ralgapa1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ralgapa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ralgapa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa22307 NM_001079003.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa47301 NM_001375315.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa63775 XM_031890553.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63776 XM_031890554.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63777 XM_031890555.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63778 XM_031890556.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63779 XM_031890557.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa63780 XM_031890558.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa63781 XM_031890559.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa63782 XM_031890560.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa63783 XM_031890561.1
Latest version!
Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa22307
    Clone ID Related Accession (Same CDS sequence) NM_001079003.1
    Accession Version NM_001079003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-27
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121886.1. ##Evidence-Data-START## Transcript exon combination :: BC121886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGTTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGATAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAAACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGGTGTGGT GTCTGGTACA GATCATCTCA CCCCCCAGGC TTTGGACCCC
    CCCTCTTCTA ACACACAGTC TCAGGAAGCA CCCATTACGC AGGTCGATGG CAGCATATAT
    GATCATCTCT GCCAGCTTGC TGGCTCAGCT GAATTAAGAG ATGGAATAAA GGAACAGGGC
    TTGTACGAGG AATTAGAGGG TGAAGGTCTG TTGCCACGTT TGAGAGCCTA TTTAAGGGAG
    CAGGAAGTTC TAGAAGTACT GAAAGACAGG ACGGGCAACT ACCTGTTTCA TCAAGACAGC
    GAGCATTCTG AACATGTAGA TTGTTATAAT AAAGGGGAAG TCAGTTCATT CATTCCTGAT
    TTATACAGTC AAGTAGTTTC TTCAGAGCAG AATGATAACT GTTTAGGTGG GGTGGTTAAT
    ATAGCCAAGA AAGCTGGTTG CATTAGGATG AGCAACAGGT CACGAGAAGT CCATGTAAGG
    ATACAGGTTG ATGAAGAAGA AAGGGAGAAT GATTATGTTG CCCAGGAGCA AGGTGAGCGA
    AAGAGGGGCC TCTATAGGCA ACAAGCAGCT GAGGGAGAGG AAACCTCTGA CTTGGCAACT
    TCAAAGTTTC ACCACTCCTC TGGCACTGAA CATGGGGCCA ACATAAAGAA TCTTTGTGAG
    TCTGTAACAC ACAAGGCTAA AGCAATGCGC CGGAAACGCA GCAGTGTTCA GGTTACCTTC
    CGTCCAGCCA CTGAGGCTGT GCTTTTCTAC AACTCTATGG AAAACAAAGA TGCCCCTTGG
    AAAGTGAGGC TGCGTAAATT AAGTGGTTTA AGCACTAACC CCTCTATAGC TGTAGAGCCA
    GGAAAATCCA CTGCCTATGC TGCACTTGGT GCAGTCAGAG TAGCATCAAG TGTTAGCCAG
    CCAGGTAATG ACCACCATGA TTTACCTTAC CCTCCCCCAC AAATCCAAAA GGAATGTGCC
    CGAATAAGGC AGAGATCCTG CAGTCAGGAG AGAGCCCCCT CTCATGCGTC ATTGGGTGGC
    GTATATAAAA CTGTTGTCCA TGCTCTCTCA AAACCTAAGG CCAGTGTGTC CCTGCAGAAG
    CAACAAAAGA TGGCTACCGA GGCTCCACTG AAGGACCTGT ACGGTCATGT TATGGGCTAT
    TTTGGAAGGA AGGCAGCAGG TGAGATGGCA CGCTCTGGGA TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072471.1
    CDS186..3770
    Translation

    Target ORF information:

    RefSeq Version NM_001079003.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGTTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGATAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAAACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGGTGTGGT GTCTGGTACA GATCATCTCA CCCCCCAGGC TTTGGACCCC
    CCCTCTTCTA ACACACAGTC TCAGGAAGCA CCCATTACGC AGGTCGATGG CAGCATATAT
    GATCATCTCT GCCAGCTTGC TGGCTCAGCT GAATTAAGAG ATGGAATAAA GGAACAGGGC
    TTGTACGAGG AATTAGAGGG TGAAGGTCTG TTGCCACGTT TGAGAGCCTA TTTAAGGGAG
    CAGGAAGTTC TAGAAGTACT GAAAGACAGG ACGGGCAACT ACCTGTTTCA TCAAGACAGC
    GAGCATTCTG AACATGTAGA TTGTTATAAT AAAGGGGAAG TCAGTTCATT CATTCCTGAT
    TTATACAGTC AAGTAGTTTC TTCAGAGCAG AATGATAACT GTTTAGGTGG GGTGGTTAAT
    ATAGCCAAGA AAGCTGGTTG CATTAGGATG AGCAACAGGT CACGAGAAGT CCATGTAAGG
    ATACAGGTTG ATGAAGAAGA AAGGGAGAAT GATTATGTTG CCCAGGAGCA AGGTGAGCGA
    AAGAGGGGCC TCTATAGGCA ACAAGCAGCT GAGGGAGAGG AAACCTCTGA CTTGGCAACT
    TCAAAGTTTC ACCACTCCTC TGGCACTGAA CATGGGGCCA ACATAAAGAA TCTTTGTGAG
    TCTGTAACAC ACAAGGCTAA AGCAATGCGC CGGAAACGCA GCAGTGTTCA GGTTACCTTC
    CGTCCAGCCA CTGAGGCTGT GCTTTTCTAC AACTCTATGG AAAACAAAGA TGCCCCTTGG
    AAAGTGAGGC TGCGTAAATT AAGTGGTTTA AGCACTAACC CCTCTATAGC TGTAGAGCCA
    GGAAAATCCA CTGCCTATGC TGCACTTGGT GCAGTCAGAG TAGCATCAAG TGTTAGCCAG
    CCAGGTAATG ACCACCATGA TTTACCTTAC CCTCCCCCAC AAATCCAAAA GGAATGTGCC
    CGAATAAGGC AGAGATCCTG CAGTCAGGAG AGAGCCCCCT CTCATGCGTC ATTGGGTGGC
    GTATATAAAA CTGTTGTCCA TGCTCTCTCA AAACCTAAGG CCAGTGTGTC CCTGCAGAAG
    CAACAAAAGA TGGCTACCGA GGCTCCACTG AAGGACCTGT ACGGTCATGT TATGGGCTAT
    TTTGGAAGGA AGGCAGCAGG TGAGATGGCA CGCTCTGGGA TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47301
    Clone ID Related Accession (Same CDS sequence) NM_001375315.1
    Accession Version NM_001375315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03032465.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGCTACTAA AGAAGATTTA GCTCCCCAAG TCAATGATGT TGCCGGTGCT
    GAAATTGCCA CTAATGCCAA TATCCCAGAC CTGATGGATG AGTTTATAGC TGAGAGACTG
    CGATCAGGCA CCAGTGTGAC TATCACACGT AGGGGCAGCA GTCCTGGTAG TCTTGAGGTT
    CCCAAAGATT TACCTGATAT GCTTAATAAG CATAGCCAGA TGCGCCCAGC TGATGACCCT
    GGTGTCCCTT CAGAGTGGAC CTCTCCGGCA AGCGCAGCCA GCAGTGACCT TCTTAGCTCA
    GACAGCCATT CGGACTCTTT TAATGCCTTC CAGTATGATG CAAGGAAGTT TGAAGGTTTT
    CCATTTGGAG GAGATTCAAC AGTCCCCATC TGTATTGAAG CAGATGCTGG CTCTACACAT
    CTTAGCGTTG AGGAACCAGA AGTTGGAAGT CTTACAGCAC TGCATATTGA CTCTGAGACA
    AGCAGCTTCA ATCAGCAGAT TATGTGCACG GAGGCAGTGG CTGTAACAGG GTCAGAAAGT
    GTTTCCCCTG CTTTGTCTGC TCATGGGTCG AGATCAGAGA CTCCCTCTCC TGCAGCTCTA
    AACTCAGATC AGGCAGAGCT ACACGACAAG CTTCAGCATT CGGTTTTACA AACTCCCGAT
    GATATAGAGC CTTGTGACTT TCCTTTAGAA AGCTGCAGTG TTATGGCTGG AGGAACACTT
    ACTGGATGGC ACGCGGACGT TTCCACAGTC ATGTGGAGAA GAATGCTTGG GATTCTTGGG
    GATGTCAATA ACATTGCCAA TCCTGAAATT CATGCCCAAG TTTTTGATTA CTTGTGTGAA
    CTCTGGCAGA ACCTGGCTAA GATTAGAGAT AATTTGGGAA TATCCTTGGA TAATCAGTCT
    TCTCCCCGAC CTCCTGTTCT TATCCCACCC CTGAGGATTC TCACTCCCTG GCTTTTTAAG
    GCTACGATGC TGCCTGAAAA ATACAAGCAG GGCAAACTCC ATGCTTACAA ACTGATTTGC
    AAGATAATGA AGAGAAGACA GGACGTATCT CCAAACAGGG ACTTCTTAAC GCACTTCTAT
    AACATTATGC ACTGTGGTCT TCTTCACAAC GATCAGGACA TTGTGAATAC TATAATCAAG
    CACTGTTCAC CTCAATTCTT TGCCCTTGGC CTTCCTGGTG CCACACTCCT TATTATGGAT
    TTTATTGTAG CTGCTGGGAG AGTTGCTTCC TCGTCATCCC TTAATGCTCC ACGTGTGGAA
    GCTCAAGTTC TTCTGGGATC GCTTGTCTGT TTCCCCAACC TCTACCAGGA ATTGCCTGCT
    TTGCACCCAA ATAGCTCTGA CATAGTAATG TCACATTTTA CAGATGTCAA GGAACTCACA
    ATTAAAACTG TACTAAATTC AGCCAGAGAT GAACCGTCTG GCCCTGCAAG ATGTGTAGCA
    TTGTGCAGTT TGGGAATATG GATGTGTGAG GAGCTTGTCA ATCAAACACA TCATCCACAA
    ATCAAAGAAG CACTCAATGT CATCTGTGTC TCCTTAAAAT TTCCCAATAA AGTGGTGGCC
    CAGGTCGCCT GCAGTGTGCT AAATGTGCTG CTCAATTATG TTCAGCAGCT TCAGATTTAC
    CATTCAGACT ACCCACTTAA AATCATTGAG ATCCTGCTAG CGACAGTGAC ATATCTGCTG
    CCAAGTGCTG AGACCTCATC TTACGAGCAA GACAAGCGGT TGGTGGTCTC TCTGCTTCTT
    TCCCTTTTGG ACTGGATGAT GGCATTACCC CTTGCATTGT TATTGAAACC ACTACGCCAT
    GCTCGGACGG AGAGTGATGG AAGGGAGAAC TCTGTGTTAA ACTGTGCATA TAAGGTTCTA
    CATGACTGTG TCTATGGCTC CCAGCATTTC AGCAATCCCA GGTACTTTCC AATGAGCCTT
    TCAGATCTGG CATCGGAGGA TTATGATCCC TTTATGCAAC TGGAATGCCT GAAGGAACCA
    GAACCTTTAC ATTCCCCAGA TTCTGAGCGC TCCACCAAAC TGCAGCCTGT TACTGAGGTG
    AAAATTCCAA AGCAGCAAGG TCTTATCCCA GTAGCAGCAC GGACAGTAAT CACACATTTG
    GTAAACCACT TGGGCCACTA TCCGATGAGT GGAGACCCCG CAATGTTGAC TAGCCAGATC
    TGCGAGAACC ATGATAATCC ATACTGTGAA AGTTCGGATC TATCTCCTCA ACTCTTCCAC
    AGCCCCAATC TGCAGTTCTT TGCTCTCAAT AATACCACTC TTGTGTCCTG CCTTCAGATA
    CAGTCTGAAG AGAAACTACC TCCTGGTGAG GACCTCTCTT CTGCCAGATC AACTGTACGC
    ATAATTATGC GGGACCTTTC TGGCAAGTAC AGTTGGGATT CCTCTATCTT ATACGGACCT
    CCTCTCCCAT GTGAGGGACA AATGACTCAG TGGTCTTCCC TTCTGGCAAT ATTTTCTCCT
    CCTTCCAATC AAGAGGTTGA ACACCCATCA ACCCAGAGGG AATCTGAGGA CTTTTCTTCT
    CTTTCTTCAA GAAGGCGAGG AAGGGAATCT CTGCCCCTAT GGGACAGCTT ATCAGATGAA
    CAAGATGCTC TTGACTGCCT TTTGCAGTAT CTGGGAGCTA CTGGTCCAGA GTGCCTTCAG
    AGGGCTGGTG TTCCCCTCAA TGTGCCCTCA CCTCCACCTG CTTGCTTCTC TGAGAAGCTG
    GAGAATGAGG TTAGCAATGC AATTCTGAAA CAGAGTGCTG AAGAAAAGGA ATTTGTGGAA
    AAGCATAGCA GTAATCTTCA CATGAGAGCA ATGCCTCAAG ATCAGCCTGT AAACCAAAAA
    CCCCAATCAG CTTTCTACTA CTCCCGGCTG TTGCTTAGTA TCCTTGGCAT GAACTCCTGG
    GAGAAGAGGC GAAACTTCCA CTTGCTTAAG AAGAATGAGA AACTGTTACG TGAGCTGAAA
    AATTTGGACT CCAGGCAGTG CCGCGAAACC CACAAAATTG CAGTTTTCTA CGTTGCAGAC
    GGCCAGGAGG ACAAGCATTC AATCCTATCT AACACCGGTG GCAGTCAGGA CTATGAGGAT
    TTTGTGGCTG GTCTTGGATG GGAGGTCAAT CTTTCCAATC ACTGTGGCTT CCTGGGAGGA
    CTTCAGAGGA ACAAGAGCAC TGGCTTTAGC ACTCCATATT TTGCAACCTC AATAGTGGAG
    GTCATGTTCC ATGTCTCCAC AAGAATGATT TCAGACTCTG ATGATTGTTT AACCAAAAAG
    CTTCGCCACC TTGGGAATGA TGAGGTGCAC ATTGTGTGGT CAGAACACAG TCGGGAATAT
    CGCCGCGGCA TCATACCCAC TGAATTTGGG GACGTCCTAC TTGTAATATA CCCCATGAAG
    AACCACATGT TCAGCATCCA GATTATGAAG AAGCCAGAAA TCCCATTTTT TGGGCCCCTC
    TTCGATGGAG CGATTGTGAA TGGCAAAATC CTCCCAGGAC TGGTTCGTGC AACAGCCATC
    AATGCCAGCA GAGCACTTAA ATCTCTTATC CCCCTGTACC AAAATTTCTT TGAGGAACGG
    ATGCGATACT TGGATTCAAT CACACAGCAG CATTTGGAAC CCACCAGCTT TGAAGACTAC
    GCTGCCCAGG TGTTCTGCCC AGTGCCGCAC AATCCCCTCC AGCTGAATGC ACGGCCTTGC
    CCAACGAAAG AGCAAGATGA GAACGTATCG CCTCCGGGGG ATGGATCAGA ACTGGCTTTT
    TCTTTGTCAC CACGGAGCAG CAAAAGTCGC ATGTCCATAA AGCTCCGGCG TTCCTCTGGC
    TCTGCCAACA AAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362244.1
    CDS277..6474
    Translation

    Target ORF information:

    RefSeq Version NM_001375315.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGCTACTAA AGAAGATTTA GCTCCCCAAG TCAATGATGT TGCCGGTGCT
    GAAATTGCCA CTAATGCCAA TATCCCAGAC CTGATGGATG AGTTTATAGC TGAGAGACTG
    CGATCAGGCA CCAGTGTGAC TATCACACGT AGGGGCAGCA GTCCTGGTAG TCTTGAGGTT
    CCCAAAGATT TACCTGATAT GCTTAATAAG CATAGCCAGA TGCGCCCAGC TGATGACCCT
    GGTGTCCCTT CAGAGTGGAC CTCTCCGGCA AGCGCAGCCA GCAGTGACCT TCTTAGCTCA
    GACAGCCATT CGGACTCTTT TAATGCCTTC CAGTATGATG CAAGGAAGTT TGAAGGTTTT
    CCATTTGGAG GAGATTCAAC AGTCCCCATC TGTATTGAAG CAGATGCTGG CTCTACACAT
    CTTAGCGTTG AGGAACCAGA AGTTGGAAGT CTTACAGCAC TGCATATTGA CTCTGAGACA
    AGCAGCTTCA ATCAGCAGAT TATGTGCACG GAGGCAGTGG CTGTAACAGG GTCAGAAAGT
    GTTTCCCCTG CTTTGTCTGC TCATGGGTCG AGATCAGAGA CTCCCTCTCC TGCAGCTCTA
    AACTCAGATC AGGCAGAGCT ACACGACAAG CTTCAGCATT CGGTTTTACA AACTCCCGAT
    GATATAGAGC CTTGTGACTT TCCTTTAGAA AGCTGCAGTG TTATGGCTGG AGGAACACTT
    ACTGGATGGC ACGCGGACGT TTCCACAGTC ATGTGGAGAA GAATGCTTGG GATTCTTGGG
    GATGTCAATA ACATTGCCAA TCCTGAAATT CATGCCCAAG TTTTTGATTA CTTGTGTGAA
    CTCTGGCAGA ACCTGGCTAA GATTAGAGAT AATTTGGGAA TATCCTTGGA TAATCAGTCT
    TCTCCCCGAC CTCCTGTTCT TATCCCACCC CTGAGGATTC TCACTCCCTG GCTTTTTAAG
    GCTACGATGC TGCCTGAAAA ATACAAGCAG GGCAAACTCC ATGCTTACAA ACTGATTTGC
    AAGATAATGA AGAGAAGACA GGACGTATCT CCAAACAGGG ACTTCTTAAC GCACTTCTAT
    AACATTATGC ACTGTGGTCT TCTTCACAAC GATCAGGACA TTGTGAATAC TATAATCAAG
    CACTGTTCAC CTCAATTCTT TGCCCTTGGC CTTCCTGGTG CCACACTCCT TATTATGGAT
    TTTATTGTAG CTGCTGGGAG AGTTGCTTCC TCGTCATCCC TTAATGCTCC ACGTGTGGAA
    GCTCAAGTTC TTCTGGGATC GCTTGTCTGT TTCCCCAACC TCTACCAGGA ATTGCCTGCT
    TTGCACCCAA ATAGCTCTGA CATAGTAATG TCACATTTTA CAGATGTCAA GGAACTCACA
    ATTAAAACTG TACTAAATTC AGCCAGAGAT GAACCGTCTG GCCCTGCAAG ATGTGTAGCA
    TTGTGCAGTT TGGGAATATG GATGTGTGAG GAGCTTGTCA ATCAAACACA TCATCCACAA
    ATCAAAGAAG CACTCAATGT CATCTGTGTC TCCTTAAAAT TTCCCAATAA AGTGGTGGCC
    CAGGTCGCCT GCAGTGTGCT AAATGTGCTG CTCAATTATG TTCAGCAGCT TCAGATTTAC
    CATTCAGACT ACCCACTTAA AATCATTGAG ATCCTGCTAG CGACAGTGAC ATATCTGCTG
    CCAAGTGCTG AGACCTCATC TTACGAGCAA GACAAGCGGT TGGTGGTCTC TCTGCTTCTT
    TCCCTTTTGG ACTGGATGAT GGCATTACCC CTTGCATTGT TATTGAAACC ACTACGCCAT
    GCTCGGACGG AGAGTGATGG AAGGGAGAAC TCTGTGTTAA ACTGTGCATA TAAGGTTCTA
    CATGACTGTG TCTATGGCTC CCAGCATTTC AGCAATCCCA GGTACTTTCC AATGAGCCTT
    TCAGATCTGG CATCGGAGGA TTATGATCCC TTTATGCAAC TGGAATGCCT GAAGGAACCA
    GAACCTTTAC ATTCCCCAGA TTCTGAGCGC TCCACCAAAC TGCAGCCTGT TACTGAGGTG
    AAAATTCCAA AGCAGCAAGG TCTTATCCCA GTAGCAGCAC GGACAGTAAT CACACATTTG
    GTAAACCACT TGGGCCACTA TCCGATGAGT GGAGACCCCG CAATGTTGAC TAGCCAGATC
    TGCGAGAACC ATGATAATCC ATACTGTGAA AGTTCGGATC TATCTCCTCA ACTCTTCCAC
    AGCCCCAATC TGCAGTTCTT TGCTCTCAAT AATACCACTC TTGTGTCCTG CCTTCAGATA
    CAGTCTGAAG AGAAACTACC TCCTGGTGAG GACCTCTCTT CTGCCAGATC AACTGTACGC
    ATAATTATGC GGGACCTTTC TGGCAAGTAC AGTTGGGATT CCTCTATCTT ATACGGACCT
    CCTCTCCCAT GTGAGGGACA AATGACTCAG TGGTCTTCCC TTCTGGCAAT ATTTTCTCCT
    CCTTCCAATC AAGAGGTTGA ACACCCATCA ACCCAGAGGG AATCTGAGGA CTTTTCTTCT
    CTTTCTTCAA GAAGGCGAGG AAGGGAATCT CTGCCCCTAT GGGACAGCTT ATCAGATGAA
    CAAGATGCTC TTGACTGCCT TTTGCAGTAT CTGGGAGCTA CTGGTCCAGA GTGCCTTCAG
    AGGGCTGGTG TTCCCCTCAA TGTGCCCTCA CCTCCACCTG CTTGCTTCTC TGAGAAGCTG
    GAGAATGAGG TTAGCAATGC AATTCTGAAA CAGAGTGCTG AAGAAAAGGA ATTTGTGGAA
    AAGCATAGCA GTAATCTTCA CATGAGAGCA ATGCCTCAAG ATCAGCCTGT AAACCAAAAA
    CCCCAATCAG CTTTCTACTA CTCCCGGCTG TTGCTTAGTA TCCTTGGCAT GAACTCCTGG
    GAGAAGAGGC GAAACTTCCA CTTGCTTAAG AAGAATGAGA AACTGTTACG TGAGCTGAAA
    AATTTGGACT CCAGGCAGTG CCGCGAAACC CACAAAATTG CAGTTTTCTA CGTTGCAGAC
    GGCCAGGAGG ACAAGCATTC AATCCTATCT AACACCGGTG GCAGTCAGGA CTATGAGGAT
    TTTGTGGCTG GTCTTGGATG GGAGGTCAAT CTTTCCAATC ACTGTGGCTT CCTGGGAGGA
    CTTCAGAGGA ACAAGAGCAC TGGCTTTAGC ACTCCATATT TTGCAACCTC AATAGTGGAG
    GTCATGTTCC ATGTCTCCAC AAGAATGATT TCAGACTCTG ATGATTGTTT AACCAAAAAG
    CTTCGCCACC TTGGGAATGA TGAGGTGCAC ATTGTGTGGT CAGAACACAG TCGGGAATAT
    CGCCGCGGCA TCATACCCAC TGAATTTGGG GACGTCCTAC TTGTAATATA CCCCATGAAG
    AACCACATGT TCAGCATCCA GATTATGAAG AAGCCAGAAA TCCCATTTTT TGGGCCCCTC
    TTCGATGGAG CGATTGTGAA TGGCAAAATC CTCCCAGGAC TGGTTCGTGC AACAGCCATC
    AATGCCAGCA GAGCACTTAA ATCTCTTATC CCCCTGTACC AAAATTTCTT TGAGGAACGG
    ATGCGATACT TGGATTCAAT CACACAGCAG CATTTGGAAC CCACCAGCTT TGAAGACTAC
    GCTGCCCAGG TGTTCTGCCC AGTGCCGCAC AATCCCCTCC AGCTGAATGC ACGGCCTTGC
    CCAACGAAAG AGCAAGATGA GAACGTATCG CCTCCGGGGG ATGGATCAGA ACTGGCTTTT
    TCTTTGTCAC CACGGAGCAG CAAAAGTCGC ATGTCCATAA AGCTCCGGCG TTCCTCTGGC
    TCTGCCAACA AAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63775
    Clone ID Related Accession (Same CDS sequence) XM_031890553.1
    Accession Version XM_031890553.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGCAGGTTTT CCATTTGGAG GAGATTCAAC AGTCCCCATC
    TGTATTGAAG CAGATGCTGG CTCTACACAT CTTAGCGTTG AGGAACCAGA AGTTGGAAGT
    CTTACAGCAC TGCATATTGA CTCTGAGACA AGCAGCTTCA ATCAGCAGAT TATGTGCACG
    GAGGCAGTGG CTGTAACAGG GTCAGAAAGT GTTTCCCCTG CTTTGTCTGC TCATGGGTCG
    AGATCAGAGA CTCCCTCTCC TGCAGCTCTA AACTCAGATC AGGCAGAGCT ACACGACAAG
    CTTCAGCATT CGGTTTTACA AACTCCCGAT GATATAGAGC CTTGTGACTT TCCTTTAGAA
    AGCTGCAGTG TTATGGCTGG AGGAACACTT ACTGGATGGC ACGCGGACGT TTCCACAGTC
    ATGTGGAGAA GAATGCTTGG GATTCTTGGG GATGTCAATA ACATTGCCAA TCCTGAAATT
    CATGCCCAAG TTTTTGATTA CTTGTGTGAA CTCTGGCAGA ACCTGGCTAA GATTAGAGAT
    AATTTGGGAA TATCCTTGGA TAATCAGTCT TCTCCCCGAC CTCCTGTTCT TATCCCACCC
    CTGAGGATTC TCACTCCCTG GCTTTTTAAG GCTACGATGC TGCCTGAAAA ATACAAGCAG
    GGCAAACTCC ATGCTTACAA ACTGATTTGC AAGATAATGA AGAGAAGACA GGACGTATCT
    CCAAACAGGG ACTTCTTAAC GCACTTCTAT AACATTATGC ACTGTGGTCT TCTTCACAAC
    GATCAGGACA TTGTGAATAC TATAATCAAG CACTGTTCAC CTCAATTCTT TGCCCTTGGC
    CTTCCTGGTG CCACACTCCT TATTATGGAT TTTATTGTAG CTGCTGGGAG AGTTGCTTCC
    TCGTCATCCC TTAATGCTCC ACGTGTGGAA GCTCAAGTTC TTCTGGGATC GCTTGTCTGT
    TTCCCCAACC TCTACCAGGA ATTGCCTGCT TTGCACCCAA ATAGCTCTGA CATAGTAATG
    TCACATTTTA CAGATGTCAA GGAACTCACA ATTAAAACTG TACTAAATTC AGCCAGAGAT
    GAACCGTCTG GCCCTGCAAG ATGTGTAGCA TTGTGCAGTT TGGGAATATG GATGTGTGAG
    GAGCTTGTCA ATCAAACACA TCATCCACAA ATCAAAGAAG CACTCAATGT CATCTGTGTC
    TCCTTAAAAT TTCCCAATAA AGTGGTGGCC CAGGTCGCCT GCAGTGTGCT AAATGTGCTG
    CTCAATTATG TTCAGCAGCT TCAGATTTAC CATTCAGACT ACCCACTTAA AATCATTGAG
    ATCCTGCTAG CGACAGTGAC ATATCTGCTG CCAAGTGCTG AGACCTCATC TTACGAGCAA
    GACAAGCGGT TGGTGGTCTC TCTGCTTCTT TCCCTTTTGG ACTGGATGAT GGCATTACCC
    CTTGCATTGT TATTGAAACC ACTACGCCAT GCTCGGACGG AGAGTGATGG AAGGGAGAAC
    TCTGTGTTAA ACTGTGCATA TAAGGTTCTA CATGACTGTG TCTATGGCTC CCAGCATTTC
    AGCAATCCCA GGTACTTTCC AATGAGCCTT TCAGATCTGG CATCGGAGGA TTATGATCCC
    TTTATGCAAC TGGAATGCCT GAAGGAACCA GAACCTTTAC ATTCCCCAGA TTCTGAGCGC
    TCCACCAAAC TGCAGCCTGT TACTGAGGTG AAAATTCCAA AGCAGCAAGG TCTTATTCCA
    GTAGCAGCAC GGACAGTAAT CACACATTTG GTAAACCACT TGGGCCACTA TCCAATGAGT
    GGAGACCCCG CAATGTTGAC TAGCCAGATC TGCGAGAACC ATGATAATCC ATACTGTGAA
    AGTTCGGATC TATCTCCTCA ACTCTTCCAC AGCCCCAATC TGCAGTTCTT TGCTCTCAAT
    AATACCACTC TTGTGTCCTG CCTTCAGATA CAGTCTGAAG AGAAACTACC TCCTGGTGAG
    GACCTCTCTT CTGCCAGATC AACTGTACGC ATAATTATGC GGGACCTTTC TGGCAAGTAC
    AGTTGGGATT CCTCTATATT ATACGGACCT CCTCTCCCAT GTGAGGGACA AATGACTCAG
    TGGTCTTCCC TTCTGGCAAC ATTTTCTCCT CCTTCCAATC AAGAGGTTGA ACACCCATCA
    ACCCAGAGGG AATCTGAGGA CTTTTCTTCT CTTTCTTCAA GAAGGCGAGG AAGGGAATCT
    CTGCCCCTAT GGGACAGCTT ATCAGATGAA CAAGATGCTC TTGACTGCCT TTTGCAGTAT
    CTGGGAGCTA CTGGTCCAGA GTGCCTTCAG AGGGCTGGTG TTCCCCTCAA TGTGCCCTCA
    CCTCCACCTG CTTGTTTCTC TGAGAAGCTG GAGAATGAGG TTAGCAATGC AATTCTGAAA
    CAGAGTGCAG AAGAAAAGGA ATTTGTGGAA AAGCATAGCA GTAATCTTCA CATGAGAGCA
    ATGCCTCAAG ATCAGCCTGT AAACCAAAAA CCCCAATCAG CTTTCTACTA CTCCCGGCTG
    TTGCTTAGTA TCCTTGGCAT GAACTCCTGG GAGAAGAGGC GAAACTTCCA CTTGCTTAAG
    AAGAATGAGA AACTGTTACG TGAGCTGAAA AATTTGGACT CCAGGCAGTG CCGCGAAACC
    CACAAAATTG CAGTTTTCTA CGTTGCAGAC GGCCAGGAGG ACAAGCATTC AATCCTATCT
    AACACCGGTG GCAGTCAGGA CTATGAGGAT TTTGTGGCTG GTCTTGGATG GGAGGTCAAT
    CTTTCCAATC ACTGTGGCTT CCTGGGAGGA CTTCAGAGGA ACAAGAGCAC TGGCTTTAGC
    ACTCCATATT TTGCAACCTC AATAGTGGAG GTCATGTTCC ATGTCTCCAC AAGAATGATT
    TCAGACTCTG ATGATTGTTT AACCAAAAAG CTTCGCCACC TTGGGAATGA TGAGGTGCAC
    ATTGTGTGGT CAGAACACAG TCGGGAATAT CGCCGCGGCA TCATACCCAC TGAATTTGGG
    GACGTCCTAC TTGTAATATA CCCCATGAAG AACCACATGT TCAGCATCCA GATTATGAAG
    AAGCCAGAAA TCCCATTTTT TGGGCCCCTC TTCGATGGAG CGATTGTGAA TGGCAAAATC
    CTCCCAGGAC TGGTTCGTGC AACAGCCATC AATGCCAGCA GAGCACTTAA ATCTCTTATC
    CCCCTGTACC AAAATTTCTT TGAGGAACGG ATGCGATACT TGGATTCAAT CACACAGCAG
    CATTTGGAAC CCACCACCTT TGAAGACTAC GCTGCCCAGG TGTTCTGCCC AGTGCCGCAC
    AATCCCCTCC AGCTGAATGC ACGGCCTTGC CCAACGAAAG AGCAAGATGA GAACGTATCG
    CCTCCGGGGG ATGGATCAGA ACTGGCTTTT TCTTTGTCAC CACGGAGCAG CAAAAGTCGC
    ATGTCCATAA AGCTCCGGCG TTCCTCTGGC TCTGCCAACA AAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746413.1
    CDS260..7747
    Translation

    Target ORF information:

    RefSeq Version XM_031890553.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGCAGGTTTT CCATTTGGAG GAGATTCAAC AGTCCCCATC
    TGTATTGAAG CAGATGCTGG CTCTACACAT CTTAGCGTTG AGGAACCAGA AGTTGGAAGT
    CTTACAGCAC TGCATATTGA CTCTGAGACA AGCAGCTTCA ATCAGCAGAT TATGTGCACG
    GAGGCAGTGG CTGTAACAGG GTCAGAAAGT GTTTCCCCTG CTTTGTCTGC TCATGGGTCG
    AGATCAGAGA CTCCCTCTCC TGCAGCTCTA AACTCAGATC AGGCAGAGCT ACACGACAAG
    CTTCAGCATT CGGTTTTACA AACTCCCGAT GATATAGAGC CTTGTGACTT TCCTTTAGAA
    AGCTGCAGTG TTATGGCTGG AGGAACACTT ACTGGATGGC ACGCGGACGT TTCCACAGTC
    ATGTGGAGAA GAATGCTTGG GATTCTTGGG GATGTCAATA ACATTGCCAA TCCTGAAATT
    CATGCCCAAG TTTTTGATTA CTTGTGTGAA CTCTGGCAGA ACCTGGCTAA GATTAGAGAT
    AATTTGGGAA TATCCTTGGA TAATCAGTCT TCTCCCCGAC CTCCTGTTCT TATCCCACCC
    CTGAGGATTC TCACTCCCTG GCTTTTTAAG GCTACGATGC TGCCTGAAAA ATACAAGCAG
    GGCAAACTCC ATGCTTACAA ACTGATTTGC AAGATAATGA AGAGAAGACA GGACGTATCT
    CCAAACAGGG ACTTCTTAAC GCACTTCTAT AACATTATGC ACTGTGGTCT TCTTCACAAC
    GATCAGGACA TTGTGAATAC TATAATCAAG CACTGTTCAC CTCAATTCTT TGCCCTTGGC
    CTTCCTGGTG CCACACTCCT TATTATGGAT TTTATTGTAG CTGCTGGGAG AGTTGCTTCC
    TCGTCATCCC TTAATGCTCC ACGTGTGGAA GCTCAAGTTC TTCTGGGATC GCTTGTCTGT
    TTCCCCAACC TCTACCAGGA ATTGCCTGCT TTGCACCCAA ATAGCTCTGA CATAGTAATG
    TCACATTTTA CAGATGTCAA GGAACTCACA ATTAAAACTG TACTAAATTC AGCCAGAGAT
    GAACCGTCTG GCCCTGCAAG ATGTGTAGCA TTGTGCAGTT TGGGAATATG GATGTGTGAG
    GAGCTTGTCA ATCAAACACA TCATCCACAA ATCAAAGAAG CACTCAATGT CATCTGTGTC
    TCCTTAAAAT TTCCCAATAA AGTGGTGGCC CAGGTCGCCT GCAGTGTGCT AAATGTGCTG
    CTCAATTATG TTCAGCAGCT TCAGATTTAC CATTCAGACT ACCCACTTAA AATCATTGAG
    ATCCTGCTAG CGACAGTGAC ATATCTGCTG CCAAGTGCTG AGACCTCATC TTACGAGCAA
    GACAAGCGGT TGGTGGTCTC TCTGCTTCTT TCCCTTTTGG ACTGGATGAT GGCATTACCC
    CTTGCATTGT TATTGAAACC ACTACGCCAT GCTCGGACGG AGAGTGATGG AAGGGAGAAC
    TCTGTGTTAA ACTGTGCATA TAAGGTTCTA CATGACTGTG TCTATGGCTC CCAGCATTTC
    AGCAATCCCA GGTACTTTCC AATGAGCCTT TCAGATCTGG CATCGGAGGA TTATGATCCC
    TTTATGCAAC TGGAATGCCT GAAGGAACCA GAACCTTTAC ATTCCCCAGA TTCTGAGCGC
    TCCACCAAAC TGCAGCCTGT TACTGAGGTG AAAATTCCAA AGCAGCAAGG TCTTATTCCA
    GTAGCAGCAC GGACAGTAAT CACACATTTG GTAAACCACT TGGGCCACTA TCCAATGAGT
    GGAGACCCCG CAATGTTGAC TAGCCAGATC TGCGAGAACC ATGATAATCC ATACTGTGAA
    AGTTCGGATC TATCTCCTCA ACTCTTCCAC AGCCCCAATC TGCAGTTCTT TGCTCTCAAT
    AATACCACTC TTGTGTCCTG CCTTCAGATA CAGTCTGAAG AGAAACTACC TCCTGGTGAG
    GACCTCTCTT CTGCCAGATC AACTGTACGC ATAATTATGC GGGACCTTTC TGGCAAGTAC
    AGTTGGGATT CCTCTATATT ATACGGACCT CCTCTCCCAT GTGAGGGACA AATGACTCAG
    TGGTCTTCCC TTCTGGCAAC ATTTTCTCCT CCTTCCAATC AAGAGGTTGA ACACCCATCA
    ACCCAGAGGG AATCTGAGGA CTTTTCTTCT CTTTCTTCAA GAAGGCGAGG AAGGGAATCT
    CTGCCCCTAT GGGACAGCTT ATCAGATGAA CAAGATGCTC TTGACTGCCT TTTGCAGTAT
    CTGGGAGCTA CTGGTCCAGA GTGCCTTCAG AGGGCTGGTG TTCCCCTCAA TGTGCCCTCA
    CCTCCACCTG CTTGTTTCTC TGAGAAGCTG GAGAATGAGG TTAGCAATGC AATTCTGAAA
    CAGAGTGCAG AAGAAAAGGA ATTTGTGGAA AAGCATAGCA GTAATCTTCA CATGAGAGCA
    ATGCCTCAAG ATCAGCCTGT AAACCAAAAA CCCCAATCAG CTTTCTACTA CTCCCGGCTG
    TTGCTTAGTA TCCTTGGCAT GAACTCCTGG GAGAAGAGGC GAAACTTCCA CTTGCTTAAG
    AAGAATGAGA AACTGTTACG TGAGCTGAAA AATTTGGACT CCAGGCAGTG CCGCGAAACC
    CACAAAATTG CAGTTTTCTA CGTTGCAGAC GGCCAGGAGG ACAAGCATTC AATCCTATCT
    AACACCGGTG GCAGTCAGGA CTATGAGGAT TTTGTGGCTG GTCTTGGATG GGAGGTCAAT
    CTTTCCAATC ACTGTGGCTT CCTGGGAGGA CTTCAGAGGA ACAAGAGCAC TGGCTTTAGC
    ACTCCATATT TTGCAACCTC AATAGTGGAG GTCATGTTCC ATGTCTCCAC AAGAATGATT
    TCAGACTCTG ATGATTGTTT AACCAAAAAG CTTCGCCACC TTGGGAATGA TGAGGTGCAC
    ATTGTGTGGT CAGAACACAG TCGGGAATAT CGCCGCGGCA TCATACCCAC TGAATTTGGG
    GACGTCCTAC TTGTAATATA CCCCATGAAG AACCACATGT TCAGCATCCA GATTATGAAG
    AAGCCAGAAA TCCCATTTTT TGGGCCCCTC TTCGATGGAG CGATTGTGAA TGGCAAAATC
    CTCCCAGGAC TGGTTCGTGC AACAGCCATC AATGCCAGCA GAGCACTTAA ATCTCTTATC
    CCCCTGTACC AAAATTTCTT TGAGGAACGG ATGCGATACT TGGATTCAAT CACACAGCAG
    CATTTGGAAC CCACCACCTT TGAAGACTAC GCTGCCCAGG TGTTCTGCCC AGTGCCGCAC
    AATCCCCTCC AGCTGAATGC ACGGCCTTGC CCAACGAAAG AGCAAGATGA GAACGTATCG
    CCTCCGGGGG ATGGATCAGA ACTGGCTTTT TCTTTGTCAC CACGGAGCAG CAAAAGTCGC
    ATGTCCATAA AGCTCCGGCG TTCCTCTGGC TCTGCCAACA AAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63776
    Clone ID Related Accession (Same CDS sequence) XM_031890554.1
    Accession Version XM_031890554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGGTTTTCCA TTTGGAGGAG ATTCAACAGT CCCCATCTGT
    ATTGAAGCAG ATGCTGGCTC TACACATCTT AGCGTTGAGG AACCAGAAGT TGGAAGTCTT
    ACAGCACTGC ATATTGACTC TGAGACAAGC AGCTTCAATC AGCAGATTAT GTGCACGGAG
    GCAGTGGCTG TAACAGGGTC AGAAAGTGTT TCCCCTGCTT TGTCTGCTCA TGGGTCGAGA
    TCAGAGACTC CCTCTCCTGC AGCTCTAAAC TCAGATCAGG CAGAGCTACA CGACAAGCTT
    CAGCATTCGG TTTTACAAAC TCCCGATGAT ATAGAGCCTT GTGACTTTCC TTTAGAAAGC
    TGCAGTGTTA TGGCTGGAGG AACACTTACT GGATGGCACG CGGACGTTTC CACAGTCATG
    TGGAGAAGAA TGCTTGGGAT TCTTGGGGAT GTCAATAACA TTGCCAATCC TGAAATTCAT
    GCCCAAGTTT TTGATTACTT GTGTGAACTC TGGCAGAACC TGGCTAAGAT TAGAGATAAT
    TTGGGAATAT CCTTGGATAA TCAGTCTTCT CCCCGACCTC CTGTTCTTAT CCCACCCCTG
    AGGATTCTCA CTCCCTGGCT TTTTAAGGCT ACGATGCTGC CTGAAAAATA CAAGCAGGGC
    AAACTCCATG CTTACAAACT GATTTGCAAG ATAATGAAGA GAAGACAGGA CGTATCTCCA
    AACAGGGACT TCTTAACGCA CTTCTATAAC ATTATGCACT GTGGTCTTCT TCACAACGAT
    CAGGACATTG TGAATACTAT AATCAAGCAC TGTTCACCTC AATTCTTTGC CCTTGGCCTT
    CCTGGTGCCA CACTCCTTAT TATGGATTTT ATTGTAGCTG CTGGGAGAGT TGCTTCCTCG
    TCATCCCTTA ATGCTCCACG TGTGGAAGCT CAAGTTCTTC TGGGATCGCT TGTCTGTTTC
    CCCAACCTCT ACCAGGAATT GCCTGCTTTG CACCCAAATA GCTCTGACAT AGTAATGTCA
    CATTTTACAG ATGTCAAGGA ACTCACAATT AAAACTGTAC TAAATTCAGC CAGAGATGAA
    CCGTCTGGCC CTGCAAGATG TGTAGCATTG TGCAGTTTGG GAATATGGAT GTGTGAGGAG
    CTTGTCAATC AAACACATCA TCCACAAATC AAAGAAGCAC TCAATGTCAT CTGTGTCTCC
    TTAAAATTTC CCAATAAAGT GGTGGCCCAG GTCGCCTGCA GTGTGCTAAA TGTGCTGCTC
    AATTATGTTC AGCAGCTTCA GATTTACCAT TCAGACTACC CACTTAAAAT CATTGAGATC
    CTGCTAGCGA CAGTGACATA TCTGCTGCCA AGTGCTGAGA CCTCATCTTA CGAGCAAGAC
    AAGCGGTTGG TGGTCTCTCT GCTTCTTTCC CTTTTGGACT GGATGATGGC ATTACCCCTT
    GCATTGTTAT TGAAACCACT ACGCCATGCT CGGACGGAGA GTGATGGAAG GGAGAACTCT
    GTGTTAAACT GTGCATATAA GGTTCTACAT GACTGTGTCT ATGGCTCCCA GCATTTCAGC
    AATCCCAGGT ACTTTCCAAT GAGCCTTTCA GATCTGGCAT CGGAGGATTA TGATCCCTTT
    ATGCAACTGG AATGCCTGAA GGAACCAGAA CCTTTACATT CCCCAGATTC TGAGCGCTCC
    ACCAAACTGC AGCCTGTTAC TGAGGTGAAA ATTCCAAAGC AGCAAGGTCT TATTCCAGTA
    GCAGCACGGA CAGTAATCAC ACATTTGGTA AACCACTTGG GCCACTATCC AATGAGTGGA
    GACCCCGCAA TGTTGACTAG CCAGATCTGC GAGAACCATG ATAATCCATA CTGTGAAAGT
    TCGGATCTAT CTCCTCAACT CTTCCACAGC CCCAATCTGC AGTTCTTTGC TCTCAATAAT
    ACCACTCTTG TGTCCTGCCT TCAGATACAG TCTGAAGAGA AACTACCTCC TGGTGAGGAC
    CTCTCTTCTG CCAGATCAAC TGTACGCATA ATTATGCGGG ACCTTTCTGG CAAGTACAGT
    TGGGATTCCT CTATATTATA CGGACCTCCT CTCCCATGTG AGGGACAAAT GACTCAGTGG
    TCTTCCCTTC TGGCAACATT TTCTCCTCCT TCCAATCAAG AGGTTGAACA CCCATCAACC
    CAGAGGGAAT CTGAGGACTT TTCTTCTCTT TCTTCAAGAA GGCGAGGAAG GGAATCTCTG
    CCCCTATGGG ACAGCTTATC AGATGAACAA GATGCTCTTG ACTGCCTTTT GCAGTATCTG
    GGAGCTACTG GTCCAGAGTG CCTTCAGAGG GCTGGTGTTC CCCTCAATGT GCCCTCACCT
    CCACCTGCTT GTTTCTCTGA GAAGCTGGAG AATGAGGTTA GCAATGCAAT TCTGAAACAG
    AGTGCAGAAG AAAAGGAATT TGTGGAAAAG CATAGCAGTA ATCTTCACAT GAGAGCAATG
    CCTCAAGATC AGCCTGTAAA CCAAAAACCC CAATCAGCTT TCTACTACTC CCGGCTGTTG
    CTTAGTATCC TTGGCATGAA CTCCTGGGAG AAGAGGCGAA ACTTCCACTT GCTTAAGAAG
    AATGAGAAAC TGTTACGTGA GCTGAAAAAT TTGGACTCCA GGCAGTGCCG CGAAACCCAC
    AAAATTGCAG TTTTCTACGT TGCAGACGGC CAGGAGGACA AGCATTCAAT CCTATCTAAC
    ACCGGTGGCA GTCAGGACTA TGAGGATTTT GTGGCTGGTC TTGGATGGGA GGTCAATCTT
    TCCAATCACT GTGGCTTCCT GGGAGGACTT CAGAGGAACA AGAGCACTGG CTTTAGCACT
    CCATATTTTG CAACCTCAAT AGTGGAGGTC ATGTTCCATG TCTCCACAAG AATGATTTCA
    GACTCTGATG ATTGTTTAAC CAAAAAGCTT CGCCACCTTG GGAATGATGA GGTGCACATT
    GTGTGGTCAG AACACAGTCG GGAATATCGC CGCGGCATCA TACCCACTGA ATTTGGGGAC
    GTCCTACTTG TAATATACCC CATGAAGAAC CACATGTTCA GCATCCAGAT TATGAAGAAG
    CCAGAAATCC CATTTTTTGG GCCCCTCTTC GATGGAGCGA TTGTGAATGG CAAAATCCTC
    CCAGGACTGG TTCGTGCAAC AGCCATCAAT GCCAGCAGAG CACTTAAATC TCTTATCCCC
    CTGTACCAAA ATTTCTTTGA GGAACGGATG CGATACTTGG ATTCAATCAC ACAGCAGCAT
    TTGGAACCCA CCACCTTTGA AGACTACGCT GCCCAGGTGT TCTGCCCAGT GCCGCACAAT
    CCCCTCCAGC TGAATGCACG GCCTTGCCCA ACGAAAGAGC AAGATGAGAA CGTATCGCCT
    CCGGGGGATG GATCAGAACT GGCTTTTTCT TTGTCACCAC GGAGCAGCAA AAGTCGCATG
    TCCATAAAGC TCCGGCGTTC CTCTGGCTCT GCCAACAAAA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746414.1
    CDS259..7743
    Translation

    Target ORF information:

    RefSeq Version XM_031890554.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGGTTTTCCA TTTGGAGGAG ATTCAACAGT CCCCATCTGT
    ATTGAAGCAG ATGCTGGCTC TACACATCTT AGCGTTGAGG AACCAGAAGT TGGAAGTCTT
    ACAGCACTGC ATATTGACTC TGAGACAAGC AGCTTCAATC AGCAGATTAT GTGCACGGAG
    GCAGTGGCTG TAACAGGGTC AGAAAGTGTT TCCCCTGCTT TGTCTGCTCA TGGGTCGAGA
    TCAGAGACTC CCTCTCCTGC AGCTCTAAAC TCAGATCAGG CAGAGCTACA CGACAAGCTT
    CAGCATTCGG TTTTACAAAC TCCCGATGAT ATAGAGCCTT GTGACTTTCC TTTAGAAAGC
    TGCAGTGTTA TGGCTGGAGG AACACTTACT GGATGGCACG CGGACGTTTC CACAGTCATG
    TGGAGAAGAA TGCTTGGGAT TCTTGGGGAT GTCAATAACA TTGCCAATCC TGAAATTCAT
    GCCCAAGTTT TTGATTACTT GTGTGAACTC TGGCAGAACC TGGCTAAGAT TAGAGATAAT
    TTGGGAATAT CCTTGGATAA TCAGTCTTCT CCCCGACCTC CTGTTCTTAT CCCACCCCTG
    AGGATTCTCA CTCCCTGGCT TTTTAAGGCT ACGATGCTGC CTGAAAAATA CAAGCAGGGC
    AAACTCCATG CTTACAAACT GATTTGCAAG ATAATGAAGA GAAGACAGGA CGTATCTCCA
    AACAGGGACT TCTTAACGCA CTTCTATAAC ATTATGCACT GTGGTCTTCT TCACAACGAT
    CAGGACATTG TGAATACTAT AATCAAGCAC TGTTCACCTC AATTCTTTGC CCTTGGCCTT
    CCTGGTGCCA CACTCCTTAT TATGGATTTT ATTGTAGCTG CTGGGAGAGT TGCTTCCTCG
    TCATCCCTTA ATGCTCCACG TGTGGAAGCT CAAGTTCTTC TGGGATCGCT TGTCTGTTTC
    CCCAACCTCT ACCAGGAATT GCCTGCTTTG CACCCAAATA GCTCTGACAT AGTAATGTCA
    CATTTTACAG ATGTCAAGGA ACTCACAATT AAAACTGTAC TAAATTCAGC CAGAGATGAA
    CCGTCTGGCC CTGCAAGATG TGTAGCATTG TGCAGTTTGG GAATATGGAT GTGTGAGGAG
    CTTGTCAATC AAACACATCA TCCACAAATC AAAGAAGCAC TCAATGTCAT CTGTGTCTCC
    TTAAAATTTC CCAATAAAGT GGTGGCCCAG GTCGCCTGCA GTGTGCTAAA TGTGCTGCTC
    AATTATGTTC AGCAGCTTCA GATTTACCAT TCAGACTACC CACTTAAAAT CATTGAGATC
    CTGCTAGCGA CAGTGACATA TCTGCTGCCA AGTGCTGAGA CCTCATCTTA CGAGCAAGAC
    AAGCGGTTGG TGGTCTCTCT GCTTCTTTCC CTTTTGGACT GGATGATGGC ATTACCCCTT
    GCATTGTTAT TGAAACCACT ACGCCATGCT CGGACGGAGA GTGATGGAAG GGAGAACTCT
    GTGTTAAACT GTGCATATAA GGTTCTACAT GACTGTGTCT ATGGCTCCCA GCATTTCAGC
    AATCCCAGGT ACTTTCCAAT GAGCCTTTCA GATCTGGCAT CGGAGGATTA TGATCCCTTT
    ATGCAACTGG AATGCCTGAA GGAACCAGAA CCTTTACATT CCCCAGATTC TGAGCGCTCC
    ACCAAACTGC AGCCTGTTAC TGAGGTGAAA ATTCCAAAGC AGCAAGGTCT TATTCCAGTA
    GCAGCACGGA CAGTAATCAC ACATTTGGTA AACCACTTGG GCCACTATCC AATGAGTGGA
    GACCCCGCAA TGTTGACTAG CCAGATCTGC GAGAACCATG ATAATCCATA CTGTGAAAGT
    TCGGATCTAT CTCCTCAACT CTTCCACAGC CCCAATCTGC AGTTCTTTGC TCTCAATAAT
    ACCACTCTTG TGTCCTGCCT TCAGATACAG TCTGAAGAGA AACTACCTCC TGGTGAGGAC
    CTCTCTTCTG CCAGATCAAC TGTACGCATA ATTATGCGGG ACCTTTCTGG CAAGTACAGT
    TGGGATTCCT CTATATTATA CGGACCTCCT CTCCCATGTG AGGGACAAAT GACTCAGTGG
    TCTTCCCTTC TGGCAACATT TTCTCCTCCT TCCAATCAAG AGGTTGAACA CCCATCAACC
    CAGAGGGAAT CTGAGGACTT TTCTTCTCTT TCTTCAAGAA GGCGAGGAAG GGAATCTCTG
    CCCCTATGGG ACAGCTTATC AGATGAACAA GATGCTCTTG ACTGCCTTTT GCAGTATCTG
    GGAGCTACTG GTCCAGAGTG CCTTCAGAGG GCTGGTGTTC CCCTCAATGT GCCCTCACCT
    CCACCTGCTT GTTTCTCTGA GAAGCTGGAG AATGAGGTTA GCAATGCAAT TCTGAAACAG
    AGTGCAGAAG AAAAGGAATT TGTGGAAAAG CATAGCAGTA ATCTTCACAT GAGAGCAATG
    CCTCAAGATC AGCCTGTAAA CCAAAAACCC CAATCAGCTT TCTACTACTC CCGGCTGTTG
    CTTAGTATCC TTGGCATGAA CTCCTGGGAG AAGAGGCGAA ACTTCCACTT GCTTAAGAAG
    AATGAGAAAC TGTTACGTGA GCTGAAAAAT TTGGACTCCA GGCAGTGCCG CGAAACCCAC
    AAAATTGCAG TTTTCTACGT TGCAGACGGC CAGGAGGACA AGCATTCAAT CCTATCTAAC
    ACCGGTGGCA GTCAGGACTA TGAGGATTTT GTGGCTGGTC TTGGATGGGA GGTCAATCTT
    TCCAATCACT GTGGCTTCCT GGGAGGACTT CAGAGGAACA AGAGCACTGG CTTTAGCACT
    CCATATTTTG CAACCTCAAT AGTGGAGGTC ATGTTCCATG TCTCCACAAG AATGATTTCA
    GACTCTGATG ATTGTTTAAC CAAAAAGCTT CGCCACCTTG GGAATGATGA GGTGCACATT
    GTGTGGTCAG AACACAGTCG GGAATATCGC CGCGGCATCA TACCCACTGA ATTTGGGGAC
    GTCCTACTTG TAATATACCC CATGAAGAAC CACATGTTCA GCATCCAGAT TATGAAGAAG
    CCAGAAATCC CATTTTTTGG GCCCCTCTTC GATGGAGCGA TTGTGAATGG CAAAATCCTC
    CCAGGACTGG TTCGTGCAAC AGCCATCAAT GCCAGCAGAG CACTTAAATC TCTTATCCCC
    CTGTACCAAA ATTTCTTTGA GGAACGGATG CGATACTTGG ATTCAATCAC ACAGCAGCAT
    TTGGAACCCA CCACCTTTGA AGACTACGCT GCCCAGGTGT TCTGCCCAGT GCCGCACAAT
    CCCCTCCAGC TGAATGCACG GCCTTGCCCA ACGAAAGAGC AAGATGAGAA CGTATCGCCT
    CCGGGGGATG GATCAGAACT GGCTTTTTCT TTGTCACCAC GGAGCAGCAA AAGTCGCATG
    TCCATAAAGC TCCGGCGTTC CTCTGGCTCT GCCAACAAAA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63777
    Clone ID Related Accession (Same CDS sequence) XM_031890555.1
    Accession Version XM_031890555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGCAGGTTTT CCATTTGGAG GAGATTCAAC AGTCCCCATC
    TGTATTGAAG CAGATGCTGG CTCTACACAT CTTAGCGTTG AGGAACCAGA AGTTGGAAGT
    CTTACAGCAC TGCATATTGA CTCTGAGACA AGCAGCTTCA ATCAGCAGAT TATGTGCACG
    GAGGCAGTGG CTGTAACAGG GTCAGAAAGT GTTTCCCCTG CTTTGTCTGC TCATGGGTCG
    AGATCAGAGA CTCCCTCTCC TGCAGCTCTA AACTCAGATC AGGCAGAGCT ACACGACAAG
    CTTCAGCATT CGGTTTTACA AACTCCCGAT GATATAGAAA GCTGCAGTGT TATGGCTGGA
    GGAACACTTA CTGGATGGCA CGCGGACGTT TCCACAGTCA TGTGGAGAAG AATGCTTGGG
    ATTCTTGGGG ATGTCAATAA CATTGCCAAT CCTGAAATTC ATGCCCAAGT TTTTGATTAC
    TTGTGTGAAC TCTGGCAGAA CCTGGCTAAG ATTAGAGATA ATTTGGGAAT ATCCTTGGAT
    AATCAGTCTT CTCCCCGACC TCCTGTTCTT ATCCCACCCC TGAGGATTCT CACTCCCTGG
    CTTTTTAAGG CTACGATGCT GCCTGAAAAA TACAAGCAGG GCAAACTCCA TGCTTACAAA
    CTGATTTGCA AGATAATGAA GAGAAGACAG GACGTATCTC CAAACAGGGA CTTCTTAACG
    CACTTCTATA ACATTATGCA CTGTGGTCTT CTTCACAACG ATCAGGACAT TGTGAATACT
    ATAATCAAGC ACTGTTCACC TCAATTCTTT GCCCTTGGCC TTCCTGGTGC CACACTCCTT
    ATTATGGATT TTATTGTAGC TGCTGGGAGA GTTGCTTCCT CGTCATCCCT TAATGCTCCA
    CGTGTGGAAG CTCAAGTTCT TCTGGGATCG CTTGTCTGTT TCCCCAACCT CTACCAGGAA
    TTGCCTGCTT TGCACCCAAA TAGCTCTGAC ATAGTAATGT CACATTTTAC AGATGTCAAG
    GAACTCACAA TTAAAACTGT ACTAAATTCA GCCAGAGATG AACCGTCTGG CCCTGCAAGA
    TGTGTAGCAT TGTGCAGTTT GGGAATATGG ATGTGTGAGG AGCTTGTCAA TCAAACACAT
    CATCCACAAA TCAAAGAAGC ACTCAATGTC ATCTGTGTCT CCTTAAAATT TCCCAATAAA
    GTGGTGGCCC AGGTCGCCTG CAGTGTGCTA AATGTGCTGC TCAATTATGT TCAGCAGCTT
    CAGATTTACC ATTCAGACTA CCCACTTAAA ATCATTGAGA TCCTGCTAGC GACAGTGACA
    TATCTGCTGC CAAGTGCTGA GACCTCATCT TACGAGCAAG ACAAGCGGTT GGTGGTCTCT
    CTGCTTCTTT CCCTTTTGGA CTGGATGATG GCATTACCCC TTGCATTGTT ATTGAAACCA
    CTACGCCATG CTCGGACGGA GAGTGATGGA AGGGAGAACT CTGTGTTAAA CTGTGCATAT
    AAGGTTCTAC ATGACTGTGT CTATGGCTCC CAGCATTTCA GCAATCCCAG GTACTTTCCA
    ATGAGCCTTT CAGATCTGGC ATCGGAGGAT TATGATCCCT TTATGCAACT GGAATGCCTG
    AAGGAACCAG AACCTTTACA TTCCCCAGAT TCTGAGCGCT CCACCAAACT GCAGCCTGTT
    ACTGAGGTGA AAATTCCAAA GCAGCAAGGT CTTATTCCAG TAGCAGCACG GACAGTAATC
    ACACATTTGG TAAACCACTT GGGCCACTAT CCAATGAGTG GAGACCCCGC AATGTTGACT
    AGCCAGATCT GCGAGAACCA TGATAATCCA TACTGTGAAA GTTCGGATCT ATCTCCTCAA
    CTCTTCCACA GCCCCAATCT GCAGTTCTTT GCTCTCAATA ATACCACTCT TGTGTCCTGC
    CTTCAGATAC AGTCTGAAGA GAAACTACCT CCTGGTGAGG ACCTCTCTTC TGCCAGATCA
    ACTGTACGCA TAATTATGCG GGACCTTTCT GGCAAGTACA GTTGGGATTC CTCTATATTA
    TACGGACCTC CTCTCCCATG TGAGGGACAA ATGACTCAGT GGTCTTCCCT TCTGGCAACA
    TTTTCTCCTC CTTCCAATCA AGAGGTTGAA CACCCATCAA CCCAGAGGGA ATCTGAGGAC
    TTTTCTTCTC TTTCTTCAAG AAGGCGAGGA AGGGAATCTC TGCCCCTATG GGACAGCTTA
    TCAGATGAAC AAGATGCTCT TGACTGCCTT TTGCAGTATC TGGGAGCTAC TGGTCCAGAG
    TGCCTTCAGA GGGCTGGTGT TCCCCTCAAT GTGCCCTCAC CTCCACCTGC TTGTTTCTCT
    GAGAAGCTGG AGAATGAGGT TAGCAATGCA ATTCTGAAAC AGAGTGCAGA AGAAAAGGAA
    TTTGTGGAAA AGCATAGCAG TAATCTTCAC ATGAGAGCAA TGCCTCAAGA TCAGCCTGTA
    AACCAAAAAC CCCAATCAGC TTTCTACTAC TCCCGGCTGT TGCTTAGTAT CCTTGGCATG
    AACTCCTGGG AGAAGAGGCG AAACTTCCAC TTGCTTAAGA AGAATGAGAA ACTGTTACGT
    GAGCTGAAAA ATTTGGACTC CAGGCAGTGC CGCGAAACCC ACAAAATTGC AGTTTTCTAC
    GTTGCAGACG GCCAGGAGGA CAAGCATTCA ATCCTATCTA ACACCGGTGG CAGTCAGGAC
    TATGAGGATT TTGTGGCTGG TCTTGGATGG GAGGTCAATC TTTCCAATCA CTGTGGCTTC
    CTGGGAGGAC TTCAGAGGAA CAAGAGCACT GGCTTTAGCA CTCCATATTT TGCAACCTCA
    ATAGTGGAGG TCATGTTCCA TGTCTCCACA AGAATGATTT CAGACTCTGA TGATTGTTTA
    ACCAAAAAGC TTCGCCACCT TGGGAATGAT GAGGTGCACA TTGTGTGGTC AGAACACAGT
    CGGGAATATC GCCGCGGCAT CATACCCACT GAATTTGGGG ACGTCCTACT TGTAATATAC
    CCCATGAAGA ACCACATGTT CAGCATCCAG ATTATGAAGA AGCCAGAAAT CCCATTTTTT
    GGGCCCCTCT TCGATGGAGC GATTGTGAAT GGCAAAATCC TCCCAGGACT GGTTCGTGCA
    ACAGCCATCA ATGCCAGCAG AGCACTTAAA TCTCTTATCC CCCTGTACCA AAATTTCTTT
    GAGGAACGGA TGCGATACTT GGATTCAATC ACACAGCAGC ATTTGGAACC CACCACCTTT
    GAAGACTACG CTGCCCAGGT GTTCTGCCCA GTGCCGCACA ATCCCCTCCA GCTGAATGCA
    CGGCCTTGCC CAACGAAAGA GCAAGATGAG AACGTATCGC CTCCGGGGGA TGGATCAGAA
    CTGGCTTTTT CTTTGTCACC ACGGAGCAGC AAAAGTCGCA TGTCCATAAA GCTCCGGCGT
    TCCTCTGGCT CTGCCAACAA AACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746415.1
    CDS260..7726
    Translation

    Target ORF information:

    RefSeq Version XM_031890555.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGCAGGTTTT CCATTTGGAG GAGATTCAAC AGTCCCCATC
    TGTATTGAAG CAGATGCTGG CTCTACACAT CTTAGCGTTG AGGAACCAGA AGTTGGAAGT
    CTTACAGCAC TGCATATTGA CTCTGAGACA AGCAGCTTCA ATCAGCAGAT TATGTGCACG
    GAGGCAGTGG CTGTAACAGG GTCAGAAAGT GTTTCCCCTG CTTTGTCTGC TCATGGGTCG
    AGATCAGAGA CTCCCTCTCC TGCAGCTCTA AACTCAGATC AGGCAGAGCT ACACGACAAG
    CTTCAGCATT CGGTTTTACA AACTCCCGAT GATATAGAAA GCTGCAGTGT TATGGCTGGA
    GGAACACTTA CTGGATGGCA CGCGGACGTT TCCACAGTCA TGTGGAGAAG AATGCTTGGG
    ATTCTTGGGG ATGTCAATAA CATTGCCAAT CCTGAAATTC ATGCCCAAGT TTTTGATTAC
    TTGTGTGAAC TCTGGCAGAA CCTGGCTAAG ATTAGAGATA ATTTGGGAAT ATCCTTGGAT
    AATCAGTCTT CTCCCCGACC TCCTGTTCTT ATCCCACCCC TGAGGATTCT CACTCCCTGG
    CTTTTTAAGG CTACGATGCT GCCTGAAAAA TACAAGCAGG GCAAACTCCA TGCTTACAAA
    CTGATTTGCA AGATAATGAA GAGAAGACAG GACGTATCTC CAAACAGGGA CTTCTTAACG
    CACTTCTATA ACATTATGCA CTGTGGTCTT CTTCACAACG ATCAGGACAT TGTGAATACT
    ATAATCAAGC ACTGTTCACC TCAATTCTTT GCCCTTGGCC TTCCTGGTGC CACACTCCTT
    ATTATGGATT TTATTGTAGC TGCTGGGAGA GTTGCTTCCT CGTCATCCCT TAATGCTCCA
    CGTGTGGAAG CTCAAGTTCT TCTGGGATCG CTTGTCTGTT TCCCCAACCT CTACCAGGAA
    TTGCCTGCTT TGCACCCAAA TAGCTCTGAC ATAGTAATGT CACATTTTAC AGATGTCAAG
    GAACTCACAA TTAAAACTGT ACTAAATTCA GCCAGAGATG AACCGTCTGG CCCTGCAAGA
    TGTGTAGCAT TGTGCAGTTT GGGAATATGG ATGTGTGAGG AGCTTGTCAA TCAAACACAT
    CATCCACAAA TCAAAGAAGC ACTCAATGTC ATCTGTGTCT CCTTAAAATT TCCCAATAAA
    GTGGTGGCCC AGGTCGCCTG CAGTGTGCTA AATGTGCTGC TCAATTATGT TCAGCAGCTT
    CAGATTTACC ATTCAGACTA CCCACTTAAA ATCATTGAGA TCCTGCTAGC GACAGTGACA
    TATCTGCTGC CAAGTGCTGA GACCTCATCT TACGAGCAAG ACAAGCGGTT GGTGGTCTCT
    CTGCTTCTTT CCCTTTTGGA CTGGATGATG GCATTACCCC TTGCATTGTT ATTGAAACCA
    CTACGCCATG CTCGGACGGA GAGTGATGGA AGGGAGAACT CTGTGTTAAA CTGTGCATAT
    AAGGTTCTAC ATGACTGTGT CTATGGCTCC CAGCATTTCA GCAATCCCAG GTACTTTCCA
    ATGAGCCTTT CAGATCTGGC ATCGGAGGAT TATGATCCCT TTATGCAACT GGAATGCCTG
    AAGGAACCAG AACCTTTACA TTCCCCAGAT TCTGAGCGCT CCACCAAACT GCAGCCTGTT
    ACTGAGGTGA AAATTCCAAA GCAGCAAGGT CTTATTCCAG TAGCAGCACG GACAGTAATC
    ACACATTTGG TAAACCACTT GGGCCACTAT CCAATGAGTG GAGACCCCGC AATGTTGACT
    AGCCAGATCT GCGAGAACCA TGATAATCCA TACTGTGAAA GTTCGGATCT ATCTCCTCAA
    CTCTTCCACA GCCCCAATCT GCAGTTCTTT GCTCTCAATA ATACCACTCT TGTGTCCTGC
    CTTCAGATAC AGTCTGAAGA GAAACTACCT CCTGGTGAGG ACCTCTCTTC TGCCAGATCA
    ACTGTACGCA TAATTATGCG GGACCTTTCT GGCAAGTACA GTTGGGATTC CTCTATATTA
    TACGGACCTC CTCTCCCATG TGAGGGACAA ATGACTCAGT GGTCTTCCCT TCTGGCAACA
    TTTTCTCCTC CTTCCAATCA AGAGGTTGAA CACCCATCAA CCCAGAGGGA ATCTGAGGAC
    TTTTCTTCTC TTTCTTCAAG AAGGCGAGGA AGGGAATCTC TGCCCCTATG GGACAGCTTA
    TCAGATGAAC AAGATGCTCT TGACTGCCTT TTGCAGTATC TGGGAGCTAC TGGTCCAGAG
    TGCCTTCAGA GGGCTGGTGT TCCCCTCAAT GTGCCCTCAC CTCCACCTGC TTGTTTCTCT
    GAGAAGCTGG AGAATGAGGT TAGCAATGCA ATTCTGAAAC AGAGTGCAGA AGAAAAGGAA
    TTTGTGGAAA AGCATAGCAG TAATCTTCAC ATGAGAGCAA TGCCTCAAGA TCAGCCTGTA
    AACCAAAAAC CCCAATCAGC TTTCTACTAC TCCCGGCTGT TGCTTAGTAT CCTTGGCATG
    AACTCCTGGG AGAAGAGGCG AAACTTCCAC TTGCTTAAGA AGAATGAGAA ACTGTTACGT
    GAGCTGAAAA ATTTGGACTC CAGGCAGTGC CGCGAAACCC ACAAAATTGC AGTTTTCTAC
    GTTGCAGACG GCCAGGAGGA CAAGCATTCA ATCCTATCTA ACACCGGTGG CAGTCAGGAC
    TATGAGGATT TTGTGGCTGG TCTTGGATGG GAGGTCAATC TTTCCAATCA CTGTGGCTTC
    CTGGGAGGAC TTCAGAGGAA CAAGAGCACT GGCTTTAGCA CTCCATATTT TGCAACCTCA
    ATAGTGGAGG TCATGTTCCA TGTCTCCACA AGAATGATTT CAGACTCTGA TGATTGTTTA
    ACCAAAAAGC TTCGCCACCT TGGGAATGAT GAGGTGCACA TTGTGTGGTC AGAACACAGT
    CGGGAATATC GCCGCGGCAT CATACCCACT GAATTTGGGG ACGTCCTACT TGTAATATAC
    CCCATGAAGA ACCACATGTT CAGCATCCAG ATTATGAAGA AGCCAGAAAT CCCATTTTTT
    GGGCCCCTCT TCGATGGAGC GATTGTGAAT GGCAAAATCC TCCCAGGACT GGTTCGTGCA
    ACAGCCATCA ATGCCAGCAG AGCACTTAAA TCTCTTATCC CCCTGTACCA AAATTTCTTT
    GAGGAACGGA TGCGATACTT GGATTCAATC ACACAGCAGC ATTTGGAACC CACCACCTTT
    GAAGACTACG CTGCCCAGGT GTTCTGCCCA GTGCCGCACA ATCCCCTCCA GCTGAATGCA
    CGGCCTTGCC CAACGAAAGA GCAAGATGAG AACGTATCGC CTCCGGGGGA TGGATCAGAA
    CTGGCTTTTT CTTTGTCACC ACGGAGCAGC AAAAGTCGCA TGTCCATAAA GCTCCGGCGT
    TCCTCTGGCT CTGCCAACAA AACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63778
    Clone ID Related Accession (Same CDS sequence) XM_031890556.1
    Accession Version XM_031890556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2521
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    2641
    2701
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    2941
    3001
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    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGCAGGTTTT CCATTTGGAG GAGATTCAAC AGTCCCCATC
    TGTATTGAAG CAGATGCTGG CTCTACACAT CTTAGCGTTG AGGAACCAGA AGTTGGAAGT
    CTTACAGCAC TGCATATTGA CTCTGAGACA AGCAGCTTCA ATCAGCAGAT TATGTGCACG
    GAGGCAGTGG CTGTAACAGG GTCAGAAAGT GTTTCCCCTG CTTTGTCTGC TCATGGGTCG
    AGATCAGAGA CTCCCTCTCC TGCAGCTCTA AACTCAGATC AGGCAGAGCT ACACGACAAG
    CTTCAGCATT CGGTTTTACA AACTCCCGAT GATATAGAGC CTTGTGACTT TCCTTTAGAA
    AGCTGCAGTG TTATGGCTGG AGGAACACTT ACTGGATGGC ACGCGGACGT TTCCACAGTC
    ATGTGGAGAA GAATGCTTGG GATTCTTGGG GATGTCAATA ACATTGCCAA TCCTGAAATT
    CATGCCCAAG TTTTTGATTA CTTGTGTGAA CTCTGGCAGA ACCTGGCTAA GATTAGAGAT
    AATTTGGGAA TATCCTTGGA TAATCAGTCT TCTCCCCGAC CTCCTGTTCT TATCCCACCC
    CTGAGGATTC TCACTCCCTG GCTTTTTAAG GCTACGATGC TGCCTGAAAA ATACAAGCAG
    GGCAAACTCC ATGCTTACAA ACTGATTTGC AAGATAATGA AGAGAAGACA GGACGTATCT
    CCAAACAGGG ACTTCTTAAC GCACTTCTAT AACATTATGC ACTGTGGTCT TCTTCACAAC
    GATCAGGACA TTGTGAATAC TATAATCAAG CACTGTTCAC CTCAATTCTT TGCCCTTGGC
    CTTCCTGGTG CCACACTCCT TATTATGGAT TTTATTGTAG CTGCTGGGAG AGTTGCTTCC
    TCGTCATCCC TTAATGCTCC ACGTGTGGAA GCTCAAGTTC TTCTGGGATC GCTTGTCTGT
    TTCCCCAACC TCTACCAGGA ATTGCCTGCT TTGCACCCAA ATAGCTCTGA CATAGTAATG
    TCACATTTTA CAGATGTCAA GGAACTCACA ATTAAAACTG TACTAAATTC AGCCAGAGAT
    GAACCGTCTG GCCCTGCAAG ATGTGTAGCA TTGTGCAGTT TGGGAATATG GATGTGTGAG
    GAGCTTGTCA ATCAAACACA TCATCCACAA ATCAAAGAAG CACTCAATGT CATCTGTGTC
    TCCTTAAAAT TTCCCAATAA AGTGGTGGCC CAGGTCGCCT GCAGTGTGCT AAATGTGCTG
    CTCAATTATG TTCAGCAGCT TCAGATTTAC CATTCAGACT ACCCACTTAA AATCATTGAG
    ATCCTGCTAG CGACAGTGAC ATATCTGCTG CCAAGTGCTG AGACCTCATC TTACGAGCAA
    GACAAGCGGT TGGTGGTCTC TCTGCTTCTT TCCCTTTTGG ACTGGATGAT GGCATTACCC
    CTTGCATTGT TATTGAAACC ACTACGCCAT GCTCGGACGG AGAGTGATGG AAGGGAGAAC
    TCTGTGTTAA ACTGTGCATA TAAGGTTCTA CATGACTGTG TCTATGGCTC CCAGCATTTC
    AGCAATCCCA GGTACTTTCC AATGAGCCTT TCAGATCTGG CATCGGAGGA TTATGATCCC
    TTTATGCAAC TGGAATGCCT GAAGGAACCA GAACCTTTAC ATTCCCCAGA TTCTGAGCGC
    TCCACCAAAC TGCAGCCTGT TACTGAGGTG AAAATTCCAA AGCAGCAAGG TCTTATTCCA
    GTAGCAGCAC GGACAGTAAT CACACATTTG GTAAACCACT TGGGCCACTA TCCAATGAGT
    GGAGACCCCG CAATGTTGAC TAGCCAGATC TGCGAGAACC ATGATAATCC ATACTGTGAA
    AGTTCGGATC TATCTCCTCA ACTCTTCCAC AGCCCCAATC TGCAGTTCTT TGCTCTCAAT
    AATACCACTC TTGTGTCCTG CCTTCAGATA CAGTCTGAAG AGAAACTACC TCCTGGTGAG
    GACCTCTCTT CTGCCAGATC AACTGTACGC ATAATTATGC GGGACCTTTC TGGCAAGTAC
    AGTTGGGATT CCTCTATATT ATACGGACCT CCTCTCCCAT GTGAGGGACA AATGACTCAG
    TGGTCTTCCC TTCTGGCAAC ATTTTCTCCT CCTTCCAATC AAGAGGTTGA ACACCCATCA
    ACCCAGAGGG AATCTGAGGA CTTTTCTTCT CTTTCTTCAA GAAGGCGAGG AAGGGAATCT
    CTGCCCCTAT GGGACAGCTT ATCAGATGAA CAAGATGCTC TTGACTGCCT TTTGCAGTAT
    CTGGGAGCTA CTGGTCCAGA GTGCCTTCAG AGGGCTGGTG TTCCCCTCAA TGTGCCCTCA
    CCTCCACCTG CTTGTTTCTC TGAGAAGCTG GAGAATGAGG TTAGCAATGC AATTCTGAAA
    CAGAGTGCAG AAGAAAAGGA ATTTGTGGAA AAGCATAGCA GTAATCTTCA CATGAGAGCA
    ATGCCTCAAG ATCAGCCTGT AAACCAAAAA CCCCAATCAG CTTTCTACTA CTCCCGGCTG
    TTGCTTAGTA TCCTTGGCAT GAACTCCTGG GAGAAGAGGC GAAACTTCCA CTTGCTTAAG
    AAGAATGAGA AACTGTTACG TGAGCTGAAA AATTTGGACT CCAGGCAGTG CCGCGAAACC
    CACAAAATTG CAGTTTTCTA CGTTGCAGAC GGCCAGGAGG ACAAGCATTC AATCCTATCT
    AACACCGGTG GCAGTCAGGA CTATGAGGAT TTTGTGGCTG GTCTTGGATG GGAGGTCAAT
    CTTTCCAATC ACTGTGGCTT CCTGGGAGGA CTTCAGAGGA ACAAGAGCAC TGGCTTTAGC
    ACTCCATATT TTGCAACCTC AATAGTGGAG GTCATGTTCC ATGTCTCCAC AAGAATGATT
    TCAGACTCTG ATGATTGTTT AACCAAAAAG CTTCGCCACC TTGGGAATGA TGAGGTGCAC
    ATTGTGTGGT CAGAACACAG TCGGGAATAT CGCCGCGGCA TCATACCCAC TGAATTTGGG
    GACGTCCTAC TTGTAATATA CCCCATGAAG AACCACATGT TCAGCATCCA GATTATGAAG
    AAGCCAGAAA TCCCATTTTT TGGGCCCCTC TTCGATGGAG CGATTGTGAA TGGCAAAATC
    CTCCCAGGAC TGGTTCGTGC AACAGCCATC AATGCCAGCA GAGCACTTAA ATCTCTTATC
    CCCCTGTACC AAAATTTCTT TGAGGAACGG ATGCGATACT TGGATTCAAT CACACAGCAG
    CATTTGGAAC CCACCACCTT TGAAGACTAC GCTGCCCAGG TGTTCTGCCC AGTGCCGCAC
    AATCCCCTCC AGCTGAATGC ACATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746416.1
    CDS260..7609
    Translation

    Target ORF information:

    RefSeq Version XM_031890556.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGGTGTGG TGTCTGGTAC AGATCATCTC
    ACCCCCCAGG CTTTGGACCC CCCCTCTTCT AACACACAGT CTCAGGAAGC ACCCATTACG
    CAGGTCGATG GCAGCATATA TGATCATCTC TGCCAGCTTG CTGGCTCAGC TGAATTAAGA
    GATGGAATAA AGGAACAGGG CTTGTACGAG GAATTAGAGG GTGAAGGTCT GTTGCCACGT
    TTGAGAGCCT ATTTAAGGGA GCAGGAAGTT CTAGAAGTAC TGAAAGACAG GACGGGCAAC
    TACCTGTTTC ATCAAGACAG CGAGCATTCT GAACATGTAG ATTGTTATAA TAAAGGGGAA
    GTCAGTTCAT TCATTCCTGA TTTATACAGT CAAGTAGTTT CTTCAGAGCA GAATGATAAC
    TGTTTAGGTG GGGTGGTTAA TATAGCCAAG AAAGCTGGTT GCATTAGGAT GAGCAACAGG
    TCACGAGAAG TCCATGTAAG GATACAGGTT GATGAAGAAG AAAGGGAGAA TGATTATGTT
    GCCCAGGAGC AAGGTGAGCG AAAGAGGGGC CTCTATAGGC AACAAGCAGC TGAGGGAGAG
    GAAACCTCTG ACTTGGCAAC TTCAAAGTTT CACCACTCCT CTGGCACTGA ACATGGGGCC
    AACATAAAGA ATCTTTGTGA GTCTGTAACA CACAAGGCTA AAGCAATGCG CCGGAAACGC
    AGCAGTGTTC AGGTTACCTT CCGTCCAGCC ACTGAGGCTG TGCTTTTCTA CAACTCTATG
    GAAAACAAAG ATGCCCCTTG GAAAGTGAGG CTGCGTAAAT TAAGTGGTTT AAGCACTAAC
    CCCTCTATAG CTGTAGAGCC AGGAAAATCC ACTGCCTATG CTGCACTTGG TGCAGTCAGA
    GTAGCATCAA GTGTTAGCCA GCCAGGTAAT GACCACCATG ATTTACCTTA CCCTCCCCCA
    CAAATCCAAA AGGAATGTGC CCGAATAAGG CAGAGATCCT GCAGTCAGGA GAGAGCCCCC
    TCTCATGCGT CATTGGGTGG CGTATATAAA ACTGTTGTCC ATGCTCTCTC AAAACCTAAG
    GCCAGTGTGT CCCTGCAGAA GCAACAAAAG ATGGCTACCG AGGCTCCACT GAAGGACCTG
    TACGGTCATG TTATGGGCTA TTTTGGAAGG AAGGCAGCAG CTACTAAAGA AGATTTAGCT
    CCCCAAGTCA ATGATGTTGC TGGTGCTGAA ATTGCCACTA ATGCCAATAT CCCAGACCTG
    ATGGATGAGT TTATAGCTGA GAGACTGCGA TCAGGCACCA GTGTGACTAT CACACGTAGG
    GGCAGCAGTT CTGGTAGTCT TGAGGTTCCC AAAGATTTAC CTGATATGCT TAATAAGCAT
    AGCCAGATGC GCCCAGCTGA TGACCCTGGT GTCCCTTCAG AGTGGACCTC TCCGGCAAGC
    GCAGCCAGCA GTGACCTTCT TAGCTCAGAC AGCCATTCGG ACTCTTTTAA TGCCTTCCAG
    TATGATGCAA GGAAGTTTGA AGCAGGTTTT CCATTTGGAG GAGATTCAAC AGTCCCCATC
    TGTATTGAAG CAGATGCTGG CTCTACACAT CTTAGCGTTG AGGAACCAGA AGTTGGAAGT
    CTTACAGCAC TGCATATTGA CTCTGAGACA AGCAGCTTCA ATCAGCAGAT TATGTGCACG
    GAGGCAGTGG CTGTAACAGG GTCAGAAAGT GTTTCCCCTG CTTTGTCTGC TCATGGGTCG
    AGATCAGAGA CTCCCTCTCC TGCAGCTCTA AACTCAGATC AGGCAGAGCT ACACGACAAG
    CTTCAGCATT CGGTTTTACA AACTCCCGAT GATATAGAGC CTTGTGACTT TCCTTTAGAA
    AGCTGCAGTG TTATGGCTGG AGGAACACTT ACTGGATGGC ACGCGGACGT TTCCACAGTC
    ATGTGGAGAA GAATGCTTGG GATTCTTGGG GATGTCAATA ACATTGCCAA TCCTGAAATT
    CATGCCCAAG TTTTTGATTA CTTGTGTGAA CTCTGGCAGA ACCTGGCTAA GATTAGAGAT
    AATTTGGGAA TATCCTTGGA TAATCAGTCT TCTCCCCGAC CTCCTGTTCT TATCCCACCC
    CTGAGGATTC TCACTCCCTG GCTTTTTAAG GCTACGATGC TGCCTGAAAA ATACAAGCAG
    GGCAAACTCC ATGCTTACAA ACTGATTTGC AAGATAATGA AGAGAAGACA GGACGTATCT
    CCAAACAGGG ACTTCTTAAC GCACTTCTAT AACATTATGC ACTGTGGTCT TCTTCACAAC
    GATCAGGACA TTGTGAATAC TATAATCAAG CACTGTTCAC CTCAATTCTT TGCCCTTGGC
    CTTCCTGGTG CCACACTCCT TATTATGGAT TTTATTGTAG CTGCTGGGAG AGTTGCTTCC
    TCGTCATCCC TTAATGCTCC ACGTGTGGAA GCTCAAGTTC TTCTGGGATC GCTTGTCTGT
    TTCCCCAACC TCTACCAGGA ATTGCCTGCT TTGCACCCAA ATAGCTCTGA CATAGTAATG
    TCACATTTTA CAGATGTCAA GGAACTCACA ATTAAAACTG TACTAAATTC AGCCAGAGAT
    GAACCGTCTG GCCCTGCAAG ATGTGTAGCA TTGTGCAGTT TGGGAATATG GATGTGTGAG
    GAGCTTGTCA ATCAAACACA TCATCCACAA ATCAAAGAAG CACTCAATGT CATCTGTGTC
    TCCTTAAAAT TTCCCAATAA AGTGGTGGCC CAGGTCGCCT GCAGTGTGCT AAATGTGCTG
    CTCAATTATG TTCAGCAGCT TCAGATTTAC CATTCAGACT ACCCACTTAA AATCATTGAG
    ATCCTGCTAG CGACAGTGAC ATATCTGCTG CCAAGTGCTG AGACCTCATC TTACGAGCAA
    GACAAGCGGT TGGTGGTCTC TCTGCTTCTT TCCCTTTTGG ACTGGATGAT GGCATTACCC
    CTTGCATTGT TATTGAAACC ACTACGCCAT GCTCGGACGG AGAGTGATGG AAGGGAGAAC
    TCTGTGTTAA ACTGTGCATA TAAGGTTCTA CATGACTGTG TCTATGGCTC CCAGCATTTC
    AGCAATCCCA GGTACTTTCC AATGAGCCTT TCAGATCTGG CATCGGAGGA TTATGATCCC
    TTTATGCAAC TGGAATGCCT GAAGGAACCA GAACCTTTAC ATTCCCCAGA TTCTGAGCGC
    TCCACCAAAC TGCAGCCTGT TACTGAGGTG AAAATTCCAA AGCAGCAAGG TCTTATTCCA
    GTAGCAGCAC GGACAGTAAT CACACATTTG GTAAACCACT TGGGCCACTA TCCAATGAGT
    GGAGACCCCG CAATGTTGAC TAGCCAGATC TGCGAGAACC ATGATAATCC ATACTGTGAA
    AGTTCGGATC TATCTCCTCA ACTCTTCCAC AGCCCCAATC TGCAGTTCTT TGCTCTCAAT
    AATACCACTC TTGTGTCCTG CCTTCAGATA CAGTCTGAAG AGAAACTACC TCCTGGTGAG
    GACCTCTCTT CTGCCAGATC AACTGTACGC ATAATTATGC GGGACCTTTC TGGCAAGTAC
    AGTTGGGATT CCTCTATATT ATACGGACCT CCTCTCCCAT GTGAGGGACA AATGACTCAG
    TGGTCTTCCC TTCTGGCAAC ATTTTCTCCT CCTTCCAATC AAGAGGTTGA ACACCCATCA
    ACCCAGAGGG AATCTGAGGA CTTTTCTTCT CTTTCTTCAA GAAGGCGAGG AAGGGAATCT
    CTGCCCCTAT GGGACAGCTT ATCAGATGAA CAAGATGCTC TTGACTGCCT TTTGCAGTAT
    CTGGGAGCTA CTGGTCCAGA GTGCCTTCAG AGGGCTGGTG TTCCCCTCAA TGTGCCCTCA
    CCTCCACCTG CTTGTTTCTC TGAGAAGCTG GAGAATGAGG TTAGCAATGC AATTCTGAAA
    CAGAGTGCAG AAGAAAAGGA ATTTGTGGAA AAGCATAGCA GTAATCTTCA CATGAGAGCA
    ATGCCTCAAG ATCAGCCTGT AAACCAAAAA CCCCAATCAG CTTTCTACTA CTCCCGGCTG
    TTGCTTAGTA TCCTTGGCAT GAACTCCTGG GAGAAGAGGC GAAACTTCCA CTTGCTTAAG
    AAGAATGAGA AACTGTTACG TGAGCTGAAA AATTTGGACT CCAGGCAGTG CCGCGAAACC
    CACAAAATTG CAGTTTTCTA CGTTGCAGAC GGCCAGGAGG ACAAGCATTC AATCCTATCT
    AACACCGGTG GCAGTCAGGA CTATGAGGAT TTTGTGGCTG GTCTTGGATG GGAGGTCAAT
    CTTTCCAATC ACTGTGGCTT CCTGGGAGGA CTTCAGAGGA ACAAGAGCAC TGGCTTTAGC
    ACTCCATATT TTGCAACCTC AATAGTGGAG GTCATGTTCC ATGTCTCCAC AAGAATGATT
    TCAGACTCTG ATGATTGTTT AACCAAAAAG CTTCGCCACC TTGGGAATGA TGAGGTGCAC
    ATTGTGTGGT CAGAACACAG TCGGGAATAT CGCCGCGGCA TCATACCCAC TGAATTTGGG
    GACGTCCTAC TTGTAATATA CCCCATGAAG AACCACATGT TCAGCATCCA GATTATGAAG
    AAGCCAGAAA TCCCATTTTT TGGGCCCCTC TTCGATGGAG CGATTGTGAA TGGCAAAATC
    CTCCCAGGAC TGGTTCGTGC AACAGCCATC AATGCCAGCA GAGCACTTAA ATCTCTTATC
    CCCCTGTACC AAAATTTCTT TGAGGAACGG ATGCGATACT TGGATTCAAT CACACAGCAG
    CATTTGGAAC CCACCACCTT TGAAGACTAC GCTGCCCAGG TGTTCTGCCC AGTGCCGCAC
    AATCCCCTCC AGCTGAATGC ACATCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63779
    Clone ID Related Accession (Same CDS sequence) XM_031890557.1
    Accession Version XM_031890557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGGTGTGGT GTCTGGTACA GATCATCTCA CCCCCCAGGC TTTGGACCCC
    CCCTCTTCTA ACACACAGTC TCAGGAAGCA CCCATTACGC AGGTCGATGG CAGCATATAT
    GATCATCTCT GCCAGCTTGC TGGCTCAGCT GAATTAAGAG ATGGAATAAA GGAACAGGGC
    TTGTACGAGG AATTAGAGGG TGAAGGTCTG TTGCCACGTT TGAGAGCCTA TTTAAGGGAG
    CAGGAAGTTC TAGAAGTACT GAAAGACAGG ACGGGCAACT ACCTGTTTCA TCAAGACAGC
    GAGCATTCTG AACATGTAGA TTGTTATAAT AAAGGGGAAG TCAGTTCATT CATTCCTGAT
    TTATACAGTC AAGTAGTTTC TTCAGAGCAG AATGATAACT GTTTAGGTGG GGTGGTTAAT
    ATAGCCAAGA AAGCTGGTTG CATTAGGATG AGCAACAGGT CACGAGAAGT CCATGTAAGG
    ATACAGGTTG ATGAAGAAGA AAGGGAGAAT GATTATGTTG CCCAGGAGCA AGGTGAGCGA
    AAGAGGGGCC TCTATAGGCA ACAAGCAGCT GAGGGAGAGG AAACCTCTGA CTTGGCAACT
    TCAAAGTTTC ACCACTCCTC TGGCACTGAA CATGGGGCCA ACATAAAGAA TCTTTGTGAG
    TCTGTAACAC ACAAGGCTAA AGCAATGCGC CGGAAACGCA GCAGTGTTCA GGTTACCTTC
    CGTCCAGCCA CTGAGGCTGT GCTTTTCTAC AACTCTATGG AAAACAAAGA TGCCCCTTGG
    AAAGTGAGGC TGCGTAAATT AAGTGGTTTA AGCACTAACC CCTCTATAGC TGTAGAGCCA
    GGAAAATCCA CTGCCTATGC TGCACTTGGT GCAGTCAGAG TAGCATCAAG TGTTAGCCAG
    CCAGGTAATG ACCACCATGA TTTACCTTAC CCTCCCCCAC AAATCCAAAA GGAATGTGCC
    CGAATAAGGC AGAGATCCTG CAGTCAGGAG AGAGCCCCCT CTCATGCGTC ATTGGGTGGC
    GTATATAAAA CTGTTGTCCA TGCTCTCTCA AAACCTAAGG CCAGTGTGTC CCTGCAGAAG
    CAACAAAAGA TGGCTACCGA GGCTCCACTG AAGGACCTGT ACGGTCATGT TATGGGCTAT
    TTTGGAAGGA AGGCAGCAGC TACTAAAGAA GATTTAGCTC CCCAAGTCAA TGATGTTGCT
    GGTGCTGAAA TTGCCACTAA TGCCAATATC CCAGACCTGA TGGATGAGTT TATAGCTGAG
    AGACTGCGAT CAGGCACCAG TGTGACTATC ACACGTAGGG GCAGCAGTTC TGGTAGTCTT
    GAGGTTCCCA AAGATTTACC TGATATGCTT AATAAGCATA GCCAGATGCG CCCAGCTGAT
    GACCCTGGTG TCCCTTCAGA GTGGACCTCT CCGGCAAGCG CAGCCAGCAG TGACCTTCTT
    AGCTCAGACA GCCATTCGGA CTCTTTTAAT GCCTTCCAGT ATGATGCAAG GAAGTTTGAA
    GCAGGTTTTC CATTTGGAGG AGATTCAACA GTCCCCATCT GTATTGAAGC AGATGCTGGC
    TCTACACATC TTAGCGTTGA GGAACCAGAA GTTGGAAGTC TTACAGCACT GCATATTGAC
    TCTGAGACAA GCAGCTTCAA TCAGCAGATT ATGTGCACGG AGGCAGTGGC TGTAACAGGG
    TCAGAAAGTG TTTCCCCTGC TTTGTCTGCT CATGGGTCGA GATCAGAGAC TCCCTCTCCT
    GCAGCTCTAA ACTCAGATCA GGCAGAGCTA CACGACAAGC TTCAGCATTC GGTTTTACAA
    ACTCCCGATG ATATAGAGCC TTGTGACTTT CCTTTAGAAA GCTGCAGTGT TATGGCTGGA
    GGAACACTTA CTGGATGGCA CGCGGACGTT TCCACAGTCA TGTGGAGAAG AATGCTTGGG
    ATTCTTGGGG ATGTCAATAA CATTGCCAAT CCTGAAATTC ATGCCCAAGT TTTTGATTAC
    TTGTGTGAAC TCTGGCAGAA CCTGGCTAAG ATTAGAGATA ATTTGGGAAT ATCCTTGGAT
    AATCAGTCTT CTCCCCGACC TCCTGTTCTT ATCCCACCCC TGAGGATTCT CACTCCCTGG
    CTTTTTAAGG CTACGATGCT GCCTGAAAAA TACAAGCAGG GCAAACTCCA TGCTTACAAA
    CTGATTTGCA AGATAATGAA GAGAAGACAG GACGTATCTC CAAACAGGGA CTTCTTAACG
    CACTTCTATA ACATTATGCA CTGTGGTCTT CTTCACAACG ATCAGGACAT TGTGAATACT
    ATAATCAAGC ACTGTTCACC TCAATTCTTT GCCCTTGGCC TTCCTGGTGC CACACTCCTT
    ATTATGGATT TTATTGTAGC TGCTGGGAGA GTTGCTTCCT CGTCATCCCT TAATGCTCCA
    CGTGTGGAAG CTCAAGTTCT TCTGGGATCG CTTGTCTGTT TCCCCAACCT CTACCAGGAA
    TTGCCTGCTT TGCACCCAAA TAGCTCTGAC ATAGTAATGT CACATTTTAC AGATGTCAAG
    GAACTCACAA TTAAAACTGT ACTAAATTCA GCCAGAGATG AACCGTCTGG CCCTGCAAGA
    TGTGTAGCAT TGTGCAGTTT GGGAATATGG ATGTGTGAGG AGCTTGTCAA TCAAACACAT
    CATCCACAAA TCAAAGAAGC ACTCAATGTC ATCTGTGTCT CCTTAAAATT TCCCAATAAA
    GTGGTGGCCC AGGTCGCCTG CAGTGTGCTA AATGTGCTGC TCAATTATGT TCAGCAGCTT
    CAGATTTACC ATTCAGACTA CCCACTTAAA ATCATTGAGA TCCTGCTAGC GACAGTGACA
    TATCTGCTGC CAAGTGCTGA GACCTCATCT TACGAGCAAG ACAAGCGGTT GGTGGTCTCT
    CTGCTTCTTT CCCTTTTGGA CTGGATGATG GCATTACCCC TTGCATTGTT ATTGAAACCA
    CTACGCCATG CTCGGACGGA GAGTGATGGA AGGGAGAACT CTGTGTTAAA CTGTGCATAT
    AAGGTTCTAC ATGACTGTGT CTATGGCTCC CAGCATTTCA GCAATCCCAG GTACTTTCCA
    ATGAGCCTTT CAGATCTGGC ATCGGAGGAT TATGATCCCT TTATGCAACT GGAATGCCTG
    AAGGAACCAG AACCTTTACA TTCCCCAGAT TCTGAGCGCT CCACCAAACT GCAGCCTGTT
    ACTGAGGTGA AAATTCCAAA GCAGCAAGGT CTTATTCCAG TAGCAGCACG GACAGTAATC
    ACACATTTGG TAAACCACTT GGGCCACTAT CCAATGAGTG GAGACCCCGC AATGTTGACT
    AGCCAGATCT GCGAGAACCA TGATAATCCA TACTGTGAAA GTTCGGATCT ATCTCCTCAA
    CTCTTCCACA GCCCCAATCT GCAGTTCTTT GCTCTCAATA ATACCACTCT TGTGTCCTGC
    CTTCAGATAC AGTCTGAAGA GAAACTACCT CCTGGTGAGG ACCTCTCTTC TGCCAGATCA
    ACTGTACGCA TAATTATGCG GGACCTTTCT GGCAAGTACA GTTGGGATTC CTCTATATTA
    TACGGACCTC CTCTCCCATG TGAGGGACAA ATGACTCAGT GGTCTTCCCT TCTGGCAACA
    TTTTCTCCTC CTTCCAATCA AGAGGTTGAA CACCCATCAA CCCAGAGGGA ATCTGAGGAC
    TTTTCTTCTC TTTCTTCAAG AAGGCGAGGA AGGGAATCTC TGCCCCTATG GGACAGCTTA
    TCAGATGAAC AAGATGCTCT TGACTGCCTT TTGCAGTATC TGGGAGCTAC TGGTCCAGAG
    TGCCTTCAGA GGGCTGGTGT TCCCCTCAAT GTGCCCTCAC CTCCACCTGC TTGTTTCTCT
    GAGAAGCTGG AGAATGAGGT TAGCAATGCA ATTCTGAAAC AGAGTGCAGA AGAAAAGGAA
    TTTGTGGAAA AGCATAGCAG TAATCTTCAC ATGAGAGCAA TGCCTCAAGA TCAGCCTGTA
    AACCAAAAAC CCCAATCAGC TTTCTACTAC TCCCGGCTGT TGCTTAGTAT CCTTGGCATG
    AACTCCTGGG AGAAGAGGCG AAACTTCCAC TTGCTTAAGA AGAATGAGAA ACTGTTACGT
    GAGCTGAAAA ATTTGGACTC CAGGCAGTGC CGCGAAACCC ACAAAATTGC AGTTTTCTAC
    GTTGCAGACG GCCAGGAGGA CAAGCATTCA ATCCTATCTA ACACCGGTGG CAGTCAGGAC
    TATGAGGATT TTGTGGCTGG TCTTGGATGG GAGGTCAATC TTTCCAATCA CTGTGGCTTC
    CTGGGAGGAC TTCAGAGGAA CAAGAGCACT GGCTTTAGCA CTCCATATTT TGCAACCTCA
    ATAGTGGAGG TCATGTTCCA TGTCTCCACA AGAATGATTT CAGACTCTGA TGATTGTTTA
    ACCAAAAAGC TTCGCCACCT TGGGAATGAT GAGGTGCACA TTGTGTGGTC AGAACACAGT
    CGGGAATATC GCCGCGGCAT CATACCCACT GAATTTGGGG ACGTCCTACT TGTAATATAC
    CCCATGAAGA ACCACATGTT CAGCATCCAG ATTATGAAGA AGCCAGAAAT CCCATTTTTT
    GGGCCCCTCT TCGATGGAGC GATTGTGAAT GGCAAAATCC TCCCAGGACT GGTTCGTGCA
    ACAGCCATCA ATGCCAGCAG AGCACTTAAA TCTCTTATCC CCCTGTACCA AAATTTCTTT
    GAGGAACGGA TGCGATACTT GGATTCAATC ACACAGCAGC ATTTGGAACC CACCACCTTT
    GAAGACTACG CTGCCCAGGT GTTCTGCCCA GTGCCGCACA ATCCCCTCCA GCTGAATGCA
    CGGCCTTGCC CAACGAAAGA GCAAGATGAG AACGTATCGC CTCCGGGGGA TGGATCAGAA
    CTGGCTTTTT CTTTGTCACC ACGGAGCAGC AAAAGTCGCA TGTCCATAAA GCTCCGGCGT
    TCCTCTGGCT CTGCCAACAA AACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746417.1
    CDS258..7604
    Translation

    Target ORF information:

    RefSeq Version XM_031890557.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGGTGTGGT GTCTGGTACA GATCATCTCA CCCCCCAGGC TTTGGACCCC
    CCCTCTTCTA ACACACAGTC TCAGGAAGCA CCCATTACGC AGGTCGATGG CAGCATATAT
    GATCATCTCT GCCAGCTTGC TGGCTCAGCT GAATTAAGAG ATGGAATAAA GGAACAGGGC
    TTGTACGAGG AATTAGAGGG TGAAGGTCTG TTGCCACGTT TGAGAGCCTA TTTAAGGGAG
    CAGGAAGTTC TAGAAGTACT GAAAGACAGG ACGGGCAACT ACCTGTTTCA TCAAGACAGC
    GAGCATTCTG AACATGTAGA TTGTTATAAT AAAGGGGAAG TCAGTTCATT CATTCCTGAT
    TTATACAGTC AAGTAGTTTC TTCAGAGCAG AATGATAACT GTTTAGGTGG GGTGGTTAAT
    ATAGCCAAGA AAGCTGGTTG CATTAGGATG AGCAACAGGT CACGAGAAGT CCATGTAAGG
    ATACAGGTTG ATGAAGAAGA AAGGGAGAAT GATTATGTTG CCCAGGAGCA AGGTGAGCGA
    AAGAGGGGCC TCTATAGGCA ACAAGCAGCT GAGGGAGAGG AAACCTCTGA CTTGGCAACT
    TCAAAGTTTC ACCACTCCTC TGGCACTGAA CATGGGGCCA ACATAAAGAA TCTTTGTGAG
    TCTGTAACAC ACAAGGCTAA AGCAATGCGC CGGAAACGCA GCAGTGTTCA GGTTACCTTC
    CGTCCAGCCA CTGAGGCTGT GCTTTTCTAC AACTCTATGG AAAACAAAGA TGCCCCTTGG
    AAAGTGAGGC TGCGTAAATT AAGTGGTTTA AGCACTAACC CCTCTATAGC TGTAGAGCCA
    GGAAAATCCA CTGCCTATGC TGCACTTGGT GCAGTCAGAG TAGCATCAAG TGTTAGCCAG
    CCAGGTAATG ACCACCATGA TTTACCTTAC CCTCCCCCAC AAATCCAAAA GGAATGTGCC
    CGAATAAGGC AGAGATCCTG CAGTCAGGAG AGAGCCCCCT CTCATGCGTC ATTGGGTGGC
    GTATATAAAA CTGTTGTCCA TGCTCTCTCA AAACCTAAGG CCAGTGTGTC CCTGCAGAAG
    CAACAAAAGA TGGCTACCGA GGCTCCACTG AAGGACCTGT ACGGTCATGT TATGGGCTAT
    TTTGGAAGGA AGGCAGCAGC TACTAAAGAA GATTTAGCTC CCCAAGTCAA TGATGTTGCT
    GGTGCTGAAA TTGCCACTAA TGCCAATATC CCAGACCTGA TGGATGAGTT TATAGCTGAG
    AGACTGCGAT CAGGCACCAG TGTGACTATC ACACGTAGGG GCAGCAGTTC TGGTAGTCTT
    GAGGTTCCCA AAGATTTACC TGATATGCTT AATAAGCATA GCCAGATGCG CCCAGCTGAT
    GACCCTGGTG TCCCTTCAGA GTGGACCTCT CCGGCAAGCG CAGCCAGCAG TGACCTTCTT
    AGCTCAGACA GCCATTCGGA CTCTTTTAAT GCCTTCCAGT ATGATGCAAG GAAGTTTGAA
    GCAGGTTTTC CATTTGGAGG AGATTCAACA GTCCCCATCT GTATTGAAGC AGATGCTGGC
    TCTACACATC TTAGCGTTGA GGAACCAGAA GTTGGAAGTC TTACAGCACT GCATATTGAC
    TCTGAGACAA GCAGCTTCAA TCAGCAGATT ATGTGCACGG AGGCAGTGGC TGTAACAGGG
    TCAGAAAGTG TTTCCCCTGC TTTGTCTGCT CATGGGTCGA GATCAGAGAC TCCCTCTCCT
    GCAGCTCTAA ACTCAGATCA GGCAGAGCTA CACGACAAGC TTCAGCATTC GGTTTTACAA
    ACTCCCGATG ATATAGAGCC TTGTGACTTT CCTTTAGAAA GCTGCAGTGT TATGGCTGGA
    GGAACACTTA CTGGATGGCA CGCGGACGTT TCCACAGTCA TGTGGAGAAG AATGCTTGGG
    ATTCTTGGGG ATGTCAATAA CATTGCCAAT CCTGAAATTC ATGCCCAAGT TTTTGATTAC
    TTGTGTGAAC TCTGGCAGAA CCTGGCTAAG ATTAGAGATA ATTTGGGAAT ATCCTTGGAT
    AATCAGTCTT CTCCCCGACC TCCTGTTCTT ATCCCACCCC TGAGGATTCT CACTCCCTGG
    CTTTTTAAGG CTACGATGCT GCCTGAAAAA TACAAGCAGG GCAAACTCCA TGCTTACAAA
    CTGATTTGCA AGATAATGAA GAGAAGACAG GACGTATCTC CAAACAGGGA CTTCTTAACG
    CACTTCTATA ACATTATGCA CTGTGGTCTT CTTCACAACG ATCAGGACAT TGTGAATACT
    ATAATCAAGC ACTGTTCACC TCAATTCTTT GCCCTTGGCC TTCCTGGTGC CACACTCCTT
    ATTATGGATT TTATTGTAGC TGCTGGGAGA GTTGCTTCCT CGTCATCCCT TAATGCTCCA
    CGTGTGGAAG CTCAAGTTCT TCTGGGATCG CTTGTCTGTT TCCCCAACCT CTACCAGGAA
    TTGCCTGCTT TGCACCCAAA TAGCTCTGAC ATAGTAATGT CACATTTTAC AGATGTCAAG
    GAACTCACAA TTAAAACTGT ACTAAATTCA GCCAGAGATG AACCGTCTGG CCCTGCAAGA
    TGTGTAGCAT TGTGCAGTTT GGGAATATGG ATGTGTGAGG AGCTTGTCAA TCAAACACAT
    CATCCACAAA TCAAAGAAGC ACTCAATGTC ATCTGTGTCT CCTTAAAATT TCCCAATAAA
    GTGGTGGCCC AGGTCGCCTG CAGTGTGCTA AATGTGCTGC TCAATTATGT TCAGCAGCTT
    CAGATTTACC ATTCAGACTA CCCACTTAAA ATCATTGAGA TCCTGCTAGC GACAGTGACA
    TATCTGCTGC CAAGTGCTGA GACCTCATCT TACGAGCAAG ACAAGCGGTT GGTGGTCTCT
    CTGCTTCTTT CCCTTTTGGA CTGGATGATG GCATTACCCC TTGCATTGTT ATTGAAACCA
    CTACGCCATG CTCGGACGGA GAGTGATGGA AGGGAGAACT CTGTGTTAAA CTGTGCATAT
    AAGGTTCTAC ATGACTGTGT CTATGGCTCC CAGCATTTCA GCAATCCCAG GTACTTTCCA
    ATGAGCCTTT CAGATCTGGC ATCGGAGGAT TATGATCCCT TTATGCAACT GGAATGCCTG
    AAGGAACCAG AACCTTTACA TTCCCCAGAT TCTGAGCGCT CCACCAAACT GCAGCCTGTT
    ACTGAGGTGA AAATTCCAAA GCAGCAAGGT CTTATTCCAG TAGCAGCACG GACAGTAATC
    ACACATTTGG TAAACCACTT GGGCCACTAT CCAATGAGTG GAGACCCCGC AATGTTGACT
    AGCCAGATCT GCGAGAACCA TGATAATCCA TACTGTGAAA GTTCGGATCT ATCTCCTCAA
    CTCTTCCACA GCCCCAATCT GCAGTTCTTT GCTCTCAATA ATACCACTCT TGTGTCCTGC
    CTTCAGATAC AGTCTGAAGA GAAACTACCT CCTGGTGAGG ACCTCTCTTC TGCCAGATCA
    ACTGTACGCA TAATTATGCG GGACCTTTCT GGCAAGTACA GTTGGGATTC CTCTATATTA
    TACGGACCTC CTCTCCCATG TGAGGGACAA ATGACTCAGT GGTCTTCCCT TCTGGCAACA
    TTTTCTCCTC CTTCCAATCA AGAGGTTGAA CACCCATCAA CCCAGAGGGA ATCTGAGGAC
    TTTTCTTCTC TTTCTTCAAG AAGGCGAGGA AGGGAATCTC TGCCCCTATG GGACAGCTTA
    TCAGATGAAC AAGATGCTCT TGACTGCCTT TTGCAGTATC TGGGAGCTAC TGGTCCAGAG
    TGCCTTCAGA GGGCTGGTGT TCCCCTCAAT GTGCCCTCAC CTCCACCTGC TTGTTTCTCT
    GAGAAGCTGG AGAATGAGGT TAGCAATGCA ATTCTGAAAC AGAGTGCAGA AGAAAAGGAA
    TTTGTGGAAA AGCATAGCAG TAATCTTCAC ATGAGAGCAA TGCCTCAAGA TCAGCCTGTA
    AACCAAAAAC CCCAATCAGC TTTCTACTAC TCCCGGCTGT TGCTTAGTAT CCTTGGCATG
    AACTCCTGGG AGAAGAGGCG AAACTTCCAC TTGCTTAAGA AGAATGAGAA ACTGTTACGT
    GAGCTGAAAA ATTTGGACTC CAGGCAGTGC CGCGAAACCC ACAAAATTGC AGTTTTCTAC
    GTTGCAGACG GCCAGGAGGA CAAGCATTCA ATCCTATCTA ACACCGGTGG CAGTCAGGAC
    TATGAGGATT TTGTGGCTGG TCTTGGATGG GAGGTCAATC TTTCCAATCA CTGTGGCTTC
    CTGGGAGGAC TTCAGAGGAA CAAGAGCACT GGCTTTAGCA CTCCATATTT TGCAACCTCA
    ATAGTGGAGG TCATGTTCCA TGTCTCCACA AGAATGATTT CAGACTCTGA TGATTGTTTA
    ACCAAAAAGC TTCGCCACCT TGGGAATGAT GAGGTGCACA TTGTGTGGTC AGAACACAGT
    CGGGAATATC GCCGCGGCAT CATACCCACT GAATTTGGGG ACGTCCTACT TGTAATATAC
    CCCATGAAGA ACCACATGTT CAGCATCCAG ATTATGAAGA AGCCAGAAAT CCCATTTTTT
    GGGCCCCTCT TCGATGGAGC GATTGTGAAT GGCAAAATCC TCCCAGGACT GGTTCGTGCA
    ACAGCCATCA ATGCCAGCAG AGCACTTAAA TCTCTTATCC CCCTGTACCA AAATTTCTTT
    GAGGAACGGA TGCGATACTT GGATTCAATC ACACAGCAGC ATTTGGAACC CACCACCTTT
    GAAGACTACG CTGCCCAGGT GTTCTGCCCA GTGCCGCACA ATCCCCTCCA GCTGAATGCA
    CGGCCTTGCC CAACGAAAGA GCAAGATGAG AACGTATCGC CTCCGGGGGA TGGATCAGAA
    CTGGCTTTTT CTTTGTCACC ACGGAGCAGC AAAAGTCGCA TGTCCATAAA GCTCCGGCGT
    TCCTCTGGCT CTGCCAACAA AACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63780
    Clone ID Related Accession (Same CDS sequence) XM_031890558.1
    Accession Version XM_031890558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGCTACTA AAGAAGATTT AGCTCCCCAA
    GTCAATGATG TTGCTGGTGC TGAAATTGCC ACTAATGCCA ATATCCCAGA CCTGATGGAT
    GAGTTTATAG CTGAGAGACT GCGATCAGGC ACCAGTGTGA CTATCACACG TAGGGGCAGC
    AGTTCTGGTA GTCTTGAGGT TCCCAAAGAT TTACCTGATA TGCTTAATAA GCATAGCCAG
    ATGCGCCCAG CTGATGACCC TGGTGTCCCT TCAGAGTGGA CCTCTCCGGC AAGCGCAGCC
    AGCAGTGACC TTCTTAGCTC AGACAGCCAT TCGGACTCTT TTAATGCCTT CCAGTATGAT
    GCAAGGAAGT TTGAAGCAGG TTTTCCATTT GGAGGAGATT CAACAGTCCC CATCTGTATT
    GAAGCAGATG CTGGCTCTAC ACATCTTAGC GTTGAGGAAC CAGAAGTTGG AAGTCTTACA
    GCACTGCATA TTGACTCTGA GACAAGCAGC TTCAATCAGC AGATTATGTG CACGGAGGCA
    GTGGCTGTAA CAGGGTCAGA AAGTGTTTCC CCTGCTTTGT CTGCTCATGG GTCGAGATCA
    GAGACTCCCT CTCCTGCAGC TCTAAACTCA GATCAGGCAG AGCTACACGA CAAGCTTCAG
    CATTCGGTTT TACAAACTCC CGATGATATA GAGCCTTGTG ACTTTCCTTT AGAAAGCTGC
    AGTGTTATGG CTGGAGGAAC ACTTACTGGA TGGCACGCGG ACGTTTCCAC AGTCATGTGG
    AGAAGAATGC TTGGGATTCT TGGGGATGTC AATAACATTG CCAATCCTGA AATTCATGCC
    CAAGTTTTTG ATTACTTGTG TGAACTCTGG CAGAACCTGG CTAAGATTAG AGATAATTTG
    GGAATATCCT TGGATAATCA GTCTTCTCCC CGACCTCCTG TTCTTATCCC ACCCCTGAGG
    ATTCTCACTC CCTGGCTTTT TAAGGCTACG ATGCTGCCTG AAAAATACAA GCAGGGCAAA
    CTCCATGCTT ACAAACTGAT TTGCAAGATA ATGAAGAGAA GACAGGACGT ATCTCCAAAC
    AGGGACTTCT TAACGCACTT CTATAACATT ATGCACTGTG GTCTTCTTCA CAACGATCAG
    GACATTGTGA ATACTATAAT CAAGCACTGT TCACCTCAAT TCTTTGCCCT TGGCCTTCCT
    GGTGCCACAC TCCTTATTAT GGATTTTATT GTAGCTGCTG GGAGAGTTGC TTCCTCGTCA
    TCCCTTAATG CTCCACGTGT GGAAGCTCAA GTTCTTCTGG GATCGCTTGT CTGTTTCCCC
    AACCTCTACC AGGAATTGCC TGCTTTGCAC CCAAATAGCT CTGACATAGT AATGTCACAT
    TTTACAGATG TCAAGGAACT CACAATTAAA ACTGTACTAA ATTCAGCCAG AGATGAACCG
    TCTGGCCCTG CAAGATGTGT AGCATTGTGC AGTTTGGGAA TATGGATGTG TGAGGAGCTT
    GTCAATCAAA CACATCATCC ACAAATCAAA GAAGCACTCA ATGTCATCTG TGTCTCCTTA
    AAATTTCCCA ATAAAGTGGT GGCCCAGGTC GCCTGCAGTG TGCTAAATGT GCTGCTCAAT
    TATGTTCAGC AGCTTCAGAT TTACCATTCA GACTACCCAC TTAAAATCAT TGAGATCCTG
    CTAGCGACAG TGACATATCT GCTGCCAAGT GCTGAGACCT CATCTTACGA GCAAGACAAG
    CGGTTGGTGG TCTCTCTGCT TCTTTCCCTT TTGGACTGGA TGATGGCATT ACCCCTTGCA
    TTGTTATTGA AACCACTACG CCATGCTCGG ACGGAGAGTG ATGGAAGGGA GAACTCTGTG
    TTAAACTGTG CATATAAGGT TCTACATGAC TGTGTCTATG GCTCCCAGCA TTTCAGCAAT
    CCCAGGTACT TTCCAATGAG CCTTTCAGAT CTGGCATCGG AGGATTATGA TCCCTTTATG
    CAACTGGAAT GCCTGAAGGA ACCAGAACCT TTACATTCCC CAGATTCTGA GCGCTCCACC
    AAACTGCAGC CTGTTACTGA GGTGAAAATT CCAAAGCAGC AAGGTCTTAT TCCAGTAGCA
    GCACGGACAG TAATCACACA TTTGGTAAAC CACTTGGGCC ACTATCCAAT GAGTGGAGAC
    CCCGCAATGT TGACTAGCCA GATCTGCGAG AACCATGATA ATCCATACTG TGAAAGTTCG
    GATCTATCTC CTCAACTCTT CCACAGCCCC AATCTGCAGT TCTTTGCTCT CAATAATACC
    ACTCTTGTGT CCTGCCTTCA GATACAGTCT GAAGAGAAAC TACCTCCTGG TGAGGACCTC
    TCTTCTGCCA GATCAACTGT ACGCATAATT ATGCGGGACC TTTCTGGCAA GTACAGTTGG
    GATTCCTCTA TATTATACGG ACCTCCTCTC CCATGTGAGG GACAAATGAC TCAGTGGTCT
    TCCCTTCTGG CAACATTTTC TCCTCCTTCC AATCAAGAGG TTGAACACCC ATCAACCCAG
    AGGGAATCTG AGGACTTTTC TTCTCTTTCT TCAAGAAGGC GAGGAAGGGA ATCTCTGCCC
    CTATGGGACA GCTTATCAGA TGAACAAGAT GCTCTTGACT GCCTTTTGCA GTATCTGGGA
    GCTACTGGTC CAGAGTGCCT TCAGAGGGCT GGTGTTCCCC TCAATGTGCC CTCACCTCCA
    CCTGCTTGTT TCTCTGAGAA GCTGGAGAAT GAGGTTAGCA ATGCAATTCT GAAACAGAGT
    GCAGAAGAAA AGGAATTTGT GGAAAAGCAT AGCAGTAATC TTCACATGAG AGCAATGCCT
    CAAGATCAGC CTGTAAACCA AAAACCCCAA TCAGCTTTCT ACTACTCCCG GCTGTTGCTT
    AGTATCCTTG GCATGAACTC CTGGGAGAAG AGGCGAAACT TCCACTTGCT TAAGAAGAAT
    GAGAAACTGT TACGTGAGCT GAAAAATTTG GACTCCAGGC AGTGCCGCGA AACCCACAAA
    ATTGCAGTTT TCTACGTTGC AGACGGCCAG GAGGACAAGC ATTCAATCCT ATCTAACACC
    GGTGGCAGTC AGGACTATGA GGATTTTGTG GCTGGTCTTG GATGGGAGGT CAATCTTTCC
    AATCACTGTG GCTTCCTGGG AGGACTTCAG AGGAACAAGA GCACTGGCTT TAGCACTCCA
    TATTTTGCAA CCTCAATAGT GGAGGTCATG TTCCATGTCT CCACAAGAAT GATTTCAGAC
    TCTGATGATT GTTTAACCAA AAAGCTTCGC CACCTTGGGA ATGATGAGGT GCACATTGTG
    TGGTCAGAAC ACAGTCGGGA ATATCGCCGC GGCATCATAC CCACTGAATT TGGGGACGTC
    CTACTTGTAA TATACCCCAT GAAGAACCAC ATGTTCAGCA TCCAGATTAT GAAGAAGCCA
    GAAATCCCAT TTTTTGGGCC CCTCTTCGAT GGAGCGATTG TGAATGGCAA AATCCTCCCA
    GGACTGGTTC GTGCAACAGC CATCAATGCC AGCAGAGCAC TTAAATCTCT TATCCCCCTG
    TACCAAAATT TCTTTGAGGA ACGGATGCGA TACTTGGATT CAATCACACA GCAGCATTTG
    GAACCCACCA CCTTTGAAGA CTACGCTGCC CAGGTGTTCT GCCCAGTGCC GCACAATCCC
    CTCCAGCTGA ATGCACGGCC TTGCCCAACG AAAGAGCAAG ATGAGAACGT ATCGCCTCCG
    GGGGATGGAT CAGAACTGGC TTTTTCTTTG TCACCACGGA GCAGCAAAAG TCGCATGTCC
    ATAAAGCTCC GGCGTTCCTC TGGCTCTGCC AACAAAACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746418.1
    CDS255..6596
    Translation

    Target ORF information:

    RefSeq Version XM_031890558.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGCTACTA AAGAAGATTT AGCTCCCCAA
    GTCAATGATG TTGCTGGTGC TGAAATTGCC ACTAATGCCA ATATCCCAGA CCTGATGGAT
    GAGTTTATAG CTGAGAGACT GCGATCAGGC ACCAGTGTGA CTATCACACG TAGGGGCAGC
    AGTTCTGGTA GTCTTGAGGT TCCCAAAGAT TTACCTGATA TGCTTAATAA GCATAGCCAG
    ATGCGCCCAG CTGATGACCC TGGTGTCCCT TCAGAGTGGA CCTCTCCGGC AAGCGCAGCC
    AGCAGTGACC TTCTTAGCTC AGACAGCCAT TCGGACTCTT TTAATGCCTT CCAGTATGAT
    GCAAGGAAGT TTGAAGCAGG TTTTCCATTT GGAGGAGATT CAACAGTCCC CATCTGTATT
    GAAGCAGATG CTGGCTCTAC ACATCTTAGC GTTGAGGAAC CAGAAGTTGG AAGTCTTACA
    GCACTGCATA TTGACTCTGA GACAAGCAGC TTCAATCAGC AGATTATGTG CACGGAGGCA
    GTGGCTGTAA CAGGGTCAGA AAGTGTTTCC CCTGCTTTGT CTGCTCATGG GTCGAGATCA
    GAGACTCCCT CTCCTGCAGC TCTAAACTCA GATCAGGCAG AGCTACACGA CAAGCTTCAG
    CATTCGGTTT TACAAACTCC CGATGATATA GAGCCTTGTG ACTTTCCTTT AGAAAGCTGC
    AGTGTTATGG CTGGAGGAAC ACTTACTGGA TGGCACGCGG ACGTTTCCAC AGTCATGTGG
    AGAAGAATGC TTGGGATTCT TGGGGATGTC AATAACATTG CCAATCCTGA AATTCATGCC
    CAAGTTTTTG ATTACTTGTG TGAACTCTGG CAGAACCTGG CTAAGATTAG AGATAATTTG
    GGAATATCCT TGGATAATCA GTCTTCTCCC CGACCTCCTG TTCTTATCCC ACCCCTGAGG
    ATTCTCACTC CCTGGCTTTT TAAGGCTACG ATGCTGCCTG AAAAATACAA GCAGGGCAAA
    CTCCATGCTT ACAAACTGAT TTGCAAGATA ATGAAGAGAA GACAGGACGT ATCTCCAAAC
    AGGGACTTCT TAACGCACTT CTATAACATT ATGCACTGTG GTCTTCTTCA CAACGATCAG
    GACATTGTGA ATACTATAAT CAAGCACTGT TCACCTCAAT TCTTTGCCCT TGGCCTTCCT
    GGTGCCACAC TCCTTATTAT GGATTTTATT GTAGCTGCTG GGAGAGTTGC TTCCTCGTCA
    TCCCTTAATG CTCCACGTGT GGAAGCTCAA GTTCTTCTGG GATCGCTTGT CTGTTTCCCC
    AACCTCTACC AGGAATTGCC TGCTTTGCAC CCAAATAGCT CTGACATAGT AATGTCACAT
    TTTACAGATG TCAAGGAACT CACAATTAAA ACTGTACTAA ATTCAGCCAG AGATGAACCG
    TCTGGCCCTG CAAGATGTGT AGCATTGTGC AGTTTGGGAA TATGGATGTG TGAGGAGCTT
    GTCAATCAAA CACATCATCC ACAAATCAAA GAAGCACTCA ATGTCATCTG TGTCTCCTTA
    AAATTTCCCA ATAAAGTGGT GGCCCAGGTC GCCTGCAGTG TGCTAAATGT GCTGCTCAAT
    TATGTTCAGC AGCTTCAGAT TTACCATTCA GACTACCCAC TTAAAATCAT TGAGATCCTG
    CTAGCGACAG TGACATATCT GCTGCCAAGT GCTGAGACCT CATCTTACGA GCAAGACAAG
    CGGTTGGTGG TCTCTCTGCT TCTTTCCCTT TTGGACTGGA TGATGGCATT ACCCCTTGCA
    TTGTTATTGA AACCACTACG CCATGCTCGG ACGGAGAGTG ATGGAAGGGA GAACTCTGTG
    TTAAACTGTG CATATAAGGT TCTACATGAC TGTGTCTATG GCTCCCAGCA TTTCAGCAAT
    CCCAGGTACT TTCCAATGAG CCTTTCAGAT CTGGCATCGG AGGATTATGA TCCCTTTATG
    CAACTGGAAT GCCTGAAGGA ACCAGAACCT TTACATTCCC CAGATTCTGA GCGCTCCACC
    AAACTGCAGC CTGTTACTGA GGTGAAAATT CCAAAGCAGC AAGGTCTTAT TCCAGTAGCA
    GCACGGACAG TAATCACACA TTTGGTAAAC CACTTGGGCC ACTATCCAAT GAGTGGAGAC
    CCCGCAATGT TGACTAGCCA GATCTGCGAG AACCATGATA ATCCATACTG TGAAAGTTCG
    GATCTATCTC CTCAACTCTT CCACAGCCCC AATCTGCAGT TCTTTGCTCT CAATAATACC
    ACTCTTGTGT CCTGCCTTCA GATACAGTCT GAAGAGAAAC TACCTCCTGG TGAGGACCTC
    TCTTCTGCCA GATCAACTGT ACGCATAATT ATGCGGGACC TTTCTGGCAA GTACAGTTGG
    GATTCCTCTA TATTATACGG ACCTCCTCTC CCATGTGAGG GACAAATGAC TCAGTGGTCT
    TCCCTTCTGG CAACATTTTC TCCTCCTTCC AATCAAGAGG TTGAACACCC ATCAACCCAG
    AGGGAATCTG AGGACTTTTC TTCTCTTTCT TCAAGAAGGC GAGGAAGGGA ATCTCTGCCC
    CTATGGGACA GCTTATCAGA TGAACAAGAT GCTCTTGACT GCCTTTTGCA GTATCTGGGA
    GCTACTGGTC CAGAGTGCCT TCAGAGGGCT GGTGTTCCCC TCAATGTGCC CTCACCTCCA
    CCTGCTTGTT TCTCTGAGAA GCTGGAGAAT GAGGTTAGCA ATGCAATTCT GAAACAGAGT
    GCAGAAGAAA AGGAATTTGT GGAAAAGCAT AGCAGTAATC TTCACATGAG AGCAATGCCT
    CAAGATCAGC CTGTAAACCA AAAACCCCAA TCAGCTTTCT ACTACTCCCG GCTGTTGCTT
    AGTATCCTTG GCATGAACTC CTGGGAGAAG AGGCGAAACT TCCACTTGCT TAAGAAGAAT
    GAGAAACTGT TACGTGAGCT GAAAAATTTG GACTCCAGGC AGTGCCGCGA AACCCACAAA
    ATTGCAGTTT TCTACGTTGC AGACGGCCAG GAGGACAAGC ATTCAATCCT ATCTAACACC
    GGTGGCAGTC AGGACTATGA GGATTTTGTG GCTGGTCTTG GATGGGAGGT CAATCTTTCC
    AATCACTGTG GCTTCCTGGG AGGACTTCAG AGGAACAAGA GCACTGGCTT TAGCACTCCA
    TATTTTGCAA CCTCAATAGT GGAGGTCATG TTCCATGTCT CCACAAGAAT GATTTCAGAC
    TCTGATGATT GTTTAACCAA AAAGCTTCGC CACCTTGGGA ATGATGAGGT GCACATTGTG
    TGGTCAGAAC ACAGTCGGGA ATATCGCCGC GGCATCATAC CCACTGAATT TGGGGACGTC
    CTACTTGTAA TATACCCCAT GAAGAACCAC ATGTTCAGCA TCCAGATTAT GAAGAAGCCA
    GAAATCCCAT TTTTTGGGCC CCTCTTCGAT GGAGCGATTG TGAATGGCAA AATCCTCCCA
    GGACTGGTTC GTGCAACAGC CATCAATGCC AGCAGAGCAC TTAAATCTCT TATCCCCCTG
    TACCAAAATT TCTTTGAGGA ACGGATGCGA TACTTGGATT CAATCACACA GCAGCATTTG
    GAACCCACCA CCTTTGAAGA CTACGCTGCC CAGGTGTTCT GCCCAGTGCC GCACAATCCC
    CTCCAGCTGA ATGCACGGCC TTGCCCAACG AAAGAGCAAG ATGAGAACGT ATCGCCTCCG
    GGGGATGGAT CAGAACTGGC TTTTTCTTTG TCACCACGGA GCAGCAAAAG TCGCATGTCC
    ATAAAGCTCC GGCGTTCCTC TGGCTCTGCC AACAAAACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63781
    Clone ID Related Accession (Same CDS sequence) XM_031890559.1
    Accession Version XM_031890559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGCTACTA AAGAAGATTT AGCTCCCCAA
    GTCAATGATG TTGCTGGTGC TGAAATTGCC ACTAATGCCA ATATCCCAGA CCTGATGGAT
    GAGTTTATAG CTGAGAGACT GCGATCAGGC ACCAGTGTGA CTATCACACG TAGGGGCAGC
    AGTTCTGGTA GTCTTGAGGT TCCCAAAGAT TTACCTGATA TGCTTAATAA GCATAGCCAG
    ATGCGCCCAG CTGATGACCC TGGTGTCCCT TCAGAGTGGA CCTCTCCGGC AAGCGCAGCC
    AGCAGTGACC TTCTTAGCTC AGACAGCCAT TCGGACTCTT TTAATGCCTT CCAGTATGAT
    GCAAGGAAGT TTGAAGGTTT TCCATTTGGA GGAGATTCAA CAGTCCCCAT CTGTATTGAA
    GCAGATGCTG GCTCTACACA TCTTAGCGTT GAGGAACCAG AAGTTGGAAG TCTTACAGCA
    CTGCATATTG ACTCTGAGAC AAGCAGCTTC AATCAGCAGA TTATGTGCAC GGAGGCAGTG
    GCTGTAACAG GGTCAGAAAG TGTTTCCCCT GCTTTGTCTG CTCATGGGTC GAGATCAGAG
    ACTCCCTCTC CTGCAGCTCT AAACTCAGAT CAGGCAGAGC TACACGACAA GCTTCAGCAT
    TCGGTTTTAC AAACTCCCGA TGATATAGAG CCTTGTGACT TTCCTTTAGA AAGCTGCAGT
    GTTATGGCTG GAGGAACACT TACTGGATGG CACGCGGACG TTTCCACAGT CATGTGGAGA
    AGAATGCTTG GGATTCTTGG GGATGTCAAT AACATTGCCA ATCCTGAAAT TCATGCCCAA
    GTTTTTGATT ACTTGTGTGA ACTCTGGCAG AACCTGGCTA AGATTAGAGA TAATTTGGGA
    ATATCCTTGG ATAATCAGTC TTCTCCCCGA CCTCCTGTTC TTATCCCACC CCTGAGGATT
    CTCACTCCCT GGCTTTTTAA GGCTACGATG CTGCCTGAAA AATACAAGCA GGGCAAACTC
    CATGCTTACA AACTGATTTG CAAGATAATG AAGAGAAGAC AGGACGTATC TCCAAACAGG
    GACTTCTTAA CGCACTTCTA TAACATTATG CACTGTGGTC TTCTTCACAA CGATCAGGAC
    ATTGTGAATA CTATAATCAA GCACTGTTCA CCTCAATTCT TTGCCCTTGG CCTTCCTGGT
    GCCACACTCC TTATTATGGA TTTTATTGTA GCTGCTGGGA GAGTTGCTTC CTCGTCATCC
    CTTAATGCTC CACGTGTGGA AGCTCAAGTT CTTCTGGGAT CGCTTGTCTG TTTCCCCAAC
    CTCTACCAGG AATTGCCTGC TTTGCACCCA AATAGCTCTG ACATAGTAAT GTCACATTTT
    ACAGATGTCA AGGAACTCAC AATTAAAACT GTACTAAATT CAGCCAGAGA TGAACCGTCT
    GGCCCTGCAA GATGTGTAGC ATTGTGCAGT TTGGGAATAT GGATGTGTGA GGAGCTTGTC
    AATCAAACAC ATCATCCACA AATCAAAGAA GCACTCAATG TCATCTGTGT CTCCTTAAAA
    TTTCCCAATA AAGTGGTGGC CCAGGTCGCC TGCAGTGTGC TAAATGTGCT GCTCAATTAT
    GTTCAGCAGC TTCAGATTTA CCATTCAGAC TACCCACTTA AAATCATTGA GATCCTGCTA
    GCGACAGTGA CATATCTGCT GCCAAGTGCT GAGACCTCAT CTTACGAGCA AGACAAGCGG
    TTGGTGGTCT CTCTGCTTCT TTCCCTTTTG GACTGGATGA TGGCATTACC CCTTGCATTG
    TTATTGAAAC CACTACGCCA TGCTCGGACG GAGAGTGATG GAAGGGAGAA CTCTGTGTTA
    AACTGTGCAT ATAAGGTTCT ACATGACTGT GTCTATGGCT CCCAGCATTT CAGCAATCCC
    AGGTACTTTC CAATGAGCCT TTCAGATCTG GCATCGGAGG ATTATGATCC CTTTATGCAA
    CTGGAATGCC TGAAGGAACC AGAACCTTTA CATTCCCCAG ATTCTGAGCG CTCCACCAAA
    CTGCAGCCTG TTACTGAGGT GAAAATTCCA AAGCAGCAAG GTCTTATTCC AGTAGCAGCA
    CGGACAGTAA TCACACATTT GGTAAACCAC TTGGGCCACT ATCCAATGAG TGGAGACCCC
    GCAATGTTGA CTAGCCAGAT CTGCGAGAAC CATGATAATC CATACTGTGA AAGTTCGGAT
    CTATCTCCTC AACTCTTCCA CAGCCCCAAT CTGCAGTTCT TTGCTCTCAA TAATACCACT
    CTTGTGTCCT GCCTTCAGAT ACAGTCTGAA GAGAAACTAC CTCCTGGTGA GGACCTCTCT
    TCTGCCAGAT CAACTGTACG CATAATTATG CGGGACCTTT CTGGCAAGTA CAGTTGGGAT
    TCCTCTATAT TATACGGACC TCCTCTCCCA TGTGAGGGAC AAATGACTCA GTGGTCTTCC
    CTTCTGGCAA CATTTTCTCC TCCTTCCAAT CAAGAGGTTG AACACCCATC AACCCAGAGG
    GAATCTGAGG ACTTTTCTTC TCTTTCTTCA AGAAGGCGAG GAAGGGAATC TCTGCCCCTA
    TGGGACAGCT TATCAGATGA ACAAGATGCT CTTGACTGCC TTTTGCAGTA TCTGGGAGCT
    ACTGGTCCAG AGTGCCTTCA GAGGGCTGGT GTTCCCCTCA ATGTGCCCTC ACCTCCACCT
    GCTTGTTTCT CTGAGAAGCT GGAGAATGAG GTTAGCAATG CAATTCTGAA ACAGAGTGCA
    GAAGAAAAGG AATTTGTGGA AAAGCATAGC AGTAATCTTC ACATGAGAGC AATGCCTCAA
    GATCAGCCTG TAAACCAAAA ACCCCAATCA GCTTTCTACT ACTCCCGGCT GTTGCTTAGT
    ATCCTTGGCA TGAACTCCTG GGAGAAGAGG CGAAACTTCC ACTTGCTTAA GAAGAATGAG
    AAACTGTTAC GTGAGCTGAA AAATTTGGAC TCCAGGCAGT GCCGCGAAAC CCACAAAATT
    GCAGTTTTCT ACGTTGCAGA CGGCCAGGAG GACAAGCATT CAATCCTATC TAACACCGGT
    GGCAGTCAGG ACTATGAGGA TTTTGTGGCT GGTCTTGGAT GGGAGGTCAA TCTTTCCAAT
    CACTGTGGCT TCCTGGGAGG ACTTCAGAGG AACAAGAGCA CTGGCTTTAG CACTCCATAT
    TTTGCAACCT CAATAGTGGA GGTCATGTTC CATGTCTCCA CAAGAATGAT TTCAGACTCT
    GATGATTGTT TAACCAAAAA GCTTCGCCAC CTTGGGAATG ATGAGGTGCA CATTGTGTGG
    TCAGAACACA GTCGGGAATA TCGCCGCGGC ATCATACCCA CTGAATTTGG GGACGTCCTA
    CTTGTAATAT ACCCCATGAA GAACCACATG TTCAGCATCC AGATTATGAA GAAGCCAGAA
    ATCCCATTTT TTGGGCCCCT CTTCGATGGA GCGATTGTGA ATGGCAAAAT CCTCCCAGGA
    CTGGTTCGTG CAACAGCCAT CAATGCCAGC AGAGCACTTA AATCTCTTAT CCCCCTGTAC
    CAAAATTTCT TTGAGGAACG GATGCGATAC TTGGATTCAA TCACACAGCA GCATTTGGAA
    CCCACCACCT TTGAAGACTA CGCTGCCCAG GTGTTCTGCC CAGTGCCGCA CAATCCCCTC
    CAGCTGAATG CACGGCCTTG CCCAACGAAA GAGCAAGATG AGAACGTATC GCCTCCGGGG
    GATGGATCAG AACTGGCTTT TTCTTTGTCA CCACGGAGCA GCAAAAGTCG CATGTCCATA
    AAGCTCCGGC GTTCCTCTGG CTCTGCCAAC AAAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746419.1
    CDS255..6593
    Translation

    Target ORF information:

    RefSeq Version XM_031890559.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGCCATGAGG AGCCGATCCA TTGGGGACAG TGCCTTGCCA
    TCCACTTCTA TCCGCAGCGC TAAAAGTGCA CCTGTCCTGA TCCATCCTTC CAAACCCCTT
    CTGCCTGATA TTGTTCTCAC TCTGCTTTCC GATGAGCTGT CAGATATGGA TGATGCTCCT
    ATGCATCCAC GACCATCACG AGTGAGGCAC TATTCTCAGA GTGAGGAGAC GCCTACAGAA
    ACATTTGCAG CAGTAAATGA AGAAGCACCA ACTCTACCTC GCAGCAGCAG CACCTCTGAC
    ATCCTGGGCC CCTTCACAGC TGAAAGAACC AAAGCTACTA AAGAAGATTT AGCTCCCCAA
    GTCAATGATG TTGCTGGTGC TGAAATTGCC ACTAATGCCA ATATCCCAGA CCTGATGGAT
    GAGTTTATAG CTGAGAGACT GCGATCAGGC ACCAGTGTGA CTATCACACG TAGGGGCAGC
    AGTTCTGGTA GTCTTGAGGT TCCCAAAGAT TTACCTGATA TGCTTAATAA GCATAGCCAG
    ATGCGCCCAG CTGATGACCC TGGTGTCCCT TCAGAGTGGA CCTCTCCGGC AAGCGCAGCC
    AGCAGTGACC TTCTTAGCTC AGACAGCCAT TCGGACTCTT TTAATGCCTT CCAGTATGAT
    GCAAGGAAGT TTGAAGGTTT TCCATTTGGA GGAGATTCAA CAGTCCCCAT CTGTATTGAA
    GCAGATGCTG GCTCTACACA TCTTAGCGTT GAGGAACCAG AAGTTGGAAG TCTTACAGCA
    CTGCATATTG ACTCTGAGAC AAGCAGCTTC AATCAGCAGA TTATGTGCAC GGAGGCAGTG
    GCTGTAACAG GGTCAGAAAG TGTTTCCCCT GCTTTGTCTG CTCATGGGTC GAGATCAGAG
    ACTCCCTCTC CTGCAGCTCT AAACTCAGAT CAGGCAGAGC TACACGACAA GCTTCAGCAT
    TCGGTTTTAC AAACTCCCGA TGATATAGAG CCTTGTGACT TTCCTTTAGA AAGCTGCAGT
    GTTATGGCTG GAGGAACACT TACTGGATGG CACGCGGACG TTTCCACAGT CATGTGGAGA
    AGAATGCTTG GGATTCTTGG GGATGTCAAT AACATTGCCA ATCCTGAAAT TCATGCCCAA
    GTTTTTGATT ACTTGTGTGA ACTCTGGCAG AACCTGGCTA AGATTAGAGA TAATTTGGGA
    ATATCCTTGG ATAATCAGTC TTCTCCCCGA CCTCCTGTTC TTATCCCACC CCTGAGGATT
    CTCACTCCCT GGCTTTTTAA GGCTACGATG CTGCCTGAAA AATACAAGCA GGGCAAACTC
    CATGCTTACA AACTGATTTG CAAGATAATG AAGAGAAGAC AGGACGTATC TCCAAACAGG
    GACTTCTTAA CGCACTTCTA TAACATTATG CACTGTGGTC TTCTTCACAA CGATCAGGAC
    ATTGTGAATA CTATAATCAA GCACTGTTCA CCTCAATTCT TTGCCCTTGG CCTTCCTGGT
    GCCACACTCC TTATTATGGA TTTTATTGTA GCTGCTGGGA GAGTTGCTTC CTCGTCATCC
    CTTAATGCTC CACGTGTGGA AGCTCAAGTT CTTCTGGGAT CGCTTGTCTG TTTCCCCAAC
    CTCTACCAGG AATTGCCTGC TTTGCACCCA AATAGCTCTG ACATAGTAAT GTCACATTTT
    ACAGATGTCA AGGAACTCAC AATTAAAACT GTACTAAATT CAGCCAGAGA TGAACCGTCT
    GGCCCTGCAA GATGTGTAGC ATTGTGCAGT TTGGGAATAT GGATGTGTGA GGAGCTTGTC
    AATCAAACAC ATCATCCACA AATCAAAGAA GCACTCAATG TCATCTGTGT CTCCTTAAAA
    TTTCCCAATA AAGTGGTGGC CCAGGTCGCC TGCAGTGTGC TAAATGTGCT GCTCAATTAT
    GTTCAGCAGC TTCAGATTTA CCATTCAGAC TACCCACTTA AAATCATTGA GATCCTGCTA
    GCGACAGTGA CATATCTGCT GCCAAGTGCT GAGACCTCAT CTTACGAGCA AGACAAGCGG
    TTGGTGGTCT CTCTGCTTCT TTCCCTTTTG GACTGGATGA TGGCATTACC CCTTGCATTG
    TTATTGAAAC CACTACGCCA TGCTCGGACG GAGAGTGATG GAAGGGAGAA CTCTGTGTTA
    AACTGTGCAT ATAAGGTTCT ACATGACTGT GTCTATGGCT CCCAGCATTT CAGCAATCCC
    AGGTACTTTC CAATGAGCCT TTCAGATCTG GCATCGGAGG ATTATGATCC CTTTATGCAA
    CTGGAATGCC TGAAGGAACC AGAACCTTTA CATTCCCCAG ATTCTGAGCG CTCCACCAAA
    CTGCAGCCTG TTACTGAGGT GAAAATTCCA AAGCAGCAAG GTCTTATTCC AGTAGCAGCA
    CGGACAGTAA TCACACATTT GGTAAACCAC TTGGGCCACT ATCCAATGAG TGGAGACCCC
    GCAATGTTGA CTAGCCAGAT CTGCGAGAAC CATGATAATC CATACTGTGA AAGTTCGGAT
    CTATCTCCTC AACTCTTCCA CAGCCCCAAT CTGCAGTTCT TTGCTCTCAA TAATACCACT
    CTTGTGTCCT GCCTTCAGAT ACAGTCTGAA GAGAAACTAC CTCCTGGTGA GGACCTCTCT
    TCTGCCAGAT CAACTGTACG CATAATTATG CGGGACCTTT CTGGCAAGTA CAGTTGGGAT
    TCCTCTATAT TATACGGACC TCCTCTCCCA TGTGAGGGAC AAATGACTCA GTGGTCTTCC
    CTTCTGGCAA CATTTTCTCC TCCTTCCAAT CAAGAGGTTG AACACCCATC AACCCAGAGG
    GAATCTGAGG ACTTTTCTTC TCTTTCTTCA AGAAGGCGAG GAAGGGAATC TCTGCCCCTA
    TGGGACAGCT TATCAGATGA ACAAGATGCT CTTGACTGCC TTTTGCAGTA TCTGGGAGCT
    ACTGGTCCAG AGTGCCTTCA GAGGGCTGGT GTTCCCCTCA ATGTGCCCTC ACCTCCACCT
    GCTTGTTTCT CTGAGAAGCT GGAGAATGAG GTTAGCAATG CAATTCTGAA ACAGAGTGCA
    GAAGAAAAGG AATTTGTGGA AAAGCATAGC AGTAATCTTC ACATGAGAGC AATGCCTCAA
    GATCAGCCTG TAAACCAAAA ACCCCAATCA GCTTTCTACT ACTCCCGGCT GTTGCTTAGT
    ATCCTTGGCA TGAACTCCTG GGAGAAGAGG CGAAACTTCC ACTTGCTTAA GAAGAATGAG
    AAACTGTTAC GTGAGCTGAA AAATTTGGAC TCCAGGCAGT GCCGCGAAAC CCACAAAATT
    GCAGTTTTCT ACGTTGCAGA CGGCCAGGAG GACAAGCATT CAATCCTATC TAACACCGGT
    GGCAGTCAGG ACTATGAGGA TTTTGTGGCT GGTCTTGGAT GGGAGGTCAA TCTTTCCAAT
    CACTGTGGCT TCCTGGGAGG ACTTCAGAGG AACAAGAGCA CTGGCTTTAG CACTCCATAT
    TTTGCAACCT CAATAGTGGA GGTCATGTTC CATGTCTCCA CAAGAATGAT TTCAGACTCT
    GATGATTGTT TAACCAAAAA GCTTCGCCAC CTTGGGAATG ATGAGGTGCA CATTGTGTGG
    TCAGAACACA GTCGGGAATA TCGCCGCGGC ATCATACCCA CTGAATTTGG GGACGTCCTA
    CTTGTAATAT ACCCCATGAA GAACCACATG TTCAGCATCC AGATTATGAA GAAGCCAGAA
    ATCCCATTTT TTGGGCCCCT CTTCGATGGA GCGATTGTGA ATGGCAAAAT CCTCCCAGGA
    CTGGTTCGTG CAACAGCCAT CAATGCCAGC AGAGCACTTA AATCTCTTAT CCCCCTGTAC
    CAAAATTTCT TTGAGGAACG GATGCGATAC TTGGATTCAA TCACACAGCA GCATTTGGAA
    CCCACCACCT TTGAAGACTA CGCTGCCCAG GTGTTCTGCC CAGTGCCGCA CAATCCCCTC
    CAGCTGAATG CACGGCCTTG CCCAACGAAA GAGCAAGATG AGAACGTATC GCCTCCGGGG
    GATGGATCAG AACTGGCTTT TTCTTTGTCA CCACGGAGCA GCAAAAGTCG CATGTCCATA
    AAGCTCCGGC GTTCCTCTGG CTCTGCCAAC AAAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63782
    Clone ID Related Accession (Same CDS sequence) XM_031890560.1
    Accession Version XM_031890560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGCTACTAA AGAAGATTTA GCTCCCCAAG TCAATGATGT TGCTGGTGCT
    GAAATTGCCA CTAATGCCAA TATCCCAGAC CTGATGGATG AGTTTATAGC TGAGAGACTG
    CGATCAGGCA CCAGTGTGAC TATCACACGT AGGGGCAGCA GTTCTGGTAG TCTTGAGGTT
    CCCAAAGATT TACCTGATAT GCTTAATAAG CATAGCCAGA TGCGCCCAGC TGATGACCCT
    GGTGTCCCTT CAGAGTGGAC CTCTCCGGCA AGCGCAGCCA GCAGTGACCT TCTTAGCTCA
    GACAGCCATT CGGACTCTTT TAATGCCTTC CAGTATGATG CAAGGAAGTT TGAAGCAGGT
    TTTCCATTTG GAGGAGATTC AACAGTCCCC ATCTGTATTG AAGCAGATGC TGGCTCTACA
    CATCTTAGCG TTGAGGAACC AGAAGTTGGA AGTCTTACAG CACTGCATAT TGACTCTGAG
    ACAAGCAGCT TCAATCAGCA GATTATGTGC ACGGAGGCAG TGGCTGTAAC AGGGTCAGAA
    AGTGTTTCCC CTGCTTTGTC TGCTCATGGG TCGAGATCAG AGACTCCCTC TCCTGCAGCT
    CTAAACTCAG ATCAGGCAGA GCTACACGAC AAGCTTCAGC ATTCGGTTTT ACAAACTCCC
    GATGATATAG AGCCTTGTGA CTTTCCTTTA GAAAGCTGCA GTGTTATGGC TGGAGGAACA
    CTTACTGGAT GGCACGCGGA CGTTTCCACA GTCATGTGGA GAAGAATGCT TGGGATTCTT
    GGGGATGTCA ATAACATTGC CAATCCTGAA ATTCATGCCC AAGTTTTTGA TTACTTGTGT
    GAACTCTGGC AGAACCTGGC TAAGATTAGA GATAATTTGG GAATATCCTT GGATAATCAG
    TCTTCTCCCC GACCTCCTGT TCTTATCCCA CCCCTGAGGA TTCTCACTCC CTGGCTTTTT
    AAGGCTACGA TGCTGCCTGA AAAATACAAG CAGGGCAAAC TCCATGCTTA CAAACTGATT
    TGCAAGATAA TGAAGAGAAG ACAGGACGTA TCTCCAAACA GGGACTTCTT AACGCACTTC
    TATAACATTA TGCACTGTGG TCTTCTTCAC AACGATCAGG ACATTGTGAA TACTATAATC
    AAGCACTGTT CACCTCAATT CTTTGCCCTT GGCCTTCCTG GTGCCACACT CCTTATTATG
    GATTTTATTG TAGCTGCTGG GAGAGTTGCT TCCTCGTCAT CCCTTAATGC TCCACGTGTG
    GAAGCTCAAG TTCTTCTGGG ATCGCTTGTC TGTTTCCCCA ACCTCTACCA GGAATTGCCT
    GCTTTGCACC CAAATAGCTC TGACATAGTA ATGTCACATT TTACAGATGT CAAGGAACTC
    ACAATTAAAA CTGTACTAAA TTCAGCCAGA GATGAACCGT CTGGCCCTGC AAGATGTGTA
    GCATTGTGCA GTTTGGGAAT ATGGATGTGT GAGGAGCTTG TCAATCAAAC ACATCATCCA
    CAAATCAAAG AAGCACTCAA TGTCATCTGT GTCTCCTTAA AATTTCCCAA TAAAGTGGTG
    GCCCAGGTCG CCTGCAGTGT GCTAAATGTG CTGCTCAATT ATGTTCAGCA GCTTCAGATT
    TACCATTCAG ACTACCCACT TAAAATCATT GAGATCCTGC TAGCGACAGT GACATATCTG
    CTGCCAAGTG CTGAGACCTC ATCTTACGAG CAAGACAAGC GGTTGGTGGT CTCTCTGCTT
    CTTTCCCTTT TGGACTGGAT GATGGCATTA CCCCTTGCAT TGTTATTGAA ACCACTACGC
    CATGCTCGGA CGGAGAGTGA TGGAAGGGAG AACTCTGTGT TAAACTGTGC ATATAAGGTT
    CTACATGACT GTGTCTATGG CTCCCAGCAT TTCAGCAATC CCAGGTACTT TCCAATGAGC
    CTTTCAGATC TGGCATCGGA GGATTATGAT CCCTTTATGC AACTGGAATG CCTGAAGGAA
    CCAGAACCTT TACATTCCCC AGATTCTGAG CGCTCCACCA AACTGCAGCC TGTTACTGAG
    GTGAAAATTC CAAAGCAGCA AGGTCTTATT CCAGTAGCAG CACGGACAGT AATCACACAT
    TTGGTAAACC ACTTGGGCCA CTATCCAATG AGTGGAGACC CCGCAATGTT GACTAGCCAG
    ATCTGCGAGA ACCATGATAA TCCATACTGT GAAAGTTCGG ATCTATCTCC TCAACTCTTC
    CACAGCCCCA ATCTGCAGTT CTTTGCTCTC AATAATACCA CTCTTGTGTC CTGCCTTCAG
    ATACAGTCTG AAGAGAAACT ACCTCCTGGT GAGGACCTCT CTTCTGCCAG ATCAACTGTA
    CGCATAATTA TGCGGGACCT TTCTGGCAAG TACAGTTGGG ATTCCTCTAT ATTATACGGA
    CCTCCTCTCC CATGTGAGGG ACAAATGACT CAGTGGTCTT CCCTTCTGGC AACATTTTCT
    CCTCCTTCCA ATCAAGAGGT TGAACACCCA TCAACCCAGA GGGAATCTGA GGACTTTTCT
    TCTCTTTCTT CAAGAAGGCG AGGAAGGGAA TCTCTGCCCC TATGGGACAG CTTATCAGAT
    GAACAAGATG CTCTTGACTG CCTTTTGCAG TATCTGGGAG CTACTGGTCC AGAGTGCCTT
    CAGAGGGCTG GTGTTCCCCT CAATGTGCCC TCACCTCCAC CTGCTTGTTT CTCTGAGAAG
    CTGGAGAATG AGGTTAGCAA TGCAATTCTG AAACAGAGTG CAGAAGAAAA GGAATTTGTG
    GAAAAGCATA GCAGTAATCT TCACATGAGA GCAATGCCTC AAGATCAGCC TGTAAACCAA
    AAACCCCAAT CAGCTTTCTA CTACTCCCGG CTGTTGCTTA GTATCCTTGG CATGAACTCC
    TGGGAGAAGA GGCGAAACTT CCACTTGCTT AAGAAGAATG AGAAACTGTT ACGTGAGCTG
    AAAAATTTGG ACTCCAGGCA GTGCCGCGAA ACCCACAAAA TTGCAGTTTT CTACGTTGCA
    GACGGCCAGG AGGACAAGCA TTCAATCCTA TCTAACACCG GTGGCAGTCA GGACTATGAG
    GATTTTGTGG CTGGTCTTGG ATGGGAGGTC AATCTTTCCA ATCACTGTGG CTTCCTGGGA
    GGACTTCAGA GGAACAAGAG CACTGGCTTT AGCACTCCAT ATTTTGCAAC CTCAATAGTG
    GAGGTCATGT TCCATGTCTC CACAAGAATG ATTTCAGACT CTGATGATTG TTTAACCAAA
    AAGCTTCGCC ACCTTGGGAA TGATGAGGTG CACATTGTGT GGTCAGAACA CAGTCGGGAA
    TATCGCCGCG GCATCATACC CACTGAATTT GGGGACGTCC TACTTGTAAT ATACCCCATG
    AAGAACCACA TGTTCAGCAT CCAGATTATG AAGAAGCCAG AAATCCCATT TTTTGGGCCC
    CTCTTCGATG GAGCGATTGT GAATGGCAAA ATCCTCCCAG GACTGGTTCG TGCAACAGCC
    ATCAATGCCA GCAGAGCACT TAAATCTCTT ATCCCCCTGT ACCAAAATTT CTTTGAGGAA
    CGGATGCGAT ACTTGGATTC AATCACACAG CAGCATTTGG AACCCACCAC CTTTGAAGAC
    TACGCTGCCC AGGTGTTCTG CCCAGTGCCG CACAATCCCC TCCAGCTGAA TGCACGGCCT
    TGCCCAACGA AAGAGCAAGA TGAGAACGTA TCGCCTCCGG GGGATGGATC AGAACTGGCT
    TTTTCTTTGT CACCACGGAG CAGCAAAAGT CGCATGTCCA TAAAGCTCCG GCGTTCCTCT
    GGCTCTGCCA ACAAAACCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746420.1
    CDS254..6454
    Translation

    Target ORF information:

    RefSeq Version XM_031890560.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGCTACTAA AGAAGATTTA GCTCCCCAAG TCAATGATGT TGCTGGTGCT
    GAAATTGCCA CTAATGCCAA TATCCCAGAC CTGATGGATG AGTTTATAGC TGAGAGACTG
    CGATCAGGCA CCAGTGTGAC TATCACACGT AGGGGCAGCA GTTCTGGTAG TCTTGAGGTT
    CCCAAAGATT TACCTGATAT GCTTAATAAG CATAGCCAGA TGCGCCCAGC TGATGACCCT
    GGTGTCCCTT CAGAGTGGAC CTCTCCGGCA AGCGCAGCCA GCAGTGACCT TCTTAGCTCA
    GACAGCCATT CGGACTCTTT TAATGCCTTC CAGTATGATG CAAGGAAGTT TGAAGCAGGT
    TTTCCATTTG GAGGAGATTC AACAGTCCCC ATCTGTATTG AAGCAGATGC TGGCTCTACA
    CATCTTAGCG TTGAGGAACC AGAAGTTGGA AGTCTTACAG CACTGCATAT TGACTCTGAG
    ACAAGCAGCT TCAATCAGCA GATTATGTGC ACGGAGGCAG TGGCTGTAAC AGGGTCAGAA
    AGTGTTTCCC CTGCTTTGTC TGCTCATGGG TCGAGATCAG AGACTCCCTC TCCTGCAGCT
    CTAAACTCAG ATCAGGCAGA GCTACACGAC AAGCTTCAGC ATTCGGTTTT ACAAACTCCC
    GATGATATAG AGCCTTGTGA CTTTCCTTTA GAAAGCTGCA GTGTTATGGC TGGAGGAACA
    CTTACTGGAT GGCACGCGGA CGTTTCCACA GTCATGTGGA GAAGAATGCT TGGGATTCTT
    GGGGATGTCA ATAACATTGC CAATCCTGAA ATTCATGCCC AAGTTTTTGA TTACTTGTGT
    GAACTCTGGC AGAACCTGGC TAAGATTAGA GATAATTTGG GAATATCCTT GGATAATCAG
    TCTTCTCCCC GACCTCCTGT TCTTATCCCA CCCCTGAGGA TTCTCACTCC CTGGCTTTTT
    AAGGCTACGA TGCTGCCTGA AAAATACAAG CAGGGCAAAC TCCATGCTTA CAAACTGATT
    TGCAAGATAA TGAAGAGAAG ACAGGACGTA TCTCCAAACA GGGACTTCTT AACGCACTTC
    TATAACATTA TGCACTGTGG TCTTCTTCAC AACGATCAGG ACATTGTGAA TACTATAATC
    AAGCACTGTT CACCTCAATT CTTTGCCCTT GGCCTTCCTG GTGCCACACT CCTTATTATG
    GATTTTATTG TAGCTGCTGG GAGAGTTGCT TCCTCGTCAT CCCTTAATGC TCCACGTGTG
    GAAGCTCAAG TTCTTCTGGG ATCGCTTGTC TGTTTCCCCA ACCTCTACCA GGAATTGCCT
    GCTTTGCACC CAAATAGCTC TGACATAGTA ATGTCACATT TTACAGATGT CAAGGAACTC
    ACAATTAAAA CTGTACTAAA TTCAGCCAGA GATGAACCGT CTGGCCCTGC AAGATGTGTA
    GCATTGTGCA GTTTGGGAAT ATGGATGTGT GAGGAGCTTG TCAATCAAAC ACATCATCCA
    CAAATCAAAG AAGCACTCAA TGTCATCTGT GTCTCCTTAA AATTTCCCAA TAAAGTGGTG
    GCCCAGGTCG CCTGCAGTGT GCTAAATGTG CTGCTCAATT ATGTTCAGCA GCTTCAGATT
    TACCATTCAG ACTACCCACT TAAAATCATT GAGATCCTGC TAGCGACAGT GACATATCTG
    CTGCCAAGTG CTGAGACCTC ATCTTACGAG CAAGACAAGC GGTTGGTGGT CTCTCTGCTT
    CTTTCCCTTT TGGACTGGAT GATGGCATTA CCCCTTGCAT TGTTATTGAA ACCACTACGC
    CATGCTCGGA CGGAGAGTGA TGGAAGGGAG AACTCTGTGT TAAACTGTGC ATATAAGGTT
    CTACATGACT GTGTCTATGG CTCCCAGCAT TTCAGCAATC CCAGGTACTT TCCAATGAGC
    CTTTCAGATC TGGCATCGGA GGATTATGAT CCCTTTATGC AACTGGAATG CCTGAAGGAA
    CCAGAACCTT TACATTCCCC AGATTCTGAG CGCTCCACCA AACTGCAGCC TGTTACTGAG
    GTGAAAATTC CAAAGCAGCA AGGTCTTATT CCAGTAGCAG CACGGACAGT AATCACACAT
    TTGGTAAACC ACTTGGGCCA CTATCCAATG AGTGGAGACC CCGCAATGTT GACTAGCCAG
    ATCTGCGAGA ACCATGATAA TCCATACTGT GAAAGTTCGG ATCTATCTCC TCAACTCTTC
    CACAGCCCCA ATCTGCAGTT CTTTGCTCTC AATAATACCA CTCTTGTGTC CTGCCTTCAG
    ATACAGTCTG AAGAGAAACT ACCTCCTGGT GAGGACCTCT CTTCTGCCAG ATCAACTGTA
    CGCATAATTA TGCGGGACCT TTCTGGCAAG TACAGTTGGG ATTCCTCTAT ATTATACGGA
    CCTCCTCTCC CATGTGAGGG ACAAATGACT CAGTGGTCTT CCCTTCTGGC AACATTTTCT
    CCTCCTTCCA ATCAAGAGGT TGAACACCCA TCAACCCAGA GGGAATCTGA GGACTTTTCT
    TCTCTTTCTT CAAGAAGGCG AGGAAGGGAA TCTCTGCCCC TATGGGACAG CTTATCAGAT
    GAACAAGATG CTCTTGACTG CCTTTTGCAG TATCTGGGAG CTACTGGTCC AGAGTGCCTT
    CAGAGGGCTG GTGTTCCCCT CAATGTGCCC TCACCTCCAC CTGCTTGTTT CTCTGAGAAG
    CTGGAGAATG AGGTTAGCAA TGCAATTCTG AAACAGAGTG CAGAAGAAAA GGAATTTGTG
    GAAAAGCATA GCAGTAATCT TCACATGAGA GCAATGCCTC AAGATCAGCC TGTAAACCAA
    AAACCCCAAT CAGCTTTCTA CTACTCCCGG CTGTTGCTTA GTATCCTTGG CATGAACTCC
    TGGGAGAAGA GGCGAAACTT CCACTTGCTT AAGAAGAATG AGAAACTGTT ACGTGAGCTG
    AAAAATTTGG ACTCCAGGCA GTGCCGCGAA ACCCACAAAA TTGCAGTTTT CTACGTTGCA
    GACGGCCAGG AGGACAAGCA TTCAATCCTA TCTAACACCG GTGGCAGTCA GGACTATGAG
    GATTTTGTGG CTGGTCTTGG ATGGGAGGTC AATCTTTCCA ATCACTGTGG CTTCCTGGGA
    GGACTTCAGA GGAACAAGAG CACTGGCTTT AGCACTCCAT ATTTTGCAAC CTCAATAGTG
    GAGGTCATGT TCCATGTCTC CACAAGAATG ATTTCAGACT CTGATGATTG TTTAACCAAA
    AAGCTTCGCC ACCTTGGGAA TGATGAGGTG CACATTGTGT GGTCAGAACA CAGTCGGGAA
    TATCGCCGCG GCATCATACC CACTGAATTT GGGGACGTCC TACTTGTAAT ATACCCCATG
    AAGAACCACA TGTTCAGCAT CCAGATTATG AAGAAGCCAG AAATCCCATT TTTTGGGCCC
    CTCTTCGATG GAGCGATTGT GAATGGCAAA ATCCTCCCAG GACTGGTTCG TGCAACAGCC
    ATCAATGCCA GCAGAGCACT TAAATCTCTT ATCCCCCTGT ACCAAAATTT CTTTGAGGAA
    CGGATGCGAT ACTTGGATTC AATCACACAG CAGCATTTGG AACCCACCAC CTTTGAAGAC
    TACGCTGCCC AGGTGTTCTG CCCAGTGCCG CACAATCCCC TCCAGCTGAA TGCACGGCCT
    TGCCCAACGA AAGAGCAAGA TGAGAACGTA TCGCCTCCGG GGGATGGATC AGAACTGGCT
    TTTTCTTTGT CACCACGGAG CAGCAAAAGT CGCATGTCCA TAAAGCTCCG GCGTTCCTCT
    GGCTCTGCCA ACAAAACCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63783
    Clone ID Related Accession (Same CDS sequence) XM_031890561.1
    Accession Version XM_031890561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ral GTPase-activating protein subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGGTGTGGT GTCTGGTACA GATCATCTCA CCCCCCAGGC TTTGGACCCC
    CCCTCTTCTA ACACACAGTC TCAGGAAGCA CCCATTACGC AGGTCGATGG CAGCATATAT
    GATCATCTCT GCCAGCTTGC TGGCTCAGCT GAATTAAGAG ATGGAATAAA GGAACAGGGC
    TTGTACGAGG AATTAGAGGG TGAAGGTCTG TTGCCACGTT TGAGAGCCTA TTTAAGGGAG
    CAGGAAGTTC TAGAAGTACT GAAAGACAGG ACGGGCAACT ACCTGTTTCA TCAAGACAGC
    GAGCATTCTG AACATGTAGA TTGTTATAAT AAAGGGGAAG TCAGTTCATT CATTCCTGAT
    TTATACAGTC AAGTAGTTTC TTCAGAGCAG AATGATAACT GTTTAGGTGG GGTGGTTAAT
    ATAGCCAAGA AAGCTGGTTG CATTAGGATG AGCAACAGGT CACGAGAAGT CCATGTAAGG
    ATACAGGTTG ATGAAGAAGA AAGGGAGAAT GATTATGTTG CCCAGGAGCA AGGTGAGCGA
    AAGAGGGGCC TCTATAGGCA ACAAGCAGCT GAGGGAGAGG AAACCTCTGA CTTGGCAACT
    TCAAAGTTTC ACCACTCCTC TGGCACTGAA CATGGGGCCA ACATAAAGAA TCTTTGTGAG
    TCTGTAACAC ACAAGGCTAA AGCAATGCGC CGGAAACGCA GCAGTGTTCA GGTTACCTTC
    CGTCCAGCCA CTGAGGCTGT GCTTTTCTAC AACTCTATGG AAAACAAAGA TGCCCCTTGG
    AAAGTGAGGC TGCGTAAATT AAGTGGTTTA AGCACTAACC CCTCTATAGC TGTAGAGCCA
    GGAAAATCCA CTGCCTATGC TGCACTTGGT GCAGTCAGAG TAGCATCAAG TGTTAGCCAG
    CCAGGTAATG ACCACCATGA TTTACCTTAC CCTCCCCCAC AAATCCAAAA GGAATGTGCC
    CGAATAAGGC AGAGATCCTG CAGTCAGGAG AGAGCCCCCT CTCATGCGTC ATTGGGTGGC
    GTATATAAAA CTGTTGTCCA TGCTCTCTCA AAACCTAAGG CCAGTGTGTC CCTGCAGAAG
    CAACAAAAGA TGGCTACCGA GGCTCCACTG AAGGACCTGT ACGGTCATGT TATGGGCTAT
    TTTGGAAGGA AGGCAGCAGC TACTAAAGAA GATTTAGCTC CCCAAGTCAA TGATGTTGCT
    GGTGCTGAAA TTGCCACTAA TGCCAATATC CCAGACCTGA TGGATGAGTT TATAGCTGAG
    AGACTGCGAT CAGGCACCAG TGTGACTATC ACACGTAGGG GCAGCAGTTC TGGTAGTCTT
    GAGGTTCCCA AAGATTTACC TGATATGCTT AATAAGCATA GCCAGATGCG CCCAGCTGAT
    GACCCTGGTG TCCCTTCAGA GTGGACCTCT CCGGCAAGCG CAGCCAGCAG TGACCTTCTT
    AGCTCAGACA GCCATTCGGA CTCTTTTAAT GCCTTCCAGT ATGATGCAAG GAAGTTTGAA
    GGTTTTCCAT TTGGAGGAGA TTCAACAGTC CCCATCTGTA TTGAAGCAGA TGCTGGCTCT
    ACACATCTTA GCGTTGAGGA ACCAGAAGTT GGAAGTCTTA CAGCACTGCA TATTGACTCT
    GAGACAAGCA GCTTCAATCA GCAGATTATG TGCACGGAGG CAGTGGCTGT AACAGGGTCA
    GAAAGTGTTT CCCCTGCTTT GTCTGCTCAT GGGTCGAGAT CAGAGACTCC CTCTCCTGCA
    GCTCTAAACT CAGATCAGGC AGAGCTACAC GACAAGCTTC AGCATTCGGT TTTACAAACT
    CCCGATGATA TAGAGCCTTG TGACTTTCCT TTAGAAAGCT GCAGTGTTAT GGCTGGAGGA
    ACACTTACTG GATGGCACGC GGACGTTTCC ACAGTCATGT GGAGAAGAAT GCTTGGGATT
    CTTGGGGATG TCAATAACAT TGCCAATCCT GAAATTCATG CCCAAGTTTT TGATTACTTG
    TGTGAACTCT GGCAGAACCT GGCTAAGATT AGAGATAATT TGGGAATATC CTTGGATAAT
    CAGTCTTCTC CCCGACCTCC TGTTCTTATC CCACCCCTGA GGATTCTCAC TCCCTGGCTT
    TTTAAGGCTA CGATGCTGCC TGAAAAATAC AAGCAGGGCA AACTCCATGC TTACAAACTG
    ATTTGCAAGA TAATGAAGAG AAGACAGGAC GTATCTCCAA ACAGGGACTT CTTAACGCAC
    TTCTATAACA TTATGCACTG TGGTCTTCTT CACAACGATC AGGACATTGT GAATACTATA
    ATCAAGCACT GTTCACCTCA ATTCTTTGCC CTTGGCCTTC CTGGTGCCAC ACTCCTTATT
    ATGGATTTTA TTGTAGCTGC TGGGAGAGTT GCTTCCTCGT CATCCCTTAA TGCTCCACGT
    GTGGAAGCTC AAGTTCTTCT GGGATCGCTT GTCTGTTTCC CCAACCTCTA CCAGGAATTG
    CCTGCTTTGC ACCCAAATAG CTCTGACATA GTAATGTCAC ATTTTACAGA TGTCAAGGAA
    CTCACAATTA AAACTGTACT AAATTCAGCC AGAGATGAAC CGTCTGGCCC TGCAAGATGT
    GTAGCATTGT GCAGTTTGGG AATATGGATG TGTGAGGAGC TTGTCAATCA AACACATCAT
    CCACAAATCA AAGAAGCACT CAATGTCATC TGTGTCTCCT TAAAATTTCC CAATAAAGTG
    GTGGCCCAGG TCGCCTGCAG TGTGCTAAAT GTGCTGCTCA ATTATGTTCA GCAGCTTCAG
    ATTTACCATT CAGACTACCC ACTTAAAATC ATTGAGATCC TGCTAGCGAC AGTGACATAT
    CTGCTGCCAA GTGCTGAGAC CTCATCTTAC GAGCAAGACA AGCGGTTGGT GGTCTCTCTG
    CTTCTTTCCC TTTTGGACTG GATGATGGCA TTACCCCTTG CATTGTTATT GAAACCACTA
    CGCCATGCTC GGACGGAGAG TGATGGAAGG GAGAACTCTG TGTTAAACTG TGCATATAAG
    GTTCTACATG ACTGTGTCTA TGGCTCCCAG CATTTCAGCA ATCCCAGGTA CTTTCCAATG
    AGCCTTTCAG ATCTGGCATC GGAGGATTAT GATCCCTTTA TGCAACTGGA ATGCCTGAAG
    GAACCAGAAC CTTTACATTC CCCAGATTCT GAGCGCTCCA CCAAACTGCA GCCTGTTACT
    GAGGTGAAAA TTCCAAAGCA GCAAGGTCTT ATTCCAGTAG CAGCACGGAC AGTAATCACA
    CATTTGGTAA ACCACTTGGG CCACTATCCA ATGAGTGGAG ACCCCGCAAT GTTGACTAGC
    CAGATCTGCG AGAACCATGA TAATCCATAC TGTGAAAGTT CGGATCTATC TCCTCAACTC
    TTCCACAGCC CCAATCTGCA GTTCTTTGCT CTCAATAATA CCACTCTTGT GTCCTGCCTT
    CAGATACAGT CTGAAGAGAA ACTACCTCCT GGTGAGGACC TCTCTTCTGC CAGATCAACT
    GTACGCATAA TTATGCGGGA CCTTTCTGGC AAGTACAGTT GGGATTCCTC TATATTATAC
    GGACCTCCTC TCCCATGTGA GGGACAAATG ACTCAGTGGT CTTCCCTTCT GGCAACATTT
    TCTCCTCCTT CCAATCAAGA GGTTGAACAC CCATCAACCC AGAGGGAATC TGAGGACTTT
    TCTTCTCTTT CTTCAAGAAG GCGAGGAAGG GAATCTCTGC CCCTATGGGA CAGCTTATCA
    GATGAACAAG ATGCTCTTGA CTGCCTTTTG CAGTATCTGG GAGCTACTGG TCCAGAGTGC
    CTTCAGAGGG CTGGTGTTCC CCTCAATGTG CCCTCACCTC CACCTGCTTG TTTCTCTGAG
    AAGCTGGAGA ATGAGGTTAG CAATGCAATT CTGAAACAGA GTGCAGAAGA AAAGGAATTT
    GTGGAAAAGC ATAGCAGTAA TCTTCACATG AGAGCAATGC CTCAAGATCA GCCTGTAAAC
    CAAAAACCCC AATCAGCTTT CTACTACTCC CGGCTGTTGC TTAGTATCCT TGGCATGAAC
    TCCTGGGAGA AGAGGCGAAA CTTCCACTTG CTTAAGAAGA ATGAGAAACT GTTACGTGAG
    CTGAAAAATT TGGACTCCAG GCAGTGCCGC GAAACCCACA AAATTGCAGT TTTCTACGTT
    GCAGACGGCC AGGAGGACAA GCATTCAATC CTATCTAACA CCGGTGGCAG TCAGGACTAT
    GAGGATTTTG TGGCTGGTCT TGGATGGGAG GTCAATCTTT CCAATCACTG TGGCTTCCTG
    GGAGGACTTC AGAGGAACAA GAGCACTGGC TTTAGCACTC CATATTTTGC AACCTCAATA
    GTGGAGGTCA TGTTCCATGT CTCCACAAGA ATGATTTCAG ACTCTGATGA TTGTTTAACC
    AAAAAGCTTC GCCACCTTGG GAATGATGAG GTGCACATTG TGTGGTCAGA ACACAGTCGG
    GAATATCGCC GCGGCATCAT ACCCACTGAA TTTGGGGACG TCCTACTTGT AATATACCCC
    ATGAAGAACC ACATGTTCAG CATCCAGATT ATGAAGAAGC CAGAAATCCC ATTTTTTGGG
    CCCCTCTTCG ATGGAGCGAT TGTGAATGGC AAAATCCTCC CAGGACTGGT TCGTGCAACA
    GCCATCAATG CCAGCAGAGC ACTTAAATCT CTTATCCCCC TGTACCAAAA TTTCTTTGAG
    GAACGGATGC GATACTTGGA TTCAATCACA CAGCAGCATT TGGAACCCAC CACCTTTGAA
    GACTACGCTG CCCAGGTGTT CTGCCCAGTG CCGCACAATC CCCTCCAGCT GAATGCACGG
    CCTTGCCCAA CGAAAGAGCA AGATGAGAAC GTATCGCCTC CGGGGGATGG ATCAGAACTG
    GCTTTTTCTT TGTCACCACG GAGCAGCAAA AGTCGCATGT CCATAAAGCT CCGGCGTTCC
    TCTGGCTCTG CCAACAAAAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746421.1
    CDS258..7601
    Translation

    Target ORF information:

    RefSeq Version XM_031890561.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Ral GTPase activating protein, alpha subunit 1 (catalytic) (ralgapa1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTTTCTA AAAAAACTCA CGGGGATGTA AAGAAATCCA CCCAGAAAGT GTTGGACCCT 
    AAAAAGGACG TTCTTACCCG CCTCAAACAC TTGCGGATCG TCATTGAAAA TGCAGAGTCC
    ATCGATCTCA AACAGTTTTT TGAACAGAAC TACTCCCATA TATATTATGT CTTCTTTGAA
    AATTTTGTGC TCATAGAAAC ATCTTTGAAA CAAAAAGGTC ACAAATCTCA AAGAGAAGAA
    CTAGATGCTA TTCTGTTTAT ATTTGAGAAA ATTTTACAAC TTCTTCCAGA GAGAATCCAC
    CAGCGATGGC AGTATCACAG CATCAGTCTG ATACTGAAAA AGCTGCTTCA TACTGGGAAT
    TCCTTAAAGA TCCGCCTTGA AGGACTCCGC TTATTCCTCC TGTGGCTTCA AGCACTCCAG
    AACAACTGCA GTTCAGAGCA GCTCCGCATC TTTGCTTGTC TCATTCCTGG TTTCCCGGCG
    CCACCATCTG ACCATGGGAT ATGCACTCTG GATAATCTAA TAAACCTTCC TTTAAATCTC
    CAAGAAAATC AAATTACAGT TGAGGAAATT ACACCACTTG TACCACCACA ATCGGGGGAA
    AAAGCTCAGG AAGATTCCAC TAGTTTCTAT CTTGAAGCTT TATTGAAGTA CATGGTAATA
    CAGGTCCGAA GTTTGGAGTG GCGCAACAAA GACAAAGAGG AATGTGGCTT TGCCTTTTTA
    TTCTCAAACT TTAAGCAATT TTACATGCCT TACATTTTCC CTGCCATGAG CAAGGAAAGC
    AGCCTATACA ACCCAGTACT TGAGATCCCA CAAATGAGAT CTAAGCCTCA TTATATGCTG
    ATCCGGAGAG ATGCAGAGAG CAATGAAGCT CAGTACTGCA CCAAAGAGCC CTTTCTCAAT
    GCTCGGGTGA CTGTCATCCG GTGGCTGGTG TCATTCTGGC TTGAACCAAA GCCACACACG
    GGAGTGCAGA TTCCTGGAAC AGAAGGGGAG AATGTGCCAA AGAATATACA GAGAGCTGCT
    GCCAGTCTGG CTGCCCAAGA GGAAAGTAGG AATGAGAGCA CAGAGCGCAC TGCTGAGCCA
    GAGCAATCTC ATTCAAACAC CAGCACCCTT ACAGAGAGAG AACCCAGTGT ATCCAGCCTC
    TGTAGTATTG CAGAGGAGCA TGTCACCGAC TACGAGATCA TCCGCAGGTT CTTTTCGAGC
    AAGCGCTGCC ATGTTGATTT TGTCATAGAG ATCTTCAGGC AGGCATTTTT GTTGCCTATA
    TGTGAAGCAG CTGCCATGCG CAAGGTGTTA AAGGTATATG AACAGTGGAT CAAACAAAAA
    GAGAAGCCTT TGTTCATGCA AGAGCCAGAT GATCCAGCCC CCACTAATGC TGCAGAAATA
    GCTGTAGAGC TCCAAGAAAT GTCCAACGTG CCTACTGAAA AGGGGAAGGA GAGGGATGAG
    GAAAAAAATT CATCATCTTC TGGACATATC CGAACCCCCA ATTTGAAAAA AAATCACAAC
    AAGCTCTCGG AAGCAGAAAT GTTGGACAGG AAAGTGAAAG GAGGGGCTCA AGCTGTGTAC
    CAGGTGTTTA TTGTCAATTC TGCAAATATC ATTTTGCTGG AGCCTGCTAA TGAGAATAAA
    ATAATGCTGA ATGAACATAC AGATATGTGC AAGCGAGTTC TGAACATTTA TCGATACATG
    GTTGTGCAAG TCTCCATGAA CAAGAAGACC TGGATGCAGT TGCTGCTTGT CATGCTAAGG
    GTCACAGAGT CTGTACTGAA GATCTCACCA CAGGTTTTAG GTCAGTATCA GAGCAGAAAG
    AATCAGATTC TGGCTGGACG GCTGGCGGGA CCCCTCTTTC AGACCCTGAT TGTTGCATGG
    ATAAAGTCAA GTCTGCATGT TAGTGTGTCT CGGGAATTGT GGGATGATCT TCTTGCTGTA
    ATGTCTTCAT TGACGTATTG GGAGGAGCAG GCTACAGAAT GGGCTCACAC CATGGAAACT
    TTGACCAAAG TGCTAGCCAA GAATGTCTAC CATCTCGACT TAAATGATTT GCCCTTAGAC
    AAGCTGAGCG AACAGAAACA GAAGAAACAC AAAGGCAAAG GGGGTGGCCA TGATTTCCAG
    AAGGTCTCTG TGGACAAGTC TTTCTCACGA GGCTGGAGTA GAGAGCAGCC TGGGCAGGCC
    CCAATGCGGC AGCGGAGTGC CACAACCACT GGTTCCCCTG GCACCGAGAA AGCCAGAAAT
    ATTGTGCGCC AGAAAACAAT TGATATGGAT GATGCTCCTA TGCATCCACG ACCATCACGA
    GTGAGGCACT ATTCTCAGAG TGAGGAGACG CCTACAGAAA CATTTGCAGC AGTAAATGAA
    GAAGCACCAA CTCTACCTCG CAGCAGCAGC ACCTCTGACA TCCTGGGCCC CTTCACAGCT
    GAAAGAACCA AAGGTGTGGT GTCTGGTACA GATCATCTCA CCCCCCAGGC TTTGGACCCC
    CCCTCTTCTA ACACACAGTC TCAGGAAGCA CCCATTACGC AGGTCGATGG CAGCATATAT
    GATCATCTCT GCCAGCTTGC TGGCTCAGCT GAATTAAGAG ATGGAATAAA GGAACAGGGC
    TTGTACGAGG AATTAGAGGG TGAAGGTCTG TTGCCACGTT TGAGAGCCTA TTTAAGGGAG
    CAGGAAGTTC TAGAAGTACT GAAAGACAGG ACGGGCAACT ACCTGTTTCA TCAAGACAGC
    GAGCATTCTG AACATGTAGA TTGTTATAAT AAAGGGGAAG TCAGTTCATT CATTCCTGAT
    TTATACAGTC AAGTAGTTTC TTCAGAGCAG AATGATAACT GTTTAGGTGG GGTGGTTAAT
    ATAGCCAAGA AAGCTGGTTG CATTAGGATG AGCAACAGGT CACGAGAAGT CCATGTAAGG
    ATACAGGTTG ATGAAGAAGA AAGGGAGAAT GATTATGTTG CCCAGGAGCA AGGTGAGCGA
    AAGAGGGGCC TCTATAGGCA ACAAGCAGCT GAGGGAGAGG AAACCTCTGA CTTGGCAACT
    TCAAAGTTTC ACCACTCCTC TGGCACTGAA CATGGGGCCA ACATAAAGAA TCTTTGTGAG
    TCTGTAACAC ACAAGGCTAA AGCAATGCGC CGGAAACGCA GCAGTGTTCA GGTTACCTTC
    CGTCCAGCCA CTGAGGCTGT GCTTTTCTAC AACTCTATGG AAAACAAAGA TGCCCCTTGG
    AAAGTGAGGC TGCGTAAATT AAGTGGTTTA AGCACTAACC CCTCTATAGC TGTAGAGCCA
    GGAAAATCCA CTGCCTATGC TGCACTTGGT GCAGTCAGAG TAGCATCAAG TGTTAGCCAG
    CCAGGTAATG ACCACCATGA TTTACCTTAC CCTCCCCCAC AAATCCAAAA GGAATGTGCC
    CGAATAAGGC AGAGATCCTG CAGTCAGGAG AGAGCCCCCT CTCATGCGTC ATTGGGTGGC
    GTATATAAAA CTGTTGTCCA TGCTCTCTCA AAACCTAAGG CCAGTGTGTC CCTGCAGAAG
    CAACAAAAGA TGGCTACCGA GGCTCCACTG AAGGACCTGT ACGGTCATGT TATGGGCTAT
    TTTGGAAGGA AGGCAGCAGC TACTAAAGAA GATTTAGCTC CCCAAGTCAA TGATGTTGCT
    GGTGCTGAAA TTGCCACTAA TGCCAATATC CCAGACCTGA TGGATGAGTT TATAGCTGAG
    AGACTGCGAT CAGGCACCAG TGTGACTATC ACACGTAGGG GCAGCAGTTC TGGTAGTCTT
    GAGGTTCCCA AAGATTTACC TGATATGCTT AATAAGCATA GCCAGATGCG CCCAGCTGAT
    GACCCTGGTG TCCCTTCAGA GTGGACCTCT CCGGCAAGCG CAGCCAGCAG TGACCTTCTT
    AGCTCAGACA GCCATTCGGA CTCTTTTAAT GCCTTCCAGT ATGATGCAAG GAAGTTTGAA
    GGTTTTCCAT TTGGAGGAGA TTCAACAGTC CCCATCTGTA TTGAAGCAGA TGCTGGCTCT
    ACACATCTTA GCGTTGAGGA ACCAGAAGTT GGAAGTCTTA CAGCACTGCA TATTGACTCT
    GAGACAAGCA GCTTCAATCA GCAGATTATG TGCACGGAGG CAGTGGCTGT AACAGGGTCA
    GAAAGTGTTT CCCCTGCTTT GTCTGCTCAT GGGTCGAGAT CAGAGACTCC CTCTCCTGCA
    GCTCTAAACT CAGATCAGGC AGAGCTACAC GACAAGCTTC AGCATTCGGT TTTACAAACT
    CCCGATGATA TAGAGCCTTG TGACTTTCCT TTAGAAAGCT GCAGTGTTAT GGCTGGAGGA
    ACACTTACTG GATGGCACGC GGACGTTTCC ACAGTCATGT GGAGAAGAAT GCTTGGGATT
    CTTGGGGATG TCAATAACAT TGCCAATCCT GAAATTCATG CCCAAGTTTT TGATTACTTG
    TGTGAACTCT GGCAGAACCT GGCTAAGATT AGAGATAATT TGGGAATATC CTTGGATAAT
    CAGTCTTCTC CCCGACCTCC TGTTCTTATC CCACCCCTGA GGATTCTCAC TCCCTGGCTT
    TTTAAGGCTA CGATGCTGCC TGAAAAATAC AAGCAGGGCA AACTCCATGC TTACAAACTG
    ATTTGCAAGA TAATGAAGAG AAGACAGGAC GTATCTCCAA ACAGGGACTT CTTAACGCAC
    TTCTATAACA TTATGCACTG TGGTCTTCTT CACAACGATC AGGACATTGT GAATACTATA
    ATCAAGCACT GTTCACCTCA ATTCTTTGCC CTTGGCCTTC CTGGTGCCAC ACTCCTTATT
    ATGGATTTTA TTGTAGCTGC TGGGAGAGTT GCTTCCTCGT CATCCCTTAA TGCTCCACGT
    GTGGAAGCTC AAGTTCTTCT GGGATCGCTT GTCTGTTTCC CCAACCTCTA CCAGGAATTG
    CCTGCTTTGC ACCCAAATAG CTCTGACATA GTAATGTCAC ATTTTACAGA TGTCAAGGAA
    CTCACAATTA AAACTGTACT AAATTCAGCC AGAGATGAAC CGTCTGGCCC TGCAAGATGT
    GTAGCATTGT GCAGTTTGGG AATATGGATG TGTGAGGAGC TTGTCAATCA AACACATCAT
    CCACAAATCA AAGAAGCACT CAATGTCATC TGTGTCTCCT TAAAATTTCC CAATAAAGTG
    GTGGCCCAGG TCGCCTGCAG TGTGCTAAAT GTGCTGCTCA ATTATGTTCA GCAGCTTCAG
    ATTTACCATT CAGACTACCC ACTTAAAATC ATTGAGATCC TGCTAGCGAC AGTGACATAT
    CTGCTGCCAA GTGCTGAGAC CTCATCTTAC GAGCAAGACA AGCGGTTGGT GGTCTCTCTG
    CTTCTTTCCC TTTTGGACTG GATGATGGCA TTACCCCTTG CATTGTTATT GAAACCACTA
    CGCCATGCTC GGACGGAGAG TGATGGAAGG GAGAACTCTG TGTTAAACTG TGCATATAAG
    GTTCTACATG ACTGTGTCTA TGGCTCCCAG CATTTCAGCA ATCCCAGGTA CTTTCCAATG
    AGCCTTTCAG ATCTGGCATC GGAGGATTAT GATCCCTTTA TGCAACTGGA ATGCCTGAAG
    GAACCAGAAC CTTTACATTC CCCAGATTCT GAGCGCTCCA CCAAACTGCA GCCTGTTACT
    GAGGTGAAAA TTCCAAAGCA GCAAGGTCTT ATTCCAGTAG CAGCACGGAC AGTAATCACA
    CATTTGGTAA ACCACTTGGG CCACTATCCA ATGAGTGGAG ACCCCGCAAT GTTGACTAGC
    CAGATCTGCG AGAACCATGA TAATCCATAC TGTGAAAGTT CGGATCTATC TCCTCAACTC
    TTCCACAGCC CCAATCTGCA GTTCTTTGCT CTCAATAATA CCACTCTTGT GTCCTGCCTT
    CAGATACAGT CTGAAGAGAA ACTACCTCCT GGTGAGGACC TCTCTTCTGC CAGATCAACT
    GTACGCATAA TTATGCGGGA CCTTTCTGGC AAGTACAGTT GGGATTCCTC TATATTATAC
    GGACCTCCTC TCCCATGTGA GGGACAAATG ACTCAGTGGT CTTCCCTTCT GGCAACATTT
    TCTCCTCCTT CCAATCAAGA GGTTGAACAC CCATCAACCC AGAGGGAATC TGAGGACTTT
    TCTTCTCTTT CTTCAAGAAG GCGAGGAAGG GAATCTCTGC CCCTATGGGA CAGCTTATCA
    GATGAACAAG ATGCTCTTGA CTGCCTTTTG CAGTATCTGG GAGCTACTGG TCCAGAGTGC
    CTTCAGAGGG CTGGTGTTCC CCTCAATGTG CCCTCACCTC CACCTGCTTG TTTCTCTGAG
    AAGCTGGAGA ATGAGGTTAG CAATGCAATT CTGAAACAGA GTGCAGAAGA AAAGGAATTT
    GTGGAAAAGC ATAGCAGTAA TCTTCACATG AGAGCAATGC CTCAAGATCA GCCTGTAAAC
    CAAAAACCCC AATCAGCTTT CTACTACTCC CGGCTGTTGC TTAGTATCCT TGGCATGAAC
    TCCTGGGAGA AGAGGCGAAA CTTCCACTTG CTTAAGAAGA ATGAGAAACT GTTACGTGAG
    CTGAAAAATT TGGACTCCAG GCAGTGCCGC GAAACCCACA AAATTGCAGT TTTCTACGTT
    GCAGACGGCC AGGAGGACAA GCATTCAATC CTATCTAACA CCGGTGGCAG TCAGGACTAT
    GAGGATTTTG TGGCTGGTCT TGGATGGGAG GTCAATCTTT CCAATCACTG TGGCTTCCTG
    GGAGGACTTC AGAGGAACAA GAGCACTGGC TTTAGCACTC CATATTTTGC AACCTCAATA
    GTGGAGGTCA TGTTCCATGT CTCCACAAGA ATGATTTCAG ACTCTGATGA TTGTTTAACC
    AAAAAGCTTC GCCACCTTGG GAATGATGAG GTGCACATTG TGTGGTCAGA ACACAGTCGG
    GAATATCGCC GCGGCATCAT ACCCACTGAA TTTGGGGACG TCCTACTTGT AATATACCCC
    ATGAAGAACC ACATGTTCAG CATCCAGATT ATGAAGAAGC CAGAAATCCC ATTTTTTGGG
    CCCCTCTTCG ATGGAGCGAT TGTGAATGGC AAAATCCTCC CAGGACTGGT TCGTGCAACA
    GCCATCAATG CCAGCAGAGC ACTTAAATCT CTTATCCCCC TGTACCAAAA TTTCTTTGAG
    GAACGGATGC GATACTTGGA TTCAATCACA CAGCAGCATT TGGAACCCAC CACCTTTGAA
    GACTACGCTG CCCAGGTGTT CTGCCCAGTG CCGCACAATC CCCTCCAGCT GAATGCACGG
    CCTTGCCCAA CGAAAGAGCA AGATGAGAAC GTATCGCCTC CGGGGGATGG ATCAGAACTG
    GCTTTTTCTT TGTCACCACG GAGCAGCAAA AGTCGCATGT CCATAAAGCT CCGGCGTTCC
    TCTGGCTCTG CCAACAAAAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.