ORF » Species Summary » Xenopus tropicalis » kars1 cDNA ORF clone

kars1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01359 NM_001001255.1
Latest version!		 Xenopus tropicalis lysyl-tRNA synthetase 1 (kars1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa26308 XM_012960797.2
Latest version!		 Xenopus tropicalis lysyl-tRNA synthetase (kars), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa55922 XM_012960797.3
Latest version!		 Xenopus tropicalis lysyl-tRNA synthetase 1 (kars1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    kars1 ( NM_001001255.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001001255.1 Xenopus tropicalis lysyl-tRNA synthetase 1 (kars1), mRNA.
    Price & Availability↑
    CloneID OXa01359
    Clone ID Related Accession (Same CDS sequence) NM_001001255.1
    Accession Version NM_001001255.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine--tRNA ligase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067987.1. ##Evidence-Data-START## Transcript exon combination :: BC067987.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGGATG TAGAGGCAGC TGCTCCTGGT AACTCTGAGC CCAAACTGAG CAAGAATGAG 
    CAAAAGAGGC GATTGAAAGC TGAAAAGAAA GCCACTGAAA AGGAAGCCAA GCACAAAGAA 
    CAGAATGAGA AACTGCACAC AGAAGAAGCT GCTATTGATG AGGAAACCTT GGATCCTAAT 
    CAATATTTCA AGATTCGTAC ACAGGCAATT CAGCAGCTGA AAGGCACAAA GGATGACCCC 
    TATCCTCACA AGTACCATGT TGATATGTCC CTCACAGAGT ACATTCAGAA GTACAACAAC 
    CTTCAGACTG GAGACCAGTT ATCAGAAGTT GTCAGTATAG CAGGTCGAAT CCATGCCAAG 
    CGTATTTCTG GAGCTAAACT AATTTTCTAT GATTTGCGAG GTGAAGGAAC AAAGTTGCAA 
    GTGATGGCCA ATTCACGATT TTACAAATCT GAGGAAGAAT TTGCAACAAT TAATAACAAG 
    CTCCGTAGGG GTGATTTGAT TGGAGTTAAA GGAAATCCTG GAAAAACTAA AAAAGGGGAA 
    CTGAGTATCA TACCACATGA AATGATTCTG CTTTCCCCAT GTCTTCATAT GCTTCCACAT 
    CTCCACTTTG GATTGAAAGA CAAGGAAACT AGATATCGAC AGCGATACCT GGATCTTATC 
    TTGAATGACT ATGTGAGACA GAAATTCATT GTTCGTGCCA AGCTTATAAC ATATCTTAGA 
    AAATTCCTTG ATGATTTAGG ATTCCTTGAG ATTGAAACTC CAATGATGAA CGTTATTCCG 
    GGAGGTGCAG TAGCCAAACC GTTCATTACC TATCACAATG AGTTGGACAT GAAGCTATAT 
    ATGAGAATAG CTCCTGAGCT ATACCACAAG ATGCTTGTGG TTGGTGGAAT TGACAGAGTC 
    TATGAAATAG GCAGGCAGTT CCGAAACGAG GGAATTGACC TCACCCACAA TCCAGAGTTC 
    ACAACATGTG AATTTTACAT GGCATATGCA GACTACTATG ATCTCATGGA AATCACAGAA 
    AAATTGCTCT CAGGTATGGT TAAACATATT ACTGGTGGTT ACAAAGTAAT CTACCACCCT 
    GAGGGACCAG AAGGCCCAGA ATATGAGATT GACTTCACTC CACCCTTCAG AAGAATAAGT 
    ATGGTACATG AACTTGAAAA AGCCTTGGGG AAAAAATTGC CTGCAACAGA CCAGTTCGAC 
    ACAGAAGAAA CCCGCAAACT TCTTGATGAC ATTTGTGTGG AAAAAGAGGT TGAGTGCCCA 
    GCACCAAGAA CCACTGCCAG GCTGCTAGAC AAGCTCGTTG GAGATTTCCT GGAGGTTACC 
    TGTATTAATC CAACATATAT TTGCGACCAT CCACAAATTA TGAGCCCTCT TGCAAAATGG 
    CACCGATCTG AAAAGGGACT TACAGAGAGG TTTGAACTTT TTGTAATGAA AAAGGAGATC 
    TGCAACGCCT ATACAGAGTT GAATGATCCA GTCAGGCAAA GGGAGCTGTT TGAAGATCAA 
    GCTAAGGCCA AAGCAGCAGG TGATGATGAG GCAATGTTCA TTGATGAGAC TTTCTGCACT 
    GCACTAGAGT ATGGGCTTCC ACCAACTGCT GGATGGGGAA TGGGCATTGA TCGAGTGGCC 
    ATGTTTCTTA CAGATTCCAA TAACATAAAG GAGGTTTTAC TCTTTCCTGC CATGAAGCCT 
    GAAGATAATA AGAAAGATCC TAATTCAACC CCAAATTCTG ATGGGACATC TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001001255.1
    CDS13..1749
    Translation

    Target ORF information:

    RefSeq Version NM_001001255.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysyl-tRNA synthetase 1 (kars1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001001255.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGGATG TAGAGGCAGC TGCTCCTGGT AACTCTGAGC CCAAACTGAG CAAGAATGAG 
    CAAAAGAGGC GATTGAAAGC TGAAAAGAAA GCCACTGAAA AGGAAGCCAA GCACAAAGAA 
    CAGAATGAGA AACTGCACAC AGAAGAAGCT GCTATTGATG AGGAAACCTT GGATCCTAAT 
    CAATATTTCA AGATTCGTAC ACAGGCAATT CAGCAGCTGA AAGGCACAAA GGATGACCCC 
    TATCCTCACA AGTACCATGT TGATATGTCC CTCACAGAGT ACATTCAGAA GTACAACAAC 
    CTTCAGACTG GAGACCAGTT ATCAGAAGTT GTCAGTATAG CAGGTCGAAT CCATGCCAAG 
    CGTATTTCTG GAGCTAAACT AATTTTCTAT GATTTGCGAG GTGAAGGAAC AAAGTTGCAA 
    GTGATGGCCA ATTCACGATT TTACAAATCT GAGGAAGAAT TTGCAACAAT TAATAACAAG 
    CTCCGTAGGG GTGATTTGAT TGGAGTTAAA GGAAATCCTG GAAAAACTAA AAAAGGGGAA 
    CTGAGTATCA TACCACATGA AATGATTCTG CTTTCCCCAT GTCTTCATAT GCTTCCACAT 
    CTCCACTTTG GATTGAAAGA CAAGGAAACT AGATATCGAC AGCGATACCT GGATCTTATC 
    TTGAATGACT ATGTGAGACA GAAATTCATT GTTCGTGCCA AGCTTATAAC ATATCTTAGA 
    AAATTCCTTG ATGATTTAGG ATTCCTTGAG ATTGAAACTC CAATGATGAA CGTTATTCCG 
    GGAGGTGCAG TAGCCAAACC GTTCATTACC TATCACAATG AGTTGGACAT GAAGCTATAT 
    ATGAGAATAG CTCCTGAGCT ATACCACAAG ATGCTTGTGG TTGGTGGAAT TGACAGAGTC 
    TATGAAATAG GCAGGCAGTT CCGAAACGAG GGAATTGACC TCACCCACAA TCCAGAGTTC 
    ACAACATGTG AATTTTACAT GGCATATGCA GACTACTATG ATCTCATGGA AATCACAGAA 
    AAATTGCTCT CAGGTATGGT TAAACATATT ACTGGTGGTT ACAAAGTAAT CTACCACCCT 
    GAGGGACCAG AAGGCCCAGA ATATGAGATT GACTTCACTC CACCCTTCAG AAGAATAAGT 
    ATGGTACATG AACTTGAAAA AGCCTTGGGG AAAAAATTGC CTGCAACAGA CCAGTTCGAC 
    ACAGAAGAAA CCCGCAAACT TCTTGATGAC ATTTGTGTGG AAAAAGAGGT TGAGTGCCCA 
    GCACCAAGAA CCACTGCCAG GCTGCTAGAC AAGCTCGTTG GAGATTTCCT GGAGGTTACC 
    TGTATTAATC CAACATATAT TTGCGACCAT CCACAAATTA TGAGCCCTCT TGCAAAATGG 
    CACCGATCTG AAAAGGGACT TACAGAGAGG TTTGAACTTT TTGTAATGAA AAAGGAGATC 
    TGCAACGCCT ATACAGAGTT GAATGATCCA GTCAGGCAAA GGGAGCTGTT TGAAGATCAA 
    GCTAAGGCCA AAGCAGCAGG TGATGATGAG GCAATGTTCA TTGATGAGAC TTTCTGCACT 
    GCACTAGAGT ATGGGCTTCC ACCAACTGCT GGATGGGGAA TGGGCATTGA TCGAGTGGCC 
    ATGTTTCTTA CAGATTCCAA TAACATAAAG GAGGTTTTAC TCTTTCCTGC CATGAAGCCT 
    GAAGATAATA AGAAAGATCC TAATTCAACC CCAAATTCTG ATGGGACATC TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    kars1 ( XM_012960797.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816251.1 Xenopus tropicalis lysyl-tRNA synthetase (kars), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa26308
    Clone ID Related Accession (Same CDS sequence) XM_012960797.2
    Accession Version XM_012960797.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine--tRNA ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGGTAC CAGCGCTGCT CAGGCTGGTT AGATACCCAA TCTGGGTACC AAGAGTTCTA 
    GAGGTGGATT TCCATGGAAA GCATCTATCT ACTTTCTTTG CTCTCTACAA GGACCAGGCA 
    TTTCTTGGGA AAAAAAGTGA GCAAAAGAGG CGATTGAAAG CTGAAAAGAA AGCCACTGAA 
    AAGGAAGCCA AGCACAAAGA ACAGAATGAG AAACTGCACA CAGAAGAAGC TGCTATTGAT 
    GAGGAAACCT TGGATCCTAA TCAATATTTC AAGATTCGTA CACAGGCAAT TCAGCAGCTG 
    AAAGGCACAA AGGATGACCC CTATCCTCAC AAGTACCATG TTGATATGTC CCTCACAGAG 
    TACATTCAGA AGTACAACAA CCTTCAGACT GGAGACCAGT TATCAGAAGT TGTCAGTATA 
    GCAGGTCGAA TCCATGCCAA GCGTATTTCT GGAGCTAAAC TAATTTTCTA TGATTTGCGA 
    GGTGAAGGAA CAAAGTTGCA AGTGATGGCC AATTCACGAT TTTACAAATC TGAGGAAGAA 
    TTTGCAACAA TTAATAACAA GCTCCGTAGG GGTGATTTGA TTGGAGTTAA AGGAAATCCT 
    GGAAAAACTA AAAAAGGGGA ACTGAGTATC ATACCACATG AAATGATTCT GCTTTCCCCA 
    TGTCTTCATA TGCTTCCACA TCTCCACTTT GGATTGAAAG ACAAGGAAAC TAGATATCGA 
    CAGCGATACC TGGATCTTAT CTTGAATGAC TATGTGAGAC AGAAATTCAT TGTTCGTGCC 
    AAGCTTATAA CATATCTTAG AAAATTCCTT GATGATTTAG GATTCCTTGA GATTGAAACT 
    CCAATGATGA ACGTTATTCC GGGAGGTGCA GTAGCCAAAC CGTTCATTAC CTATCACAAT 
    GAGTTGGACA TGAAGCTATA TATGAGAATA GCTCCTGAGC TATACCACAA GATGCTTGTG 
    GTTGGTGGAA TTGACAGAGT CTATGAAATA GGCAGGCAGT TCCGAAACGA GGGAATTGAC 
    CTCACCCACA ATCCAGAGTT CACAACATGT GAATTTTACA TGGCATATGC AGACTACAAT 
    GATCTCATGG AAATCACAGA AAAATTGCTC TCAGGTATGG TTAAACATAT TACTGGTGGT 
    TACAAAGTAA TCTACCACCC TGAGGGACCA GAAGGCCCAG AATATGAGAT TGACTTCACT 
    CCACCCTTCA GAAGAATAAG TATGGTACAT GAACTTGAAA AAGCCTTGGG GAAAAAATTG 
    CCTGCAACAG ACCAGTTCGA CACAGAAGAA ACCCGCAAAC TTCTTGATGA CATTTGTGTG 
    GAAAAAGAGG TTGAGTGCCC AGCACCAAGA ACCACTGCCA GGCTGCTAGA CAAGCTCGTT 
    GGAGATTTCC TGGAGGTTAC CTGTATTAAT CCAACATATA TTTGCGACCA TCCACAAATT 
    ATGAGCCCTC TTGCAAAATG GCACCGATCT GAAAAGGGAC TTACAGAGAG GTTTGAACTT 
    TTTGTAATGA AAAAGGAGAT CTGCAACGCC TATACAGAGT TGAATGATCC AGTCAGGCAA 
    AGGGAGCTGT TTGAAGATCA AGCTAAGGCC AAAGCAGCAG GTGATGATGA GGCAATGTTC 
    ATTGATGAGA CTTTCTGCAC TGCACTAGAG TATGGGCTTC CACCAACTGC TGGATGGGGA 
    ATGGGCATTG ATCGAGTGGC CATGTTTCTT ACAGATTCCA ATAACATAAA GGAGGTTTTA 
    CTCTTTCCTG CCATGAAGCC TGAAGATAAT AAGAAAGATC CTAATTCAAC CCCAAATTCT 
    GATGGGACAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816251.1
    CDS178..1995
    Translation

    Target ORF information:

    RefSeq Version XM_012960797.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysyl-tRNA synthetase (kars), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012960797.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGGTAC CAGCGCTGCT CAGGCTGGTT AGATACCCAA TCTGGGTACC AAGAGTTCTA 
    GAGGTGGATT TCCATGGAAA GCATCTATCT ACTTTCTTTG CTCTCTACAA GGACCAGGCA 
    TTTCTTGGGA AAAAAAGTGA GCAAAAGAGG CGATTGAAAG CTGAAAAGAA AGCCACTGAA 
    AAGGAAGCCA AGCACAAAGA ACAGAATGAG AAACTGCACA CAGAAGAAGC TGCTATTGAT 
    GAGGAAACCT TGGATCCTAA TCAATATTTC AAGATTCGTA CACAGGCAAT TCAGCAGCTG 
    AAAGGCACAA AGGATGACCC CTATCCTCAC AAGTACCATG TTGATATGTC CCTCACAGAG 
    TACATTCAGA AGTACAACAA CCTTCAGACT GGAGACCAGT TATCAGAAGT TGTCAGTATA 
    GCAGGTCGAA TCCATGCCAA GCGTATTTCT GGAGCTAAAC TAATTTTCTA TGATTTGCGA 
    GGTGAAGGAA CAAAGTTGCA AGTGATGGCC AATTCACGAT TTTACAAATC TGAGGAAGAA 
    TTTGCAACAA TTAATAACAA GCTCCGTAGG GGTGATTTGA TTGGAGTTAA AGGAAATCCT 
    GGAAAAACTA AAAAAGGGGA ACTGAGTATC ATACCACATG AAATGATTCT GCTTTCCCCA 
    TGTCTTCATA TGCTTCCACA TCTCCACTTT GGATTGAAAG ACAAGGAAAC TAGATATCGA 
    CAGCGATACC TGGATCTTAT CTTGAATGAC TATGTGAGAC AGAAATTCAT TGTTCGTGCC 
    AAGCTTATAA CATATCTTAG AAAATTCCTT GATGATTTAG GATTCCTTGA GATTGAAACT 
    CCAATGATGA ACGTTATTCC GGGAGGTGCA GTAGCCAAAC CGTTCATTAC CTATCACAAT 
    GAGTTGGACA TGAAGCTATA TATGAGAATA GCTCCTGAGC TATACCACAA GATGCTTGTG 
    GTTGGTGGAA TTGACAGAGT CTATGAAATA GGCAGGCAGT TCCGAAACGA GGGAATTGAC 
    CTCACCCACA ATCCAGAGTT CACAACATGT GAATTTTACA TGGCATATGC AGACTACAAT 
    GATCTCATGG AAATCACAGA AAAATTGCTC TCAGGTATGG TTAAACATAT TACTGGTGGT 
    TACAAAGTAA TCTACCACCC TGAGGGACCA GAAGGCCCAG AATATGAGAT TGACTTCACT 
    CCACCCTTCA GAAGAATAAG TATGGTACAT GAACTTGAAA AAGCCTTGGG GAAAAAATTG 
    CCTGCAACAG ACCAGTTCGA CACAGAAGAA ACCCGCAAAC TTCTTGATGA CATTTGTGTG 
    GAAAAAGAGG TTGAGTGCCC AGCACCAAGA ACCACTGCCA GGCTGCTAGA CAAGCTCGTT 
    GGAGATTTCC TGGAGGTTAC CTGTATTAAT CCAACATATA TTTGCGACCA TCCACAAATT 
    ATGAGCCCTC TTGCAAAATG GCACCGATCT GAAAAGGGAC TTACAGAGAG GTTTGAACTT 
    TTTGTAATGA AAAAGGAGAT CTGCAACGCC TATACAGAGT TGAATGATCC AGTCAGGCAA 
    AGGGAGCTGT TTGAAGATCA AGCTAAGGCC AAAGCAGCAG GTGATGATGA GGCAATGTTC 
    ATTGATGAGA CTTTCTGCAC TGCACTAGAG TATGGGCTTC CACCAACTGC TGGATGGGGA 
    ATGGGCATTG ATCGAGTGGC CATGTTTCTT ACAGATTCCA ATAACATAAA GGAGGTTTTA 
    CTCTTTCCTG CCATGAAGCC TGAAGATAAT AAGAAAGATC CTAATTCAAC CCCAAATTCT 
    GATGGGACAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    kars1 ( XM_012960797.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816251.2 Xenopus tropicalis lysyl-tRNA synthetase 1 (kars1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa55922
    Clone ID Related Accession (Same CDS sequence) XM_012960797.3
    Accession Version XM_012960797.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine--tRNA ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGGTAC CAGCGCTGCT CAGGCTGGTT AGATACCCAA TCTGGGTACC AAGAGTTCTA 
    GAGGTGGATT TCCATGGAAA GCATCTATCT ACTTTCTTTG CTCTCTACAA GGACCAGGCA 
    TTTCTTGGGA AAAAAAGTGA GCAAAAGAGG CGATTGAAAG CTGAAAAGAA AGCCACTGAA 
    AAGGAAGCCA AGCACAAAGA ACAGAATGAG AAACTGCACA CAGAAGAAGC TGCTATTGAT 
    GAGGAAACCT TGGATCCTAA TCAATATTTC AAGATTCGTA CACAGGCAAT TCAGCAGCTG 
    AAAGGCACAA AGGATGACCC CTATCCTCAC AAGTACCATG TTGATATGTC CCTCACAGAG 
    TACATTCAGA AGTACAACAA CCTTCAGACT GGAGACCAGT TATCAGAAGT TGTCAGTATA 
    GCAGGTCGAA TCCATGCCAA GCGTATTTCT GGAGCTAAAC TAATTTTCTA TGATTTGCGA 
    GGTGAAGGAA CAAAGTTGCA AGTGATGGCC AATTCACGAT TTTACAAATC TGAGGAAGAA 
    TTTGCAACAA TTAATAACAA GCTCCGTAGG GGTGATTTGA TTGGAGTTAA AGGAAATCCT 
    GGAAAAACTA AAAAAGGGGA ACTGAGTATC ATACCACATG AAATGATTCT GCTTTCCCCA 
    TGTCTTCATA TGCTTCCACA TCTCCACTTT GGATTGAAAG ACAAGGAAAC TAGATATCGA 
    CAGCGATACC TGGATCTTAT CTTGAATGAC TATGTGAGAC AGAAATTCAT TGTTCGTGCC 
    AAGCTTATAA CATATCTTAG AAAATTCCTT GATGATTTAG GATTCCTTGA GATTGAAACT 
    CCAATGATGA ACGTTATTCC GGGAGGTGCA GTAGCCAAAC CGTTCATTAC CTATCACAAT 
    GAGTTGGACA TGAAGCTATA TATGAGAATA GCTCCTGAGC TATACCACAA GATGCTTGTG 
    GTTGGTGGAA TTGACAGAGT CTATGAAATA GGCAGGCAGT TCCGAAACGA GGGAATTGAC 
    CTCACCCACA ATCCAGAGTT CACAACATGT GAATTTTACA TGGCATATGC AGACTACTAT 
    GATCTCATGG AAATCACAGA AAAATTGCTC TCAGGTATGG TTAAACATAT TACTGGTGGT 
    TACAAAGTAA TCTACCACCC TGAGGGACCA GAAGGCCCAG AATATGAGAT TGACTTCACT 
    CCACCCTTCA GAAGAATAAG TATGGTACAT GAACTTGAAA AAGCCTTGGG GAAAAAATTG 
    CCTGCAACAG ACCAGTTCGA CACAGAAGAA ACCCGCAAAC TTCTTGATGA CATTTGTGTG 
    GAAAAAGAGG TTGAGTGCCC AGCACCAAGA ACCACTGCCA GGCTGCTAGA CAAGCTCGTT 
    GGAGATTTCC TGGAGGTTAC CTGTATTAAT CCAACATATA TTTGCGACCA TCCACAAATT 
    ATGAGCCCTC TTGCAAAATG GCACCGATCT GAAAAGGGAC TTACAGAGAG GTTTGAACTT 
    TTTGTAATGA AAAAGGAGAT CTGCAACGCC TATACAGAGT TGAATGATCC AGTCAGGCAA 
    AGGGAGCTGT TTGAAGATCA AGCTAAGGCC AAAGCAGCAG GTGATGATGA GGCAATGTTC 
    ATTGATGAGA CTTTCTGCAC TGCACTAGAG TATGGGCTTC CACCAACTGC TGGATGGGGA 
    ATGGGCATTG ATCGAGTGGC CATGTTTCTT ACAGATTCCA ATAACATAAA GGAGGTTTTA 
    CTCTTTCCTG CCATGAAGCC TGAAGATAAT AAGAAAGATC CTAATTCAAC CCCAAATTCT 
    GATGGGACAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012816251.2
    CDS176..1993
    Translation

    Target ORF information:

    RefSeq Version XM_012960797.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysyl-tRNA synthetase 1 (kars1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012960797.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTGGTAC CAGCGCTGCT CAGGCTGGTT AGATACCCAA TCTGGGTACC AAGAGTTCTA 
    GAGGTGGATT TCCATGGAAA GCATCTATCT ACTTTCTTTG CTCTCTACAA GGACCAGGCA 
    TTTCTTGGGA AAAAAAGTGA GCAAAAGAGG CGATTGAAAG CTGAAAAGAA AGCCACTGAA 
    AAGGAAGCCA AGCACAAAGA ACAGAATGAG AAACTGCACA CAGAAGAAGC TGCTATTGAT 
    GAGGAAACCT TGGATCCTAA TCAATATTTC AAGATTCGTA CACAGGCAAT TCAGCAGCTG 
    AAAGGCACAA AGGATGACCC CTATCCTCAC AAGTACCATG TTGATATGTC CCTCACAGAG 
    TACATTCAGA AGTACAACAA CCTTCAGACT GGAGACCAGT TATCAGAAGT TGTCAGTATA 
    GCAGGTCGAA TCCATGCCAA GCGTATTTCT GGAGCTAAAC TAATTTTCTA TGATTTGCGA 
    GGTGAAGGAA CAAAGTTGCA AGTGATGGCC AATTCACGAT TTTACAAATC TGAGGAAGAA 
    TTTGCAACAA TTAATAACAA GCTCCGTAGG GGTGATTTGA TTGGAGTTAA AGGAAATCCT 
    GGAAAAACTA AAAAAGGGGA ACTGAGTATC ATACCACATG AAATGATTCT GCTTTCCCCA 
    TGTCTTCATA TGCTTCCACA TCTCCACTTT GGATTGAAAG ACAAGGAAAC TAGATATCGA 
    CAGCGATACC TGGATCTTAT CTTGAATGAC TATGTGAGAC AGAAATTCAT TGTTCGTGCC 
    AAGCTTATAA CATATCTTAG AAAATTCCTT GATGATTTAG GATTCCTTGA GATTGAAACT 
    CCAATGATGA ACGTTATTCC GGGAGGTGCA GTAGCCAAAC CGTTCATTAC CTATCACAAT 
    GAGTTGGACA TGAAGCTATA TATGAGAATA GCTCCTGAGC TATACCACAA GATGCTTGTG 
    GTTGGTGGAA TTGACAGAGT CTATGAAATA GGCAGGCAGT TCCGAAACGA GGGAATTGAC 
    CTCACCCACA ATCCAGAGTT CACAACATGT GAATTTTACA TGGCATATGC AGACTACTAT 
    GATCTCATGG AAATCACAGA AAAATTGCTC TCAGGTATGG TTAAACATAT TACTGGTGGT 
    TACAAAGTAA TCTACCACCC TGAGGGACCA GAAGGCCCAG AATATGAGAT TGACTTCACT 
    CCACCCTTCA GAAGAATAAG TATGGTACAT GAACTTGAAA AAGCCTTGGG GAAAAAATTG 
    CCTGCAACAG ACCAGTTCGA CACAGAAGAA ACCCGCAAAC TTCTTGATGA CATTTGTGTG 
    GAAAAAGAGG TTGAGTGCCC AGCACCAAGA ACCACTGCCA GGCTGCTAGA CAAGCTCGTT 
    GGAGATTTCC TGGAGGTTAC CTGTATTAAT CCAACATATA TTTGCGACCA TCCACAAATT 
    ATGAGCCCTC TTGCAAAATG GCACCGATCT GAAAAGGGAC TTACAGAGAG GTTTGAACTT 
    TTTGTAATGA AAAAGGAGAT CTGCAACGCC TATACAGAGT TGAATGATCC AGTCAGGCAA 
    AGGGAGCTGT TTGAAGATCA AGCTAAGGCC AAAGCAGCAG GTGATGATGA GGCAATGTTC 
    ATTGATGAGA CTTTCTGCAC TGCACTAGAG TATGGGCTTC CACCAACTGC TGGATGGGGA 
    ATGGGCATTG ATCGAGTGGC CATGTTTCTT ACAGATTCCA ATAACATAAA GGAGGTTTTA 
    CTCTTTCCTG CCATGAAGCC TGAAGATAAT AAGAAAGATC CTAATTCAAC CCCAAATTCT 
    GATGGGACAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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