irs2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	irs2
Entrez Gene ID	394823
Full Name	insulin receptor substrate 2
Synonyms	irs1,irsu
General protein information	Preferred Names insulin receptor substrate 2 Names insulin receptor substrate 1 IRS-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002942589.4	XP_002942635.3	insulin receptor substrate 1
XM_031899599.1	XP_031755459.1	insulin receptor substrate 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following irs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa41601	XM_002942589.4 Latest version!	Xenopus tropicalis insulin receptor substrate 2 (irs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa55554	XM_031899599.1 Latest version!	Xenopus tropicalis insulin receptor substrate 2 (irs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irs2 ( XM_002942589.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942635.3 Xenopus tropicalis insulin receptor substrate 2 (irs2), mRNA.

Price & Availability↑

CloneID	OXa41601
Clone ID Related Accession (Same CDS sequence)	XM_002942589.4
Accession Version	XM_002942589.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	insulin receptor substrate 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016688524.1) and transcript sequence (BC063198.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942589.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1636 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAGGACA TCAAGAAGTG CGGCTACCTG CGCAAGCAGA AATCCATGCG CAAAAGGTAC 
TTCGTCCTGC GCTGCCCCGG AACCCGCGGC CCGGCCCGCC TGGAATACTA CGAGAACGAG 
AAGAAGTTCC GAGTGGCGGA AGGATCGGGC GGCCCGCGGG GGGTGCTCAA CCTGGAGGAG 
GCGTTTGGCG TCAACAAGAG GTCAGATGCC AAGAAGCGGC ACCTACTGGT GATCTACACG 
CGGGACGGGG GGCTGGGCGT GTCCGCCGAC GGGGAGGAGG AGCAGGACGA GTGGTACCAA 
GCCATCCTGG AGGTGCAGGC ACAGGCCCGA GCACTCTCCT CATCTCCAGA TGCTCCTGCC 
TGGCCCGGCC CGGCATTCAG AGAAGTCTGG CAGGTCAGTG TCCGCCCACG AGGCCTGGGC 
CAAACGCGCA ATCTCTCCGG CATCTACCGG CTCTGCCTGG CAGAACGGAC CCTAGGCCTC 
CTCCGCTTAC GTTCTGAAAA TCCTTCCGTC ACCCTACAGC TCATGAATGT CCGGCGCTGC 
GGTCACTCCG ACAACTACTT CTTTGTGGAA GTCGGACGCT CGGCTGTGAC AGGTCCAGGA 
GAACTCTGGA TGCAGGTGGA AGACTCGGTG GTCGCTCAGA ACATGCACGA GACCATCCTT 
GAGGCGATGA AGTCTCTGAG TGAGGAATTT CGTCCGAGGA CCAAGAGCCA GTCCCTCTCA 
TCCACCCCCA TCTCTGTCCC ATCTCGACGT CACCACCCAA ACCCTCCTCC CCCGAGCCAA 
GTGGGCATTT CTCGTCGCTC GAGGGCCGAG ACGCCAATTG AAAACTCTCC GGTCCCCAAA 
CCCCACTCTT TGTCCAAGGA CTATAGTGTC CAATCGCCTG AAGAGGAGGA AGAGGAGAAA 
GGTGCACGGG TGGAAACCAA TGAGTCTTCA GCTGACTACG GCTCTGCTTC TTCTGATGAA 
TATGGTTCTA GTCCCGGTGT GTTGGAAGCC CCGGTGTTCC TACCTCCATC TCCTGGACCA 
AGAGAAACCA ATTACATCTC CATGGCGCTG TATGGGAGAA GATCACTGGT GATGGAGCCG 
ATTTCTACCA ATGCCAACGT GCCGGCGGAA GAGGGTAGTA GATTAACTCT ATTTCAGGAA 
GAAGATAATT ATGCCATGAT GGGCCAACGT GAGCCGCGCC AGGAGACTGG TTACATGCCC 
ATGCTGCCCG GCAGTAACAG GTCCCAGGAT TACATGCCCA TGACGCCCAC CAGCATCTCG 
CCACCTGCCC CCGTGGAAAT GGCCGGTTAT GTCATGATGT CACCCCTGGG CAGTTGTTCT 
CCGGAGATTG AGAGGTTGAG TTGGCCACCG TCCCAGGAGG TATCTGCTGG TAGTTCTGAT 
AGCCACGCCT CTGACTATAT GAACATGTGG TCACTGAGCC GCTCCGCCTC CAGCACCCCA 
CCCCCACAGG AGGCTTTTCT GTCCTCACCC GGCGGGCCCT GCAGGGTGCC GGCTTCATAC 
CGTTCTTTGC CTCGTTCATA CAAGATGGAG CCGCAGCCAT CTGCCCGTGC CTCTTGCTCC 
TCCTCCTCTG ACAGCTTGGA AGAGGTGAAT GCTGGGAAGA ACCGCAGACC TCTTAGCATC 
TCCATTGACT CGTGGAACAC AGGAACCTTG TCCGGCAACT ACCGTCGGCC CCCCAGTCCC 
GGAGAATACG TCAGCATCCA TTTCAGGGCC CCCCAGAAGA AGACCTCAGG GAAGGAAGCC 
ATCGATGCCC CAAGAGAGTC CGTTTCCATG GAGGAGCAGC CCTCGGCGAT GCCCAACGGG 
GACTACACGG AAATGGACTT TGTGCCTCAC AGAACTTTAC AACCTCATGT AAAAACGAAC 
GAGGAACGGG GAACGAACGG GGAAAATGCC TTCAAACCTG TGGGGAAATT ACTGCCGGCC 
TTGGAGAAGA CTTCCTCGGA GAAGGACTCC TGGGCTGTAG ATAAAGCCTT GTGGCCAACG 
ACTCTGAGAG AGGCCACAGG GATCCCCATA GAAAGTGATT TAGGGCGCCC CGAAAAGGTT 
CTGGCACCCA AGCCTTCGGC CGAAGAACCC GAACTGAAGA GCTTGGTGTC TGCGGTGACC 
GGCTGCAGCC TCAACATGGG GCAGGATCCC CCGATGCGGT TAGACCCCTC CACCCGGAAA 
AGGCACTGCT CCGAAAGCGT CCGTCCCCCC GCCCCCTGCA ACGGAACCCG GGCTGCGGCA 
CTCGAGCCCG GGCTGAACTA CATTGACTTG GATCTGTCCA AGGACGCAGC GGGCGGAGTT 
CCCCCGCCAG TGTTGGTTGG GCAAGGTGGG GCACCCCCCC CCAAGATTGG TTTTTTGGGA 
GGTCGGCCCA ACATCAACAC ATACGCCAGC ATGGATTTTC AGATGTGTGG GGAGCTGCGC 
CGGGGCAGCA AGGATGGGAC AGAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942635.3
CDS	141..2570
Translation	MEDIKKCGYLRKQKSMRKRYFVLRCPGTRGPARLEYYENEKKFRVAEGSGGPRGVLNLEEAFGVNKRSDAKKRHLLVIYTRDGGLGVSADGEEEQDEWYQAILEVQAQARALSSSPDAPAWPGPAFREVWQVSVRPRGLGQTRNLSGIYRLCLAERTLGLLRLRSENPSVTLQLMNVRRCGHSDNYFFVEVGRSAVTGPGELWMQVEDSVVAQNMHETILEAMKSLSEEFRPRTKSQSLSSTPISVPSRRHHPNPPPPSQVGISRRSRAETPIENSPVPKPHSLSKDYSVQSPEEEEEEKGARVETNESSADYGSASSDEYGSSPGVLEAPVFLPPSPGPRETNYISMALYGRRSLVMEPISTNANVPAEEGSRLTLFQEEDNYAMMGQREPRQETGYMPMLPGSNRSQDYMPMTPTSISPPAPVEMAGYVMMSPLGSCSPEIERLSWPPSQEVSAGSSDSHASDYMNMWSLSRSASSTPPPQEAFLSSPGGPCRVPASYRSLPRSYKMEPQPSARASCSSSSDSLEEVNAGKNRRPLSISIDSWNTGTLSGNYRRPPSPGEYVSIHFRAPQKKTSGKEAIDAPRESVSMEEQPSAMPNGDYTEMDFVPHRTLQPHVKTNEERGTNGENAFKPVGKLLPALEKTSSEKDSWAVDKALWPTTLREATGIPIESDLGRPEKVLAPKPSAEEPELKSLVSAVTGCSLNMGQDPPMRLDPSTRKRHCSESVRPPAPCNGTRAAALEPGLNYIDLDLSKDAAGGVPPPVLVGQGGAPPPKIGFLGGRPNINTYASMDFQMCGELRRGSKDGTEC

Target ORF information:

RefSeq Version	XM_002942589.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis insulin receptor substrate 2 (irs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942589.4

ORF Insert Sequence:

ATGGAGGACA TCAAGAAGTG CGGCTACCTG CGCAAGCAGA AATCCATGCG CAAAAGGTAC 
TTCGTCCTGC GCTGCCCCGG AACCCGCGGC CCGGCCCGCC TGGAATACTA CGAGAACGAG 
AAGAAGTTCC GAGTGGCGGA AGGATCGGGC GGCCCGCGGG GGGTGCTCAA CCTGGAGGAG 
GCGTTTGGCG TCAACAAGAG GTCAGATGCC AAGAAGCGGC ACCTACTGGT GATCTACACG 
CGGGACGGGG GGCTGGGCGT GTCCGCCGAC GGGGAGGAGG AGCAGGACGA GTGGTACCAA 
GCCATCCTGG AGGTGCAGGC ACAGGCCCGA GCACTCTCCT CATCTCCAGA TGCTCCTGCC 
TGGCCCGGCC CGGCATTCAG AGAAGTCTGG CAGGTCAGTG TCCGCCCACG AGGCCTGGGC 
CAAACGCGCA ATCTCTCCGG CATCTACCGG CTCTGCCTGG CAGAACGGAC CCTAGGCCTC 
CTCCGCTTAC GTTCTGAAAA TCCTTCCGTC ACCCTACAGC TCATGAATGT CCGGCGCTGC 
GGTCACTCCG ACAACTACTT CTTTGTGGAA GTCGGACGCT CGGCTGTGAC AGGTCCAGGA 
GAACTCTGGA TGCAGGTGGA AGACTCGGTG GTCGCTCAGA ACATGCACGA GACCATCCTT 
GAGGCGATGA AGTCTCTGAG TGAGGAATTT CGTCCGAGGA CCAAGAGCCA GTCCCTCTCA 
TCCACCCCCA TCTCTGTCCC ATCTCGACGT CACCACCCAA ACCCTCCTCC CCCGAGCCAA 
GTGGGCATTT CTCGTCGCTC GAGGGCCGAG ACGCCAATTG AAAACTCTCC GGTCCCCAAA 
CCCCACTCTT TGTCCAAGGA CTATAGTGTC CAATCGCCTG AAGAGGAGGA AGAGGAGAAA 
GGTGCACGGG TGGAAACCAA TGAGTCTTCA GCTGACTACG GCTCTGCTTC TTCTGATGAA 
TATGGTTCTA GTCCCGGTGT GTTGGAAGCC CCGGTGTTCC TACCTCCATC TCCTGGACCA 
AGAGAAACCA ATTACATCTC CATGGCGCTG TATGGGAGAA GATCACTGGT GATGGAGCCG 
ATTTCTACCA ATGCCAACGT GCCGGCGGAA GAGGGTAGTA GATTAACTCT ATTTCAGGAA 
GAAGATAATT ATGCCATGAT GGGCCAACGT GAGCCGCGCC AGGAGACTGG TTACATGCCC 
ATGCTGCCCG GCAGTAACAG GTCCCAGGAT TACATGCCCA TGACGCCCAC CAGCATCTCG 
CCACCTGCCC CCGTGGAAAT GGCCGGTTAT GTCATGATGT CACCCCTGGG CAGTTGTTCT 
CCGGAGATTG AGAGGTTGAG TTGGCCACCG TCCCAGGAGG TATCTGCTGG TAGTTCTGAT 
AGCCACGCCT CTGACTATAT GAACATGTGG TCACTGAGCC GCTCCGCCTC CAGCACCCCA 
CCCCCACAGG AGGCTTTTCT GTCCTCACCC GGCGGGCCCT GCAGGGTGCC GGCTTCATAC 
CGTTCTTTGC CTCGTTCATA CAAGATGGAG CCGCAGCCAT CTGCCCGTGC CTCTTGCTCC 
TCCTCCTCTG ACAGCTTGGA AGAGGTGAAT GCTGGGAAGA ACCGCAGACC TCTTAGCATC 
TCCATTGACT CGTGGAACAC AGGAACCTTG TCCGGCAACT ACCGTCGGCC CCCCAGTCCC 
GGAGAATACG TCAGCATCCA TTTCAGGGCC CCCCAGAAGA AGACCTCAGG GAAGGAAGCC 
ATCGATGCCC CAAGAGAGTC CGTTTCCATG GAGGAGCAGC CCTCGGCGAT GCCCAACGGG 
GACTACACGG AAATGGACTT TGTGCCTCAC AGAACTTTAC AACCTCATGT AAAAACGAAC 
GAGGAACGGG GAACGAACGG GGAAAATGCC TTCAAACCTG TGGGGAAATT ACTGCCGGCC 
TTGGAGAAGA CTTCCTCGGA GAAGGACTCC TGGGCTGTAG ATAAAGCCTT GTGGCCAACG 
ACTCTGAGAG AGGCCACAGG GATCCCCATA GAAAGTGATT TAGGGCGCCC CGAAAAGGTT 
CTGGCACCCA AGCCTTCGGC CGAAGAACCC GAACTGAAGA GCTTGGTGTC TGCGGTGACC 
GGCTGCAGCC TCAACATGGG GCAGGATCCC CCGATGCGGT TAGACCCCTC CACCCGGAAA 
AGGCACTGCT CCGAAAGCGT CCGTCCCCCC GCCCCCTGCA ACGGAACCCG GGCTGCGGCA 
CTCGAGCCCG GGCTGAACTA CATTGACTTG GATCTGTCCA AGGACGCAGC GGGCGGAGTT 
CCCCCGCCAG TGTTGGTTGG GCAAGGTGGG GCACCCCCCC CCAAGATTGG TTTTTTGGGA 
GGTCGGCCCA ACATCAACAC ATACGCCAGC ATGGATTTTC AGATGTGTGG GGAGCTGCGC 
CGGGGCAGCA AGGATGGGAC AGAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irs2 ( XM_031899599.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755459.1 Xenopus tropicalis insulin receptor substrate 2 (irs2), mRNA.

Price & Availability↑

CloneID	OXa55554
Clone ID Related Accession (Same CDS sequence)	XM_031899599.1
Accession Version	XM_031899599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	insulin receptor substrate 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA TCAAGAAGTG CGGCTACCTG CGCAAGCAGA AATCCATGCG CAAAAGGTAC 
TTCGTCCTGC GCTGCCCCGG AACCCGCGGC CCGGCCCGCC TGGAATACTA CGAGAACGAG 
AAGAAGTTCC GAGTGGCGGA AGGATCGGGC GGCCCGCGGG GGGTGCTCAA CCTGGAGGAG 
GCGTTTGGCG TCAACAAGAG GTCAGATGCC AAGAAGCGGC ACCTACTGGT GATCTACACG 
CGGGACGGGG GGCTGGGCGT GTCCGCCGAC GGGGAGGAGG AGCAGGACGA GTGGTACCAA 
GCCATCCTGG AGGTGCAGGC ACAGGCCCGA GCACTCTCCT CATCTCCAGA TGCTCCTGCC 
TGGCCCGGCC CGGCATTCAG AGAAGTCTGG CAGGTCAGTG TCCGCCCACG AGGCCTGGGC 
CAAACGCGCA ATCTCTCCGG CATCTACCGG CTCTGCCTGG CAGAACGGAC CCTAGGCCTC 
CTCCGCTTAC GTTCTGAAAA TCCTTCCGTC ACCCTACAGC TCATGAATGT CCGGCGCTGC 
GGTCACTCCG ACAACTACTT CTTTGTGGAA GTCGGACGCT CGGCTGTGAC AGGTCCAGGA 
GAACTCTGGA TGCAGGTGGA AGACTCGGTG GTCGCTCAGA ACATGCACGA GACCATCCTT 
GAGGCGATGA AGTCTCTGAG TGAGGAATTT CGTCCGAGGA CCAAGAGCCA GTCCCTCTCA 
TCCACCCCCA TCTCTGTCCC ATCTCGACGT CACCACCCAA ACCCTCCTCC CCCGAGCCAA 
GTGGGCATTT CTCGTCGCTC GAGGGCCGAG ACGCCAATTG AAAACTCTCC GGTCCCCAAA 
CCCCACTCTT TGTCCAAGGA CTATAGTGTC CAATCGCCTG AAGAGGAGGA AGAGGAGAAA 
GGTGCACGGG TGGAAACCAA TGAGTCTTCA GCTGACTACG GCTCTGCTTC TTCTGATGAA 
TATGGTTCTA GTCCCGGTGT GTTGGAAGCC CCGGTGTTCC TACCTCCATC TCCTGGACCA 
AGAGAAACCA ATTACATCTC CATGGCGCTG TATGGGAGAA GATCACTGGT GATGGAGCCG 
ATTTCTACCA ATGCCAACGT GCCGGCGGAA GAGGGTAGTA GATTAACTCT ATTTCAGGAA 
GAAGATAATT ATGCCATGAT GGGCCAACGT GAGCCGCGCC AGGAGACTGG TTACATGCCC 
ATGCTGCCCG GCAGTAACAG GTCCCAGGAT TACATGCCCA TGACGCCCAC CAGCATCTCG 
CCACCTGCCC CCGTGGAAAT GGCCGGTTAT GTCATGATGT CACCCCTGGG CAGTTGTTCT 
CCGGAGATTG AGAGGTTGAG TTGGCCACCG TCCCAGGAGG TATCTGCTGG TAGTTCTGAT 
AGCCACGCCT CTGACTATAT GAACATGTGG TCACTGAGCC GCTCCGCCTC CAGCACCCCA 
CCCCCACAGG AGGCTTTTCT GTCCTCACCC GGCGGGCCCT GCAGGGTGCC GGCTTCATAC 
CGTTCTTTGC CTCGTTCATA CAAGATGGAG CCGCAGCCAT CTGCCCGTGC CTCTTGCTCC 
TCCTCCTCTG ACAGCTTGGA AGAGGTGAAT GCTGGGAAGA ACCGCAGACC TCTTAGCATC 
TCCATTGACT CGTGGAACAC AGGAACCTTG TCCGGCAACT ACCGTCGGCC CCCCAGTCCC 
GGAGAATACG TCAGCATCCA TTTCAGGGCC CCCCAGAAGA AGACCTCAGG GAAGGAAGCC 
ATCGATGCCC CAAGAGAGTC CGTTTCCATG GAGGAGCAGC CCTCGGCGAT GCCCAACGGG 
GACTACACGG AAATGGACTT TGTGCCTCAC AGAACTTTAC AACCTCATGT AAAAACGAAC 
GAGGAACGGG GAACGAACGG GGAAAATGCC TTCAAACCTG TGGGGAAATT ACTGCCGGCC 
TTGGAGAAGA CTTCCTCGGA GAAGGACTCC TGGGCTGTAG ATAAAGCCTT GTGGCCAACG 
ACTCTGAGAG AGGCCACAGG GATCCCCATA GAAAGTGATT TAGGGCGCCC CGAAAAGGTT 
CTGGCACCCA AGCCTTCGGC CGAAGAACCC GAACTGAAGA GCTTGGTGTC TGCGGTGACC 
GGCTGCAGCC TCAACATGGG GCAGGATCCC CCGATGCGGT TAGACCCCTC CACCCGGAAA 
AGGCACTGCT CCGAAAGCGT CCGTCCCCCC GCCCCCTGCA ACGGAACCCG GGCTGCGGCA 
CTCGAGCCCG GGCTGAACTA CATTGACTTG GATCTGTCCA AGGACGCAGC GGGCGGAGTT 
CCCCCGGCAG TGTTGGTTGG GCAAGGTGGG GCACCGCCCC CCAAGATTGG TTCTCTGGGA 
GCTCCGCCCA ACATCAACAC CTACGCCAGC ATTGACTTTC AGATGTGTGG GGAGCTGCGC 
CGGGGCAGCA AGGATGGGAC AGAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755459.1
CDS	261..2690
Translation	MEDIKKCGYLRKQKSMRKRYFVLRCPGTRGPARLEYYENEKKFRVAEGSGGPRGVLNLEEAFGVNKRSDAKKRHLLVIYTRDGGLGVSADGEEEQDEWYQAILEVQAQARALSSSPDAPAWPGPAFREVWQVSVRPRGLGQTRNLSGIYRLCLAERTLGLLRLRSENPSVTLQLMNVRRCGHSDNYFFVEVGRSAVTGPGELWMQVEDSVVAQNMHETILEAMKSLSEEFRPRTKSQSLSSTPISVPSRRHHPNPPPPSQVGISRRSRAETPIENSPVPKPHSLSKDYSVQSPEEEEEEKGARVETNESSADYGSASSDEYGSSPGVLEAPVFLPPSPGPRETNYISMALYGRRSLVMEPISTNANVPAEEGSRLTLFQEEDNYAMMGQREPRQETGYMPMLPGSNRSQDYMPMTPTSISPPAPVEMAGYVMMSPLGSCSPEIERLSWPPSQEVSAGSSDSHASDYMNMWSLSRSASSTPPPQEAFLSSPGGPCRVPASYRSLPRSYKMEPQPSARASCSSSSDSLEEVNAGKNRRPLSISIDSWNTGTLSGNYRRPPSPGEYVSIHFRAPQKKTSGKEAIDAPRESVSMEEQPSAMPNGDYTEMDFVPHRTLQPHVKTNEERGTNGENAFKPVGKLLPALEKTSSEKDSWAVDKALWPTTLREATGIPIESDLGRPEKVLAPKPSAEEPELKSLVSAVTGCSLNMGQDPPMRLDPSTRKRHCSESVRPPAPCNGTRAAALEPGLNYIDLDLSKDAAGGVPPAVLVGQGGAPPPKIGSLGAPPNINTYASIDFQMCGELRRGSKDGTEC

Target ORF information:

RefSeq Version	XM_031899599.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis insulin receptor substrate 2 (irs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899599.1

ORF Insert Sequence:

ATGGAGGACA TCAAGAAGTG CGGCTACCTG CGCAAGCAGA AATCCATGCG CAAAAGGTAC 
TTCGTCCTGC GCTGCCCCGG AACCCGCGGC CCGGCCCGCC TGGAATACTA CGAGAACGAG 
AAGAAGTTCC GAGTGGCGGA AGGATCGGGC GGCCCGCGGG GGGTGCTCAA CCTGGAGGAG 
GCGTTTGGCG TCAACAAGAG GTCAGATGCC AAGAAGCGGC ACCTACTGGT GATCTACACG 
CGGGACGGGG GGCTGGGCGT GTCCGCCGAC GGGGAGGAGG AGCAGGACGA GTGGTACCAA 
GCCATCCTGG AGGTGCAGGC ACAGGCCCGA GCACTCTCCT CATCTCCAGA TGCTCCTGCC 
TGGCCCGGCC CGGCATTCAG AGAAGTCTGG CAGGTCAGTG TCCGCCCACG AGGCCTGGGC 
CAAACGCGCA ATCTCTCCGG CATCTACCGG CTCTGCCTGG CAGAACGGAC CCTAGGCCTC 
CTCCGCTTAC GTTCTGAAAA TCCTTCCGTC ACCCTACAGC TCATGAATGT CCGGCGCTGC 
GGTCACTCCG ACAACTACTT CTTTGTGGAA GTCGGACGCT CGGCTGTGAC AGGTCCAGGA 
GAACTCTGGA TGCAGGTGGA AGACTCGGTG GTCGCTCAGA ACATGCACGA GACCATCCTT 
GAGGCGATGA AGTCTCTGAG TGAGGAATTT CGTCCGAGGA CCAAGAGCCA GTCCCTCTCA 
TCCACCCCCA TCTCTGTCCC ATCTCGACGT CACCACCCAA ACCCTCCTCC CCCGAGCCAA 
GTGGGCATTT CTCGTCGCTC GAGGGCCGAG ACGCCAATTG AAAACTCTCC GGTCCCCAAA 
CCCCACTCTT TGTCCAAGGA CTATAGTGTC CAATCGCCTG AAGAGGAGGA AGAGGAGAAA 
GGTGCACGGG TGGAAACCAA TGAGTCTTCA GCTGACTACG GCTCTGCTTC TTCTGATGAA 
TATGGTTCTA GTCCCGGTGT GTTGGAAGCC CCGGTGTTCC TACCTCCATC TCCTGGACCA 
AGAGAAACCA ATTACATCTC CATGGCGCTG TATGGGAGAA GATCACTGGT GATGGAGCCG 
ATTTCTACCA ATGCCAACGT GCCGGCGGAA GAGGGTAGTA GATTAACTCT ATTTCAGGAA 
GAAGATAATT ATGCCATGAT GGGCCAACGT GAGCCGCGCC AGGAGACTGG TTACATGCCC 
ATGCTGCCCG GCAGTAACAG GTCCCAGGAT TACATGCCCA TGACGCCCAC CAGCATCTCG 
CCACCTGCCC CCGTGGAAAT GGCCGGTTAT GTCATGATGT CACCCCTGGG CAGTTGTTCT 
CCGGAGATTG AGAGGTTGAG TTGGCCACCG TCCCAGGAGG TATCTGCTGG TAGTTCTGAT 
AGCCACGCCT CTGACTATAT GAACATGTGG TCACTGAGCC GCTCCGCCTC CAGCACCCCA 
CCCCCACAGG AGGCTTTTCT GTCCTCACCC GGCGGGCCCT GCAGGGTGCC GGCTTCATAC 
CGTTCTTTGC CTCGTTCATA CAAGATGGAG CCGCAGCCAT CTGCCCGTGC CTCTTGCTCC 
TCCTCCTCTG ACAGCTTGGA AGAGGTGAAT GCTGGGAAGA ACCGCAGACC TCTTAGCATC 
TCCATTGACT CGTGGAACAC AGGAACCTTG TCCGGCAACT ACCGTCGGCC CCCCAGTCCC 
GGAGAATACG TCAGCATCCA TTTCAGGGCC CCCCAGAAGA AGACCTCAGG GAAGGAAGCC 
ATCGATGCCC CAAGAGAGTC CGTTTCCATG GAGGAGCAGC CCTCGGCGAT GCCCAACGGG 
GACTACACGG AAATGGACTT TGTGCCTCAC AGAACTTTAC AACCTCATGT AAAAACGAAC 
GAGGAACGGG GAACGAACGG GGAAAATGCC TTCAAACCTG TGGGGAAATT ACTGCCGGCC 
TTGGAGAAGA CTTCCTCGGA GAAGGACTCC TGGGCTGTAG ATAAAGCCTT GTGGCCAACG 
ACTCTGAGAG AGGCCACAGG GATCCCCATA GAAAGTGATT TAGGGCGCCC CGAAAAGGTT 
CTGGCACCCA AGCCTTCGGC CGAAGAACCC GAACTGAAGA GCTTGGTGTC TGCGGTGACC 
GGCTGCAGCC TCAACATGGG GCAGGATCCC CCGATGCGGT TAGACCCCTC CACCCGGAAA 
AGGCACTGCT CCGAAAGCGT CCGTCCCCCC GCCCCCTGCA ACGGAACCCG GGCTGCGGCA 
CTCGAGCCCG GGCTGAACTA CATTGACTTG GATCTGTCCA AGGACGCAGC GGGCGGAGTT 
CCCCCGGCAG TGTTGGTTGG GCAAGGTGGG GCACCGCCCC CCAAGATTGG TTCTCTGGGA 
GCTCCGCCCA ACATCAACAC CTACGCCAGC ATTGACTTTC AGATGTGTGG GGAGCTGCGC 
CGGGGCAGCA AGGATGGGAC AGAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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irs2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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