pkp4 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_031892753.1	XP_031748613.1	plakophilin-4 isoform X3
XM_012970592.3	XP_012826046.1	plakophilin-4 isoform X4
XM_012970589.2	XP_012826043.1	plakophilin-4 isoform X2
XM_012970590.2	XP_012826044.1	plakophilin-4 isoform X3
XM_018097152.1	XP_017952641.1	plakophilin-4 isoform X5
XM_012970592.2	XP_012826046.1	plakophilin-4 isoform X1
XM_012970591.2	XP_012826045.1	plakophilin-4 isoform X4
NM_001270690.1	NP_001257619.1	plakophilin-4 isoform 1
XM_012970589.3	XP_012826043.1	plakophilin-4 isoform X1
XM_031892752.1	XP_031748612.1	plakophilin-4 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa46602	XM_018097152.1 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa29723	XM_012970592.3 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa29724	XM_012970589.3 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa29725	XM_031892752.1 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa29726	XM_031892753.1 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa01928	NM_001270690.1 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29725	XM_012970590.2 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29726	XM_012970591.2 Latest version!	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa29723	XM_012970592.2	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa29724	XM_012970589.2	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

pkp4 ( XM_018097152.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952641.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa46602
Clone ID Related Accession (Same CDS sequence)	XM_018097152.1
Accession Version	XM_018097152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGGCT TAAGAAGCTC ATATGCAAGT 
CAGCATAGCC AGCTCGGACA GGAACTGAGA TCAGCGGTAT CGCCCGACCT TCAGATCACG 
CCAATATATG AAGGGAGGAC GTATTACAGT CCAGTCTACC GGAACGCCAA CCACGCAGCC 
ATAGAGCTGA GGCAGGGCTC AAACACGGCA ATCTATCGGA CGGGATCAGG GTCCAGTAAC 
CTACAGAGGA CTATGAGCCA GCGGAGCACC CTTGCATACC AAAGAAATAA TTATGCGCTC 
AACTCAGCAG CTAATTATGC AGAGCCATAC AGATCAATGC AGTATCGAAT ATCGGAGCCC 
GGCTACAATA GGATGCCGCA TTCAGCGCCC CCTGATGATG GCACCACAAG GTCTCCTTCC 
ATTGACAGCA TCCAGAAGGA CCCAAGAGAG TTTGCTTGGC GGGACCCGGA GTTACCAGAA 
GTCATTCACA TGCTTCAGCA TCACTTTCCA TCGGTGCAAG CCAACGCCGC CGCATACCTG 
CAGCACCTCT GCTTTGGAGA CCACAAAACC AAGATAGAGG TGTGCCGGCT GGGCGGGATC 
AAGCACTTGG TAGACCTTCT CGATAACAAG GTGCTGGAAG TGCAGAAGAA CGCCTGTGGA 
GCCTTGAGAA ATTTAGTTTA TGGCAAATCT ACTGATGAAA ATAAGGTTGC CGTGAAAAAT 
GCAGGGGGTG TCCCTGCCCT TTTAAGACTA CTGAGAAAAA CAAATGACCA AGAAGTACGG 
GAACTTGTCA CTGGAGTCCT GTGGAACTTA TCTTCCTGTG ATGCTGTAAA GATGACTATT 
ATCCGTGACG CTTTATCTCC CCTCACTAAC ACTGTGATAG TGCCACATTC GGGCTTCAAT 
AATTCTTCTT TTGACGACGA TCACAAAATG AAATTTCAGT CTTCGTCGGT CCTGCGCAAC 
ACCACCGGCT GCATGAGGAA CCTGAGTTCG GCAGGGGAAG AGGCGCGCAA GCAGATGAGA 
TACTGTGAAG GGCTGGTCGA TTCTCTGCTC TTTGTGATCC AAACCTGTGT GAACACATCG 
GATTTTGACA GCAAGACGGT GGAGAACTGC GTATGCACGC TGAGAAACTT GTCCTACCGC 
CTGGAACTGG AAGTGCCTCA GGCTCGAATG CTGGGAATAA ACGAAATGGA CGGCTTCTGC 
AAGGAGTCCC CCAGCAAAGA GTCTGACTCC AGCTGTTGGG GGAAAAAAAA GAAAAAGAAA 
AAAAAGCCCC CGCAGGAAGA CTTGTGGGAT GGAGTTGGTC CTATTCCTGG GCTTTCCCAG 
TCCCCAAAAG GGGTGGAAAT GCTGTGGCAC CCATCGGTAG TCAAGCCATA TCTGACACTC 
CTAGCGGAAA GCTCTAACCC TGCAACGCTG GAGGGTTCTG CTGGGTCTTT GCAGAATCTG 
TCTGCTGGGA ATTGGAAGTT TGCAGCATAC ATCAGAGCTG CGGTACGGAA GGAGAAGGGA 
CTTCCTATTC TGGTCGAGCT GCTGAGAATG GATAATGATA GGGTCGTTTC CTCAGTCGCT 
ACAGCATTAA GAAATATGGC ATTAGATGTC CGCAACAAAG AGCTTATTGG TAAATACGCG 
ATGCGTGACT TAGTAAACAG ACTTCCCGGA GGTAACGGGC CGTGCGTTTT ATCTGACGAC 
ACAGTGGCTG CAATCTGTTG TGCATTGCAC GAGGTTACAA GCAAGAACAT GGAGAATGCG 
AAAGCGCTGG CCGACACCGG AGGGATTGAG AAGCTGGTGA ATATCACAAA AGGAAAGGGA 
GACAGATCTT CGTTGAAAGT GGTCAAAGCT GCTGCCCAAG TCCTAAACAC ATTATGGCAA 
TATCGAGACC TCCGTAGCAT TTATAAGAAG GACGGCTGGA ATCAGTCTCA TTTTATCACT 
CCAGTATCAA CACTAGAGCG TGAGCGCTTC AAGTCTCACC CGTCCTTATC TACCACCAAT 
CAGCAGATGT CTCCCATGAT CCAGTCAGTG GGCAGCACCT CCTCTTCACC AGCACTGTTA 
GGAATACGGG AACCTCACTC TGGTTATGAG AGAACACAAC CCTCTATGCA ATACTATAAC 
AGCCAGGGAG ACCCCATCTC TCAGAAGGAA ATGTTCACTG GGTCCAGCAA AGCATCTCCA 
ATCTACATCA GCTCCTATTC CTCACCAGCA CGAGAGCAAA ACAGGCGGCA GCAGCATCAC 
CAGCTGTACT ATAGTCTTGA AGACTCAAAT AGGAAAAACT ATGATGCATA CCGATTGTAT 
TTGCAGTCTC CACACAGCTA CGAAGACCCG TATTTTGACG AGCGCGTCCA CTACACGACG 
ACTGCAGATC AGAATGCGCA ATATGGACTC AAATCGACCA CAAACTATGT AGACTTTTAC 
TCCACTAGAA GACCTTCCTA TAGATCAGAA CCGTACCCGG GGTCGCCGGA CTCGTGGGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952641.1
CDS	181..2883
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_018097152.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097152.1

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGGCT TAAGAAGCTC ATATGCAAGT 
CAGCATAGCC AGCTCGGACA GGAACTGAGA TCAGCGGTAT CGCCCGACCT TCAGATCACG 
CCAATATATG AAGGGAGGAC GTATTACAGT CCAGTCTACC GGAACGCCAA CCACGCAGCC 
ATAGAGCTGA GGCAGGGCTC AAACACGGCA ATCTATCGGA CGGGATCAGG GTCCAGTAAC 
CTACAGAGGA CTATGAGCCA GCGGAGCACC CTTGCATACC AAAGAAATAA TTATGCGCTC 
AACTCAGCAG CTAATTATGC AGAGCCATAC AGATCAATGC AGTATCGAAT ATCGGAGCCC 
GGCTACAATA GGATGCCGCA TTCAGCGCCC CCTGATGATG GCACCACAAG GTCTCCTTCC 
ATTGACAGCA TCCAGAAGGA CCCAAGAGAG TTTGCTTGGC GGGACCCGGA GTTACCAGAA 
GTCATTCACA TGCTTCAGCA TCACTTTCCA TCGGTGCAAG CCAACGCCGC CGCATACCTG 
CAGCACCTCT GCTTTGGAGA CCACAAAACC AAGATAGAGG TGTGCCGGCT GGGCGGGATC 
AAGCACTTGG TAGACCTTCT CGATAACAAG GTGCTGGAAG TGCAGAAGAA CGCCTGTGGA 
GCCTTGAGAA ATTTAGTTTA TGGCAAATCT ACTGATGAAA ATAAGGTTGC CGTGAAAAAT 
GCAGGGGGTG TCCCTGCCCT TTTAAGACTA CTGAGAAAAA CAAATGACCA AGAAGTACGG 
GAACTTGTCA CTGGAGTCCT GTGGAACTTA TCTTCCTGTG ATGCTGTAAA GATGACTATT 
ATCCGTGACG CTTTATCTCC CCTCACTAAC ACTGTGATAG TGCCACATTC GGGCTTCAAT 
AATTCTTCTT TTGACGACGA TCACAAAATG AAATTTCAGT CTTCGTCGGT CCTGCGCAAC 
ACCACCGGCT GCATGAGGAA CCTGAGTTCG GCAGGGGAAG AGGCGCGCAA GCAGATGAGA 
TACTGTGAAG GGCTGGTCGA TTCTCTGCTC TTTGTGATCC AAACCTGTGT GAACACATCG 
GATTTTGACA GCAAGACGGT GGAGAACTGC GTATGCACGC TGAGAAACTT GTCCTACCGC 
CTGGAACTGG AAGTGCCTCA GGCTCGAATG CTGGGAATAA ACGAAATGGA CGGCTTCTGC 
AAGGAGTCCC CCAGCAAAGA GTCTGACTCC AGCTGTTGGG GGAAAAAAAA GAAAAAGAAA 
AAAAAGCCCC CGCAGGAAGA CTTGTGGGAT GGAGTTGGTC CTATTCCTGG GCTTTCCCAG 
TCCCCAAAAG GGGTGGAAAT GCTGTGGCAC CCATCGGTAG TCAAGCCATA TCTGACACTC 
CTAGCGGAAA GCTCTAACCC TGCAACGCTG GAGGGTTCTG CTGGGTCTTT GCAGAATCTG 
TCTGCTGGGA ATTGGAAGTT TGCAGCATAC ATCAGAGCTG CGGTACGGAA GGAGAAGGGA 
CTTCCTATTC TGGTCGAGCT GCTGAGAATG GATAATGATA GGGTCGTTTC CTCAGTCGCT 
ACAGCATTAA GAAATATGGC ATTAGATGTC CGCAACAAAG AGCTTATTGG TAAATACGCG 
ATGCGTGACT TAGTAAACAG ACTTCCCGGA GGTAACGGGC CGTGCGTTTT ATCTGACGAC 
ACAGTGGCTG CAATCTGTTG TGCATTGCAC GAGGTTACAA GCAAGAACAT GGAGAATGCG 
AAAGCGCTGG CCGACACCGG AGGGATTGAG AAGCTGGTGA ATATCACAAA AGGAAAGGGA 
GACAGATCTT CGTTGAAAGT GGTCAAAGCT GCTGCCCAAG TCCTAAACAC ATTATGGCAA 
TATCGAGACC TCCGTAGCAT TTATAAGAAG GACGGCTGGA ATCAGTCTCA TTTTATCACT 
CCAGTATCAA CACTAGAGCG TGAGCGCTTC AAGTCTCACC CGTCCTTATC TACCACCAAT 
CAGCAGATGT CTCCCATGAT CCAGTCAGTG GGCAGCACCT CCTCTTCACC AGCACTGTTA 
GGAATACGGG AACCTCACTC TGGTTATGAG AGAACACAAC CCTCTATGCA ATACTATAAC 
AGCCAGGGAG ACCCCATCTC TCAGAAGGAA ATGTTCACTG GGTCCAGCAA AGCATCTCCA 
ATCTACATCA GCTCCTATTC CTCACCAGCA CGAGAGCAAA ACAGGCGGCA GCAGCATCAC 
CAGCTGTACT ATAGTCTTGA AGACTCAAAT AGGAAAAACT ATGATGCATA CCGATTGTAT 
TTGCAGTCTC CACACAGCTA CGAAGACCCG TATTTTGACG AGCGCGTCCA CTACACGACG 
ACTGCAGATC AGAATGCGCA ATATGGACTC AAATCGACCA CAAACTATGT AGACTTTTAC 
TCCACTAGAA GACCTTCCTA TAGATCAGAA CCGTACCCGG GGTCGCCGGA CTCGTGGGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_012970592.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826046.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa29723
Clone ID Related Accession (Same CDS sequence)	XM_012970592.3 , XM_012970592.2
Accession Version	XM_012970592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGC ATCACCAGCT GTACTATAGT 
CTTGAAGACT CAAATAGGAA AAACTATGAT GCATACCGAT TGTATTTGCA GTCTCCACAC 
AGCTACGAAG ACCCGTATTT TGACGAGCGC GTCCACTACA CGACGACTGC AGATCAGAAT 
GCGCAATATG GACTCAAATC GACCACAAAC TATGTAGACT TTTACTCCAC TAGAAGACCT 
TCCTATAGAT CAGAACCGTA CCCGGGGTCG CCGGACTCGT GGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826046.1
CDS	187..3774
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHESEGSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_012970592.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970592.3

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGC ATCACCAGCT GTACTATAGT 
CTTGAAGACT CAAATAGGAA AAACTATGAT GCATACCGAT TGTATTTGCA GTCTCCACAC 
AGCTACGAAG ACCCGTATTT TGACGAGCGC GTCCACTACA CGACGACTGC AGATCAGAAT 
GCGCAATATG GACTCAAATC GACCACAAAC TATGTAGACT TTTACTCCAC TAGAAGACCT 
TCCTATAGAT CAGAACCGTA CCCGGGGTCG CCGGACTCGT GGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_012970589.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826043.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X1, mRNA.

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CloneID	OXa29724
Clone ID Related Accession (Same CDS sequence)	XM_012970589.2 , XM_012970589.3
Accession Version	XM_012970589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGGTCGGT GGGAAACTCC 
AGGAGCTCAA CTCAAATGAA TTCATACTCC GACAGCGGCT ACCAAGAAGC AGGCAGCTTT 
CATAACAACA ACATGGGGAA GGCCGAACTG CGAGCGCAGC TTTCGTTTAC AGGGTCCATG 
GGCAACCACG TCGTGCGGAA TGCCAGGGCG GAAGGCCAGA CTTTTGTCCA ACCACCACCA 
AATACAATGG CAGGCAGCCG GGCTGTGCGG CGGGTCAGCT CTGTCCCATC GAGGTCGCAG 
TCACCTTCTT ACGTCGTTGG ATCTGGAGTT TCTCCTTCTA GAGGGTCACT GAGAACTTCT 
GTCGGAAGTG GTTATGGTTC TCCGACAATT TCCGATCCAA GGCCGCTGGC CACTTCAAAC 
ACATACGCTT CTACTACATT GCCTTCTCAG AGAGCTGCTT CTCCTTATAC ATTGCATAGG 
TCCAACTCGC CTACAGTGGC GCGAAGGGTT GGTTCTGTTA ACAGCAGACA GCTGTCTAAT 
CCTTCATCTT ACCAGACATT GGGCGGTCCT GCCCGAGTTG GTTCACCATT GACTTTATCA 
GATGCACATA GTAGAGTTGG CTCATCATCC CAAACTCCTA TGGGATCCTC TTCCTCTCCA 
AAGCGGGCTG GTATGACTGC AGTACCGCAG CACATGGGAA CATTACAAAG AAATCTGCAT 
GATATCGATC AGTACGGACA GCAACATTTT GACATCTATG AAAGGATGGT CCCACCAAGA 
CCAGATAGCC TTACAGGCTT AAGAAGCTCA TATGCAAGTC AGCATAGCCA GCTCGGACAG 
GAACTGAGAT CAGCGGTATC GCCCGACCTT CAGATCACGC CAATATATGA AGGGAGGACG 
TATTACAGTC CAGTCTACCG GAACGCCAAC CACGCAGCCA TAGAGCTGAG GCAGGGCTCA 
AACACGGCAA TCTATCGGAC GGGATCAGGG TCCAGTAACC TACAGAGGAC TATGAGCCAG 
CGGAGCACCC TTGCATACCA AAGAAATAAT TATGCGCTCA ACTCAGCAGC TAATTATGCA 
GAGCCATACA GATCAATGCA GTATCGAATA TCGGAGCCCG GCTACAATAG GATGCCGCAT 
TCAGCGCCCC CTGATGATGG CACCACAAGG TCTCCTTCCA TTGACAGCAT CCAGAAGGAC 
CCAAGAGAGT TTGCTTGGCG GGACCCGGAG TTACCAGAAG TCATTCACAT GCTTCAGCAT 
CACTTTCCAT CGGTGCAAGC CAACGCCGCC GCATACCTGC AGCACCTCTG CTTTGGAGAC 
CACAAAACCA AGATAGAGGT GTGCCGGCTG GGCGGGATCA AGCACTTGGT AGACCTTCTC 
GATAACAAGG TGCTGGAAGT GCAGAAGAAC GCCTGTGGAG CCTTGAGAAA TTTAGTTTAT 
GGCAAATCTA CTGATGAAAA TAAGGTTGCC GTGAAAAATG CAGGGGGTGT CCCTGCCCTT 
TTAAGACTAC TGAGAAAAAC AAATGACCAA GAAGTACGGG AACTTGTCAC TGGAGTCCTG 
TGGAACTTAT CTTCCTGTGA TGCTGTAAAG ATGACTATTA TCCGTGACGC TTTATCTCCC 
CTCACTAACA CTGTGATAGT GCCACATTCG GGCTTCAATA ATTCTTCTTT TGACGACGAT 
CACAAAATGA AATTTCAGTC TTCGTCGGTC CTGCGCAACA CCACCGGCTG CATGAGGAAC 
CTGAGTTCGG CAGGGGAAGA GGCGCGCAAG CAGATGAGAT ACTGTGAAGG GCTGGTCGAT 
TCTCTGCTCT TTGTGATCCA AACCTGTGTG AACACATCGG ATTTTGACAG CAAGACGGTG 
GAGAACTGCG TATGCACGCT GAGAAACTTG TCCTACCGCC TGGAACTGGA AGTGCCTCAG 
GCTCGAATGC TGGGAATAAA CGAAATGGAC GGCTTCTGCA AGGAGTCCCC CAGCAAAGAG 
TCTGACTCCA GCTGTTGGGG GAAAAAAAAG AAAAAGAAAA AAAAGCCCCC GCAGGAAGAC 
TTGTGGGATG GAGTTGGTCC TATTCCTGGG CTTTCCCAGT CCCCAAAAGG GGTGGAAATG 
CTGTGGCACC CATCGGTAGT CAAGCCATAT CTGACACTCC TAGCGGAAAG CTCTAACCCT 
GCAACGCTGG AGGGTTCTGC TGGGTCTTTG CAGAATCTGT CTGCTGGGAA TTGGAAGTTT 
GCAGCATACA TCAGAGCTGC GGTACGGAAG GAGAAGGGAC TTCCTATTCT GGTCGAGCTG 
CTGAGAATGG ATAATGATAG GGTCGTTTCC TCAGTCGCTA CAGCATTAAG AAATATGGCA 
TTAGATGTCC GCAACAAAGA GCTTATTGGT AAATACGCGA TGCGTGACTT AGTAAACAGA 
CTTCCCGGAG GTAACGGGCC GTGCGTTTTA TCTGACGACA CAGTGGCTGC AATCTGTTGT 
GCATTGCACG AGGTTACAAG CAAGAACATG GAGAATGCGA AAGCGCTGGC CGACACCGGA 
GGGATTGAGA AGCTGGTGAA TATCACAAAA GGAAAGGGAG ACAGATCTTC GTTGAAAGTG 
GTCAAAGCTG CTGCCCAAGT CCTAAACACA TTATGGCAAT ATCGAGACCT CCGTAGCATT 
TATAAGAAGG ACGGCTGGAA TCAGTCTCAT TTTATCACTC CAGTATCAAC ACTAGAGCGT 
GAGCGCTTCA AGTCTCACCC GTCCTTATCT ACCACCAATC AGCAGATGTC TCCCATGATC 
CAGTCAGTGG GCAGCACCTC CTCTTCACCA GCACTGTTAG GAATACGGGA ACCTCACTCT 
GGTTATGAGA GAACACAACC CTCTATGCAA TACTATAACA GCCAGGGAGA CCCCATCTCT 
CAGAAGGAAA TGTTCACTGG GTCCAGCAAA GCATCTCCAA TCTACATCAG CTCCTATTCC 
TCACCAGCAC GAGAGCAAAA CAGGCGGCAG CAGCATCACC AGCTGTACTA TAGTCTTGAA 
GACTCAAATA GGAAAAACTA TGATGCATAC CGATTGTATT TGCAGTCTCC ACACAGCTAC 
GAAGACCCGT ATTTTGACGA GCGCGTCCAC TACACGACGA CTGCAGATCA GAATGCGCAA 
TATGGACTCA AATCGACCAC AAACTATGTA GACTTTTACT CCACTAGAAG ACCTTCCTAT 
AGATCAGAAC CGTACCCGGG GTCGCCGGAC TCGTGGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826043.1
CDS	181..3762
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHERSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_012970589.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970589.3

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGGTCGGT GGGAAACTCC 
AGGAGCTCAA CTCAAATGAA TTCATACTCC GACAGCGGCT ACCAAGAAGC AGGCAGCTTT 
CATAACAACA ACATGGGGAA GGCCGAACTG CGAGCGCAGC TTTCGTTTAC AGGGTCCATG 
GGCAACCACG TCGTGCGGAA TGCCAGGGCG GAAGGCCAGA CTTTTGTCCA ACCACCACCA 
AATACAATGG CAGGCAGCCG GGCTGTGCGG CGGGTCAGCT CTGTCCCATC GAGGTCGCAG 
TCACCTTCTT ACGTCGTTGG ATCTGGAGTT TCTCCTTCTA GAGGGTCACT GAGAACTTCT 
GTCGGAAGTG GTTATGGTTC TCCGACAATT TCCGATCCAA GGCCGCTGGC CACTTCAAAC 
ACATACGCTT CTACTACATT GCCTTCTCAG AGAGCTGCTT CTCCTTATAC ATTGCATAGG 
TCCAACTCGC CTACAGTGGC GCGAAGGGTT GGTTCTGTTA ACAGCAGACA GCTGTCTAAT 
CCTTCATCTT ACCAGACATT GGGCGGTCCT GCCCGAGTTG GTTCACCATT GACTTTATCA 
GATGCACATA GTAGAGTTGG CTCATCATCC CAAACTCCTA TGGGATCCTC TTCCTCTCCA 
AAGCGGGCTG GTATGACTGC AGTACCGCAG CACATGGGAA CATTACAAAG AAATCTGCAT 
GATATCGATC AGTACGGACA GCAACATTTT GACATCTATG AAAGGATGGT CCCACCAAGA 
CCAGATAGCC TTACAGGCTT AAGAAGCTCA TATGCAAGTC AGCATAGCCA GCTCGGACAG 
GAACTGAGAT CAGCGGTATC GCCCGACCTT CAGATCACGC CAATATATGA AGGGAGGACG 
TATTACAGTC CAGTCTACCG GAACGCCAAC CACGCAGCCA TAGAGCTGAG GCAGGGCTCA 
AACACGGCAA TCTATCGGAC GGGATCAGGG TCCAGTAACC TACAGAGGAC TATGAGCCAG 
CGGAGCACCC TTGCATACCA AAGAAATAAT TATGCGCTCA ACTCAGCAGC TAATTATGCA 
GAGCCATACA GATCAATGCA GTATCGAATA TCGGAGCCCG GCTACAATAG GATGCCGCAT 
TCAGCGCCCC CTGATGATGG CACCACAAGG TCTCCTTCCA TTGACAGCAT CCAGAAGGAC 
CCAAGAGAGT TTGCTTGGCG GGACCCGGAG TTACCAGAAG TCATTCACAT GCTTCAGCAT 
CACTTTCCAT CGGTGCAAGC CAACGCCGCC GCATACCTGC AGCACCTCTG CTTTGGAGAC 
CACAAAACCA AGATAGAGGT GTGCCGGCTG GGCGGGATCA AGCACTTGGT AGACCTTCTC 
GATAACAAGG TGCTGGAAGT GCAGAAGAAC GCCTGTGGAG CCTTGAGAAA TTTAGTTTAT 
GGCAAATCTA CTGATGAAAA TAAGGTTGCC GTGAAAAATG CAGGGGGTGT CCCTGCCCTT 
TTAAGACTAC TGAGAAAAAC AAATGACCAA GAAGTACGGG AACTTGTCAC TGGAGTCCTG 
TGGAACTTAT CTTCCTGTGA TGCTGTAAAG ATGACTATTA TCCGTGACGC TTTATCTCCC 
CTCACTAACA CTGTGATAGT GCCACATTCG GGCTTCAATA ATTCTTCTTT TGACGACGAT 
CACAAAATGA AATTTCAGTC TTCGTCGGTC CTGCGCAACA CCACCGGCTG CATGAGGAAC 
CTGAGTTCGG CAGGGGAAGA GGCGCGCAAG CAGATGAGAT ACTGTGAAGG GCTGGTCGAT 
TCTCTGCTCT TTGTGATCCA AACCTGTGTG AACACATCGG ATTTTGACAG CAAGACGGTG 
GAGAACTGCG TATGCACGCT GAGAAACTTG TCCTACCGCC TGGAACTGGA AGTGCCTCAG 
GCTCGAATGC TGGGAATAAA CGAAATGGAC GGCTTCTGCA AGGAGTCCCC CAGCAAAGAG 
TCTGACTCCA GCTGTTGGGG GAAAAAAAAG AAAAAGAAAA AAAAGCCCCC GCAGGAAGAC 
TTGTGGGATG GAGTTGGTCC TATTCCTGGG CTTTCCCAGT CCCCAAAAGG GGTGGAAATG 
CTGTGGCACC CATCGGTAGT CAAGCCATAT CTGACACTCC TAGCGGAAAG CTCTAACCCT 
GCAACGCTGG AGGGTTCTGC TGGGTCTTTG CAGAATCTGT CTGCTGGGAA TTGGAAGTTT 
GCAGCATACA TCAGAGCTGC GGTACGGAAG GAGAAGGGAC TTCCTATTCT GGTCGAGCTG 
CTGAGAATGG ATAATGATAG GGTCGTTTCC TCAGTCGCTA CAGCATTAAG AAATATGGCA 
TTAGATGTCC GCAACAAAGA GCTTATTGGT AAATACGCGA TGCGTGACTT AGTAAACAGA 
CTTCCCGGAG GTAACGGGCC GTGCGTTTTA TCTGACGACA CAGTGGCTGC AATCTGTTGT 
GCATTGCACG AGGTTACAAG CAAGAACATG GAGAATGCGA AAGCGCTGGC CGACACCGGA 
GGGATTGAGA AGCTGGTGAA TATCACAAAA GGAAAGGGAG ACAGATCTTC GTTGAAAGTG 
GTCAAAGCTG CTGCCCAAGT CCTAAACACA TTATGGCAAT ATCGAGACCT CCGTAGCATT 
TATAAGAAGG ACGGCTGGAA TCAGTCTCAT TTTATCACTC CAGTATCAAC ACTAGAGCGT 
GAGCGCTTCA AGTCTCACCC GTCCTTATCT ACCACCAATC AGCAGATGTC TCCCATGATC 
CAGTCAGTGG GCAGCACCTC CTCTTCACCA GCACTGTTAG GAATACGGGA ACCTCACTCT 
GGTTATGAGA GAACACAACC CTCTATGCAA TACTATAACA GCCAGGGAGA CCCCATCTCT 
CAGAAGGAAA TGTTCACTGG GTCCAGCAAA GCATCTCCAA TCTACATCAG CTCCTATTCC 
TCACCAGCAC GAGAGCAAAA CAGGCGGCAG CAGCATCACC AGCTGTACTA TAGTCTTGAA 
GACTCAAATA GGAAAAACTA TGATGCATAC CGATTGTATT TGCAGTCTCC ACACAGCTAC 
GAAGACCCGT ATTTTGACGA GCGCGTCCAC TACACGACGA CTGCAGATCA GAATGCGCAA 
TATGGACTCA AATCGACCAC AAACTATGTA GACTTTTACT CCACTAGAAG ACCTTCCTAT 
AGATCAGAAC CGTACCCGGG GTCGCCGGAC TCGTGGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_031892752.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748612.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa29725
Clone ID Related Accession (Same CDS sequence)	XM_012970590.2 , XM_031892752.1
Accession Version	XM_031892752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748612.1
CDS	6..3521
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHESEGSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_031892752.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892752.1

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_031892753.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748613.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X3, mRNA.

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CloneID	OXa29726
Clone ID Related Accession (Same CDS sequence)	XM_031892753.1 , XM_012970591.2
Accession Version	XM_031892753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGGTCCAGT AACCTACAGA GGACTATGAG CCAGCGGAGC 
ACCCTTGCAT ACCAAAGAAA TAATTATGCG CTCAACTCAG CAGCTAATTA TGCAGAGCCA 
TACAGATCAA TGCAGTATCG AATATCGGAG CCCGGCTACA ATAGGATGCC GCATTCAGCG 
CCCCCTGATG ATGGCACCAC AAGGTCTCCT TCCATTGACA GCATCCAGAA GGACCCAAGA 
GAGTTTGCTT GGCGGGACCC GGAGTTACCA GAAGTCATTC ACATGCTTCA GCATCACTTT 
CCATCGGTGC AAGCCAACGC CGCCGCATAC CTGCAGCACC TCTGCTTTGG AGACCACAAA 
ACCAAGATAG AGGTGTGCCG GCTGGGCGGG ATCAAGCACT TGGTAGACCT TCTCGATAAC 
AAGGTGCTGG AAGTGCAGAA GAACGCCTGT GGAGCCTTGA GAAATTTAGT TTATGGCAAA 
TCTACTGATG AAAATAAGGT TGCCGTGAAA AATGCAGGGG GTGTCCCTGC CCTTTTAAGA 
CTACTGAGAA AAACAAATGA CCAAGAAGTA CGGGAACTTG TCACTGGAGT CCTGTGGAAC 
TTATCTTCCT GTGATGCTGT AAAGATGACT ATTATCCGTG ACGCTTTATC TCCCCTCACT 
AACACTGTGA TAGTGCCACA TTCGGGCTTC AATAATTCTT CTTTTGACGA CGATCACAAA 
ATGAAATTTC AGTCTTCGTC GGTCCTGCGC AACACCACCG GCTGCATGAG GAACCTGAGT 
TCGGCAGGGG AAGAGGCGCG CAAGCAGATG AGATACTGTG AAGGGCTGGT CGATTCTCTG 
CTCTTTGTGA TCCAAACCTG TGTGAACACA TCGGATTTTG ACAGCAAGAC GGTGGAGAAC 
TGCGTATGCA CGCTGAGAAA CTTGTCCTAC CGCCTGGAAC TGGAAGTGCC TCAGGCTCGA 
ATGCTGGGAA TAAACGAAAT GGACGGCTTC TGCAAGGAGT CCCCCAGCAA AGAGTCTGAC 
TCCAGCTGTT GGGGGAAAAA AAAGAAAAAG AAAAAAAAGC CCCCGCAGGA AGACTTGTGG 
GATGGAGTTG GTCCTATTCC TGGGCTTTCC CAGTCCCCAA AAGGGGTGGA AATGCTGTGG 
CACCCATCGG TAGTCAAGCC ATATCTGACA CTCCTAGCGG AAAGCTCTAA CCCTGCAACG 
CTGGAGGGTT CTGCTGGGTC TTTGCAGAAT CTGTCTGCTG GGAATTGGAA GTTTGCAGCA 
TACATCAGAG CTGCGGTACG GAAGGAGAAG GGACTTCCTA TTCTGGTCGA GCTGCTGAGA 
ATGGATAATG ATAGGGTCGT TTCCTCAGTC GCTACAGCAT TAAGAAATAT GGCATTAGAT 
GTCCGCAACA AAGAGCTTAT TGGTAAATAC GCGATGCGTG ACTTAGTAAA CAGACTTCCC 
GGAGGTAACG GGCCGTGCGT TTTATCTGAC GACACAGTGG CTGCAATCTG TTGTGCATTG 
CACGAGGTTA CAAGCAAGAA CATGGAGAAT GCGAAAGCGC TGGCCGACAC CGGAGGGATT 
GAGAAGCTGG TGAATATCAC AAAAGGAAAG GGAGACAGAT CTTCGTTGAA AGTGGTCAAA 
GCTGCTGCCC AAGTCCTAAA CACATTATGG CAATATCGAG ACCTCCGTAG CATTTATAAG 
AAGGACGGCT GGAATCAGTC TCATTTTATC ACTCCAGTAT CAACACTAGA GCGTGAGCGC 
TTCAAGTCTC ACCCGTCCTT ATCTACCACC AATCAGCAGA TGTCTCCCAT GATCCAGTCA 
GTGGGCAGCA CCTCCTCTTC ACCAGCACTG TTAGGAATAC GGGAACCTCA CTCTGGTTAT 
GAGAGAACAC AACCCTCTAT GCAATACTAT AACAGCCAGG GAGACCCCAT CTCTCAGAAG 
GAAATGTTCA CTGGGTCCAG CAAAGCATCT CCAATCTACA TCAGCTCCTA TTCCTCACCA 
GCACGAGAGC AAAACAGGCG GCAGCAGCAT CACCAGCTGT ACTATAGTCT TGAAGACTCA 
AATAGGAAAA ACTATGATGC ATACCGATTG TATTTGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748613.1
CDS	6..3401
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHESEGSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_031892753.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892753.1

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGGTCCAGT AACCTACAGA GGACTATGAG CCAGCGGAGC 
ACCCTTGCAT ACCAAAGAAA TAATTATGCG CTCAACTCAG CAGCTAATTA TGCAGAGCCA 
TACAGATCAA TGCAGTATCG AATATCGGAG CCCGGCTACA ATAGGATGCC GCATTCAGCG 
CCCCCTGATG ATGGCACCAC AAGGTCTCCT TCCATTGACA GCATCCAGAA GGACCCAAGA 
GAGTTTGCTT GGCGGGACCC GGAGTTACCA GAAGTCATTC ACATGCTTCA GCATCACTTT 
CCATCGGTGC AAGCCAACGC CGCCGCATAC CTGCAGCACC TCTGCTTTGG AGACCACAAA 
ACCAAGATAG AGGTGTGCCG GCTGGGCGGG ATCAAGCACT TGGTAGACCT TCTCGATAAC 
AAGGTGCTGG AAGTGCAGAA GAACGCCTGT GGAGCCTTGA GAAATTTAGT TTATGGCAAA 
TCTACTGATG AAAATAAGGT TGCCGTGAAA AATGCAGGGG GTGTCCCTGC CCTTTTAAGA 
CTACTGAGAA AAACAAATGA CCAAGAAGTA CGGGAACTTG TCACTGGAGT CCTGTGGAAC 
TTATCTTCCT GTGATGCTGT AAAGATGACT ATTATCCGTG ACGCTTTATC TCCCCTCACT 
AACACTGTGA TAGTGCCACA TTCGGGCTTC AATAATTCTT CTTTTGACGA CGATCACAAA 
ATGAAATTTC AGTCTTCGTC GGTCCTGCGC AACACCACCG GCTGCATGAG GAACCTGAGT 
TCGGCAGGGG AAGAGGCGCG CAAGCAGATG AGATACTGTG AAGGGCTGGT CGATTCTCTG 
CTCTTTGTGA TCCAAACCTG TGTGAACACA TCGGATTTTG ACAGCAAGAC GGTGGAGAAC 
TGCGTATGCA CGCTGAGAAA CTTGTCCTAC CGCCTGGAAC TGGAAGTGCC TCAGGCTCGA 
ATGCTGGGAA TAAACGAAAT GGACGGCTTC TGCAAGGAGT CCCCCAGCAA AGAGTCTGAC 
TCCAGCTGTT GGGGGAAAAA AAAGAAAAAG AAAAAAAAGC CCCCGCAGGA AGACTTGTGG 
GATGGAGTTG GTCCTATTCC TGGGCTTTCC CAGTCCCCAA AAGGGGTGGA AATGCTGTGG 
CACCCATCGG TAGTCAAGCC ATATCTGACA CTCCTAGCGG AAAGCTCTAA CCCTGCAACG 
CTGGAGGGTT CTGCTGGGTC TTTGCAGAAT CTGTCTGCTG GGAATTGGAA GTTTGCAGCA 
TACATCAGAG CTGCGGTACG GAAGGAGAAG GGACTTCCTA TTCTGGTCGA GCTGCTGAGA 
ATGGATAATG ATAGGGTCGT TTCCTCAGTC GCTACAGCAT TAAGAAATAT GGCATTAGAT 
GTCCGCAACA AAGAGCTTAT TGGTAAATAC GCGATGCGTG ACTTAGTAAA CAGACTTCCC 
GGAGGTAACG GGCCGTGCGT TTTATCTGAC GACACAGTGG CTGCAATCTG TTGTGCATTG 
CACGAGGTTA CAAGCAAGAA CATGGAGAAT GCGAAAGCGC TGGCCGACAC CGGAGGGATT 
GAGAAGCTGG TGAATATCAC AAAAGGAAAG GGAGACAGAT CTTCGTTGAA AGTGGTCAAA 
GCTGCTGCCC AAGTCCTAAA CACATTATGG CAATATCGAG ACCTCCGTAG CATTTATAAG 
AAGGACGGCT GGAATCAGTC TCATTTTATC ACTCCAGTAT CAACACTAGA GCGTGAGCGC 
TTCAAGTCTC ACCCGTCCTT ATCTACCACC AATCAGCAGA TGTCTCCCAT GATCCAGTCA 
GTGGGCAGCA CCTCCTCTTC ACCAGCACTG TTAGGAATAC GGGAACCTCA CTCTGGTTAT 
GAGAGAACAC AACCCTCTAT GCAATACTAT AACAGCCAGG GAGACCCCAT CTCTCAGAAG 
GAAATGTTCA CTGGGTCCAG CAAAGCATCT CCAATCTACA TCAGCTCCTA TTCCTCACCA 
GCACGAGAGC AAAACAGGCG GCAGCAGCAT CACCAGCTGT ACTATAGTCT TGAAGACTCA 
AATAGGAAAA ACTATGATGC ATACCGATTG TATTTGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( NM_001270690.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001257619.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant 1, mRNA.

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CloneID	OXa01928
Clone ID Related Accession (Same CDS sequence)	NM_001270690.1
Accession Version	NM_001270690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03035544.1, AAMC03035542.1 and AAMC03035540.1. On Apr 22, 2014 this sequence version replaced NM_001079421.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1696413, SAMEA1696416 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGGTCGGT GGGAAACTCC 
AGGAGCTCAA CTCAAATGAA TTCATACTCC GACAGCGGCT ACCAAGAAGC AGGCAGCTTT 
CATAACAACA ACATGGGGAA GGCCGAACTG CGAGCGCAGC TTTCGTTTAC AGGGTCCATG 
GGCAACCACG TCGTGCGGAA TGCCAGGGCG GAAGGCCAGA CTTTTGTCCA ACCACCACCA 
AATACAATGG CAGGCAGCCG GGCTGTGCGG CGGGTCAGCT CTGTCCCATC GAGGTCGCAG 
TCACCTTCTT ACGTCGTTGG ATCTGGAGTT TCTCCTTCTA GAGGGTCACT GAGAACTTCT 
GTCGGAAGTG GTTATGGTTC TCCGACAATT TCCGATCCAA GGCCGCTGGC CACTTCAAAC 
ACATACGCTT CTACTACATT GCCTTCTCAG AGAGCTGCTT CTCCTTATAC ATTGCATAGG 
TCCAACTCGC CTACAGTGGC GCGAAGGGTT GGTTCTGTTA ACAGCAGACA GCTGTCTAAT 
CCTTCATCTT ACCAGACATT GGGCGGTCCT GCCCGAGTTG GTTCACCATT GACTTTATCA 
GATGCACATA GTAGAGTTGG CTCATCATCC CAAACTCCTA TGGGATCCTC TTCCTCTCCA 
AAGCGGGCTG GTATGACTGC AGTACCGCAG CACATGGGAA CATTACAAAG AAATCTGCAT 
GATATCGATC AGTACGGACA GCAACATTTT GACATCTATG AAAGGATGGT CCCACCAAGA 
CCAGATAGCC TTACAGGCTT AAGAAGCTCA TATGCAAGTC AGCATAGCCA GCTCGGACAG 
GAACTGAGAT CAGCGGTATC GCCCGACCTT CAGATCACGC CAATATATGA AGGGAGGACG 
TATTACAGTC CAGTCTACCG GAACGCCAAC CACGCAGCCA TAGAGCTGAG GCAGGGCTCA 
AACACGGCAA TCTATCGGAC GGGATCAGGG TCCAGTAACC TACAGAGGAC TATGAGCCAG 
CGGAGCACCC TTGCATACCA AAGAAATAAT TATGCGCTCA ACTCAGCAGC TAATTATGCA 
GAGCCATACA GATCAATGCA GTATCGAATA TCGGAGCCCG GCTACAATAG GATGCCGCAT 
TCAGCGCCCC CTGATGATGG CACCACAAGG TCTCCTTCCA TTGACAGCAT CCAGAAGGAC 
CCAAGAGAGT TTGCTTGGCG GGACCCGGAG TTACCAGAAG TCATTCACAT GCTTCAGCAT 
CACTTTCCAT CGGTGCAAGC CAACGCCGCC GCATACCTGC AGCACCTCTG CTTTGGAGAC 
CACAAAACCA AGATAGAGGT GTGCCGGCTG GGCGGGATCA AGCACTTGGT AGACCTTCTC 
GATAACAAGG TGCTGGAAGT GCAGAAGAAC GCCTGTGGAG CCTTGAGAAA TTTAGTTTAT 
GGCAAATCTA CTGATGAAAA TAAGGTTGCC GTGAAAAATG CAGGGGGTGT CCCTGCCCTT 
TTAAGACTAC TGAGAAAAAC AAATGACCAA GAAGTACGGG AACTTGTCAC TGGAGTCCTG 
TGGAACTTAT CTTCCTGTGA TGCTGTAAAG ATGACTATTA TCCGTGACGC TTTATCTCCC 
CTCACTAACA CTGTGATAGT GCCACATTCG GGCTTCAATA ATTCTTCTTT TGACGACGAT 
CACAAAATGA AATTTCAGTC TTCGTCGGTC CTGCGCAACA CCACCGGCTG CATGAGGAAC 
CTGAGTTCGG CAGGGGAAGA GGCGCGCAAG CAGATGAGAT ACTGTGAAGG GCTGGTCGAT 
TCTCTGCTCT TTGTGATCCA AACCTGTGTG AACACATCGG ATTTTGACAG CAAGACGGTG 
GAGAACTGCG TATGCACGCT GAGAAACTTG TCCTACCGCC TGGAACTGGA AGTGCCTCAG 
GCTCGAATGC TGGGAATAAA CGAAATGGAC GGCTTCTGCA AGGAGTCCCC CAGCAAAGAG 
TCTGACTCCA GCTGTTGGGG GAAAAAAAAG AAAAAGAAAA AAAAGCCCCC GCAGGAAGAC 
TTGTGGGATG GAGTTGGTCC TATTCCTGGG CTTTCCCAGT CCCCAAAAGG GGTGGAAATG 
CTGTGGCACC CATCGGTAGT CAAGCCATAT CTGACACTCC TAGCGGAAAG CTCTAACCCT 
GCAACGCTGG AGGGTTCTGC TGGGTCTTTG CAGAATCTGT CTGCTGGGAA TTGGAAGTTT 
GCAGCATACA TCAGAGCTGC GGTACGGAAG GAGAAGGGAC TTCCTATTCT GGTCGAGCTG 
CTGAGAATGG ATAATGATAG GGTCGTTTCC TCAGTCGCTA CAGCATTAAG AAATATGGCA 
TTAGATGTCC GCAACAAAGA GCTTATTGGT AAATACGCGA TGCGTGACTT AGTAAACAGA 
CTTCCCGGAG GTAACGGGCC GTGCGTTTTA TCTGACGACA CAGTGGCTGC AATCTGTTGT 
GCATTGCACG AGGTTACAAG CAAGAACATG GAGAATGCGA AAGCGCTGGC CGACACCGGA 
GGGATTGAGA AGCTGGTGAA TATCACAAAA GGAAAGGGAG ACAGATCTTC GTTGAAAGTG 
GTCAAAGCTG CTGCCCAAGT CCTAAACACA TTATGGCAAT ATCGAGACCT CCGTAGCATT 
TATAAGAAGG ACGGCTGGAA TCAGTCTCAT TTTATCACTC CAGTATCAAC ACTAGAGCGT 
GAGCGCTTCA AGTCTCACCC GTCCTTATCT ACCACCAATC AGCAGATGTC TCCCATGATC 
CAGTCAGTGG GCAGCACCTC CTCTTCACCA GCACTGTTAG GAATACGGGA ACCTCACTCT 
GGTTATGAGA GAACACAACC CTCTATGCAA TACTATAACA GCCAGGGAGA CCCCATCTCT 
CAGAAGGAAA TGTTCACTGG GTCCAGCAAA GCATCTCCAA TCTACATCAG CTCCTATTCC 
TCACCAGCAC GAGAGCAAAA CAGGCGGCAG CAGTCTCCAC ACAGCTACGA AGACCCGTAT 
TTTGACGAGC GCGTCCACTA CACGACGACT GCAGATCAGA ATGCGCAATA TGGACTCAAA 
TCGACCACAA ACTATGTAGA CTTTTACTCC ACTAGAAGAC CTTCCTATAG ATCAGAACCG 
TACCCGGGGT CGCCGGACTC GTGGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257619.1
CDS	187..3696
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHERSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	NM_001270690.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270690.1

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGGTCGGT GGGAAACTCC 
AGGAGCTCAA CTCAAATGAA TTCATACTCC GACAGCGGCT ACCAAGAAGC AGGCAGCTTT 
CATAACAACA ACATGGGGAA GGCCGAACTG CGAGCGCAGC TTTCGTTTAC AGGGTCCATG 
GGCAACCACG TCGTGCGGAA TGCCAGGGCG GAAGGCCAGA CTTTTGTCCA ACCACCACCA 
AATACAATGG CAGGCAGCCG GGCTGTGCGG CGGGTCAGCT CTGTCCCATC GAGGTCGCAG 
TCACCTTCTT ACGTCGTTGG ATCTGGAGTT TCTCCTTCTA GAGGGTCACT GAGAACTTCT 
GTCGGAAGTG GTTATGGTTC TCCGACAATT TCCGATCCAA GGCCGCTGGC CACTTCAAAC 
ACATACGCTT CTACTACATT GCCTTCTCAG AGAGCTGCTT CTCCTTATAC ATTGCATAGG 
TCCAACTCGC CTACAGTGGC GCGAAGGGTT GGTTCTGTTA ACAGCAGACA GCTGTCTAAT 
CCTTCATCTT ACCAGACATT GGGCGGTCCT GCCCGAGTTG GTTCACCATT GACTTTATCA 
GATGCACATA GTAGAGTTGG CTCATCATCC CAAACTCCTA TGGGATCCTC TTCCTCTCCA 
AAGCGGGCTG GTATGACTGC AGTACCGCAG CACATGGGAA CATTACAAAG AAATCTGCAT 
GATATCGATC AGTACGGACA GCAACATTTT GACATCTATG AAAGGATGGT CCCACCAAGA 
CCAGATAGCC TTACAGGCTT AAGAAGCTCA TATGCAAGTC AGCATAGCCA GCTCGGACAG 
GAACTGAGAT CAGCGGTATC GCCCGACCTT CAGATCACGC CAATATATGA AGGGAGGACG 
TATTACAGTC CAGTCTACCG GAACGCCAAC CACGCAGCCA TAGAGCTGAG GCAGGGCTCA 
AACACGGCAA TCTATCGGAC GGGATCAGGG TCCAGTAACC TACAGAGGAC TATGAGCCAG 
CGGAGCACCC TTGCATACCA AAGAAATAAT TATGCGCTCA ACTCAGCAGC TAATTATGCA 
GAGCCATACA GATCAATGCA GTATCGAATA TCGGAGCCCG GCTACAATAG GATGCCGCAT 
TCAGCGCCCC CTGATGATGG CACCACAAGG TCTCCTTCCA TTGACAGCAT CCAGAAGGAC 
CCAAGAGAGT TTGCTTGGCG GGACCCGGAG TTACCAGAAG TCATTCACAT GCTTCAGCAT 
CACTTTCCAT CGGTGCAAGC CAACGCCGCC GCATACCTGC AGCACCTCTG CTTTGGAGAC 
CACAAAACCA AGATAGAGGT GTGCCGGCTG GGCGGGATCA AGCACTTGGT AGACCTTCTC 
GATAACAAGG TGCTGGAAGT GCAGAAGAAC GCCTGTGGAG CCTTGAGAAA TTTAGTTTAT 
GGCAAATCTA CTGATGAAAA TAAGGTTGCC GTGAAAAATG CAGGGGGTGT CCCTGCCCTT 
TTAAGACTAC TGAGAAAAAC AAATGACCAA GAAGTACGGG AACTTGTCAC TGGAGTCCTG 
TGGAACTTAT CTTCCTGTGA TGCTGTAAAG ATGACTATTA TCCGTGACGC TTTATCTCCC 
CTCACTAACA CTGTGATAGT GCCACATTCG GGCTTCAATA ATTCTTCTTT TGACGACGAT 
CACAAAATGA AATTTCAGTC TTCGTCGGTC CTGCGCAACA CCACCGGCTG CATGAGGAAC 
CTGAGTTCGG CAGGGGAAGA GGCGCGCAAG CAGATGAGAT ACTGTGAAGG GCTGGTCGAT 
TCTCTGCTCT TTGTGATCCA AACCTGTGTG AACACATCGG ATTTTGACAG CAAGACGGTG 
GAGAACTGCG TATGCACGCT GAGAAACTTG TCCTACCGCC TGGAACTGGA AGTGCCTCAG 
GCTCGAATGC TGGGAATAAA CGAAATGGAC GGCTTCTGCA AGGAGTCCCC CAGCAAAGAG 
TCTGACTCCA GCTGTTGGGG GAAAAAAAAG AAAAAGAAAA AAAAGCCCCC GCAGGAAGAC 
TTGTGGGATG GAGTTGGTCC TATTCCTGGG CTTTCCCAGT CCCCAAAAGG GGTGGAAATG 
CTGTGGCACC CATCGGTAGT CAAGCCATAT CTGACACTCC TAGCGGAAAG CTCTAACCCT 
GCAACGCTGG AGGGTTCTGC TGGGTCTTTG CAGAATCTGT CTGCTGGGAA TTGGAAGTTT 
GCAGCATACA TCAGAGCTGC GGTACGGAAG GAGAAGGGAC TTCCTATTCT GGTCGAGCTG 
CTGAGAATGG ATAATGATAG GGTCGTTTCC TCAGTCGCTA CAGCATTAAG AAATATGGCA 
TTAGATGTCC GCAACAAAGA GCTTATTGGT AAATACGCGA TGCGTGACTT AGTAAACAGA 
CTTCCCGGAG GTAACGGGCC GTGCGTTTTA TCTGACGACA CAGTGGCTGC AATCTGTTGT 
GCATTGCACG AGGTTACAAG CAAGAACATG GAGAATGCGA AAGCGCTGGC CGACACCGGA 
GGGATTGAGA AGCTGGTGAA TATCACAAAA GGAAAGGGAG ACAGATCTTC GTTGAAAGTG 
GTCAAAGCTG CTGCCCAAGT CCTAAACACA TTATGGCAAT ATCGAGACCT CCGTAGCATT 
TATAAGAAGG ACGGCTGGAA TCAGTCTCAT TTTATCACTC CAGTATCAAC ACTAGAGCGT 
GAGCGCTTCA AGTCTCACCC GTCCTTATCT ACCACCAATC AGCAGATGTC TCCCATGATC 
CAGTCAGTGG GCAGCACCTC CTCTTCACCA GCACTGTTAG GAATACGGGA ACCTCACTCT 
GGTTATGAGA GAACACAACC CTCTATGCAA TACTATAACA GCCAGGGAGA CCCCATCTCT 
CAGAAGGAAA TGTTCACTGG GTCCAGCAAA GCATCTCCAA TCTACATCAG CTCCTATTCC 
TCACCAGCAC GAGAGCAAAA CAGGCGGCAG CAGTCTCCAC ACAGCTACGA AGACCCGTAT 
TTTGACGAGC GCGTCCACTA CACGACGACT GCAGATCAGA ATGCGCAATA TGGACTCAAA 
TCGACCACAA ACTATGTAGA CTTTTACTCC ACTAGAAGAC CTTCCTATAG ATCAGAACCG 
TACCCGGGGT CGCCGGACTC GTGGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_012970592.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826046.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X1, mRNA.

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CloneID	OXa29723
Clone ID Related Accession (Same CDS sequence)	XM_012970592.3 , XM_012970592.2
Accession Version	XM_012970592.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGC ATCACCAGCT GTACTATAGT 
CTTGAAGACT CAAATAGGAA AAACTATGAT GCATACCGAT TGTATTTGCA GTCTCCACAC 
AGCTACGAAG ACCCGTATTT TGACGAGCGC GTCCACTACA CGACGACTGC AGATCAGAAT 
GCGCAATATG GACTCAAATC GACCACAAAC TATGTAGACT TTTACTCCAC TAGAAGACCT 
TCCTATAGAT CAGAACCGTA CCCGGGGTCG CCGGACTCGT GGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826046.1
CDS	181..3768
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHESEGSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_012970592.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970592.2

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGC ATCACCAGCT GTACTATAGT 
CTTGAAGACT CAAATAGGAA AAACTATGAT GCATACCGAT TGTATTTGCA GTCTCCACAC 
AGCTACGAAG ACCCGTATTT TGACGAGCGC GTCCACTACA CGACGACTGC AGATCAGAAT 
GCGCAATATG GACTCAAATC GACCACAAAC TATGTAGACT TTTACTCCAC TAGAAGACCT 
TCCTATAGAT CAGAACCGTA CCCGGGGTCG CCGGACTCGT GGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_012970589.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826043.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X2, mRNA.

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CloneID	OXa29724
Clone ID Related Accession (Same CDS sequence)	XM_012970589.2 , XM_012970589.3
Accession Version	XM_012970589.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGGTCGGT GGGAAACTCC 
AGGAGCTCAA CTCAAATGAA TTCATACTCC GACAGCGGCT ACCAAGAAGC AGGCAGCTTT 
CATAACAACA ACATGGGGAA GGCCGAACTG CGAGCGCAGC TTTCGTTTAC AGGGTCCATG 
GGCAACCACG TCGTGCGGAA TGCCAGGGCG GAAGGCCAGA CTTTTGTCCA ACCACCACCA 
AATACAATGG CAGGCAGCCG GGCTGTGCGG CGGGTCAGCT CTGTCCCATC GAGGTCGCAG 
TCACCTTCTT ACGTCGTTGG ATCTGGAGTT TCTCCTTCTA GAGGGTCACT GAGAACTTCT 
GTCGGAAGTG GTTATGGTTC TCCGACAATT TCCGATCCAA GGCCGCTGGC CACTTCAAAC 
ACATACGCTT CTACTACATT GCCTTCTCAG AGAGCTGCTT CTCCTTATAC ATTGCATAGG 
TCCAACTCGC CTACAGTGGC GCGAAGGGTT GGTTCTGTTA ACAGCAGACA GCTGTCTAAT 
CCTTCATCTT ACCAGACATT GGGCGGTCCT GCCCGAGTTG GTTCACCATT GACTTTATCA 
GATGCACATA GTAGAGTTGG CTCATCATCC CAAACTCCTA TGGGATCCTC TTCCTCTCCA 
AAGCGGGCTG GTATGACTGC AGTACCGCAG CACATGGGAA CATTACAAAG AAATCTGCAT 
GATATCGATC AGTACGGACA GCAACATTTT GACATCTATG AAAGGATGGT CCCACCAAGA 
CCAGATAGCC TTACAGGCTT AAGAAGCTCA TATGCAAGTC AGCATAGCCA GCTCGGACAG 
GAACTGAGAT CAGCGGTATC GCCCGACCTT CAGATCACGC CAATATATGA AGGGAGGACG 
TATTACAGTC CAGTCTACCG GAACGCCAAC CACGCAGCCA TAGAGCTGAG GCAGGGCTCA 
AACACGGCAA TCTATCGGAC GGGATCAGGG TCCAGTAACC TACAGAGGAC TATGAGCCAG 
CGGAGCACCC TTGCATACCA AAGAAATAAT TATGCGCTCA ACTCAGCAGC TAATTATGCA 
GAGCCATACA GATCAATGCA GTATCGAATA TCGGAGCCCG GCTACAATAG GATGCCGCAT 
TCAGCGCCCC CTGATGATGG CACCACAAGG TCTCCTTCCA TTGACAGCAT CCAGAAGGAC 
CCAAGAGAGT TTGCTTGGCG GGACCCGGAG TTACCAGAAG TCATTCACAT GCTTCAGCAT 
CACTTTCCAT CGGTGCAAGC CAACGCCGCC GCATACCTGC AGCACCTCTG CTTTGGAGAC 
CACAAAACCA AGATAGAGGT GTGCCGGCTG GGCGGGATCA AGCACTTGGT AGACCTTCTC 
GATAACAAGG TGCTGGAAGT GCAGAAGAAC GCCTGTGGAG CCTTGAGAAA TTTAGTTTAT 
GGCAAATCTA CTGATGAAAA TAAGGTTGCC GTGAAAAATG CAGGGGGTGT CCCTGCCCTT 
TTAAGACTAC TGAGAAAAAC AAATGACCAA GAAGTACGGG AACTTGTCAC TGGAGTCCTG 
TGGAACTTAT CTTCCTGTGA TGCTGTAAAG ATGACTATTA TCCGTGACGC TTTATCTCCC 
CTCACTAACA CTGTGATAGT GCCACATTCG GGCTTCAATA ATTCTTCTTT TGACGACGAT 
CACAAAATGA AATTTCAGTC TTCGTCGGTC CTGCGCAACA CCACCGGCTG CATGAGGAAC 
CTGAGTTCGG CAGGGGAAGA GGCGCGCAAG CAGATGAGAT ACTGTGAAGG GCTGGTCGAT 
TCTCTGCTCT TTGTGATCCA AACCTGTGTG AACACATCGG ATTTTGACAG CAAGACGGTG 
GAGAACTGCG TATGCACGCT GAGAAACTTG TCCTACCGCC TGGAACTGGA AGTGCCTCAG 
GCTCGAATGC TGGGAATAAA CGAAATGGAC GGCTTCTGCA AGGAGTCCCC CAGCAAAGAG 
TCTGACTCCA GCTGTTGGGG GAAAAAAAAG AAAAAGAAAA AAAAGCCCCC GCAGGAAGAC 
TTGTGGGATG GAGTTGGTCC TATTCCTGGG CTTTCCCAGT CCCCAAAAGG GGTGGAAATG 
CTGTGGCACC CATCGGTAGT CAAGCCATAT CTGACACTCC TAGCGGAAAG CTCTAACCCT 
GCAACGCTGG AGGGTTCTGC TGGGTCTTTG CAGAATCTGT CTGCTGGGAA TTGGAAGTTT 
GCAGCATACA TCAGAGCTGC GGTACGGAAG GAGAAGGGAC TTCCTATTCT GGTCGAGCTG 
CTGAGAATGG ATAATGATAG GGTCGTTTCC TCAGTCGCTA CAGCATTAAG AAATATGGCA 
TTAGATGTCC GCAACAAAGA GCTTATTGGT AAATACGCGA TGCGTGACTT AGTAAACAGA 
CTTCCCGGAG GTAACGGGCC GTGCGTTTTA TCTGACGACA CAGTGGCTGC AATCTGTTGT 
GCATTGCACG AGGTTACAAG CAAGAACATG GAGAATGCGA AAGCGCTGGC CGACACCGGA 
GGGATTGAGA AGCTGGTGAA TATCACAAAA GGAAAGGGAG ACAGATCTTC GTTGAAAGTG 
GTCAAAGCTG CTGCCCAAGT CCTAAACACA TTATGGCAAT ATCGAGACCT CCGTAGCATT 
TATAAGAAGG ACGGCTGGAA TCAGTCTCAT TTTATCACTC CAGTATCAAC ACTAGAGCGT 
GAGCGCTTCA AGTCTCACCC GTCCTTATCT ACCACCAATC AGCAGATGTC TCCCATGATC 
CAGTCAGTGG GCAGCACCTC CTCTTCACCA GCACTGTTAG GAATACGGGA ACCTCACTCT 
GGTTATGAGA GAACACAACC CTCTATGCAA TACTATAACA GCCAGGGAGA CCCCATCTCT 
CAGAAGGAAA TGTTCACTGG GTCCAGCAAA GCATCTCCAA TCTACATCAG CTCCTATTCC 
TCACCAGCAC GAGAGCAAAA CAGGCGGCAG CAGCATCACC AGCTGTACTA TAGTCTTGAA 
GACTCAAATA GGAAAAACTA TGATGCATAC CGATTGTATT TGCAGTCTCC ACACAGCTAC 
GAAGACCCGT ATTTTGACGA GCGCGTCCAC TACACGACGA CTGCAGATCA GAATGCGCAA 
TATGGACTCA AATCGACCAC AAACTATGTA GACTTTTACT CCACTAGAAG ACCTTCCTAT 
AGATCAGAAC CGTACCCGGG GTCGCCGGAC TCGTGGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826043.1
CDS	181..3762
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHERSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_012970589.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970589.2

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGGTCGGT GGGAAACTCC 
AGGAGCTCAA CTCAAATGAA TTCATACTCC GACAGCGGCT ACCAAGAAGC AGGCAGCTTT 
CATAACAACA ACATGGGGAA GGCCGAACTG CGAGCGCAGC TTTCGTTTAC AGGGTCCATG 
GGCAACCACG TCGTGCGGAA TGCCAGGGCG GAAGGCCAGA CTTTTGTCCA ACCACCACCA 
AATACAATGG CAGGCAGCCG GGCTGTGCGG CGGGTCAGCT CTGTCCCATC GAGGTCGCAG 
TCACCTTCTT ACGTCGTTGG ATCTGGAGTT TCTCCTTCTA GAGGGTCACT GAGAACTTCT 
GTCGGAAGTG GTTATGGTTC TCCGACAATT TCCGATCCAA GGCCGCTGGC CACTTCAAAC 
ACATACGCTT CTACTACATT GCCTTCTCAG AGAGCTGCTT CTCCTTATAC ATTGCATAGG 
TCCAACTCGC CTACAGTGGC GCGAAGGGTT GGTTCTGTTA ACAGCAGACA GCTGTCTAAT 
CCTTCATCTT ACCAGACATT GGGCGGTCCT GCCCGAGTTG GTTCACCATT GACTTTATCA 
GATGCACATA GTAGAGTTGG CTCATCATCC CAAACTCCTA TGGGATCCTC TTCCTCTCCA 
AAGCGGGCTG GTATGACTGC AGTACCGCAG CACATGGGAA CATTACAAAG AAATCTGCAT 
GATATCGATC AGTACGGACA GCAACATTTT GACATCTATG AAAGGATGGT CCCACCAAGA 
CCAGATAGCC TTACAGGCTT AAGAAGCTCA TATGCAAGTC AGCATAGCCA GCTCGGACAG 
GAACTGAGAT CAGCGGTATC GCCCGACCTT CAGATCACGC CAATATATGA AGGGAGGACG 
TATTACAGTC CAGTCTACCG GAACGCCAAC CACGCAGCCA TAGAGCTGAG GCAGGGCTCA 
AACACGGCAA TCTATCGGAC GGGATCAGGG TCCAGTAACC TACAGAGGAC TATGAGCCAG 
CGGAGCACCC TTGCATACCA AAGAAATAAT TATGCGCTCA ACTCAGCAGC TAATTATGCA 
GAGCCATACA GATCAATGCA GTATCGAATA TCGGAGCCCG GCTACAATAG GATGCCGCAT 
TCAGCGCCCC CTGATGATGG CACCACAAGG TCTCCTTCCA TTGACAGCAT CCAGAAGGAC 
CCAAGAGAGT TTGCTTGGCG GGACCCGGAG TTACCAGAAG TCATTCACAT GCTTCAGCAT 
CACTTTCCAT CGGTGCAAGC CAACGCCGCC GCATACCTGC AGCACCTCTG CTTTGGAGAC 
CACAAAACCA AGATAGAGGT GTGCCGGCTG GGCGGGATCA AGCACTTGGT AGACCTTCTC 
GATAACAAGG TGCTGGAAGT GCAGAAGAAC GCCTGTGGAG CCTTGAGAAA TTTAGTTTAT 
GGCAAATCTA CTGATGAAAA TAAGGTTGCC GTGAAAAATG CAGGGGGTGT CCCTGCCCTT 
TTAAGACTAC TGAGAAAAAC AAATGACCAA GAAGTACGGG AACTTGTCAC TGGAGTCCTG 
TGGAACTTAT CTTCCTGTGA TGCTGTAAAG ATGACTATTA TCCGTGACGC TTTATCTCCC 
CTCACTAACA CTGTGATAGT GCCACATTCG GGCTTCAATA ATTCTTCTTT TGACGACGAT 
CACAAAATGA AATTTCAGTC TTCGTCGGTC CTGCGCAACA CCACCGGCTG CATGAGGAAC 
CTGAGTTCGG CAGGGGAAGA GGCGCGCAAG CAGATGAGAT ACTGTGAAGG GCTGGTCGAT 
TCTCTGCTCT TTGTGATCCA AACCTGTGTG AACACATCGG ATTTTGACAG CAAGACGGTG 
GAGAACTGCG TATGCACGCT GAGAAACTTG TCCTACCGCC TGGAACTGGA AGTGCCTCAG 
GCTCGAATGC TGGGAATAAA CGAAATGGAC GGCTTCTGCA AGGAGTCCCC CAGCAAAGAG 
TCTGACTCCA GCTGTTGGGG GAAAAAAAAG AAAAAGAAAA AAAAGCCCCC GCAGGAAGAC 
TTGTGGGATG GAGTTGGTCC TATTCCTGGG CTTTCCCAGT CCCCAAAAGG GGTGGAAATG 
CTGTGGCACC CATCGGTAGT CAAGCCATAT CTGACACTCC TAGCGGAAAG CTCTAACCCT 
GCAACGCTGG AGGGTTCTGC TGGGTCTTTG CAGAATCTGT CTGCTGGGAA TTGGAAGTTT 
GCAGCATACA TCAGAGCTGC GGTACGGAAG GAGAAGGGAC TTCCTATTCT GGTCGAGCTG 
CTGAGAATGG ATAATGATAG GGTCGTTTCC TCAGTCGCTA CAGCATTAAG AAATATGGCA 
TTAGATGTCC GCAACAAAGA GCTTATTGGT AAATACGCGA TGCGTGACTT AGTAAACAGA 
CTTCCCGGAG GTAACGGGCC GTGCGTTTTA TCTGACGACA CAGTGGCTGC AATCTGTTGT 
GCATTGCACG AGGTTACAAG CAAGAACATG GAGAATGCGA AAGCGCTGGC CGACACCGGA 
GGGATTGAGA AGCTGGTGAA TATCACAAAA GGAAAGGGAG ACAGATCTTC GTTGAAAGTG 
GTCAAAGCTG CTGCCCAAGT CCTAAACACA TTATGGCAAT ATCGAGACCT CCGTAGCATT 
TATAAGAAGG ACGGCTGGAA TCAGTCTCAT TTTATCACTC CAGTATCAAC ACTAGAGCGT 
GAGCGCTTCA AGTCTCACCC GTCCTTATCT ACCACCAATC AGCAGATGTC TCCCATGATC 
CAGTCAGTGG GCAGCACCTC CTCTTCACCA GCACTGTTAG GAATACGGGA ACCTCACTCT 
GGTTATGAGA GAACACAACC CTCTATGCAA TACTATAACA GCCAGGGAGA CCCCATCTCT 
CAGAAGGAAA TGTTCACTGG GTCCAGCAAA GCATCTCCAA TCTACATCAG CTCCTATTCC 
TCACCAGCAC GAGAGCAAAA CAGGCGGCAG CAGCATCACC AGCTGTACTA TAGTCTTGAA 
GACTCAAATA GGAAAAACTA TGATGCATAC CGATTGTATT TGCAGTCTCC ACACAGCTAC 
GAAGACCCGT ATTTTGACGA GCGCGTCCAC TACACGACGA CTGCAGATCA GAATGCGCAA 
TATGGACTCA AATCGACCAC AAACTATGTA GACTTTTACT CCACTAGAAG ACCTTCCTAT 
AGATCAGAAC CGTACCCGGG GTCGCCGGAC TCGTGGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_012970590.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826044.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X3, mRNA.

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CloneID	OXa29725
Clone ID Related Accession (Same CDS sequence)	XM_012970590.2 , XM_031892752.1
Accession Version	XM_012970590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826044.1
CDS	181..3696
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHESEGSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLQITPIYEGRTYYSPVYRNANHAAIELRQGSNTAIYRTGSGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_012970590.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970590.2

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGCTTAAGA AGCTCATATG CAAGTCAGCA TAGCCAGCTC 
GGACAGGAAC TGAGATCAGC GGTATCGCCC GACCTTCAGA TCACGCCAAT ATATGAAGGG 
AGGACGTATT ACAGTCCAGT CTACCGGAAC GCCAACCACG CAGCCATAGA GCTGAGGCAG 
GGCTCAAACA CGGCAATCTA TCGGACGGGA TCAGGGTCCA GTAACCTACA GAGGACTATG 
AGCCAGCGGA GCACCCTTGC ATACCAAAGA AATAATTATG CGCTCAACTC AGCAGCTAAT 
TATGCAGAGC CATACAGATC AATGCAGTAT CGAATATCGG AGCCCGGCTA CAATAGGATG 
CCGCATTCAG CGCCCCCTGA TGATGGCACC ACAAGGTCTC CTTCCATTGA CAGCATCCAG 
AAGGACCCAA GAGAGTTTGC TTGGCGGGAC CCGGAGTTAC CAGAAGTCAT TCACATGCTT 
CAGCATCACT TTCCATCGGT GCAAGCCAAC GCCGCCGCAT ACCTGCAGCA CCTCTGCTTT 
GGAGACCACA AAACCAAGAT AGAGGTGTGC CGGCTGGGCG GGATCAAGCA CTTGGTAGAC 
CTTCTCGATA ACAAGGTGCT GGAAGTGCAG AAGAACGCCT GTGGAGCCTT GAGAAATTTA 
GTTTATGGCA AATCTACTGA TGAAAATAAG GTTGCCGTGA AAAATGCAGG GGGTGTCCCT 
GCCCTTTTAA GACTACTGAG AAAAACAAAT GACCAAGAAG TACGGGAACT TGTCACTGGA 
GTCCTGTGGA ACTTATCTTC CTGTGATGCT GTAAAGATGA CTATTATCCG TGACGCTTTA 
TCTCCCCTCA CTAACACTGT GATAGTGCCA CATTCGGGCT TCAATAATTC TTCTTTTGAC 
GACGATCACA AAATGAAATT TCAGTCTTCG TCGGTCCTGC GCAACACCAC CGGCTGCATG 
AGGAACCTGA GTTCGGCAGG GGAAGAGGCG CGCAAGCAGA TGAGATACTG TGAAGGGCTG 
GTCGATTCTC TGCTCTTTGT GATCCAAACC TGTGTGAACA CATCGGATTT TGACAGCAAG 
ACGGTGGAGA ACTGCGTATG CACGCTGAGA AACTTGTCCT ACCGCCTGGA ACTGGAAGTG 
CCTCAGGCTC GAATGCTGGG AATAAACGAA ATGGACGGCT TCTGCAAGGA GTCCCCCAGC 
AAAGAGTCTG ACTCCAGCTG TTGGGGGAAA AAAAAGAAAA AGAAAAAAAA GCCCCCGCAG 
GAAGACTTGT GGGATGGAGT TGGTCCTATT CCTGGGCTTT CCCAGTCCCC AAAAGGGGTG 
GAAATGCTGT GGCACCCATC GGTAGTCAAG CCATATCTGA CACTCCTAGC GGAAAGCTCT 
AACCCTGCAA CGCTGGAGGG TTCTGCTGGG TCTTTGCAGA ATCTGTCTGC TGGGAATTGG 
AAGTTTGCAG CATACATCAG AGCTGCGGTA CGGAAGGAGA AGGGACTTCC TATTCTGGTC 
GAGCTGCTGA GAATGGATAA TGATAGGGTC GTTTCCTCAG TCGCTACAGC ATTAAGAAAT 
ATGGCATTAG ATGTCCGCAA CAAAGAGCTT ATTGGTAAAT ACGCGATGCG TGACTTAGTA 
AACAGACTTC CCGGAGGTAA CGGGCCGTGC GTTTTATCTG ACGACACAGT GGCTGCAATC 
TGTTGTGCAT TGCACGAGGT TACAAGCAAG AACATGGAGA ATGCGAAAGC GCTGGCCGAC 
ACCGGAGGGA TTGAGAAGCT GGTGAATATC ACAAAAGGAA AGGGAGACAG ATCTTCGTTG 
AAAGTGGTCA AAGCTGCTGC CCAAGTCCTA AACACATTAT GGCAATATCG AGACCTCCGT 
AGCATTTATA AGAAGGACGG CTGGAATCAG TCTCATTTTA TCACTCCAGT ATCAACACTA 
GAGCGTGAGC GCTTCAAGTC TCACCCGTCC TTATCTACCA CCAATCAGCA GATGTCTCCC 
ATGATCCAGT CAGTGGGCAG CACCTCCTCT TCACCAGCAC TGTTAGGAAT ACGGGAACCT 
CACTCTGGTT ATGAGAGAAC ACAACCCTCT ATGCAATACT ATAACAGCCA GGGAGACCCC 
ATCTCTCAGA AGGAAATGTT CACTGGGTCC AGCAAAGCAT CTCCAATCTA CATCAGCTCC 
TATTCCTCAC CAGCACGAGA GCAAAACAGG CGGCAGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pkp4 ( XM_012970591.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826045.1 Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X4, mRNA.

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CloneID	OXa29726
Clone ID Related Accession (Same CDS sequence)	XM_031892753.1 , XM_012970591.2
Accession Version	XM_012970591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plakophilin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGGTCCAGT AACCTACAGA GGACTATGAG CCAGCGGAGC 
ACCCTTGCAT ACCAAAGAAA TAATTATGCG CTCAACTCAG CAGCTAATTA TGCAGAGCCA 
TACAGATCAA TGCAGTATCG AATATCGGAG CCCGGCTACA ATAGGATGCC GCATTCAGCG 
CCCCCTGATG ATGGCACCAC AAGGTCTCCT TCCATTGACA GCATCCAGAA GGACCCAAGA 
GAGTTTGCTT GGCGGGACCC GGAGTTACCA GAAGTCATTC ACATGCTTCA GCATCACTTT 
CCATCGGTGC AAGCCAACGC CGCCGCATAC CTGCAGCACC TCTGCTTTGG AGACCACAAA 
ACCAAGATAG AGGTGTGCCG GCTGGGCGGG ATCAAGCACT TGGTAGACCT TCTCGATAAC 
AAGGTGCTGG AAGTGCAGAA GAACGCCTGT GGAGCCTTGA GAAATTTAGT TTATGGCAAA 
TCTACTGATG AAAATAAGGT TGCCGTGAAA AATGCAGGGG GTGTCCCTGC CCTTTTAAGA 
CTACTGAGAA AAACAAATGA CCAAGAAGTA CGGGAACTTG TCACTGGAGT CCTGTGGAAC 
TTATCTTCCT GTGATGCTGT AAAGATGACT ATTATCCGTG ACGCTTTATC TCCCCTCACT 
AACACTGTGA TAGTGCCACA TTCGGGCTTC AATAATTCTT CTTTTGACGA CGATCACAAA 
ATGAAATTTC AGTCTTCGTC GGTCCTGCGC AACACCACCG GCTGCATGAG GAACCTGAGT 
TCGGCAGGGG AAGAGGCGCG CAAGCAGATG AGATACTGTG AAGGGCTGGT CGATTCTCTG 
CTCTTTGTGA TCCAAACCTG TGTGAACACA TCGGATTTTG ACAGCAAGAC GGTGGAGAAC 
TGCGTATGCA CGCTGAGAAA CTTGTCCTAC CGCCTGGAAC TGGAAGTGCC TCAGGCTCGA 
ATGCTGGGAA TAAACGAAAT GGACGGCTTC TGCAAGGAGT CCCCCAGCAA AGAGTCTGAC 
TCCAGCTGTT GGGGGAAAAA AAAGAAAAAG AAAAAAAAGC CCCCGCAGGA AGACTTGTGG 
GATGGAGTTG GTCCTATTCC TGGGCTTTCC CAGTCCCCAA AAGGGGTGGA AATGCTGTGG 
CACCCATCGG TAGTCAAGCC ATATCTGACA CTCCTAGCGG AAAGCTCTAA CCCTGCAACG 
CTGGAGGGTT CTGCTGGGTC TTTGCAGAAT CTGTCTGCTG GGAATTGGAA GTTTGCAGCA 
TACATCAGAG CTGCGGTACG GAAGGAGAAG GGACTTCCTA TTCTGGTCGA GCTGCTGAGA 
ATGGATAATG ATAGGGTCGT TTCCTCAGTC GCTACAGCAT TAAGAAATAT GGCATTAGAT 
GTCCGCAACA AAGAGCTTAT TGGTAAATAC GCGATGCGTG ACTTAGTAAA CAGACTTCCC 
GGAGGTAACG GGCCGTGCGT TTTATCTGAC GACACAGTGG CTGCAATCTG TTGTGCATTG 
CACGAGGTTA CAAGCAAGAA CATGGAGAAT GCGAAAGCGC TGGCCGACAC CGGAGGGATT 
GAGAAGCTGG TGAATATCAC AAAAGGAAAG GGAGACAGAT CTTCGTTGAA AGTGGTCAAA 
GCTGCTGCCC AAGTCCTAAA CACATTATGG CAATATCGAG ACCTCCGTAG CATTTATAAG 
AAGGACGGCT GGAATCAGTC TCATTTTATC ACTCCAGTAT CAACACTAGA GCGTGAGCGC 
TTCAAGTCTC ACCCGTCCTT ATCTACCACC AATCAGCAGA TGTCTCCCAT GATCCAGTCA 
GTGGGCAGCA CCTCCTCTTC ACCAGCACTG TTAGGAATAC GGGAACCTCA CTCTGGTTAT 
GAGAGAACAC AACCCTCTAT GCAATACTAT AACAGCCAGG GAGACCCCAT CTCTCAGAAG 
GAAATGTTCA CTGGGTCCAG CAAAGCATCT CCAATCTACA TCAGCTCCTA TTCCTCACCA 
GCACGAGAGC AAAACAGGCG GCAGCAGCAT CACCAGCTGT ACTATAGTCT TGAAGACTCA 
AATAGGAAAA ACTATGATGC ATACCGATTG TATTTGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826045.1
CDS	181..3576
Translation	MPVPEQTPLMEEALQKIPQAAPSNTGMEPETTATNILASVKEQELQFERLTRELEVERQIVANQLERCRIGAESPSIASVSSTEKSFPWRSGDVPAGGPSKPRLSDSSHPNHFLIRSEPEPMAMYSPEQSSLHESEGSVGNSRSSTQMNSYSDSGYQEAGSFHNNNMGKAELRAQLSFTGSMGNHVVRNARAEGQTFVQPPPNTMAGSRAVRRVSSVPSRSQSPSYVVGSGVSPSRGSLRTSVGSGYGSPTISDPRPLATSNTYASTTLPSQRAASPYTLHRSNSPTVARRVGSVNSRQLSNPSSYQTLGGPARVGSPLTLSDAHSRVGSSSQTPMGSSSSPKRAGMTAVPQHMGTLQRNLHDIDQYGQQHFDIYERMVPPRPDSLTGSSNLQRTMSQRSTLAYQRNNYALNSAANYAEPYRSMQYRISEPGYNRMPHSAPPDDGTTRSPSIDSIQKDPREFAWRDPELPEVIHMLQHHFPSVQANAAAYLQHLCFGDHKTKIEVCRLGGIKHLVDLLDNKVLEVQKNACGALRNLVYGKSTDENKVAVKNAGGVPALLRLLRKTNDQEVRELVTGVLWNLSSCDAVKMTIIRDALSPLTNTVIVPHSGFNNSSFDDDHKMKFQSSSVLRNTTGCMRNLSSAGEEARKQMRYCEGLVDSLLFVIQTCVNTSDFDSKTVENCVCTLRNLSYRLELEVPQARMLGINEMDGFCKESPSKESDSSCWGKKKKKKKKPPQEDLWDGVGPIPGLSQSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPCVLSDDTVAAICCALHEVTSKNMENAKALADTGGIEKLVNITKGKGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQSHFITPVSTLERERFKSHPSLSTTNQQMSPMIQSVGSTSSSPALLGIREPHSGYERTQPSMQYYNSQGDPISQKEMFTGSSKASPIYISSYSSPAREQNRRQQHHQLYYSLEDSNRKNYDAYRLYLQSPHSYEDPYFDERVHYTTTADQNAQYGLKSTTNYVDFYSTRRPSYRSEPYPGSPDSWV

Target ORF information:

RefSeq Version	XM_012970591.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plakophilin 4 (pkp4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970591.2

ORF Insert Sequence:

ATGCCAGTTC CGGAGCAGAC TCCCCTGATG GAGGAGGCAC TGCAGAAGAT CCCGCAGGCA 
GCACCCAGCA ATACTGGCAT GGAGCCTGAA ACCACAGCCA CTAACATTTT GGCATCTGTG 
AAGGAACAGG AGCTGCAGTT TGAAAGGCTC ACCAGAGAAC TTGAGGTGGA AAGACAGATC 
GTTGCCAATC AGCTGGAAAG ATGCCGAATT GGGGCAGAAT CACCGAGTAT CGCCAGCGTA 
AGTTCTACTG AAAAGTCATT TCCCTGGAGA TCGGGAGATG TGCCGGCCGG AGGTCCCTCT 
AAACCGAGGC TCTCAGATTC CAGTCATCCC AACCACTTCT TGATAAGATC AGAGCCGGAA 
CCGATGGCCA TGTACTCTCC TGAGCAAAGC TCTCTCCATG AAAGTGAGGG GTCGGTGGGA 
AACTCCAGGA GCTCAACTCA AATGAATTCA TACTCCGACA GCGGCTACCA AGAAGCAGGC 
AGCTTTCATA ACAACAACAT GGGGAAGGCC GAACTGCGAG CGCAGCTTTC GTTTACAGGG 
TCCATGGGCA ACCACGTCGT GCGGAATGCC AGGGCGGAAG GCCAGACTTT TGTCCAACCA 
CCACCAAATA CAATGGCAGG CAGCCGGGCT GTGCGGCGGG TCAGCTCTGT CCCATCGAGG 
TCGCAGTCAC CTTCTTACGT CGTTGGATCT GGAGTTTCTC CTTCTAGAGG GTCACTGAGA 
ACTTCTGTCG GAAGTGGTTA TGGTTCTCCG ACAATTTCCG ATCCAAGGCC GCTGGCCACT 
TCAAACACAT ACGCTTCTAC TACATTGCCT TCTCAGAGAG CTGCTTCTCC TTATACATTG 
CATAGGTCCA ACTCGCCTAC AGTGGCGCGA AGGGTTGGTT CTGTTAACAG CAGACAGCTG 
TCTAATCCTT CATCTTACCA GACATTGGGC GGTCCTGCCC GAGTTGGTTC ACCATTGACT 
TTATCAGATG CACATAGTAG AGTTGGCTCA TCATCCCAAA CTCCTATGGG ATCCTCTTCC 
TCTCCAAAGC GGGCTGGTAT GACTGCAGTA CCGCAGCACA TGGGAACATT ACAAAGAAAT 
CTGCATGATA TCGATCAGTA CGGACAGCAA CATTTTGACA TCTATGAAAG GATGGTCCCA 
CCAAGACCAG ATAGCCTTAC AGGGTCCAGT AACCTACAGA GGACTATGAG CCAGCGGAGC 
ACCCTTGCAT ACCAAAGAAA TAATTATGCG CTCAACTCAG CAGCTAATTA TGCAGAGCCA 
TACAGATCAA TGCAGTATCG AATATCGGAG CCCGGCTACA ATAGGATGCC GCATTCAGCG 
CCCCCTGATG ATGGCACCAC AAGGTCTCCT TCCATTGACA GCATCCAGAA GGACCCAAGA 
GAGTTTGCTT GGCGGGACCC GGAGTTACCA GAAGTCATTC ACATGCTTCA GCATCACTTT 
CCATCGGTGC AAGCCAACGC CGCCGCATAC CTGCAGCACC TCTGCTTTGG AGACCACAAA 
ACCAAGATAG AGGTGTGCCG GCTGGGCGGG ATCAAGCACT TGGTAGACCT TCTCGATAAC 
AAGGTGCTGG AAGTGCAGAA GAACGCCTGT GGAGCCTTGA GAAATTTAGT TTATGGCAAA 
TCTACTGATG AAAATAAGGT TGCCGTGAAA AATGCAGGGG GTGTCCCTGC CCTTTTAAGA 
CTACTGAGAA AAACAAATGA CCAAGAAGTA CGGGAACTTG TCACTGGAGT CCTGTGGAAC 
TTATCTTCCT GTGATGCTGT AAAGATGACT ATTATCCGTG ACGCTTTATC TCCCCTCACT 
AACACTGTGA TAGTGCCACA TTCGGGCTTC AATAATTCTT CTTTTGACGA CGATCACAAA 
ATGAAATTTC AGTCTTCGTC GGTCCTGCGC AACACCACCG GCTGCATGAG GAACCTGAGT 
TCGGCAGGGG AAGAGGCGCG CAAGCAGATG AGATACTGTG AAGGGCTGGT CGATTCTCTG 
CTCTTTGTGA TCCAAACCTG TGTGAACACA TCGGATTTTG ACAGCAAGAC GGTGGAGAAC 
TGCGTATGCA CGCTGAGAAA CTTGTCCTAC CGCCTGGAAC TGGAAGTGCC TCAGGCTCGA 
ATGCTGGGAA TAAACGAAAT GGACGGCTTC TGCAAGGAGT CCCCCAGCAA AGAGTCTGAC 
TCCAGCTGTT GGGGGAAAAA AAAGAAAAAG AAAAAAAAGC CCCCGCAGGA AGACTTGTGG 
GATGGAGTTG GTCCTATTCC TGGGCTTTCC CAGTCCCCAA AAGGGGTGGA AATGCTGTGG 
CACCCATCGG TAGTCAAGCC ATATCTGACA CTCCTAGCGG AAAGCTCTAA CCCTGCAACG 
CTGGAGGGTT CTGCTGGGTC TTTGCAGAAT CTGTCTGCTG GGAATTGGAA GTTTGCAGCA 
TACATCAGAG CTGCGGTACG GAAGGAGAAG GGACTTCCTA TTCTGGTCGA GCTGCTGAGA 
ATGGATAATG ATAGGGTCGT TTCCTCAGTC GCTACAGCAT TAAGAAATAT GGCATTAGAT 
GTCCGCAACA AAGAGCTTAT TGGTAAATAC GCGATGCGTG ACTTAGTAAA CAGACTTCCC 
GGAGGTAACG GGCCGTGCGT TTTATCTGAC GACACAGTGG CTGCAATCTG TTGTGCATTG 
CACGAGGTTA CAAGCAAGAA CATGGAGAAT GCGAAAGCGC TGGCCGACAC CGGAGGGATT 
GAGAAGCTGG TGAATATCAC AAAAGGAAAG GGAGACAGAT CTTCGTTGAA AGTGGTCAAA 
GCTGCTGCCC AAGTCCTAAA CACATTATGG CAATATCGAG ACCTCCGTAG CATTTATAAG 
AAGGACGGCT GGAATCAGTC TCATTTTATC ACTCCAGTAT CAACACTAGA GCGTGAGCGC 
TTCAAGTCTC ACCCGTCCTT ATCTACCACC AATCAGCAGA TGTCTCCCAT GATCCAGTCA 
GTGGGCAGCA CCTCCTCTTC ACCAGCACTG TTAGGAATAC GGGAACCTCA CTCTGGTTAT 
GAGAGAACAC AACCCTCTAT GCAATACTAT AACAGCCAGG GAGACCCCAT CTCTCAGAAG 
GAAATGTTCA CTGGGTCCAG CAAAGCATCT CCAATCTACA TCAGCTCCTA TTCCTCACCA 
GCACGAGAGC AAAACAGGCG GCAGCAGCAT CACCAGCTGT ACTATAGTCT TGAAGACTCA 
AATAGGAAAA ACTATGATGC ATACCGATTG TATTTGCAGT CTCCACACAG CTACGAAGAC 
CCGTATTTTG ACGAGCGCGT CCACTACACG ACGACTGCAG ATCAGAATGC GCAATATGGA 
CTCAAATCGA CCACAAACTA TGTAGACTTT TACTCCACTA GAAGACCTTC CTATAGATCA 
GAACCGTACC CGGGGTCGCC GGACTCGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	pkp4
Entrez Gene ID	780351
Full Name	plakophilin 4
Synonyms	p0071
General protein information	Preferred Names plakophilin 4 Names plakophilin-4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	PKP4	XP_001147223.1
Homo sapiens (human)	PKP4	NP_003619.2
	PKP4	XP_001091595.2
Canis lupus familiaris (dog)	PKP4	XP_848525.1
Xenopus tropicalis (tropical clawed frog)	pkp4	NP_001257619.1
Mus musculus (house mouse)	Pkp4	NP_080637.1
Bos taurus (cattle)	PKP4	NP_001178420.1
Rattus norvegicus (Norway rat)	Pkp4	XP_003749523.1
Gallus gallus (chicken)	PKP4	NP_001006529.1
Danio rerio (zebrafish)	pkp4	XP_005165904.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

pkp4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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