kars1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kars1
Entrez Gene ID	280647
Full Name	lysyl-tRNA synthetase
Synonyms	cb530,wu:fa16h02,zgc:92483
General protein information	Preferred Names lysyl-tRNA synthetase Names lysine--tRNA ligase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328506.1	NP_001315435.1	lysine--tRNA ligase isoform 2
NM_001002386.2	NP_001002386.1	lysine--tRNA ligase isoform 1

Pathways from BioSystems

Homologies

	KARS	XP_001104312.2
Schizosaccharomyces pombe (fission yeast)	krs1	NP_595892.1
Neurospora crassa	NCU04020	XP_957635.1
Canis lupus familiaris (dog)	KARS	XP_861533.1
Saccharomyces cerevisiae (baker's yeast)	KRS1	NP_010322.1
Oryza sativa (rice)	Os03g0586800	NP_001050572.1
Xenopus tropicalis (tropical clawed frog)	kars	NP_001001255.1
Homo sapiens (human)	KARS	NP_001123561.1
Mus musculus (house mouse)	Kars	NP_001124340.1
Danio rerio (zebrafish)	kars	NP_001002386.1
Caenorhabditis elegans (roundworm)	kars-1	NP_495453.1
Bos taurus (cattle)	KARS	NP_001029528.1
Rattus norvegicus (Norway rat)	Kars	NP_001006968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007858	XP_317634.3
Kluyveromyces lactis	KLLA0F18392g	XP_455904.1
Eremothecium gossypii	AGOS_AGR037C	NP_986702.1
Arabidopsis thaliana (thale cress)	ATKRS-1	NP_187777.1
Pan troglodytes (chimpanzee)	KARS	XP_003315273.1
Gallus gallus (chicken)	KARS	NP_001025754.1
Drosophila melanogaster (fruit fly)	Aats-lys	NP_727353.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000325	XP_310792.5
Magnaporthe oryzae (rice blast fungus)	MGG_04966	XP_003712462.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases.
Waldron AL, Cahan SH, Franklyn CS, Ebert AM
PloS one12(9)e0185317(2017)

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Smith LC, Clark JC, Bisesi JH Jr, Ferguson PL, Sabo-Attwood T
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following kars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00587	NM_001002386.2 Latest version!	Danio rerio lysyl-tRNA synthetase 1 (kars1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa13630	NM_001328506.1 Latest version!	Danio rerio lysyl-tRNA synthetase 1 (kars1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kars1 ( NM_001002386.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002386.1 Danio rerio lysyl-tRNA synthetase 1 (kars1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa00587
Clone ID Related Accession (Same CDS sequence)	NM_001002386.2
Accession Version	NM_001002386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	lysine--tRNA ligase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01060768.1, BC076028.1 and CU651676.5. On Jun 16, 2016 this sequence version replaced NM_001002386.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01013717.1, BC076028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTGACTC TGGTCAGAAT ATGCCAACTG CAAGCGCTCA GGCCGCGGAC GCTTGCTCTT 
CCCGGCTCAC AAAACCTGCC TTTGCTTATT CAAGGAATAC GCTGGAAAAG TGACAAAAGT 
GAGCTGAAAA GGCGCATGAA AGCTGAGAAG AAAGCAGCTG AGAAGGAAGC CAAGGTCAAA 
GAGCAGCAGG AACAGAAGGA GACCAATGAC AAGCCACAGC AGAACGCATA CGGGGCAGAT 
GAGGAAACAC TGGACCCCAA TCAATACTTC AAGATACGTT CACAAGCCAT TCAAGCCTTG 
AAGGGAACAG CCGAAGATCC TTACCCTCAC AAATTTCACG TGGACTTGTC TCTTACTGAG 
TTCATTGAGC GCTATAACCA CCTTCAGCCT GGAGACCACT TGACAGATGT TGTCCTCAAT 
TTATCAGGTC GTGTTCATGC TAAGAGGGCT TCAGGCGCCA AGCTGCTGTT CTATGATCTG 
CGAGGAGAAG GAGTCAAGCT GCAGGTCATG GCCAACTCCA GAAACTACAC ATCAGAAGAA 
GCGTTTGTGC ACATCAACAA CAAACTGAGG CGTGGAGACA TCATCGGGGT CCGGGGGAAC 
CCTGGCAAGA CAAAGAAGGG CGAGTTGAGC ATCATCCCAG TGGAGATGAC CCTGCTGTCT 
CCATGTCTTC ATATGCTTCC ACATCTTCAC TTTGGCCTGA AAGACAAGGA AACCAGGTTC 
AGACAGCGAT ATCTGGACCT GATTCTTAAT GATTTTGTGA GACAGAAGTT TGTTACTCGT 
TCCAAGATTA TCACGTATCT CCGCAGCTTT CTTGACCAGC TTGGATTTCT GGAGATTGAA 
ACCCCAATGA TGAACCTGAT CCCTGGTGGC GCAGTAGCAA AGCCATTTAT CACCTATCAC 
AATGATCTGA ACATGAACCT GTACATGAGA ATTGCTCCTG AGCTTTACCA TAAGATGTTG 
GTAGTTGGAG GAATCGACCG CGTGTACGAG ATTGGCCGTC AGTTCCGTAA TGAAGGCATC 
GATCTCACTC ACAACCCAGA GTTCACCACC TGTGAGTTCT ACATGGCCTA TGCCGACTAT 
CACGACCTGA TGGAAATCAC AGAGAAGCTT CTCTCAGGAA TGGTGAAGCA CATTACCGGA 
GCTTATAAAG TGACGTATCA TCCTGATGGT CCAGAGGGAC AGGCCTACGA AATAGACTTC 
ACCCCTCCCT TCAGGCGAAT CAGCATGACC CAAGAGCTGG AGAAGGAGTT GGGTGTGAAG 
TTTCCACCTC CTGACACCTA TGACAGTGAC GAAATGCGCA AGTTCTTAGA CGACCTGTGT 
GTTCAGAAAG AAGTTGAATG TCCTCCTCCC AGGACAACTG CTCGACTTCT TGATAAGTTG 
GTGGGAGACT TCCTGGAGGT GAAGTGCATC AACCCAACAT ACATCTGCGA TCATCCACAG 
ATAATGAGTC CTCTTGCTAA ATGGCACAGG TCACAGAAAG GTCTAACTGA ACGTTTTGAA 
CTCTTTGTGA TGAAGAAGGA GATCTGCAAT GCCTACACAG AGTTGAACGA TCCCATCAGA 
CAGCGAGAAC TGTTTGAGCA GCAGGCTAAG GCTAAGGCAG AAGGTGACGA CGAGGCCATG 
TTCATTGATG AGACCTTCTG CACCGCTTTA GAGTACGGTC TTCCCCCCAC AGCAGGATGG 
GGAATGGGCA TCGACCGCCT CACCATGTTC CTCACAGACT CCAACAACAT CAAGGAGGTG 
CTGCTTTTCC CTGCCATGAA GCCAGAAGAC AATAAACCAG CTGCACCTGC AGAGGGCACA 
TCAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002386.1
CDS	175..1983
Translation	MLTLVRICQLQALRPRTLALPGSQNLPLLIQGIRWKSDKSELKRRMKAEKKAAEKEAKVKEQQEQKETNDKPQQNAYGADEETLDPNQYFKIRSQAIQALKGTAEDPYPHKFHVDLSLTEFIERYNHLQPGDHLTDVVLNLSGRVHAKRASGAKLLFYDLRGEGVKLQVMANSRNYTSEEAFVHINNKLRRGDIIGVRGNPGKTKKGELSIIPVEMTLLSPCLHMLPHLHFGLKDKETRFRQRYLDLILNDFVRQKFVTRSKIITYLRSFLDQLGFLEIETPMMNLIPGGAVAKPFITYHNDLNMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKLLSGMVKHITGAYKVTYHPDGPEGQAYEIDFTPPFRRISMTQELEKELGVKFPPPDTYDSDEMRKFLDDLCVQKEVECPPPRTTARLLDKLVGDFLEVKCINPTYICDHPQIMSPLAKWHRSQKGLTERFELFVMKKEICNAYTELNDPIRQRELFEQQAKAKAEGDDEAMFIDETFCTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDNKPAAPAEGTSV

Target ORF information:

RefSeq Version	NM_001002386.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysyl-tRNA synthetase 1 (kars1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002386.2

ORF Insert Sequence:

ATGTTGACTC TGGTCAGAAT ATGCCAACTG CAAGCGCTCA GGCCGCGGAC GCTTGCTCTT 
CCCGGCTCAC AAAACCTGCC TTTGCTTATT CAAGGAATAC GCTGGAAAAG TGACAAAAGT 
GAGCTGAAAA GGCGCATGAA AGCTGAGAAG AAAGCAGCTG AGAAGGAAGC CAAGGTCAAA 
GAGCAGCAGG AACAGAAGGA GACCAATGAC AAGCCACAGC AGAACGCATA CGGGGCAGAT 
GAGGAAACAC TGGACCCCAA TCAATACTTC AAGATACGTT CACAAGCCAT TCAAGCCTTG 
AAGGGAACAG CCGAAGATCC TTACCCTCAC AAATTTCACG TGGACTTGTC TCTTACTGAG 
TTCATTGAGC GCTATAACCA CCTTCAGCCT GGAGACCACT TGACAGATGT TGTCCTCAAT 
TTATCAGGTC GTGTTCATGC TAAGAGGGCT TCAGGCGCCA AGCTGCTGTT CTATGATCTG 
CGAGGAGAAG GAGTCAAGCT GCAGGTCATG GCCAACTCCA GAAACTACAC ATCAGAAGAA 
GCGTTTGTGC ACATCAACAA CAAACTGAGG CGTGGAGACA TCATCGGGGT CCGGGGGAAC 
CCTGGCAAGA CAAAGAAGGG CGAGTTGAGC ATCATCCCAG TGGAGATGAC CCTGCTGTCT 
CCATGTCTTC ATATGCTTCC ACATCTTCAC TTTGGCCTGA AAGACAAGGA AACCAGGTTC 
AGACAGCGAT ATCTGGACCT GATTCTTAAT GATTTTGTGA GACAGAAGTT TGTTACTCGT 
TCCAAGATTA TCACGTATCT CCGCAGCTTT CTTGACCAGC TTGGATTTCT GGAGATTGAA 
ACCCCAATGA TGAACCTGAT CCCTGGTGGC GCAGTAGCAA AGCCATTTAT CACCTATCAC 
AATGATCTGA ACATGAACCT GTACATGAGA ATTGCTCCTG AGCTTTACCA TAAGATGTTG 
GTAGTTGGAG GAATCGACCG CGTGTACGAG ATTGGCCGTC AGTTCCGTAA TGAAGGCATC 
GATCTCACTC ACAACCCAGA GTTCACCACC TGTGAGTTCT ACATGGCCTA TGCCGACTAT 
CACGACCTGA TGGAAATCAC AGAGAAGCTT CTCTCAGGAA TGGTGAAGCA CATTACCGGA 
GCTTATAAAG TGACGTATCA TCCTGATGGT CCAGAGGGAC AGGCCTACGA AATAGACTTC 
ACCCCTCCCT TCAGGCGAAT CAGCATGACC CAAGAGCTGG AGAAGGAGTT GGGTGTGAAG 
TTTCCACCTC CTGACACCTA TGACAGTGAC GAAATGCGCA AGTTCTTAGA CGACCTGTGT 
GTTCAGAAAG AAGTTGAATG TCCTCCTCCC AGGACAACTG CTCGACTTCT TGATAAGTTG 
GTGGGAGACT TCCTGGAGGT GAAGTGCATC AACCCAACAT ACATCTGCGA TCATCCACAG 
ATAATGAGTC CTCTTGCTAA ATGGCACAGG TCACAGAAAG GTCTAACTGA ACGTTTTGAA 
CTCTTTGTGA TGAAGAAGGA GATCTGCAAT GCCTACACAG AGTTGAACGA TCCCATCAGA 
CAGCGAGAAC TGTTTGAGCA GCAGGCTAAG GCTAAGGCAG AAGGTGACGA CGAGGCCATG 
TTCATTGATG AGACCTTCTG CACCGCTTTA GAGTACGGTC TTCCCCCCAC AGCAGGATGG 
GGAATGGGCA TCGACCGCCT CACCATGTTC CTCACAGACT CCAACAACAT CAAGGAGGTG 
CTGCTTTTCC CTGCCATGAA GCCAGAAGAC AATAAACCAG CTGCACCTGC AGAGGGCACA 
TCAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kars1 ( NM_001328506.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315435.1 Danio rerio lysyl-tRNA synthetase 1 (kars1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa13630
Clone ID Related Accession (Same CDS sequence)	NM_001328506.1
Accession Version	NM_001328506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Danio rerio(zebrafish)
Product	lysine--tRNA ligase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01060768.1, BC076028.1 and CU651676.5. On Jun 16, 2016 this sequence version replaced XM_005166665.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01000709.1, AY648786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACG GAGCTGTGGT GGATGGAGAC AAACTCAGCA AAAATGAGCT GAAAAGGCGC 
ATGAAAGCTG AGAAGAAAGC AGCTGAGAAG GAAGCCAAGG TCAAAGAGCA GCAGGAACAG 
AAGGAGACCA ATGACAAGCC ACAGCAGAAC GCATACGGGG CAGATGAGGA AACACTGGAC 
CCCAATCAAT ACTTCAAGAT ACGTTCACAA GCCATTCAAG CCTTGAAGGG AACAGCCGAA 
GATCCTTACC CTCACAAATT TCACGTGGAC TTGTCTCTTA CTGAGTTCAT TGAGCGCTAT 
AACCACCTTC AGCCTGGAGA CCACTTGACA GATGTTGTCC TCAATTTATC AGGTCGTGTT 
CATGCTAAGA GGGCTTCAGG CGCCAAGCTG CTGTTCTATG ATCTGCGAGG AGAAGGAGTC 
AAGCTGCAGG TCATGGCCAA CTCCAGAAAC TACACATCAG AAGAAGCGTT TGTGCACATC 
AACAACAAAC TGAGGCGTGG AGACATCATC GGGGTCCGGG GGAACCCTGG CAAGACAAAG 
AAGGGCGAGT TGAGCATCAT CCCAGTGGAG ATGACCCTGC TGTCTCCATG TCTTCATATG 
CTTCCACATC TTCACTTTGG CCTGAAAGAC AAGGAAACCA GGTTCAGACA GCGATATCTG 
GACCTGATTC TTAATGATTT TGTGAGACAG AAGTTTGTTA CTCGTTCCAA GATTATCACG 
TATCTCCGCA GCTTTCTTGA CCAGCTTGGA TTTCTGGAGA TTGAAACCCC AATGATGAAC 
CTGATCCCTG GTGGCGCAGT AGCAAAGCCA TTTATCACCT ATCACAATGA TCTGAACATG 
AACCTGTACA TGAGAATTGC TCCTGAGCTT TACCATAAGA TGTTGGTAGT TGGAGGAATC 
GACCGCGTGT ACGAGATTGG CCGTCAGTTC CGTAATGAAG GCATCGATCT CACTCACAAC 
CCAGAGTTCA CCACCTGTGA GTTCTACATG GCCTATGCCG ACTATCACGA CCTGATGGAA 
ATCACAGAGA AGCTTCTCTC AGGAATGGTG AAGCACATTA CCGGAGCTTA TAAAGTGACG 
TATCATCCTG ATGGTCCAGA GGGACAGGCC TACGAAATAG ACTTCACCCC TCCCTTCAGG 
CGAATCAGCA TGACCCAAGA GCTGGAGAAG GAGTTGGGTG TGAAGTTTCC ACCTCCTGAC 
ACCTATGACA GTGACGAAAT GCGCAAGTTC TTAGACGACC TGTGTGTTCA GAAAGAAGTT 
GAATGTCCTC CTCCCAGGAC AACTGCTCGA CTTCTTGATA AGTTGGTGGG AGACTTCCTG 
GAGGTGAAGT GCATCAACCC AACATACATC TGCGATCATC CACAGATAAT GAGTCCTCTT 
GCTAAATGGC ACAGGTCACA GAAAGGTCTA ACTGAACGTT TTGAACTCTT TGTGATGAAG 
AAGGAGATCT GCAATGCCTA CACAGAGTTG AACGATCCCA TCAGACAGCG AGAACTGTTT 
GAGCAGCAGG CTAAGGCTAA GGCAGAAGGT GACGACGAGG CCATGTTCAT TGATGAGACC 
TTCTGCACCG CTTTAGAGTA CGGTCTTCCC CCCACAGCAG GATGGGGAAT GGGCATCGAC 
CGCCTCACCA TGTTCCTCAC AGACTCCAAC AACATCAAGG AGGTGCTGCT TTTCCCTGCC 
ATGAAGCCAG AAGACAATAA ACCAGCTGCA CCTGCAGAGG GCACATCAGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315435.1
CDS	119..1852
Translation	MADGAVVDGDKLSKNELKRRMKAEKKAAEKEAKVKEQQEQKETNDKPQQNAYGADEETLDPNQYFKIRSQAIQALKGTAEDPYPHKFHVDLSLTEFIERYNHLQPGDHLTDVVLNLSGRVHAKRASGAKLLFYDLRGEGVKLQVMANSRNYTSEEAFVHINNKLRRGDIIGVRGNPGKTKKGELSIIPVEMTLLSPCLHMLPHLHFGLKDKETRFRQRYLDLILNDFVRQKFVTRSKIITYLRSFLDQLGFLEIETPMMNLIPGGAVAKPFITYHNDLNMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKLLSGMVKHITGAYKVTYHPDGPEGQAYEIDFTPPFRRISMTQELEKELGVKFPPPDTYDSDEMRKFLDDLCVQKEVECPPPRTTARLLDKLVGDFLEVKCINPTYICDHPQIMSPLAKWHRSQKGLTERFELFVMKKEICNAYTELNDPIRQRELFEQQAKAKAEGDDEAMFIDETFCTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDNKPAAPAEGTSV

Target ORF information:

RefSeq Version	NM_001328506.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysyl-tRNA synthetase 1 (kars1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328506.1

ORF Insert Sequence:

ATGGCAGACG GAGCTGTGGT GGATGGAGAC AAACTCAGCA AAAATGAGCT GAAAAGGCGC 
ATGAAAGCTG AGAAGAAAGC AGCTGAGAAG GAAGCCAAGG TCAAAGAGCA GCAGGAACAG 
AAGGAGACCA ATGACAAGCC ACAGCAGAAC GCATACGGGG CAGATGAGGA AACACTGGAC 
CCCAATCAAT ACTTCAAGAT ACGTTCACAA GCCATTCAAG CCTTGAAGGG AACAGCCGAA 
GATCCTTACC CTCACAAATT TCACGTGGAC TTGTCTCTTA CTGAGTTCAT TGAGCGCTAT 
AACCACCTTC AGCCTGGAGA CCACTTGACA GATGTTGTCC TCAATTTATC AGGTCGTGTT 
CATGCTAAGA GGGCTTCAGG CGCCAAGCTG CTGTTCTATG ATCTGCGAGG AGAAGGAGTC 
AAGCTGCAGG TCATGGCCAA CTCCAGAAAC TACACATCAG AAGAAGCGTT TGTGCACATC 
AACAACAAAC TGAGGCGTGG AGACATCATC GGGGTCCGGG GGAACCCTGG CAAGACAAAG 
AAGGGCGAGT TGAGCATCAT CCCAGTGGAG ATGACCCTGC TGTCTCCATG TCTTCATATG 
CTTCCACATC TTCACTTTGG CCTGAAAGAC AAGGAAACCA GGTTCAGACA GCGATATCTG 
GACCTGATTC TTAATGATTT TGTGAGACAG AAGTTTGTTA CTCGTTCCAA GATTATCACG 
TATCTCCGCA GCTTTCTTGA CCAGCTTGGA TTTCTGGAGA TTGAAACCCC AATGATGAAC 
CTGATCCCTG GTGGCGCAGT AGCAAAGCCA TTTATCACCT ATCACAATGA TCTGAACATG 
AACCTGTACA TGAGAATTGC TCCTGAGCTT TACCATAAGA TGTTGGTAGT TGGAGGAATC 
GACCGCGTGT ACGAGATTGG CCGTCAGTTC CGTAATGAAG GCATCGATCT CACTCACAAC 
CCAGAGTTCA CCACCTGTGA GTTCTACATG GCCTATGCCG ACTATCACGA CCTGATGGAA 
ATCACAGAGA AGCTTCTCTC AGGAATGGTG AAGCACATTA CCGGAGCTTA TAAAGTGACG 
TATCATCCTG ATGGTCCAGA GGGACAGGCC TACGAAATAG ACTTCACCCC TCCCTTCAGG 
CGAATCAGCA TGACCCAAGA GCTGGAGAAG GAGTTGGGTG TGAAGTTTCC ACCTCCTGAC 
ACCTATGACA GTGACGAAAT GCGCAAGTTC TTAGACGACC TGTGTGTTCA GAAAGAAGTT 
GAATGTCCTC CTCCCAGGAC AACTGCTCGA CTTCTTGATA AGTTGGTGGG AGACTTCCTG 
GAGGTGAAGT GCATCAACCC AACATACATC TGCGATCATC CACAGATAAT GAGTCCTCTT 
GCTAAATGGC ACAGGTCACA GAAAGGTCTA ACTGAACGTT TTGAACTCTT TGTGATGAAG 
AAGGAGATCT GCAATGCCTA CACAGAGTTG AACGATCCCA TCAGACAGCG AGAACTGTTT 
GAGCAGCAGG CTAAGGCTAA GGCAGAAGGT GACGACGAGG CCATGTTCAT TGATGAGACC 
TTCTGCACCG CTTTAGAGTA CGGTCTTCCC CCCACAGCAG GATGGGGAAT GGGCATCGAC 
CGCCTCACCA TGTTCCTCAC AGACTCCAAC AACATCAAGG AGGTGCTGCT TTTCCCTGCC 
ATGAAGCCAG AAGACAATAA ACCAGCTGCA CCTGCAGAGG GCACATCAGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases.
PloS one12(9)e0185317(2017)
Waldron AL,Cahan SH,Franklyn CS,Ebert AM

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)
Smith LC,Clark JC,Bisesi JH Jr,Ferguson PL,Sabo-Attwood T

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

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050-3174-7589(受注専用)

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kars1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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