LOC105208370 gene cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

mRNA	Protein	Name
XM_011178137.1	XP_011176439.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178154.1	XP_011176456.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178143.1	XP_011176445.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178160.1	XP_011176462.1	GATA zinc finger domain-containing protein 11 isoform X2
XM_029037804.1	XP_028893637.1	putative uncharacterized protein DDB_G0282133 isoform X1
XM_011178138.1	XP_011176440.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178140.1	XP_011176442.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178141.1	XP_011176443.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178145.1	XP_011176447.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178149.1	XP_011176451.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178152.1	XP_011176454.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178157.1	XP_011176459.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178159.1	XP_011176461.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_029037802.1	XP_028893635.1	putative uncharacterized protein DDB_G0282133 isoform X1
XM_029037806.1	XP_028893639.1	GATA zinc finger domain-containing protein 11 isoform X3
XM_011178148.1	XP_011176450.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178150.1	XP_011176452.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178153.1	XP_011176455.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_029037803.1	XP_028893636.1	putative uncharacterized protein DDB_G0282133 isoform X1
XM_011178158.2	XP_011176460.1	putative uncharacterized protein DDB_G0282133 isoform X1
XM_029037805.1	XP_028893638.1	ras guanine nucleotide exchange factor P isoform X2
XM_011178139.1	XP_011176441.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178142.1	XP_011176444.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178146.1	XP_011176448.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178147.1	XP_011176449.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178151.1	XP_011176453.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178156.1	XP_011176458.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_011178158.1	XP_011176460.1	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
XM_029037801.1	XP_028893634.1	putative uncharacterized protein DDB_G0282133 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa194035	XM_029037806.1 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa194035	XM_011178160.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa254438	XM_029037805.1 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa194034	XM_011178156.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178153.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178159.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178150.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_029037803.1 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178141.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178139.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_029037802.1 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178142.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178147.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_029037804.1 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178138.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178140.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178143.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178146.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_029037801.1 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178137.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178158.2 Latest version!	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178149.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178151.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178148.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178145.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178154.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178152.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa194034	XM_011178157.1 Latest version!	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OBa194034	XM_011178158.1	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

LOC105208370 ( XM_011178160.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176462.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OBa194035
Clone ID Related Accession (Same CDS sequence)	XM_011178160.1 , XM_029037806.1
Accession Version	XM_011178160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	GATA zinc finger domain-containing protein 11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGTAGCAACA ACAACAACGT CAACAATAAT 
AACCATCATC AGCAATTCAA TAATTTGAGT ATGGGACAGC AATCTCATCT ACGGCATATA 
CGTTTTAGCA ATAACGGGCG TGGCATGGAT AATGGCAGTA ACCATAATGA ATATAATATT 
AGTAACAACA ACAATTACCG ACGAGGGGGA AACCAAAGTA ACAACAGTAG TGGCAATAGT 
ATCCCGATGA TGATCCATCG TCACAATAAT GAATATTATG GGAATAATCA TCACAATTAT 
CAACACCATC AATATAATGA ACATAGTCGT CGACACAATG TCATTAACGG CAACAACAAT 
AATAACAATA GCAATAGCAG TAATGCGTCT AACTCAGGAT TATTTGATAG AAACCATAAT 
AATAATAATA GTACCAATAA CAATGTAAAT GTAAATGTGA ACGGGAACGG GAACAATGGC 
TGCAACAACA ACAGTGTGCA TTTTGATGGA CTTAGTGGCA ACAACCATTC GCGGAGTGTT 
GATTTCCACA CTGACAATCG CACAGAACGT GGCAGTATAG CCGAGGCTAC TAATAATGGC 
AGTGGCGGTA ACTTTAGCTA CATCTCGGGA CGAAATAATA GCAATTATGG GCGTAACGGT 
CACCAAAATA ACAGTTCTGG TAATTACGGC TACCATCATC ATCATCACTA TAGCAATAAT 
GGGCCATCAA CATCAGGATT ATCAGGCTTT AGCGGTAGTG TCTCCAACCT TTTTGATGGA 
CCAAGTGGCT CTTCTGGTAT TATGGGATCC TCTTCTTCAC TAAATAGTGG CCCCCATGGG 
TTGAAATCCG ACAGTCCATC GCGAAAGAGG CGTCGCATCT CTGGCCGCAT GCCCAGCCAG 
TCACCGCCAG CGATATGGGA GCAAAGACGT TCTCCTCGCA ATCAAATGCA GCAGGGAAGT 
CCTCCGATAC GCCGCCCCCG TCTGCATGAT GCTGGGTCAT CTCATCATAA CCGCGGCATT 
CATGGTAGCA ACAATAGTGT TTCGGGTAAT AGTATCAACA ACACGAATAC AAATAACGTT 
AGTGGAAACC ATTTGCAAAG TTATCACCAA CCACAGCATT CAATACAATC CACGCCATAT 
TACCTCCGGC AGCACATGCC ACCTCCACCC GCTCTACAAC AACAACAAAC ACCATCGCCG 
CCAACGCATC ATCAACCACA GTCCTTACCA CAGCCACAAT TACAGCAACG TTCACCTTGG 
GAGCACTCAT TATCTGCTGC TGCAGTTCTA ACTAGCTCGA ACCAAGCGCC TGCAGCCGTG 
AACGCTTATA TGCAGCAAAC TCCACCACCA CCACAGTCCC CTGGTCATCA TCACCAAACA 
TCCTTGGTAG GAGCACCACC GCCACCCCCA CCTTTGATGT TGGACATCAA TCAGGTTCCT 
GTCAGTCTTC CTTTACGTCA CGCCGAGCCT ATATGGGCCT CTATATGCAC TTATCCAGCG 
CCACCGCCTC AAGCAAGAAT AGCACCTTGT CATCTGCATG GTATTTATAC ACAACCGTTC 
GCATCTCAAG CATGCAATAC ACATCCCAGT GCTCAATTCA CGCAAGCCAC GACTAGTTTC 
GCTGCTACCG CCGGTACACC AATTCAAATG CCACAACCAC AACAGGTCCA GGTTAATGCA 
GCTGCAGCCG CACAACAACA ACACACACAA CAAGCTGCCG CCCTTATGGC AGCTGCTTCG 
GTTGCCGCGG CTAACTACCA ACATGCTCAA TTTACCCAAC AACAACGTAC GGAGGCTACT 
GCAGCCGCTG CGGCTGCTGC TGCTGCCGTA GCTGGTTTAT CTCTAGAACA AGCCGGCGCT 
CATCACCTAG ACCCCCATCA AGGACATCAA GCTGCTGTAC CGACTCCAAC GGCACATCAC 
AATCAATTAC AAGCCACACC AATTCATATA ACCTCCATGT CAGCAGTTGC GGCTGCAGCA 
GCACACCATC TGCGTACTAC TGCAACAGCA GCGGCTCAAA TGTCTGCTGC ACCTCAACCG 
ATCTTGCTCT CTGCAGAGAG GCGTGTTTTT CCACCACATC GTCGTATTAC CCGTTTTTGG 
CCAGCAAGTC ATCCACACGG ACCTCACCGA CATATGCTTT CACCACAAAC TTTGGGACCA 
CACCAAGCGA CACCTGTACA AATTCAGACG ACAACTGGAA TCATTAATCC GGGCTTTTTA 
CTTAATTTCC TTGCCATGTT CCCGCTCTCA CCTTATAATC AACATGATCT AAACTCACTA 
GATTCAAACG AAACGGAAAA TTACGAAGCT TTGTTAAGTT TGGCGGAACG TTTGGGTGAG 
GCTAAGCCAC GCGGATTGGC GCGAAATGAA ATCGATCAGC TGCCAAGCTA TAAGTATAAT 
CCAGATACCC ATAGTGGTGA TCAAACGTCT TGCGTTGTTT GCATGTGTGA CTTTGAGTTA 
AAGCAAGTAC TCCGAGTGCT ACCTTGTTCA CATGAATTTC ATGCAAAATG TGTGGATAAA 
TGGCTAAGGT CAAATCGAAC CTGCCCCATT TGTCGGGGTA ATGCTTCGGA CTACTTTGAA 
AATCCAGAGC AGCATCAACA GCAACAGCAG CAAACTCAAC AGACACAGTC TCAAATCCAA 
CCACAATCTT CATCGCAACA ATCTCAAACG CAGCCAGCAG CTACAACACA AAATGCGATT 
TCAGGAGCGG CACAGCCGAC AGCCCAACAA CAAACACAAG CAACCCATGC ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176462.1
CDS	1644..4640
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178160.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178160.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGTAGCAACA ACAACAACGT CAACAATAAT 
AACCATCATC AGCAATTCAA TAATTTGAGT ATGGGACAGC AATCTCATCT ACGGCATATA 
CGTTTTAGCA ATAACGGGCG TGGCATGGAT AATGGCAGTA ACCATAATGA ATATAATATT 
AGTAACAACA ACAATTACCG ACGAGGGGGA AACCAAAGTA ACAACAGTAG TGGCAATAGT 
ATCCCGATGA TGATCCATCG TCACAATAAT GAATATTATG GGAATAATCA TCACAATTAT 
CAACACCATC AATATAATGA ACATAGTCGT CGACACAATG TCATTAACGG CAACAACAAT 
AATAACAATA GCAATAGCAG TAATGCGTCT AACTCAGGAT TATTTGATAG AAACCATAAT 
AATAATAATA GTACCAATAA CAATGTAAAT GTAAATGTGA ACGGGAACGG GAACAATGGC 
TGCAACAACA ACAGTGTGCA TTTTGATGGA CTTAGTGGCA ACAACCATTC GCGGAGTGTT 
GATTTCCACA CTGACAATCG CACAGAACGT GGCAGTATAG CCGAGGCTAC TAATAATGGC 
AGTGGCGGTA ACTTTAGCTA CATCTCGGGA CGAAATAATA GCAATTATGG GCGTAACGGT 
CACCAAAATA ACAGTTCTGG TAATTACGGC TACCATCATC ATCATCACTA TAGCAATAAT 
GGGCCATCAA CATCAGGATT ATCAGGCTTT AGCGGTAGTG TCTCCAACCT TTTTGATGGA 
CCAAGTGGCT CTTCTGGTAT TATGGGATCC TCTTCTTCAC TAAATAGTGG CCCCCATGGG 
TTGAAATCCG ACAGTCCATC GCGAAAGAGG CGTCGCATCT CTGGCCGCAT GCCCAGCCAG 
TCACCGCCAG CGATATGGGA GCAAAGACGT TCTCCTCGCA ATCAAATGCA GCAGGGAAGT 
CCTCCGATAC GCCGCCCCCG TCTGCATGAT GCTGGGTCAT CTCATCATAA CCGCGGCATT 
CATGGTAGCA ACAATAGTGT TTCGGGTAAT AGTATCAACA ACACGAATAC AAATAACGTT 
AGTGGAAACC ATTTGCAAAG TTATCACCAA CCACAGCATT CAATACAATC CACGCCATAT 
TACCTCCGGC AGCACATGCC ACCTCCACCC GCTCTACAAC AACAACAAAC ACCATCGCCG 
CCAACGCATC ATCAACCACA GTCCTTACCA CAGCCACAAT TACAGCAACG TTCACCTTGG 
GAGCACTCAT TATCTGCTGC TGCAGTTCTA ACTAGCTCGA ACCAAGCGCC TGCAGCCGTG 
AACGCTTATA TGCAGCAAAC TCCACCACCA CCACAGTCCC CTGGTCATCA TCACCAAACA 
TCCTTGGTAG GAGCACCACC GCCACCCCCA CCTTTGATGT TGGACATCAA TCAGGTTCCT 
GTCAGTCTTC CTTTACGTCA CGCCGAGCCT ATATGGGCCT CTATATGCAC TTATCCAGCG 
CCACCGCCTC AAGCAAGAAT AGCACCTTGT CATCTGCATG GTATTTATAC ACAACCGTTC 
GCATCTCAAG CATGCAATAC ACATCCCAGT GCTCAATTCA CGCAAGCCAC GACTAGTTTC 
GCTGCTACCG CCGGTACACC AATTCAAATG CCACAACCAC AACAGGTCCA GGTTAATGCA 
GCTGCAGCCG CACAACAACA ACACACACAA CAAGCTGCCG CCCTTATGGC AGCTGCTTCG 
GTTGCCGCGG CTAACTACCA ACATGCTCAA TTTACCCAAC AACAACGTAC GGAGGCTACT 
GCAGCCGCTG CGGCTGCTGC TGCTGCCGTA GCTGGTTTAT CTCTAGAACA AGCCGGCGCT 
CATCACCTAG ACCCCCATCA AGGACATCAA GCTGCTGTAC CGACTCCAAC GGCACATCAC 
AATCAATTAC AAGCCACACC AATTCATATA ACCTCCATGT CAGCAGTTGC GGCTGCAGCA 
GCACACCATC TGCGTACTAC TGCAACAGCA GCGGCTCAAA TGTCTGCTGC ACCTCAACCG 
ATCTTGCTCT CTGCAGAGAG GCGTGTTTTT CCACCACATC GTCGTATTAC CCGTTTTTGG 
CCAGCAAGTC ATCCACACGG ACCTCACCGA CATATGCTTT CACCACAAAC TTTGGGACCA 
CACCAAGCGA CACCTGTACA AATTCAGACG ACAACTGGAA TCATTAATCC GGGCTTTTTA 
CTTAATTTCC TTGCCATGTT CCCGCTCTCA CCTTATAATC AACATGATCT AAACTCACTA 
GATTCAAACG AAACGGAAAA TTACGAAGCT TTGTTAAGTT TGGCGGAACG TTTGGGTGAG 
GCTAAGCCAC GCGGATTGGC GCGAAATGAA ATCGATCAGC TGCCAAGCTA TAAGTATAAT 
CCAGATACCC ATAGTGGTGA TCAAACGTCT TGCGTTGTTT GCATGTGTGA CTTTGAGTTA 
AAGCAAGTAC TCCGAGTGCT ACCTTGTTCA CATGAATTTC ATGCAAAATG TGTGGATAAA 
TGGCTAAGGT CAAATCGAAC CTGCCCCATT TGTCGGGGTA ATGCTTCGGA CTACTTTGAA 
AATCCAGAGC AGCATCAACA GCAACAGCAG CAAACTCAAC AGACACAGTC TCAAATCCAA 
CCACAATCTT CATCGCAACA ATCTCAAACG CAGCCAGCAG CTACAACACA AAATGCGATT 
TCAGGAGCGG CACAGCCGAC AGCCCAACAA CAAACACAAG CAACCCATGC ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_029037806.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893639.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa194035
Clone ID Related Accession (Same CDS sequence)	XM_011178160.1 , XM_029037806.1
Accession Version	XM_029037806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	GATA zinc finger domain-containing protein 11 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGTAGCAACA ACAACAACGT CAACAATAAT 
AACCATCATC AGCAATTCAA TAATTTGAGT ATGGGACAGC AATCTCATCT ACGGCATATA 
CGTTTTAGCA ATAACGGGCG TGGCATGGAT AATGGCAGTA ACCATAATGA ATATAATATT 
AGTAACAACA ACAATTACCG ACGAGGGGGA AACCAAAGTA ACAACAGTAG TGGCAATAGT 
ATCCCGATGA TGATCCATCG TCACAATAAT GAATATTATG GGAATAATCA TCACAATTAT 
CAACACCATC AATATAATGA ACATAGTCGT CGACACAATG TCATTAACGG CAACAACAAT 
AATAACAATA GCAATAGCAG TAATGCGTCT AACTCAGGAT TATTTGATAG AAACCATAAT 
AATAATAATA GTACCAATAA CAATGTAAAT GTAAATGTGA ACGGGAACGG GAACAATGGC 
TGCAACAACA ACAGTGTGCA TTTTGATGGA CTTAGTGGCA ACAACCATTC GCGGAGTGTT 
GATTTCCACA CTGACAATCG CACAGAACGT GGCAGTATAG CCGAGGCTAC TAATAATGGC 
AGTGGCGGTA ACTTTAGCTA CATCTCGGGA CGAAATAATA GCAATTATGG GCGTAACGGT 
CACCAAAATA ACAGTTCTGG TAATTACGGC TACCATCATC ATCATCACTA TAGCAATAAT 
GGGCCATCAA CATCAGGATT ATCAGGCTTT AGCGGTAGTG TCTCCAACCT TTTTGATGGA 
CCAAGTGGCT CTTCTGGTAT TATGGGATCC TCTTCTTCAC TAAATAGTGG CCCCCATGGG 
TTGAAATCCG ACAGTCCATC GCGAAAGAGG CGTCGCATCT CTGGCCGCAT GCCCAGCCAG 
TCACCGCCAG CGATATGGGA GCAAAGACGT TCTCCTCGCA ATCAAATGCA GCAGGGAAGT 
CCTCCGATAC GCCGCCCCCG TCTGCATGAT GCTGGGTCAT CTCATCATAA CCGCGGCATT 
CATGGTAGCA ACAATAGTGT TTCGGGTAAT AGTATCAACA ACACGAATAC AAATAACGTT 
AGTGGAAACC ATTTGCAAAG TTATCACCAA CCACAGCATT CAATACAATC CACGCCATAT 
TACCTCCGGC AGCACATGCC ACCTCCACCC GCTCTACAAC AACAACAAAC ACCATCGCCG 
CCAACGCATC ATCAACCACA GTCCTTACCA CAGCCACAAT TACAGCAACG TTCACCTTGG 
GAGCACTCAT TATCTGCTGC TGCAGTTCTA ACTAGCTCGA ACCAAGCGCC TGCAGCCGTG 
AACGCTTATA TGCAGCAAAC TCCACCACCA CCACAGTCCC CTGGTCATCA TCACCAAACA 
TCCTTGGTAG GAGCACCACC GCCACCCCCA CCTTTGATGT TGGACATCAA TCAGGTTCCT 
GTCAGTCTTC CTTTACGTCA CGCCGAGCCT ATATGGGCCT CTATATGCAC TTATCCAGCG 
CCACCGCCTC AAGCAAGAAT AGCACCTTGT CATCTGCATG GTATTTATAC ACAACCGTTC 
GCATCTCAAG CATGCAATAC ACATCCCAGT GCTCAATTCA CGCAAGCCAC GACTAGTTTC 
GCTGCTACCG CCGGTACACC AATTCAAATG CCACAACCAC AACAGGTCCA GGTTAATGCA 
GCTGCAGCCG CACAACAACA ACACACACAA CAAGCTGCCG CCCTTATGGC AGCTGCTTCG 
GTTGCCGCGG CTAACTACCA ACATGCTCAA TTTACCCAAC AACAACGTAC GGAGGCTACT 
GCAGCCGCTG CGGCTGCTGC TGCTGCCGTA GCTGGTTTAT CTCTAGAACA AGCCGGCGCT 
CATCACCTAG ACCCCCATCA AGGACATCAA GCTGCTGTAC CGACTCCAAC GGCACATCAC 
AATCAATTAC AAGCCACACC AATTCATATA ACCTCCATGT CAGCAGTTGC GGCTGCAGCA 
GCACACCATC TGCGTACTAC TGCAACAGCA GCGGCTCAAA TGTCTGCTGC ACCTCAACCG 
ATCTTGCTCT CTGCAGAGAG GCGTGTTTTT CCACCACATC GTCGTATTAC CCGTTTTTGG 
CCAGCAAGTC ATCCACACGG ACCTCACCGA CATATGCTTT CACCACAAAC TTTGGGACCA 
CACCAAGCGA CACCTGTACA AATTCAGACG ACAACTGGAA TCATTAATCC GGGCTTTTTA 
CTTAATTTCC TTGCCATGTT CCCGCTCTCA CCTTATAATC AACATGATCT AAACTCACTA 
GATTCAAACG AAACGGAAAA TTACGAAGCT TTGTTAAGTT TGGCGGAACG TTTGGGTGAG 
GCTAAGCCAC GCGGATTGGC GCGAAATGAA ATCGATCAGC TGCCAAGCTA TAAGTATAAT 
CCAGATACCC ATAGTGGTGA TCAAACGTCT TGCGTTGTTT GCATGTGTGA CTTTGAGTTA 
AAGCAAGTAC TCCGAGTGCT ACCTTGTTCA CATGAATTTC ATGCAAAATG TGTGGATAAA 
TGGCTAAGGT CAAATCGAAC CTGCCCCATT TGTCGGGGTA ATGCTTCGGA CTACTTTGAA 
AATCCAGAGC AGCATCAACA GCAACAGCAG CAAACTCAAC AGACACAGTC TCAAATCCAA 
CCACAATCTT CATCGCAACA ATCTCAAACG CAGCCAGCAG CTACAACACA AAATGCGATT 
TCAGGAGCGG CACAGCCGAC AGCCCAACAA CAAACACAAG CAACCCATGC ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893639.1
CDS	170..3166
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_029037806.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037806.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGTAGCAACA ACAACAACGT CAACAATAAT 
AACCATCATC AGCAATTCAA TAATTTGAGT ATGGGACAGC AATCTCATCT ACGGCATATA 
CGTTTTAGCA ATAACGGGCG TGGCATGGAT AATGGCAGTA ACCATAATGA ATATAATATT 
AGTAACAACA ACAATTACCG ACGAGGGGGA AACCAAAGTA ACAACAGTAG TGGCAATAGT 
ATCCCGATGA TGATCCATCG TCACAATAAT GAATATTATG GGAATAATCA TCACAATTAT 
CAACACCATC AATATAATGA ACATAGTCGT CGACACAATG TCATTAACGG CAACAACAAT 
AATAACAATA GCAATAGCAG TAATGCGTCT AACTCAGGAT TATTTGATAG AAACCATAAT 
AATAATAATA GTACCAATAA CAATGTAAAT GTAAATGTGA ACGGGAACGG GAACAATGGC 
TGCAACAACA ACAGTGTGCA TTTTGATGGA CTTAGTGGCA ACAACCATTC GCGGAGTGTT 
GATTTCCACA CTGACAATCG CACAGAACGT GGCAGTATAG CCGAGGCTAC TAATAATGGC 
AGTGGCGGTA ACTTTAGCTA CATCTCGGGA CGAAATAATA GCAATTATGG GCGTAACGGT 
CACCAAAATA ACAGTTCTGG TAATTACGGC TACCATCATC ATCATCACTA TAGCAATAAT 
GGGCCATCAA CATCAGGATT ATCAGGCTTT AGCGGTAGTG TCTCCAACCT TTTTGATGGA 
CCAAGTGGCT CTTCTGGTAT TATGGGATCC TCTTCTTCAC TAAATAGTGG CCCCCATGGG 
TTGAAATCCG ACAGTCCATC GCGAAAGAGG CGTCGCATCT CTGGCCGCAT GCCCAGCCAG 
TCACCGCCAG CGATATGGGA GCAAAGACGT TCTCCTCGCA ATCAAATGCA GCAGGGAAGT 
CCTCCGATAC GCCGCCCCCG TCTGCATGAT GCTGGGTCAT CTCATCATAA CCGCGGCATT 
CATGGTAGCA ACAATAGTGT TTCGGGTAAT AGTATCAACA ACACGAATAC AAATAACGTT 
AGTGGAAACC ATTTGCAAAG TTATCACCAA CCACAGCATT CAATACAATC CACGCCATAT 
TACCTCCGGC AGCACATGCC ACCTCCACCC GCTCTACAAC AACAACAAAC ACCATCGCCG 
CCAACGCATC ATCAACCACA GTCCTTACCA CAGCCACAAT TACAGCAACG TTCACCTTGG 
GAGCACTCAT TATCTGCTGC TGCAGTTCTA ACTAGCTCGA ACCAAGCGCC TGCAGCCGTG 
AACGCTTATA TGCAGCAAAC TCCACCACCA CCACAGTCCC CTGGTCATCA TCACCAAACA 
TCCTTGGTAG GAGCACCACC GCCACCCCCA CCTTTGATGT TGGACATCAA TCAGGTTCCT 
GTCAGTCTTC CTTTACGTCA CGCCGAGCCT ATATGGGCCT CTATATGCAC TTATCCAGCG 
CCACCGCCTC AAGCAAGAAT AGCACCTTGT CATCTGCATG GTATTTATAC ACAACCGTTC 
GCATCTCAAG CATGCAATAC ACATCCCAGT GCTCAATTCA CGCAAGCCAC GACTAGTTTC 
GCTGCTACCG CCGGTACACC AATTCAAATG CCACAACCAC AACAGGTCCA GGTTAATGCA 
GCTGCAGCCG CACAACAACA ACACACACAA CAAGCTGCCG CCCTTATGGC AGCTGCTTCG 
GTTGCCGCGG CTAACTACCA ACATGCTCAA TTTACCCAAC AACAACGTAC GGAGGCTACT 
GCAGCCGCTG CGGCTGCTGC TGCTGCCGTA GCTGGTTTAT CTCTAGAACA AGCCGGCGCT 
CATCACCTAG ACCCCCATCA AGGACATCAA GCTGCTGTAC CGACTCCAAC GGCACATCAC 
AATCAATTAC AAGCCACACC AATTCATATA ACCTCCATGT CAGCAGTTGC GGCTGCAGCA 
GCACACCATC TGCGTACTAC TGCAACAGCA GCGGCTCAAA TGTCTGCTGC ACCTCAACCG 
ATCTTGCTCT CTGCAGAGAG GCGTGTTTTT CCACCACATC GTCGTATTAC CCGTTTTTGG 
CCAGCAAGTC ATCCACACGG ACCTCACCGA CATATGCTTT CACCACAAAC TTTGGGACCA 
CACCAAGCGA CACCTGTACA AATTCAGACG ACAACTGGAA TCATTAATCC GGGCTTTTTA 
CTTAATTTCC TTGCCATGTT CCCGCTCTCA CCTTATAATC AACATGATCT AAACTCACTA 
GATTCAAACG AAACGGAAAA TTACGAAGCT TTGTTAAGTT TGGCGGAACG TTTGGGTGAG 
GCTAAGCCAC GCGGATTGGC GCGAAATGAA ATCGATCAGC TGCCAAGCTA TAAGTATAAT 
CCAGATACCC ATAGTGGTGA TCAAACGTCT TGCGTTGTTT GCATGTGTGA CTTTGAGTTA 
AAGCAAGTAC TCCGAGTGCT ACCTTGTTCA CATGAATTTC ATGCAAAATG TGTGGATAAA 
TGGCTAAGGT CAAATCGAAC CTGCCCCATT TGTCGGGGTA ATGCTTCGGA CTACTTTGAA 
AATCCAGAGC AGCATCAACA GCAACAGCAG CAAACTCAAC AGACACAGTC TCAAATCCAA 
CCACAATCTT CATCGCAACA ATCTCAAACG CAGCCAGCAG CTACAACACA AAATGCGATT 
TCAGGAGCGG CACAGCCGAC AGCCCAACAA CAAACACAAG CAACCCATGC ACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_029037805.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893638.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa254438
Clone ID Related Accession (Same CDS sequence)	XM_029037805.1
Accession Version	XM_029037805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	ras guanine nucleotide exchange factor P isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCACC AACTTTTGAA CAACCACCAC AAAAAATTGC GCCGAACAAA ATTTCAATCC 
GAACATCGTA TGTTAATAAA AATTACAAAA ATGACAATCA CATCTGTATC ATTTTCAAAA 
CATCAATCAC AACAACGGCT TCTATCGCAA CATCCACGCA CTGTGACTGC ATCTCTAATG 
TCTTCTCGAT ATTTATCGAC AATTACCACA CCAATGTCAC CTACAATGGT ATCTAAACGA 
TTAATATGTA ATGAAAAAAA TAAAATAACA TCAATAATGA ATGCACTTGT CATAATGATG 
ACAACAACAA TGCTCCCACC ATCTATGGGG ATGGCAACAA TATCTGCAAC TTCAACATAT 
TCAACGATAA CTTCAACACC GTCAATTATT CAAAAACCAC GAAACCTCGA TCTCATTCGA 
AACCCACCCT TTTCCGATTT CATTTCTACG CTTTCTAATT GTAATCATTC CACATCTATT 
TCTACCCGAA TTTCAACTTT TGTGCCCGTT TTTTCCAACG ATTTTTTCAC ATCTATTTCT 
GAAATGTCTA CGATCTCATT GGGAACATCG TATGCTCTTG ATTTTGCATC TTCCTCCATC 
TTGACGTCGG CGCGCGTTAT CAAACTGAAT TCAACAAATA ACGAAAACAA TAACAATCAT 
AATCACATTC TAAATAAACA AAATTACAAT AATAATAATA AAACACATTT AAAATACTCA 
AACGATTGCG ATTCGACTTG TGGTACCGCC AATGGTAATA ATATTAATAA CAACAGTAGC 
AACAACAACA ACGTCAACAA TAATAACCAT CATCAGCAAT TCAATAATTT GAGTATGGGA 
CAGCAATCTC ATCTACGGCA TATACGTTTT AGCAATAACG GGCGTGGCAT GGATAATGGC 
AGTAACCATA ATGAATATAA TATTAGTAAC AACAACAATT ACCGACGAGG GGGAAACCAA 
AGTAACAACA GTAGTGGCAA TAGTATCCCG ATGATGATCC ATCGTCACAA TAATGAATAT 
TATGGGAATA ATCATCACAA TTATCAACAC CATCAATATA ATGAACATAG TCGTCGACAC 
AATGTCATTA ACGGCAACAA CAATAATAAC AATAGCAATA GCAGTAATGC GTCTAACTCA 
GGATTATTTG ATAGAAACCA TAATAATAAT AATAGTACCA ATAACAATGT AAATGTAAAT 
GTGAACGGGA ACGGGAACAA TGGCTGCAAC AACAACAGTG TGCATTTTGA TGGACTTAGT 
GGCAACAACC ATTCGCGGAG TGTTGATTTC CACACTGACA ATCGCACAGA ACGTGGCAGT 
ATAGCCGAGG CTACTAATAA TGGCAGTGGC GGTAACTTTA GCTACATCTC GGGACGAAAT 
AATAGCAATT ATGGGCGTAA CGGTCACCAA AATAACAGTT CTGGTAATTA CGGCTACCAT 
CATCATCATC ACTATAGCAA TAATGGGCCA TCAACATCAG GATTATCAGG CTTTAGCGGT 
AGTGTCTCCA ACCTTTTTGA TGGACCAAGT GGCTCTTCTG GTATTATGGG ATCCTCTTCT 
TCACTAAATA GTGGCCCCCA TGGGTTGAAA TCCGACAGTC CATCGCGAAA GAGGCGTCGC 
ATCTCTGGCC GCATGCCCAG CCAGTCACCG CCAGCGATAT GGGAGCAAAG ACGTTCTCCT 
CGCAATCAAA TGCAGCAGGG AAGTCCTCCG ATACGCCGCC CCCGTCTGCA TGATGCTGGG 
TCATCTCATC ATAACCGCGG CATTCATGGT AGCAACAATA GTGTTTCGGG TAATAGTATC 
AACAACACGA ATACAAATAA CGTTAGTGGA AACCATTTGC AAAGTTATCA CCAACCACAG 
CATTCAATAC AATCCACGCC ATATTACCTC CGGCAGCACA TGCCACCTCC ACCCGCTCTA 
CAACAACAAC AAACACCATC GCCGCCAACG CATCATCAAC CACAGTCCTT ACCACAGCCA 
CAATTACAGC AACGTTCACC TTGGGAGCAC TCATTATCTG CTGCTGCAGT TCTAACTAGC 
TCGAACCAAG CGCCTGCAGC CGTGAACGCT TATATGCAGC AAACTCCACC ACCACCACAG 
TCCCCTGGTC ATCATCACCA AACATCCTTG GTAGGAGCAC CACCGCCACC CCCACCTTTG 
ATGTTGGACA TCAATCAGGT TCCTGTCAGT CTTCCTTTAC GTCACGCCGA GCCTATATGG 
GCCTCTATAT GCACTTATCC AGCGCCACCG CCTCAAGCAA GAATAGCACC TTGTCATCTG 
CATGGTATTT ATACACAACC GTTCGCATCT CAAGCATGCA ATACACATCC CAGTGCTCAA 
TTCACGCAAG CCACGACTAG TTTCGCTGCT ACCGCCGGTA CACCAATTCA AATGCCACAA 
CCACAACAGG TCCAGGTTAA TGCAGCTGCA GCCGCACAAC AACAACACAC ACAACAAGCT 
GCCGCCCTTA TGGCAGCTGC TTCGGTTGCC GCGGCTAACT ACCAACATGC TCAATTTACC 
CAACAACAAC GTACGGAGGC TACTGCAGCC GCTGCGGCTG CTGCTGCTGC CGTAGCTGGT 
TTATCTCTAG AACAAGCCGG CGCTCATCAC CTAGACCCCC ATCAAGGACA TCAAGCTGCT 
GTACCGACTC CAACGGCACA TCACAATCAA TTACAAGCCA CACCAATTCA TATAACCTCC 
ATGTCAGCAG TTGCGGCTGC AGCAGCACAC CATCTGCGTA CTACTGCAAC AGCAGCGGCT 
CAAATGTCTG CTGCACCTCA ACCGATCTTG CTCTCTGCAG AGAGGCGTGT TTTTCCACCA 
CATCGTCGTA TTACCCGTTT TTGGCCAGCA AGTCATCCAC ACGGACCTCA CCGACATATG 
CTTTCACCAC AAACTTTGGG ACCACACCAA GCGACACCTG TACAAATTCA GACGACAACT 
GGAATCATTA ATCCGGGCTT TTTACTTAAT TTCCTTGCCA TGTTCCCGCT CTCACCTTAT 
AATCAACATG ATCTAAACTC ACTAGATTCA AACGAAACGG AAAATTACGA AGCTTTGTTA 
AGTTTGGCGG AACGTTTGGG TGAGGCTAAG CCACGCGGAT TGGCGCGAAA TGAAATCGAT 
CAGCTGCCAA GCTATAAGTA TAATCCAGAT ACCCATAGTG GTGATCAAAC GTCTTGCGTT 
GTTTGCATGT GTGACTTTGA GTTAAAGCAA GTACTCCGAG TGCTACCTTG TTCACATGAA 
TTTCATGCAA AATGTGTGGA TAAATGGCTA AGGTCAAATC GAACCTGCCC CATTTGTCGG 
GGTAATGCTT CGGACTACTT TGAAAATCCA GAGCAGCATC AACAGCAACA GCAGCAAACT 
CAACAGACAC AGTCTCAAAT CCAACCACAA TCTTCATCGC AACAATCTCA AACGCAGCCA 
GCAGCTACAA CACAAAATGC GATTTCAGGA GCGGCACAGC CGACAGCCCA ACAACAAACA 
CAAGCAACCC ATGCACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893638.1
CDS	245..3745
Translation	MMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_029037805.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037805.1

ORF Insert Sequence:

ATGATGCACC AACTTTTGAA CAACCACCAC AAAAAATTGC GCCGAACAAA ATTTCAATCC 
GAACATCGTA TGTTAATAAA AATTACAAAA ATGACAATCA CATCTGTATC ATTTTCAAAA 
CATCAATCAC AACAACGGCT TCTATCGCAA CATCCACGCA CTGTGACTGC ATCTCTAATG 
TCTTCTCGAT ATTTATCGAC AATTACCACA CCAATGTCAC CTACAATGGT ATCTAAACGA 
TTAATATGTA ATGAAAAAAA TAAAATAACA TCAATAATGA ATGCACTTGT CATAATGATG 
ACAACAACAA TGCTCCCACC ATCTATGGGG ATGGCAACAA TATCTGCAAC TTCAACATAT 
TCAACGATAA CTTCAACACC GTCAATTATT CAAAAACCAC GAAACCTCGA TCTCATTCGA 
AACCCACCCT TTTCCGATTT CATTTCTACG CTTTCTAATT GTAATCATTC CACATCTATT 
TCTACCCGAA TTTCAACTTT TGTGCCCGTT TTTTCCAACG ATTTTTTCAC ATCTATTTCT 
GAAATGTCTA CGATCTCATT GGGAACATCG TATGCTCTTG ATTTTGCATC TTCCTCCATC 
TTGACGTCGG CGCGCGTTAT CAAACTGAAT TCAACAAATA ACGAAAACAA TAACAATCAT 
AATCACATTC TAAATAAACA AAATTACAAT AATAATAATA AAACACATTT AAAATACTCA 
AACGATTGCG ATTCGACTTG TGGTACCGCC AATGGTAATA ATATTAATAA CAACAGTAGC 
AACAACAACA ACGTCAACAA TAATAACCAT CATCAGCAAT TCAATAATTT GAGTATGGGA 
CAGCAATCTC ATCTACGGCA TATACGTTTT AGCAATAACG GGCGTGGCAT GGATAATGGC 
AGTAACCATA ATGAATATAA TATTAGTAAC AACAACAATT ACCGACGAGG GGGAAACCAA 
AGTAACAACA GTAGTGGCAA TAGTATCCCG ATGATGATCC ATCGTCACAA TAATGAATAT 
TATGGGAATA ATCATCACAA TTATCAACAC CATCAATATA ATGAACATAG TCGTCGACAC 
AATGTCATTA ACGGCAACAA CAATAATAAC AATAGCAATA GCAGTAATGC GTCTAACTCA 
GGATTATTTG ATAGAAACCA TAATAATAAT AATAGTACCA ATAACAATGT AAATGTAAAT 
GTGAACGGGA ACGGGAACAA TGGCTGCAAC AACAACAGTG TGCATTTTGA TGGACTTAGT 
GGCAACAACC ATTCGCGGAG TGTTGATTTC CACACTGACA ATCGCACAGA ACGTGGCAGT 
ATAGCCGAGG CTACTAATAA TGGCAGTGGC GGTAACTTTA GCTACATCTC GGGACGAAAT 
AATAGCAATT ATGGGCGTAA CGGTCACCAA AATAACAGTT CTGGTAATTA CGGCTACCAT 
CATCATCATC ACTATAGCAA TAATGGGCCA TCAACATCAG GATTATCAGG CTTTAGCGGT 
AGTGTCTCCA ACCTTTTTGA TGGACCAAGT GGCTCTTCTG GTATTATGGG ATCCTCTTCT 
TCACTAAATA GTGGCCCCCA TGGGTTGAAA TCCGACAGTC CATCGCGAAA GAGGCGTCGC 
ATCTCTGGCC GCATGCCCAG CCAGTCACCG CCAGCGATAT GGGAGCAAAG ACGTTCTCCT 
CGCAATCAAA TGCAGCAGGG AAGTCCTCCG ATACGCCGCC CCCGTCTGCA TGATGCTGGG 
TCATCTCATC ATAACCGCGG CATTCATGGT AGCAACAATA GTGTTTCGGG TAATAGTATC 
AACAACACGA ATACAAATAA CGTTAGTGGA AACCATTTGC AAAGTTATCA CCAACCACAG 
CATTCAATAC AATCCACGCC ATATTACCTC CGGCAGCACA TGCCACCTCC ACCCGCTCTA 
CAACAACAAC AAACACCATC GCCGCCAACG CATCATCAAC CACAGTCCTT ACCACAGCCA 
CAATTACAGC AACGTTCACC TTGGGAGCAC TCATTATCTG CTGCTGCAGT TCTAACTAGC 
TCGAACCAAG CGCCTGCAGC CGTGAACGCT TATATGCAGC AAACTCCACC ACCACCACAG 
TCCCCTGGTC ATCATCACCA AACATCCTTG GTAGGAGCAC CACCGCCACC CCCACCTTTG 
ATGTTGGACA TCAATCAGGT TCCTGTCAGT CTTCCTTTAC GTCACGCCGA GCCTATATGG 
GCCTCTATAT GCACTTATCC AGCGCCACCG CCTCAAGCAA GAATAGCACC TTGTCATCTG 
CATGGTATTT ATACACAACC GTTCGCATCT CAAGCATGCA ATACACATCC CAGTGCTCAA 
TTCACGCAAG CCACGACTAG TTTCGCTGCT ACCGCCGGTA CACCAATTCA AATGCCACAA 
CCACAACAGG TCCAGGTTAA TGCAGCTGCA GCCGCACAAC AACAACACAC ACAACAAGCT 
GCCGCCCTTA TGGCAGCTGC TTCGGTTGCC GCGGCTAACT ACCAACATGC TCAATTTACC 
CAACAACAAC GTACGGAGGC TACTGCAGCC GCTGCGGCTG CTGCTGCTGC CGTAGCTGGT 
TTATCTCTAG AACAAGCCGG CGCTCATCAC CTAGACCCCC ATCAAGGACA TCAAGCTGCT 
GTACCGACTC CAACGGCACA TCACAATCAA TTACAAGCCA CACCAATTCA TATAACCTCC 
ATGTCAGCAG TTGCGGCTGC AGCAGCACAC CATCTGCGTA CTACTGCAAC AGCAGCGGCT 
CAAATGTCTG CTGCACCTCA ACCGATCTTG CTCTCTGCAG AGAGGCGTGT TTTTCCACCA 
CATCGTCGTA TTACCCGTTT TTGGCCAGCA AGTCATCCAC ACGGACCTCA CCGACATATG 
CTTTCACCAC AAACTTTGGG ACCACACCAA GCGACACCTG TACAAATTCA GACGACAACT 
GGAATCATTA ATCCGGGCTT TTTACTTAAT TTCCTTGCCA TGTTCCCGCT CTCACCTTAT 
AATCAACATG ATCTAAACTC ACTAGATTCA AACGAAACGG AAAATTACGA AGCTTTGTTA 
AGTTTGGCGG AACGTTTGGG TGAGGCTAAG CCACGCGGAT TGGCGCGAAA TGAAATCGAT 
CAGCTGCCAA GCTATAAGTA TAATCCAGAT ACCCATAGTG GTGATCAAAC GTCTTGCGTT 
GTTTGCATGT GTGACTTTGA GTTAAAGCAA GTACTCCGAG TGCTACCTTG TTCACATGAA 
TTTCATGCAA AATGTGTGGA TAAATGGCTA AGGTCAAATC GAACCTGCCC CATTTGTCGG 
GGTAATGCTT CGGACTACTT TGAAAATCCA GAGCAGCATC AACAGCAACA GCAGCAAACT 
CAACAGACAC AGTCTCAAAT CCAACCACAA TCTTCATCGC AACAATCTCA AACGCAGCCA 
GCAGCTACAA CACAAAATGC GATTTCAGGA GCGGCACAGC CGACAGCCCA ACAACAAACA 
CAAGCAACCC ATGCACATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178137.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176439.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176439.1
CDS	1609..6543
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178137.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178137.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178154.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176456.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176456.1
CDS	708..5642
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178154.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178154.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178143.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176445.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176445.1
CDS	1095..6029
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178143.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178143.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_029037804.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893637.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_029037804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	putative uncharacterized protein DDB_G0282133 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893637.1
CDS	604..5538
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_029037804.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037804.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178138.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176440.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176440.1
CDS	1596..6530
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178138.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178138.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178140.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176442.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176442.1
CDS	1658..6592
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178140.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178140.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178141.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176443.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176443.1
CDS	1393..6327
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178141.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178141.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178145.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176447.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176447.1
CDS	1350..6284
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178145.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178145.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178149.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176451.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176451.1
CDS	952..5886
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178149.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178149.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178152.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176454.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176454.1
CDS	749..5683
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178152.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178152.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178157.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176459.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176459.1
CDS	914..5848
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178157.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178157.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178159.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176461.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176461.1
CDS	545..5479
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178159.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178159.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_029037802.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893635.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_029037802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	putative uncharacterized protein DDB_G0282133 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893635.1
CDS	777..5711
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_029037802.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037802.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178148.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176450.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176450.1
CDS	1311..6245
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178148.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178148.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178150.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176452.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176452.1
CDS	935..5869
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178150.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178150.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178153.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176455.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176455.1
CDS	732..5666
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178153.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178153.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_029037803.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893636.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_029037803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	putative uncharacterized protein DDB_G0282133 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893636.1
CDS	573..5507
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_029037803.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037803.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178158.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176460.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	putative uncharacterized protein DDB_G0282133 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011178158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176460.1
CDS	591..5525
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178158.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178158.2

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178139.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176441.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176441.1
CDS	1519..6453
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178139.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178139.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178142.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176444.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176444.1
CDS	1329..6263
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178142.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178142.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178146.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176448.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176448.1
CDS	1356..6290
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178146.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178146.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178147.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176449.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176449.1
CDS	1077..6011
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178147.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178147.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178151.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176453.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176453.1
CDS	875..5809
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178151.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178151.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178156.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176458.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176458.1
CDS	712..5646
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178156.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178156.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_011178158.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176460.1 Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_011178158.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	probable serine/threonine-protein kinase DDB_G0282963 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176460.1
CDS	772..5706
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_011178158.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae probable serine/threonine-protein kinase DDB_G0282963 (LOC105208370), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178158.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208370 ( XM_029037801.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028893634.1 Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa194034
Clone ID Related Accession (Same CDS sequence)	XM_011178137.1 , XM_011178154.1 , XM_011178143.1 , XM_029037804.1 , XM_011178138.1 , XM_011178140.1 , XM_011178141.1 , XM_011178145.1 , XM_011178149.1 , XM_011178152.1 , XM_011178157.1 , XM_011178159.1 , XM_029037802.1 , XM_011178148.1 , XM_011178150.1 , XM_011178153.1 , XM_029037803.1 , XM_011178158.2 , XM_011178139.1 , XM_011178142.1 , XM_011178146.1 , XM_011178147.1 , XM_011178151.1 , XM_011178156.1 , XM_011178158.1 , XM_029037801.1
Accession Version	XM_029037801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	putative uncharacterized protein DDB_G0282133 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863672.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028893634.1
CDS	170..5104
Translation	MLNPNHTGATRRPFSRNALSRNQRCRLLFSPHNQSHHLLQQQQQLHVQPRSGSGNGNGNAASSSSTSSTTGHGGQWYSNKDSNIDNWNTLGDTQTSNNNSGSCTNNCQGNYYCHHYQHNNHHFHHSHNLQNHNNYNDHNPHQYCHNQLCPISTNYKRCPNHHRYNHNRIHQSHKQNRTTPNHCQYQTQEYTNQQYSYNISIKDSTLMLSFASASPAALLFPVTVNSNDNFLHFPNYNPNLPTHSRIRERLNNITISSQSFAVFDMNRYMTDYSMISSEKLNSQLSSLQISHPNSAASASMSMLITTFESSSNCQEPHSSPQINKILNINNYRHIPPDRCQSRNKIYNRSRQLYGNLGHNQNLFLHHANHNHYHNAINSYSNSDNHSRHYHQYNQSKNHHHHSSKIPTYQHHNHNQNIPTNHQSYLLLTNTNIIFTYTTKSTTTIENSNCTDDVNVTSCTDIISRRKSTRPPSPSRHQQMMHQLLNNHHKKLRRTKFQSEHRMLIKITKMTITSVSFSKHQSQQRLLSQHPRTVTASLMSSRYLSTITTPMSPTMVSKRLICNEKNKITSIMNALVIMMTTTMLPPSMGMATISATSTYSTITSTPSIIQKPRNLDLIRNPPFSDFISTLSNCNHSTSISTRISTFVPVFSNDFFTSISEMSTISLGTSYALDFASSSILTSARVIKLNSTNNENNNNHNHILNKQNYNNNNKTHLKYSNDCDSTCGTANGNNINNNSSNNNNVNNNNHHQQFNNLSMGQQSHLRHIRFSNNGRGMDNGSNHNEYNISNNNNYRRGGNQSNNSSGNSIPMMIHRHNNEYYGNNHHNYQHHQYNEHSRRHNVINGNNNNNNSNSSNASNSGLFDRNHNNNNSTNNNVNVNVNGNGNNGCNNNSVHFDGLSGNNHSRSVDFHTDNRTERGSIAEATNNGSGGNFSYISGRNNSNYGRNGHQNNSSGNYGYHHHHHYSNNGPSTSGLSGFSGSVSNLFDGPSGSSGIMGSSSSLNSGPHGLKSDSPSRKRRRISGRMPSQSPPAIWEQRRSPRNQMQQGSPPIRRPRLHDAGSSHHNRGIHGSNNSVSGNSINNTNTNNVSGNHLQSYHQPQHSIQSTPYYLRQHMPPPPALQQQQTPSPPTHHQPQSLPQPQLQQRSPWEHSLSAAAVLTSSNQAPAAVNAYMQQTPPPPQSPGHHHQTSLVGAPPPPPPLMLDINQVPVSLPLRHAEPIWASICTYPAPPPQARIAPCHLHGIYTQPFASQACNTHPSAQFTQATTSFAATAGTPIQMPQPQQVQVNAAAAAQQQHTQQAAALMAAASVAAANYQHAQFTQQQRTEATAAAAAAAAAVAGLSLEQAGAHHLDPHQGHQAAVPTPTAHHNQLQATPIHITSMSAVAAAAAHHLRTTATAAAQMSAAPQPILLSAERRVFPPHRRITRFWPASHPHGPHRHMLSPQTLGPHQATPVQIQTTTGIINPGFLLNFLAMFPLSPYNQHDLNSLDSNETENYEALLSLAERLGEAKPRGLARNEIDQLPSYKYNPDTHSGDQTSCVVCMCDFELKQVLRVLPCSHEFHAKCVDKWLRSNRTCPICRGNASDYFENPEQHQQQQQQTQQTQSQIQPQSSSQQSQTQPAATTQNAISGAAQPTAQQQTQATHAH

Target ORF information:

RefSeq Version	XM_029037801.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae putative uncharacterized protein DDB_G0282133 (LOC105208370), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029037801.1

ORF Insert Sequence:

ATGTTGAACC CTAATCACAC GGGAGCCACG CGACGTCCTT TCTCGCGGAA TGCACTGTCT 
CGGAATCAAC GTTGCCGTCT GCTATTCTCC CCGCATAATC AATCGCATCA CTTGCTGCAA 
CAACAGCAGC AGCTACATGT ACAACCCCGA AGTGGAAGCG GAAATGGCAA CGGCAACGCG 
GCTAGCAGTA GCAGTACCAG TTCCACGACC GGACATGGTG GACAGTGGTA TAGTAACAAG 
GATAGCAATA TCGACAACTG GAATACACTC GGCGATACAC AAACAAGTAA TAATAATAGT 
GGAAGTTGTA CTAATAATTG CCAAGGAAAT TACTATTGCC ACCACTACCA ACACAATAAC 
CACCATTTCC ATCACAGTCA CAATCTTCAA AACCATAATA ATTATAACGA TCATAACCCT 
CACCAATATT GCCATAATCA GCTGTGTCCA ATTAGTACCA ATTATAAACG TTGTCCTAAT 
CATCATCGAT ATAATCATAA TAGAATACAT CAAAGCCACA AACAAAATCG AACAACACCC 
AATCACTGTC AATATCAAAC GCAAGAGTAT ACAAATCAGC AGTATAGCTA CAATATCTCT 
ATAAAAGATT CCACACTCAT GCTGAGTTTT GCATCCGCTT CACCAGCAGC ATTATTGTTT 
CCAGTTACCG TAAACTCCAA CGACAATTTT CTGCACTTCC CTAATTACAA TCCCAATCTG 
CCGACCCATT CACGAATTCG TGAACGTTTG AATAATATTA CGATCTCATC TCAGTCATTC 
GCTGTTTTTG ATATGAATAG ATATATGACT GATTATTCAA TGATATCAAG TGAGAAACTC 
AACAGCCAGC TTTCATCTCT CCAGATTTCC CATCCAAATA GTGCAGCTTC AGCTAGTATG 
TCCATGTTGA TAACAACTTT TGAATCTTCT AGTAATTGTC AAGAACCGCA TTCAAGTCCT 
CAAATTAATA AAATTTTAAA TATAAACAAC TATAGACACA TACCACCAGA TCGATGCCAA 
AGTCGTAACA AAATTTACAA TCGCAGCCGA CAATTATACG GCAATCTTGG CCACAATCAA 
AATCTGTTTT TGCATCACGC TAACCATAAT CATTATCACA ATGCAATTAA TTCATATAGC 
AATAGTGACA ATCATAGTAG ACATTATCAT CAATATAATC AAAGTAAAAA TCACCATCAC 
CACTCGAGCA AAATCCCAAC ATACCAACAT CACAACCACA ACCAGAATAT ACCGACTAAT 
CACCAATCCT ACCTCCTGCT AACAAACACT AATATAATTT TCACATACAC TACAAAGTCT 
ACAACAACAA TTGAAAATAG TAATTGTACT GATGATGTGA ATGTAACTTC TTGTACTGAC 
ATAATTTCTC GACGGAAATC AACACGTCCA CCATCGCCTT CACGACACCA ACAAATGATG 
CACCAACTTT TGAACAACCA CCACAAAAAA TTGCGCCGAA CAAAATTTCA ATCCGAACAT 
CGTATGTTAA TAAAAATTAC AAAAATGACA ATCACATCTG TATCATTTTC AAAACATCAA 
TCACAACAAC GGCTTCTATC GCAACATCCA CGCACTGTGA CTGCATCTCT AATGTCTTCT 
CGATATTTAT CGACAATTAC CACACCAATG TCACCTACAA TGGTATCTAA ACGATTAATA 
TGTAATGAAA AAAATAAAAT AACATCAATA ATGAATGCAC TTGTCATAAT GATGACAACA 
ACAATGCTCC CACCATCTAT GGGGATGGCA ACAATATCTG CAACTTCAAC ATATTCAACG 
ATAACTTCAA CACCGTCAAT TATTCAAAAA CCACGAAACC TCGATCTCAT TCGAAACCCA 
CCCTTTTCCG ATTTCATTTC TACGCTTTCT AATTGTAATC ATTCCACATC TATTTCTACC 
CGAATTTCAA CTTTTGTGCC CGTTTTTTCC AACGATTTTT TCACATCTAT TTCTGAAATG 
TCTACGATCT CATTGGGAAC ATCGTATGCT CTTGATTTTG CATCTTCCTC CATCTTGACG 
TCGGCGCGCG TTATCAAACT GAATTCAACA AATAACGAAA ACAATAACAA TCATAATCAC 
ATTCTAAATA AACAAAATTA CAATAATAAT AATAAAACAC ATTTAAAATA CTCAAACGAT 
TGCGATTCGA CTTGTGGTAC CGCCAATGGT AATAATATTA ATAACAACAG TAGCAACAAC 
AACAACGTCA ACAATAATAA CCATCATCAG CAATTCAATA ATTTGAGTAT GGGACAGCAA 
TCTCATCTAC GGCATATACG TTTTAGCAAT AACGGGCGTG GCATGGATAA TGGCAGTAAC 
CATAATGAAT ATAATATTAG TAACAACAAC AATTACCGAC GAGGGGGAAA CCAAAGTAAC 
AACAGTAGTG GCAATAGTAT CCCGATGATG ATCCATCGTC ACAATAATGA ATATTATGGG 
AATAATCATC ACAATTATCA ACACCATCAA TATAATGAAC ATAGTCGTCG ACACAATGTC 
ATTAACGGCA ACAACAATAA TAACAATAGC AATAGCAGTA ATGCGTCTAA CTCAGGATTA 
TTTGATAGAA ACCATAATAA TAATAATAGT ACCAATAACA ATGTAAATGT AAATGTGAAC 
GGGAACGGGA ACAATGGCTG CAACAACAAC AGTGTGCATT TTGATGGACT TAGTGGCAAC 
AACCATTCGC GGAGTGTTGA TTTCCACACT GACAATCGCA CAGAACGTGG CAGTATAGCC 
GAGGCTACTA ATAATGGCAG TGGCGGTAAC TTTAGCTACA TCTCGGGACG AAATAATAGC 
AATTATGGGC GTAACGGTCA CCAAAATAAC AGTTCTGGTA ATTACGGCTA CCATCATCAT 
CATCACTATA GCAATAATGG GCCATCAACA TCAGGATTAT CAGGCTTTAG CGGTAGTGTC 
TCCAACCTTT TTGATGGACC AAGTGGCTCT TCTGGTATTA TGGGATCCTC TTCTTCACTA 
AATAGTGGCC CCCATGGGTT GAAATCCGAC AGTCCATCGC GAAAGAGGCG TCGCATCTCT 
GGCCGCATGC CCAGCCAGTC ACCGCCAGCG ATATGGGAGC AAAGACGTTC TCCTCGCAAT 
CAAATGCAGC AGGGAAGTCC TCCGATACGC CGCCCCCGTC TGCATGATGC TGGGTCATCT 
CATCATAACC GCGGCATTCA TGGTAGCAAC AATAGTGTTT CGGGTAATAG TATCAACAAC 
ACGAATACAA ATAACGTTAG TGGAAACCAT TTGCAAAGTT ATCACCAACC ACAGCATTCA 
ATACAATCCA CGCCATATTA CCTCCGGCAG CACATGCCAC CTCCACCCGC TCTACAACAA 
CAACAAACAC CATCGCCGCC AACGCATCAT CAACCACAGT CCTTACCACA GCCACAATTA 
CAGCAACGTT CACCTTGGGA GCACTCATTA TCTGCTGCTG CAGTTCTAAC TAGCTCGAAC 
CAAGCGCCTG CAGCCGTGAA CGCTTATATG CAGCAAACTC CACCACCACC ACAGTCCCCT 
GGTCATCATC ACCAAACATC CTTGGTAGGA GCACCACCGC CACCCCCACC TTTGATGTTG 
GACATCAATC AGGTTCCTGT CAGTCTTCCT TTACGTCACG CCGAGCCTAT ATGGGCCTCT 
ATATGCACTT ATCCAGCGCC ACCGCCTCAA GCAAGAATAG CACCTTGTCA TCTGCATGGT 
ATTTATACAC AACCGTTCGC ATCTCAAGCA TGCAATACAC ATCCCAGTGC TCAATTCACG 
CAAGCCACGA CTAGTTTCGC TGCTACCGCC GGTACACCAA TTCAAATGCC ACAACCACAA 
CAGGTCCAGG TTAATGCAGC TGCAGCCGCA CAACAACAAC ACACACAACA AGCTGCCGCC 
CTTATGGCAG CTGCTTCGGT TGCCGCGGCT AACTACCAAC ATGCTCAATT TACCCAACAA 
CAACGTACGG AGGCTACTGC AGCCGCTGCG GCTGCTGCTG CTGCCGTAGC TGGTTTATCT 
CTAGAACAAG CCGGCGCTCA TCACCTAGAC CCCCATCAAG GACATCAAGC TGCTGTACCG 
ACTCCAACGG CACATCACAA TCAATTACAA GCCACACCAA TTCATATAAC CTCCATGTCA 
GCAGTTGCGG CTGCAGCAGC ACACCATCTG CGTACTACTG CAACAGCAGC GGCTCAAATG 
TCTGCTGCAC CTCAACCGAT CTTGCTCTCT GCAGAGAGGC GTGTTTTTCC ACCACATCGT 
CGTATTACCC GTTTTTGGCC AGCAAGTCAT CCACACGGAC CTCACCGACA TATGCTTTCA 
CCACAAACTT TGGGACCACA CCAAGCGACA CCTGTACAAA TTCAGACGAC AACTGGAATC 
ATTAATCCGG GCTTTTTACT TAATTTCCTT GCCATGTTCC CGCTCTCACC TTATAATCAA 
CATGATCTAA ACTCACTAGA TTCAAACGAA ACGGAAAATT ACGAAGCTTT GTTAAGTTTG 
GCGGAACGTT TGGGTGAGGC TAAGCCACGC GGATTGGCGC GAAATGAAAT CGATCAGCTG 
CCAAGCTATA AGTATAATCC AGATACCCAT AGTGGTGATC AAACGTCTTG CGTTGTTTGC 
ATGTGTGACT TTGAGTTAAA GCAAGTACTC CGAGTGCTAC CTTGTTCACA TGAATTTCAT 
GCAAAATGTG TGGATAAATG GCTAAGGTCA AATCGAACCT GCCCCATTTG TCGGGGTAAT 
GCTTCGGACT ACTTTGAAAA TCCAGAGCAG CATCAACAGC AACAGCAGCA AACTCAACAG 
ACACAGTCTC AAATCCAACC ACAATCTTCA TCGCAACAAT CTCAAACGCA GCCAGCAGCT 
ACAACACAAA ATGCGATTTC AGGAGCGGCA CAGCCGACAG CCCAACAACA AACACAAGCA 
ACCCATGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC105208370 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: