LOC105208515 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105208515
Entrez Gene ID	105208515
Full Name	zinc finger protein, X-linked
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011178402.1	XP_011176704.1	zinc finger protein 91
XM_011178402.2	XP_011176704.2	zinc finger protein 26

Pathways from BioSystems
Gene Ontology

The following LOC105208515 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105208515 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa194213	XM_011178402.1 Latest version!	Bactrocera cucurbitae zinc finger protein 91 (LOC105208515), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa254473	XM_011178402.2 Latest version!	Zeugodacus cucurbitae zinc finger protein 26 (LOC105208515), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105208515 ( XM_011178402.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176704.1 Bactrocera cucurbitae zinc finger protein 91 (LOC105208515), mRNA.

Price & Availability↑

CloneID	OBa194213
Clone ID Related Accession (Same CDS sequence)	XM_011178402.1
Accession Version	XM_011178402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	zinc finger protein 91
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGAAC CCGATTTGGA CGACACACAT CTCTGCATCA AATGCAGCAC TACAATTGTG 
GGACTAGACG CTTACATCGA GCACCGTAAA ACGCAATGCA CCAACCTCAA TAGTACAGCT 
GCAAGTAGTA AGCGAGAGCA CACGACTACG CATACCGTGC ACACGGAAAT TATAACCACG 
CCACGTGCAC ATCTCGATCA CACCTATGAT GCTTTCGCCT TTGCCGAACC GGAGTCATAC 
GCCAAGGAGT CGGCACATGG GGGTAAAACC TCGAAATCGC TGTCCGACGC ATACGAGCTG 
CCATACGAGC TAGGGGCGGA TGTGTTCTTC TCATCACTGC AGCTGCAAAG TGTGCAAGCG 
GCCGGACCCT CAACGAGTGG TAAGCAGGGA CTGAGCGCAC CGAGTCAACC GCTGAGCAGT 
CTGCAACAGC GACACAACAA AACCGAGCAT AACGTACATA TCGGTGCCGC AGATGAGCCG 
TGGATGTCAG ATGCACATGC AGGTGGTAGT AATGTAGAGC AGCAGCAAAA ATCTTACGCA 
GTTTTCAAAC CCATCACATT CGAGCATGAA TCACCCGAGG CGAGTGAAGA AGAAGAAGAG 
GATGTTGATG TCGAAATGGA CGACGATGAT GATGATTACG ATCCAATAGC AGATGCGCAT 
GCGAATGGTG GTAAATGGAA ACCACCAACA ATGCGTCACT CACCGCCCGT AGTGCCCGAA 
ACGCATACTG GTGGCAAATG GCGACCCGAA TTTAGACCAG AATTGAATGT TACACACGCA 
CAACTCACAC GCATTTCGCC GAACTGGGAT GAAGCTGTCG AAGAGATGGA AAACGTGGAA 
GTGCACGAAG GCCAACATCC GCCTGCCGAA CACACGAAAG GTAAATGGAT ACCCGGTACG 
AAGTTGCAGC GACTCGAATA TAAAGAGGAA GTGGTGGAAT TGGCGAAATC GACGAATCAG 
GCGTACTGGT GTAATATCTG TTGTCGACGT TTGAAGAGCA AAACAAATTA TGAGAACCAT 
CTAAAGACAG GTTATCATCG CAAACGTGCG GAACCCGAAC GGCAGCTCGA AAAGGCGGCG 
CTGGGCGATA TACAACGTGT TGAATTGAGT AAAAGTTTAT TGGCTGCACT TGAGGAACCC 
AAACCAGCGA CCAGCAGTAG TGTTGTTGTA AAGAAGCGTA AGCGACGCGC CACTTTCATT 
AAATGTCAAC TCTGCAAGCA CAGCATGTTG CGCCATCTAG TGGGTAAGCA TTTGATATCG 
CACTATCACT ATCGACGCAT GCAGCTGAAT CCCGCGAAGT CACTGCACAT GATACTAGAC 
AATATACACT CGGTGGTACT GCAGTCACCG TTTCAATGTC GCCCATGTCG CTTCTACACC 
AACACCGAAG AGATGTTCCT GCAGCACTGG AGCTCGCCCA CACACTTGGA CGCCACCGAG 
GGACTCGGCA AGTTTTGGTG TAGTTATTGT CACTTCGAAT GCGAGGACAA CAATCAAATG 
CGTCGCCATC TGCTCAGCGC CGAACACAAG GAAGTCATAT TGGCCATTAA TCGTTCCGTA 
CCCATTTGCA TTAGCAAAAA GCTGAGCATC GACTGCCAGC GTTGTCGTCA GAGTTTCCGC 
TATAATGCCG AGTTACGTCG CCACGCCTTG TCTTGCCATC CCGGCAAGGA ATTGTGCGGC 
ACCGCATCGG ACGGCTATCA GTGTCGTTAC AAGTGTGAAT TATGCGAGGA CAGTTTTCGC 
ACGAAGGTCG CACTGCAACG GCACGAGAAG AACAAACATA GCGTGAGTAA ATATTTCTGT 
TCCATATGCA AATTGGAATT CAATACGCCT TGCAAGGCGC GCAGACATCG CAAAACGTTG 
GAGCATCGGA ATTGCGCAGC GGCTGCCGAA AAGAATGCAG GTGTGCGACG TGTGGCGGCG 
GTGAAGGACG TCAAGAGTTG TGATAAATGC AATTTCGTCG CTTCTTCCGA GGTTCAAGCT 
ATGCTGCACG AGCTCTCACA TCACAAGAGC GGTATTACCA TGCATAATAC AGTTATAACC 
AAAGCTAGTA CTAACAGTAA AAACAGCGAG AAGTCGCAAA AAGTCTGTGA AAAACCCAAA 
CTCAAGTGCA AAGATTGCGA AGAACTCTTC GCCGATAAGG AGGCACTCAA AACGCATCGG 
GACTCCAAAC ACCCACTATT GTGTCACGTC TGTTTGAGTT GTGGTCAAAG TTTTGCGTTG 
GCACAAGCGC TCGGCCGTCA TACACGCAAC TGTCAACCGA AGCCGAGTAC CTCAAAGGAA 
GCGCAGGCTA CTCGGCGTGA TGCGGAAAAT AGCTGGTGCT GCGACAAGTG TGACTTTAGC 
GCTCCTTACG AATCGGAACT AATCTTCCAT CGTCTGCATC ACACCCTTGG TCCTATGGGT 
AAAAATCAAG TTATCGCTTG TCCAATATGT ACGAAAGAGT TTCGTAAGCA TTCGTTACGT 
TGTCATCTGC GACAACACAC GAATGAGAAG ATTTTCGCTT GTGAATTCTG TGAAATGAAA 
TTTTCACGTC GTCACAATCT CAAGGATCAC ATGAAGACTG TACATAATGT GTCAGCAGCA 
GCAACAACAG GTCCATCAGC AGACATGGAT GCCCAAAAAG CGAAGAAAAG AAAAGTTGAT 
GGTGACAAAT TTGCTTGTGG TGTTTGCTCC AAGGCATTTA GCTCAGATTA TATGCTGAAA 
AAGCATAGTG CGAGCCACGC CACTTTGCCG TCGGAAAATG CTGAAAATAC TTGTAAATAT 
GAGAATTGCA AATATGTTGC CGCAAATGCC ACTTTACTGC GCGTTCATGC GGCATCACAT 
GCCAAGGAGC AGCTAAAATG CACTGAGCCA TCATGCAACT ATGAAGGGAA AAGTTTGCTG 
CATTTAAAGA GACACTCCCG CACCCACCTC GCGCCATCCA AATGGTTTAC ATGTGACCAA 
TGCGAATTCA AGGCGCGCAT CAAGGGACAT CTAAAACGTC ATCTGCGCAC TCACACTGGT 
GAAAAGCCGT TCCACTGTCG ACACTGTGAG TTCCGCTGCA GTACTTTCGA TAATTTGCGT 
AAACATGTCC TCAAAACCGG CAAACATCCA GGCAAATTCA TATACGAATG TGAGCATTGC 
AATAATGCTG AAGAGAAGAA ATTCTTCAAA ACCAATTCAT ATCACGAGTT TCAAGCGCAC 
AACACAGAGA TGCATAAGAA TGCAGTGCTG GGTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176704.1
CDS	262..3480
Translation	MIEPDLDDTHLCIKCSTTIVGLDAYIEHRKTQCTNLNSTAASSKREHTTTHTVHTEIITTPRAHLDHTYDAFAFAEPESYAKESAHGGKTSKSLSDAYELPYELGADVFFSSLQLQSVQAAGPSTSGKQGLSAPSQPLSSLQQRHNKTEHNVHIGAADEPWMSDAHAGGSNVEQQQKSYAVFKPITFEHESPEASEEEEEDVDVEMDDDDDDYDPIADAHANGGKWKPPTMRHSPPVVPETHTGGKWRPEFRPELNVTHAQLTRISPNWDEAVEEMENVEVHEGQHPPAEHTKGKWIPGTKLQRLEYKEEVVELAKSTNQAYWCNICCRRLKSKTNYENHLKTGYHRKRAEPERQLEKAALGDIQRVELSKSLLAALEEPKPATSSSVVVKKRKRRATFIKCQLCKHSMLRHLVGKHLISHYHYRRMQLNPAKSLHMILDNIHSVVLQSPFQCRPCRFYTNTEEMFLQHWSSPTHLDATEGLGKFWCSYCHFECEDNNQMRRHLLSAEHKEVILAINRSVPICISKKLSIDCQRCRQSFRYNAELRRHALSCHPGKELCGTASDGYQCRYKCELCEDSFRTKVALQRHEKNKHSVSKYFCSICKLEFNTPCKARRHRKTLEHRNCAAAAEKNAGVRRVAAVKDVKSCDKCNFVASSEVQAMLHELSHHKSGITMHNTVITKASTNSKNSEKSQKVCEKPKLKCKDCEELFADKEALKTHRDSKHPLLCHVCLSCGQSFALAQALGRHTRNCQPKPSTSKEAQATRRDAENSWCCDKCDFSAPYESELIFHRLHHTLGPMGKNQVIACPICTKEFRKHSLRCHLRQHTNEKIFACEFCEMKFSRRHNLKDHMKTVHNVSAAATTGPSADMDAQKAKKRKVDGDKFACGVCSKAFSSDYMLKKHSASHATLPSENAENTCKYENCKYVAANATLLRVHAASHAKEQLKCTEPSCNYEGKSLLHLKRHSRTHLAPSKWFTCDQCEFKARIKGHLKRHLRTHTGEKPFHCRHCEFRCSTFDNLRKHVLKTGKHPGKFIYECEHCNNAEEKKFFKTNSYHEFQAHNTEMHKNAVLGT

Target ORF information:

RefSeq Version	XM_011178402.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae zinc finger protein 91 (LOC105208515), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178402.1

ORF Insert Sequence:

ATGATCGAAC CCGATTTGGA CGACACACAT CTCTGCATCA AATGCAGCAC TACAATTGTG 
GGACTAGACG CTTACATCGA GCACCGTAAA ACGCAATGCA CCAACCTCAA TAGTACAGCT 
GCAAGTAGTA AGCGAGAGCA CACGACTACG CATACCGTGC ACACGGAAAT TATAACCACG 
CCACGTGCAC ATCTCGATCA CACCTATGAT GCTTTCGCCT TTGCCGAACC GGAGTCATAC 
GCCAAGGAGT CGGCACATGG GGGTAAAACC TCGAAATCGC TGTCCGACGC ATACGAGCTG 
CCATACGAGC TAGGGGCGGA TGTGTTCTTC TCATCACTGC AGCTGCAAAG TGTGCAAGCG 
GCCGGACCCT CAACGAGTGG TAAGCAGGGA CTGAGCGCAC CGAGTCAACC GCTGAGCAGT 
CTGCAACAGC GACACAACAA AACCGAGCAT AACGTACATA TCGGTGCCGC AGATGAGCCG 
TGGATGTCAG ATGCACATGC AGGTGGTAGT AATGTAGAGC AGCAGCAAAA ATCTTACGCA 
GTTTTCAAAC CCATCACATT CGAGCATGAA TCACCCGAGG CGAGTGAAGA AGAAGAAGAG 
GATGTTGATG TCGAAATGGA CGACGATGAT GATGATTACG ATCCAATAGC AGATGCGCAT 
GCGAATGGTG GTAAATGGAA ACCACCAACA ATGCGTCACT CACCGCCCGT AGTGCCCGAA 
ACGCATACTG GTGGCAAATG GCGACCCGAA TTTAGACCAG AATTGAATGT TACACACGCA 
CAACTCACAC GCATTTCGCC GAACTGGGAT GAAGCTGTCG AAGAGATGGA AAACGTGGAA 
GTGCACGAAG GCCAACATCC GCCTGCCGAA CACACGAAAG GTAAATGGAT ACCCGGTACG 
AAGTTGCAGC GACTCGAATA TAAAGAGGAA GTGGTGGAAT TGGCGAAATC GACGAATCAG 
GCGTACTGGT GTAATATCTG TTGTCGACGT TTGAAGAGCA AAACAAATTA TGAGAACCAT 
CTAAAGACAG GTTATCATCG CAAACGTGCG GAACCCGAAC GGCAGCTCGA AAAGGCGGCG 
CTGGGCGATA TACAACGTGT TGAATTGAGT AAAAGTTTAT TGGCTGCACT TGAGGAACCC 
AAACCAGCGA CCAGCAGTAG TGTTGTTGTA AAGAAGCGTA AGCGACGCGC CACTTTCATT 
AAATGTCAAC TCTGCAAGCA CAGCATGTTG CGCCATCTAG TGGGTAAGCA TTTGATATCG 
CACTATCACT ATCGACGCAT GCAGCTGAAT CCCGCGAAGT CACTGCACAT GATACTAGAC 
AATATACACT CGGTGGTACT GCAGTCACCG TTTCAATGTC GCCCATGTCG CTTCTACACC 
AACACCGAAG AGATGTTCCT GCAGCACTGG AGCTCGCCCA CACACTTGGA CGCCACCGAG 
GGACTCGGCA AGTTTTGGTG TAGTTATTGT CACTTCGAAT GCGAGGACAA CAATCAAATG 
CGTCGCCATC TGCTCAGCGC CGAACACAAG GAAGTCATAT TGGCCATTAA TCGTTCCGTA 
CCCATTTGCA TTAGCAAAAA GCTGAGCATC GACTGCCAGC GTTGTCGTCA GAGTTTCCGC 
TATAATGCCG AGTTACGTCG CCACGCCTTG TCTTGCCATC CCGGCAAGGA ATTGTGCGGC 
ACCGCATCGG ACGGCTATCA GTGTCGTTAC AAGTGTGAAT TATGCGAGGA CAGTTTTCGC 
ACGAAGGTCG CACTGCAACG GCACGAGAAG AACAAACATA GCGTGAGTAA ATATTTCTGT 
TCCATATGCA AATTGGAATT CAATACGCCT TGCAAGGCGC GCAGACATCG CAAAACGTTG 
GAGCATCGGA ATTGCGCAGC GGCTGCCGAA AAGAATGCAG GTGTGCGACG TGTGGCGGCG 
GTGAAGGACG TCAAGAGTTG TGATAAATGC AATTTCGTCG CTTCTTCCGA GGTTCAAGCT 
ATGCTGCACG AGCTCTCACA TCACAAGAGC GGTATTACCA TGCATAATAC AGTTATAACC 
AAAGCTAGTA CTAACAGTAA AAACAGCGAG AAGTCGCAAA AAGTCTGTGA AAAACCCAAA 
CTCAAGTGCA AAGATTGCGA AGAACTCTTC GCCGATAAGG AGGCACTCAA AACGCATCGG 
GACTCCAAAC ACCCACTATT GTGTCACGTC TGTTTGAGTT GTGGTCAAAG TTTTGCGTTG 
GCACAAGCGC TCGGCCGTCA TACACGCAAC TGTCAACCGA AGCCGAGTAC CTCAAAGGAA 
GCGCAGGCTA CTCGGCGTGA TGCGGAAAAT AGCTGGTGCT GCGACAAGTG TGACTTTAGC 
GCTCCTTACG AATCGGAACT AATCTTCCAT CGTCTGCATC ACACCCTTGG TCCTATGGGT 
AAAAATCAAG TTATCGCTTG TCCAATATGT ACGAAAGAGT TTCGTAAGCA TTCGTTACGT 
TGTCATCTGC GACAACACAC GAATGAGAAG ATTTTCGCTT GTGAATTCTG TGAAATGAAA 
TTTTCACGTC GTCACAATCT CAAGGATCAC ATGAAGACTG TACATAATGT GTCAGCAGCA 
GCAACAACAG GTCCATCAGC AGACATGGAT GCCCAAAAAG CGAAGAAAAG AAAAGTTGAT 
GGTGACAAAT TTGCTTGTGG TGTTTGCTCC AAGGCATTTA GCTCAGATTA TATGCTGAAA 
AAGCATAGTG CGAGCCACGC CACTTTGCCG TCGGAAAATG CTGAAAATAC TTGTAAATAT 
GAGAATTGCA AATATGTTGC CGCAAATGCC ACTTTACTGC GCGTTCATGC GGCATCACAT 
GCCAAGGAGC AGCTAAAATG CACTGAGCCA TCATGCAACT ATGAAGGGAA AAGTTTGCTG 
CATTTAAAGA GACACTCCCG CACCCACCTC GCGCCATCCA AATGGTTTAC ATGTGACCAA 
TGCGAATTCA AGGCGCGCAT CAAGGGACAT CTAAAACGTC ATCTGCGCAC TCACACTGGT 
GAAAAGCCGT TCCACTGTCG ACACTGTGAG TTCCGCTGCA GTACTTTCGA TAATTTGCGT 
AAACATGTCC TCAAAACCGG CAAACATCCA GGCAAATTCA TATACGAATG TGAGCATTGC 
AATAATGCTG AAGAGAAGAA ATTCTTCAAA ACCAATTCAT ATCACGAGTT TCAAGCGCAC 
AACACAGAGA TGCATAAGAA TGCAGTGCTG GGTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208515 ( XM_011178402.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176704.2 Zeugodacus cucurbitae zinc finger protein 26 (LOC105208515), mRNA.

Price & Availability↑

CloneID	OBa254473
Clone ID Related Accession (Same CDS sequence)	XM_011178402.2
Accession Version	XM_011178402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	zinc finger protein 26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863673.1) and transcript sequence (GBXI01003837.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011178402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 99 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATCGAAC CCGATTTGGA CGACACACAT CTCTGCATCA AATGCAGCAC TACAATTGTG 
GGACTAGACG CTTACATCGA GCACCGTAAA ACGCAATGCA CCAACCTCAA TAGTACAGCT 
GCAAGTAGTA AGCGAGAGCA CACGACTACG CATACCGTGC ACACGGAAAT TATAACCACG 
CCACGTGCAC ATCTCGATCA CACCTATGAT GCTTTCGCCT TTGCCGAACC GGAGTCATAC 
GCCAAGGAGT CGGCACATGG GGGTAAAACC TCGAAATCGC TGTCCGACGC ATACGAGCTG 
CCATACGAGC TAGGGGCGGA TGTGTTCTTC TCATCACTGC AGCTGCAAAG TGTGCAAGCG 
GCCGGACCCT CAACGAGTGG TAAGCAGGGA CTGAGCGCAC CGAGTCAACC GCTGAGCAGT 
CTGCAACAGC GACACAACAA AACCGAGCAT AACGTACATA TCGGTGCCGC AGATGAGCCG 
TGGATGTCAG ATGCACATGC AGGTGGTAGT AATGTAGAGC AGCAGCAAAA ATCTTACGCA 
GTTTTCAAAC CCATCACATT CGAGCATGAA TCACCCGAGG CGAGTGAAGA AGAAGAAGAG 
GATGTTGATG TCGAAATGGA CGACGATGAT GATGATTACG ATCCAATAGC AGATGCGCAT 
GCGAATGGTG GTAAATGGAA ACCACCAACA ATGCGTCACT CACCGCCCGT AGTGCCCGAA 
ACGCATACTG GTGGCAAATG GCGACCCGAA TTTAGACCAG AATTGAATGT TACACACGCA 
CAACTCACAC GCATTTCGCC GAACTGGGAT GAAGCTGTCG AAGAGATGGA AAACGTGGAA 
GTGCACGAAG GCCAACATCC GCCTGCCGAA CACACGAAAG GTAAATGGAT ACCCGGTACG 
AAGTTGCAGC GACTCGAATA TAAAGAGGAA GTGGTGGAAT TGGCGAAATC GACGAATCAG 
GCGTACTGGT GTAATATCTG TTGTCGACGT TTGAAGAGCA AAACAAATTA TGAGAACCAT 
CTAAAGACAG GTTATCATCG CAAACGTGCG GAACCCGAAC GGCAGCTCGA AAAGGCGGCG 
CTGGGCGATA TACAACGTGT TGAATTGAGT AAAAGTTTAT TGGCTGCACT TGAGGAACCC 
AAACCAGCGA CCAGCAGTAG TGTTGTTGTA AAGAAGCGTA AGCGACGCGC CACTTTCATT 
AAATGTCAAC TCTGCAAGCA CAGCATGTTG CGCCATCTAG TGGGTAAGCA TTTGATATCG 
CACTATCACT ATCGACGCAT GCAGCTGAAT CCCGCGAAGT CACTGCACAT GATACTAGAC 
AATATACACT CGGTGGTACT GCAGTCACCG TTTCAATGTC GCCCATGTCG CTTCTACACC 
AACACCGAAG AGATGTTCCT GCAGCACTGG AGCTCGCCCA CACACTTGGA CGCCACCGAG 
GGACTCGGCA AGTTTTGGTG TAGTTATTGT CACTTCGAAT GCGAGGACAA CAATCAAATG 
CGTCGCCATC TGCTCAGCGC CGAACACAAG GAAGTCATAT TGGCCATTAA TCGTTCCGTA 
CCCATTTGCA TTAGCAAAAA GCTGAGCATC GACTGCCAGC GTTGTCGTCA GAGTTTCCGC 
TATAATGCCG AGTTACGTCG CCACGCCTTG TCTTGCCATC CCGGCAAGGA ATTGTGCGGC 
ACCGCATCGG ACGGCTATCA GTGTCGTTAC AAGTGTGAAT TATGCGAGGA CAGTTTTCGC 
ACGAAGGTCG CACTGCAACG GCACGAGAAG AACAAACATA GCGTGAGTAA ATATTTCTGT 
TCCATATGCA AATTGGAATT CAATACGCCT TGCAAGGCGC GCAGACATCG CAAAACGTTG 
GAGCATCGGA ATTGCGCAGC GGCTGCCGAA AAGAATGCAG GTGTGCGACG TGTGGCGGCG 
GTGAAGGACG TCAAGAGTTG TGATAAATGC AATTTCGTCG CTTCTTCCGA GGTTCAAGCT 
ATGCTGCACG AGCTCTCACA TCACAAGAGC GGTATTACCA TGCATAATAC AGTTATAACC 
AAAGCTAGTA CTAACAGTAA AAACAGCGAG AAGTCGCAAA AAGTCTGTGA AAAACCCAAA 
CTCAAGTGCA AAGATTGCGA AGAACTCTTC GCCGATAAGG AGGCACTCAA AACGCATCGG 
GACTCCAAAC ACCCACTATT GTGTCACGTC TGTTTGAGTT GTGGTCAAAG TTTTGCGTTG 
GCACAAGCGC TCGGCCGTCA TACACGCAAC TGTCAACCGA AGCCGAGTAC CTCAAAGGAA 
GCGCAGGCTA CTCGGCGTGA TGCGGAAAAT AGCTGGTGCT GCGACAAGTG TGACTTTAGC 
GCTCCTTACG AATCGGAACT AATCTTCCAT CGTCTGCATC ACACCCTTGG TCCTATGGGT 
AAAAATCAAG TTATCGCTTG TCCAATATGT ACGAAAGAGT TTCGTAAGCA TTCGTTACGT 
TGTCATCTGC GACAACACAC GAATGAGAAG ATTTTCGCTT GTGAATTCTG TGAAATGAAA 
TTTTCACGTC GTCACAATCT CAAGGATCAC ATGAAGACTG TACATAATGT GTCAGCAGCA 
GCAACAACAG GTCCATCAGC AGACATGGAT GCCCAAAAAG CGAAGAAAAG AAAAGTTGAT 
GGTGACAAAT TTGCTTGTGG TGTTTGCTCC AAGGCATTTA GCTCAGATTA TATGCTGAAA 
AAGCATAGTG CGAGCCACGC CACTTTGCCG TCGGAAAATG CTGAAAATAC TTGTAAATAT 
GAGAATTGCA AATATGTTGC CGCAAATGCC ACTTTACTGC GCGTTCATGC GGCATCACAT 
GCCAAGGAGC AGCTAAAATG CACTGAGCCA TCATGCAACT ATGAAGGGAA AAGTTTGCTG 
CATTTAAAGA GACACTCCCG CACCCACCTC GCGCCATCCA AATGGTTTAC ATGTGACCAA 
TGCGAATTCA AGGCGCGCAT CAAGGGACAT CTAAAACGTC ATCTGCGCAC TCACACTGGT 
GAAAAGCCGT TCCACTGTCG ACACTGTGAG TTCCGCTGCA GTACTTTCGA TAATTTGCGT 
AAACATGTCC TCAAAACCGG CAAACATCCA GGCAAATTCA TATACGAATG TGAGCATTGC 
AATAATGCTG AAGAGAAGAA ATTCTTCAAA ACCAATTCAT ATCACGAGTT TCAAGCGCAC 
AACACAGAGA TGCATAGGAA GGGAGTTTTG GGTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176704.2
CDS	262..3480
Translation	MIEPDLDDTHLCIKCSTTIVGLDAYIEHRKTQCTNLNSTAASSKREHTTTHTVHTEIITTPRAHLDHTYDAFAFAEPESYAKESAHGGKTSKSLSDAYELPYELGADVFFSSLQLQSVQAAGPSTSGKQGLSAPSQPLSSLQQRHNKTEHNVHIGAADEPWMSDAHAGGSNVEQQQKSYAVFKPITFEHESPEASEEEEEDVDVEMDDDDDDYDPIADAHANGGKWKPPTMRHSPPVVPETHTGGKWRPEFRPELNVTHAQLTRISPNWDEAVEEMENVEVHEGQHPPAEHTKGKWIPGTKLQRLEYKEEVVELAKSTNQAYWCNICCRRLKSKTNYENHLKTGYHRKRAEPERQLEKAALGDIQRVELSKSLLAALEEPKPATSSSVVVKKRKRRATFIKCQLCKHSMLRHLVGKHLISHYHYRRMQLNPAKSLHMILDNIHSVVLQSPFQCRPCRFYTNTEEMFLQHWSSPTHLDATEGLGKFWCSYCHFECEDNNQMRRHLLSAEHKEVILAINRSVPICISKKLSIDCQRCRQSFRYNAELRRHALSCHPGKELCGTASDGYQCRYKCELCEDSFRTKVALQRHEKNKHSVSKYFCSICKLEFNTPCKARRHRKTLEHRNCAAAAEKNAGVRRVAAVKDVKSCDKCNFVASSEVQAMLHELSHHKSGITMHNTVITKASTNSKNSEKSQKVCEKPKLKCKDCEELFADKEALKTHRDSKHPLLCHVCLSCGQSFALAQALGRHTRNCQPKPSTSKEAQATRRDAENSWCCDKCDFSAPYESELIFHRLHHTLGPMGKNQVIACPICTKEFRKHSLRCHLRQHTNEKIFACEFCEMKFSRRHNLKDHMKTVHNVSAAATTGPSADMDAQKAKKRKVDGDKFACGVCSKAFSSDYMLKKHSASHATLPSENAENTCKYENCKYVAANATLLRVHAASHAKEQLKCTEPSCNYEGKSLLHLKRHSRTHLAPSKWFTCDQCEFKARIKGHLKRHLRTHTGEKPFHCRHCEFRCSTFDNLRKHVLKTGKHPGKFIYECEHCNNAEEKKFFKTNSYHEFQAHNTEMHRKGVLGT

Target ORF information:

RefSeq Version	XM_011178402.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae zinc finger protein 26 (LOC105208515), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178402.2

ORF Insert Sequence:

ATGATCGAAC CCGATTTGGA CGACACACAT CTCTGCATCA AATGCAGCAC TACAATTGTG 
GGACTAGACG CTTACATCGA GCACCGTAAA ACGCAATGCA CCAACCTCAA TAGTACAGCT 
GCAAGTAGTA AGCGAGAGCA CACGACTACG CATACCGTGC ACACGGAAAT TATAACCACG 
CCACGTGCAC ATCTCGATCA CACCTATGAT GCTTTCGCCT TTGCCGAACC GGAGTCATAC 
GCCAAGGAGT CGGCACATGG GGGTAAAACC TCGAAATCGC TGTCCGACGC ATACGAGCTG 
CCATACGAGC TAGGGGCGGA TGTGTTCTTC TCATCACTGC AGCTGCAAAG TGTGCAAGCG 
GCCGGACCCT CAACGAGTGG TAAGCAGGGA CTGAGCGCAC CGAGTCAACC GCTGAGCAGT 
CTGCAACAGC GACACAACAA AACCGAGCAT AACGTACATA TCGGTGCCGC AGATGAGCCG 
TGGATGTCAG ATGCACATGC AGGTGGTAGT AATGTAGAGC AGCAGCAAAA ATCTTACGCA 
GTTTTCAAAC CCATCACATT CGAGCATGAA TCACCCGAGG CGAGTGAAGA AGAAGAAGAG 
GATGTTGATG TCGAAATGGA CGACGATGAT GATGATTACG ATCCAATAGC AGATGCGCAT 
GCGAATGGTG GTAAATGGAA ACCACCAACA ATGCGTCACT CACCGCCCGT AGTGCCCGAA 
ACGCATACTG GTGGCAAATG GCGACCCGAA TTTAGACCAG AATTGAATGT TACACACGCA 
CAACTCACAC GCATTTCGCC GAACTGGGAT GAAGCTGTCG AAGAGATGGA AAACGTGGAA 
GTGCACGAAG GCCAACATCC GCCTGCCGAA CACACGAAAG GTAAATGGAT ACCCGGTACG 
AAGTTGCAGC GACTCGAATA TAAAGAGGAA GTGGTGGAAT TGGCGAAATC GACGAATCAG 
GCGTACTGGT GTAATATCTG TTGTCGACGT TTGAAGAGCA AAACAAATTA TGAGAACCAT 
CTAAAGACAG GTTATCATCG CAAACGTGCG GAACCCGAAC GGCAGCTCGA AAAGGCGGCG 
CTGGGCGATA TACAACGTGT TGAATTGAGT AAAAGTTTAT TGGCTGCACT TGAGGAACCC 
AAACCAGCGA CCAGCAGTAG TGTTGTTGTA AAGAAGCGTA AGCGACGCGC CACTTTCATT 
AAATGTCAAC TCTGCAAGCA CAGCATGTTG CGCCATCTAG TGGGTAAGCA TTTGATATCG 
CACTATCACT ATCGACGCAT GCAGCTGAAT CCCGCGAAGT CACTGCACAT GATACTAGAC 
AATATACACT CGGTGGTACT GCAGTCACCG TTTCAATGTC GCCCATGTCG CTTCTACACC 
AACACCGAAG AGATGTTCCT GCAGCACTGG AGCTCGCCCA CACACTTGGA CGCCACCGAG 
GGACTCGGCA AGTTTTGGTG TAGTTATTGT CACTTCGAAT GCGAGGACAA CAATCAAATG 
CGTCGCCATC TGCTCAGCGC CGAACACAAG GAAGTCATAT TGGCCATTAA TCGTTCCGTA 
CCCATTTGCA TTAGCAAAAA GCTGAGCATC GACTGCCAGC GTTGTCGTCA GAGTTTCCGC 
TATAATGCCG AGTTACGTCG CCACGCCTTG TCTTGCCATC CCGGCAAGGA ATTGTGCGGC 
ACCGCATCGG ACGGCTATCA GTGTCGTTAC AAGTGTGAAT TATGCGAGGA CAGTTTTCGC 
ACGAAGGTCG CACTGCAACG GCACGAGAAG AACAAACATA GCGTGAGTAA ATATTTCTGT 
TCCATATGCA AATTGGAATT CAATACGCCT TGCAAGGCGC GCAGACATCG CAAAACGTTG 
GAGCATCGGA ATTGCGCAGC GGCTGCCGAA AAGAATGCAG GTGTGCGACG TGTGGCGGCG 
GTGAAGGACG TCAAGAGTTG TGATAAATGC AATTTCGTCG CTTCTTCCGA GGTTCAAGCT 
ATGCTGCACG AGCTCTCACA TCACAAGAGC GGTATTACCA TGCATAATAC AGTTATAACC 
AAAGCTAGTA CTAACAGTAA AAACAGCGAG AAGTCGCAAA AAGTCTGTGA AAAACCCAAA 
CTCAAGTGCA AAGATTGCGA AGAACTCTTC GCCGATAAGG AGGCACTCAA AACGCATCGG 
GACTCCAAAC ACCCACTATT GTGTCACGTC TGTTTGAGTT GTGGTCAAAG TTTTGCGTTG 
GCACAAGCGC TCGGCCGTCA TACACGCAAC TGTCAACCGA AGCCGAGTAC CTCAAAGGAA 
GCGCAGGCTA CTCGGCGTGA TGCGGAAAAT AGCTGGTGCT GCGACAAGTG TGACTTTAGC 
GCTCCTTACG AATCGGAACT AATCTTCCAT CGTCTGCATC ACACCCTTGG TCCTATGGGT 
AAAAATCAAG TTATCGCTTG TCCAATATGT ACGAAAGAGT TTCGTAAGCA TTCGTTACGT 
TGTCATCTGC GACAACACAC GAATGAGAAG ATTTTCGCTT GTGAATTCTG TGAAATGAAA 
TTTTCACGTC GTCACAATCT CAAGGATCAC ATGAAGACTG TACATAATGT GTCAGCAGCA 
GCAACAACAG GTCCATCAGC AGACATGGAT GCCCAAAAAG CGAAGAAAAG AAAAGTTGAT 
GGTGACAAAT TTGCTTGTGG TGTTTGCTCC AAGGCATTTA GCTCAGATTA TATGCTGAAA 
AAGCATAGTG CGAGCCACGC CACTTTGCCG TCGGAAAATG CTGAAAATAC TTGTAAATAT 
GAGAATTGCA AATATGTTGC CGCAAATGCC ACTTTACTGC GCGTTCATGC GGCATCACAT 
GCCAAGGAGC AGCTAAAATG CACTGAGCCA TCATGCAACT ATGAAGGGAA AAGTTTGCTG 
CATTTAAAGA GACACTCCCG CACCCACCTC GCGCCATCCA AATGGTTTAC ATGTGACCAA 
TGCGAATTCA AGGCGCGCAT CAAGGGACAT CTAAAACGTC ATCTGCGCAC TCACACTGGT 
GAAAAGCCGT TCCACTGTCG ACACTGTGAG TTCCGCTGCA GTACTTTCGA TAATTTGCGT 
AAACATGTCC TCAAAACCGG CAAACATCCA GGCAAATTCA TATACGAATG TGAGCATTGC 
AATAATGCTG AAGAGAAGAA ATTCTTCAAA ACCAATTCAT ATCACGAGTT TCAAGCGCAC 
AACACAGAGA TGCATAGGAA GGGAGTTTTG GGTACATAA