cyp2c37 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cyp2c37 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cyp2c37 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa27341 XM_012954680.1
Latest version!
Xenopus tropicalis cytochrome P450, family 2. subfamily c, polypeptide 37 (cyp2c37), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa27341 XM_031904481.1
Latest version!
Xenopus tropicalis cytochrome P450, family 2. subfamily c, polypeptide 37 (cyp2c37), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa27341
    Clone ID Related Accession (Same CDS sequence) XM_012954680.1 , XM_031904481.1
    Accession Version XM_012954680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cytochrome P450 2C3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683775.1) and transcript sequence (BC089736.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 161 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGATTT TTTTATGTTC TCGGTTTAAT GAGTCCCCGG AGCCGGTGCC GCACGTTGTA 
    TCAACAACAC AAATACCATC GGCTCTTTAC TGGGGCAAAG TCCAACCTCA TTCCTATATA
    AACTGGGTAA GAGCAGCAGC GATCTATAGC CTGTCTATTA CCCCCACAGG CAGTGACAGA
    GCCAACGCCA GCCGGGGTAT CAGGACCATG GGGGTTGTTA CCATCTTGTC CCTCGCTCTC
    TCCTTCATCC TCACTCTCCT CTTCCTCGTT TCCTATTGGA GGCAGCAGAA GAAATCTATC
    ACCCTCCCTC CCGGTCCTGC CCCTTTGCCC CTTCTGGGGA ACCTGCGCTA CACCCTACAT
    AAAAGCCACT ACAAATTCTT CCCAGAGCTC AGCAAGCGGT ACGGCCCAGT GTTCACCATC
    TGGCAGATGA CGGATCCGGT GGTGGTTCTG TGCGGGTACG AGATGGTGAG AGACGCCCTG
    ATGAATCACG CAGAGCAGTT TAGCGGTCGC CCCTTTTCCC CGGTGATCGA CCTTTACTCC
    AAGGGATACA GCTTCCCGAG CCTTCAGGGC GAGCGCTGGC GCCAACTCCG ACGCTTCACT
    CTCTCCTCCC TGCGGAACTT TGGGATGGGG AAGAAATCCA TGGAGGAACT GGTACTGGAA
    GAGGCACAAC ATCTCAACGC AGCGGTGTCT GAAACAGGAG GGAAGCCGTT TAACCCGGTT
    CACCTAACGG GCTGCGCCGT AGCCAATATA ACCAGCCGGG CGCTGCTGGG GGAGCAGTTC
    CAGTACCAGG ACCAGAAGTT ACGGGACCTT CTCCTAACAA CACGGAGATT CATCAGCAAC
    ACCCACTCCT TCCTACACCA GCTCAGTAAC ATGTTCCCCG TGCTCCTGTA TGTTCCGGCC
    TTCAGGCAGA AGATTTTCAG AGAAAGTTCC GAGCTGCTCG CATTTGTCAC GGAATATATT
    GAGCAGCACA AACAGACACT GGATCCGAAT TCTCCCAGGG ACTTTATTGA CTACTTCTTA
    TTGAAGATCA GAGAGGAGAA GATGGCGGCA CAAAGCAATT TCTGTGAGAC CAGCCTGCTA
    ATGACCATTA TAGCGCTACT CGCTGCCGGC ACCGAGACCA CCTCATCCAC CCTAGCGTTC
    TGCCTGGCTT TCATATCTAA CTACCCCGAC GCCCAGGCCA AGATACAGAG AGAGATGGAC
    GAGGTTGTGG GCCCCCAGCG CCCCCCAGAG ACGGGGGACC GAGCCCGCCT GCCCTACACC
    AACGCCGTTA TCCACGAGAT GCAGCGGCTG CTCGACTTGG CGCCCATCGC CCATTTTCAC
    GCCGTCACCG AGGACACGGA GTTCCAGGGA TTTACTATCC CCAAGGGAAC CACCGTTATC
    CCCTTCATCT CCTCGGTGCT GTTCGACCCG ACCCAGTGGG AAACCCCTGA GGAGTTCAAC
    CCGGGGCATT TTCTGGATGG TGATGGGAAG TTCCGGGCCA GACCGGCCTT CATGGCTTTC
    TCTGCAGGAA AGCGAGTTTG CGCCGGGGAG AGTCTGGCCC GAATGGAGCT CTTCCTCCTG
    TTCTGCTCTC TCCTTCAGAA ATTCACCTTC CGTCGGGCCC CTGGGTCAGA ACCACGGGAC
    TGTACCTACC TGAGGAAGAA CAAGGTCCAG ACCATCATGT CCTCCATAGT CTGCGCTGTT
    CCCCGTTCCA CCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012810134.1
    CDS50..1747
    Translation

    Target ORF information:

    RefSeq Version XM_012954680.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis cytochrome P450, family 2. subfamily c, polypeptide 37 (cyp2c37), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGATTT TTTTATGTTC TCGGTTTAAT GAGTCCCCGG AGCCGGTGCC GCACGTTGTA 
    TCAACAACAC AAATACCATC GGCTCTTTAC TGGGGCAAAG TCCAACCTCA TTCCTATATA
    AACTGGGTAA GAGCAGCAGC GATCTATAGC CTGTCTATTA CCCCCACAGG CAGTGACAGA
    GCCAACGCCA GCCGGGGTAT CAGGACCATG GGGGTTGTTA CCATCTTGTC CCTCGCTCTC
    TCCTTCATCC TCACTCTCCT CTTCCTCGTT TCCTATTGGA GGCAGCAGAA GAAATCTATC
    ACCCTCCCTC CCGGTCCTGC CCCTTTGCCC CTTCTGGGGA ACCTGCGCTA CACCCTACAT
    AAAAGCCACT ACAAATTCTT CCCAGAGCTC AGCAAGCGGT ACGGCCCAGT GTTCACCATC
    TGGCAGATGA CGGATCCGGT GGTGGTTCTG TGCGGGTACG AGATGGTGAG AGACGCCCTG
    ATGAATCACG CAGAGCAGTT TAGCGGTCGC CCCTTTTCCC CGGTGATCGA CCTTTACTCC
    AAGGGATACA GCTTCCCGAG CCTTCAGGGC GAGCGCTGGC GCCAACTCCG ACGCTTCACT
    CTCTCCTCCC TGCGGAACTT TGGGATGGGG AAGAAATCCA TGGAGGAACT GGTACTGGAA
    GAGGCACAAC ATCTCAACGC AGCGGTGTCT GAAACAGGAG GGAAGCCGTT TAACCCGGTT
    CACCTAACGG GCTGCGCCGT AGCCAATATA ACCAGCCGGG CGCTGCTGGG GGAGCAGTTC
    CAGTACCAGG ACCAGAAGTT ACGGGACCTT CTCCTAACAA CACGGAGATT CATCAGCAAC
    ACCCACTCCT TCCTACACCA GCTCAGTAAC ATGTTCCCCG TGCTCCTGTA TGTTCCGGCC
    TTCAGGCAGA AGATTTTCAG AGAAAGTTCC GAGCTGCTCG CATTTGTCAC GGAATATATT
    GAGCAGCACA AACAGACACT GGATCCGAAT TCTCCCAGGG ACTTTATTGA CTACTTCTTA
    TTGAAGATCA GAGAGGAGAA GATGGCGGCA CAAAGCAATT TCTGTGAGAC CAGCCTGCTA
    ATGACCATTA TAGCGCTACT CGCTGCCGGC ACCGAGACCA CCTCATCCAC CCTAGCGTTC
    TGCCTGGCTT TCATATCTAA CTACCCCGAC GCCCAGGCCA AGATACAGAG AGAGATGGAC
    GAGGTTGTGG GCCCCCAGCG CCCCCCAGAG ACGGGGGACC GAGCCCGCCT GCCCTACACC
    AACGCCGTTA TCCACGAGAT GCAGCGGCTG CTCGACTTGG CGCCCATCGC CCATTTTCAC
    GCCGTCACCG AGGACACGGA GTTCCAGGGA TTTACTATCC CCAAGGGAAC CACCGTTATC
    CCCTTCATCT CCTCGGTGCT GTTCGACCCG ACCCAGTGGG AAACCCCTGA GGAGTTCAAC
    CCGGGGCATT TTCTGGATGG TGATGGGAAG TTCCGGGCCA GACCGGCCTT CATGGCTTTC
    TCTGCAGGAA AGCGAGTTTG CGCCGGGGAG AGTCTGGCCC GAATGGAGCT CTTCCTCCTG
    TTCTGCTCTC TCCTTCAGAA ATTCACCTTC CGTCGGGCCC CTGGGTCAGA ACCACGGGAC
    TGTACCTACC TGAGGAAGAA CAAGGTCCAG ACCATCATGT CCTCCATAGT CTGCGCTGTT
    CCCCGTTCCA CCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27341
    Clone ID Related Accession (Same CDS sequence) XM_012954680.1 , XM_031904481.1
    Accession Version XM_031904481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cytochrome P450 2C3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGATTT TTTTATGTTC TCGGTTTAAT GAGTCCCCGG AGCCGGTGCC GCACGTTGTA 
    TCAACAACAC AAATACCATC GGCTCTTTAC TGGGGCAAAG TCCAACCTCA TTCCTATATA
    AACTGGGTAA GAGCAGCAGC GATCTATAGC CTGTCTATTA CCCCCACAGG CAGTGACAGA
    GCCAACGCCA GCCGGGGTAT CAGGACCATG GGGGTTGTTA CCATCTTGTC CCTCGCTCTC
    TCCTTCATCC TCACTCTCCT CTTCCTCGTT TCCTATTGGA GGCAGCAGAA GAAATCTATC
    ACCCTCCCTC CCGGTCCTGC CCCTTTGCCC CTTCTGGGGA ACCTGCGCTA CACCCTACAT
    AAAAGCCACT ACAAATTCTT CCCAGAGCTC AGCAAGCGGT ACGGCCCAGT GTTCACCATC
    TGGCAGATGA CGGATCCGGT GGTGGTTCTG TGCGGGTACG AGATGGTGAG AGACGCCCTG
    ATGAATCACG CAGAGCAGTT TAGCGGTCGC CCCTTTTCCC CGGTGATCGA CCTTTACTCC
    AAGGGATACA GCTTCCCGAG CCTTCAGGGC GAGCGCTGGC GCCAACTCCG ACGCTTCACT
    CTCTCCTCCC TGCGGAACTT TGGGATGGGG AAGAAATCCA TGGAGGAACT GGTACTGGAA
    GAGGCACAAC ATCTCAACGC AGCGGTGTCT GAAACAGGAG GGAAGCCGTT TAACCCGGTT
    CACCTAACGG GCTGCGCCGT AGCCAATATA ACCAGCCGGG CGCTGCTGGG GGAGCAGTTC
    CAGTACCAGG ACCAGAAGTT ACGGGACCTT CTCCTAACAA CACGGAGATT CATCAGCAAC
    ACCCACTCCT TCCTACACCA GCTCAGTAAC ATGTTCCCCG TGCTCCTGTA TGTTCCGGCC
    TTCAGGCAGA AGATTTTCAG AGAAAGTTCC GAGCTGCTCG CATTTGTCAC GGAATATATT
    GAGCAGCACA AACAGACACT GGATCCGAAT TCTCCCAGGG ACTTTATTGA CTACTTCTTA
    TTGAAGATCA GAGAGGAGAA GATGGCGGCA CAAAGCAATT TCTGTGAGAC CAGCCTGCTA
    ATGACCATTA TAGCGCTACT CGCTGCCGGC ACCGAGACCA CCTCATCCAC CCTAGCGTTC
    TGCCTGGCTT TCATATCTAA CTACCCCGAC GCCCAGGCCA AGATACAGAG AGAGATGGAC
    GAGGTTGTGG GCCCCCAGCG CCCCCCAGAG ACGGGGGACC GAGCCCGCCT GCCCTACACC
    AACGCCGTTA TCCACGAGAT GCAGCGGCTG CTCGACTTGG CGCCCATCGC CCATTTTCAC
    GCCGTCACCG AGGACACGGA GTTCCAGGGA TTTACTATCC CCAAGGGAAC CACCGTTATC
    CCCTTCATCT CCTCGGTGCT GTTCGACCCG ACCCAGTGGG AAACCCCTGA GGAGTTCAAC
    CCGGGGCATT TTCTGGATGG TGATGGGAAG TTCCGGGCCA GACCGGCCTT CATGGCTTTC
    TCTGCAGGAA AGCGAGTTTG CGCCGGGGAG AGTCTGGCCC GAATGGAGCT CTTCCTCCTG
    TTCTGCTCTC TCCTTCAGAA ATTCACCTTC CGTCGGGCCC CTGGGTCAGA ACCACGGGAC
    TGTACCTACC TGAGGAAGAA CAAGGTCCAG ACCATCATGT CCTCCATAGT CTGCGCTGTT
    CCCCGTTCCA CCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760341.1
    CDS133..1830
    Translation

    Target ORF information:

    RefSeq Version XM_031904481.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis cytochrome P450, family 2. subfamily c, polypeptide 37 (cyp2c37), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGATTT TTTTATGTTC TCGGTTTAAT GAGTCCCCGG AGCCGGTGCC GCACGTTGTA 
    TCAACAACAC AAATACCATC GGCTCTTTAC TGGGGCAAAG TCCAACCTCA TTCCTATATA
    AACTGGGTAA GAGCAGCAGC GATCTATAGC CTGTCTATTA CCCCCACAGG CAGTGACAGA
    GCCAACGCCA GCCGGGGTAT CAGGACCATG GGGGTTGTTA CCATCTTGTC CCTCGCTCTC
    TCCTTCATCC TCACTCTCCT CTTCCTCGTT TCCTATTGGA GGCAGCAGAA GAAATCTATC
    ACCCTCCCTC CCGGTCCTGC CCCTTTGCCC CTTCTGGGGA ACCTGCGCTA CACCCTACAT
    AAAAGCCACT ACAAATTCTT CCCAGAGCTC AGCAAGCGGT ACGGCCCAGT GTTCACCATC
    TGGCAGATGA CGGATCCGGT GGTGGTTCTG TGCGGGTACG AGATGGTGAG AGACGCCCTG
    ATGAATCACG CAGAGCAGTT TAGCGGTCGC CCCTTTTCCC CGGTGATCGA CCTTTACTCC
    AAGGGATACA GCTTCCCGAG CCTTCAGGGC GAGCGCTGGC GCCAACTCCG ACGCTTCACT
    CTCTCCTCCC TGCGGAACTT TGGGATGGGG AAGAAATCCA TGGAGGAACT GGTACTGGAA
    GAGGCACAAC ATCTCAACGC AGCGGTGTCT GAAACAGGAG GGAAGCCGTT TAACCCGGTT
    CACCTAACGG GCTGCGCCGT AGCCAATATA ACCAGCCGGG CGCTGCTGGG GGAGCAGTTC
    CAGTACCAGG ACCAGAAGTT ACGGGACCTT CTCCTAACAA CACGGAGATT CATCAGCAAC
    ACCCACTCCT TCCTACACCA GCTCAGTAAC ATGTTCCCCG TGCTCCTGTA TGTTCCGGCC
    TTCAGGCAGA AGATTTTCAG AGAAAGTTCC GAGCTGCTCG CATTTGTCAC GGAATATATT
    GAGCAGCACA AACAGACACT GGATCCGAAT TCTCCCAGGG ACTTTATTGA CTACTTCTTA
    TTGAAGATCA GAGAGGAGAA GATGGCGGCA CAAAGCAATT TCTGTGAGAC CAGCCTGCTA
    ATGACCATTA TAGCGCTACT CGCTGCCGGC ACCGAGACCA CCTCATCCAC CCTAGCGTTC
    TGCCTGGCTT TCATATCTAA CTACCCCGAC GCCCAGGCCA AGATACAGAG AGAGATGGAC
    GAGGTTGTGG GCCCCCAGCG CCCCCCAGAG ACGGGGGACC GAGCCCGCCT GCCCTACACC
    AACGCCGTTA TCCACGAGAT GCAGCGGCTG CTCGACTTGG CGCCCATCGC CCATTTTCAC
    GCCGTCACCG AGGACACGGA GTTCCAGGGA TTTACTATCC CCAAGGGAAC CACCGTTATC
    CCCTTCATCT CCTCGGTGCT GTTCGACCCG ACCCAGTGGG AAACCCCTGA GGAGTTCAAC
    CCGGGGCATT TTCTGGATGG TGATGGGAAG TTCCGGGCCA GACCGGCCTT CATGGCTTTC
    TCTGCAGGAA AGCGAGTTTG CGCCGGGGAG AGTCTGGCCC GAATGGAGCT CTTCCTCCTG
    TTCTGCTCTC TCCTTCAGAA ATTCACCTTC CGTCGGGCCC CTGGGTCAGA ACCACGGGAC
    TGTACCTACC TGAGGAAGAA CAAGGTCCAG ACCATCATGT CCTCCATAGT CTGCGCTGTT
    CCCCGTTCCA CCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
    Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,


    Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
    Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
    Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P