ORF » Species Summary » Xenopus tropicalis » ankfy1 cDNA ORF clone

ankfy1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ankfy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankfy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20597 NM_001079419.1
Latest version!		 Xenopus tropicalis ankyrin repeat and FYVE domain containing 1 (ankfy1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa29721 XM_012957332.1
Latest version!		 Xenopus tropicalis ankyrin repeat and FYVE domain containing 1 (ankfy1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ankfy1 ( NM_001079419.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072887.1 Xenopus tropicalis ankyrin repeat and FYVE domain containing 1 (ankfy1), mRNA.
    Price & Availability↑
    CloneID OXa20597
    Clone ID Related Accession (Same CDS sequence) NM_001079419.1
    Accession Version NM_001079419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rabankyrin-5
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125724.1. ##Evidence-Data-START## Transcript exon combination :: BC125724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028292 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAG AGGAGGTGGG AAAGCTGCAG AAGCATCTAG CGCTTCTCCG TCAGGAGTAT 
    GTCAAGTTGC AGCAAAAGCT GGCAGAGACT GAGAAAAAAT GCACTCTCCT AGCAGCCCAG 
    GCTAACAAAG AAGCCTCCAG CGACTCTTTC ATCAGCCGCC TCCTCTCTAT CGTGGCACAA 
    CTCTTTCAGC AGGAGCAGTA TAGTGATCTG ATGATAAAAG TTGGAGACAA ACACATAAAT 
    GCCCATAAAT TTGTTCTTGC TGCAAGAAGT GAGGTTTGGA GTCCAGCCAA TTTGGCTTCA 
    ACTAAAGAAC TTGACCTGTC AGACTCTAAT CCTGAGGTAA CTATGGCTAT GCTTCGTTGG 
    GTCTACACAG ATGAGCTGGA TTTAAGGGAA GATGATATAT TCTTAACTGA GTTGATGAAG 
    CTAGCTAATC GTTTCCAACT TCAACTACTT AGGGAAAGGT GTGAAAAGGG AGTTATGTCT 
    CTGGTGAATG TGAGGAACTG CATACGTTTT TATCAGACTG CTGAGGAGCT AAATGCCAAT 
    GCTTTGTTAA ACTACTGTGC TGAGATTATT GCAAGTCACT GGGATGACCT AAGAAAAGAG 
    GACTTCAGCT CAATGAGTGC TCAGCTACTA TATAAAATGA TTAAATCAAA GACACAGTTC 
    CCTTTGCACA AAGCAATAAA GTTGGAAAGA GAAGATGTAG TTTTCTTGTA CCTAATAGAA 
    ATGGATTCTC AGTTGCCTGG AAAACTAAAT GAACTGGACC AAAATGGAGA CCTTTCCTTG 
    GATTTAGCTC TTTCCCGGAG ACTGGAAAGC ATAGCAACTA CATTGGTTAA TAACAAAGCT 
    GATGTTGACA TGATGGACAA AAAGGGTTGC AGTTTATTAC ATAAAGCCAT TTGTAGAGAC 
    GATAAATTTG CTGCAAACTT TCTAATAAAA AATGGTGCTC TTGTAAATGC TGCCACTGTT 
    GGAGAGCAAC AGACTCCTCT GCATTTGGTA GCTTCTTGCA CAGTAAAGGA CCGGTCTGTG 
    GAAGTGATGT CCGATATAGC ACAAATTGCT GAAGCACTTT TAAAAGCAGG AGCAAATCCT 
    AACATGCAAG ACAGCAAAGG AAGGAGTCCT CTGCACGCTG CTGTTCTTGC TAGGAATGAG 
    TATGTCTTCA ACCAACTCCT TCTGTGCAAA CAATTAGATC TAGAGCTGAA GGATCATGAG 
    GGAAGCACAG CTCTGTGGCT TGCTGTTCAG TATATCACAG TGTCTTCTGA CCAGTCTGTA 
    AATCCTTTTG ATGATGTCCC TGTTGTAAAT GGCACCTCAT TTGATGAAAA CAGCTTTGCT 
    GCCAGACTTA TTAAACAGGG CAGCAACACA GATGCACCAG ACACTGTAAC AGGCAACTGC 
    TTGTTACAAA GAGCTGCTGC TGCAGGGAAT GAAGCTGCTT CCCTGTTCTT GGCAACCCAT 
    GGGGCAAAAG TCAATCATAA AAATAAATGG GGTGAATCGC CTTTACATAC TGCATGTCGG 
    CATGGACTTT CTAACCTTAC AGCAGAGCTT TTACAACAAG GAGCCAACCC AAATATTCAG 
    ACTCAAGAAC CTATTACTTC TCCAAGAGGA CCTCCAGCAA ACAGTCTGGC AGACAGCACT 
    TACTTACAAA CACCTCTGCA CATGGCAATC GCATATAACC ATCCTGATGT TGTTTCTGTC 
    ATATTGGAAC AAAAAGCTAA TGCTCTTCAT GCAACAAATA ATCTACAGAT CATACCTGAC 
    TTTAGTCTCA AGGACTCCAG AGAACAGACA GTGTTAGGAC TGGCACTATG GACAGGCATG 
    CACACCATTG CCGCACAACT CCTGGGCTCT GGAGCATCCA TCAATGATAC AATGTCAGAT 
    GGACAGACAC TTCTACACAT GGCAATACAA AGACAAGACA GCAAGAGTGC CCTGTTCCTG 
    CTAGAACACC AAGCTGATAT CAATGTCCGA ACCCGGGATG GCGAGACAGC ACTTCAGCTT 
    GCCATTAAAA ATCAGCTTCC TTTGGTGGTG GATGCCATTT GTACAAGAGG CGCTGATATG 
    TCTGTTACTG ATGAACAAGG GAATCCCCCT CTGTGGCTTG CACTAGAGAA TGAGCAGGAG 
    GATATTGCCT CAACTCTGGT AAGACATGGA TGTGATGCTA CATTCTGGGG GCCAGGGCCA 
    AGTGGATGCC AACAGACGCT ACTTCATAGA GCAATTGATG AAAACAAAGA GCAAATTGCT 
    TGTTTCATTA TTCGCAGTGG CTGTGATGTA AATAGCACTA GAAAGCCTGG ACCAAATGGA 
    GAAGGAGAAG AAGAGGCCAG AGATGGGCAA ACCCCCTTGC ATTTAGCAGC TTCTTGGGGA 
    CTGGAGGAGG TAGTGCAGTG CCTTTTGGAG TTTGGTGCCA ATGTAAATAC TCAGGATGCA 
    GAGGGGAGAA CTCCAATACA CGTTGCTATA AGCAACCAGC ATCGTGTCAT TATCCAGTTA 
    TTGATCTCAC ACCCTGACAT CCGGCTGAAT GTTCGTGACA GGCAGGGTAT GACCCCATTC 
    GCATGTGCCA TGACTTTCAA AAATAACAAG GCCGCCGAGG CAATTCTGAA ACGGGAACCA 
    GGAGCCGCTG AGCAGGTGGA TAACAAAGGG AGGAATTTCC TGCATGTTGC AGTTCAGAAT 
    TCAGATATTG AGAGTGTCTT GTTCTTGATA AGTGTTCAGG CTAACGTTAA TTCAAGAGTC 
    CAAGATGCCT CCAAGCTAAC CCCACTGCAT CTAGCTGTGC AAGCAGGATC AGAGATTATT 
    GTCCGGAACC TGCTTCTTGC AGGTGCAAAG GTGAATGAAC TTACCAAGCA TCGTCAGACT 
    GCCCTTCACC TAGCAGCACA ACAGGACCTT CCAGTTATTT GTTCTGTTCT CCTTGAGAAT 
    GGTGTTGACT TTAGTGCTGC AGATGAGAAT GGAAATAATG CTCTGCATCT GGCAATAATG 
    CATGGGCGCT TGAACAATAT ACGCTGCCTT TTAACTGAAT GCAATGTGGA TGCAGAGGCT 
    TTAAACTTAA GAGGTCAGTC CCCAATGCAT ATTTTGGGGC AATATGGAAA GGAAAATGCT 
    GCTGCCATCT CTGAACTCTT TCTGGAATGT ATGCCACAGT ATCCTCTTGA TAAACCAGAT 
    GCAGAAGGAA ACACTGTGCT GCTACTAGCG TATATGAAGG GAAATGCAAA TCTTTGCCGG 
    GCTATTGTGA AAGCTGGTGC CAGGCTTGGA GTAAATAACA GCCAAGGAAT CAATATATTT 
    AACTATCAGG TTGCAACCAA GCAGCTTCTT TTTAGACTTT TAGATATGCT ATCTCAGGAA 
    CCACCTTGGT GTGATGGCTC AAATTGTTAT GAGTGTGCTG CAAAGTTTGG AGTCACAACC 
    AGAAAACACC ACTGCCGCCA TTGTGGACGT CTGCTCTGCT CCAAGTGCTC AACCAAGGAG 
    ATTCCCATTA TAAAGTTTGA TCTGAACAAA CCTGTTCGGG TTTGTGACAT CTGCTTTGAT 
    GTGCTGACTC TGGGTGGAGT TTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072887.1
    CDS142..3648
    Translation

    Target ORF information:

    RefSeq Version NM_001079419.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat and FYVE domain containing 1 (ankfy1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001079419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGGAAG AGGAGGTGGG AAAGCTGCAG AAGCATCTAG CGCTTCTCCG TCAGGAGTAT 
    GTCAAGTTGC AGCAAAAGCT GGCAGAGACT GAGAAAAAAT GCACTCTCCT AGCAGCCCAG 
    GCTAACAAAG AAGCCTCCAG CGACTCTTTC ATCAGCCGCC TCCTCTCTAT CGTGGCACAA 
    CTCTTTCAGC AGGAGCAGTA TAGTGATCTG ATGATAAAAG TTGGAGACAA ACACATAAAT 
    GCCCATAAAT TTGTTCTTGC TGCAAGAAGT GAGGTTTGGA GTCCAGCCAA TTTGGCTTCA 
    ACTAAAGAAC TTGACCTGTC AGACTCTAAT CCTGAGGTAA CTATGGCTAT GCTTCGTTGG 
    GTCTACACAG ATGAGCTGGA TTTAAGGGAA GATGATATAT TCTTAACTGA GTTGATGAAG 
    CTAGCTAATC GTTTCCAACT TCAACTACTT AGGGAAAGGT GTGAAAAGGG AGTTATGTCT 
    CTGGTGAATG TGAGGAACTG CATACGTTTT TATCAGACTG CTGAGGAGCT AAATGCCAAT 
    GCTTTGTTAA ACTACTGTGC TGAGATTATT GCAAGTCACT GGGATGACCT AAGAAAAGAG 
    GACTTCAGCT CAATGAGTGC TCAGCTACTA TATAAAATGA TTAAATCAAA GACACAGTTC 
    CCTTTGCACA AAGCAATAAA GTTGGAAAGA GAAGATGTAG TTTTCTTGTA CCTAATAGAA 
    ATGGATTCTC AGTTGCCTGG AAAACTAAAT GAACTGGACC AAAATGGAGA CCTTTCCTTG 
    GATTTAGCTC TTTCCCGGAG ACTGGAAAGC ATAGCAACTA CATTGGTTAA TAACAAAGCT 
    GATGTTGACA TGATGGACAA AAAGGGTTGC AGTTTATTAC ATAAAGCCAT TTGTAGAGAC 
    GATAAATTTG CTGCAAACTT TCTAATAAAA AATGGTGCTC TTGTAAATGC TGCCACTGTT 
    GGAGAGCAAC AGACTCCTCT GCATTTGGTA GCTTCTTGCA CAGTAAAGGA CCGGTCTGTG 
    GAAGTGATGT CCGATATAGC ACAAATTGCT GAAGCACTTT TAAAAGCAGG AGCAAATCCT 
    AACATGCAAG ACAGCAAAGG AAGGAGTCCT CTGCACGCTG CTGTTCTTGC TAGGAATGAG 
    TATGTCTTCA ACCAACTCCT TCTGTGCAAA CAATTAGATC TAGAGCTGAA GGATCATGAG 
    GGAAGCACAG CTCTGTGGCT TGCTGTTCAG TATATCACAG TGTCTTCTGA CCAGTCTGTA 
    AATCCTTTTG ATGATGTCCC TGTTGTAAAT GGCACCTCAT TTGATGAAAA CAGCTTTGCT 
    GCCAGACTTA TTAAACAGGG CAGCAACACA GATGCACCAG ACACTGTAAC AGGCAACTGC 
    TTGTTACAAA GAGCTGCTGC TGCAGGGAAT GAAGCTGCTT CCCTGTTCTT GGCAACCCAT 
    GGGGCAAAAG TCAATCATAA AAATAAATGG GGTGAATCGC CTTTACATAC TGCATGTCGG 
    CATGGACTTT CTAACCTTAC AGCAGAGCTT TTACAACAAG GAGCCAACCC AAATATTCAG 
    ACTCAAGAAC CTATTACTTC TCCAAGAGGA CCTCCAGCAA ACAGTCTGGC AGACAGCACT 
    TACTTACAAA CACCTCTGCA CATGGCAATC GCATATAACC ATCCTGATGT TGTTTCTGTC 
    ATATTGGAAC AAAAAGCTAA TGCTCTTCAT GCAACAAATA ATCTACAGAT CATACCTGAC 
    TTTAGTCTCA AGGACTCCAG AGAACAGACA GTGTTAGGAC TGGCACTATG GACAGGCATG 
    CACACCATTG CCGCACAACT CCTGGGCTCT GGAGCATCCA TCAATGATAC AATGTCAGAT 
    GGACAGACAC TTCTACACAT GGCAATACAA AGACAAGACA GCAAGAGTGC CCTGTTCCTG 
    CTAGAACACC AAGCTGATAT CAATGTCCGA ACCCGGGATG GCGAGACAGC ACTTCAGCTT 
    GCCATTAAAA ATCAGCTTCC TTTGGTGGTG GATGCCATTT GTACAAGAGG CGCTGATATG 
    TCTGTTACTG ATGAACAAGG GAATCCCCCT CTGTGGCTTG CACTAGAGAA TGAGCAGGAG 
    GATATTGCCT CAACTCTGGT AAGACATGGA TGTGATGCTA CATTCTGGGG GCCAGGGCCA 
    AGTGGATGCC AACAGACGCT ACTTCATAGA GCAATTGATG AAAACAAAGA GCAAATTGCT 
    TGTTTCATTA TTCGCAGTGG CTGTGATGTA AATAGCACTA GAAAGCCTGG ACCAAATGGA 
    GAAGGAGAAG AAGAGGCCAG AGATGGGCAA ACCCCCTTGC ATTTAGCAGC TTCTTGGGGA 
    CTGGAGGAGG TAGTGCAGTG CCTTTTGGAG TTTGGTGCCA ATGTAAATAC TCAGGATGCA 
    GAGGGGAGAA CTCCAATACA CGTTGCTATA AGCAACCAGC ATCGTGTCAT TATCCAGTTA 
    TTGATCTCAC ACCCTGACAT CCGGCTGAAT GTTCGTGACA GGCAGGGTAT GACCCCATTC 
    GCATGTGCCA TGACTTTCAA AAATAACAAG GCCGCCGAGG CAATTCTGAA ACGGGAACCA 
    GGAGCCGCTG AGCAGGTGGA TAACAAAGGG AGGAATTTCC TGCATGTTGC AGTTCAGAAT 
    TCAGATATTG AGAGTGTCTT GTTCTTGATA AGTGTTCAGG CTAACGTTAA TTCAAGAGTC 
    CAAGATGCCT CCAAGCTAAC CCCACTGCAT CTAGCTGTGC AAGCAGGATC AGAGATTATT 
    GTCCGGAACC TGCTTCTTGC AGGTGCAAAG GTGAATGAAC TTACCAAGCA TCGTCAGACT 
    GCCCTTCACC TAGCAGCACA ACAGGACCTT CCAGTTATTT GTTCTGTTCT CCTTGAGAAT 
    GGTGTTGACT TTAGTGCTGC AGATGAGAAT GGAAATAATG CTCTGCATCT GGCAATAATG 
    CATGGGCGCT TGAACAATAT ACGCTGCCTT TTAACTGAAT GCAATGTGGA TGCAGAGGCT 
    TTAAACTTAA GAGGTCAGTC CCCAATGCAT ATTTTGGGGC AATATGGAAA GGAAAATGCT 
    GCTGCCATCT CTGAACTCTT TCTGGAATGT ATGCCACAGT ATCCTCTTGA TAAACCAGAT 
    GCAGAAGGAA ACACTGTGCT GCTACTAGCG TATATGAAGG GAAATGCAAA TCTTTGCCGG 
    GCTATTGTGA AAGCTGGTGC CAGGCTTGGA GTAAATAACA GCCAAGGAAT CAATATATTT 
    AACTATCAGG TTGCAACCAA GCAGCTTCTT TTTAGACTTT TAGATATGCT ATCTCAGGAA 
    CCACCTTGGT GTGATGGCTC AAATTGTTAT GAGTGTGCTG CAAAGTTTGG AGTCACAACC 
    AGAAAACACC ACTGCCGCCA TTGTGGACGT CTGCTCTGCT CCAAGTGCTC AACCAAGGAG 
    ATTCCCATTA TAAAGTTTGA TCTGAACAAA CCTGTTCGGG TTTGTGACAT CTGCTTTGAT 
    GTGCTGACTC TGGGTGGAGT TTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ankfy1 ( XM_012957332.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812786.1 Xenopus tropicalis ankyrin repeat and FYVE domain containing 1 (ankfy1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa29721
    Clone ID Related Accession (Same CDS sequence) XM_012957332.1
    Accession Version XM_012957332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rabankyrin-5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTATGC TTCGTTGGGT CTACACAGAT GAGCTGGATT TAAGGGAAGA TGATATATTC 
    TTAACTGAGT TGATGAAGCT AGCTAATCGT TTCCAACTTC AACTACTTAG GGAAAGGTGT 
    GAAAAGGGAG TTATGTCTCT GGTGAATGTG AGGAACTGCA TACGTTTTTA TCAGACTGCT 
    GAGGAGCTAA ATGCCAATGC TTTGTTAAAC TACTGTGCTG AGATTATTGC AAGTCACTGG 
    GATGACCTAA GAAAAGAGGA CTTCAGCTCA ATGAGTGCTC AGCTACTATA TAAAATGATT 
    AAATCAAAGA CACAGTTCCC TTTGCACAAA GCAATAAAGT TGGAAAGAGA AGATGTAGTT 
    TTCTTGTACC TAATAGAAAT GGATTCTCAG TTGCCTGGAA AACTAAATGA ACTGGACCAA 
    AATGGAGACC TTTCCTTGGA TTTAGCTCTT TCCCGGAGAC TGGAAAGCAT AGCAACTACA 
    TTGGTTAATA ACAAAGCTGA TGTTGACATG ATGGACAAAA AGGGTTGCAG TTTATTACAT 
    AAAGCCATTT GTAGAGACGA TAAATTTGCT GCAAACTTTC TAATAAAAAA TGGTGCTCTT 
    GTAAATGCTG CCACTGTTGG AGAGCAACAG ACTCCTCTGC ATTTGGTAGC TTCTTGCACA 
    GTAAAGGACC GGTCTGTGGA AGTGATGTCC GATATAGCAC AAATTGCTGA AGCACTTTTA 
    AAAGCAGGAG CAAATCCTAA CATGCAAGAC AGCAAAGGAA GGAGTCCTCT GCACGCTGCT 
    GTTCTTGCTA GGAATGAGTA TGTCTTCAAC CAACTCCTTC TGTGCAAACA ATTAGATCTA 
    GAGCTGAAGG ATCATGAGGG AAGCACAGCT CTGTGGCTTG CTGTTCAGTA TATCACAGTG 
    TCTTCTGACC AGTCTGTAAA TCCTTTTGAT GATGTCCCTG TTGTAAATGG CACCTCATTT 
    GATGAAAACA GCTTTGCTGC CAGACTTATT AAACAGGGCA GCAACACAGA TGCACCAGAC 
    ACTGTAACAG GCAACTGCTT GTTACAAAGA GCTGCTGCTG CAGGGAATGA AGCTGCTTCC 
    CTGTTCTTGG CAACCCATGG GGCAAAAGTC AATCATAAAA ATAAATGGGG TGAATCGCCT 
    TTACATACTG CATGTCGGCA TGGACTTTCT AACCTTACAG CAGAGCTTTT ACAACAAGGA 
    GCCAACCCAA ATATTCAGAC TCAAGAACCT ATTACTTCTC CAAGAGGACC TCCAGCAAAC 
    AGTCTGGCAG ACAGCACTTA CTTACAAACA CCTCTGCACA TGGCAATCGC ATATAACCAT 
    CCTGATGTTG TTTCTGTCAT ATTGGAACAA AAAGCTAATG CTCTTCATGC AACAAATAAT 
    CTACAGATCA TACCTGACTT TAGTCTCAAG GACTCCAGAG AACAGACAGT GTTAGGACTG 
    GCACTATGGA CAGGCATGCA CACCATTGCC GCACAACTCC TGGGCTCTGG AGCATCCATC 
    AATGATACAA TGTCAGATGG ACAGACACTT CTACACATGG CAATACAAAG ACAAGACAGC 
    AAGAGTGCCC TGTTCCTGCT AGAACACCAA GCTGATATCA ATGTCCGAAC CCGGGATGGC 
    GAGACAGCAC TTCAGCTTGC CATTAAAAAT CAGCTTCCTT TGGTGGTGGA TGCCATTTGT 
    ACAAGAGGCG CTGATATGTC TGTTACTGAT GAACAAGGGA ATCCCCCTCT GTGGCTTGCA 
    CTAGAGAATG AGCAGGAGGA TATTGCCTCA ACTCTGGTAA GACATGGATG TGATGCTACA 
    TTCTGGGGGC CAGGGCCAAG TGGATGCCAA CAGACGCTAC TTCATAGAGC AATTGATGAA 
    AACAAAGAGC AAATTGCTTG TTTCATTATT CGCAGTGGCT GTGATGTAAA TAGCACTAGA 
    AAGCCTGGAC CAAATGGAGA AGGAGAAGAA GAGGCCAGAG ATGGGCAAAC CCCCTTGCAT 
    TTAGCAGCTT CTTGGGGACT GGAGGAGGTA GTGCAGTGCC TTTTGGAGTT TGGTGCCAAT 
    GTAAATACTC AGGATGCAGA GGGGAGAACT CCAATACACG TTGCTATAAG CAACCAGCAT 
    CGTGTCATTA TCCAGTTATT GATCTCACAC CCTGACATCC GGCTGAATGT TCGTGACAGG 
    CAGGGTATGA CCCCATTCGC ATGTGCCATG ACTTTCAAAA ATAACAAGGC CGCCGAGGCA 
    ATTCTGAAAC GGGAACCAGG AGCCGCTGAG CAGGTGGATA ACAAAGGGAG GAATTTCCTG 
    CATGTTGCAG TTCAGAATTC AGATATTGAG AGTGTCTTGT TCTTGATAAG TGTTCAGGCT 
    AACGTTAATT CAAGAGTCCA AGATGCCTCC AAGCTAACCC CACTGCATCT AGCTGTGCAA 
    GCAGGATCAG AGATTATTGT CCGGAACCTG CTTCTTGCAG GTGCAAAGGT GAATGAACTT 
    ACCAAGCATC GTCAGACTGC CCTTCACCTA GCAGCACAAC AGGACCTTCC AGTTATTTGT 
    TCTGTTCTCC TTGAGAATGG TGTTGACTTT AGTGCTGCAG ATGAGAATGG AAATAATGCT 
    CTGCATCTGG CAATAATGCA TGGGCGCTTG AACAATATAC GCTGCCTTTT AACTGAATGC 
    AATGTGGATG CAGAGGCTTT AAACTTAAGA GGTCAGTCCC CAATGCATAT TTTGGGGCAA 
    TATGGAAAGG AAAATGCTGC TGCCATCTCT GAACTCTTTC TGGAATGTAT GCCACAGTAT 
    CCTCTTGATA AACCAGATGC AGAAGGAAAC ACTGTGCTGC TACTAGCGTA TATGAAGGGA 
    AATGCAAATC TTTGCCGGGC TATTGTGAAA GCTGGTGCCA GGCTTGGAGT AAATAACAGC 
    CAAGGAATCA ATATATTTAA CTATCAGGTT GCAACCAAGC AGCTTCTTTT TAGACTTTTA 
    GATATGCTAT CTCAGGAACC ACCTTGGTGT GATGGCTCAA ATTGTTATGA GTGTGCTGCA 
    AAGTTTGGAG TCACAACCAG AAAACACCAC TGCCGCCATT GTGGACGTCT GCTCTGCTCC 
    AAGTGCTCAA CCAAGGAGAT TCCCATTATA AAGTTTGATC TGAACAAACC TGTTCGGGTT 
    TGTGACATCT GCTTTGATGT GCTGACTCTG GGTGGAGTTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812786.1
    CDS136..3300
    Translation

    Target ORF information:

    RefSeq Version XM_012957332.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat and FYVE domain containing 1 (ankfy1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012957332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCTATGC TTCGTTGGGT CTACACAGAT GAGCTGGATT TAAGGGAAGA TGATATATTC 
    TTAACTGAGT TGATGAAGCT AGCTAATCGT TTCCAACTTC AACTACTTAG GGAAAGGTGT 
    GAAAAGGGAG TTATGTCTCT GGTGAATGTG AGGAACTGCA TACGTTTTTA TCAGACTGCT 
    GAGGAGCTAA ATGCCAATGC TTTGTTAAAC TACTGTGCTG AGATTATTGC AAGTCACTGG 
    GATGACCTAA GAAAAGAGGA CTTCAGCTCA ATGAGTGCTC AGCTACTATA TAAAATGATT 
    AAATCAAAGA CACAGTTCCC TTTGCACAAA GCAATAAAGT TGGAAAGAGA AGATGTAGTT 
    TTCTTGTACC TAATAGAAAT GGATTCTCAG TTGCCTGGAA AACTAAATGA ACTGGACCAA 
    AATGGAGACC TTTCCTTGGA TTTAGCTCTT TCCCGGAGAC TGGAAAGCAT AGCAACTACA 
    TTGGTTAATA ACAAAGCTGA TGTTGACATG ATGGACAAAA AGGGTTGCAG TTTATTACAT 
    AAAGCCATTT GTAGAGACGA TAAATTTGCT GCAAACTTTC TAATAAAAAA TGGTGCTCTT 
    GTAAATGCTG CCACTGTTGG AGAGCAACAG ACTCCTCTGC ATTTGGTAGC TTCTTGCACA 
    GTAAAGGACC GGTCTGTGGA AGTGATGTCC GATATAGCAC AAATTGCTGA AGCACTTTTA 
    AAAGCAGGAG CAAATCCTAA CATGCAAGAC AGCAAAGGAA GGAGTCCTCT GCACGCTGCT 
    GTTCTTGCTA GGAATGAGTA TGTCTTCAAC CAACTCCTTC TGTGCAAACA ATTAGATCTA 
    GAGCTGAAGG ATCATGAGGG AAGCACAGCT CTGTGGCTTG CTGTTCAGTA TATCACAGTG 
    TCTTCTGACC AGTCTGTAAA TCCTTTTGAT GATGTCCCTG TTGTAAATGG CACCTCATTT 
    GATGAAAACA GCTTTGCTGC CAGACTTATT AAACAGGGCA GCAACACAGA TGCACCAGAC 
    ACTGTAACAG GCAACTGCTT GTTACAAAGA GCTGCTGCTG CAGGGAATGA AGCTGCTTCC 
    CTGTTCTTGG CAACCCATGG GGCAAAAGTC AATCATAAAA ATAAATGGGG TGAATCGCCT 
    TTACATACTG CATGTCGGCA TGGACTTTCT AACCTTACAG CAGAGCTTTT ACAACAAGGA 
    GCCAACCCAA ATATTCAGAC TCAAGAACCT ATTACTTCTC CAAGAGGACC TCCAGCAAAC 
    AGTCTGGCAG ACAGCACTTA CTTACAAACA CCTCTGCACA TGGCAATCGC ATATAACCAT 
    CCTGATGTTG TTTCTGTCAT ATTGGAACAA AAAGCTAATG CTCTTCATGC AACAAATAAT 
    CTACAGATCA TACCTGACTT TAGTCTCAAG GACTCCAGAG AACAGACAGT GTTAGGACTG 
    GCACTATGGA CAGGCATGCA CACCATTGCC GCACAACTCC TGGGCTCTGG AGCATCCATC 
    AATGATACAA TGTCAGATGG ACAGACACTT CTACACATGG CAATACAAAG ACAAGACAGC 
    AAGAGTGCCC TGTTCCTGCT AGAACACCAA GCTGATATCA ATGTCCGAAC CCGGGATGGC 
    GAGACAGCAC TTCAGCTTGC CATTAAAAAT CAGCTTCCTT TGGTGGTGGA TGCCATTTGT 
    ACAAGAGGCG CTGATATGTC TGTTACTGAT GAACAAGGGA ATCCCCCTCT GTGGCTTGCA 
    CTAGAGAATG AGCAGGAGGA TATTGCCTCA ACTCTGGTAA GACATGGATG TGATGCTACA 
    TTCTGGGGGC CAGGGCCAAG TGGATGCCAA CAGACGCTAC TTCATAGAGC AATTGATGAA 
    AACAAAGAGC AAATTGCTTG TTTCATTATT CGCAGTGGCT GTGATGTAAA TAGCACTAGA 
    AAGCCTGGAC CAAATGGAGA AGGAGAAGAA GAGGCCAGAG ATGGGCAAAC CCCCTTGCAT 
    TTAGCAGCTT CTTGGGGACT GGAGGAGGTA GTGCAGTGCC TTTTGGAGTT TGGTGCCAAT 
    GTAAATACTC AGGATGCAGA GGGGAGAACT CCAATACACG TTGCTATAAG CAACCAGCAT 
    CGTGTCATTA TCCAGTTATT GATCTCACAC CCTGACATCC GGCTGAATGT TCGTGACAGG 
    CAGGGTATGA CCCCATTCGC ATGTGCCATG ACTTTCAAAA ATAACAAGGC CGCCGAGGCA 
    ATTCTGAAAC GGGAACCAGG AGCCGCTGAG CAGGTGGATA ACAAAGGGAG GAATTTCCTG 
    CATGTTGCAG TTCAGAATTC AGATATTGAG AGTGTCTTGT TCTTGATAAG TGTTCAGGCT 
    AACGTTAATT CAAGAGTCCA AGATGCCTCC AAGCTAACCC CACTGCATCT AGCTGTGCAA 
    GCAGGATCAG AGATTATTGT CCGGAACCTG CTTCTTGCAG GTGCAAAGGT GAATGAACTT 
    ACCAAGCATC GTCAGACTGC CCTTCACCTA GCAGCACAAC AGGACCTTCC AGTTATTTGT 
    TCTGTTCTCC TTGAGAATGG TGTTGACTTT AGTGCTGCAG ATGAGAATGG AAATAATGCT 
    CTGCATCTGG CAATAATGCA TGGGCGCTTG AACAATATAC GCTGCCTTTT AACTGAATGC 
    AATGTGGATG CAGAGGCTTT AAACTTAAGA GGTCAGTCCC CAATGCATAT TTTGGGGCAA 
    TATGGAAAGG AAAATGCTGC TGCCATCTCT GAACTCTTTC TGGAATGTAT GCCACAGTAT 
    CCTCTTGATA AACCAGATGC AGAAGGAAAC ACTGTGCTGC TACTAGCGTA TATGAAGGGA 
    AATGCAAATC TTTGCCGGGC TATTGTGAAA GCTGGTGCCA GGCTTGGAGT AAATAACAGC 
    CAAGGAATCA ATATATTTAA CTATCAGGTT GCAACCAAGC AGCTTCTTTT TAGACTTTTA 
    GATATGCTAT CTCAGGAACC ACCTTGGTGT GATGGCTCAA ATTGTTATGA GTGTGCTGCA 
    AAGTTTGGAG TCACAACCAG AAAACACCAC TGCCGCCATT GTGGACGTCT GCTCTGCTCC 
    AAGTGCTCAA CCAAGGAGAT TCCCATTATA AAGTTTGATC TGAACAAACC TGTTCGGGTT 
    TGTGACATCT GCTTTGATGT GCTGACTCTG GGTGGAGTTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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