGTAACTGGG GTGACCAAAA GAAAGCGCAT 
CAGGCGCGTG CTGGCGTGTC TCAGGTGTTG AATCGTCTAA CTTTTGCATC CACATTGTCA 
CATTTACGGC GTGTCAACTC GCCCATCGGT CGCGATGGTA AATTGGCCAA ACCACGTCAA 
TTGCACAACA CATTGTGGGG CATGTTATGC CCCGCCGAGA CACCAGAGGG TGCCGCTGTA 
GGTCTGGTAA AGAACTTGGC TCTGATGGCG TACATTTCTG TTGGTTCCCA ACCTTCGCCA 
ATTTTAGAGT TCTTGGAAGA ATGGTCCATG GAAAATCTTG AAGAAATTGC ACCCTCTGCC 
ATCGCAGATG CCACAAAAAT TTTCGTCAAT GGCTGCTGGG TTGGCATACA TCGTGACCCT 
GAGCAACTTA TGGCTACACT ACGTAAATTG CGTCGGCAGA TGGATATCAT TGTCTCGGAA 
GTATCCATGA TCCGTGATAT TCGCGATCGT GAAATACGAA TTTACACAGA CGCCGGTCGC 
ATTTGCCGTC CGCTATTGAT TGTTGAGAAT GGCTCACTTC TGTTGAAGAA GACTCATGTG 
GACATGTTGA AGGAACGTGA TTACAATAAT TATAGTTGGC AAGTTTTGGT CGCTTCGGGT 
GTCGTGGAAT ACATTGACAC CTTGGAAGAG GAGACTGTAA TGATTGCGAT GTCGCCATAC 
GACTTGAAAC AGGACAAGGA CTACGCGTAT TGTACGACCT ACACACATTG TGAAATTCAT 
CCGGCTATGA TTTTAGGCGT ATGTGCCTCT ATTATACCAT TCCCCGATCA CAATCAAAGT 
CCGCGTAACA CATATCAAAG CGCTATGGGT AAACAAGCTA TGGGTGTCTA CATTACCAAC 
TTCCACGTAC GGATGGACAC TCTGGCACAC GTACTCTATT ATCCCATGAA ACCATTGGTC 
ACTACACGTT CCATGGAATA TCTGCGTTTC AGAGAGCTAC CAGCTGGCAT TAATTCAATT 
GTTGCCATCT TATGTTACAC TGGTTACAAC CAGGAAGATT CTGTCATCTT AAATGCTTCG 
GCTGTGGAAC GTGGTTTCTT CCGATCGGTT TTCTACCGTT CGTATAAGGA TTCCGAGAAC 
AAGCGTATTG GCGATCAAGA GGAGAACTTC GAAAAGCCCA ATCGTCAGAC TTGTCAAGGA 
ATGCGCAATG CTCACTACGA CAAATTGGAT GATGACGGTA TTATTGCGCC TGGTATTCGT 
GTCTCTGGTG ACGATGTGGT TATTGGAAAA ACAATTACTC TCCCCGAAAA CGACGATGAG 
CTCGACAGTA CCACGAAACG TTTCTCTAAA CGAGATGCCT CCACTTTCCT ACGTAATTCC 
GAAACTGGTA TTGTTGATCA AGTGATGTTG ACGCTGAATA GCGAAGGTTA TAAGTTTTGC 
AAAATTCGTG TGCGTTCAGT GCGTATACCG CAAATTGGTG ACAAGTTCGC CTCGCGTCAC 
GGACAGAAGG GTACTTGTGG TATCCAGTAT CGTCAAGAAG ACATGGCGTT TACATGTGAG 
GGGCTTGCGC CTGACATTAT TATCAACCCT CACGCCATTC CATCTCGTAT GACAATTGGC 
CATTTAATCG AGTGTTTGCA AGGAAAGTTG GGCTCAAACA AAGGTGAAAT TGGTGACGCT 
ACACCCTTCA ACGATGCTGT CAACGTACAA AAGATATCCA CCTTCCTGCA AGAGTATGGC 
TATCATTTGC GTGGCAACGA GGTGATGTAC AACGGTCATA CAGGTCGCAA AATCAATGCC 
CAAGTATTCT TGGGACCAAC CTATTACCAA CGTCTCAAGC ACATGGTGGA CGACAAAATT 
CACTCCCGTG CTCGTGGTCC TGTCCAGATT TTGGTGCGTC AACCTATGGA GGGTAGGGCA 
CGTGATGGTG GTCTACGGTT CGGTGAGATG GAACGTGATT GTCAGATATC TCATGGCGCT 
GCACAATTTC TGCGCGAACG TCTTTTTGAG GTTTCCGATC CATACCGTAT ACATGTGTGC 
AATTTCTGTG GTCTCATTGC TATAGCTAAT TTGCGTAATA ACACTTTTGA ATGCAAGGGT 
TGCAAAAACA AAACTCAAAT ATCACAAGTC CGTTTGCCGT ATGCCGCAAA GTTGCTGTTC 
CAGGAACTTA TGTCGATGAA TATTGCACCG CGACTGATGG TTGTCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105212349 ( XM_011184251.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011182553.1 Zeugodacus cucurbitae DNA-directed RNA polymerase II subunit RPB2 (LOC105212349), mRNA.

Price & Availability↑

CloneID	OBa198520
Clone ID Related Accession (Same CDS sequence)	XM_011184251.1 , XM_011184251.2
Accession Version	XM_011184251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011184251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTACG AAGATGATGA AATGTTCGAA GAAGAGAATG CGGAGGAAAT CTCCCACGAG 
TTGTGGCAGG AAGCTTGCTG GATTGTAATT AACGCCTATT TCGACGAAAA AGGTTTGGTA 
CGACAACAGC TGGACAGTTT CGATGAATTT ATACAAATGT CTGTGCAACG CATTGTAGAA 
GACTCCCCGG CAATTGATTT GCAAGCCGAA GCCCAACACA CATCTGGTGA AATCGAAACA 
CCCCCACGCT TTTTGCTTAA ATTTGAGCAA ATTTATTTAT CCAAACCTAC ACATTGGGAA 
AAAGATGGTT CTCCAAGCCC CATGATGCCG AACGAAGCCC GTTTACGAAA TCTGACATAT 
TCTGCTCCAC TGTATGTGGA CATAACAAAA ACAAAAATTG TGGAAGGAGC CGATCCAGTA 
GAAACACAGC ATCAGAAAAC CTTTATTGGA AAAATTCCTA TTATGTTGCG TTCTACGTAC 
TGCCTGCTTT CGCAGCTAAC AGATCGTGAT TTAACAGAAT TAAATGAGTG TCCCCTAGAT 
CCAGGTGGTT ATTTCATTAT CAACGGATCT GAAAAAGTTC TGATCGCTCA AGAGAAAATG 
GCAACCAATA CTGTATACGT ATTCAGCATG AAAGACGGCA AATATGCTTT CAAATCGGAA 
ATCCGTTCTT GCCTTGAACA CAGCTCCAGA CCCACCTCCA CTTTGTGGGT AAATATGATG 
GCACGTGGTT CACAGAACAT CAAAAAGTCT GCCATTGGCC AGCGCATAAT CGCGATCTTA 
CCATATATCA AACAGGAAAT TCCAATTATG ATAGTTTTTC GTGCTTTGGG CTTTGTAGCT 
GATCGCGATA TTTTGGAGCA CATCATTTAT GATTTCGATG ATCCCGAAAT GATGGAAATG 
GTAAAACCAT CACTTGACGA GGCATTCGTT GTGCAAGAAC AAAATGTAGC CTTAAATTTT 
ATTGGTGCCC GCGGTGCTCG ACCGGGTGTA ACCAAAGACA AGCGTATAAA ATACGCAAAG 
GAAATTTTAC AAAAGGAAAT GTTGCCGCAT GTAGGCGTAT CCGATTTCTG TGAAACCAAG 
AAAGCATACT TTTTGGGTTA TATGGTGCAT CGTTTGCTGC TTGCTTCGCT CGGTCGTCGT 
GAATTAGATG ATCGCGATCA CTACGGCAAC AAACGTTTGG ATCTCGCCGG TCCGCTATTG 
GCCTTCCTCT TCCGTGGGCT ATTCAAAAAT CTCATGAAAG AAGTCCGCAT GTACACACAG 
AAATTCATCG ATCGTGGAAA GGATTTCAAC TTGGAATTAG CCATCAAAAC AAATATTATC 
ACTGACGGTT TGCGTTACTC TTTGGCTACT GGTAACTGGG GTGACCAAAA GAAAGCGCAT 
CAGGCGCGTG CTGGCGTGTC TCAGGTGTTG AATCGTCTAA CTTTTGCATC CACATTGTCA 
CATTTACGGC GTGTCAACTC GCCCATCGGT CGCGATGGTA AATTGGCCAA ACCACGTCAA 
TTGCACAACA CATTGTGGGG CATGTTATGC CCCGCCGAGA CACCAGAGGG TGCCGCTGTA 
GGTCTGGTAA AGAACTTGGC TCTGATGGCG TACATTTCTG TTGGTTCCCA ACCTTCGCCA 
ATTTTAGAGT TCTTGGAAGA ATGGTCCATG GAAAATCTTG AAGAAATTGC ACCCTCTGCC 
ATCGCAGATG CCACAAAAAT TTTCGTCAAT GGCTGCTGGG TTGGCATACA TCGTGACCCT 
GAGCAACTTA TGGCTACACT ACGTAAATTG CGTCGGCAGA TGGATATCAT TGTCTCGGAA 
GTATCCATGA TCCGTGATAT TCGCGATCGT GAAATACGAA TTTACACAGA CGCCGGTCGC 
ATTTGCCGTC CGCTATTGAT TGTTGAGAAT GGCTCACTTC TGTTGAAGAA GACTCATGTG 
GACATGTTGA AGGAACGTGA TTACAATAAT TATAGTTGGC AAGTTTTGGT CGCTTCGGGT 
GTCGTGGAAT ACATTGACAC CTTGGAAGAG GAGACTGTAA TGATTGCGAT GTCGCCATAC 
GACTTGAAAC AGGACAAGGA CTACGCGTAT TGTACGACCT ACACACATTG TGAAATTCAT 
CCGGCTATGA TTTTAGGCGT ATGTGCCTCT ATTATACCAT TCCCCGATCA CAATCAAAGT 
CCGCGTAACA CATATCAAAG CGCTATGGGT AAACAAGCTA TGGGTGTCTA CATTACCAAC 
TTCCACGTAC GGATGGACAC TCTGGCACAC GTACTCTATT ATCCCATGAA ACCATTGGTC 
ACTACACGTT CCATGGAATA TCTGCGTTTC AGAGAGCTAC CAGCTGGCAT TAATTCAATT 
GTTGCCATCT TATGTTACAC TGGTTACAAC CAGGAAGATT CTGTCATCTT AAATGCTTCG 
GCTGTGGAAC GTGGTTTCTT CCGATCGGTT TTCTACCGTT CGTATAAGGA TTCCGAGAAC 
AAGCGTATTG GCGATCAAGA GGAGAACTTC GAAAAGCCCA ATCGTCAGAC TTGTCAAGGA 
ATGCGCAATG CTCACTACGA CAAATTGGAT GATGACGGTA TTATTGCGCC TGGTATTCGT 
GTCTCTGGTG ACGATGTGGT TATTGGAAAA ACAATTACTC TCCCCGAAAA CGACGATGAG 
CTCGACAGTA CCACGAAACG TTTCTCTAAA CGAGATGCCT CCACTTTCCT ACGTAATTCC 
GAAACTGGTA TTGTTGATCA AGTGATGTTG ACGCTGAATA GCGAAGGTTA TAAGTTTTGC 
AAAATTCGTG TGCGTTCAGT GCGTATACCG CAAATTGGTG ACAAGTTCGC CTCGCGTCAC 
GGACAGAAGG GTACTTGTGG TATCCAGTAT CGTCAAGAAG ACATGGCGTT TACATGTGAG 
GGGCTTGCGC CTGACATTAT TATCAACCCT CACGCCATTC CATCTCGTAT GACAATTGGC 
CATTTAATCG AGTGTTTGCA AGGAAAGTTG GGCTCAAACA AAGGTGAAAT TGGTGACGCT 
ACACCCTTCA ACGATGCTGT CAACGTACAA AAGATATCCA CCTTCCTGCA AGAGTATGGC 
TATCATTTGC GTGGCAACGA GGTGATGTAC AACGGTCATA CAGGTCGCAA AATCAATGCC 
CAAGTATTCT TGGGACCAAC CTATTACCAA CGTCTCAAGC ACATGGTGGA CGACAAAATT 
CACTCCCGTG CTCGTGGTCC TGTCCAGATT TTGGTGCGTC AACCTATGGA GGGTAGGGCA 
CGTGATGGTG GTCTACGGTT CGGTGAGATG GAACGTGATT GTCAGATATC TCATGGCGCT 
GCACAATTTC TGCGCGAACG TCTTTTTGAG GTTTCCGATC CATACCGTAT ACATGTGTGC 
AATTTCTGTG GTCTCATTGC TATAGCTAAT TTGCGTAATA ACACTTTTGA ATGCAAGGGT 
TGCAAAAACA AAACTCAAAT ATCACAAGTC CGTTTGCCGT ATGCCGCAAA GTTGCTGTTC 
CAGGAACTTA TGTCGATGAA TATTGCACCG CGACTGATGG TTGTCCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011182553.1
CDS	145..3675
Translation	MMYEDDEMFEEENAEEISHELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIVEDSPAIDLQAEAQHTSGEIETPPRFLLKFEQIYLSKPTHWEKDGSPSPMMPNEARLRNLTYSAPLYVDITKTKIVEGADPVETQHQKTFIGKIPIMLRSTYCLLSQLTDRDLTELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFSMKDGKYAFKSEIRSCLEHSSRPTSTLWVNMMARGSQNIKKSAIGQRIIAILPYIKQEIPIMIVFRALGFVADRDILEHIIYDFDDPEMMEMVKPSLDEAFVVQEQNVALNFIGARGARPGVTKDKRIKYAKEILQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLASLGRRELDDRDHYGNKRLDLAGPLLAFLFRGLFKNLMKEVRMYTQKFIDRGKDFNLELAIKTNIITDGLRYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRVNSPIGRDGKLAKPRQLHNTLWGMLCPAETPEGAAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEIAPSAIADATKIFVNGCWVGIHRDPEQLMATLRKLRRQMDIIVSEVSMIRDIRDREIRIYTDAGRICRPLLIVENGSLLLKKTHVDMLKERDYNNYSWQVLVASGVVEYIDTLEEETVMIAMSPYDLKQDKDYAYCTTYTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPMKPLVTTRSMEYLRFRELPAGINSIVAILCYTGYNQEDSVILNASAVERGFFRSVFYRSYKDSENKRIGDQEENFEKPNRQTCQGMRNAHYDKLDDDGIIAPGIRVSGDDVVIGKTITLPENDDELDSTTKRFSKRDASTFLRNSETGIVDQVMLTLNSEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMAFTCEGLAPDIIINPHAIPSRMTIGHLIECLQGKLGSNKGEIGDATPFNDAVNVQKISTFLQEYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQISHGAAQFLRERLFEVSDPYRIHVCNFCGLIAIANLRNNTFECKGCKNKTQISQVRLPYAAKLLFQELMSMNIAPRLMVVQ

Target ORF information:

RefSeq Version	XM_011184251.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae DNA-directed RNA polymerase II subunit RPB2 (LOC105212349), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011184251.2

ORF Insert Sequence:

ATGATGTACG AAGATGATGA AATGTTCGAA GAAGAGAATG CGGAGGAAAT CTCCCACGAG 
TTGTGGCAGG AAGCTTGCTG GATTGTAATT AACGCCTATT TCGACGAAAA AGGTTTGGTA 
CGACAACAGC TGGACAGTTT CGATGAATTT ATACAAATGT CTGTGCAACG CATTGTAGAA 
GACTCCCCGG CAATTGATTT GCAAGCCGAA GCCCAACACA CATCTGGTGA AATCGAAACA 
CCCCCACGCT TTTTGCTTAA ATTTGAGCAA ATTTATTTAT CCAAACCTAC ACATTGGGAA 
AAAGATGGTT CTCCAAGCCC CATGATGCCG AACGAAGCCC GTTTACGAAA TCTGACATAT 
TCTGCTCCAC TGTATGTGGA CATAACAAAA ACAAAAATTG TGGAAGGAGC CGATCCAGTA 
GAAACACAGC ATCAGAAAAC CTTTATTGGA AAAATTCCTA TTATGTTGCG TTCTACGTAC 
TGCCTGCTTT CGCAGCTAAC AGATCGTGAT TTAACAGAAT TAAATGAGTG TCCCCTAGAT 
CCAGGTGGTT ATTTCATTAT CAACGGATCT GAAAAAGTTC TGATCGCTCA AGAGAAAATG 
GCAACCAATA CTGTATACGT ATTCAGCATG AAAGACGGCA AATATGCTTT CAAATCGGAA 
ATCCGTTCTT GCCTTGAACA CAGCTCCAGA CCCACCTCCA CTTTGTGGGT AAATATGATG 
GCACGTGGTT CACAGAACAT CAAAAAGTCT GCCATTGGCC AGCGCATAAT CGCGATCTTA 
CCATATATCA AACAGGAAAT TCCAATTATG ATAGTTTTTC GTGCTTTGGG CTTTGTAGCT 
GATCGCGATA TTTTGGAGCA CATCATTTAT GATTTCGATG ATCCCGAAAT GATGGAAATG 
GTAAAACCAT CACTTGACGA GGCATTCGTT GTGCAAGAAC AAAATGTAGC CTTAAATTTT 
ATTGGTGCCC GCGGTGCTCG ACCGGGTGTA ACCAAAGACA AGCGTATAAA ATACGCAAAG 
GAAATTTTAC AAAAGGAAAT GTTGCCGCAT GTAGGCGTAT CCGATTTCTG TGAAACCAAG 
AAAGCATACT TTTTGGGTTA TATGGTGCAT CGTTTGCTGC TTGCTTCGCT CGGTCGTCGT 
GAATTAGATG ATCGCGATCA CTACGGCAAC AAACGTTTGG ATCTCGCCGG TCCGCTATTG 
GCCTTCCTCT TCCGTGGGCT ATTCAAAAAT CTCATGAAAG AAGTCCGCAT GTACACACAG 
AAATTCATCG ATCGTGGAAA GGATTTCAAC TTGGAATTAG CCATCAAAAC AAATATTATC 
ACTGACGGTT TGCGTTACTC TTTGGCTACT GGTAACTGGG GTGACCAAAA GAAAGCGCAT 
CAGGCGCGTG CTGGCGTGTC TCAGGTGTTG AATCGTCTAA CTTTTGCATC CACATTGTCA 
CATTTACGGC GTGTCAACTC GCCCATCGGT CGCGATGGTA AATTGGCCAA ACCACGTCAA 
TTGCACAACA CATTGTGGGG CATGTTATGC CCCGCCGAGA CACCAGAGGG TGCCGCTGTA 
GGTCTGGTAA AGAACTTGGC TCTGATGGCG TACATTTCTG TTGGTTCCCA ACCTTCGCCA 
ATTTTAGAGT TCTTGGAAGA ATGGTCCATG GAAAATCTTG AAGAAATTGC ACCCTCTGCC 
ATCGCAGATG CCACAAAAAT TTTCGTCAAT GGCTGCTGGG TTGGCATACA TCGTGACCCT 
GAGCAACTTA TGGCTACACT ACGTAAATTG CGTCGGCAGA TGGATATCAT TGTCTCGGAA 
GTATCCATGA TCCGTGATAT TCGCGATCGT GAAATACGAA TTTACACAGA CGCCGGTCGC 
ATTTGCCGTC CGCTATTGAT TGTTGAGAAT GGCTCACTTC TGTTGAAGAA GACTCATGTG 
GACATGTTGA AGGAACGTGA TTACAATAAT TATAGTTGGC AAGTTTTGGT CGCTTCGGGT 
GTCGTGGAAT ACATTGACAC CTTGGAAGAG GAGACTGTAA TGATTGCGAT GTCGCCATAC 
GACTTGAAAC AGGACAAGGA CTACGCGTAT TGTACGACCT ACACACATTG TGAAATTCAT 
CCGGCTATGA TTTTAGGCGT ATGTGCCTCT ATTATACCAT TCCCCGATCA CAATCAAAGT 
CCGCGTAACA CATATCAAAG CGCTATGGGT AAACAAGCTA TGGGTGTCTA CATTACCAAC 
TTCCACGTAC GGATGGACAC TCTGGCACAC GTACTCTATT ATCCCATGAA ACCATTGGTC 
ACTACACGTT CCATGGAATA TCTGCGTTTC AGAGAGCTAC CAGCTGGCAT TAATTCAATT 
GTTGCCATCT TATGTTACAC TGGTTACAAC CAGGAAGATT CTGTCATCTT AAATGCTTCG 
GCTGTGGAAC GTGGTTTCTT CCGATCGGTT TTCTACCGTT CGTATAAGGA TTCCGAGAAC 
AAGCGTATTG GCGATCAAGA GGAGAACTTC GAAAAGCCCA ATCGTCAGAC TTGTCAAGGA 
ATGCGCAATG CTCACTACGA CAAATTGGAT GATGACGGTA TTATTGCGCC TGGTATTCGT 
GTCTCTGGTG ACGATGTGGT TATTGGAAAA ACAATTACTC TCCCCGAAAA CGACGATGAG 
CTCGACAGTA CCACGAAACG TTTCTCTAAA CGAGATGCCT CCACTTTCCT ACGTAATTCC 
GAAACTGGTA TTGTTGATCA AGTGATGTTG ACGCTGAATA GCGAAGGTTA TAAGTTTTGC 
AAAATTCGTG TGCGTTCAGT GCGTATACCG CAAATTGGTG ACAAGTTCGC CTCGCGTCAC 
GGACAGAAGG GTACTTGTGG TATCCAGTAT CGTCAAGAAG ACATGGCGTT TACATGTGAG 
GGGCTTGCGC CTGACATTAT TATCAACCCT CACGCCATTC CATCTCGTAT GACAATTGGC 
CATTTAATCG AGTGTTTGCA AGGAAAGTTG GGCTCAAACA AAGGTGAAAT TGGTGACGCT 
ACACCCTTCA ACGATGCTGT CAACGTACAA AAGATATCCA CCTTCCTGCA AGAGTATGGC 
TATCATTTGC GTGGCAACGA GGTGATGTAC AACGGTCATA CAGGTCGCAA AATCAATGCC 
CAAGTATTCT TGGGACCAAC CTATTACCAA CGTCTCAAGC ACATGGTGGA CGACAAAATT 
CACTCCCGTG CTCGTGGTCC TGTCCAGATT TTGGTGCGTC AACCTATGGA GGGTAGGGCA 
CGTGATGGTG GTCTACGGTT CGGTGAGATG GAACGTGATT GTCAGATATC TCATGGCGCT 
GCACAATTTC TGCGCGAACG TCTTTTTGAG GTTTCCGATC CATACCGTAT ACATGTGTGC 
AATTTCTGTG GTCTCATTGC TATAGCTAAT TTGCGTAATA ACACTTTTGA ATGCAAGGGT 
TGCAAAAACA AAACTCAAAT ATCACAAGTC CGTTTGCCGT ATGCCGCAAA GTTGCTGTTC 
CAGGAACTTA TGTCGATGAA TATTGCACCG CGACTGATGG TTGTCCAATA G