plxnb2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	plxnb2
Entrez Gene ID	550076
Full Name	plexin B2
General protein information	Preferred Names plexin B2 Names plexin-B2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012959116.2	XP_012814570.1	plexin-B2 isoform X1
XM_012959119.2	XP_012814573.1	plexin-B2 isoform X1
NM_001017322.2	NP_001017322.1	plexin-B2
XM_018092115.1	XP_017947604.1	plexin-B2 isoform X1
NM_001017322.3	NP_001017322.2	plexin-B2 precursor
XM_018092116.1	XP_017947605.1	plexin-B2 isoform X1
XM_012959119.3	XP_012814573.2	plexin-B2 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following plxnb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plxnb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25191	NM_001017322.2 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OXa28190	NM_001017322.3 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53578	XM_012959119.3 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa28190	XM_012959116.2 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa28190	XM_018092116.1 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa28190	XM_012959119.2 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa28190	XM_018092115.1 Latest version!	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plxnb2 ( NM_001017322.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001017322.1 Xenopus tropicalis plexin B2 (plxnb2), mRNA.

Price & Availability↑

CloneID	OXa25191
Clone ID Related Accession (Same CDS sequence)	NM_001017322.2
Accession Version	NM_001017322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR848639.2. On Oct 14, 2005 this sequence version replaced NM_001017322.1. ##Evidence-Data-START## Transcript exon combination :: CR848639.2, CX881887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGAAA AGGAAAGAAC CAAAGCAATA ACAGAAATAT ATCTAACTAG GCTGCTTTCT 
GTCAAGGGTA CACTCCAGCA GTTTGTAGAT AACTTCTTCC AGAGTGTCCT TAATTCCAAT 
CAAGTGGTAC CACCAGCTGT GAAATACTTC TTCGATTTCC TTGATGAGCA AGCAGAAAAA 
TATGAAATCA AAGATGAAGA TACTGTCCAC ATATGGAAAA CAAACAGTTT ATCACTAAGA 
TTTTGGGTGA ATATACTGAA AAATCCACAC TTTATTTTTG ATGTGCATGT TCACCAAGTA 
GTAGATGCCT CCTTGTCAGT CATCGCACAG ACTTTCATGG ATGCCTGCAG CAGGACGGAG 
CACAAGCTTA GCAGAGAATC TCCAAGCAAC AAGCTCTTGT ATGCAAAAGA GATCTCAACT 
TACAAAAAAA TGGTGGAAGA CTATTACAAG GGAATCCGCC AGATGGTACA AGTTAGCGAC 
CAGGATATGA ATACACATTT AGCAGAAATT TCAAGGGCTC ACACGGAGTC ATTAAATACA 
CTAGTAGCAC TACATCAGCT GTATCAGTAT ACAAATAAAT ACTACGATGA GATAATAAAC 
GCTCTGGAGG AGGATCCTGC AGCCCAGCGC ATGCAGTTAG CATATCGGCT ACAGCAAATA 
GCCGCAGCAC TAGAGAACAA AGTGACAGAT CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017322.1
CDS	30..725
Translation	MKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	NM_001017322.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017322.2

ORF Insert Sequence:

ATGAAAGAAA AGGAAAGAAC CAAAGCAATA ACAGAAATAT ATCTAACTAG GCTGCTTTCT 
GTCAAGGGTA CACTCCAGCA GTTTGTAGAT AACTTCTTCC AGAGTGTCCT TAATTCCAAT 
CAAGTGGTAC CACCAGCTGT GAAATACTTC TTCGATTTCC TTGATGAGCA AGCAGAAAAA 
TATGAAATCA AAGATGAAGA TACTGTCCAC ATATGGAAAA CAAACAGTTT ATCACTAAGA 
TTTTGGGTGA ATATACTGAA AAATCCACAC TTTATTTTTG ATGTGCATGT TCACCAAGTA 
GTAGATGCCT CCTTGTCAGT CATCGCACAG ACTTTCATGG ATGCCTGCAG CAGGACGGAG 
CACAAGCTTA GCAGAGAATC TCCAAGCAAC AAGCTCTTGT ATGCAAAAGA GATCTCAACT 
TACAAAAAAA TGGTGGAAGA CTATTACAAG GGAATCCGCC AGATGGTACA AGTTAGCGAC 
CAGGATATGA ATACACATTT AGCAGAAATT TCAAGGGCTC ACACGGAGTC ATTAAATACA 
CTAGTAGCAC TACATCAGCT GTATCAGTAT ACAAATAAAT ACTACGATGA GATAATAAAC 
GCTCTGGAGG AGGATCCTGC AGCCCAGCGC ATGCAGTTAG CATATCGGCT ACAGCAAATA 
GCCGCAGCAC TAGAGAACAA AGTGACAGAT CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plxnb2 ( NM_001017322.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001017322.2 Xenopus tropicalis plexin B2 (plxnb2), mRNA.

Price & Availability↑

CloneID	OXa28190
Clone ID Related Accession (Same CDS sequence)	XM_012959116.2 , XM_012959119.2 , XM_018092115.1 , NM_001017322.3 , XM_018092116.1
Accession Version	NM_001017322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-08
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03011622.1 and AAMC03011623.1. On or before Oct 9, 2019 this sequence version replaced XM_012959116.2, NM_001017322.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017322.2
CDS	458..5956
Translation	MALWFQVSSLIICIILMPQAQTFLSFFSDTDLNHLAVNNDTGIVYIGAVNRLYQLSAELDMQVNFTTGPKLDKKSCTPPIDSQQCPSAEITDNYNKLLLLDKPHERIIVCGSIFKGICSLRAMSDISTELWYKDNLGEKSFVASNDEVISTVGLVTNHGKEKRRLLFVGKGHVQNDNGIIISTRILEKKEDRDVFEVYQDSATFKSVDHIKHFQQFVTAFENDDYVYFVSTRADRISNENRTFISRLCKDDFNYYSYIEMPLECKNQRNNESYNFSHSVFVTKPGSDLADIMKLPSTETTVLFGVFSRKQVSDQSAVCVFSLSEINNKFEENREACYTSNKADLKTLFFKPYGGELSCANSPSGAAKSYRCGSEHLPYPLECKEGVSKSSVLIREKNPFSSVAIAVENEHTIAFLGTAEGKLVKAFIRDQSYEYRNLSFETSSAVKNGMVFDADQKHLYVMTKKKAYRVPVQDCARSSNCNECISTNDPYCGWCVLEGKCTRKVDCPRNNNSHWLFSPSGTCMQVKSATPGNMSVMSLRKVTLTVEPSIYLLYNDNLICDFGGAKTTAELQQDGTISCQPPNLIPSPEKGKDNVNITINLSFQKNGDVFFANYSYPFYNCKEAINLSENMPCTSCVTSRWNCQWDINNHRCEDSSQRDDVIEPNMEENCPQFYEPNPSLIPMKHKSTITFNGKNLDHYKNEDFSAGSDEFTFKTDVLQIDGSFSLSIPEFPAEDKETVPLGIYIKVNEKKIDSKLNVNLYNCMYGRSDCSLCLAADPVYQCVWCNDKCVYKDMCEESPATECPAPKITDLVPIAAPLKGGIRLTIKGSNLGIKPADVEEIIVANTPCTFIEELYSVSTSVVCEVGAVDEPDSGNVKIKIKEKMGISSQIFTYQDPNPDYLHPLEGIMAGGTRLTIKGQRLETGSENDIRVTLDSIPCAVKSFGEEIVCDTGMASKKGEVAVKLLYGDRTEINAGHFIYKENPSIAHFTPDRSFASGGRHITISGSGFNLVQNFRIIARLQGEDSSSVTEDESKTISQEDILEKVNDSVIIFTSPQVPENYLTSGVTIVIKMDGLESPLQTNELSFLYIPDPTFENFTDGIKKQVNNLINAKGDNLNAAMTIEEAKAFVGEGTCKINTLTSTDLYCVPPEEQPPPKRRHKRDTANNLPEFIVKFGKREWVLGKVEYGQSPAIPLHIIIPVVIIPMLLIIGIAVYFYRRKSQQAEREYEKVKLQLEGLEETVRERCKREFTELMIEMEDQTSDLSEAGIPFLDYKTYTDRVFFLSSKDGEKDVMITGKLDIPEARRQTVEVALNQFSNLLNSKSFLTIFIHTLESQKDFSAREKLYLASLLTVALHGKLEYYTDIMRALLMELMDQYVAKNPKLLLRRSETVVERMLSNWMSICLYQYLKDTVGEPLYKLFKAIKHQVEKGPVDALQAKAKYTLNDTGLLGDDVEYKQLTVNVIVQEEGAEPVPVKVLNCDTITQVKEKIVDQVYRNIPYSLRPKADSLALEWRPGSTAQILSDLDLTSQKEGRLKRYNTLMHYNVRDNATLILSRMGISQQQEENHQDFPGERNALLEDENKAWHLVRPADEVDEVKSKRGSMKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	NM_001017322.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017322.3

ORF Insert Sequence:

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plxnb2 ( XM_012959119.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814573.2 Xenopus tropicalis plexin B2 (plxnb2), transcript variant X1, mRNA.

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CloneID	OXa53578
Clone ID Related Accession (Same CDS sequence)	XM_012959119.3
Accession Version	XM_012959119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAT CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTA 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACACGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCTGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GGAGATTTCA GTGCAGGCAA TGATGAATTT AAGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA AAGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTCTCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CTGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACGTATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTG 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CTTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814573.2
CDS	380..5878
Translation	MALWFQVSSLIICIILMPQAQTFLSFFSDTDLNHLAVNNDTGIVYIGAVNRLYQLSAELDMQVNFTTGPKLDKKSCTPPIDSQQCPSAEITDNYNKLLLLDKPHERIIVCGSIFKGICSLRAMSDISTELWYKDNLGEKSFVASNDEVISTVGLVTNHGKEKRRLLFVGKGHVQNDNGIIISTRILEKKEDRDVFEVYQDSATFKSVDHIKHFQQFVTAFENDDYVYFVSTRADRISNENRTFISRLCKDDFNYYSYIEMPLECKNQRNNESYNFSHSVFVTKPGSDLADIMKLPSTETTVLFGVFSRKQVSDQSAVCVFSLSEINNKFEENREACYTSNKADLKTLFFKPYGGELSCANSPSGAAKSYRCGSEHLPYPLECKEGVSKSSVLIREKNPFSSVAIAVENEHTIAFLGTAEGKLVKAFIRDQSYEYRNLSFETSSAVKNGMVFDADQKHLYVMTKKKAYRVPVQDCARSSNCNECISTNDPYCGWCVLEGKCTRKVDCPRNNNSHWLFSPSGTCMQVKSATPGNMSVMSLRKVTLTVEPSIYLLYNDNLICDFGGAKTTAELQQDGTISCQPPNLIPSPEKGKDNVNITINLSFQKNGDVFFANYSYPFYNCKEAINLSENMPCTSCVTSRWNCQWDINNHRCEDSSQRDDVIEPNMEENCPQFYEPNPSLIPMKHKSTITFNGKNLDHYKNGDFSAGNDEFKFKTDVLQIDGSFSLSIPEFPAEDKETVPLGIYIKVNEKKIDSKLNVNLYNCMYGRSDCSLCLAADPVYQCVWCNDKCVYKDMCEESPATECPAPKITDLVPIAAPLKGGIRLTIKGSNLGIKPADVEEIIVANTPCTFIEELYSVSTSVVCEVGAVDEPDSGKVKIKIKEKMGISSQIFTYQDPNPDSLHPLEGIMAGGTRLTIKGQRLETGSENDIRVTLDSIPCAVKSFGEEIVCDTGMASKKGEVAVKLLYGDCTEINAGHFIYKENPSIAHFTPDRSFASGGRRITISGSGFNLVQNFRIIARLQGEDSSSVTEDESKTISQEDILEKVNDSVIIFTSPQVPENYLTSGVTIVIKMDGLESPLQTNELSFLYIPDPTFENFTDGIKKQVNNLINAKGDNLNAAMTIEEAKAFVGEGTCKINTLTSTDLYCVPPEEQPPPKRRHKRDTANNLPEFIVKFGKREWVLGKVEYGQSPAIPLHIIIPVVIIPMLLIIGIAVYFYRRKSQQAEREYEKVKLQLEGLEETVRERCKREFTELMIEMEDQTSDLSEAGIPFLDYKTYTDRVFFLSSKDGEKDVMITGKLDIPEARRQTVEVALNQFSNLLNSKSFLTIFIHTLESQKDFSAREKLYLASLLTVALHGKLEYYTDIMRALLMELMDQYVAKNPKLLLRRSETVVERMLSNWMSICLYQYLKDTVGEPLYKLFKAIKHQVEKGPVDALQAKAKYTLNDTGLLGDDVEYKQLTVNVIVQEEGAEPVPVKVLNCDTITQVKEKIVDQVYRNIPYSLRPKADSLALEWRPGSTAQILSDLDLTSQKEGRLKRYNTLMHYNVRDNATLILSRMGISQQQEENHQDFPGERNALLEDENKAWHLVRPADEVDEVKSKRGSMKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_012959119.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959119.3

ORF Insert Sequence:

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAT CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTA 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACACGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCTGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GGAGATTTCA GTGCAGGCAA TGATGAATTT AAGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA AAGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTCTCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CTGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACGTATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTG 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CTTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plxnb2 ( XM_012959116.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814570.1 Xenopus tropicalis plexin B2 (plxnb2), transcript variant X1, mRNA.

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CloneID	OXa28190
Clone ID Related Accession (Same CDS sequence)	XM_012959116.2 , XM_012959119.2 , XM_018092115.1 , NM_001017322.3 , XM_018092116.1
Accession Version	XM_012959116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814570.1
CDS	458..5956
Translation	MALWFQVSSLIICIILMPQAQTFLSFFSDTDLNHLAVNNDTGIVYIGAVNRLYQLSAELDMQVNFTTGPKLDKKSCTPPIDSQQCPSAEITDNYNKLLLLDKPHERIIVCGSIFKGICSLRAMSDISTELWYKDNLGEKSFVASNDEVISTVGLVTNHGKEKRRLLFVGKGHVQNDNGIIISTRILEKKEDRDVFEVYQDSATFKSVDHIKHFQQFVTAFENDDYVYFVSTRADRISNENRTFISRLCKDDFNYYSYIEMPLECKNQRNNESYNFSHSVFVTKPGSDLADIMKLPSTETTVLFGVFSRKQVSDQSAVCVFSLSEINNKFEENREACYTSNKADLKTLFFKPYGGELSCANSPSGAAKSYRCGSEHLPYPLECKEGVSKSSVLIREKNPFSSVAIAVENEHTIAFLGTAEGKLVKAFIRDQSYEYRNLSFETSSAVKNGMVFDADQKHLYVMTKKKAYRVPVQDCARSSNCNECISTNDPYCGWCVLEGKCTRKVDCPRNNNSHWLFSPSGTCMQVKSATPGNMSVMSLRKVTLTVEPSIYLLYNDNLICDFGGAKTTAELQQDGTISCQPPNLIPSPEKGKDNVNITINLSFQKNGDVFFANYSYPFYNCKEAINLSENMPCTSCVTSRWNCQWDINNHRCEDSSQRDDVIEPNMEENCPQFYEPNPSLIPMKHKSTITFNGKNLDHYKNEDFSAGSDEFTFKTDVLQIDGSFSLSIPEFPAEDKETVPLGIYIKVNEKKIDSKLNVNLYNCMYGRSDCSLCLAADPVYQCVWCNDKCVYKDMCEESPATECPAPKITDLVPIAAPLKGGIRLTIKGSNLGIKPADVEEIIVANTPCTFIEELYSVSTSVVCEVGAVDEPDSGNVKIKIKEKMGISSQIFTYQDPNPDYLHPLEGIMAGGTRLTIKGQRLETGSENDIRVTLDSIPCAVKSFGEEIVCDTGMASKKGEVAVKLLYGDRTEINAGHFIYKENPSIAHFTPDRSFASGGRHITISGSGFNLVQNFRIIARLQGEDSSSVTEDESKTISQEDILEKVNDSVIIFTSPQVPENYLTSGVTIVIKMDGLESPLQTNELSFLYIPDPTFENFTDGIKKQVNNLINAKGDNLNAAMTIEEAKAFVGEGTCKINTLTSTDLYCVPPEEQPPPKRRHKRDTANNLPEFIVKFGKREWVLGKVEYGQSPAIPLHIIIPVVIIPMLLIIGIAVYFYRRKSQQAEREYEKVKLQLEGLEETVRERCKREFTELMIEMEDQTSDLSEAGIPFLDYKTYTDRVFFLSSKDGEKDVMITGKLDIPEARRQTVEVALNQFSNLLNSKSFLTIFIHTLESQKDFSAREKLYLASLLTVALHGKLEYYTDIMRALLMELMDQYVAKNPKLLLRRSETVVERMLSNWMSICLYQYLKDTVGEPLYKLFKAIKHQVEKGPVDALQAKAKYTLNDTGLLGDDVEYKQLTVNVIVQEEGAEPVPVKVLNCDTITQVKEKIVDQVYRNIPYSLRPKADSLALEWRPGSTAQILSDLDLTSQKEGRLKRYNTLMHYNVRDNATLILSRMGISQQQEENHQDFPGERNALLEDENKAWHLVRPADEVDEVKSKRGSMKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_012959116.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959116.2

ORF Insert Sequence:

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plxnb2 ( XM_012959119.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814573.1 Xenopus tropicalis plexin B2 (plxnb2), transcript variant X3, mRNA.

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CloneID	OXa28190
Clone ID Related Accession (Same CDS sequence)	XM_012959116.2 , XM_012959119.2 , XM_018092115.1 , NM_001017322.3 , XM_018092116.1
Accession Version	XM_012959119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814573.1
CDS	395..5893
Translation	MALWFQVSSLIICIILMPQAQTFLSFFSDTDLNHLAVNNDTGIVYIGAVNRLYQLSAELDMQVNFTTGPKLDKKSCTPPIDSQQCPSAEITDNYNKLLLLDKPHERIIVCGSIFKGICSLRAMSDISTELWYKDNLGEKSFVASNDEVISTVGLVTNHGKEKRRLLFVGKGHVQNDNGIIISTRILEKKEDRDVFEVYQDSATFKSVDHIKHFQQFVTAFENDDYVYFVSTRADRISNENRTFISRLCKDDFNYYSYIEMPLECKNQRNNESYNFSHSVFVTKPGSDLADIMKLPSTETTVLFGVFSRKQVSDQSAVCVFSLSEINNKFEENREACYTSNKADLKTLFFKPYGGELSCANSPSGAAKSYRCGSEHLPYPLECKEGVSKSSVLIREKNPFSSVAIAVENEHTIAFLGTAEGKLVKAFIRDQSYEYRNLSFETSSAVKNGMVFDADQKHLYVMTKKKAYRVPVQDCARSSNCNECISTNDPYCGWCVLEGKCTRKVDCPRNNNSHWLFSPSGTCMQVKSATPGNMSVMSLRKVTLTVEPSIYLLYNDNLICDFGGAKTTAELQQDGTISCQPPNLIPSPEKGKDNVNITINLSFQKNGDVFFANYSYPFYNCKEAINLSENMPCTSCVTSRWNCQWDINNHRCEDSSQRDDVIEPNMEENCPQFYEPNPSLIPMKHKSTITFNGKNLDHYKNEDFSAGSDEFTFKTDVLQIDGSFSLSIPEFPAEDKETVPLGIYIKVNEKKIDSKLNVNLYNCMYGRSDCSLCLAADPVYQCVWCNDKCVYKDMCEESPATECPAPKITDLVPIAAPLKGGIRLTIKGSNLGIKPADVEEIIVANTPCTFIEELYSVSTSVVCEVGAVDEPDSGNVKIKIKEKMGISSQIFTYQDPNPDYLHPLEGIMAGGTRLTIKGQRLETGSENDIRVTLDSIPCAVKSFGEEIVCDTGMASKKGEVAVKLLYGDRTEINAGHFIYKENPSIAHFTPDRSFASGGRHITISGSGFNLVQNFRIIARLQGEDSSSVTEDESKTISQEDILEKVNDSVIIFTSPQVPENYLTSGVTIVIKMDGLESPLQTNELSFLYIPDPTFENFTDGIKKQVNNLINAKGDNLNAAMTIEEAKAFVGEGTCKINTLTSTDLYCVPPEEQPPPKRRHKRDTANNLPEFIVKFGKREWVLGKVEYGQSPAIPLHIIIPVVIIPMLLIIGIAVYFYRRKSQQAEREYEKVKLQLEGLEETVRERCKREFTELMIEMEDQTSDLSEAGIPFLDYKTYTDRVFFLSSKDGEKDVMITGKLDIPEARRQTVEVALNQFSNLLNSKSFLTIFIHTLESQKDFSAREKLYLASLLTVALHGKLEYYTDIMRALLMELMDQYVAKNPKLLLRRSETVVERMLSNWMSICLYQYLKDTVGEPLYKLFKAIKHQVEKGPVDALQAKAKYTLNDTGLLGDDVEYKQLTVNVIVQEEGAEPVPVKVLNCDTITQVKEKIVDQVYRNIPYSLRPKADSLALEWRPGSTAQILSDLDLTSQKEGRLKRYNTLMHYNVRDNATLILSRMGISQQQEENHQDFPGERNALLEDENKAWHLVRPADEVDEVKSKRGSMKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_012959119.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959119.2

ORF Insert Sequence:

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plxnb2 ( XM_018092115.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947604.1 Xenopus tropicalis plexin B2 (plxnb2), transcript variant X2, mRNA.

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CloneID	OXa28190
Clone ID Related Accession (Same CDS sequence)	XM_012959116.2 , XM_012959119.2 , XM_018092115.1 , NM_001017322.3 , XM_018092116.1
Accession Version	XM_018092115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947604.1
CDS	570..6068
Translation	MALWFQVSSLIICIILMPQAQTFLSFFSDTDLNHLAVNNDTGIVYIGAVNRLYQLSAELDMQVNFTTGPKLDKKSCTPPIDSQQCPSAEITDNYNKLLLLDKPHERIIVCGSIFKGICSLRAMSDISTELWYKDNLGEKSFVASNDEVISTVGLVTNHGKEKRRLLFVGKGHVQNDNGIIISTRILEKKEDRDVFEVYQDSATFKSVDHIKHFQQFVTAFENDDYVYFVSTRADRISNENRTFISRLCKDDFNYYSYIEMPLECKNQRNNESYNFSHSVFVTKPGSDLADIMKLPSTETTVLFGVFSRKQVSDQSAVCVFSLSEINNKFEENREACYTSNKADLKTLFFKPYGGELSCANSPSGAAKSYRCGSEHLPYPLECKEGVSKSSVLIREKNPFSSVAIAVENEHTIAFLGTAEGKLVKAFIRDQSYEYRNLSFETSSAVKNGMVFDADQKHLYVMTKKKAYRVPVQDCARSSNCNECISTNDPYCGWCVLEGKCTRKVDCPRNNNSHWLFSPSGTCMQVKSATPGNMSVMSLRKVTLTVEPSIYLLYNDNLICDFGGAKTTAELQQDGTISCQPPNLIPSPEKGKDNVNITINLSFQKNGDVFFANYSYPFYNCKEAINLSENMPCTSCVTSRWNCQWDINNHRCEDSSQRDDVIEPNMEENCPQFYEPNPSLIPMKHKSTITFNGKNLDHYKNEDFSAGSDEFTFKTDVLQIDGSFSLSIPEFPAEDKETVPLGIYIKVNEKKIDSKLNVNLYNCMYGRSDCSLCLAADPVYQCVWCNDKCVYKDMCEESPATECPAPKITDLVPIAAPLKGGIRLTIKGSNLGIKPADVEEIIVANTPCTFIEELYSVSTSVVCEVGAVDEPDSGNVKIKIKEKMGISSQIFTYQDPNPDYLHPLEGIMAGGTRLTIKGQRLETGSENDIRVTLDSIPCAVKSFGEEIVCDTGMASKKGEVAVKLLYGDRTEINAGHFIYKENPSIAHFTPDRSFASGGRHITISGSGFNLVQNFRIIARLQGEDSSSVTEDESKTISQEDILEKVNDSVIIFTSPQVPENYLTSGVTIVIKMDGLESPLQTNELSFLYIPDPTFENFTDGIKKQVNNLINAKGDNLNAAMTIEEAKAFVGEGTCKINTLTSTDLYCVPPEEQPPPKRRHKRDTANNLPEFIVKFGKREWVLGKVEYGQSPAIPLHIIIPVVIIPMLLIIGIAVYFYRRKSQQAEREYEKVKLQLEGLEETVRERCKREFTELMIEMEDQTSDLSEAGIPFLDYKTYTDRVFFLSSKDGEKDVMITGKLDIPEARRQTVEVALNQFSNLLNSKSFLTIFIHTLESQKDFSAREKLYLASLLTVALHGKLEYYTDIMRALLMELMDQYVAKNPKLLLRRSETVVERMLSNWMSICLYQYLKDTVGEPLYKLFKAIKHQVEKGPVDALQAKAKYTLNDTGLLGDDVEYKQLTVNVIVQEEGAEPVPVKVLNCDTITQVKEKIVDQVYRNIPYSLRPKADSLALEWRPGSTAQILSDLDLTSQKEGRLKRYNTLMHYNVRDNATLILSRMGISQQQEENHQDFPGERNALLEDENKAWHLVRPADEVDEVKSKRGSMKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_018092115.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092115.1

ORF Insert Sequence:

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plxnb2 ( XM_018092116.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947605.1 Xenopus tropicalis plexin B2 (plxnb2), transcript variant X4, mRNA.

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CloneID	OXa28190
Clone ID Related Accession (Same CDS sequence)	XM_012959116.2 , XM_012959119.2 , XM_018092115.1 , NM_001017322.3 , XM_018092116.1
Accession Version	XM_018092116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	plexin-B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947605.1
CDS	133..5631
Translation	MALWFQVSSLIICIILMPQAQTFLSFFSDTDLNHLAVNNDTGIVYIGAVNRLYQLSAELDMQVNFTTGPKLDKKSCTPPIDSQQCPSAEITDNYNKLLLLDKPHERIIVCGSIFKGICSLRAMSDISTELWYKDNLGEKSFVASNDEVISTVGLVTNHGKEKRRLLFVGKGHVQNDNGIIISTRILEKKEDRDVFEVYQDSATFKSVDHIKHFQQFVTAFENDDYVYFVSTRADRISNENRTFISRLCKDDFNYYSYIEMPLECKNQRNNESYNFSHSVFVTKPGSDLADIMKLPSTETTVLFGVFSRKQVSDQSAVCVFSLSEINNKFEENREACYTSNKADLKTLFFKPYGGELSCANSPSGAAKSYRCGSEHLPYPLECKEGVSKSSVLIREKNPFSSVAIAVENEHTIAFLGTAEGKLVKAFIRDQSYEYRNLSFETSSAVKNGMVFDADQKHLYVMTKKKAYRVPVQDCARSSNCNECISTNDPYCGWCVLEGKCTRKVDCPRNNNSHWLFSPSGTCMQVKSATPGNMSVMSLRKVTLTVEPSIYLLYNDNLICDFGGAKTTAELQQDGTISCQPPNLIPSPEKGKDNVNITINLSFQKNGDVFFANYSYPFYNCKEAINLSENMPCTSCVTSRWNCQWDINNHRCEDSSQRDDVIEPNMEENCPQFYEPNPSLIPMKHKSTITFNGKNLDHYKNEDFSAGSDEFTFKTDVLQIDGSFSLSIPEFPAEDKETVPLGIYIKVNEKKIDSKLNVNLYNCMYGRSDCSLCLAADPVYQCVWCNDKCVYKDMCEESPATECPAPKITDLVPIAAPLKGGIRLTIKGSNLGIKPADVEEIIVANTPCTFIEELYSVSTSVVCEVGAVDEPDSGNVKIKIKEKMGISSQIFTYQDPNPDYLHPLEGIMAGGTRLTIKGQRLETGSENDIRVTLDSIPCAVKSFGEEIVCDTGMASKKGEVAVKLLYGDRTEINAGHFIYKENPSIAHFTPDRSFASGGRHITISGSGFNLVQNFRIIARLQGEDSSSVTEDESKTISQEDILEKVNDSVIIFTSPQVPENYLTSGVTIVIKMDGLESPLQTNELSFLYIPDPTFENFTDGIKKQVNNLINAKGDNLNAAMTIEEAKAFVGEGTCKINTLTSTDLYCVPPEEQPPPKRRHKRDTANNLPEFIVKFGKREWVLGKVEYGQSPAIPLHIIIPVVIIPMLLIIGIAVYFYRRKSQQAEREYEKVKLQLEGLEETVRERCKREFTELMIEMEDQTSDLSEAGIPFLDYKTYTDRVFFLSSKDGEKDVMITGKLDIPEARRQTVEVALNQFSNLLNSKSFLTIFIHTLESQKDFSAREKLYLASLLTVALHGKLEYYTDIMRALLMELMDQYVAKNPKLLLRRSETVVERMLSNWMSICLYQYLKDTVGEPLYKLFKAIKHQVEKGPVDALQAKAKYTLNDTGLLGDDVEYKQLTVNVIVQEEGAEPVPVKVLNCDTITQVKEKIVDQVYRNIPYSLRPKADSLALEWRPGSTAQILSDLDLTSQKEGRLKRYNTLMHYNVRDNATLILSRMGISQQQEENHQDFPGERNALLEDENKAWHLVRPADEVDEVKSKRGSMKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLNSNQVVPPAVKYFFDFLDEQAEKYEIKDEDTVHIWKTNSLSLRFWVNILKNPHFIFDVHVHQVVDASLSVIAQTFMDACSRTEHKLSRESPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTESLNTLVALHQLYQYTNKYYDEIINALEEDPAAQRMQLAYRLQQIAAALENKVTDL

Target ORF information:

RefSeq Version	XM_018092116.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis plexin B2 (plxnb2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092116.1

ORF Insert Sequence:

ATGGCTTTAT GGTTTCAAGT TTCCAGCCTA ATTATCTGCA TCATATTGAT GCCACAAGCA 
CAAACATTTC TCTCTTTCTT TAGTGACACA GATTTAAACC ATCTTGCTGT TAACAATGAC 
ACTGGCATTG TTTATATTGG AGCTGTAAAC CGCTTGTACC AACTTTCTGC AGAGCTCGAT 
ATGCAAGTGA ATTTTACCAC AGGTCCAAAA TTAGATAAAA AATCTTGCAC ACCACCCATT 
GACTCCCAGC AGTGTCCCTC TGCAGAAATA ACAGATAATT ATAACAAGCT GCTGCTGCTA 
GATAAGCCAC ATGAACGGAT TATTGTATGT GGTAGCATTT TTAAAGGAAT TTGCTCGTTG 
AGGGCTATGA GCGATATTTC TACAGAGCTT TGGTATAAAG ATAACCTAGG AGAAAAGTCT 
TTTGTTGCGA GCAATGATGA AGTTATATCT ACTGTTGGAT TGGTCACTAA CCATGGCAAA 
GAAAAAAGGA GGCTTTTATT TGTTGGAAAA GGCCATGTAC AAAATGACAA TGGAATTATC 
ATAAGTACGC GGATACTGGA AAAGAAAGAA GATAGAGATG TTTTTGAAGT GTATCAAGAC 
TCTGCAACAT TTAAATCTGT TGATCATATA AAACATTTCC AGCAATTTGT AACTGCTTTT 
GAGAATGATG ATTATGTTTA TTTTGTCTCA ACGCGAGCAG ATCGAATTAG TAATGAAAAT 
CGGACTTTTA TTTCCCGTCT TTGTAAGGAT GATTTCAACT ACTACTCCTA TATTGAGATG 
CCTCTTGAAT GTAAAAACCA GAGGAATAAT GAGAGCTACA ATTTCAGCCA CTCTGTTTTT 
GTTACTAAAC CGGGCAGTGA CTTAGCAGAT ATCATGAAAC TTCCATCCAC TGAAACAACA 
GTTCTCTTTG GTGTTTTTAG CAGAAAACAA GTTTCAGATC AATCTGCGGT CTGTGTGTTT 
TCACTGTCTG AGATTAATAA TAAATTTGAA GAAAACCGTG AGGCATGTTA CACAAGTAAT 
AAAGCTGATC TCAAGACTCT ATTTTTTAAG CCATATGGAG GAGAACTGTC CTGTGCAAAT 
TCACCATCGG GAGCTGCTAA AAGTTATCGC TGTGGGTCAG AACACTTGCC CTACCCCTTG 
GAGTGCAAGG AAGGTGTATC CAAAAGTTCT GTGTTAATAA GAGAGAAGAA TCCTTTTAGC 
TCAGTTGCCA TCGCTGTTGA GAATGAGCAC ACCATTGCAT TTTTGGGGAC AGCTGAAGGA 
AAACTTGTGA AGGCTTTTAT CAGAGACCAA TCATATGAGT ATAGGAACCT TTCCTTTGAA 
ACATCAAGTG CAGTGAAGAA TGGCATGGTG TTTGATGCTG ATCAAAAACA TCTGTATGTA 
ATGACTAAGA AAAAGGCCTA CCGAGTTCCT GTTCAAGATT GTGCCCGTTC TTCAAATTGC 
AATGAATGCA TTTCAACAAA TGATCCTTAT TGTGGTTGGT GTGTTCTTGA AGGAAAATGC 
ACAAGGAAGG TTGATTGCCC AAGGAACAAT AACAGTCACT GGTTATTTAG TCCTTCTGGA 
ACATGTATGC AAGTCAAGAG TGCGACGCCA GGGAATATGA GTGTCATGTC ACTGCGCAAG 
GTTACATTAA CTGTGGAACC CTCAATATAT TTATTATATA ACGATAATCT GATTTGTGAC 
TTTGGAGGAG CGAAAACTAC AGCTGAATTG CAGCAAGATG GGACAATCTC TTGCCAACCC 
CCAAATCTAA TTCCATCCCC AGAAAAAGGC AAAGATAATG TTAACATTAC AATCAACCTA 
AGCTTTCAGA AGAATGGTGA TGTGTTTTTT GCAAATTATT CTTATCCATT CTATAATTGT 
AAAGAAGCAA TAAACCTTTC AGAGAATATG CCATGTACAT CCTGTGTTAC TAGCAGGTGG 
AATTGTCAGT GGGATATAAA TAATCATAGG TGTGAGGACT CCTCTCAACG GGATGATGTT 
ATTGAGCCTA ATATGGAAGA AAATTGTCCA CAGTTCTATG AACCCAATCC ATCATTGATT 
CCAATGAAAC ATAAAAGTAC CATAACATTT AATGGGAAAA ATCTTGATCA TTATAAGAAT 
GAAGATTTCA GTGCAGGCAG TGATGAATTT ACGTTCAAAA CAGATGTGTT ACAAATTGAT 
GGATCCTTCT CCCTTTCTAT ACCAGAGTTT CCTGCAGAAG ATAAAGAAAC TGTTCCTTTG 
GGTATTTACA TTAAAGTGAA TGAAAAAAAG ATTGACAGTA AATTAAATGT TAATCTCTAC 
AACTGCATGT ATGGCAGAAG TGACTGCAGC CTGTGCCTTG CTGCCGATCC TGTATACCAG 
TGTGTCTGGT GTAATGACAA ATGTGTATAC AAGGATATGT GTGAGGAATC ACCTGCTACT 
GAGTGCCCAG CTCCTAAAAT CACTGATCTT GTGCCAATAG CAGCACCTTT AAAGGGGGGA 
ATTCGCCTCA CCATAAAAGG ATCAAATTTA GGAATAAAAC CTGCTGATGT TGAAGAAATA 
ATAGTGGCAA ACACCCCGTG TACATTTATT GAAGAATTGT ATTCTGTCTC CACTAGTGTT 
GTTTGTGAAG TTGGTGCTGT GGACGAACCT GATTCAGGGA ACGTTAAAAT AAAGATTAAG 
GAGAAGATGG GAATATCGAG TCAGATTTTT ACATACCAGG ATCCAAACCC AGATTATCTA 
CACCCACTTG AAGGTATAAT GGCTGGTGGT ACTCGGTTGA CAATTAAGGG CCAAAGACTA 
GAAACAGGAT CCGAGAATGA TATCAGAGTG ACCCTTGATA GCATACCTTG TGCCGTTAAA 
TCTTTTGGTG AAGAAATTGT TTGTGATACA GGAATGGCTT CCAAAAAAGG AGAGGTGGCA 
GTAAAGCTGT TGTATGGCGA CCGCACAGAA ATAAATGCTG GACACTTTAT ATACAAGGAA 
AATCCATCAA TTGCTCATTT TACTCCAGAC AGAAGCTTTG CCAGTGGTGG GAGACATATT 
ACAATTTCAG GGTCAGGATT TAACCTTGTA CAGAATTTCA GAATAATTGC AAGACTGCAG 
GGAGAAGACA GTTCTTCGGT TACAGAAGAT GAATCCAAAA CTATTTCACA GGAAGACATC 
CTTGAAAAAG TAAATGACTC TGTGATTATA TTCACGTCTC CACAAGTGCC AGAAAATTAC 
CTGACCTCTG GGGTTACAAT TGTGATTAAA ATGGATGGAC TTGAGTCACC TCTTCAGACT 
AATGAACTTT CTTTCTTGTA CATCCCGGAT CCTACATTTG AGAACTTCAC TGATGGTATT 
AAGAAGCAAG TGAATAATCT CATAAATGCT AAGGGTGATA ATCTTAATGC TGCAATGACA 
ATTGAAGAGG CCAAAGCATT TGTGGGTGAA GGCACCTGCA AAATAAATAC TCTAACATCT 
ACTGACTTGT ACTGTGTCCC CCCAGAAGAA CAACCCCCAC CAAAGAGGCG TCACAAAAGA 
GATACAGCAA ATAACTTACC AGAGTTCATA GTGAAATTTG GAAAGCGTGA ATGGGTCCTG 
GGAAAAGTTG AATATGGACA ATCCCCTGCT ATTCCTCTTC ACATTATTAT TCCTGTTGTT 
ATCATTCCAA TGCTTCTAAT CATTGGAATT GCAGTTTACT TTTACAGGCG GAAAAGTCAA 
CAAGCAGAGC GTGAGTATGA AAAAGTCAAA CTGCAGCTCG AGGGCTTGGA AGAGACTGTG 
AGAGAGCGCT GCAAGAGGGA ATTTACAGAA CTAATGATTG AGATGGAAGA TCAAACCAGT 
GATCTCAGTG AGGCCGGCAT CCCTTTCCTA GATTACAAAA CATACACAGA CAGGGTGTTC 
TTCCTGTCAT CCAAAGATGG GGAAAAAGAT GTGATGATAA CTGGAAAGCT GGATATCCCA 
GAGGCCAGGC GGCAAACTGT AGAAGTAGCT CTGAACCAGT TCTCGAACCT TCTTAACAGT 
AAATCATTCC TTACAATTTT CATTCACACT CTAGAAAGCC AAAAGGACTT CTCTGCCAGA 
GAAAAACTCT ATTTAGCCTC CCTGCTAACT GTGGCTTTGC ATGGGAAACT GGAATATTAC 
ACTGATATCA TGAGAGCATT ATTAATGGAG TTAATGGACC AATATGTGGC CAAAAACCCC 
AAGCTTCTCC TTCGCAGGTC AGAAACTGTT GTGGAGCGGA TGCTTTCAAA TTGGATGTCC 
ATATGTTTAT ACCAGTATTT GAAAGACACT GTTGGTGAGC CGTTATACAA ACTCTTCAAA 
GCAATTAAAC ACCAAGTGGA AAAGGGCCCA GTTGATGCTT TGCAAGCTAA AGCCAAATAC 
ACACTCAATG ATACAGGATT ACTAGGGGAT GATGTTGAGT ACAAACAGTT GACCGTCAAC 
GTAATTGTTC AAGAGGAAGG TGCAGAACCA GTACCAGTAA AAGTGCTGAA CTGTGACACA 
ATTACCCAAG TAAAGGAGAA GATTGTTGAT CAGGTTTACA GGAACATTCC TTATTCCTTA 
CGGCCTAAGG CTGACAGTTT GGCTCTGGAA TGGAGGCCAG GGTCTACTGC ACAAATACTC 
TCTGATCTGG ATTTAACATC ACAGAAAGAA GGAAGGTTGA AACGCTATAA CACACTCATG 
CACTACAATG TCAGGGATAA TGCCACTCTC ATTCTATCAA GAATGGGGAT TTCTCAGCAG 
CAAGAGGAAA ACCACCAAGA CTTTCCAGGG GAAAGAAATG CATTGTTAGA GGATGAAAAT 
AAAGCCTGGC ACCTTGTCCG TCCAGCTGAT GAGGTAGATG AAGTCAAATC AAAGAGAGGA 
AGCATGAAAG AAAAGGAAAG AACCAAAGCA ATAACAGAAA TATATCTAAC TAGGCTGCTT 
TCTGTCAAGG GTACACTCCA GCAGTTTGTA GATAACTTCT TCCAGAGTGT CCTTAATTCC 
AATCAAGTGG TACCACCAGC TGTGAAATAC TTCTTCGATT TCCTTGATGA GCAAGCAGAA 
AAATATGAAA TCAAAGATGA AGATACTGTC CACATATGGA AAACAAACAG TTTATCACTA 
AGATTTTGGG TGAATATACT GAAAAATCCA CACTTTATTT TTGATGTGCA TGTTCACCAA 
GTAGTAGATG CCTCCTTGTC AGTCATCGCA CAGACTTTCA TGGATGCCTG CAGCAGGACG 
GAGCACAAGC TTAGCAGAGA ATCTCCAAGC AACAAGCTCT TGTATGCAAA AGAGATCTCA 
ACTTACAAAA AAATGGTGGA AGACTATTAC AAGGGAATCC GCCAGATGGT ACAAGTTAGC 
GACCAGGATA TGAATACACA TTTAGCAGAA ATTTCAAGGG CTCACACGGA GTCATTAAAT 
ACACTAGTAG CACTACATCA GCTGTATCAG TATACAAATA AATACTACGA TGAGATAATA 
AACGCTCTGG AGGAGGATCC TGCAGCCCAG CGCATGCAGT TAGCATATCG GCTACAGCAA 
ATAGCCGCAG CACTAGAGAA CAAAGTGACA GATCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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plxnb2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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