scn8a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	scn8a
Entrez Gene ID	549640
Full Name	sodium channel, voltage gated, type VIII alpha subunit
Synonyms	ceriii,na(v)1.6,nach6,nav1.6
General protein information	Preferred Names sodium channel, voltage gated, type VIII alpha subunit Names sodium channel, voltage gated, type VIII alpha subunit sodium channel, voltage-gated, type 8, alpha polypeptide voltage-dependent sodium channel SCN8A-like protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031895985.1	XP_031751845.1	sodium channel protein type 8 subunit alpha isoform X1
XM_031895986.1	XP_031751846.1	sodium channel protein type 8 subunit alpha isoform X2
NM_001016886.2	NP_001016886.1	sodium channel protein type 8 subunit alpha isoform 2
NM_001374728.1	NP_001361657.1	sodium channel, voltage gated, type VIII alpha subunit isoform 1
XM_031895987.1	XP_031751847.1	sodium channel protein type 8 subunit alpha isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Expansion of voltage-dependent Na+ channel gene family in early tetrapods coincided with the emergence of terrestriality and increased brain complexity.
Zakon HH, Jost MC, Lu Y
Molecular biology and evolution28(4)1415-24(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following scn8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scn8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21589	NM_001016886.2 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OXa47271	NM_001374728.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51381	XM_031895985.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51382	XM_031895986.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51383	XM_031895987.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scn8a ( NM_001016886.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016886.1 Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OXa21589
Clone ID Related Accession (Same CDS sequence)	NM_001016886.2
Accession Version	NM_001016886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 8 subunit alpha isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR848577.2. On Oct 14, 2005 this sequence version replaced NM_001016886.1. ##Evidence-Data-START## Transcript exon combination :: CR848577.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCATATGT GACAGAGTTT GTGGACCTTG GGAATGTCTC AGCGCTGAGA 
ACATTCAGAG TTCTCCGAGC TTTGAAAACT ATCTCTGTCA TTCCAGATGT TCTGTTGCAT 
GGCAGAATCA GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001016886.1
CDS	123..860
Translation	MAAPLMAPPGPDSFKAFTRESLAAIEKRIAEEKNKKPPKQDSSHRDDDDENKPRPNSDLEAGKGVPFIYGDCPSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPDVLLHGRIST

Target ORF information:

RefSeq Version	NM_001016886.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001016886.2

ORF Insert Sequence:

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCATATGT GACAGAGTTT GTGGACCTTG GGAATGTCTC AGCGCTGAGA 
ACATTCAGAG TTCTCCGAGC TTTGAAAACT ATCTCTGTCA TTCCAGATGT TCTGTTGCAT 
GGCAGAATCA GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn8a ( NM_001374728.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001361657.1 Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OXa47271
Clone ID Related Accession (Same CDS sequence)	NM_001374728.1
Accession Version	NM_001374728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-12
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel, voltage gated, type VIII alpha subunit isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03008918.1, AAMC03008917.1 and AAMC03008916.1.

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCATATGT GACAGAGTTT GTGGACCTTG GGAATGTCTC AGCGCTGAGA 
ACATTCAGAG TTCTCCGAGC TTTGAAAACT ATCTCTGTCA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGCCTA CAACAGAAGT AGAAGTGAAA AGGAAGCCTT CAGGATCTCT TCGTGTGTCC 
ATGGACCAGT TGACTGCATC TTTTGGAAGG AAGAGAGCCA ATAGTATTCT GAGTGTTGTA 
ACTCAGACTT TAGTGGAAGA GCTGGAGGAA TCACAAAGAA AGTGTCCCCC TTGTTGGTAC 
AAATTCGCAG ACACCTATCT AATATGGACT TGCTGTCCTT TGTGGATTCG CATTAAAACC 
ATTGTGAACA TGATTGTAAT GGATCCCTTT GTGGACTTGA CCATCACCAT TTGTATTGTT 
CTTAACACTT TATTTATGGC TATGGAACAC TATCCAATGA CACCACATTT TGAAAATGTT 
TTGGTTGTGG GAAATCTGGT TTTCACTGGC ATTTTTACAG CAGAAATGTT CTTGAAGTTG 
ATAGCGATGG ATCCATATTA CTATTTTCAG GAGGGCTGGA ACATATTTGA TGGTTTTATT 
GTTTCGCTCA GTTTAATGGA ATTAGGACTT CAGGACGTTG AGGGGCTTTC TGTGCTGCGC 
TCCTTCCGCT TGCTGAGAGT CTTCAAACTA GCTAAATCAT GGCCGACATT AAACATGCTG 
ATAAAAATCA TTGGGAATTC TGTTGGTGCC TTGGGGAACT TGACCTTGGT GCTTGCCATT 
ATCGTTTTCA TTTTTGCTGT AGTGGGCATG CAGCTGTTTG GCAAAAGCTA TAAGGACTGT 
GTCTGCAAGA TAAACCCAGA ATGTGTATTG CCACGCTGGC ACATGAATGA TTTTTTTCAT 
TCCTTCCTTA TTGTTTTCCG GGTATTATGT GGAGAGTGGA TTGAAACCAT GTGGGACTGC 
ATGGAGGTTG CTGGACAAGC CATGTGTATT ATTGTCTTCA TGATGGTGAT GGTCATTGGT 
AATCTGGTGG TGCTGAACCT TTTTCTGGCT TTACTGCTGA GCTCATTTAG CGCAGACAAC 
CTGGCAGCTA CAGATGATGA TGGGGAGATG AACAACCTGC AGATATCTGT CATTCGAATC 
AAAAAAGGGA TTGCTTGGAT TAAGCTCAAA GCGCATGATT TTATGCAAAA GCATTTTAAA 
AAGAAAGACG CCGATGAAGT TAAACCTCTG GATGAAATGT ATGAGAAGAA GATGAACTGT 
TTGGCAAACA ATACAGGTGC TGATATCCAC AGAGATATGG ATTACCCAAA GAATGGAAAT 
GGAACTACCA GTGGCATTGG GAGTAGTGTG GAAAAATACA TGATAGATGA GGATCACATG 
TCTTTCATAC ATAACCCTAA CCTCACCGTT AAAGTGCCCA TTGCAGTTGG AGAGTCAGAT 
TTTGAAAATC TTAACACGGA GGATTTCAGC AGTGCGTCTG ATGAAGAAGG CAGCAAAGAT 
AAACTGGATG ACACCAGTTC TTCAGAAGGA AGTACAATTG ATATAAAACC AGAAGCTGAG 
GAAGTTCCTG TAGAGCAACC AGAAGAGTAT TTAGATCCGG ATAACTGCTT TACTGAAGGC 
TGTGTGGCTC GTTTTAAGTG TTGCCAAGTC AGTGTGGAAG AAGGTTTGGG TAAAAGTTGG 
TGGACACTGC GGAAAACCTG TTTCCTCATA GTGGAGCACA ACTGGTTTGA AACCTTCATC 
ATTTTCATGA TATTGTTAAG CAGTGGAGCG CTGGCCTTTG AGGATGTTTA CATAGAACAG 
CGGAAAACCA TTCGAACGAT CCTTGAATAT GCAGATAAAG TTTTCACTTA CATTTTCATA 
TTGGAAATGT TGCTGAAATG GTTGGCATAT GGATTTGTGA AGTTCTTCAC TAATGCCTGG 
TGTTGGCTAG ACTTCCTCAT TGTTGATGTC TCTTTAGTAA GCCTTATAGC TAATGCCCTG 
GGCTACTCGG AACTAGGTGC CATTAAGTCC CTTAGGACCT TAAGAGCATT GAGACCCCTA 
AGAGCCTTGT CACGATTTGA AGGGATGAGG GTGGTAGTTA ATGCCTTGGT TGGTGCCATC 
CCTTCAATTA TGAATGTGTT GCTGGTCTGC CTTATATTTT GGCTGATTTT TAGTATCATG 
GGTGTTAACC TGTTTGCGGG CAAGTACTAC TACTGCTATA ACGAAACGGC AGAAGGCATG 
TTTGAAATTG AAGACGTGGA AAATGAAACA CAATGTTTTG ACCTTATTGG AAATAATTAT 
ACCGAAGTTA GATGGAAAAA TGTGAAAATC AACTTTGACA ATGTTGGAGC TGGATATCTT 
GCATTGCTTC AAGTAGCTAC TTTTAAAGGC TGGATGGATA TTATGTATGC AGCAGTAGAC 
TCCAGGAAGG TTCACCAGCA ACCAAAATAT GAGGACAACA TTTATATGTA CATTTATTTT 
GTAATCTTCA TTATTTTTGG CTCTTTCTTC ACTCTAAATC TGTTTATTGG TGTCATCATT 
GATAACTTCA ATCAACAAAA GAAAAAGTTT GGAGGTCAAG ACATCTTCAT GACAGAAGAA 
CAAAAAAAGT ATTATAATGC TATGAAGAAA CTGGGCTCTA AGAAACCACA AAAACCCATA 
CCCAGGCCAC TAAATAAATT CCAAGGAGCC ATTTTTGATA TAATCACACA ACAAGCATTT 
GATATTGTGA TTATGATTCT GATTTGTCTT AACATGGTGA CAATGATGGT AGAAACCGAT 
GACCAGAGTG ATTACACAGA TAATGTTCTC TACTGGATCA ATGTTGTTTT CATTGTATTT 
TTTACCACTG AGTGTGTGCT AAAGCTGTGT GCACTGCGGC ATTATTACTT TACCATCGGA 
TGGAATATTT TCGACTTTGT TGTTGTAATC CTTTCAATTG TTGGAATGTT CCTGGCTGAT 
TTGATCACAA AATACTTTGT GTCACCCACT TTGTTCAGAG TCATTCGTCT GGCTCGTATT 
GGCCGCATAC TTCGCTTAAT CAAGGGAGCC AAAGGGATAC GGACCCTGCT TTTTGCCTTA 
ATGATGTCAT TGCCGGCTTT GTTCAACATC GGCCTTCTTC TGTTTCTAGT GATGTTTATA 
TTTTCAATAT TTGGTATGTC GAACTTCGCT TATGTGAAGA AGGAAGCAGG GATAGATGAC 
ATGTTCAACT TTGAAACCTT TGGCAATAGT ATGATCTGCT TATTTCAGAT CACTACATCT 
GCTGGCTGGG ATGGCTTGTT GCTGCCTATC CTAAATCGTC CGCCAGATTG TGATGCGCAC 
AAAGAGAATC CAGGTAGTCA AGTGAAAGGT GACTGCGGAA ATCCTTCTGT TGGCATCTTT 
TTCTTTGTCA GCTACATTAT AATCTCTTTC CTGATTGTAG TGAACATGTA CATTGCTATC 
ATTCTGGAAA ATTTCAGCGT GGCAACTGAA GAGAGTGCTG ATCCTTTAAG TGAGGATGAC 
TTTGAAACCT TTTATGAAAT TTGGGAGAAA TTTGATCCAG ATGCAACACA GTTTATCCAG 
TTCAGCAAAC TGCCAGATTT CGCAGATGCC TTGGAGCATC CACTGCGAAT CCCCAAGCCA 
AACACCATTG AACTAATTGC AATGGATTTG CCGATGGTGA GTGGTGACAG AATTCATTGT 
CTGGATATCC TATTTGCCTT CACCAAACGG GTACTTGGAG AGAGCGGAGA GCTGGATATA 
TTGCGCCAGC AGATGGAAGA GAGATTTGTG GCTTCAAACC CTTCAAAAGT GTCTTATGAG 
CCCATTACCA CCACACTCAG GCGAAAGCAA GAGGATGTAT CTGCGGTTGT TATCCAGCAA 
GCTTACAGGC GCCATTTAGT CAAACGAGGG TTTATTTCTA GAAAGCGTTC AACTGGCAAA 
ATGGAGAATG GGGGAACCAA CATGGATAAA AAAGAGGGAA CGCCATCTAC TGCATCACTC 
CCATCATATG ACAGTGTAAC TAAACCTGAC AAAGCAAAAC AAGAAAGAAT AGAAGATGCA 
ATTAGAGAGA GAGCTAAAAA ACAAAAATCT GTCAGGGAAT CAAAGTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361657.1
CDS	276..6206
Translation	MAAPLMAPPGPDSFKAFTRESLAAIEKRIAEEKNKKPPKQDSSHRDDDDENKPRPNSDLEAGKGVPFIYGDCPSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCVAWPVDMDQMYLANGSKGFDFDEYIMNDTNFFKVHGAIDPLLCGNGSDAGQCPEGYTCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAERKENEFKAMLEQMKRMQEEAQAAANATSAGTVTEDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKELSEGEEKGDSEKVYKSESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSRHNSKSSIFSFRGRFRDPVSENEFADDEHSTVEESEGRRDSLFIPVRGHDRRNSYNSGYSQGSRSSRILQNLRRNPKRNSTVDCNGVVSLIGGPGSNIPGGRLLPEPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWIRIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLQDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMNCLANNTGADIHRDMDYPKNGNGTTSGIGSSVEKYMIDEDHMSFIHNPNLTVKVPIAVGESDFENLNTEDFSSASDEEGSKDKLDDTSSSEGSTIDIKPEAEEVPVEQPEEYLDPDNCFTEGCVARFKCCQVSVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGESGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEDVSAVVIQQAYRRHLVKRGFISRKRSTGKMENGGTNMDKKEGTPSTASLPSYDSVTKPDKAKQERIEDAIRERAKKQKSVRESKC

Target ORF information:

RefSeq Version	NM_001374728.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374728.1

ORF Insert Sequence:

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCATATGT GACAGAGTTT GTGGACCTTG GGAATGTCTC AGCGCTGAGA 
ACATTCAGAG TTCTCCGAGC TTTGAAAACT ATCTCTGTCA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGCCTA CAACAGAAGT AGAAGTGAAA AGGAAGCCTT CAGGATCTCT TCGTGTGTCC 
ATGGACCAGT TGACTGCATC TTTTGGAAGG AAGAGAGCCA ATAGTATTCT GAGTGTTGTA 
ACTCAGACTT TAGTGGAAGA GCTGGAGGAA TCACAAAGAA AGTGTCCCCC TTGTTGGTAC 
AAATTCGCAG ACACCTATCT AATATGGACT TGCTGTCCTT TGTGGATTCG CATTAAAACC 
ATTGTGAACA TGATTGTAAT GGATCCCTTT GTGGACTTGA CCATCACCAT TTGTATTGTT 
CTTAACACTT TATTTATGGC TATGGAACAC TATCCAATGA CACCACATTT TGAAAATGTT 
TTGGTTGTGG GAAATCTGGT TTTCACTGGC ATTTTTACAG CAGAAATGTT CTTGAAGTTG 
ATAGCGATGG ATCCATATTA CTATTTTCAG GAGGGCTGGA ACATATTTGA TGGTTTTATT 
GTTTCGCTCA GTTTAATGGA ATTAGGACTT CAGGACGTTG AGGGGCTTTC TGTGCTGCGC 
TCCTTCCGCT TGCTGAGAGT CTTCAAACTA GCTAAATCAT GGCCGACATT AAACATGCTG 
ATAAAAATCA TTGGGAATTC TGTTGGTGCC TTGGGGAACT TGACCTTGGT GCTTGCCATT 
ATCGTTTTCA TTTTTGCTGT AGTGGGCATG CAGCTGTTTG GCAAAAGCTA TAAGGACTGT 
GTCTGCAAGA TAAACCCAGA ATGTGTATTG CCACGCTGGC ACATGAATGA TTTTTTTCAT 
TCCTTCCTTA TTGTTTTCCG GGTATTATGT GGAGAGTGGA TTGAAACCAT GTGGGACTGC 
ATGGAGGTTG CTGGACAAGC CATGTGTATT ATTGTCTTCA TGATGGTGAT GGTCATTGGT 
AATCTGGTGG TGCTGAACCT TTTTCTGGCT TTACTGCTGA GCTCATTTAG CGCAGACAAC 
CTGGCAGCTA CAGATGATGA TGGGGAGATG AACAACCTGC AGATATCTGT CATTCGAATC 
AAAAAAGGGA TTGCTTGGAT TAAGCTCAAA GCGCATGATT TTATGCAAAA GCATTTTAAA 
AAGAAAGACG CCGATGAAGT TAAACCTCTG GATGAAATGT ATGAGAAGAA GATGAACTGT 
TTGGCAAACA ATACAGGTGC TGATATCCAC AGAGATATGG ATTACCCAAA GAATGGAAAT 
GGAACTACCA GTGGCATTGG GAGTAGTGTG GAAAAATACA TGATAGATGA GGATCACATG 
TCTTTCATAC ATAACCCTAA CCTCACCGTT AAAGTGCCCA TTGCAGTTGG AGAGTCAGAT 
TTTGAAAATC TTAACACGGA GGATTTCAGC AGTGCGTCTG ATGAAGAAGG CAGCAAAGAT 
AAACTGGATG ACACCAGTTC TTCAGAAGGA AGTACAATTG ATATAAAACC AGAAGCTGAG 
GAAGTTCCTG TAGAGCAACC AGAAGAGTAT TTAGATCCGG ATAACTGCTT TACTGAAGGC 
TGTGTGGCTC GTTTTAAGTG TTGCCAAGTC AGTGTGGAAG AAGGTTTGGG TAAAAGTTGG 
TGGACACTGC GGAAAACCTG TTTCCTCATA GTGGAGCACA ACTGGTTTGA AACCTTCATC 
ATTTTCATGA TATTGTTAAG CAGTGGAGCG CTGGCCTTTG AGGATGTTTA CATAGAACAG 
CGGAAAACCA TTCGAACGAT CCTTGAATAT GCAGATAAAG TTTTCACTTA CATTTTCATA 
TTGGAAATGT TGCTGAAATG GTTGGCATAT GGATTTGTGA AGTTCTTCAC TAATGCCTGG 
TGTTGGCTAG ACTTCCTCAT TGTTGATGTC TCTTTAGTAA GCCTTATAGC TAATGCCCTG 
GGCTACTCGG AACTAGGTGC CATTAAGTCC CTTAGGACCT TAAGAGCATT GAGACCCCTA 
AGAGCCTTGT CACGATTTGA AGGGATGAGG GTGGTAGTTA ATGCCTTGGT TGGTGCCATC 
CCTTCAATTA TGAATGTGTT GCTGGTCTGC CTTATATTTT GGCTGATTTT TAGTATCATG 
GGTGTTAACC TGTTTGCGGG CAAGTACTAC TACTGCTATA ACGAAACGGC AGAAGGCATG 
TTTGAAATTG AAGACGTGGA AAATGAAACA CAATGTTTTG ACCTTATTGG AAATAATTAT 
ACCGAAGTTA GATGGAAAAA TGTGAAAATC AACTTTGACA ATGTTGGAGC TGGATATCTT 
GCATTGCTTC AAGTAGCTAC TTTTAAAGGC TGGATGGATA TTATGTATGC AGCAGTAGAC 
TCCAGGAAGG TTCACCAGCA ACCAAAATAT GAGGACAACA TTTATATGTA CATTTATTTT 
GTAATCTTCA TTATTTTTGG CTCTTTCTTC ACTCTAAATC TGTTTATTGG TGTCATCATT 
GATAACTTCA ATCAACAAAA GAAAAAGTTT GGAGGTCAAG ACATCTTCAT GACAGAAGAA 
CAAAAAAAGT ATTATAATGC TATGAAGAAA CTGGGCTCTA AGAAACCACA AAAACCCATA 
CCCAGGCCAC TAAATAAATT CCAAGGAGCC ATTTTTGATA TAATCACACA ACAAGCATTT 
GATATTGTGA TTATGATTCT GATTTGTCTT AACATGGTGA CAATGATGGT AGAAACCGAT 
GACCAGAGTG ATTACACAGA TAATGTTCTC TACTGGATCA ATGTTGTTTT CATTGTATTT 
TTTACCACTG AGTGTGTGCT AAAGCTGTGT GCACTGCGGC ATTATTACTT TACCATCGGA 
TGGAATATTT TCGACTTTGT TGTTGTAATC CTTTCAATTG TTGGAATGTT CCTGGCTGAT 
TTGATCACAA AATACTTTGT GTCACCCACT TTGTTCAGAG TCATTCGTCT GGCTCGTATT 
GGCCGCATAC TTCGCTTAAT CAAGGGAGCC AAAGGGATAC GGACCCTGCT TTTTGCCTTA 
ATGATGTCAT TGCCGGCTTT GTTCAACATC GGCCTTCTTC TGTTTCTAGT GATGTTTATA 
TTTTCAATAT TTGGTATGTC GAACTTCGCT TATGTGAAGA AGGAAGCAGG GATAGATGAC 
ATGTTCAACT TTGAAACCTT TGGCAATAGT ATGATCTGCT TATTTCAGAT CACTACATCT 
GCTGGCTGGG ATGGCTTGTT GCTGCCTATC CTAAATCGTC CGCCAGATTG TGATGCGCAC 
AAAGAGAATC CAGGTAGTCA AGTGAAAGGT GACTGCGGAA ATCCTTCTGT TGGCATCTTT 
TTCTTTGTCA GCTACATTAT AATCTCTTTC CTGATTGTAG TGAACATGTA CATTGCTATC 
ATTCTGGAAA ATTTCAGCGT GGCAACTGAA GAGAGTGCTG ATCCTTTAAG TGAGGATGAC 
TTTGAAACCT TTTATGAAAT TTGGGAGAAA TTTGATCCAG ATGCAACACA GTTTATCCAG 
TTCAGCAAAC TGCCAGATTT CGCAGATGCC TTGGAGCATC CACTGCGAAT CCCCAAGCCA 
AACACCATTG AACTAATTGC AATGGATTTG CCGATGGTGA GTGGTGACAG AATTCATTGT 
CTGGATATCC TATTTGCCTT CACCAAACGG GTACTTGGAG AGAGCGGAGA GCTGGATATA 
TTGCGCCAGC AGATGGAAGA GAGATTTGTG GCTTCAAACC CTTCAAAAGT GTCTTATGAG 
CCCATTACCA CCACACTCAG GCGAAAGCAA GAGGATGTAT CTGCGGTTGT TATCCAGCAA 
GCTTACAGGC GCCATTTAGT CAAACGAGGG TTTATTTCTA GAAAGCGTTC AACTGGCAAA 
ATGGAGAATG GGGGAACCAA CATGGATAAA AAAGAGGGAA CGCCATCTAC TGCATCACTC 
CCATCATATG ACAGTGTAAC TAAACCTGAC AAAGCAAAAC AAGAAAGAAT AGAAGATGCA 
ATTAGAGAGA GAGCTAAAAA ACAAAAATCT GTCAGGGAAT CAAAGTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn8a ( XM_031895985.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751845.1 Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X1, mRNA.

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CloneID	OXa51381
Clone ID Related Accession (Same CDS sequence)	XM_031895985.1
Accession Version	XM_031895985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 8 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCATATGT GACAGAGTTT GTGGACCTTG GGAATGTCTC AGCGCTGAGA 
ACATTCAGAG TTCTCCGAGC TTTGAAAACT ATCTCTGTCA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGGTGA AAATAGATAA GGCAGCTACT GATGACAGCC CTACAACAGA AGTAGAAGTG 
AAAAGGAAGC CTTCAGGATC TCTTCGTGTG TCCATGGACC AGTTGACTGC ATCTTTTGGA 
AGGAAGAGAG CCAATAGTAT TCTGAGTGTT GTAACTCAGA CTTTAGTGGA AGAGCTGGAG 
GAATCACAAA GAAAGTGTCC CCCTTGTTGG TACAAATTCG CAGACACCTA TCTAATATGG 
ACTTGCTGTC CTTTGTGGAT TCGCATTAAA ACCATTGTGA ACATGATTGT AATGGATCCC 
TTTGTGGACT TGACCATCAC CATTTGTATT GTTCTTAACA CTTTATTTAT GGCTATGGAA 
CACTATCCAA TGACACCACA TTTTGAAAAT GTTTTGGTTG TGGGAAATCT GGTTTTCACT 
GGCATTTTTA CAGCAGAAAT GTTCTTGAAG TTGATAGCGA TGGATCCATA TTACTATTTT 
CAGGAGGGCT GGAACATATT TGATGGTTTT ATTGTTTCGC TCAGTTTAAT GGAATTAGGA 
CTTCAGGACG TTGAGGGGCT TTCTGTGCTG CGCTCCTTCC GCTTGCTGAG AGTCTTCAAA 
CTAGCTAAAT CATGGCCGAC ATTAAACATG CTGATAAAAA TCATTGGGAA TTCTGTTGGT 
GCCTTGGGGA ACTTGACCTT GGTGCTTGCC ATTATCGTTT TCATTTTTGC TGTAGTGGGC 
ATGCAGCTGT TTGGCAAAAG CTATAAGGAC TGTGTCTGCA AGATAAACCC AGAATGTGTA 
TTGCCACGCT GGCACATGAA TGATTTTTTT CATTCCTTCC TTATTGTTTT CCGGGTATTA 
TGTGGAGAGT GGATTGAAAC CATGTGGGAC TGCATGGAGG TTGCTGGACA AGCCATGTGT 
ATTATTGTCT TCATGATGGT GATGGTCATT GGTAATCTGG TGGTGCTGAA CCTTTTTCTG 
GCTTTACTGC TGAGCTCATT TAGCGCAGAC AACCTGGCAG CTACAGATGA TGATGGGGAG 
ATGAACAACC TGCAGATATC TGTCATTCGA ATCAAAAAAG GGATTGCTTG GATTAAGCTC 
AAAGCGCATG ATTTTATGCA AAAGCATTTT AAAAAGAAAG ACGCCGATGA AGTTAAACCT 
CTGGATGAAA TGTATGAGAA GAAGATGAAC TGTTTGGCAA ACAATACAGG TGCTGATATC 
CACAGAGATA TGGATTACCC AAAGAATGGA AATGGAACTA CCAGTGGCAT TGGGAGTAGT 
GTGGAAAAAT ACATGATAGA TGAGGATCAC ATGTCTTTCA TACATAACCC TAACCTCACC 
GTTAAAGTGC CCATTGCAGT TGGAGAGTCA GATTTTGAAA ATCTTAACAC GGAGGATTTC 
AGCAGTGCGT CTGATGAAGA AGGCAGCAAA GATAAACTGG ATGACACCAG TTCTTCAGAA 
GGAAGTACAA TTGATATAAA ACCAGAAGCT GAGGAAGTTC CTGTAGAGCA ACCAGAAGAG 
TATTTAGATC CGGATAACTG CTTTACTGAA GGCTGTGTGG CTCGTTTTAA GTGTTGCCAA 
GTCAGTGTGG AAGAAGGTTT GGGTAAAAGT TGGTGGACAC TGCGGAAAAC CTGTTTCCTC 
ATAGTGGAGC ACAACTGGTT TGAAACCTTC ATCATTTTCA TGATATTGTT AAGCAGTGGA 
GCGCTGGCCT TTGAGGATGT TTACATAGAA CAGCGGAAAA CCATTCGAAC GATCCTTGAA 
TATGCAGATA AAGTTTTCAC TTACATTTTC ATATTGGAAA TGTTGCTGAA ATGGTTGGCA 
TATGGATTTG TGAAGTTCTT CACTAATGCC TGGTGTTGGC TAGACTTCCT CATTGTTGAT 
GTCTCTTTAG TAAGCCTTAT AGCTAATGCC CTGGGCTACT CGGAACTAGG TGCCATTAAG 
TCCCTTAGGA CCTTAAGAGC ATTGAGACCC CTAAGAGCCT TGTCACGATT TGAAGGGATG 
AGGGTGGTAG TTAATGCCTT GGTTGGTGCC ATCCCTTCAA TTATGAATGT GTTGCTGGTC 
TGCCTTATAT TTTGGCTGAT TTTTAGTATC ATGGGTGTTA ACCTGTTTGC GGGCAAGTAC 
TACTACTGCT ATAACGAAAC GGCAGAAGGC ATGTTTGAAA TTGAAGACGT GGAAAATGAA 
ACACAATGTT TTGACCTTAT TGGAAATAAT TATACCGAAG TTAGATGGAA AAATGTGAAA 
ATCAACTTTG ACAATGTTGG AGCTGGATAT CTTGCATTGC TTCAAGTAGC TACTTTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAGTA GACTCCAGGA AGGTTCACCA GCAACCAAAA 
TATGAGGACA ACATTTATAT GTACATTTAT TTTGTAATCT TCATTATTTT TGGCTCTTTC 
TTCACTCTAA ATCTGTTTAT TGGTGTCATC ATTGATAACT TCAATCAACA AAAGAAAAAG 
TTTGGAGGTC AAGACATCTT CATGACAGAA GAACAAAAAA AGTATTATAA TGCTATGAAG 
AAACTGGGCT CTAAGAAACC ACAAAAACCC ATACCCAGGC CACTAAATAA ATTCCAAGGA 
GCCATTTTTG ATATAATCAC ACAACAAGCA TTTGATATTG TGATTATGAT TCTGATTTGT 
CTTAACATGG TGACAATGAT GGTAGAAACC GATGACCAGA GTGATTACAC AGATAATGTT 
CTCTACTGGA TCAATGTTGT TTTCATTGTA TTTTTTACCA CTGAGTGTGT GCTAAAGCTG 
TGTGCACTGC GGCATTATTA CTTTACCATC GGATGGAATA TTTTCGACTT TGTTGTTGTA 
ATCCTTTCAA TTGTTGGAAT GTTCCTGGCT GATTTGATCA CAAAATACTT TGTGTCACCC 
ACTTTGTTCA GAGTCATTCG TCTGGCTCGT ATTGGCCGCA TACTTCGCTT AATCAAGGGA 
GCCAAAGGGA TACGGACCCT GCTTTTTGCC TTAATGATGT CATTGCCGGC TTTGTTCAAC 
ATCGGCCTTC TTCTGTTTCT AGTGATGTTT ATATTTTCAA TATTTGGTAT GTCGAACTTC 
GCTTATGTGA AGAAGGAAGC AGGGATAGAT GACATGTTCA ACTTTGAAAC CTTTGGCAAT 
AGTATGATCT GCTTATTTCA GATCACTACA TCTGCTGGCT GGGATGGCTT GTTGCTGCCT 
ATCCTAAATC GTCCGCCAGA TTGTGATGCG CACAAAGAGA ATCCAGGTAG TCAAGTGAAA 
GGTGACTGCG GAAATCCTTC TGTTGGCATC TTTTTCTTTG TCAGCTACAT TATAATCTCT 
TTCCTGATTG TAGTGAACAT GTACATTGCT ATCATTCTGG AAAATTTCAG CGTGGCAACT 
GAAGAGAGTG CTGATCCTTT AAGTGAGGAT GACTTTGAAA CCTTTTATGA AATTTGGGAG 
AAATTTGATC CAGATGCAAC ACAGTTTATC CAGTTCAGCA AACTGCCAGA TTTCGCAGAT 
GCCTTGGAGC ATCCACTGCG AATCCCCAAG CCAAACACCA TTGAACTAAT TGCAATGGAT 
TTGCCGATGG TGAGTGGTGA CAGAATTCAT TGTCTGGATA TCCTATTTGC CTTCACCAAA 
CGGGTACTTG GAGAGAGCGG AGAGCTGGAT ATATTGCGCC AGCAGATGGA AGAGAGATTT 
GTGGCTTCAA ACCCTTCAAA AGTGTCTTAT GAGCCCATTA CCACCACACT CAGGCGAAAG 
CAAGAGGATG TATCTGCGGT TGTTATCCAG CAAGCTTACA GGCGCCATTT AGTCAAACGA 
GGGTTTATTT CTAGAAAGCG TTCAACTGGC AAAATGGAGA ATGGGGGAAC CAACATGGAT 
AAAAAAGAGG GAACGCCATC TACTGCATCA CTCCCATCAT ATGACAGTGT AACTAAACCT 
GACAAAGCAA AACAAGAAAG AATAGAAGAT GCAATTAGAG AGAGAGCTAA AAAACAAAAA 
TCTGTCAGGG AATCAAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751845.1
CDS	315..6278
Translation	MAAPLMAPPGPDSFKAFTRESLAAIEKRIAEEKNKKPPKQDSSHRDDDDENKPRPNSDLEAGKGVPFIYGDCPSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCVAWPVDMDQMYLANGSKGFDFDEYIMNDTNFFKVHGAIDPLLCGNGSDAGQCPEGYTCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAERKENEFKAMLEQMKRMQEEAQAAANATSAGTVTEDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKELSEGEEKGDSEKVYKSESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSRHNSKSSIFSFRGRFRDPVSENEFADDEHSTVEESEGRRDSLFIPVRGHDRRNSYNSGYSQGSRSSRILQNLRRNPKRNSTVDCNGVVSLIGGPGSNIPGGRLLPEVKIDKAATDDSPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWIRIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLQDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMNCLANNTGADIHRDMDYPKNGNGTTSGIGSSVEKYMIDEDHMSFIHNPNLTVKVPIAVGESDFENLNTEDFSSASDEEGSKDKLDDTSSSEGSTIDIKPEAEEVPVEQPEEYLDPDNCFTEGCVARFKCCQVSVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGESGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEDVSAVVIQQAYRRHLVKRGFISRKRSTGKMENGGTNMDKKEGTPSTASLPSYDSVTKPDKAKQERIEDAIRERAKKQKSVRESKC

Target ORF information:

RefSeq Version	XM_031895985.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895985.1

ORF Insert Sequence:

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCATATGT GACAGAGTTT GTGGACCTTG GGAATGTCTC AGCGCTGAGA 
ACATTCAGAG TTCTCCGAGC TTTGAAAACT ATCTCTGTCA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGGTGA AAATAGATAA GGCAGCTACT GATGACAGCC CTACAACAGA AGTAGAAGTG 
AAAAGGAAGC CTTCAGGATC TCTTCGTGTG TCCATGGACC AGTTGACTGC ATCTTTTGGA 
AGGAAGAGAG CCAATAGTAT TCTGAGTGTT GTAACTCAGA CTTTAGTGGA AGAGCTGGAG 
GAATCACAAA GAAAGTGTCC CCCTTGTTGG TACAAATTCG CAGACACCTA TCTAATATGG 
ACTTGCTGTC CTTTGTGGAT TCGCATTAAA ACCATTGTGA ACATGATTGT AATGGATCCC 
TTTGTGGACT TGACCATCAC CATTTGTATT GTTCTTAACA CTTTATTTAT GGCTATGGAA 
CACTATCCAA TGACACCACA TTTTGAAAAT GTTTTGGTTG TGGGAAATCT GGTTTTCACT 
GGCATTTTTA CAGCAGAAAT GTTCTTGAAG TTGATAGCGA TGGATCCATA TTACTATTTT 
CAGGAGGGCT GGAACATATT TGATGGTTTT ATTGTTTCGC TCAGTTTAAT GGAATTAGGA 
CTTCAGGACG TTGAGGGGCT TTCTGTGCTG CGCTCCTTCC GCTTGCTGAG AGTCTTCAAA 
CTAGCTAAAT CATGGCCGAC ATTAAACATG CTGATAAAAA TCATTGGGAA TTCTGTTGGT 
GCCTTGGGGA ACTTGACCTT GGTGCTTGCC ATTATCGTTT TCATTTTTGC TGTAGTGGGC 
ATGCAGCTGT TTGGCAAAAG CTATAAGGAC TGTGTCTGCA AGATAAACCC AGAATGTGTA 
TTGCCACGCT GGCACATGAA TGATTTTTTT CATTCCTTCC TTATTGTTTT CCGGGTATTA 
TGTGGAGAGT GGATTGAAAC CATGTGGGAC TGCATGGAGG TTGCTGGACA AGCCATGTGT 
ATTATTGTCT TCATGATGGT GATGGTCATT GGTAATCTGG TGGTGCTGAA CCTTTTTCTG 
GCTTTACTGC TGAGCTCATT TAGCGCAGAC AACCTGGCAG CTACAGATGA TGATGGGGAG 
ATGAACAACC TGCAGATATC TGTCATTCGA ATCAAAAAAG GGATTGCTTG GATTAAGCTC 
AAAGCGCATG ATTTTATGCA AAAGCATTTT AAAAAGAAAG ACGCCGATGA AGTTAAACCT 
CTGGATGAAA TGTATGAGAA GAAGATGAAC TGTTTGGCAA ACAATACAGG TGCTGATATC 
CACAGAGATA TGGATTACCC AAAGAATGGA AATGGAACTA CCAGTGGCAT TGGGAGTAGT 
GTGGAAAAAT ACATGATAGA TGAGGATCAC ATGTCTTTCA TACATAACCC TAACCTCACC 
GTTAAAGTGC CCATTGCAGT TGGAGAGTCA GATTTTGAAA ATCTTAACAC GGAGGATTTC 
AGCAGTGCGT CTGATGAAGA AGGCAGCAAA GATAAACTGG ATGACACCAG TTCTTCAGAA 
GGAAGTACAA TTGATATAAA ACCAGAAGCT GAGGAAGTTC CTGTAGAGCA ACCAGAAGAG 
TATTTAGATC CGGATAACTG CTTTACTGAA GGCTGTGTGG CTCGTTTTAA GTGTTGCCAA 
GTCAGTGTGG AAGAAGGTTT GGGTAAAAGT TGGTGGACAC TGCGGAAAAC CTGTTTCCTC 
ATAGTGGAGC ACAACTGGTT TGAAACCTTC ATCATTTTCA TGATATTGTT AAGCAGTGGA 
GCGCTGGCCT TTGAGGATGT TTACATAGAA CAGCGGAAAA CCATTCGAAC GATCCTTGAA 
TATGCAGATA AAGTTTTCAC TTACATTTTC ATATTGGAAA TGTTGCTGAA ATGGTTGGCA 
TATGGATTTG TGAAGTTCTT CACTAATGCC TGGTGTTGGC TAGACTTCCT CATTGTTGAT 
GTCTCTTTAG TAAGCCTTAT AGCTAATGCC CTGGGCTACT CGGAACTAGG TGCCATTAAG 
TCCCTTAGGA CCTTAAGAGC ATTGAGACCC CTAAGAGCCT TGTCACGATT TGAAGGGATG 
AGGGTGGTAG TTAATGCCTT GGTTGGTGCC ATCCCTTCAA TTATGAATGT GTTGCTGGTC 
TGCCTTATAT TTTGGCTGAT TTTTAGTATC ATGGGTGTTA ACCTGTTTGC GGGCAAGTAC 
TACTACTGCT ATAACGAAAC GGCAGAAGGC ATGTTTGAAA TTGAAGACGT GGAAAATGAA 
ACACAATGTT TTGACCTTAT TGGAAATAAT TATACCGAAG TTAGATGGAA AAATGTGAAA 
ATCAACTTTG ACAATGTTGG AGCTGGATAT CTTGCATTGC TTCAAGTAGC TACTTTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAGTA GACTCCAGGA AGGTTCACCA GCAACCAAAA 
TATGAGGACA ACATTTATAT GTACATTTAT TTTGTAATCT TCATTATTTT TGGCTCTTTC 
TTCACTCTAA ATCTGTTTAT TGGTGTCATC ATTGATAACT TCAATCAACA AAAGAAAAAG 
TTTGGAGGTC AAGACATCTT CATGACAGAA GAACAAAAAA AGTATTATAA TGCTATGAAG 
AAACTGGGCT CTAAGAAACC ACAAAAACCC ATACCCAGGC CACTAAATAA ATTCCAAGGA 
GCCATTTTTG ATATAATCAC ACAACAAGCA TTTGATATTG TGATTATGAT TCTGATTTGT 
CTTAACATGG TGACAATGAT GGTAGAAACC GATGACCAGA GTGATTACAC AGATAATGTT 
CTCTACTGGA TCAATGTTGT TTTCATTGTA TTTTTTACCA CTGAGTGTGT GCTAAAGCTG 
TGTGCACTGC GGCATTATTA CTTTACCATC GGATGGAATA TTTTCGACTT TGTTGTTGTA 
ATCCTTTCAA TTGTTGGAAT GTTCCTGGCT GATTTGATCA CAAAATACTT TGTGTCACCC 
ACTTTGTTCA GAGTCATTCG TCTGGCTCGT ATTGGCCGCA TACTTCGCTT AATCAAGGGA 
GCCAAAGGGA TACGGACCCT GCTTTTTGCC TTAATGATGT CATTGCCGGC TTTGTTCAAC 
ATCGGCCTTC TTCTGTTTCT AGTGATGTTT ATATTTTCAA TATTTGGTAT GTCGAACTTC 
GCTTATGTGA AGAAGGAAGC AGGGATAGAT GACATGTTCA ACTTTGAAAC CTTTGGCAAT 
AGTATGATCT GCTTATTTCA GATCACTACA TCTGCTGGCT GGGATGGCTT GTTGCTGCCT 
ATCCTAAATC GTCCGCCAGA TTGTGATGCG CACAAAGAGA ATCCAGGTAG TCAAGTGAAA 
GGTGACTGCG GAAATCCTTC TGTTGGCATC TTTTTCTTTG TCAGCTACAT TATAATCTCT 
TTCCTGATTG TAGTGAACAT GTACATTGCT ATCATTCTGG AAAATTTCAG CGTGGCAACT 
GAAGAGAGTG CTGATCCTTT AAGTGAGGAT GACTTTGAAA CCTTTTATGA AATTTGGGAG 
AAATTTGATC CAGATGCAAC ACAGTTTATC CAGTTCAGCA AACTGCCAGA TTTCGCAGAT 
GCCTTGGAGC ATCCACTGCG AATCCCCAAG CCAAACACCA TTGAACTAAT TGCAATGGAT 
TTGCCGATGG TGAGTGGTGA CAGAATTCAT TGTCTGGATA TCCTATTTGC CTTCACCAAA 
CGGGTACTTG GAGAGAGCGG AGAGCTGGAT ATATTGCGCC AGCAGATGGA AGAGAGATTT 
GTGGCTTCAA ACCCTTCAAA AGTGTCTTAT GAGCCCATTA CCACCACACT CAGGCGAAAG 
CAAGAGGATG TATCTGCGGT TGTTATCCAG CAAGCTTACA GGCGCCATTT AGTCAAACGA 
GGGTTTATTT CTAGAAAGCG TTCAACTGGC AAAATGGAGA ATGGGGGAAC CAACATGGAT 
AAAAAAGAGG GAACGCCATC TACTGCATCA CTCCCATCAT ATGACAGTGT AACTAAACCT 
GACAAAGCAA AACAAGAAAG AATAGAAGAT GCAATTAGAG AGAGAGCTAA AAAACAAAAA 
TCTGTCAGGG AATCAAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn8a ( XM_031895986.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751846.1 Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X2, mRNA.

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CloneID	OXa51382
Clone ID Related Accession (Same CDS sequence)	XM_031895986.1
Accession Version	XM_031895986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 8 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCGTATAT AACAGAGTTT GTAAACCTAG GCAATGTTTC AGCTCTGCGC 
ACTTTCAGAG TTCTGAGAGC TTTGAAAACT ATTTCTGTAA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGGTGA AAATAGATAA GGCAGCTACT GATGACAGCC CTACAACAGA AGTAGAAGTG 
AAAAGGAAGC CTTCAGGATC TCTTCGTGTG TCCATGGACC AGTTGACTGC ATCTTTTGGA 
AGGAAGAGAG CCAATAGTAT TCTGAGTGTT GTAACTCAGA CTTTAGTGGA AGAGCTGGAG 
GAATCACAAA GAAAGTGTCC CCCTTGTTGG TACAAATTCG CAGACACCTA TCTAATATGG 
ACTTGCTGTC CTTTGTGGAT TCGCATTAAA ACCATTGTGA ACATGATTGT AATGGATCCC 
TTTGTGGACT TGACCATCAC CATTTGTATT GTTCTTAACA CTTTATTTAT GGCTATGGAA 
CACTATCCAA TGACACCACA TTTTGAAAAT GTTTTGGTTG TGGGAAATCT GGTTTTCACT 
GGCATTTTTA CAGCAGAAAT GTTCTTGAAG TTGATAGCGA TGGATCCATA TTACTATTTT 
CAGGAGGGCT GGAACATATT TGATGGTTTT ATTGTTTCGC TCAGTTTAAT GGAATTAGGA 
CTTCAGGACG TTGAGGGGCT TTCTGTGCTG CGCTCCTTCC GCTTGCTGAG AGTCTTCAAA 
CTAGCTAAAT CATGGCCGAC ATTAAACATG CTGATAAAAA TCATTGGGAA TTCTGTTGGT 
GCCTTGGGGA ACTTGACCTT GGTGCTTGCC ATTATCGTTT TCATTTTTGC TGTAGTGGGC 
ATGCAGCTGT TTGGCAAAAG CTATAAGGAC TGTGTCTGCA AGATAAACCC AGAATGTGTA 
TTGCCACGCT GGCACATGAA TGATTTTTTT CATTCCTTCC TTATTGTTTT CCGGGTATTA 
TGTGGAGAGT GGATTGAAAC CATGTGGGAC TGCATGGAGG TTGCTGGACA AGCCATGTGT 
ATTATTGTCT TCATGATGGT GATGGTCATT GGTAATCTGG TGGTGCTGAA CCTTTTTCTG 
GCTTTACTGC TGAGCTCATT TAGCGCAGAC AACCTGGCAG CTACAGATGA TGATGGGGAG 
ATGAACAACC TGCAGATATC TGTCATTCGA ATCAAAAAAG GGATTGCTTG GATTAAGCTC 
AAAGCGCATG ATTTTATGCA AAAGCATTTT AAAAAGAAAG ACGCCGATGA AGTTAAACCT 
CTGGATGAAA TGTATGAGAA GAAGATGAAC TGTTTGGCAA ACAATACAGG TGCTGATATC 
CACAGAGATA TGGATTACCC AAAGAATGGA AATGGAACTA CCAGTGGCAT TGGGAGTAGT 
GTGGAAAAAT ACATGATAGA TGAGGATCAC ATGTCTTTCA TACATAACCC TAACCTCACC 
GTTAAAGTGC CCATTGCAGT TGGAGAGTCA GATTTTGAAA ATCTTAACAC GGAGGATTTC 
AGCAGTGCGT CTGATGAAGA AGGCAGCAAA GATAAACTGG ATGACACCAG TTCTTCAGAA 
GGAAGTACAA TTGATATAAA ACCAGAAGCT GAGGAAGTTC CTGTAGAGCA ACCAGAAGAG 
TATTTAGATC CGGATAACTG CTTTACTGAA GGCTGTGTGG CTCGTTTTAA GTGTTGCCAA 
GTCAGTGTGG AAGAAGGTTT GGGTAAAAGT TGGTGGACAC TGCGGAAAAC CTGTTTCCTC 
ATAGTGGAGC ACAACTGGTT TGAAACCTTC ATCATTTTCA TGATATTGTT AAGCAGTGGA 
GCGCTGGCCT TTGAGGATGT TTACATAGAA CAGCGGAAAA CCATTCGAAC GATCCTTGAA 
TATGCAGATA AAGTTTTCAC TTACATTTTC ATATTGGAAA TGTTGCTGAA ATGGTTGGCA 
TATGGATTTG TGAAGTTCTT CACTAATGCC TGGTGTTGGC TAGACTTCCT CATTGTTGAT 
GTCTCTTTAG TAAGCCTTAT AGCTAATGCC CTGGGCTACT CGGAACTAGG TGCCATTAAG 
TCCCTTAGGA CCTTAAGAGC ATTGAGACCC CTAAGAGCCT TGTCACGATT TGAAGGGATG 
AGGGTGGTAG TTAATGCCTT GGTTGGTGCC ATCCCTTCAA TTATGAATGT GTTGCTGGTC 
TGCCTTATAT TTTGGCTGAT TTTTAGTATC ATGGGTGTTA ACCTGTTTGC GGGCAAGTAC 
TACTACTGCT ATAACGAAAC GGCAGAAGGC ATGTTTGAAA TTGAAGACGT GGAAAATGAA 
ACACAATGTT TTGACCTTAT TGGAAATAAT TATACCGAAG TTAGATGGAA AAATGTGAAA 
ATCAACTTTG ACAATGTTGG AGCTGGATAT CTTGCATTGC TTCAAGTAGC TACTTTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAGTA GACTCCAGGA AGGTTCACCA GCAACCAAAA 
TATGAGGACA ACATTTATAT GTACATTTAT TTTGTAATCT TCATTATTTT TGGCTCTTTC 
TTCACTCTAA ATCTGTTTAT TGGTGTCATC ATTGATAACT TCAATCAACA AAAGAAAAAG 
TTTGGAGGTC AAGACATCTT CATGACAGAA GAACAAAAAA AGTATTATAA TGCTATGAAG 
AAACTGGGCT CTAAGAAACC ACAAAAACCC ATACCCAGGC CACTAAATAA ATTCCAAGGA 
GCCATTTTTG ATATAATCAC ACAACAAGCA TTTGATATTG TGATTATGAT TCTGATTTGT 
CTTAACATGG TGACAATGAT GGTAGAAACC GATGACCAGA GTGATTACAC AGATAATGTT 
CTCTACTGGA TCAATGTTGT TTTCATTGTA TTTTTTACCA CTGAGTGTGT GCTAAAGCTG 
TGTGCACTGC GGCATTATTA CTTTACCATC GGATGGAATA TTTTCGACTT TGTTGTTGTA 
ATCCTTTCAA TTGTTGGAAT GTTCCTGGCT GATTTGATCA CAAAATACTT TGTGTCACCC 
ACTTTGTTCA GAGTCATTCG TCTGGCTCGT ATTGGCCGCA TACTTCGCTT AATCAAGGGA 
GCCAAAGGGA TACGGACCCT GCTTTTTGCC TTAATGATGT CATTGCCGGC TTTGTTCAAC 
ATCGGCCTTC TTCTGTTTCT AGTGATGTTT ATATTTTCAA TATTTGGTAT GTCGAACTTC 
GCTTATGTGA AGAAGGAAGC AGGGATAGAT GACATGTTCA ACTTTGAAAC CTTTGGCAAT 
AGTATGATCT GCTTATTTCA GATCACTACA TCTGCTGGCT GGGATGGCTT GTTGCTGCCT 
ATCCTAAATC GTCCGCCAGA TTGTGATGCG CACAAAGAGA ATCCAGGTAG TCAAGTGAAA 
GGTGACTGCG GAAATCCTTC TGTTGGCATC TTTTTCTTTG TCAGCTACAT TATAATCTCT 
TTCCTGATTG TAGTGAACAT GTACATTGCT ATCATTCTGG AAAATTTCAG CGTGGCAACT 
GAAGAGAGTG CTGATCCTTT AAGTGAGGAT GACTTTGAAA CCTTTTATGA AATTTGGGAG 
AAATTTGATC CAGATGCAAC ACAGTTTATC CAGTTCAGCA AACTGCCAGA TTTCGCAGAT 
GCCTTGGAGC ATCCACTGCG AATCCCCAAG CCAAACACCA TTGAACTAAT TGCAATGGAT 
TTGCCGATGG TGAGTGGTGA CAGAATTCAT TGTCTGGATA TCCTATTTGC CTTCACCAAA 
CGGGTACTTG GAGAGAGCGG AGAGCTGGAT ATATTGCGCC AGCAGATGGA AGAGAGATTT 
GTGGCTTCAA ACCCTTCAAA AGTGTCTTAT GAGCCCATTA CCACCACACT CAGGCGAAAG 
CAAGAGGATG TATCTGCGGT TGTTATCCAG CAAGCTTACA GGCGCCATTT AGTCAAACGA 
GGGTTTATTT CTAGAAAGCG TTCAACTGGC AAAATGGAGA ATGGGGGAAC CAACATGGAT 
AAAAAAGAGG GAACGCCATC TACTGCATCA CTCCCATCAT ATGACAGTGT AACTAAACCT 
GACAAAGCAA AACAAGAAAG AATAGAAGAT GCAATTAGAG AGAGAGCTAA AAAACAAAAA 
TCTGTCAGGG AATCAAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751846.1
CDS	315..6278
Translation	MAAPLMAPPGPDSFKAFTRESLAAIEKRIAEEKNKKPPKQDSSHRDDDDENKPRPNSDLEAGKGVPFIYGDCPSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYITEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCVAWPVDMDQMYLANGSKGFDFDEYIMNDTNFFKVHGAIDPLLCGNGSDAGQCPEGYTCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAERKENEFKAMLEQMKRMQEEAQAAANATSAGTVTEDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKELSEGEEKGDSEKVYKSESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSRHNSKSSIFSFRGRFRDPVSENEFADDEHSTVEESEGRRDSLFIPVRGHDRRNSYNSGYSQGSRSSRILQNLRRNPKRNSTVDCNGVVSLIGGPGSNIPGGRLLPEVKIDKAATDDSPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWIRIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLQDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMNCLANNTGADIHRDMDYPKNGNGTTSGIGSSVEKYMIDEDHMSFIHNPNLTVKVPIAVGESDFENLNTEDFSSASDEEGSKDKLDDTSSSEGSTIDIKPEAEEVPVEQPEEYLDPDNCFTEGCVARFKCCQVSVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGESGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEDVSAVVIQQAYRRHLVKRGFISRKRSTGKMENGGTNMDKKEGTPSTASLPSYDSVTKPDKAKQERIEDAIRERAKKQKSVRESKC

Target ORF information:

RefSeq Version	XM_031895986.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895986.1

ORF Insert Sequence:

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCGTATAT AACAGAGTTT GTAAACCTAG GCAATGTTTC AGCTCTGCGC 
ACTTTCAGAG TTCTGAGAGC TTTGAAAACT ATTTCTGTAA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGGTGA AAATAGATAA GGCAGCTACT GATGACAGCC CTACAACAGA AGTAGAAGTG 
AAAAGGAAGC CTTCAGGATC TCTTCGTGTG TCCATGGACC AGTTGACTGC ATCTTTTGGA 
AGGAAGAGAG CCAATAGTAT TCTGAGTGTT GTAACTCAGA CTTTAGTGGA AGAGCTGGAG 
GAATCACAAA GAAAGTGTCC CCCTTGTTGG TACAAATTCG CAGACACCTA TCTAATATGG 
ACTTGCTGTC CTTTGTGGAT TCGCATTAAA ACCATTGTGA ACATGATTGT AATGGATCCC 
TTTGTGGACT TGACCATCAC CATTTGTATT GTTCTTAACA CTTTATTTAT GGCTATGGAA 
CACTATCCAA TGACACCACA TTTTGAAAAT GTTTTGGTTG TGGGAAATCT GGTTTTCACT 
GGCATTTTTA CAGCAGAAAT GTTCTTGAAG TTGATAGCGA TGGATCCATA TTACTATTTT 
CAGGAGGGCT GGAACATATT TGATGGTTTT ATTGTTTCGC TCAGTTTAAT GGAATTAGGA 
CTTCAGGACG TTGAGGGGCT TTCTGTGCTG CGCTCCTTCC GCTTGCTGAG AGTCTTCAAA 
CTAGCTAAAT CATGGCCGAC ATTAAACATG CTGATAAAAA TCATTGGGAA TTCTGTTGGT 
GCCTTGGGGA ACTTGACCTT GGTGCTTGCC ATTATCGTTT TCATTTTTGC TGTAGTGGGC 
ATGCAGCTGT TTGGCAAAAG CTATAAGGAC TGTGTCTGCA AGATAAACCC AGAATGTGTA 
TTGCCACGCT GGCACATGAA TGATTTTTTT CATTCCTTCC TTATTGTTTT CCGGGTATTA 
TGTGGAGAGT GGATTGAAAC CATGTGGGAC TGCATGGAGG TTGCTGGACA AGCCATGTGT 
ATTATTGTCT TCATGATGGT GATGGTCATT GGTAATCTGG TGGTGCTGAA CCTTTTTCTG 
GCTTTACTGC TGAGCTCATT TAGCGCAGAC AACCTGGCAG CTACAGATGA TGATGGGGAG 
ATGAACAACC TGCAGATATC TGTCATTCGA ATCAAAAAAG GGATTGCTTG GATTAAGCTC 
AAAGCGCATG ATTTTATGCA AAAGCATTTT AAAAAGAAAG ACGCCGATGA AGTTAAACCT 
CTGGATGAAA TGTATGAGAA GAAGATGAAC TGTTTGGCAA ACAATACAGG TGCTGATATC 
CACAGAGATA TGGATTACCC AAAGAATGGA AATGGAACTA CCAGTGGCAT TGGGAGTAGT 
GTGGAAAAAT ACATGATAGA TGAGGATCAC ATGTCTTTCA TACATAACCC TAACCTCACC 
GTTAAAGTGC CCATTGCAGT TGGAGAGTCA GATTTTGAAA ATCTTAACAC GGAGGATTTC 
AGCAGTGCGT CTGATGAAGA AGGCAGCAAA GATAAACTGG ATGACACCAG TTCTTCAGAA 
GGAAGTACAA TTGATATAAA ACCAGAAGCT GAGGAAGTTC CTGTAGAGCA ACCAGAAGAG 
TATTTAGATC CGGATAACTG CTTTACTGAA GGCTGTGTGG CTCGTTTTAA GTGTTGCCAA 
GTCAGTGTGG AAGAAGGTTT GGGTAAAAGT TGGTGGACAC TGCGGAAAAC CTGTTTCCTC 
ATAGTGGAGC ACAACTGGTT TGAAACCTTC ATCATTTTCA TGATATTGTT AAGCAGTGGA 
GCGCTGGCCT TTGAGGATGT TTACATAGAA CAGCGGAAAA CCATTCGAAC GATCCTTGAA 
TATGCAGATA AAGTTTTCAC TTACATTTTC ATATTGGAAA TGTTGCTGAA ATGGTTGGCA 
TATGGATTTG TGAAGTTCTT CACTAATGCC TGGTGTTGGC TAGACTTCCT CATTGTTGAT 
GTCTCTTTAG TAAGCCTTAT AGCTAATGCC CTGGGCTACT CGGAACTAGG TGCCATTAAG 
TCCCTTAGGA CCTTAAGAGC ATTGAGACCC CTAAGAGCCT TGTCACGATT TGAAGGGATG 
AGGGTGGTAG TTAATGCCTT GGTTGGTGCC ATCCCTTCAA TTATGAATGT GTTGCTGGTC 
TGCCTTATAT TTTGGCTGAT TTTTAGTATC ATGGGTGTTA ACCTGTTTGC GGGCAAGTAC 
TACTACTGCT ATAACGAAAC GGCAGAAGGC ATGTTTGAAA TTGAAGACGT GGAAAATGAA 
ACACAATGTT TTGACCTTAT TGGAAATAAT TATACCGAAG TTAGATGGAA AAATGTGAAA 
ATCAACTTTG ACAATGTTGG AGCTGGATAT CTTGCATTGC TTCAAGTAGC TACTTTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAGTA GACTCCAGGA AGGTTCACCA GCAACCAAAA 
TATGAGGACA ACATTTATAT GTACATTTAT TTTGTAATCT TCATTATTTT TGGCTCTTTC 
TTCACTCTAA ATCTGTTTAT TGGTGTCATC ATTGATAACT TCAATCAACA AAAGAAAAAG 
TTTGGAGGTC AAGACATCTT CATGACAGAA GAACAAAAAA AGTATTATAA TGCTATGAAG 
AAACTGGGCT CTAAGAAACC ACAAAAACCC ATACCCAGGC CACTAAATAA ATTCCAAGGA 
GCCATTTTTG ATATAATCAC ACAACAAGCA TTTGATATTG TGATTATGAT TCTGATTTGT 
CTTAACATGG TGACAATGAT GGTAGAAACC GATGACCAGA GTGATTACAC AGATAATGTT 
CTCTACTGGA TCAATGTTGT TTTCATTGTA TTTTTTACCA CTGAGTGTGT GCTAAAGCTG 
TGTGCACTGC GGCATTATTA CTTTACCATC GGATGGAATA TTTTCGACTT TGTTGTTGTA 
ATCCTTTCAA TTGTTGGAAT GTTCCTGGCT GATTTGATCA CAAAATACTT TGTGTCACCC 
ACTTTGTTCA GAGTCATTCG TCTGGCTCGT ATTGGCCGCA TACTTCGCTT AATCAAGGGA 
GCCAAAGGGA TACGGACCCT GCTTTTTGCC TTAATGATGT CATTGCCGGC TTTGTTCAAC 
ATCGGCCTTC TTCTGTTTCT AGTGATGTTT ATATTTTCAA TATTTGGTAT GTCGAACTTC 
GCTTATGTGA AGAAGGAAGC AGGGATAGAT GACATGTTCA ACTTTGAAAC CTTTGGCAAT 
AGTATGATCT GCTTATTTCA GATCACTACA TCTGCTGGCT GGGATGGCTT GTTGCTGCCT 
ATCCTAAATC GTCCGCCAGA TTGTGATGCG CACAAAGAGA ATCCAGGTAG TCAAGTGAAA 
GGTGACTGCG GAAATCCTTC TGTTGGCATC TTTTTCTTTG TCAGCTACAT TATAATCTCT 
TTCCTGATTG TAGTGAACAT GTACATTGCT ATCATTCTGG AAAATTTCAG CGTGGCAACT 
GAAGAGAGTG CTGATCCTTT AAGTGAGGAT GACTTTGAAA CCTTTTATGA AATTTGGGAG 
AAATTTGATC CAGATGCAAC ACAGTTTATC CAGTTCAGCA AACTGCCAGA TTTCGCAGAT 
GCCTTGGAGC ATCCACTGCG AATCCCCAAG CCAAACACCA TTGAACTAAT TGCAATGGAT 
TTGCCGATGG TGAGTGGTGA CAGAATTCAT TGTCTGGATA TCCTATTTGC CTTCACCAAA 
CGGGTACTTG GAGAGAGCGG AGAGCTGGAT ATATTGCGCC AGCAGATGGA AGAGAGATTT 
GTGGCTTCAA ACCCTTCAAA AGTGTCTTAT GAGCCCATTA CCACCACACT CAGGCGAAAG 
CAAGAGGATG TATCTGCGGT TGTTATCCAG CAAGCTTACA GGCGCCATTT AGTCAAACGA 
GGGTTTATTT CTAGAAAGCG TTCAACTGGC AAAATGGAGA ATGGGGGAAC CAACATGGAT 
AAAAAAGAGG GAACGCCATC TACTGCATCA CTCCCATCAT ATGACAGTGT AACTAAACCT 
GACAAAGCAA AACAAGAAAG AATAGAAGAT GCAATTAGAG AGAGAGCTAA AAAACAAAAA 
TCTGTCAGGG AATCAAAGTG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn8a ( XM_031895987.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751847.1 Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X3, mRNA.

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CloneID	OXa51383
Clone ID Related Accession (Same CDS sequence)	XM_031895987.1
Accession Version	XM_031895987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 8 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCGTATAT AACAGAGTTT GTAAACCTAG GCAATGTTTC AGCTCTGCGC 
ACTTTCAGAG TTCTGAGAGC TTTGAAAACT ATTTCTGTAA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGCCTA CAACAGAAGT AGAAGTGAAA AGGAAGCCTT CAGGATCTCT TCGTGTGTCC 
ATGGACCAGT TGACTGCATC TTTTGGAAGG AAGAGAGCCA ATAGTATTCT GAGTGTTGTA 
ACTCAGACTT TAGTGGAAGA GCTGGAGGAA TCACAAAGAA AGTGTCCCCC TTGTTGGTAC 
AAATTCGCAG ACACCTATCT AATATGGACT TGCTGTCCTT TGTGGATTCG CATTAAAACC 
ATTGTGAACA TGATTGTAAT GGATCCCTTT GTGGACTTGA CCATCACCAT TTGTATTGTT 
CTTAACACTT TATTTATGGC TATGGAACAC TATCCAATGA CACCACATTT TGAAAATGTT 
TTGGTTGTGG GAAATCTGGT TTTCACTGGC ATTTTTACAG CAGAAATGTT CTTGAAGTTG 
ATAGCGATGG ATCCATATTA CTATTTTCAG GAGGGCTGGA ACATATTTGA TGGTTTTATT 
GTTTCGCTCA GTTTAATGGA ATTAGGACTT CAGGACGTTG AGGGGCTTTC TGTGCTGCGC 
TCCTTCCGCT TGCTGAGAGT CTTCAAACTA GCTAAATCAT GGCCGACATT AAACATGCTG 
ATAAAAATCA TTGGGAATTC TGTTGGTGCC TTGGGGAACT TGACCTTGGT GCTTGCCATT 
ATCGTTTTCA TTTTTGCTGT AGTGGGCATG CAGCTGTTTG GCAAAAGCTA TAAGGACTGT 
GTCTGCAAGA TAAACCCAGA ATGTGTATTG CCACGCTGGC ACATGAATGA TTTTTTTCAT 
TCCTTCCTTA TTGTTTTCCG GGTATTATGT GGAGAGTGGA TTGAAACCAT GTGGGACTGC 
ATGGAGGTTG CTGGACAAGC CATGTGTATT ATTGTCTTCA TGATGGTGAT GGTCATTGGT 
AATCTGGTGG TGCTGAACCT TTTTCTGGCT TTACTGCTGA GCTCATTTAG CGCAGACAAC 
CTGGCAGCTA CAGATGATGA TGGGGAGATG AACAACCTGC AGATATCTGT CATTCGAATC 
AAAAAAGGGA TTGCTTGGAT TAAGCTCAAA GCGCATGATT TTATGCAAAA GCATTTTAAA 
AAGAAAGACG CCGATGAAGT TAAACCTCTG GATGAAATGT ATGAGAAGAA GATGAACTGT 
TTGGCAAACA ATACAGGTGC TGATATCCAC AGAGATATGG ATTACCCAAA GAATGGAAAT 
GGAACTACCA GTGGCATTGG GAGTAGTGTG GAAAAATACA TGATAGATGA GGATCACATG 
TCTTTCATAC ATAACCCTAA CCTCACCGTT AAAGTGCCCA TTGCAGTTGG AGAGTCAGAT 
TTTGAAAATC TTAACACGGA GGATTTCAGC AGTGCGTCTG ATGAAGAAGG CAGCAAAGAT 
AAACTGGATG ACACCAGTTC TTCAGAAGGA AGTACAATTG ATATAAAACC AGAAGCTGAG 
GAAGTTCCTG TAGAGCAACC AGAAGAGTAT TTAGATCCGG ATAACTGCTT TACTGAAGGC 
TGTGTGGCTC GTTTTAAGTG TTGCCAAGTC AGTGTGGAAG AAGGTTTGGG TAAAAGTTGG 
TGGACACTGC GGAAAACCTG TTTCCTCATA GTGGAGCACA ACTGGTTTGA AACCTTCATC 
ATTTTCATGA TATTGTTAAG CAGTGGAGCG CTGGCCTTTG AGGATGTTTA CATAGAACAG 
CGGAAAACCA TTCGAACGAT CCTTGAATAT GCAGATAAAG TTTTCACTTA CATTTTCATA 
TTGGAAATGT TGCTGAAATG GTTGGCATAT GGATTTGTGA AGTTCTTCAC TAATGCCTGG 
TGTTGGCTAG ACTTCCTCAT TGTTGATGTC TCTTTAGTAA GCCTTATAGC TAATGCCCTG 
GGCTACTCGG AACTAGGTGC CATTAAGTCC CTTAGGACCT TAAGAGCATT GAGACCCCTA 
AGAGCCTTGT CACGATTTGA AGGGATGAGG GTGGTAGTTA ATGCCTTGGT TGGTGCCATC 
CCTTCAATTA TGAATGTGTT GCTGGTCTGC CTTATATTTT GGCTGATTTT TAGTATCATG 
GGTGTTAACC TGTTTGCGGG CAAGTACTAC TACTGCTATA ACGAAACGGC AGAAGGCATG 
TTTGAAATTG AAGACGTGGA AAATGAAACA CAATGTTTTG ACCTTATTGG AAATAATTAT 
ACCGAAGTTA GATGGAAAAA TGTGAAAATC AACTTTGACA ATGTTGGAGC TGGATATCTT 
GCATTGCTTC AAGTAGCTAC TTTTAAAGGC TGGATGGATA TTATGTATGC AGCAGTAGAC 
TCCAGGAAGG TTCACCAGCA ACCAAAATAT GAGGACAACA TTTATATGTA CATTTATTTT 
GTAATCTTCA TTATTTTTGG CTCTTTCTTC ACTCTAAATC TGTTTATTGG TGTCATCATT 
GATAACTTCA ATCAACAAAA GAAAAAGTTT GGAGGTCAAG ACATCTTCAT GACAGAAGAA 
CAAAAAAAGT ATTATAATGC TATGAAGAAA CTGGGCTCTA AGAAACCACA AAAACCCATA 
CCCAGGCCAC TAAATAAATT CCAAGGAGCC ATTTTTGATA TAATCACACA ACAAGCATTT 
GATATTGTGA TTATGATTCT GATTTGTCTT AACATGGTGA CAATGATGGT AGAAACCGAT 
GACCAGAGTG ATTACACAGA TAATGTTCTC TACTGGATCA ATGTTGTTTT CATTGTATTT 
TTTACCACTG AGTGTGTGCT AAAGCTGTGT GCACTGCGGC ATTATTACTT TACCATCGGA 
TGGAATATTT TCGACTTTGT TGTTGTAATC CTTTCAATTG TTGGAATGTT CCTGGCTGAT 
TTGATCACAA AATACTTTGT GTCACCCACT TTGTTCAGAG TCATTCGTCT GGCTCGTATT 
GGCCGCATAC TTCGCTTAAT CAAGGGAGCC AAAGGGATAC GGACCCTGCT TTTTGCCTTA 
ATGATGTCAT TGCCGGCTTT GTTCAACATC GGCCTTCTTC TGTTTCTAGT GATGTTTATA 
TTTTCAATAT TTGGTATGTC GAACTTCGCT TATGTGAAGA AGGAAGCAGG GATAGATGAC 
ATGTTCAACT TTGAAACCTT TGGCAATAGT ATGATCTGCT TATTTCAGAT CACTACATCT 
GCTGGCTGGG ATGGCTTGTT GCTGCCTATC CTAAATCGTC CGCCAGATTG TGATGCGCAC 
AAAGAGAATC CAGGTAGTCA AGTGAAAGGT GACTGCGGAA ATCCTTCTGT TGGCATCTTT 
TTCTTTGTCA GCTACATTAT AATCTCTTTC CTGATTGTAG TGAACATGTA CATTGCTATC 
ATTCTGGAAA ATTTCAGCGT GGCAACTGAA GAGAGTGCTG ATCCTTTAAG TGAGGATGAC 
TTTGAAACCT TTTATGAAAT TTGGGAGAAA TTTGATCCAG ATGCAACACA GTTTATCCAG 
TTCAGCAAAC TGCCAGATTT CGCAGATGCC TTGGAGCATC CACTGCGAAT CCCCAAGCCA 
AACACCATTG AACTAATTGC AATGGATTTG CCGATGGTGA GTGGTGACAG AATTCATTGT 
CTGGATATCC TATTTGCCTT CACCAAACGG GTACTTGGAG AGAGCGGAGA GCTGGATATA 
TTGCGCCAGC AGATGGAAGA GAGATTTGTG GCTTCAAACC CTTCAAAAGT GTCTTATGAG 
CCCATTACCA CCACACTCAG GCGAAAGCAA GAGGATGTAT CTGCGGTTGT TATCCAGCAA 
GCTTACAGGC GCCATTTAGT CAAACGAGGG TTTATTTCTA GAAAGCGTTC AACTGGCAAA 
ATGGAGAATG GGGGAACCAA CATGGATAAA AAAGAGGGAA CGCCATCTAC TGCATCACTC 
CCATCATATG ACAGTGTAAC TAAACCTGAC AAAGCAAAAC AAGAAAGAAT AGAAGATGCA 
ATTAGAGAGA GAGCTAAAAA ACAAAAATCT GTCAGGGAAT CAAAGTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751847.1
CDS	315..6245
Translation	MAAPLMAPPGPDSFKAFTRESLAAIEKRIAEEKNKKPPKQDSSHRDDDDENKPRPNSDLEAGKGVPFIYGDCPSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHSLFSMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYITEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCVAWPVDMDQMYLANGSKGFDFDEYIMNDTNFFKVHGAIDPLLCGNGSDAGQCPEGYTCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAERKENEFKAMLEQMKRMQEEAQAAANATSAGTVTEDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKELSEGEEKGDSEKVYKSESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSRHNSKSSIFSFRGRFRDPVSENEFADDEHSTVEESEGRRDSLFIPVRGHDRRNSYNSGYSQGSRSSRILQNLRRNPKRNSTVDCNGVVSLIGGPGSNIPGGRLLPEPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWIRIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLQDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMNCLANNTGADIHRDMDYPKNGNGTTSGIGSSVEKYMIDEDHMSFIHNPNLTVKVPIAVGESDFENLNTEDFSSASDEEGSKDKLDDTSSSEGSTIDIKPEAEEVPVEQPEEYLDPDNCFTEGCVARFKCCQVSVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGESGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEDVSAVVIQQAYRRHLVKRGFISRKRSTGKMENGGTNMDKKEGTPSTASLPSYDSVTKPDKAKQERIEDAIRERAKKQKSVRESKC

Target ORF information:

RefSeq Version	XM_031895987.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type VIII alpha subunit (scn8a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895987.1

ORF Insert Sequence:

ATGGCAGCTC CGCTTATGGC ACCACCTGGC CCTGATAGCT TCAAAGCTTT TACTCGTGAA 
TCTTTGGCTG CCATTGAAAA GCGCATAGCC GAGGAGAAGA ATAAAAAGCC ACCAAAGCAA 
GACAGTAGCC ACAGAGACGA TGATGATGAG AATAAACCCC GTCCAAACAG TGATCTGGAA 
GCGGGCAAAG GCGTGCCGTT TATTTATGGC GATTGTCCTT CACGCCTGAT TGCTGAGCCA 
ATCGAAGACT TAGACCCTTT TTATTTACAC ACAAAAACCT TTATAGTACT AAACAGAGGA 
AAAACAATCT TCAGATTTAG TGCCGCGCCT GCCTTGTATA TTTTAAGTCC TTTTAATCCA 
CTAAGAAAAT TTGCTATTAA AATTTTGATA CATTCATTAT TTAGCATGAT CATTATGTGC 
ACTATTTTGA CCAACTGCGT ATTCATGACT TTTAGTAATC CTCCTGAATG GTCCAAACAA 
GTGGAATATA CGTTCACTGG CATTTATACA TTTGAATCAC TTGTGAAAAT CATTGCGAGA 
GGTTTTTGCA TAGACGGCTT TACATTTTTG CGGGACCCAT GGAACTGGCT AGATTTCAGC 
GTGATAATGA TGGCGTATAT AACAGAGTTT GTAAACCTAG GCAATGTTTC AGCTCTGCGC 
ACTTTCAGAG TTCTGAGAGC TTTGAAAACT ATTTCTGTAA TTCCAGGCCT GAAGACCATT 
GTAGGTGCCC TAATTCAGTC TGTGAAGAAG CTGTCAGATG TAATGATTTT GACAGTGTTT 
TGCCTCAGTG TCTTTGCTTT AATCGGTCTA CAGCTTTTCA TGGGCCATCT AAGGCGCAAA 
TGTGTTGCTT GGCCTGTGGA CATGGACCAG ATGTACTTGG CCAATGGCAG CAAAGGATTC 
GATTTTGACG AATACATCAT GAATGACACA AACTTTTTTA AAGTCCATGG AGCAATCGAT 
CCGTTACTCT GCGGTAATGG CTCAGACGCT GGGCAATGCC CAGAGGGATA CACATGTATG 
AAAGCAGGGA GGAACCCAAA TTATGGCTAT ACTAGCTTTG ATACATTTAG CTGGGCCTTC 
TTGGCTTTAT TTCGCCTTAT GACTCAGGAT TTTTGGGAAA ACTTGTACCA GTTAACATTG 
CGTGCTTCTG GAAAAACATA TATGATCTTC TTTGTCTTGG TTATCTTTGT GGGGTCATTT 
TACCTGGTGA ATTTAATTCT GGCTGTAGTG GCCATGGCTT ATGAAGAACA AAACCAAGCC 
ACCTTGGAGG AAGCAGAGCG GAAGGAGAAT GAATTTAAGG CAATGCTGGA ACAGATGAAA 
AGGATGCAGG AAGAAGCTCA GGCTGCAGCA AATGCTACAT CTGCTGGCAC AGTGACTGAG 
GATGCTGTGG AAGATGAAGA TGGAGAGGCC CGGATGTCTC ACACATCTTC TGAGTTCTCC 
AAACCAAGTT CAAAGAGTGC CAAGGAAAGG AGAAATCGTA GAAAGAAGCG GAAACAGAAA 
GAATTGTCTG AAGGGGAAGA GAAAGGAGAT AGTGAAAAGG TGTACAAGTC TGAGTCTGAA 
GATGGGGTAA AAAGAAGAGG CTTCTGGTAC CCTGATAATA GACTGGGAAG AAGGTTTTCA 
ATCATGAACC AGTCTCTTCT GAGCATACCA GGGTCTCCAT ACCTATCAAG ACACAACAGC 
AAGAGCAGTA TATTCAGTTT TAGAGGACGT TTCAGGGATC CAGTCTCTGA AAATGAGTTT 
GCAGATGACG AACACAGCAC AGTAGAAGAG AGCGAAGGCC GACGCGATTC CCTTTTCATC 
CCAGTCCGTG GACATGACAG AAGGAACAGC TACAATAGTG GTTACAGTCA GGGCAGTCGA 
TCGTCTCGCA TCCTCCAGAA CCTGCGGAGA AATCCGAAAA GGAATAGCAC GGTGGATTGT 
AACGGCGTGG TCTCTCTGAT TGGTGGTCCA GGCTCCAATA TCCCTGGTGG ACGTCTTCTG 
CCTGAGCCTA CAACAGAAGT AGAAGTGAAA AGGAAGCCTT CAGGATCTCT TCGTGTGTCC 
ATGGACCAGT TGACTGCATC TTTTGGAAGG AAGAGAGCCA ATAGTATTCT GAGTGTTGTA 
ACTCAGACTT TAGTGGAAGA GCTGGAGGAA TCACAAAGAA AGTGTCCCCC TTGTTGGTAC 
AAATTCGCAG ACACCTATCT AATATGGACT TGCTGTCCTT TGTGGATTCG CATTAAAACC 
ATTGTGAACA TGATTGTAAT GGATCCCTTT GTGGACTTGA CCATCACCAT TTGTATTGTT 
CTTAACACTT TATTTATGGC TATGGAACAC TATCCAATGA CACCACATTT TGAAAATGTT 
TTGGTTGTGG GAAATCTGGT TTTCACTGGC ATTTTTACAG CAGAAATGTT CTTGAAGTTG 
ATAGCGATGG ATCCATATTA CTATTTTCAG GAGGGCTGGA ACATATTTGA TGGTTTTATT 
GTTTCGCTCA GTTTAATGGA ATTAGGACTT CAGGACGTTG AGGGGCTTTC TGTGCTGCGC 
TCCTTCCGCT TGCTGAGAGT CTTCAAACTA GCTAAATCAT GGCCGACATT AAACATGCTG 
ATAAAAATCA TTGGGAATTC TGTTGGTGCC TTGGGGAACT TGACCTTGGT GCTTGCCATT 
ATCGTTTTCA TTTTTGCTGT AGTGGGCATG CAGCTGTTTG GCAAAAGCTA TAAGGACTGT 
GTCTGCAAGA TAAACCCAGA ATGTGTATTG CCACGCTGGC ACATGAATGA TTTTTTTCAT 
TCCTTCCTTA TTGTTTTCCG GGTATTATGT GGAGAGTGGA TTGAAACCAT GTGGGACTGC 
ATGGAGGTTG CTGGACAAGC CATGTGTATT ATTGTCTTCA TGATGGTGAT GGTCATTGGT 
AATCTGGTGG TGCTGAACCT TTTTCTGGCT TTACTGCTGA GCTCATTTAG CGCAGACAAC 
CTGGCAGCTA CAGATGATGA TGGGGAGATG AACAACCTGC AGATATCTGT CATTCGAATC 
AAAAAAGGGA TTGCTTGGAT TAAGCTCAAA GCGCATGATT TTATGCAAAA GCATTTTAAA 
AAGAAAGACG CCGATGAAGT TAAACCTCTG GATGAAATGT ATGAGAAGAA GATGAACTGT 
TTGGCAAACA ATACAGGTGC TGATATCCAC AGAGATATGG ATTACCCAAA GAATGGAAAT 
GGAACTACCA GTGGCATTGG GAGTAGTGTG GAAAAATACA TGATAGATGA GGATCACATG 
TCTTTCATAC ATAACCCTAA CCTCACCGTT AAAGTGCCCA TTGCAGTTGG AGAGTCAGAT 
TTTGAAAATC TTAACACGGA GGATTTCAGC AGTGCGTCTG ATGAAGAAGG CAGCAAAGAT 
AAACTGGATG ACACCAGTTC TTCAGAAGGA AGTACAATTG ATATAAAACC AGAAGCTGAG 
GAAGTTCCTG TAGAGCAACC AGAAGAGTAT TTAGATCCGG ATAACTGCTT TACTGAAGGC 
TGTGTGGCTC GTTTTAAGTG TTGCCAAGTC AGTGTGGAAG AAGGTTTGGG TAAAAGTTGG 
TGGACACTGC GGAAAACCTG TTTCCTCATA GTGGAGCACA ACTGGTTTGA AACCTTCATC 
ATTTTCATGA TATTGTTAAG CAGTGGAGCG CTGGCCTTTG AGGATGTTTA CATAGAACAG 
CGGAAAACCA TTCGAACGAT CCTTGAATAT GCAGATAAAG TTTTCACTTA CATTTTCATA 
TTGGAAATGT TGCTGAAATG GTTGGCATAT GGATTTGTGA AGTTCTTCAC TAATGCCTGG 
TGTTGGCTAG ACTTCCTCAT TGTTGATGTC TCTTTAGTAA GCCTTATAGC TAATGCCCTG 
GGCTACTCGG AACTAGGTGC CATTAAGTCC CTTAGGACCT TAAGAGCATT GAGACCCCTA 
AGAGCCTTGT CACGATTTGA AGGGATGAGG GTGGTAGTTA ATGCCTTGGT TGGTGCCATC 
CCTTCAATTA TGAATGTGTT GCTGGTCTGC CTTATATTTT GGCTGATTTT TAGTATCATG 
GGTGTTAACC TGTTTGCGGG CAAGTACTAC TACTGCTATA ACGAAACGGC AGAAGGCATG 
TTTGAAATTG AAGACGTGGA AAATGAAACA CAATGTTTTG ACCTTATTGG AAATAATTAT 
ACCGAAGTTA GATGGAAAAA TGTGAAAATC AACTTTGACA ATGTTGGAGC TGGATATCTT 
GCATTGCTTC AAGTAGCTAC TTTTAAAGGC TGGATGGATA TTATGTATGC AGCAGTAGAC 
TCCAGGAAGG TTCACCAGCA ACCAAAATAT GAGGACAACA TTTATATGTA CATTTATTTT 
GTAATCTTCA TTATTTTTGG CTCTTTCTTC ACTCTAAATC TGTTTATTGG TGTCATCATT 
GATAACTTCA ATCAACAAAA GAAAAAGTTT GGAGGTCAAG ACATCTTCAT GACAGAAGAA 
CAAAAAAAGT ATTATAATGC TATGAAGAAA CTGGGCTCTA AGAAACCACA AAAACCCATA 
CCCAGGCCAC TAAATAAATT CCAAGGAGCC ATTTTTGATA TAATCACACA ACAAGCATTT 
GATATTGTGA TTATGATTCT GATTTGTCTT AACATGGTGA CAATGATGGT AGAAACCGAT 
GACCAGAGTG ATTACACAGA TAATGTTCTC TACTGGATCA ATGTTGTTTT CATTGTATTT 
TTTACCACTG AGTGTGTGCT AAAGCTGTGT GCACTGCGGC ATTATTACTT TACCATCGGA 
TGGAATATTT TCGACTTTGT TGTTGTAATC CTTTCAATTG TTGGAATGTT CCTGGCTGAT 
TTGATCACAA AATACTTTGT GTCACCCACT TTGTTCAGAG TCATTCGTCT GGCTCGTATT 
GGCCGCATAC TTCGCTTAAT CAAGGGAGCC AAAGGGATAC GGACCCTGCT TTTTGCCTTA 
ATGATGTCAT TGCCGGCTTT GTTCAACATC GGCCTTCTTC TGTTTCTAGT GATGTTTATA 
TTTTCAATAT TTGGTATGTC GAACTTCGCT TATGTGAAGA AGGAAGCAGG GATAGATGAC 
ATGTTCAACT TTGAAACCTT TGGCAATAGT ATGATCTGCT TATTTCAGAT CACTACATCT 
GCTGGCTGGG ATGGCTTGTT GCTGCCTATC CTAAATCGTC CGCCAGATTG TGATGCGCAC 
AAAGAGAATC CAGGTAGTCA AGTGAAAGGT GACTGCGGAA ATCCTTCTGT TGGCATCTTT 
TTCTTTGTCA GCTACATTAT AATCTCTTTC CTGATTGTAG TGAACATGTA CATTGCTATC 
ATTCTGGAAA ATTTCAGCGT GGCAACTGAA GAGAGTGCTG ATCCTTTAAG TGAGGATGAC 
TTTGAAACCT TTTATGAAAT TTGGGAGAAA TTTGATCCAG ATGCAACACA GTTTATCCAG 
TTCAGCAAAC TGCCAGATTT CGCAGATGCC TTGGAGCATC CACTGCGAAT CCCCAAGCCA 
AACACCATTG AACTAATTGC AATGGATTTG CCGATGGTGA GTGGTGACAG AATTCATTGT 
CTGGATATCC TATTTGCCTT CACCAAACGG GTACTTGGAG AGAGCGGAGA GCTGGATATA 
TTGCGCCAGC AGATGGAAGA GAGATTTGTG GCTTCAAACC CTTCAAAAGT GTCTTATGAG 
CCCATTACCA CCACACTCAG GCGAAAGCAA GAGGATGTAT CTGCGGTTGT TATCCAGCAA 
GCTTACAGGC GCCATTTAGT CAAACGAGGG TTTATTTCTA GAAAGCGTTC AACTGGCAAA 
ATGGAGAATG GGGGAACCAA CATGGATAAA AAAGAGGGAA CGCCATCTAC TGCATCACTC 
CCATCATATG ACAGTGTAAC TAAACCTGAC AAAGCAAAAC AAGAAAGAAT AGAAGATGCA 
ATTAGAGAGA GAGCTAAAAA ACAAAAATCT GTCAGGGAAT CAAAGTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expansion of voltage-dependent Na+ channel gene family in early tetrapods coincided with the emergence of terrestriality and increased brain complexity.
Molecular biology and evolution28(4)1415-24(2011 Apr)
Zakon HH,Jost MC,Lu Y

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scn8a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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