spg11 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	spg11
Entrez Gene ID	394666
Full Name	SPG11, spatacsin vesicle trafficking associated
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names spatacsin spastic paraplegia 11 (autosomal recessive)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012958756.1	XP_012814210.1	spatacsin isoform X1
XM_031897783.1	XP_031753643.1	spatacsin isoform X2
XM_012958758.2	XP_012814212.2	spatacsin isoform X1
NM_203738.2	NP_989069.1	spatacsin
NM_203738.3	NP_989069.2	spatacsin
XM_012958758.1	XP_012814212.1	spatacsin isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPG11	XP_510642.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008242	XP_001689012.1
Xenopus tropicalis (tropical clawed frog)	spg11	NP_989069.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spg11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spg11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21295	NM_203738.2 Latest version!	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OXa25973	NM_203738.3 Latest version!	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa53409	XM_012958758.2 Latest version!	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa53410	XM_031897783.1 Latest version!	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa25973	XM_012958756.1 Latest version!	Xenopus tropicalis spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OXa25974	XM_012958758.1 Latest version!	Xenopus tropicalis spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spg11 ( NM_203738.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_989069.1 Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), mRNA.

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CloneID	OXa21295
Clone ID Related Accession (Same CDS sequence)	NM_203738.2
Accession Version	NM_203738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-03
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spatacsin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061290.1. On Aug 31, 2012 this sequence version replaced NM_203738.1. ##Evidence-Data-START## Transcript exon combination :: BC061290.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

ATGCCCCCTG TACCCAGTGA TCCTGTGCTC TTAGATCTGG AAATTCTGAA GGACAGGTGC 
TCCCATGGCA AAACCTACTG CCAACAGCTG CTGTGTCTTT ACGAGTTAAG CCAGGACCTG 
GGATGTTCCT TCTCAGATAT CTCCTCTCGA GATCCAGGCG AGATTCTACG CTCTCTGCTT 
TCTTCTCAGC GTCCCGAGCT CACAGACAGA GCACAAGCAG TCATCAACTT CCACGGACTG 
TCGCCCCAAA CAGTGGCACA GATTGTGGCA GAAGAGGGTG TGAGAGTCTG GCGGGCAGGG 
CCCGTGGAAG TTTATAATGC CTCTGAAATA AGGCAGCGCT TTTTGCAGCT TGTAAAACTT 
TGCCAGGACC CAACTCTGGT TGGATTAACG CTACTTGACT ATCTAGAAAA TGTGCCACTG 
ACTGAGCAAC ACTGCATTAT TGAACTCCTG ATTTCTGCCC ACGATTGCTT CAGTTTAACG 
TGCCATCTTG AAGGAATTCG ACGGGTGCTT CAGGCATGTC GACACCTAAC CGAGAGTCAC 
TTGGCGGCTA ACCAAGAATA CAGCCTTATG GTTCGCCTAC TCTCTGGGAT TGGTCGTTAT 
AATGAGATGG TTTATGTGTT TGACATTCTG CACAAGGAAC AGCACTTTGA GGTTCTGCTG 
AGGAAACAGC TAGATACAAA GGGGGGACTG CAGACTGCTC TTTTGGAATA CATTAAGCGC 
TGCCATCCAG GAGACAGCGA GAAGCACAAC ATGACTGCGC TCTGCTTCAG TTTGCACCGT 
GACATTGGCC ACAACCATGA GCAGGCAGCT TTGATCCAGC TCAAACTCAT CCAGTCTAGG 
GCATGGGAAT ACTGGATGTC TGAATTGGTG GAGTTGAGAT CTGCACTAAT GAAAGCACTT 
ACTCTCCTAA TAGATGCAGC TGAGAGCTAC TCAAAGGATT CCTGCGTCCG TCAGTCTCTG 
CGCTGTGCTC GCCTTACCCG TCTGTTGACT CTGCAGCTTC ATCTGCTAAA CAACAGTCAT 
CAAACTAAAT TAATCAACCT GGATAGAGAG AACTTGATGG AGCCAATACT GGAACTGCCA 
CGTTTCTACC AGGCAGTCAT TGTTACAGAG GCCTATGATA TACAGCCAGA CTGGGCAGAG 
GTTTTATATC ACAAGGTGAT TCTTGCTGGA GACTTTCAGT ATTTAAATGA ATTCAAGCAG 
CGAGAACTGC TACGGAGTGG AGTGTTCCAG CAACTGTCTG ACAAGTGTAA ATTACAGCCT 
CCAGGAGCTA CCGGACTTCA AAATCTGAAG AGAGTGCTCG GTTACTGCGA AGATACCTAT 
ACCCGCTACA AACTGGCCTT GGAAAATCAG TTCTATGATG TGACCGATGT TTTAATGAGA 
GACTCACAAA CCCGCTGCTG CCTGACTGAC ATGCTGTCTC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989069.1
CDS	70..1494
Translation	MPPVPSDPVLLDLEILKDRCSHGKTYCQQLLCLYELSQDLGCSFSDISSRDPGEILRSLLSSQRPELTDRAQAVINFHGLSPQTVAQIVAEEGVRVWRAGPVEVYNASEIRQRFLQLVKLCQDPTLVGLTLLDYLENVPLTEQHCIIELLISAHDCFSLTCHLEGIRRVLQACRHLTESHLAANQEYSLMVRLLSGIGRYNEMVYVFDILHKEQHFEVLLRKQLDTKGGLQTALLEYIKRCHPGDSEKHNMTALCFSLHRDIGHNHEQAALIQLKLIQSRAWEYWMSELVELRSALMKALTLLIDAAESYSKDSCVRQSLRCARLTRLLTLQLHLLNNSHQTKLINLDRENLMEPILELPRFYQAVIVTEAYDIQPDWAEVLYHKVILAGDFQYLNEFKQRELLRSGVFQQLSDKCKLQPPGATGLQNLKRVLGYCEDTYTRYKLALENQFYDVTDVLMRDSQTRCCLTDMLSR

Target ORF information:

RefSeq Version	NM_203738.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203738.2

ORF Insert Sequence:

ATGCCCCCTG TACCCAGTGA TCCTGTGCTC TTAGATCTGG AAATTCTGAA GGACAGGTGC 
TCCCATGGCA AAACCTACTG CCAACAGCTG CTGTGTCTTT ACGAGTTAAG CCAGGACCTG 
GGATGTTCCT TCTCAGATAT CTCCTCTCGA GATCCAGGCG AGATTCTACG CTCTCTGCTT 
TCTTCTCAGC GTCCCGAGCT CACAGACAGA GCACAAGCAG TCATCAACTT CCACGGACTG 
TCGCCCCAAA CAGTGGCACA GATTGTGGCA GAAGAGGGTG TGAGAGTCTG GCGGGCAGGG 
CCCGTGGAAG TTTATAATGC CTCTGAAATA AGGCAGCGCT TTTTGCAGCT TGTAAAACTT 
TGCCAGGACC CAACTCTGGT TGGATTAACG CTACTTGACT ATCTAGAAAA TGTGCCACTG 
ACTGAGCAAC ACTGCATTAT TGAACTCCTG ATTTCTGCCC ACGATTGCTT CAGTTTAACG 
TGCCATCTTG AAGGAATTCG ACGGGTGCTT CAGGCATGTC GACACCTAAC CGAGAGTCAC 
TTGGCGGCTA ACCAAGAATA CAGCCTTATG GTTCGCCTAC TCTCTGGGAT TGGTCGTTAT 
AATGAGATGG TTTATGTGTT TGACATTCTG CACAAGGAAC AGCACTTTGA GGTTCTGCTG 
AGGAAACAGC TAGATACAAA GGGGGGACTG CAGACTGCTC TTTTGGAATA CATTAAGCGC 
TGCCATCCAG GAGACAGCGA GAAGCACAAC ATGACTGCGC TCTGCTTCAG TTTGCACCGT 
GACATTGGCC ACAACCATGA GCAGGCAGCT TTGATCCAGC TCAAACTCAT CCAGTCTAGG 
GCATGGGAAT ACTGGATGTC TGAATTGGTG GAGTTGAGAT CTGCACTAAT GAAAGCACTT 
ACTCTCCTAA TAGATGCAGC TGAGAGCTAC TCAAAGGATT CCTGCGTCCG TCAGTCTCTG 
CGCTGTGCTC GCCTTACCCG TCTGTTGACT CTGCAGCTTC ATCTGCTAAA CAACAGTCAT 
CAAACTAAAT TAATCAACCT GGATAGAGAG AACTTGATGG AGCCAATACT GGAACTGCCA 
CGTTTCTACC AGGCAGTCAT TGTTACAGAG GCCTATGATA TACAGCCAGA CTGGGCAGAG 
GTTTTATATC ACAAGGTGAT TCTTGCTGGA GACTTTCAGT ATTTAAATGA ATTCAAGCAG 
CGAGAACTGC TACGGAGTGG AGTGTTCCAG CAACTGTCTG ACAAGTGTAA ATTACAGCCT 
CCAGGAGCTA CCGGACTTCA AAATCTGAAG AGAGTGCTCG GTTACTGCGA AGATACCTAT 
ACCCGCTACA AACTGGCCTT GGAAAATCAG TTCTATGATG TGACCGATGT TTTAATGAGA 
GACTCACAAA CCCGCTGCTG CCTGACTGAC ATGCTGTCTC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( NM_203738.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_989069.2 Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), mRNA.

Price & Availability↑

CloneID	OXa25973
Clone ID Related Accession (Same CDS sequence)	XM_012958756.1 , NM_203738.3
Accession Version	NM_203738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spatacsin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03012792.1. On or before Oct 7, 2019 this sequence version replaced XM_012958756.1, NM_203738.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGACTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGTAGATA TGGGTTTCTC ACACCCAAGA 
AATCTGTGGT GTCATCTTAC TTTATGGCAT GCATGGCAGA GGATTTCTTC ATGGATAGAA 
TCTTTAGAAA AACATGGAAA GACAAGTAAC AATGATCTCT CAACATGGCC TCTTCTGACT 
CCAGAAATAG TGGAGTCATG CACCCTCTGT TGTGACTATA CAAAGCAGAA GATCCTGGAC 
AAGTTAGCAA GAATGGAAAT ATTTGTCCCT TCTGAGCAGC AGAATTTTGA GGCCCTTGTG 
GAACGTATGG CAGTTAGTAG GAGCATGATG CCGTTTCCTG TCTCCAACCC TCAGTGCAGC 
ACCTCTGAGA ATCAAGATCG TCACACCCAC TTCGTTTTGC TGTGTGCTGA GCAGAGTCTT 
CACTACCTAT TGTACACATA TCTGGATTAT CACAGGTTGA ATTCTCAGCT TTGCTCTGCT 
TTGGGCAATA CAACCTTGCA TGAAACCTTG CCTTGGTTTG CATTTTTGGT ACAGATACGA 
GGTGTCAAAG ACAACTCTGA AGATCCAAGC CGCATGTTTA ATGCTAGCCT CTCCAATGCC 
CATATGGTGA TGCCAGGGTA CCAGCCTAGT ATGAGCAGCA TGCTGCTGGA GGGACACACA 
TTGCTCGCCC TTGCAACCAA TATGTATGCA CCTGGTGGCA TTGACAGGGT GTTGGAGCAG 
CATGGAGAGT CAGATTCTGG TCATAGAAAT GTAGACCCTC AGCTTCTTAA AATGGCTTTA 
GCTCCATATC CGAAGCTCAA GGCAGCTCTC TTCAGTACAC ACCCAGCTAC TGTCAGTTCT 
CCCTCTGACA TTTCACTTTA CCATCTCCTA CAGGCTCTCT CCCCATTTCA GCCTACACAC 
TTCTTCACAT GGCAGTCGGT AAATACCCTT GCTGCAAGTG ACAACAGTGC AGTGCTCCCA 
CATTGCTCAA GTCCAGCCTT GGTCAGCAAG TTCTCTGTGG GTGAGCAACT AGATGTCTTC 
TTCTTCCTGC GGAGCGGGCG ACCATCAGTC GCATTCGCTA CCTTTCTGGT GTTTCAGCTA 
CTAAGAAGCA AAACCCCCCA ACAGCTAATC AAGCAAGTTG CTGAGGATGT TTATTCCCTG 
GCACTGTCTT CATTTCATCT CTCCTCTGTG CTTGCTTCAT GCTGCTGCTT CTTTGAGTTA 
CTGGGTCTCA ACAGCCACAA GCTTCGAGTG GATGTGAACG TTGCACACAT GATACTAAGT 
CACTCAGCTT CTCTGCATGA GGATCCAGGG GTACACAAAT CCCAGGCACA AGCTTTAGCT 
CACAGACTTT GCAGACTGGT GGAGAATGAG AGAGAAGCAG CCATGGATAT TTTACATAGC 
CTGGAGGAAG CTGTATTTTC TGAAGAGTGT CAAAACAGCT CATCTCTACC CACTGGCTCC 
TGTTTAACTA TTCAGCAGTT CTGCCTCCTC CATTCTATTC CCATGAGCAC TAGGTATTTG 
AGGAGCTGCG CACAGCAACA AGACTGGCTG CAGTTACTTG TGAACACACA CACTCAGGAA 
CAGGTCCTAT GTGTAGCTGA AAAGTTGAGC CCGGCACTTA ATAATCACAT AATGCTATCA 
TTGCAAGATG CAAATTCAGA TGCACAAATC TCTGAATATG ACTTGGAAAG ATCTGAAAAT 
GATACAGTGA CTGCCAGTTT GTACAATATA TTGCTTGGGT GCCAGAAGAC TGGGCAGCCA 
GGCCGCACAC TTCTAAAGGA ATGTATTAAG CACAGAGCTC CATTGCTGAG TGTGCTTGCT 
GCATATATGC AGGACACCAA CCAAATTGCT TGCCTGTGTG TGTGGCTTGT AACCTCTGTA 
GATCCGGCCA ATTGTGCAGA ACTCACAAAT ATGCTTTCGG TTGCTTCAGA TCATGAGTGG 
GACTTGCAAG ATCTGACACG GATTTGGGAA ATCTTGCTGG AGAGAAAAGA TAGCCAGACT 
TTGTACAAAG CATTCAGCAT CTTTTTAGAG GACTGTCCCC TTCTTCTCTT ACTTGAACTG 
TATGAGCTTT GCCTGCAACA TAAATACTAT CTGGAGGCTG GACAGAAAAT AGATGATTTC 
CAAAGATGGA TGATGAACGA TGACACTGAA GATACAGAGT TATCGCTTCT CATTCCACTG 
CCCTGGTTGC TACAAAGAGC TTCTCAACTT CTGCAGCTCC TTCTCTTGCA GACCCAGACT 
CCATATGAAT TGCGGAAGGT TTTGCAGCTT CTGAGTGACA AAGCTAAACC ACAGTTATTT 
GGCGGAATAG ATGTTCATAA GCTGAGTGAT CTAACCTCTA TTCTTCAAGA CCACCCTATT 
GCCATTAGCA AAGAATTAAT ATGCCACTAT AGTCCTGAAG CCCTGCAGGT TGAGTGTCAA 
AGGCTTATAC AGATGCTGTT GGACAAAAGG ATTTTTTCTC TTGCACAAAG AGTAGCAGAA 
CTAGCAGAAT TGCCCACTGA CAGTATTGTC ATAGAAGAGG TGTTGCAGGA CCAATGTCTC 
TTAAAAGAGG TTGGGCAGTG GGAGAATTCT CAAAGCCGTG TACAGTACTG GAGAAAATGC 
CACCAGGTGT TTAGCGCCAC GGAGCTCTCC CCAGCTGCAG CGTCTGACTT CTTCTGCTCT 
CAGACTCTTT CTCTTGTAGC GCCTGAATGC TTGCTTAGGG ATCAGACAGA GTTTTTGGCA 
GAGCAGGAGA TGCTGTTTAC CCTTGCTGGG CACTGGAGGT CCCTAAGTAA CACTGCTTCT 
ATAGAAGTCC TGGAGGAAAT TGAGCAGAAG ATTTGGAAAT GTCGCCTGGC AAGAGAAGTA 
TTGAGTAAAC ATGGAGCTGC TAGTCAACTC TGTACTCTGC CTTCATTTGC TAGCTTGACC 
CTGGAGTTTT CATTCTCATC TTTGCCTGCA CTAAACTCTC CTTCGCTTCT TCATATCTCT 
AATTTGCCAC CGCTTGGTAC TCCTAGCAGT TTGTTGGACA GAGAACAGAC CAAAGCATTG 
AGCTCTTTGG TTGACTGCCT GTTGGATGAG AGCCGTGTGC ACGAGGCCAG TCGGGTATGC 
AGGTACTTTC TGCTACCTCA TTATGATCTT TGGCTACTGC TGAACTGCAG GGCACTAGCA 
ACAGGAGAGA CCGTAAGGGA CCAACTGCAT CCGGAAATTC AAGCTATCCT CGGTGAAGGC 
AGCGAACGCC GGGAAAGCAT ATGGCACCAG AGGAAAAGAC TGCACAGCTC CTCCTCTCTG 
GACACCGCCA TGCCCCCTGT ACCCAGTGAT CCTGTGCTCT TAGATCTGGA AATTCTGAAG 
GACAGGTGCT CCCATGGCAA AACCTACTGC CAACAGCTGC TGTGTCTTTA CGAGTTAAGC 
CAGGACCTGG GATGTTCCTT CTCAGATATC TCCTCTCGAG ATCCCGGCGA GATTCTACGC 
TCTCTGCTTT CTTCTCAGCG TCCCGAGCTC ACAGACAGAG CACAAGCAGT CATCAACTTC 
CACGGACTGT CGCCGCAAAC AGTGGCACAG ATTGTGGCAG AAGAGGGTGT GAGAGTCTGG 
CGGGCAGGGC CCGTGGAAGT TTATAATGCC TCTGAAATAA GGCAGCGCTT TTTGCAGCTT 
GTAAAACTTT GCCAGGACCC AACTCTGGTT GGATTAACGC TACTTGACTA TCTAGAAAAT 
GTGCCACTGA CTGAGCAACA CTGCATTATT GAACTCCTGA TTTCTGCCCA CGATTGCTTC 
AGTTTAACGT GCCATCTTGA AGGAATTCGA CGGGTGCTTC AGGCATGTCG ACACCTAACC 
GAGAGTCACT TGGCGGCTAA CCAAGAATAC AGCCTTATGG TTCGCCTACT CTCTGGGATT 
GGTCGTTATA ATGAGATGGT TTATGTGTTT GACATTCTGC ACAAGGAACA GCACTTTGAG 
GTTCTGCTGA GGAAACAGCT AGATACAAAG GGGGGACTGC AGACTGCTCT TTTGGAATAC 
ATTAAGCGCT GCCATCCAGG AGACAGCGAG AAGCACAACA TGACTGCGCT CTGCTTCAGT 
TTGCACCGTG ACATTGGCCA CAACCATGAG CAGGCAGCTT TGATCCAGCT CAAACTCATC 
CAGTCTAGGG CATGGGAATA CTGGATGTCT GAATTGGTGG AGTTGAGATC TGCACTAATG 
AAAGCACTTA CCCTCCTAAT AGATGCAGCT GAGAGCTACT CAAAGGATTC CTGCGTCCGT 
CAGTCTCTGC GCTGTGCTCG CCTTACCCGT CTGTTGACTC TGCAGCTTCA TCTGCTAAAC 
AACAGTCATC AAACTAAATT AATCAACCTG GATAGAGAGA ACTTGATGGA GCCAATACTG 
GAACTGCCAC GTTTCTACCA GGCAGTCATT GTTACAGAGG CCTATGATAT ACAGCCAGAC 
TGGGCAGAGG TTTTATATCA CAAGGTGATT CTTGCTGGAG ACTTTCAGTA TTTAAATGAA 
TTCAAGCAGC GAGAACTGCT ACGGAGTGGA GTGTTCCAGC AACTGTCTGA CAAGTGTAAA 
TTACAGCCTC CAGGAGCTAC CGGACTTCAA AATCTGAAGA GAGTGCTCGG TTACTGCGAA 
GATACCTATA CCCGCTACAA ACTGGCCTTG GAAAACCAGT TCTATGATGT GACCGATGTT 
TTAATGAGAG ACTCACAAAC CCGCTGCTGC CTGACTGACA TGCTGTCTCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989069.2
CDS	215..7288
Translation	MGRRILQSCKMNLNILLVPGLTLPEGTNPPVRVSLTRQKHPTLCSLCPQGELHLTALPYEGFHTTHTQLEGLFSHFLWENADGNEEPSDNIPRLLTLSGSNEVAVHGLHVADGHLNVTTIFLCDEDTLRKLCADNHVCVSSVICVRLLSLQGDDVLLLLSSSVLLHLHFSSEDNSLKLLSCLCLDLGFEATERISDVCLKSGLLFMLDTYGFVYVYDTVDGSPIFTMELPLRPENAALDPLNSICVSDDLGLFVVTTAASWALSVQLCEDAREISDHAVSAWHSDHVPDLHISGADEERLSSCHESVCTPRCTLRSDRSWDATLSSLCNEIMNSSDTFVCSKNPYLWPCNLPLKVPLSGIPVGEGWVRIVSEGLDDPLSLHIGSVTSFSAVLCLFGMDGLLNMIQWDMQSKEISSYPLGKAHFVHCSQEDACLVMSDRGLSLVVFSASQEEFLTRLMIHGSASTADTLCLLNKWGRCSVPIHTLEAGLDNRQLDTVDFFLKSKVSILNPLISEEPPDGIQAERYLQNVQDLLPALDLLVSYIRETDQETQSKHFSEQLLQLTLGFLNGQLHLLCALLSESSPALQQAVEILMGYITKLRRFMKKFMEQPQPVDVPGIEGQLHSWKNLSLEQIISDAILTNRIPEAQVFLRAEGHQACSLTWLKQEGLNLVYKCLLETRLQETRQLLRNMGFSVMNELKRICLHTSDRGVRTLLVDLLQREGCFSDQEQEDIQTVIHLEEVYCCPQKRTKESHKLLPHRLVQRLFTGSQEPEVLQAALTPSKNKSHMVLLYWAQGWDLETKEAILLPLRNSQVDMGFSHPRNLWCHLTLWHAWQRISSWIESLEKHGKTSNNDLSTWPLLTPEIVESCTLCCDYTKQKILDKLARMEIFVPSEQQNFEALVERMAVSRSMMPFPVSNPQCSTSENQDRHTHFVLLCAEQSLHYLLYTYLDYHRLNSQLCSALGNTTLHETLPWFAFLVQIRGVKDNSEDPSRMFNASLSNAHMVMPGYQPSMSSMLLEGHTLLALATNMYAPGGIDRVLEQHGESDSGHRNVDPQLLKMALAPYPKLKAALFSTHPATVSSPSDISLYHLLQALSPFQPTHFFTWQSVNTLAASDNSAVLPHCSSPALVSKFSVGEQLDVFFFLRSGRPSVAFATFLVFQLLRSKTPQQLIKQVAEDVYSLALSSFHLSSVLASCCCFFELLGLNSHKLRVDVNVAHMILSHSASLHEDPGVHKSQAQALAHRLCRLVENEREAAMDILHSLEEAVFSEECQNSSSLPTGSCLTIQQFCLLHSIPMSTRYLRSCAQQQDWLQLLVNTHTQEQVLCVAEKLSPALNNHIMLSLQDANSDAQISEYDLERSENDTVTASLYNILLGCQKTGQPGRTLLKECIKHRAPLLSVLAAYMQDTNQIACLCVWLVTSVDPANCAELTNMLSVASDHEWDLQDLTRIWEILLERKDSQTLYKAFSIFLEDCPLLLLLELYELCLQHKYYLEAGQKIDDFQRWMMNDDTEDTELSLLIPLPWLLQRASQLLQLLLLQTQTPYELRKVLQLLSDKAKPQLFGGIDVHKLSDLTSILQDHPIAISKELICHYSPEALQVECQRLIQMLLDKRIFSLAQRVAELAELPTDSIVIEEVLQDQCLLKEVGQWENSQSRVQYWRKCHQVFSATELSPAAASDFFCSQTLSLVAPECLLRDQTEFLAEQEMLFTLAGHWRSLSNTASIEVLEEIEQKIWKCRLAREVLSKHGAASQLCTLPSFASLTLEFSFSSLPALNSPSLLHISNLPPLGTPSSLLDREQTKALSSLVDCLLDESRVHEASRVCRYFLLPHYDLWLLLNCRALATGETVRDQLHPEIQAILGEGSERRESIWHQRKRLHSSSSLDTAMPPVPSDPVLLDLEILKDRCSHGKTYCQQLLCLYELSQDLGCSFSDISSRDPGEILRSLLSSQRPELTDRAQAVINFHGLSPQTVAQIVAEEGVRVWRAGPVEVYNASEIRQRFLQLVKLCQDPTLVGLTLLDYLENVPLTEQHCIIELLISAHDCFSLTCHLEGIRRVLQACRHLTESHLAANQEYSLMVRLLSGIGRYNEMVYVFDILHKEQHFEVLLRKQLDTKGGLQTALLEYIKRCHPGDSEKHNMTALCFSLHRDIGHNHEQAALIQLKLIQSRAWEYWMSELVELRSALMKALTLLIDAAESYSKDSCVRQSLRCARLTRLLTLQLHLLNNSHQTKLINLDRENLMEPILELPRFYQAVIVTEAYDIQPDWAEVLYHKVILAGDFQYLNEFKQRELLRSGVFQQLSDKCKLQPPGATGLQNLKRVLGYCEDTYTRYKLALENQFYDVTDVLMRDSQTRCCLTDMLSR

Target ORF information:

RefSeq Version	NM_203738.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203738.3

ORF Insert Sequence:

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGACTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGTAGATA TGGGTTTCTC ACACCCAAGA 
AATCTGTGGT GTCATCTTAC TTTATGGCAT GCATGGCAGA GGATTTCTTC ATGGATAGAA 
TCTTTAGAAA AACATGGAAA GACAAGTAAC AATGATCTCT CAACATGGCC TCTTCTGACT 
CCAGAAATAG TGGAGTCATG CACCCTCTGT TGTGACTATA CAAAGCAGAA GATCCTGGAC 
AAGTTAGCAA GAATGGAAAT ATTTGTCCCT TCTGAGCAGC AGAATTTTGA GGCCCTTGTG 
GAACGTATGG CAGTTAGTAG GAGCATGATG CCGTTTCCTG TCTCCAACCC TCAGTGCAGC 
ACCTCTGAGA ATCAAGATCG TCACACCCAC TTCGTTTTGC TGTGTGCTGA GCAGAGTCTT 
CACTACCTAT TGTACACATA TCTGGATTAT CACAGGTTGA ATTCTCAGCT TTGCTCTGCT 
TTGGGCAATA CAACCTTGCA TGAAACCTTG CCTTGGTTTG CATTTTTGGT ACAGATACGA 
GGTGTCAAAG ACAACTCTGA AGATCCAAGC CGCATGTTTA ATGCTAGCCT CTCCAATGCC 
CATATGGTGA TGCCAGGGTA CCAGCCTAGT ATGAGCAGCA TGCTGCTGGA GGGACACACA 
TTGCTCGCCC TTGCAACCAA TATGTATGCA CCTGGTGGCA TTGACAGGGT GTTGGAGCAG 
CATGGAGAGT CAGATTCTGG TCATAGAAAT GTAGACCCTC AGCTTCTTAA AATGGCTTTA 
GCTCCATATC CGAAGCTCAA GGCAGCTCTC TTCAGTACAC ACCCAGCTAC TGTCAGTTCT 
CCCTCTGACA TTTCACTTTA CCATCTCCTA CAGGCTCTCT CCCCATTTCA GCCTACACAC 
TTCTTCACAT GGCAGTCGGT AAATACCCTT GCTGCAAGTG ACAACAGTGC AGTGCTCCCA 
CATTGCTCAA GTCCAGCCTT GGTCAGCAAG TTCTCTGTGG GTGAGCAACT AGATGTCTTC 
TTCTTCCTGC GGAGCGGGCG ACCATCAGTC GCATTCGCTA CCTTTCTGGT GTTTCAGCTA 
CTAAGAAGCA AAACCCCCCA ACAGCTAATC AAGCAAGTTG CTGAGGATGT TTATTCCCTG 
GCACTGTCTT CATTTCATCT CTCCTCTGTG CTTGCTTCAT GCTGCTGCTT CTTTGAGTTA 
CTGGGTCTCA ACAGCCACAA GCTTCGAGTG GATGTGAACG TTGCACACAT GATACTAAGT 
CACTCAGCTT CTCTGCATGA GGATCCAGGG GTACACAAAT CCCAGGCACA AGCTTTAGCT 
CACAGACTTT GCAGACTGGT GGAGAATGAG AGAGAAGCAG CCATGGATAT TTTACATAGC 
CTGGAGGAAG CTGTATTTTC TGAAGAGTGT CAAAACAGCT CATCTCTACC CACTGGCTCC 
TGTTTAACTA TTCAGCAGTT CTGCCTCCTC CATTCTATTC CCATGAGCAC TAGGTATTTG 
AGGAGCTGCG CACAGCAACA AGACTGGCTG CAGTTACTTG TGAACACACA CACTCAGGAA 
CAGGTCCTAT GTGTAGCTGA AAAGTTGAGC CCGGCACTTA ATAATCACAT AATGCTATCA 
TTGCAAGATG CAAATTCAGA TGCACAAATC TCTGAATATG ACTTGGAAAG ATCTGAAAAT 
GATACAGTGA CTGCCAGTTT GTACAATATA TTGCTTGGGT GCCAGAAGAC TGGGCAGCCA 
GGCCGCACAC TTCTAAAGGA ATGTATTAAG CACAGAGCTC CATTGCTGAG TGTGCTTGCT 
GCATATATGC AGGACACCAA CCAAATTGCT TGCCTGTGTG TGTGGCTTGT AACCTCTGTA 
GATCCGGCCA ATTGTGCAGA ACTCACAAAT ATGCTTTCGG TTGCTTCAGA TCATGAGTGG 
GACTTGCAAG ATCTGACACG GATTTGGGAA ATCTTGCTGG AGAGAAAAGA TAGCCAGACT 
TTGTACAAAG CATTCAGCAT CTTTTTAGAG GACTGTCCCC TTCTTCTCTT ACTTGAACTG 
TATGAGCTTT GCCTGCAACA TAAATACTAT CTGGAGGCTG GACAGAAAAT AGATGATTTC 
CAAAGATGGA TGATGAACGA TGACACTGAA GATACAGAGT TATCGCTTCT CATTCCACTG 
CCCTGGTTGC TACAAAGAGC TTCTCAACTT CTGCAGCTCC TTCTCTTGCA GACCCAGACT 
CCATATGAAT TGCGGAAGGT TTTGCAGCTT CTGAGTGACA AAGCTAAACC ACAGTTATTT 
GGCGGAATAG ATGTTCATAA GCTGAGTGAT CTAACCTCTA TTCTTCAAGA CCACCCTATT 
GCCATTAGCA AAGAATTAAT ATGCCACTAT AGTCCTGAAG CCCTGCAGGT TGAGTGTCAA 
AGGCTTATAC AGATGCTGTT GGACAAAAGG ATTTTTTCTC TTGCACAAAG AGTAGCAGAA 
CTAGCAGAAT TGCCCACTGA CAGTATTGTC ATAGAAGAGG TGTTGCAGGA CCAATGTCTC 
TTAAAAGAGG TTGGGCAGTG GGAGAATTCT CAAAGCCGTG TACAGTACTG GAGAAAATGC 
CACCAGGTGT TTAGCGCCAC GGAGCTCTCC CCAGCTGCAG CGTCTGACTT CTTCTGCTCT 
CAGACTCTTT CTCTTGTAGC GCCTGAATGC TTGCTTAGGG ATCAGACAGA GTTTTTGGCA 
GAGCAGGAGA TGCTGTTTAC CCTTGCTGGG CACTGGAGGT CCCTAAGTAA CACTGCTTCT 
ATAGAAGTCC TGGAGGAAAT TGAGCAGAAG ATTTGGAAAT GTCGCCTGGC AAGAGAAGTA 
TTGAGTAAAC ATGGAGCTGC TAGTCAACTC TGTACTCTGC CTTCATTTGC TAGCTTGACC 
CTGGAGTTTT CATTCTCATC TTTGCCTGCA CTAAACTCTC CTTCGCTTCT TCATATCTCT 
AATTTGCCAC CGCTTGGTAC TCCTAGCAGT TTGTTGGACA GAGAACAGAC CAAAGCATTG 
AGCTCTTTGG TTGACTGCCT GTTGGATGAG AGCCGTGTGC ACGAGGCCAG TCGGGTATGC 
AGGTACTTTC TGCTACCTCA TTATGATCTT TGGCTACTGC TGAACTGCAG GGCACTAGCA 
ACAGGAGAGA CCGTAAGGGA CCAACTGCAT CCGGAAATTC AAGCTATCCT CGGTGAAGGC 
AGCGAACGCC GGGAAAGCAT ATGGCACCAG AGGAAAAGAC TGCACAGCTC CTCCTCTCTG 
GACACCGCCA TGCCCCCTGT ACCCAGTGAT CCTGTGCTCT TAGATCTGGA AATTCTGAAG 
GACAGGTGCT CCCATGGCAA AACCTACTGC CAACAGCTGC TGTGTCTTTA CGAGTTAAGC 
CAGGACCTGG GATGTTCCTT CTCAGATATC TCCTCTCGAG ATCCCGGCGA GATTCTACGC 
TCTCTGCTTT CTTCTCAGCG TCCCGAGCTC ACAGACAGAG CACAAGCAGT CATCAACTTC 
CACGGACTGT CGCCGCAAAC AGTGGCACAG ATTGTGGCAG AAGAGGGTGT GAGAGTCTGG 
CGGGCAGGGC CCGTGGAAGT TTATAATGCC TCTGAAATAA GGCAGCGCTT TTTGCAGCTT 
GTAAAACTTT GCCAGGACCC AACTCTGGTT GGATTAACGC TACTTGACTA TCTAGAAAAT 
GTGCCACTGA CTGAGCAACA CTGCATTATT GAACTCCTGA TTTCTGCCCA CGATTGCTTC 
AGTTTAACGT GCCATCTTGA AGGAATTCGA CGGGTGCTTC AGGCATGTCG ACACCTAACC 
GAGAGTCACT TGGCGGCTAA CCAAGAATAC AGCCTTATGG TTCGCCTACT CTCTGGGATT 
GGTCGTTATA ATGAGATGGT TTATGTGTTT GACATTCTGC ACAAGGAACA GCACTTTGAG 
GTTCTGCTGA GGAAACAGCT AGATACAAAG GGGGGACTGC AGACTGCTCT TTTGGAATAC 
ATTAAGCGCT GCCATCCAGG AGACAGCGAG AAGCACAACA TGACTGCGCT CTGCTTCAGT 
TTGCACCGTG ACATTGGCCA CAACCATGAG CAGGCAGCTT TGATCCAGCT CAAACTCATC 
CAGTCTAGGG CATGGGAATA CTGGATGTCT GAATTGGTGG AGTTGAGATC TGCACTAATG 
AAAGCACTTA CCCTCCTAAT AGATGCAGCT GAGAGCTACT CAAAGGATTC CTGCGTCCGT 
CAGTCTCTGC GCTGTGCTCG CCTTACCCGT CTGTTGACTC TGCAGCTTCA TCTGCTAAAC 
AACAGTCATC AAACTAAATT AATCAACCTG GATAGAGAGA ACTTGATGGA GCCAATACTG 
GAACTGCCAC GTTTCTACCA GGCAGTCATT GTTACAGAGG CCTATGATAT ACAGCCAGAC 
TGGGCAGAGG TTTTATATCA CAAGGTGATT CTTGCTGGAG ACTTTCAGTA TTTAAATGAA 
TTCAAGCAGC GAGAACTGCT ACGGAGTGGA GTGTTCCAGC AACTGTCTGA CAAGTGTAAA 
TTACAGCCTC CAGGAGCTAC CGGACTTCAA AATCTGAAGA GAGTGCTCGG TTACTGCGAA 
GATACCTATA CCCGCTACAA ACTGGCCTTG GAAAACCAGT TCTATGATGT GACCGATGTT 
TTAATGAGAG ACTCACAAAC CCGCTGCTGC CTGACTGACA TGCTGTCTCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( XM_012958758.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814212.2 Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), transcript variant X1, mRNA.

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CloneID	OXa53409
Clone ID Related Accession (Same CDS sequence)	XM_012958758.2
Accession Version	XM_012958758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGGCTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGATATGG GTTTCTCACA CCCAAGAAAT 
CTGTGGTGTC ATCTTACTTT ATGGCATGCA TGGCAGAGGA TTTCTTCATG GATAGAATCT 
TTAGAAAAAC ATGGAAAGAC AAGTAACAAT GATCTCTCAA CATGGCCTCT TCTGACTCCA 
GAAATAGTGG AGTCATGCAC CCTCTGTTGT GACTATACAA AGCAGAAGAT CCTGGACAAG 
TTAGCAAGAA TGGAAATATT TGTCCCTTCT GAGCAGCAGA ATTTTGAGGC CCTTGTGGAA 
CGTATGGCAG TTAGTAGGAG CATGATGCCG TTTCCTGTCT CCAACCCTCA GTGCAGCACC 
TCTGAGAATC AAGATCGTCA CACCCACTTC GTTTTGCTGT GTGCTGAGCA GAGTCTTCAC 
TACCTATTGT ACACATATCT GGATTATCAC AGGTTGAATT CTCAGCTTTG CTCTGCTTTG 
GGCAATACAA CCTTGCATGA AACCTTGCCT TGGTTTGCAT TTTTGGTACA GATACGAGGT 
GTCAAAGACA ACTCTGAAGA TCCAAGCCGC ATGTTTAATG CTAGCCTCTC CAATGCCCAT 
ATGGTGATGC CAGGGTACCA GCCTAGTATG AGCAGCATGC TGCTGGAGGG ACACACATTG 
CTCGCCCTTG CAACCAATAT GTATGCACCT GGTGGCATTG ACAGGGTGTT GGAGCAGCAT 
GGAGAGTCAG ATTCTGGTCA TAGAAATGTA GACCCTCAGC TTCTTAAAAT GGCTTTAGCT 
CCATATCCGA AGCTCAAGGC AGCTCTCTTC AGTACACACC CAGCTACTGT CAGTTCTCCC 
TCTGACATTT CACTTTACCA TCTCCTACAG GCTCTCTCCC CATTTCAGCC TACACACTTC 
TTCACATGGC AGTCGGTAAA TACCCTTGCT GCAAGTGACA ACAGTGCAGT GCTCCCACAT 
TGCTCAAGTC CAGCCTTGGT CAGCAAGTTC TCTGTGGGTG AGCAACTAGA TGTCTTCTTC 
TTCCTGCGGA GCGGGCGACC ATCAGTCGCA TTCGCTACCT TTCTGGTGTT TCAGCTACTA 
AGAAGCAAAA CCCCCCAACA GCTAATCAAG CAAGTTGCTG AGGATGTTTA TTCCCTGGCA 
CTGTCTTCAT TTCATCTCTC CTCTGTGCTT GCTTCATGCT GCTGCTTCTT TGAGTTACTG 
GGTCTCAACA GCCACAAGCT TCGAGTGGAT GTGAACGTTG CACACATGAT ACTAAGTCAC 
TCAGCTTCTC TGCATGAGGA TCCAGGGGTA CACAAATCCC AGGCACAAGC TTTAGCTCAC 
AGACTTTGCA GACTGGTGGA GAATGAGAGA GAAGCAGCCA TGGATATTTT ACATAGCCTG 
GAGGAAGCTG TATTTTCTGA AGAGTGTCAA AACAGCTCAT CTCTACCCAC TGGCTCCTGT 
TTAACTATTC AGCAGTTCTG CCTCCTCCAT TCTATTCCCA TGAGCACTAG GTATTTGAGG 
AGCTGCGCAC AGCAACAAGA CTGGCTGCAG TTACTTGTGA ACACACACAC TCAGGAACAG 
GTCCTATGTG TAGCTGAAAA GTTGAGCCCG GCACTTAATA ATCACATAAT GCTATCATTG 
CAAGATGCAA ATTCAGATGC ACAAATCTCT GAATATGACT TGGAAAGATC TGAAAATGAT 
ACAGTGACTG CCAGTTTGTA CAATATATTG CTTGGGTGCC AGAAGACTGG GCAGCCAGGC 
CGCACACTTC TAAAGGAATG TATTAAGCAC AGAGCTCCAT TGCTGAGTGT GCTTGCTGCA 
TATATGCAGG ACACCAACCA AATTGCTTGC CTGTGTGTGT GGCTTGTAAC CTCTGTAGAT 
CCGGCCAATT GTGCAGAACT CACAAATATG CTTTCGGTTG CTTCAGATCA TGAGTGGGAC 
TTGCAAGATC TGACACGGAT TTGGGAAATC TTGCTGGAGA GAAAAGATAG CCAGACTTTG 
TACAAAGCAT TCAGCATCTT TTTAGAGGAC TGTCCCCTTC TTCTCTTACT TGAACTGTAT 
GAGCTTTGCC TGCAACATAA ATACTATCTG GAGGCTGGAC AGAAAATAGA TGATTTCCAA 
AGATGGATGA TGAACGATGA CACTGAAGAT ACAGAGTTAT CGCTTCTCAT TCCACTGCCC 
TGGTTGCTAC AAAGAGCTTC TCAACTTCTG CAGCTCCTTC TCTTGCAGAC CCAGACTCCA 
TATGAATTGC GGAAGGTTTT GCAGCTTCTG AGTGACAAAG CTAAACCACA GTTATTTGGC 
GGAATAGATG TTCATAAGCT GAGTGATCTA ACCTCTATTC TTCAAGACCA CCCTATTGCC 
ATTAGCAAAG AATTAATATG CCACTATAGT CCTGAAGCCC TGCAGGTTGA GTGTCAAAGG 
CTTATACAGA TGCTGTTGGA CAAAAGGATT TTTTCTCTTG CACAAAGAGT AGCAGAACTA 
GCAGAATTGC CCACTGACAG TATTGTCATA GAAGAGGTGT TGCAGGACCA ATGTCTCTTA 
AAAGAGGTTG GGCAGTGGGA GAATTCTCAA AGCCGTGTAC AGTACTGGAG AAAATGCCAC 
CAGGTGTTTA GCGCCACGGA GCTCTCCCCA GCTGCAGCGT CTGACTTCTT CTGCTCTCAG 
ACTCTTTCTC TTGTAGCGCC TGAATGCTTG CTTAGGGATC AGACAGAGTT TTTGGCAGAG 
CAGGAGATGC TGTTTACCCT TGCTGGGCAC TGGAGGTCCC TAAGTAACAC TGCTTCTATA 
GAAGTCCTGG AGGAAATTGA GCAGAAGATT TGGAAATGTC GCCTGGCAAG AGAAGTATTG 
AGTAAACATG GAGCTGCTAG TCAACTCTGT ACTCTGCCTT CATTTGCTAG CTTGACCCTG 
GAGTTTTCAT TCTCATCTTT GCCTGCACTA AACTCTCCTT CGCTTCTTCA TATCTCTAAT 
TTGCCACCGC TTGGTACTCC TAGCAGTTTG TTGGACAGAG AACAGACCAA AGCATTGAGC 
TCTTTGGTTG ACTGCCTGTT GGATGAGAGC CGTGTGCACG AGGCCAGTCG GGTATGCAGG 
TACTTTCTGC TACCTCATTA TGATCTTTGG CTACTGCTGA ACTGCAGGGC ACTAGCAACA 
GGAGAGACCG TAAGGGACCA ACTGCATCCG GAAATTCAAG CTATCCTCGG TGAAGGCAGC 
GAACGCCGGG AAAGCATATG GCACCAGAGG AAAAGACTGC ACAGCTCCTC CTCTCTGGAC 
ACCGCCATGC CCCCTGTACC CAGTGATCCT GTGCTCTTAG ATCTGGAAAT TCTGAAGGAC 
AGGTGCTCCC ATGGCAAAAC CTACTGCCAA CAGCTGCTGT GTCTTTACGA GTTAAGCCAG 
GACCTGGGAT GTTCCTTCTC AGATATCTCC TCTCGAGATC CCGGCGAGAT TCTACGCTCT 
CTGCTTTCTT CTCAGCGTCC CGAGCTCACA GACAGAGCAC AAGCAGTCAT CAACTTCCAC 
GGACTGTCGC CGCAAACAGT GGCACAGATT GTGGCAGAAG AGGGTGTGAG AGTCTGGCGG 
GCAGGGCCCG TGGAAGTTTA TAATGCCTCT GAAATAAGGC AGCGCTTTTT GCAGCTTGTA 
AAACTTTGCC AGGACCCAAC TCTGGTTGGA TTAACGCTAC TTGACTATCT AGAAAATGTG 
CCACTGACTG AGCAACACTG CATTATTGAA CTCCTGATTT CTGCCCACGA TTGCTTCAGT 
TTAACGTGCC ATCTTGAAGG AATTCGACGG GTGCTTCAGG CATGTCGACA CCTAACCGAG 
AGTCACTTGG CGGCTAACCA AGAATACAGC CTTATGGTTC GCCTACTCTC TGGGATTGGT 
CGTTATAATG AGATGGTTTA TGTGTTTGAC ATTCTGCACA AGGAACAGCA CTTTGAGGTT 
CTGCTGAGGA AACAGCTAGA TACAAAGGGG GGACTGCAGA CTGCTCTTTT GGAATACATT 
AAGCGCTGCC ATCCAGGAGA CAGCGAGAAG CACAACATGA CTGCGCTCTG CTTCAGTTTG 
CACCGTGACA TTGGCCACAA CCATGAGCAG GCAGCTTTGA TCCAGCTCAA ACTCATCCAG 
TCTAGGGCAT GGGAATACTG GATGTCTGAA TTGGTGGAGT TGAGATCTGC ACTAATGAAA 
GCACTTACCC TCCTAATAGA TGCAGCTGAG AGCTACTCAA AGGATTCCTG CGTCCGTCAG 
TCTCTGCGCT GTGCTCGCCT TACCCGTCTG TTGACTCTGC AGCTTCATCT GCTAAACAAC 
AGTCATCAAA CTAAATTAAT CAACCTGGAT AGAGAGAACT TGATGGAGCC AATACTGGAA 
CTGCCACGTT TCTACCAGGC AGTCATTGTT ACAGAGGCCT ATGATATACA GCCAGACTGG 
GCAGAGGTTT TATATCACAA GGTGATTCTT GCTGGAGACT TTCAGTATTT AAATGAATTC 
AAGCAGCGAG AACTGCTACG GAGTGGAGTG TTCCAGCAAC TGTCTGACAA GTGTAAATTA 
CAGCCTCCAG GAGCTACCGG ACTTCAAAAT CTGAAGAGAG TGCTCGGTTA CTGCGAAGAT 
ACCTATACCC GCTACAAACT GGCCTTGGAA AACCAGTTCT ATGATGTGAC CGATGTTTTA 
ATGAGAGACT CACAAACCCG CTGCTGCCTG ACTGACATGC TGTCTCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814212.2
CDS	215..7285
Translation	MGRRILQSCKMNLNILLVPGLTLPEGTNPPVRVSLTRQKHPTLCSLCPQGELHLTALPYEGFHTTHTQLEGLFSHFLWENADGNEEPSDNIPRLLTLSGSNEVAVHGLHVADGHLNVTTIFLCDEDTLRKLCADNHVCVSSVICVRLLSLQGDDVLLLLSSSVLLHLHFSSEDNSLKLLSCLCLDLGFEATERISDVCLKSGLLFMLDTYGFVYVYDTVDGSPIFTMELPLRPENAALDPLNSICVSDDLGLFVVTTAASWALSVQLCEDAREISDHAVSAWHSDHVPDLHISGADEERLSSCHESVCTPRCTLRSDRSWDATLSSLCNEIMNSSDTFVCSKNPYLWPCNLPLKVPLSGIPVGEGWVRIVSEGLDDPLSLHIGSVTSFSAVLCLFGMDGLLNMIQWDMQSKEISSYPLGKAHFVHCSQEDACLVMSDRGLSLVVFSASQEEFLTRLMIHGSASTADTLCLLNKWGRCSVPIHTLEAGLDNRQLDTVDFFLKSKVSILNPLISEEPPDGIQAERYLQNVQDLLPALDLLVSYIRETDQETQSKHFSEQLLQLTLGFLNGQLHLLCALLSESSPALQQAVEILMGYITKLRRFMKKFMEQPQPVDVPGIEGQLHSWKNLSLEQIISDAILTNRIPEAQVFLRAEGHQACSLTWLKQEGLNLVYKCLLETRLQEARQLLRNMGFSVMNELKRICLHTSDRGVRTLLVDLLQREGCFSDQEQEDIQTVIHLEEVYCCPQKRTKESHKLLPHRLVQRLFTGSQEPEVLQAALTPSKNKSHMVLLYWAQGWDLETKEAILLPLRNSQDMGFSHPRNLWCHLTLWHAWQRISSWIESLEKHGKTSNNDLSTWPLLTPEIVESCTLCCDYTKQKILDKLARMEIFVPSEQQNFEALVERMAVSRSMMPFPVSNPQCSTSENQDRHTHFVLLCAEQSLHYLLYTYLDYHRLNSQLCSALGNTTLHETLPWFAFLVQIRGVKDNSEDPSRMFNASLSNAHMVMPGYQPSMSSMLLEGHTLLALATNMYAPGGIDRVLEQHGESDSGHRNVDPQLLKMALAPYPKLKAALFSTHPATVSSPSDISLYHLLQALSPFQPTHFFTWQSVNTLAASDNSAVLPHCSSPALVSKFSVGEQLDVFFFLRSGRPSVAFATFLVFQLLRSKTPQQLIKQVAEDVYSLALSSFHLSSVLASCCCFFELLGLNSHKLRVDVNVAHMILSHSASLHEDPGVHKSQAQALAHRLCRLVENEREAAMDILHSLEEAVFSEECQNSSSLPTGSCLTIQQFCLLHSIPMSTRYLRSCAQQQDWLQLLVNTHTQEQVLCVAEKLSPALNNHIMLSLQDANSDAQISEYDLERSENDTVTASLYNILLGCQKTGQPGRTLLKECIKHRAPLLSVLAAYMQDTNQIACLCVWLVTSVDPANCAELTNMLSVASDHEWDLQDLTRIWEILLERKDSQTLYKAFSIFLEDCPLLLLLELYELCLQHKYYLEAGQKIDDFQRWMMNDDTEDTELSLLIPLPWLLQRASQLLQLLLLQTQTPYELRKVLQLLSDKAKPQLFGGIDVHKLSDLTSILQDHPIAISKELICHYSPEALQVECQRLIQMLLDKRIFSLAQRVAELAELPTDSIVIEEVLQDQCLLKEVGQWENSQSRVQYWRKCHQVFSATELSPAAASDFFCSQTLSLVAPECLLRDQTEFLAEQEMLFTLAGHWRSLSNTASIEVLEEIEQKIWKCRLAREVLSKHGAASQLCTLPSFASLTLEFSFSSLPALNSPSLLHISNLPPLGTPSSLLDREQTKALSSLVDCLLDESRVHEASRVCRYFLLPHYDLWLLLNCRALATGETVRDQLHPEIQAILGEGSERRESIWHQRKRLHSSSSLDTAMPPVPSDPVLLDLEILKDRCSHGKTYCQQLLCLYELSQDLGCSFSDISSRDPGEILRSLLSSQRPELTDRAQAVINFHGLSPQTVAQIVAEEGVRVWRAGPVEVYNASEIRQRFLQLVKLCQDPTLVGLTLLDYLENVPLTEQHCIIELLISAHDCFSLTCHLEGIRRVLQACRHLTESHLAANQEYSLMVRLLSGIGRYNEMVYVFDILHKEQHFEVLLRKQLDTKGGLQTALLEYIKRCHPGDSEKHNMTALCFSLHRDIGHNHEQAALIQLKLIQSRAWEYWMSELVELRSALMKALTLLIDAAESYSKDSCVRQSLRCARLTRLLTLQLHLLNNSHQTKLINLDRENLMEPILELPRFYQAVIVTEAYDIQPDWAEVLYHKVILAGDFQYLNEFKQRELLRSGVFQQLSDKCKLQPPGATGLQNLKRVLGYCEDTYTRYKLALENQFYDVTDVLMRDSQTRCCLTDMLSR

Target ORF information:

RefSeq Version	XM_012958758.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958758.2

ORF Insert Sequence:

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGGCTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGATATGG GTTTCTCACA CCCAAGAAAT 
CTGTGGTGTC ATCTTACTTT ATGGCATGCA TGGCAGAGGA TTTCTTCATG GATAGAATCT 
TTAGAAAAAC ATGGAAAGAC AAGTAACAAT GATCTCTCAA CATGGCCTCT TCTGACTCCA 
GAAATAGTGG AGTCATGCAC CCTCTGTTGT GACTATACAA AGCAGAAGAT CCTGGACAAG 
TTAGCAAGAA TGGAAATATT TGTCCCTTCT GAGCAGCAGA ATTTTGAGGC CCTTGTGGAA 
CGTATGGCAG TTAGTAGGAG CATGATGCCG TTTCCTGTCT CCAACCCTCA GTGCAGCACC 
TCTGAGAATC AAGATCGTCA CACCCACTTC GTTTTGCTGT GTGCTGAGCA GAGTCTTCAC 
TACCTATTGT ACACATATCT GGATTATCAC AGGTTGAATT CTCAGCTTTG CTCTGCTTTG 
GGCAATACAA CCTTGCATGA AACCTTGCCT TGGTTTGCAT TTTTGGTACA GATACGAGGT 
GTCAAAGACA ACTCTGAAGA TCCAAGCCGC ATGTTTAATG CTAGCCTCTC CAATGCCCAT 
ATGGTGATGC CAGGGTACCA GCCTAGTATG AGCAGCATGC TGCTGGAGGG ACACACATTG 
CTCGCCCTTG CAACCAATAT GTATGCACCT GGTGGCATTG ACAGGGTGTT GGAGCAGCAT 
GGAGAGTCAG ATTCTGGTCA TAGAAATGTA GACCCTCAGC TTCTTAAAAT GGCTTTAGCT 
CCATATCCGA AGCTCAAGGC AGCTCTCTTC AGTACACACC CAGCTACTGT CAGTTCTCCC 
TCTGACATTT CACTTTACCA TCTCCTACAG GCTCTCTCCC CATTTCAGCC TACACACTTC 
TTCACATGGC AGTCGGTAAA TACCCTTGCT GCAAGTGACA ACAGTGCAGT GCTCCCACAT 
TGCTCAAGTC CAGCCTTGGT CAGCAAGTTC TCTGTGGGTG AGCAACTAGA TGTCTTCTTC 
TTCCTGCGGA GCGGGCGACC ATCAGTCGCA TTCGCTACCT TTCTGGTGTT TCAGCTACTA 
AGAAGCAAAA CCCCCCAACA GCTAATCAAG CAAGTTGCTG AGGATGTTTA TTCCCTGGCA 
CTGTCTTCAT TTCATCTCTC CTCTGTGCTT GCTTCATGCT GCTGCTTCTT TGAGTTACTG 
GGTCTCAACA GCCACAAGCT TCGAGTGGAT GTGAACGTTG CACACATGAT ACTAAGTCAC 
TCAGCTTCTC TGCATGAGGA TCCAGGGGTA CACAAATCCC AGGCACAAGC TTTAGCTCAC 
AGACTTTGCA GACTGGTGGA GAATGAGAGA GAAGCAGCCA TGGATATTTT ACATAGCCTG 
GAGGAAGCTG TATTTTCTGA AGAGTGTCAA AACAGCTCAT CTCTACCCAC TGGCTCCTGT 
TTAACTATTC AGCAGTTCTG CCTCCTCCAT TCTATTCCCA TGAGCACTAG GTATTTGAGG 
AGCTGCGCAC AGCAACAAGA CTGGCTGCAG TTACTTGTGA ACACACACAC TCAGGAACAG 
GTCCTATGTG TAGCTGAAAA GTTGAGCCCG GCACTTAATA ATCACATAAT GCTATCATTG 
CAAGATGCAA ATTCAGATGC ACAAATCTCT GAATATGACT TGGAAAGATC TGAAAATGAT 
ACAGTGACTG CCAGTTTGTA CAATATATTG CTTGGGTGCC AGAAGACTGG GCAGCCAGGC 
CGCACACTTC TAAAGGAATG TATTAAGCAC AGAGCTCCAT TGCTGAGTGT GCTTGCTGCA 
TATATGCAGG ACACCAACCA AATTGCTTGC CTGTGTGTGT GGCTTGTAAC CTCTGTAGAT 
CCGGCCAATT GTGCAGAACT CACAAATATG CTTTCGGTTG CTTCAGATCA TGAGTGGGAC 
TTGCAAGATC TGACACGGAT TTGGGAAATC TTGCTGGAGA GAAAAGATAG CCAGACTTTG 
TACAAAGCAT TCAGCATCTT TTTAGAGGAC TGTCCCCTTC TTCTCTTACT TGAACTGTAT 
GAGCTTTGCC TGCAACATAA ATACTATCTG GAGGCTGGAC AGAAAATAGA TGATTTCCAA 
AGATGGATGA TGAACGATGA CACTGAAGAT ACAGAGTTAT CGCTTCTCAT TCCACTGCCC 
TGGTTGCTAC AAAGAGCTTC TCAACTTCTG CAGCTCCTTC TCTTGCAGAC CCAGACTCCA 
TATGAATTGC GGAAGGTTTT GCAGCTTCTG AGTGACAAAG CTAAACCACA GTTATTTGGC 
GGAATAGATG TTCATAAGCT GAGTGATCTA ACCTCTATTC TTCAAGACCA CCCTATTGCC 
ATTAGCAAAG AATTAATATG CCACTATAGT CCTGAAGCCC TGCAGGTTGA GTGTCAAAGG 
CTTATACAGA TGCTGTTGGA CAAAAGGATT TTTTCTCTTG CACAAAGAGT AGCAGAACTA 
GCAGAATTGC CCACTGACAG TATTGTCATA GAAGAGGTGT TGCAGGACCA ATGTCTCTTA 
AAAGAGGTTG GGCAGTGGGA GAATTCTCAA AGCCGTGTAC AGTACTGGAG AAAATGCCAC 
CAGGTGTTTA GCGCCACGGA GCTCTCCCCA GCTGCAGCGT CTGACTTCTT CTGCTCTCAG 
ACTCTTTCTC TTGTAGCGCC TGAATGCTTG CTTAGGGATC AGACAGAGTT TTTGGCAGAG 
CAGGAGATGC TGTTTACCCT TGCTGGGCAC TGGAGGTCCC TAAGTAACAC TGCTTCTATA 
GAAGTCCTGG AGGAAATTGA GCAGAAGATT TGGAAATGTC GCCTGGCAAG AGAAGTATTG 
AGTAAACATG GAGCTGCTAG TCAACTCTGT ACTCTGCCTT CATTTGCTAG CTTGACCCTG 
GAGTTTTCAT TCTCATCTTT GCCTGCACTA AACTCTCCTT CGCTTCTTCA TATCTCTAAT 
TTGCCACCGC TTGGTACTCC TAGCAGTTTG TTGGACAGAG AACAGACCAA AGCATTGAGC 
TCTTTGGTTG ACTGCCTGTT GGATGAGAGC CGTGTGCACG AGGCCAGTCG GGTATGCAGG 
TACTTTCTGC TACCTCATTA TGATCTTTGG CTACTGCTGA ACTGCAGGGC ACTAGCAACA 
GGAGAGACCG TAAGGGACCA ACTGCATCCG GAAATTCAAG CTATCCTCGG TGAAGGCAGC 
GAACGCCGGG AAAGCATATG GCACCAGAGG AAAAGACTGC ACAGCTCCTC CTCTCTGGAC 
ACCGCCATGC CCCCTGTACC CAGTGATCCT GTGCTCTTAG ATCTGGAAAT TCTGAAGGAC 
AGGTGCTCCC ATGGCAAAAC CTACTGCCAA CAGCTGCTGT GTCTTTACGA GTTAAGCCAG 
GACCTGGGAT GTTCCTTCTC AGATATCTCC TCTCGAGATC CCGGCGAGAT TCTACGCTCT 
CTGCTTTCTT CTCAGCGTCC CGAGCTCACA GACAGAGCAC AAGCAGTCAT CAACTTCCAC 
GGACTGTCGC CGCAAACAGT GGCACAGATT GTGGCAGAAG AGGGTGTGAG AGTCTGGCGG 
GCAGGGCCCG TGGAAGTTTA TAATGCCTCT GAAATAAGGC AGCGCTTTTT GCAGCTTGTA 
AAACTTTGCC AGGACCCAAC TCTGGTTGGA TTAACGCTAC TTGACTATCT AGAAAATGTG 
CCACTGACTG AGCAACACTG CATTATTGAA CTCCTGATTT CTGCCCACGA TTGCTTCAGT 
TTAACGTGCC ATCTTGAAGG AATTCGACGG GTGCTTCAGG CATGTCGACA CCTAACCGAG 
AGTCACTTGG CGGCTAACCA AGAATACAGC CTTATGGTTC GCCTACTCTC TGGGATTGGT 
CGTTATAATG AGATGGTTTA TGTGTTTGAC ATTCTGCACA AGGAACAGCA CTTTGAGGTT 
CTGCTGAGGA AACAGCTAGA TACAAAGGGG GGACTGCAGA CTGCTCTTTT GGAATACATT 
AAGCGCTGCC ATCCAGGAGA CAGCGAGAAG CACAACATGA CTGCGCTCTG CTTCAGTTTG 
CACCGTGACA TTGGCCACAA CCATGAGCAG GCAGCTTTGA TCCAGCTCAA ACTCATCCAG 
TCTAGGGCAT GGGAATACTG GATGTCTGAA TTGGTGGAGT TGAGATCTGC ACTAATGAAA 
GCACTTACCC TCCTAATAGA TGCAGCTGAG AGCTACTCAA AGGATTCCTG CGTCCGTCAG 
TCTCTGCGCT GTGCTCGCCT TACCCGTCTG TTGACTCTGC AGCTTCATCT GCTAAACAAC 
AGTCATCAAA CTAAATTAAT CAACCTGGAT AGAGAGAACT TGATGGAGCC AATACTGGAA 
CTGCCACGTT TCTACCAGGC AGTCATTGTT ACAGAGGCCT ATGATATACA GCCAGACTGG 
GCAGAGGTTT TATATCACAA GGTGATTCTT GCTGGAGACT TTCAGTATTT AAATGAATTC 
AAGCAGCGAG AACTGCTACG GAGTGGAGTG TTCCAGCAAC TGTCTGACAA GTGTAAATTA 
CAGCCTCCAG GAGCTACCGG ACTTCAAAAT CTGAAGAGAG TGCTCGGTTA CTGCGAAGAT 
ACCTATACCC GCTACAAACT GGCCTTGGAA AACCAGTTCT ATGATGTGAC CGATGTTTTA 
ATGAGAGACT CACAAACCCG CTGCTGCCTG ACTGACATGC TGTCTCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( XM_031897783.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753643.1 Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), transcript variant X2, mRNA.

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CloneID	OXa53410
Clone ID Related Accession (Same CDS sequence)	XM_031897783.1
Accession Version	XM_031897783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTTA GTGTGTCCTC AGTCATCTGT GTTCGGCTAT TGTCCCTACA AGGAGATGAT 
GTTCTGCTGT TGCTGAGTAG CAGTGTCTTG TTGCATCTTC ACTTTTCTTC AGAAGATAAC 
AGTCTAAAAC TTTTATCCTG CTTATGCTTG GATTTGGGTT TCGAAGCAAC AGAGCGCATT 
TCAGATGTAT GTTTAAAAAG TGGGCTGTTG TTTATGCTGG ATACTTATGG TTTTGTATAT 
GTTTATGATA CTGTAGATGG CTCACCCATC TTCACCATGG AACTTCCACT AAGACCAGAG 
AATGCTGCTT TAGATCCACT CAACAGTATT TGTGTGTCTG ATGACCTAGG CTTATTTGTA 
GTCACCACTG CTGCTTCCTG GGCTCTCTCT GTGCAACTCT GTGAAGATGC CAGAGAGATC 
TCAGATCACG CAGTATCTGC CTGGCACAGT GATCATGTCC CAGATCTACA TATATCAGGT 
GCAGATGAAG AGAGACTGAG TAGTTGTCAT GAAAGTGTTT GTACCCCTAG ATGTACCTTA 
CGCTCAGACA GATCTTGGGA TGCCACACTG TCTTCACTAT GCAATGAGAT CATGAACTCT 
TCAGACACGT TTGTGTGCTC CAAGAACCCT TATCTATGGC CTTGTAACCT TCCTTTAAAA 
GTACCTCTCA GTGGGATCCC TGTGGGGGAA GGCTGGGTGA GGATTGTTTC TGAAGGGTTG 
GATGATCCTT TGAGTCTCCA CATCGGGTCT GTCACTTCCT TCAGTGCTGT ACTGTGTCTT 
TTTGGGATGG ATGGCTTACT TAACATGATA CAATGGGACA TGCAATCAAA GGAAATCTCT 
TCCTATCCCT TGGGAAAGGC TCACTTTGTT CACTGTTCTC AGGAAGATGC TTGCCTGGTG 
ATGTCAGATC GAGGGCTATC TCTAGTGGTG TTTTCTGCTT CACAAGAGGA ATTCCTGACC 
AGATTAATGA TTCATGGCAG TGCAAGTACA GCAGATACCT TATGTCTTCT AAATAAGTGG 
GGCCGTTGCT CCGTTCCCAT TCACACACTA GAGGCTGGTC TAGACAACAG ACAGTTAGAC 
ACTGTTGACT TTTTCCTGAA GAGCAAAGTA AGCATCCTAA ACCCTTTGAT CTCTGAGGAG 
CCTCCCGATG GCATCCAGGC GGAACGCTAT CTGCAAAATG TGCAGGATCT TCTGCCAGCT 
CTTGATCTTC TTGTCTCCTA CATCAGAGAA ACAGACCAGG AGACACAAAG CAAGCACTTC 
TCTGAGCAGT TGTTGCAGCT CACTCTTGGC TTCCTGAATG GACAACTTCA TCTGCTCTGT 
GCGCTGCTAT CTGAGTCAAG TCCTGCATTG CAGCAAGCTG TTGAGATTCT TATGGGTTAT 
ATAACCAAGC TACGCCGCTT CATGAAGAAA TTTATGGAAC AGCCACAACC TGTGGACGTC 
CCAGGTATTG AGGGACAGTT ACACTCTTGG AAGAATCTGT CATTGGAGCA AATAATCTCT 
GATGCCATTT TGACAAACCG CATTCCTGAG GCCCAGGTAT TCCTGCGTGC TGAGGGTCAT 
CAAGCTTGCA GCCTGACATG GTTGAAACAA GAAGGCCTTA ATCTGGTGTA CAAATGCCTG 
CTGGAGACAC GCCTGCAAGA GGCTCGTCAG CTTCTCCGAA ATATGGGCTT TTCTGTCATG 
AATGAGCTTA AGCGCATTTG TTTGCACACA TCAGATCGGG GCGTCAGGAC ATTGCTGGTA 
GATCTCCTGC AGAGAGAGGG GTGTTTTTCA GACCAAGAGC AGGAAGATAT TCAAACTGTG 
ATCCATTTGG AAGAAGTTTA CTGTTGTCCA CAAAAAAGGA CTAAAGAGAG TCATAAACTT 
CTGCCTCACA GGCTTGTACA GAGGCTTTTC ACTGGATCTC AGGAACCAGA AGTCCTACAA 
GCAGCTTTGA CACCTTCTAA GAACAAGTCA CACATGGTCC TCCTCTACTG GGCTCAGGGC 
TGGGATTTGG AAACAAAGGA AGCTATTTTA CTGCCCCTGA GGAACAGTCA AGTAGATATG 
GGTTTCTCAC ACCCAAGAAA TCTGTGGTGT CATCTTACTT TATGGCATGC ATGGCAGAGG 
ATTTCTTCAT GGATAGAATC TTTAGAAAAA CATGGAAAGA CAAGTAACAA TGATCTCTCA 
ACATGGCCTC TTCTGACTCC AGAAATAGTG GAGTCATGCA CCCTCTGTTG TGACTATACA 
AAGCAGAAGA TCCTGGACAA GTTAGCAAGA ATGGAAATAT TTGTCCCTTC TGAGCAGCAG 
AATTTTGAGG CCCTTGTGGA ACGTATGGCA GTTAGTAGGA GCATGATGCC GTTTCCTGTC 
TCCAACCCTC AGTGCAGCAC CTCTGAGAAT CAAGATCGTC ACACCCACTT CGTTTTGCTG 
TGTGCTGAGC AGAGTCTTCA CTACCTATTG TACACATATC TGGATTATCA CAGGTTGAAT 
TCTCAGCTTT GCTCTGCTTT GGGCAATACA ACCTTGCATG AAACCTTGCC TTGGTTTGCA 
TTTTTGGTAC AGATACGAGG TGTCAAAGAC AACTCTGAAG ATCCAAGCCG CATGTTTAAT 
GCTAGCCTCT CCAATGCCCA TATGGTGATG CCAGGGTACC AGCCTAGTAT GAGCAGCATG 
CTGCTGGAGG GACACACATT GCTCGCCCTT GCAACCAATA TGTATGCACC TGGTGGCATT 
GACAGGGTGT TGGAGCAGCA TGGAGAGTCA GATTCTGGTC ATAGAAATGT AGACCCTCAG 
CTTCTTAAAA TGGCTTTAGC TCCATATCCG AAGCTCAAGG CAGCTCTCTT CAGTACACAC 
CCAGCTACTG TCAGTTCTCC CTCTGACATT TCACTTTACC ATCTCCTACA GGCTCTCTCC 
CCATTTCAGC CTACACACTT CTTCACATGG CAGTCGGTAA ATACCCTTGC TGCAAGTGAC 
AACAGTGCAG TGCTCCCACA TTGCTCAAGT CCAGCCTTGG TCAGCAAGTT CTCTGTGGGT 
GAGCAACTAG ATGTCTTCTT CTTCCTGCGG AGCGGGCGAC CATCAGTCGC ATTCGCTACC 
TTTCTGGTGT TTCAGCTACT AAGAAGCAAA ACCCCCCAAC AGCTAATCAA GCAAGTTGCT 
GAGGATGTTT ATTCCCTGGC ACTGTCTTCA TTTCATCTCT CCTCTGTGCT TGCTTCATGC 
TGCTGCTTCT TTGAGTTACT GGGTCTCAAC AGCCACAAGC TTCGAGTGGA TGTGAACGTT 
GCACACATGA TACTAAGTCA CTCAGCTTCT CTGCATGAGG ATCCAGGGGT ACACAAATCC 
CAGGCACAAG CTTTAGCTCA CAGACTTTGC AGACTGGTGG AGAATGAGAG AGAAGCAGCC 
ATGGATATTT TACATAGCCT GGAGGAAGCT GTATTTTCTG AAGAGTGTCA AAACAGCTCA 
TCTCTACCCA CTGGCTCCTG TTTAACTATT CAGCAGTTCT GCCTCCTCCA TTCTATTCCC 
ATGAGCACTA GGTATTTGAG GAGCTGCGCA CAGCAACAAG ACTGGCTGCA GTTACTTGTG 
AACACACACA CTCAGGAACA GGTCCTATGT GTAGCTGAAA AGTTGAGCCC GGCACTTAAT 
AATCACATAA TGCTATCATT GCAAGATGCA AATTCAGATG CACAAATCTC TGAATATGAC 
TTGGAAAGAT CTGAAAATGA TACAGTGACT GCCAGTTTGT ACAATATATT GCTTGGGTGC 
CAGAAGACTG GGCAGCCAGG CCGCACACTT CTAAAGGAAT GTATTAAGCA CAGAGCTCCA 
TTGCTGAGTG TGCTTGCTGC ATATATGCAG GACACCAACC AAATTGCTTG CCTGTGTGTG 
TGGCTTGTAA CCTCTGTAGA TCCGGCCAAT TGTGCAGAAC TCACAAATAT GCTTTCGGTT 
GCTTCAGATC ATGAGTGGGA CTTGCAAGAT CTGACACGGA TTTGGGAAAT CTTGCTGGAG 
AGAAAAGATA GCCAGACTTT GTACAAAGCA TTCAGCATCT TTTTAGAGGA CTGTCCCCTT 
CTTCTCTTAC TTGAACTGTA TGAGCTTTGC CTGCAACATA AATACTATCT GGAGGCTGGA 
CAGAAAATAG ATGATTTCCA AAGATGGATG ATGAACGATG ACACTGAAGA TACAGAGTTA 
TCGCTTCTCA TTCCACTGCC CTGGTTGCTA CAAAGAGCTT CTCAACTTCT GCAGCTCCTT 
CTCTTGCAGA CCCAGACTCC ATATGAATTG CGGAAGGTTT TGCAGCTTCT GAGTGACAAA 
GCTAAACCAC AGTTATTTGG CGGAATAGAT GTTCATAAGC TGAGTGATCT AACCTCTATT 
CTTCAAGACC ACCCTATTGC CATTAGCAAA GAATTAATAT GCCACTATAG TCCTGAAGCC 
CTGCAGGTTG AGTGTCAAAG GCTTATACAG ATGCTGTTGG ACAAAAGGAT TTTTTCTCTT 
GCACAAAGAG TAGCAGAACT AGCAGAATTG CCCACTGACA GTATTGTCAT AGAAGAGGTG 
TTGCAGGACC AATGTCTCTT AAAAGAGGTT GGGCAGTGGG AGAATTCTCA AAGCCGTGTA 
CAGTACTGGA GAAAATGCCA CCAGGTGTTT AGCGCCACGG AGCTCTCCCC AGCTGCAGCG 
TCTGACTTCT TCTGCTCTCA GACTCTTTCT CTTGTAGCGC CTGAATGCTT GCTTAGGGAT 
CAGACAGAGT TTTTGGCAGA GCAGGAGATG CTGTTTACCC TTGCTGGGCA CTGGAGGTCC 
CTAAGTAACA CTGCTTCTAT AGAAGTCCTG GAGGAAATTG AGCAGAAGAT TTGGAAATGT 
CGCCTGGCAA GAGAAGTATT GAGTAAACAT GGAGCTGCTA GTCAACTCTG TACTCTGCCT 
TCATTTGCTA GCTTGACCCT GGAGTTTTCA TTCTCATCTT TGCCTGCACT AAACTCTCCT 
TCGCTTCTTC ATATCTCTAA TTTGCCACCG CTTGGTACTC CTAGCAGTTT GTTGGACAGA 
GAACAGACCA AAGCATTGAG CTCTTTGGTT GACTGCCTGT TGGATGAGAG CCGTGTGCAC 
GAGGCCAGTC GGGTATGCAG GTACTTTCTG CTACCTCATT ATGATCTTTG GCTACTGCTG 
AACTGCAGGG CACTAGCAAC AGGAGAGACC GTAAGGGACC AACTGCATCC GGAAATTCAA 
GCTATCCTCG GTGAAGGCAG CGAACGCCGG GAAAGCATAT GGCACCAGAG GAAAAGACTG 
CACAGCTCCT CCTCTCTGGA CACCGCCATG CCCCCTGTAC CCAGTGATCC TGTGCTCTTA 
GATCTGGAAA TTCTGAAGGA CAGGTGCTCC CATGGCAAAA CCTACTGCCA ACAGCTGCTG 
TGTCTTTACG AGTTAAGCCA GGACCTGGGA TGTTCCTTCT CAGATATCTC CTCTCGAGAT 
CCCGGCGAGA TTCTACGCTC TCTGCTTTCT TCTCAGCGTC CCGAGCTCAC AGACAGAGCA 
CAAGCAGTCA TCAACTTCCA CGGACTGTCG CCGCAAACAG TGGCACAGAT TGTGGCAGAA 
GAGGGTGTGA GAGTCTGGCG GGCAGGGCCC GTGGAAGTTT ATAATGCCTC TGAAATAAGG 
CAGCGCTTTT TGCAGCTTGT AAAACTTTGC CAGGACCCAA CTCTGGTTGG ATTAACGCTA 
CTTGACTATC TAGAAAATGT GCCACTGACT GAGCAACACT GCATTATTGA ACTCCTGATT 
TCTGCCCACG ATTGCTTCAG TTTAACGTGC CATCTTGAAG GAATTCGACG GGTGCTTCAG 
GCATGTCGAC ACCTAACCGA GAGTCACTTG GCGGCTAACC AAGAATACAG CCTTATGGTT 
CGCCTACTCT CTGGGATTGG TCGTTATAAT GAGATGGTTT ATGTGTTTGA CATTCTGCAC 
AAGGAACAGC ACTTTGAGGT TCTGCTGAGG AAACAGCTAG ATACAAAGGG GGGACTGCAG 
ACTGCTCTTT TGGAATACAT TAAGCGCTGC CATCCAGGAG ACAGCGAGAA GCACAACATG 
ACTGCGCTCT GCTTCAGTTT GCACCGTGAC ATTGGCCACA ACCATGAGCA GGCAGCTTTG 
ATCCAGCTCA AACTCATCCA GTCTAGGGCA TGGGAATACT GGATGTCTGA ATTGGTGGAG 
TTGAGATCTG CACTAATGAA AGCACTTACC CTCCTAATAG ATGCAGCTGA GAGCTACTCA 
AAGGATTCCT GCGTCCGTCA GTCTCTGCGC TGTGCTCGCC TTACCCGTCT GTTGACTCTG 
CAGCTTCATC TGCTAAACAA CAGTCATCAA ACTAAATTAA TCAACCTGGA TAGAGAGAAC 
TTGATGGAGC CAATACTGGA ACTGCCACGT TTCTACCAGG CAGTCATTGT TACAGAGGCC 
TATGATATAC AGCCAGACTG GGCAGAGGTT TTATATCACA AGGTGATTCT TGCTGGAGAC 
TTTCAGTATT TAAATGAATT CAAGCAGCGA GAACTGCTAC GGAGTGGAGT GTTCCAGCAA 
CTGTCTGACA AGTGTAAATT ACAGCCTCCA GGAGCTACCG GACTTCAAAA TCTGAAGAGA 
GTGCTCGGTT ACTGCGAAGA TACCTATACC CGCTACAAAC TGGCCTTGGA AAACCAGTTC 
TATGATGTGA CCGATGTTTT AATGAGAGAC TCACAAACCC GCTGCTGCCT GACTGACATG 
CTGTCTCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753643.1
CDS	83..6754
Translation	MFVSVSSVICVRLLSLQGDDVLLLLSSSVLLHLHFSSEDNSLKLLSCLCLDLGFEATERISDVCLKSGLLFMLDTYGFVYVYDTVDGSPIFTMELPLRPENAALDPLNSICVSDDLGLFVVTTAASWALSVQLCEDAREISDHAVSAWHSDHVPDLHISGADEERLSSCHESVCTPRCTLRSDRSWDATLSSLCNEIMNSSDTFVCSKNPYLWPCNLPLKVPLSGIPVGEGWVRIVSEGLDDPLSLHIGSVTSFSAVLCLFGMDGLLNMIQWDMQSKEISSYPLGKAHFVHCSQEDACLVMSDRGLSLVVFSASQEEFLTRLMIHGSASTADTLCLLNKWGRCSVPIHTLEAGLDNRQLDTVDFFLKSKVSILNPLISEEPPDGIQAERYLQNVQDLLPALDLLVSYIRETDQETQSKHFSEQLLQLTLGFLNGQLHLLCALLSESSPALQQAVEILMGYITKLRRFMKKFMEQPQPVDVPGIEGQLHSWKNLSLEQIISDAILTNRIPEAQVFLRAEGHQACSLTWLKQEGLNLVYKCLLETRLQEARQLLRNMGFSVMNELKRICLHTSDRGVRTLLVDLLQREGCFSDQEQEDIQTVIHLEEVYCCPQKRTKESHKLLPHRLVQRLFTGSQEPEVLQAALTPSKNKSHMVLLYWAQGWDLETKEAILLPLRNSQVDMGFSHPRNLWCHLTLWHAWQRISSWIESLEKHGKTSNNDLSTWPLLTPEIVESCTLCCDYTKQKILDKLARMEIFVPSEQQNFEALVERMAVSRSMMPFPVSNPQCSTSENQDRHTHFVLLCAEQSLHYLLYTYLDYHRLNSQLCSALGNTTLHETLPWFAFLVQIRGVKDNSEDPSRMFNASLSNAHMVMPGYQPSMSSMLLEGHTLLALATNMYAPGGIDRVLEQHGESDSGHRNVDPQLLKMALAPYPKLKAALFSTHPATVSSPSDISLYHLLQALSPFQPTHFFTWQSVNTLAASDNSAVLPHCSSPALVSKFSVGEQLDVFFFLRSGRPSVAFATFLVFQLLRSKTPQQLIKQVAEDVYSLALSSFHLSSVLASCCCFFELLGLNSHKLRVDVNVAHMILSHSASLHEDPGVHKSQAQALAHRLCRLVENEREAAMDILHSLEEAVFSEECQNSSSLPTGSCLTIQQFCLLHSIPMSTRYLRSCAQQQDWLQLLVNTHTQEQVLCVAEKLSPALNNHIMLSLQDANSDAQISEYDLERSENDTVTASLYNILLGCQKTGQPGRTLLKECIKHRAPLLSVLAAYMQDTNQIACLCVWLVTSVDPANCAELTNMLSVASDHEWDLQDLTRIWEILLERKDSQTLYKAFSIFLEDCPLLLLLELYELCLQHKYYLEAGQKIDDFQRWMMNDDTEDTELSLLIPLPWLLQRASQLLQLLLLQTQTPYELRKVLQLLSDKAKPQLFGGIDVHKLSDLTSILQDHPIAISKELICHYSPEALQVECQRLIQMLLDKRIFSLAQRVAELAELPTDSIVIEEVLQDQCLLKEVGQWENSQSRVQYWRKCHQVFSATELSPAAASDFFCSQTLSLVAPECLLRDQTEFLAEQEMLFTLAGHWRSLSNTASIEVLEEIEQKIWKCRLAREVLSKHGAASQLCTLPSFASLTLEFSFSSLPALNSPSLLHISNLPPLGTPSSLLDREQTKALSSLVDCLLDESRVHEASRVCRYFLLPHYDLWLLLNCRALATGETVRDQLHPEIQAILGEGSERRESIWHQRKRLHSSSSLDTAMPPVPSDPVLLDLEILKDRCSHGKTYCQQLLCLYELSQDLGCSFSDISSRDPGEILRSLLSSQRPELTDRAQAVINFHGLSPQTVAQIVAEEGVRVWRAGPVEVYNASEIRQRFLQLVKLCQDPTLVGLTLLDYLENVPLTEQHCIIELLISAHDCFSLTCHLEGIRRVLQACRHLTESHLAANQEYSLMVRLLSGIGRYNEMVYVFDILHKEQHFEVLLRKQLDTKGGLQTALLEYIKRCHPGDSEKHNMTALCFSLHRDIGHNHEQAALIQLKLIQSRAWEYWMSELVELRSALMKALTLLIDAAESYSKDSCVRQSLRCARLTRLLTLQLHLLNNSHQTKLINLDRENLMEPILELPRFYQAVIVTEAYDIQPDWAEVLYHKVILAGDFQYLNEFKQRELLRSGVFQQLSDKCKLQPPGATGLQNLKRVLGYCEDTYTRYKLALENQFYDVTDVLMRDSQTRCCLTDMLSR

Target ORF information:

RefSeq Version	XM_031897783.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SPG11, spatacsin vesicle trafficking associated (spg11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897783.1

ORF Insert Sequence:

ATGTTTGTTA GTGTGTCCTC AGTCATCTGT GTTCGGCTAT TGTCCCTACA AGGAGATGAT 
GTTCTGCTGT TGCTGAGTAG CAGTGTCTTG TTGCATCTTC ACTTTTCTTC AGAAGATAAC 
AGTCTAAAAC TTTTATCCTG CTTATGCTTG GATTTGGGTT TCGAAGCAAC AGAGCGCATT 
TCAGATGTAT GTTTAAAAAG TGGGCTGTTG TTTATGCTGG ATACTTATGG TTTTGTATAT 
GTTTATGATA CTGTAGATGG CTCACCCATC TTCACCATGG AACTTCCACT AAGACCAGAG 
AATGCTGCTT TAGATCCACT CAACAGTATT TGTGTGTCTG ATGACCTAGG CTTATTTGTA 
GTCACCACTG CTGCTTCCTG GGCTCTCTCT GTGCAACTCT GTGAAGATGC CAGAGAGATC 
TCAGATCACG CAGTATCTGC CTGGCACAGT GATCATGTCC CAGATCTACA TATATCAGGT 
GCAGATGAAG AGAGACTGAG TAGTTGTCAT GAAAGTGTTT GTACCCCTAG ATGTACCTTA 
CGCTCAGACA GATCTTGGGA TGCCACACTG TCTTCACTAT GCAATGAGAT CATGAACTCT 
TCAGACACGT TTGTGTGCTC CAAGAACCCT TATCTATGGC CTTGTAACCT TCCTTTAAAA 
GTACCTCTCA GTGGGATCCC TGTGGGGGAA GGCTGGGTGA GGATTGTTTC TGAAGGGTTG 
GATGATCCTT TGAGTCTCCA CATCGGGTCT GTCACTTCCT TCAGTGCTGT ACTGTGTCTT 
TTTGGGATGG ATGGCTTACT TAACATGATA CAATGGGACA TGCAATCAAA GGAAATCTCT 
TCCTATCCCT TGGGAAAGGC TCACTTTGTT CACTGTTCTC AGGAAGATGC TTGCCTGGTG 
ATGTCAGATC GAGGGCTATC TCTAGTGGTG TTTTCTGCTT CACAAGAGGA ATTCCTGACC 
AGATTAATGA TTCATGGCAG TGCAAGTACA GCAGATACCT TATGTCTTCT AAATAAGTGG 
GGCCGTTGCT CCGTTCCCAT TCACACACTA GAGGCTGGTC TAGACAACAG ACAGTTAGAC 
ACTGTTGACT TTTTCCTGAA GAGCAAAGTA AGCATCCTAA ACCCTTTGAT CTCTGAGGAG 
CCTCCCGATG GCATCCAGGC GGAACGCTAT CTGCAAAATG TGCAGGATCT TCTGCCAGCT 
CTTGATCTTC TTGTCTCCTA CATCAGAGAA ACAGACCAGG AGACACAAAG CAAGCACTTC 
TCTGAGCAGT TGTTGCAGCT CACTCTTGGC TTCCTGAATG GACAACTTCA TCTGCTCTGT 
GCGCTGCTAT CTGAGTCAAG TCCTGCATTG CAGCAAGCTG TTGAGATTCT TATGGGTTAT 
ATAACCAAGC TACGCCGCTT CATGAAGAAA TTTATGGAAC AGCCACAACC TGTGGACGTC 
CCAGGTATTG AGGGACAGTT ACACTCTTGG AAGAATCTGT CATTGGAGCA AATAATCTCT 
GATGCCATTT TGACAAACCG CATTCCTGAG GCCCAGGTAT TCCTGCGTGC TGAGGGTCAT 
CAAGCTTGCA GCCTGACATG GTTGAAACAA GAAGGCCTTA ATCTGGTGTA CAAATGCCTG 
CTGGAGACAC GCCTGCAAGA GGCTCGTCAG CTTCTCCGAA ATATGGGCTT TTCTGTCATG 
AATGAGCTTA AGCGCATTTG TTTGCACACA TCAGATCGGG GCGTCAGGAC ATTGCTGGTA 
GATCTCCTGC AGAGAGAGGG GTGTTTTTCA GACCAAGAGC AGGAAGATAT TCAAACTGTG 
ATCCATTTGG AAGAAGTTTA CTGTTGTCCA CAAAAAAGGA CTAAAGAGAG TCATAAACTT 
CTGCCTCACA GGCTTGTACA GAGGCTTTTC ACTGGATCTC AGGAACCAGA AGTCCTACAA 
GCAGCTTTGA CACCTTCTAA GAACAAGTCA CACATGGTCC TCCTCTACTG GGCTCAGGGC 
TGGGATTTGG AAACAAAGGA AGCTATTTTA CTGCCCCTGA GGAACAGTCA AGTAGATATG 
GGTTTCTCAC ACCCAAGAAA TCTGTGGTGT CATCTTACTT TATGGCATGC ATGGCAGAGG 
ATTTCTTCAT GGATAGAATC TTTAGAAAAA CATGGAAAGA CAAGTAACAA TGATCTCTCA 
ACATGGCCTC TTCTGACTCC AGAAATAGTG GAGTCATGCA CCCTCTGTTG TGACTATACA 
AAGCAGAAGA TCCTGGACAA GTTAGCAAGA ATGGAAATAT TTGTCCCTTC TGAGCAGCAG 
AATTTTGAGG CCCTTGTGGA ACGTATGGCA GTTAGTAGGA GCATGATGCC GTTTCCTGTC 
TCCAACCCTC AGTGCAGCAC CTCTGAGAAT CAAGATCGTC ACACCCACTT CGTTTTGCTG 
TGTGCTGAGC AGAGTCTTCA CTACCTATTG TACACATATC TGGATTATCA CAGGTTGAAT 
TCTCAGCTTT GCTCTGCTTT GGGCAATACA ACCTTGCATG AAACCTTGCC TTGGTTTGCA 
TTTTTGGTAC AGATACGAGG TGTCAAAGAC AACTCTGAAG ATCCAAGCCG CATGTTTAAT 
GCTAGCCTCT CCAATGCCCA TATGGTGATG CCAGGGTACC AGCCTAGTAT GAGCAGCATG 
CTGCTGGAGG GACACACATT GCTCGCCCTT GCAACCAATA TGTATGCACC TGGTGGCATT 
GACAGGGTGT TGGAGCAGCA TGGAGAGTCA GATTCTGGTC ATAGAAATGT AGACCCTCAG 
CTTCTTAAAA TGGCTTTAGC TCCATATCCG AAGCTCAAGG CAGCTCTCTT CAGTACACAC 
CCAGCTACTG TCAGTTCTCC CTCTGACATT TCACTTTACC ATCTCCTACA GGCTCTCTCC 
CCATTTCAGC CTACACACTT CTTCACATGG CAGTCGGTAA ATACCCTTGC TGCAAGTGAC 
AACAGTGCAG TGCTCCCACA TTGCTCAAGT CCAGCCTTGG TCAGCAAGTT CTCTGTGGGT 
GAGCAACTAG ATGTCTTCTT CTTCCTGCGG AGCGGGCGAC CATCAGTCGC ATTCGCTACC 
TTTCTGGTGT TTCAGCTACT AAGAAGCAAA ACCCCCCAAC AGCTAATCAA GCAAGTTGCT 
GAGGATGTTT ATTCCCTGGC ACTGTCTTCA TTTCATCTCT CCTCTGTGCT TGCTTCATGC 
TGCTGCTTCT TTGAGTTACT GGGTCTCAAC AGCCACAAGC TTCGAGTGGA TGTGAACGTT 
GCACACATGA TACTAAGTCA CTCAGCTTCT CTGCATGAGG ATCCAGGGGT ACACAAATCC 
CAGGCACAAG CTTTAGCTCA CAGACTTTGC AGACTGGTGG AGAATGAGAG AGAAGCAGCC 
ATGGATATTT TACATAGCCT GGAGGAAGCT GTATTTTCTG AAGAGTGTCA AAACAGCTCA 
TCTCTACCCA CTGGCTCCTG TTTAACTATT CAGCAGTTCT GCCTCCTCCA TTCTATTCCC 
ATGAGCACTA GGTATTTGAG GAGCTGCGCA CAGCAACAAG ACTGGCTGCA GTTACTTGTG 
AACACACACA CTCAGGAACA GGTCCTATGT GTAGCTGAAA AGTTGAGCCC GGCACTTAAT 
AATCACATAA TGCTATCATT GCAAGATGCA AATTCAGATG CACAAATCTC TGAATATGAC 
TTGGAAAGAT CTGAAAATGA TACAGTGACT GCCAGTTTGT ACAATATATT GCTTGGGTGC 
CAGAAGACTG GGCAGCCAGG CCGCACACTT CTAAAGGAAT GTATTAAGCA CAGAGCTCCA 
TTGCTGAGTG TGCTTGCTGC ATATATGCAG GACACCAACC AAATTGCTTG CCTGTGTGTG 
TGGCTTGTAA CCTCTGTAGA TCCGGCCAAT TGTGCAGAAC TCACAAATAT GCTTTCGGTT 
GCTTCAGATC ATGAGTGGGA CTTGCAAGAT CTGACACGGA TTTGGGAAAT CTTGCTGGAG 
AGAAAAGATA GCCAGACTTT GTACAAAGCA TTCAGCATCT TTTTAGAGGA CTGTCCCCTT 
CTTCTCTTAC TTGAACTGTA TGAGCTTTGC CTGCAACATA AATACTATCT GGAGGCTGGA 
CAGAAAATAG ATGATTTCCA AAGATGGATG ATGAACGATG ACACTGAAGA TACAGAGTTA 
TCGCTTCTCA TTCCACTGCC CTGGTTGCTA CAAAGAGCTT CTCAACTTCT GCAGCTCCTT 
CTCTTGCAGA CCCAGACTCC ATATGAATTG CGGAAGGTTT TGCAGCTTCT GAGTGACAAA 
GCTAAACCAC AGTTATTTGG CGGAATAGAT GTTCATAAGC TGAGTGATCT AACCTCTATT 
CTTCAAGACC ACCCTATTGC CATTAGCAAA GAATTAATAT GCCACTATAG TCCTGAAGCC 
CTGCAGGTTG AGTGTCAAAG GCTTATACAG ATGCTGTTGG ACAAAAGGAT TTTTTCTCTT 
GCACAAAGAG TAGCAGAACT AGCAGAATTG CCCACTGACA GTATTGTCAT AGAAGAGGTG 
TTGCAGGACC AATGTCTCTT AAAAGAGGTT GGGCAGTGGG AGAATTCTCA AAGCCGTGTA 
CAGTACTGGA GAAAATGCCA CCAGGTGTTT AGCGCCACGG AGCTCTCCCC AGCTGCAGCG 
TCTGACTTCT TCTGCTCTCA GACTCTTTCT CTTGTAGCGC CTGAATGCTT GCTTAGGGAT 
CAGACAGAGT TTTTGGCAGA GCAGGAGATG CTGTTTACCC TTGCTGGGCA CTGGAGGTCC 
CTAAGTAACA CTGCTTCTAT AGAAGTCCTG GAGGAAATTG AGCAGAAGAT TTGGAAATGT 
CGCCTGGCAA GAGAAGTATT GAGTAAACAT GGAGCTGCTA GTCAACTCTG TACTCTGCCT 
TCATTTGCTA GCTTGACCCT GGAGTTTTCA TTCTCATCTT TGCCTGCACT AAACTCTCCT 
TCGCTTCTTC ATATCTCTAA TTTGCCACCG CTTGGTACTC CTAGCAGTTT GTTGGACAGA 
GAACAGACCA AAGCATTGAG CTCTTTGGTT GACTGCCTGT TGGATGAGAG CCGTGTGCAC 
GAGGCCAGTC GGGTATGCAG GTACTTTCTG CTACCTCATT ATGATCTTTG GCTACTGCTG 
AACTGCAGGG CACTAGCAAC AGGAGAGACC GTAAGGGACC AACTGCATCC GGAAATTCAA 
GCTATCCTCG GTGAAGGCAG CGAACGCCGG GAAAGCATAT GGCACCAGAG GAAAAGACTG 
CACAGCTCCT CCTCTCTGGA CACCGCCATG CCCCCTGTAC CCAGTGATCC TGTGCTCTTA 
GATCTGGAAA TTCTGAAGGA CAGGTGCTCC CATGGCAAAA CCTACTGCCA ACAGCTGCTG 
TGTCTTTACG AGTTAAGCCA GGACCTGGGA TGTTCCTTCT CAGATATCTC CTCTCGAGAT 
CCCGGCGAGA TTCTACGCTC TCTGCTTTCT TCTCAGCGTC CCGAGCTCAC AGACAGAGCA 
CAAGCAGTCA TCAACTTCCA CGGACTGTCG CCGCAAACAG TGGCACAGAT TGTGGCAGAA 
GAGGGTGTGA GAGTCTGGCG GGCAGGGCCC GTGGAAGTTT ATAATGCCTC TGAAATAAGG 
CAGCGCTTTT TGCAGCTTGT AAAACTTTGC CAGGACCCAA CTCTGGTTGG ATTAACGCTA 
CTTGACTATC TAGAAAATGT GCCACTGACT GAGCAACACT GCATTATTGA ACTCCTGATT 
TCTGCCCACG ATTGCTTCAG TTTAACGTGC CATCTTGAAG GAATTCGACG GGTGCTTCAG 
GCATGTCGAC ACCTAACCGA GAGTCACTTG GCGGCTAACC AAGAATACAG CCTTATGGTT 
CGCCTACTCT CTGGGATTGG TCGTTATAAT GAGATGGTTT ATGTGTTTGA CATTCTGCAC 
AAGGAACAGC ACTTTGAGGT TCTGCTGAGG AAACAGCTAG ATACAAAGGG GGGACTGCAG 
ACTGCTCTTT TGGAATACAT TAAGCGCTGC CATCCAGGAG ACAGCGAGAA GCACAACATG 
ACTGCGCTCT GCTTCAGTTT GCACCGTGAC ATTGGCCACA ACCATGAGCA GGCAGCTTTG 
ATCCAGCTCA AACTCATCCA GTCTAGGGCA TGGGAATACT GGATGTCTGA ATTGGTGGAG 
TTGAGATCTG CACTAATGAA AGCACTTACC CTCCTAATAG ATGCAGCTGA GAGCTACTCA 
AAGGATTCCT GCGTCCGTCA GTCTCTGCGC TGTGCTCGCC TTACCCGTCT GTTGACTCTG 
CAGCTTCATC TGCTAAACAA CAGTCATCAA ACTAAATTAA TCAACCTGGA TAGAGAGAAC 
TTGATGGAGC CAATACTGGA ACTGCCACGT TTCTACCAGG CAGTCATTGT TACAGAGGCC 
TATGATATAC AGCCAGACTG GGCAGAGGTT TTATATCACA AGGTGATTCT TGCTGGAGAC 
TTTCAGTATT TAAATGAATT CAAGCAGCGA GAACTGCTAC GGAGTGGAGT GTTCCAGCAA 
CTGTCTGACA AGTGTAAATT ACAGCCTCCA GGAGCTACCG GACTTCAAAA TCTGAAGAGA 
GTGCTCGGTT ACTGCGAAGA TACCTATACC CGCTACAAAC TGGCCTTGGA AAACCAGTTC 
TATGATGTGA CCGATGTTTT AATGAGAGAC TCACAAACCC GCTGCTGCCT GACTGACATG 
CTGTCTCGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( XM_012958756.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814210.1 Xenopus tropicalis spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X1, mRNA.

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CloneID	OXa25973
Clone ID Related Accession (Same CDS sequence)	XM_012958756.1 , NM_203738.3
Accession Version	XM_012958756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGACTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGTAGATA TGGGTTTCTC ACACCCAAGA 
AATCTGTGGT GTCATCTTAC TTTATGGCAT GCATGGCAGA GGATTTCTTC ATGGATAGAA 
TCTTTAGAAA AACATGGAAA GACAAGTAAC AATGATCTCT CAACATGGCC TCTTCTGACT 
CCAGAAATAG TGGAGTCATG CACCCTCTGT TGTGACTATA CAAAGCAGAA GATCCTGGAC 
AAGTTAGCAA GAATGGAAAT ATTTGTCCCT TCTGAGCAGC AGAATTTTGA GGCCCTTGTG 
GAACGTATGG CAGTTAGTAG GAGCATGATG CCGTTTCCTG TCTCCAACCC TCAGTGCAGC 
ACCTCTGAGA ATCAAGATCG TCACACCCAC TTCGTTTTGC TGTGTGCTGA GCAGAGTCTT 
CACTACCTAT TGTACACATA TCTGGATTAT CACAGGTTGA ATTCTCAGCT TTGCTCTGCT 
TTGGGCAATA CAACCTTGCA TGAAACCTTG CCTTGGTTTG CATTTTTGGT ACAGATACGA 
GGTGTCAAAG ACAACTCTGA AGATCCAAGC CGCATGTTTA ATGCTAGCCT CTCCAATGCC 
CATATGGTGA TGCCAGGGTA CCAGCCTAGT ATGAGCAGCA TGCTGCTGGA GGGACACACA 
TTGCTCGCCC TTGCAACCAA TATGTATGCA CCTGGTGGCA TTGACAGGGT GTTGGAGCAG 
CATGGAGAGT CAGATTCTGG TCATAGAAAT GTAGACCCTC AGCTTCTTAA AATGGCTTTA 
GCTCCATATC CGAAGCTCAA GGCAGCTCTC TTCAGTACAC ACCCAGCTAC TGTCAGTTCT 
CCCTCTGACA TTTCACTTTA CCATCTCCTA CAGGCTCTCT CCCCATTTCA GCCTACACAC 
TTCTTCACAT GGCAGTCGGT AAATACCCTT GCTGCAAGTG ACAACAGTGC AGTGCTCCCA 
CATTGCTCAA GTCCAGCCTT GGTCAGCAAG TTCTCTGTGG GTGAGCAACT AGATGTCTTC 
TTCTTCCTGC GGAGCGGGCG ACCATCAGTC GCATTCGCTA CCTTTCTGGT GTTTCAGCTA 
CTAAGAAGCA AAACCCCCCA ACAGCTAATC AAGCAAGTTG CTGAGGATGT TTATTCCCTG 
GCACTGTCTT CATTTCATCT CTCCTCTGTG CTTGCTTCAT GCTGCTGCTT CTTTGAGTTA 
CTGGGTCTCA ACAGCCACAA GCTTCGAGTG GATGTGAACG TTGCACACAT GATACTAAGT 
CACTCAGCTT CTCTGCATGA GGATCCAGGG GTACACAAAT CCCAGGCACA AGCTTTAGCT 
CACAGACTTT GCAGACTGGT GGAGAATGAG AGAGAAGCAG CCATGGATAT TTTACATAGC 
CTGGAGGAAG CTGTATTTTC TGAAGAGTGT CAAAACAGCT CATCTCTACC CACTGGCTCC 
TGTTTAACTA TTCAGCAGTT CTGCCTCCTC CATTCTATTC CCATGAGCAC TAGGTATTTG 
AGGAGCTGCG CACAGCAACA AGACTGGCTG CAGTTACTTG TGAACACACA CACTCAGGAA 
CAGGTCCTAT GTGTAGCTGA AAAGTTGAGC CCGGCACTTA ATAATCACAT AATGCTATCA 
TTGCAAGATG CAAATTCAGA TGCACAAATC TCTGAATATG ACTTGGAAAG ATCTGAAAAT 
GATACAGTGA CTGCCAGTTT GTACAATATA TTGCTTGGGT GCCAGAAGAC TGGGCAGCCA 
GGCCGCACAC TTCTAAAGGA ATGTATTAAG CACAGAGCTC CATTGCTGAG TGTGCTTGCT 
GCATATATGC AGGACACCAA CCAAATTGCT TGCCTGTGTG TGTGGCTTGT AACCTCTGTA 
GATCCGGCCA ATTGTGCAGA ACTCACAAAT ATGCTTTCGG TTGCTTCAGA TCATGAGTGG 
GACTTGCAAG ATCTGACACG GATTTGGGAA ATCTTGCTGG AGAGAAAAGA TAGCCAGACT 
TTGTACAAAG CATTCAGCAT CTTTTTAGAG GACTGTCCCC TTCTTCTCTT ACTTGAACTG 
TATGAGCTTT GCCTGCAACA TAAATACTAT CTGGAGGCTG GACAGAAAAT AGATGATTTC 
CAAAGATGGA TGATGAACGA TGACACTGAA GATACAGAGT TATCGCTTCT CATTCCACTG 
CCCTGGTTGC TACAAAGAGC TTCTCAACTT CTGCAGCTCC TTCTCTTGCA GACCCAGACT 
CCATATGAAT TGCGGAAGGT TTTGCAGCTT CTGAGTGACA AAGCTAAACC ACAGTTATTT 
GGCGGAATAG ATGTTCATAA GCTGAGTGAT CTAACCTCTA TTCTTCAAGA CCACCCTATT 
GCCATTAGCA AAGAATTAAT ATGCCACTAT AGTCCTGAAG CCCTGCAGGT TGAGTGTCAA 
AGGCTTATAC AGATGCTGTT GGACAAAAGG ATTTTTTCTC TTGCACAAAG AGTAGCAGAA 
CTAGCAGAAT TGCCCACTGA CAGTATTGTC ATAGAAGAGG TGTTGCAGGA CCAATGTCTC 
TTAAAAGAGG TTGGGCAGTG GGAGAATTCT CAAAGCCGTG TACAGTACTG GAGAAAATGC 
CACCAGGTGT TTAGCGCCAC GGAGCTCTCC CCAGCTGCAG CGTCTGACTT CTTCTGCTCT 
CAGACTCTTT CTCTTGTAGC GCCTGAATGC TTGCTTAGGG ATCAGACAGA GTTTTTGGCA 
GAGCAGGAGA TGCTGTTTAC CCTTGCTGGG CACTGGAGGT CCCTAAGTAA CACTGCTTCT 
ATAGAAGTCC TGGAGGAAAT TGAGCAGAAG ATTTGGAAAT GTCGCCTGGC AAGAGAAGTA 
TTGAGTAAAC ATGGAGCTGC TAGTCAACTC TGTACTCTGC CTTCATTTGC TAGCTTGACC 
CTGGAGTTTT CATTCTCATC TTTGCCTGCA CTAAACTCTC CTTCGCTTCT TCATATCTCT 
AATTTGCCAC CGCTTGGTAC TCCTAGCAGT TTGTTGGACA GAGAACAGAC CAAAGCATTG 
AGCTCTTTGG TTGACTGCCT GTTGGATGAG AGCCGTGTGC ACGAGGCCAG TCGGGTATGC 
AGGTACTTTC TGCTACCTCA TTATGATCTT TGGCTACTGC TGAACTGCAG GGCACTAGCA 
ACAGGAGAGA CCGTAAGGGA CCAACTGCAT CCGGAAATTC AAGCTATCCT CGGTGAAGGC 
AGCGAACGCC GGGAAAGCAT ATGGCACCAG AGGAAAAGAC TGCACAGCTC CTCCTCTCTG 
GACACCGCCA TGCCCCCTGT ACCCAGTGAT CCTGTGCTCT TAGATCTGGA AATTCTGAAG 
GACAGGTGCT CCCATGGCAA AACCTACTGC CAACAGCTGC TGTGTCTTTA CGAGTTAAGC 
CAGGACCTGG GATGTTCCTT CTCAGATATC TCCTCTCGAG ATCCCGGCGA GATTCTACGC 
TCTCTGCTTT CTTCTCAGCG TCCCGAGCTC ACAGACAGAG CACAAGCAGT CATCAACTTC 
CACGGACTGT CGCCGCAAAC AGTGGCACAG ATTGTGGCAG AAGAGGGTGT GAGAGTCTGG 
CGGGCAGGGC CCGTGGAAGT TTATAATGCC TCTGAAATAA GGCAGCGCTT TTTGCAGCTT 
GTAAAACTTT GCCAGGACCC AACTCTGGTT GGATTAACGC TACTTGACTA TCTAGAAAAT 
GTGCCACTGA CTGAGCAACA CTGCATTATT GAACTCCTGA TTTCTGCCCA CGATTGCTTC 
AGTTTAACGT GCCATCTTGA AGGAATTCGA CGGGTGCTTC AGGCATGTCG ACACCTAACC 
GAGAGTCACT TGGCGGCTAA CCAAGAATAC AGCCTTATGG TTCGCCTACT CTCTGGGATT 
GGTCGTTATA ATGAGATGGT TTATGTGTTT GACATTCTGC ACAAGGAACA GCACTTTGAG 
GTTCTGCTGA GGAAACAGCT AGATACAAAG GGGGGACTGC AGACTGCTCT TTTGGAATAC 
ATTAAGCGCT GCCATCCAGG AGACAGCGAG AAGCACAACA TGACTGCGCT CTGCTTCAGT 
TTGCACCGTG ACATTGGCCA CAACCATGAG CAGGCAGCTT TGATCCAGCT CAAACTCATC 
CAGTCTAGGG CATGGGAATA CTGGATGTCT GAATTGGTGG AGTTGAGATC TGCACTAATG 
AAAGCACTTA CCCTCCTAAT AGATGCAGCT GAGAGCTACT CAAAGGATTC CTGCGTCCGT 
CAGTCTCTGC GCTGTGCTCG CCTTACCCGT CTGTTGACTC TGCAGCTTCA TCTGCTAAAC 
AACAGTCATC AAACTAAATT AATCAACCTG GATAGAGAGA ACTTGATGGA GCCAATACTG 
GAACTGCCAC GTTTCTACCA GGCAGTCATT GTTACAGAGG CCTATGATAT ACAGCCAGAC 
TGGGCAGAGG TTTTATATCA CAAGGTGATT CTTGCTGGAG ACTTTCAGTA TTTAAATGAA 
TTCAAGCAGC GAGAACTGCT ACGGAGTGGA GTGTTCCAGC AACTGTCTGA CAAGTGTAAA 
TTACAGCCTC CAGGAGCTAC CGGACTTCAA AATCTGAAGA GAGTGCTCGG TTACTGCGAA 
GATACCTATA CCCGCTACAA ACTGGCCTTG GAAAACCAGT TCTATGATGT GACCGATGTT 
TTAATGAGAG ACTCACAAAC CCGCTGCTGC CTGACTGACA TGCTGTCTCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814210.1
CDS	215..7288
Translation	MGRRILQSCKMNLNILLVPGLTLPEGTNPPVRVSLTRQKHPTLCSLCPQGELHLTALPYEGFHTTHTQLEGLFSHFLWENADGNEEPSDNIPRLLTLSGSNEVAVHGLHVADGHLNVTTIFLCDEDTLRKLCADNHVCVSSVICVRLLSLQGDDVLLLLSSSVLLHLHFSSEDNSLKLLSCLCLDLGFEATERISDVCLKSGLLFMLDTYGFVYVYDTVDGSPIFTMELPLRPENAALDPLNSICVSDDLGLFVVTTAASWALSVQLCEDAREISDHAVSAWHSDHVPDLHISGADEERLSSCHESVCTPRCTLRSDRSWDATLSSLCNEIMNSSDTFVCSKNPYLWPCNLPLKVPLSGIPVGEGWVRIVSEGLDDPLSLHIGSVTSFSAVLCLFGMDGLLNMIQWDMQSKEISSYPLGKAHFVHCSQEDACLVMSDRGLSLVVFSASQEEFLTRLMIHGSASTADTLCLLNKWGRCSVPIHTLEAGLDNRQLDTVDFFLKSKVSILNPLISEEPPDGIQAERYLQNVQDLLPALDLLVSYIRETDQETQSKHFSEQLLQLTLGFLNGQLHLLCALLSESSPALQQAVEILMGYITKLRRFMKKFMEQPQPVDVPGIEGQLHSWKNLSLEQIISDAILTNRIPEAQVFLRAEGHQACSLTWLKQEGLNLVYKCLLETRLQETRQLLRNMGFSVMNELKRICLHTSDRGVRTLLVDLLQREGCFSDQEQEDIQTVIHLEEVYCCPQKRTKESHKLLPHRLVQRLFTGSQEPEVLQAALTPSKNKSHMVLLYWAQGWDLETKEAILLPLRNSQVDMGFSHPRNLWCHLTLWHAWQRISSWIESLEKHGKTSNNDLSTWPLLTPEIVESCTLCCDYTKQKILDKLARMEIFVPSEQQNFEALVERMAVSRSMMPFPVSNPQCSTSENQDRHTHFVLLCAEQSLHYLLYTYLDYHRLNSQLCSALGNTTLHETLPWFAFLVQIRGVKDNSEDPSRMFNASLSNAHMVMPGYQPSMSSMLLEGHTLLALATNMYAPGGIDRVLEQHGESDSGHRNVDPQLLKMALAPYPKLKAALFSTHPATVSSPSDISLYHLLQALSPFQPTHFFTWQSVNTLAASDNSAVLPHCSSPALVSKFSVGEQLDVFFFLRSGRPSVAFATFLVFQLLRSKTPQQLIKQVAEDVYSLALSSFHLSSVLASCCCFFELLGLNSHKLRVDVNVAHMILSHSASLHEDPGVHKSQAQALAHRLCRLVENEREAAMDILHSLEEAVFSEECQNSSSLPTGSCLTIQQFCLLHSIPMSTRYLRSCAQQQDWLQLLVNTHTQEQVLCVAEKLSPALNNHIMLSLQDANSDAQISEYDLERSENDTVTASLYNILLGCQKTGQPGRTLLKECIKHRAPLLSVLAAYMQDTNQIACLCVWLVTSVDPANCAELTNMLSVASDHEWDLQDLTRIWEILLERKDSQTLYKAFSIFLEDCPLLLLLELYELCLQHKYYLEAGQKIDDFQRWMMNDDTEDTELSLLIPLPWLLQRASQLLQLLLLQTQTPYELRKVLQLLSDKAKPQLFGGIDVHKLSDLTSILQDHPIAISKELICHYSPEALQVECQRLIQMLLDKRIFSLAQRVAELAELPTDSIVIEEVLQDQCLLKEVGQWENSQSRVQYWRKCHQVFSATELSPAAASDFFCSQTLSLVAPECLLRDQTEFLAEQEMLFTLAGHWRSLSNTASIEVLEEIEQKIWKCRLAREVLSKHGAASQLCTLPSFASLTLEFSFSSLPALNSPSLLHISNLPPLGTPSSLLDREQTKALSSLVDCLLDESRVHEASRVCRYFLLPHYDLWLLLNCRALATGETVRDQLHPEIQAILGEGSERRESIWHQRKRLHSSSSLDTAMPPVPSDPVLLDLEILKDRCSHGKTYCQQLLCLYELSQDLGCSFSDISSRDPGEILRSLLSSQRPELTDRAQAVINFHGLSPQTVAQIVAEEGVRVWRAGPVEVYNASEIRQRFLQLVKLCQDPTLVGLTLLDYLENVPLTEQHCIIELLISAHDCFSLTCHLEGIRRVLQACRHLTESHLAANQEYSLMVRLLSGIGRYNEMVYVFDILHKEQHFEVLLRKQLDTKGGLQTALLEYIKRCHPGDSEKHNMTALCFSLHRDIGHNHEQAALIQLKLIQSRAWEYWMSELVELRSALMKALTLLIDAAESYSKDSCVRQSLRCARLTRLLTLQLHLLNNSHQTKLINLDRENLMEPILELPRFYQAVIVTEAYDIQPDWAEVLYHKVILAGDFQYLNEFKQRELLRSGVFQQLSDKCKLQPPGATGLQNLKRVLGYCEDTYTRYKLALENQFYDVTDVLMRDSQTRCCLTDMLSR

Target ORF information:

RefSeq Version	XM_012958756.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958756.1

ORF Insert Sequence:

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGACTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGTAGATA TGGGTTTCTC ACACCCAAGA 
AATCTGTGGT GTCATCTTAC TTTATGGCAT GCATGGCAGA GGATTTCTTC ATGGATAGAA 
TCTTTAGAAA AACATGGAAA GACAAGTAAC AATGATCTCT CAACATGGCC TCTTCTGACT 
CCAGAAATAG TGGAGTCATG CACCCTCTGT TGTGACTATA CAAAGCAGAA GATCCTGGAC 
AAGTTAGCAA GAATGGAAAT ATTTGTCCCT TCTGAGCAGC AGAATTTTGA GGCCCTTGTG 
GAACGTATGG CAGTTAGTAG GAGCATGATG CCGTTTCCTG TCTCCAACCC TCAGTGCAGC 
ACCTCTGAGA ATCAAGATCG TCACACCCAC TTCGTTTTGC TGTGTGCTGA GCAGAGTCTT 
CACTACCTAT TGTACACATA TCTGGATTAT CACAGGTTGA ATTCTCAGCT TTGCTCTGCT 
TTGGGCAATA CAACCTTGCA TGAAACCTTG CCTTGGTTTG CATTTTTGGT ACAGATACGA 
GGTGTCAAAG ACAACTCTGA AGATCCAAGC CGCATGTTTA ATGCTAGCCT CTCCAATGCC 
CATATGGTGA TGCCAGGGTA CCAGCCTAGT ATGAGCAGCA TGCTGCTGGA GGGACACACA 
TTGCTCGCCC TTGCAACCAA TATGTATGCA CCTGGTGGCA TTGACAGGGT GTTGGAGCAG 
CATGGAGAGT CAGATTCTGG TCATAGAAAT GTAGACCCTC AGCTTCTTAA AATGGCTTTA 
GCTCCATATC CGAAGCTCAA GGCAGCTCTC TTCAGTACAC ACCCAGCTAC TGTCAGTTCT 
CCCTCTGACA TTTCACTTTA CCATCTCCTA CAGGCTCTCT CCCCATTTCA GCCTACACAC 
TTCTTCACAT GGCAGTCGGT AAATACCCTT GCTGCAAGTG ACAACAGTGC AGTGCTCCCA 
CATTGCTCAA GTCCAGCCTT GGTCAGCAAG TTCTCTGTGG GTGAGCAACT AGATGTCTTC 
TTCTTCCTGC GGAGCGGGCG ACCATCAGTC GCATTCGCTA CCTTTCTGGT GTTTCAGCTA 
CTAAGAAGCA AAACCCCCCA ACAGCTAATC AAGCAAGTTG CTGAGGATGT TTATTCCCTG 
GCACTGTCTT CATTTCATCT CTCCTCTGTG CTTGCTTCAT GCTGCTGCTT CTTTGAGTTA 
CTGGGTCTCA ACAGCCACAA GCTTCGAGTG GATGTGAACG TTGCACACAT GATACTAAGT 
CACTCAGCTT CTCTGCATGA GGATCCAGGG GTACACAAAT CCCAGGCACA AGCTTTAGCT 
CACAGACTTT GCAGACTGGT GGAGAATGAG AGAGAAGCAG CCATGGATAT TTTACATAGC 
CTGGAGGAAG CTGTATTTTC TGAAGAGTGT CAAAACAGCT CATCTCTACC CACTGGCTCC 
TGTTTAACTA TTCAGCAGTT CTGCCTCCTC CATTCTATTC CCATGAGCAC TAGGTATTTG 
AGGAGCTGCG CACAGCAACA AGACTGGCTG CAGTTACTTG TGAACACACA CACTCAGGAA 
CAGGTCCTAT GTGTAGCTGA AAAGTTGAGC CCGGCACTTA ATAATCACAT AATGCTATCA 
TTGCAAGATG CAAATTCAGA TGCACAAATC TCTGAATATG ACTTGGAAAG ATCTGAAAAT 
GATACAGTGA CTGCCAGTTT GTACAATATA TTGCTTGGGT GCCAGAAGAC TGGGCAGCCA 
GGCCGCACAC TTCTAAAGGA ATGTATTAAG CACAGAGCTC CATTGCTGAG TGTGCTTGCT 
GCATATATGC AGGACACCAA CCAAATTGCT TGCCTGTGTG TGTGGCTTGT AACCTCTGTA 
GATCCGGCCA ATTGTGCAGA ACTCACAAAT ATGCTTTCGG TTGCTTCAGA TCATGAGTGG 
GACTTGCAAG ATCTGACACG GATTTGGGAA ATCTTGCTGG AGAGAAAAGA TAGCCAGACT 
TTGTACAAAG CATTCAGCAT CTTTTTAGAG GACTGTCCCC TTCTTCTCTT ACTTGAACTG 
TATGAGCTTT GCCTGCAACA TAAATACTAT CTGGAGGCTG GACAGAAAAT AGATGATTTC 
CAAAGATGGA TGATGAACGA TGACACTGAA GATACAGAGT TATCGCTTCT CATTCCACTG 
CCCTGGTTGC TACAAAGAGC TTCTCAACTT CTGCAGCTCC TTCTCTTGCA GACCCAGACT 
CCATATGAAT TGCGGAAGGT TTTGCAGCTT CTGAGTGACA AAGCTAAACC ACAGTTATTT 
GGCGGAATAG ATGTTCATAA GCTGAGTGAT CTAACCTCTA TTCTTCAAGA CCACCCTATT 
GCCATTAGCA AAGAATTAAT ATGCCACTAT AGTCCTGAAG CCCTGCAGGT TGAGTGTCAA 
AGGCTTATAC AGATGCTGTT GGACAAAAGG ATTTTTTCTC TTGCACAAAG AGTAGCAGAA 
CTAGCAGAAT TGCCCACTGA CAGTATTGTC ATAGAAGAGG TGTTGCAGGA CCAATGTCTC 
TTAAAAGAGG TTGGGCAGTG GGAGAATTCT CAAAGCCGTG TACAGTACTG GAGAAAATGC 
CACCAGGTGT TTAGCGCCAC GGAGCTCTCC CCAGCTGCAG CGTCTGACTT CTTCTGCTCT 
CAGACTCTTT CTCTTGTAGC GCCTGAATGC TTGCTTAGGG ATCAGACAGA GTTTTTGGCA 
GAGCAGGAGA TGCTGTTTAC CCTTGCTGGG CACTGGAGGT CCCTAAGTAA CACTGCTTCT 
ATAGAAGTCC TGGAGGAAAT TGAGCAGAAG ATTTGGAAAT GTCGCCTGGC AAGAGAAGTA 
TTGAGTAAAC ATGGAGCTGC TAGTCAACTC TGTACTCTGC CTTCATTTGC TAGCTTGACC 
CTGGAGTTTT CATTCTCATC TTTGCCTGCA CTAAACTCTC CTTCGCTTCT TCATATCTCT 
AATTTGCCAC CGCTTGGTAC TCCTAGCAGT TTGTTGGACA GAGAACAGAC CAAAGCATTG 
AGCTCTTTGG TTGACTGCCT GTTGGATGAG AGCCGTGTGC ACGAGGCCAG TCGGGTATGC 
AGGTACTTTC TGCTACCTCA TTATGATCTT TGGCTACTGC TGAACTGCAG GGCACTAGCA 
ACAGGAGAGA CCGTAAGGGA CCAACTGCAT CCGGAAATTC AAGCTATCCT CGGTGAAGGC 
AGCGAACGCC GGGAAAGCAT ATGGCACCAG AGGAAAAGAC TGCACAGCTC CTCCTCTCTG 
GACACCGCCA TGCCCCCTGT ACCCAGTGAT CCTGTGCTCT TAGATCTGGA AATTCTGAAG 
GACAGGTGCT CCCATGGCAA AACCTACTGC CAACAGCTGC TGTGTCTTTA CGAGTTAAGC 
CAGGACCTGG GATGTTCCTT CTCAGATATC TCCTCTCGAG ATCCCGGCGA GATTCTACGC 
TCTCTGCTTT CTTCTCAGCG TCCCGAGCTC ACAGACAGAG CACAAGCAGT CATCAACTTC 
CACGGACTGT CGCCGCAAAC AGTGGCACAG ATTGTGGCAG AAGAGGGTGT GAGAGTCTGG 
CGGGCAGGGC CCGTGGAAGT TTATAATGCC TCTGAAATAA GGCAGCGCTT TTTGCAGCTT 
GTAAAACTTT GCCAGGACCC AACTCTGGTT GGATTAACGC TACTTGACTA TCTAGAAAAT 
GTGCCACTGA CTGAGCAACA CTGCATTATT GAACTCCTGA TTTCTGCCCA CGATTGCTTC 
AGTTTAACGT GCCATCTTGA AGGAATTCGA CGGGTGCTTC AGGCATGTCG ACACCTAACC 
GAGAGTCACT TGGCGGCTAA CCAAGAATAC AGCCTTATGG TTCGCCTACT CTCTGGGATT 
GGTCGTTATA ATGAGATGGT TTATGTGTTT GACATTCTGC ACAAGGAACA GCACTTTGAG 
GTTCTGCTGA GGAAACAGCT AGATACAAAG GGGGGACTGC AGACTGCTCT TTTGGAATAC 
ATTAAGCGCT GCCATCCAGG AGACAGCGAG AAGCACAACA TGACTGCGCT CTGCTTCAGT 
TTGCACCGTG ACATTGGCCA CAACCATGAG CAGGCAGCTT TGATCCAGCT CAAACTCATC 
CAGTCTAGGG CATGGGAATA CTGGATGTCT GAATTGGTGG AGTTGAGATC TGCACTAATG 
AAAGCACTTA CCCTCCTAAT AGATGCAGCT GAGAGCTACT CAAAGGATTC CTGCGTCCGT 
CAGTCTCTGC GCTGTGCTCG CCTTACCCGT CTGTTGACTC TGCAGCTTCA TCTGCTAAAC 
AACAGTCATC AAACTAAATT AATCAACCTG GATAGAGAGA ACTTGATGGA GCCAATACTG 
GAACTGCCAC GTTTCTACCA GGCAGTCATT GTTACAGAGG CCTATGATAT ACAGCCAGAC 
TGGGCAGAGG TTTTATATCA CAAGGTGATT CTTGCTGGAG ACTTTCAGTA TTTAAATGAA 
TTCAAGCAGC GAGAACTGCT ACGGAGTGGA GTGTTCCAGC AACTGTCTGA CAAGTGTAAA 
TTACAGCCTC CAGGAGCTAC CGGACTTCAA AATCTGAAGA GAGTGCTCGG TTACTGCGAA 
GATACCTATA CCCGCTACAA ACTGGCCTTG GAAAACCAGT TCTATGATGT GACCGATGTT 
TTAATGAGAG ACTCACAAAC CCGCTGCTGC CTGACTGACA TGCTGTCTCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( XM_012958758.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814212.1 Xenopus tropicalis spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X2, mRNA.

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CloneID	OXa25974
Clone ID Related Accession (Same CDS sequence)	XM_012958758.1
Accession Version	XM_012958758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGACTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGATATGG GTTTCTCACA CCCAAGAAAT 
CTGTGGTGTC ATCTTACTTT ATGGCATGCA TGGCAGAGGA TTTCTTCATG GATAGAATCT 
TTAGAAAAAC ATGGAAAGAC AAGTAACAAT GATCTCTCAA CATGGCCTCT TCTGACTCCA 
GAAATAGTGG AGTCATGCAC CCTCTGTTGT GACTATACAA AGCAGAAGAT CCTGGACAAG 
TTAGCAAGAA TGGAAATATT TGTCCCTTCT GAGCAGCAGA ATTTTGAGGC CCTTGTGGAA 
CGTATGGCAG TTAGTAGGAG CATGATGCCG TTTCCTGTCT CCAACCCTCA GTGCAGCACC 
TCTGAGAATC AAGATCGTCA CACCCACTTC GTTTTGCTGT GTGCTGAGCA GAGTCTTCAC 
TACCTATTGT ACACATATCT GGATTATCAC AGGTTGAATT CTCAGCTTTG CTCTGCTTTG 
GGCAATACAA CCTTGCATGA AACCTTGCCT TGGTTTGCAT TTTTGGTACA GATACGAGGT 
GTCAAAGACA ACTCTGAAGA TCCAAGCCGC ATGTTTAATG CTAGCCTCTC CAATGCCCAT 
ATGGTGATGC CAGGGTACCA GCCTAGTATG AGCAGCATGC TGCTGGAGGG ACACACATTG 
CTCGCCCTTG CAACCAATAT GTATGCACCT GGTGGCATTG ACAGGGTGTT GGAGCAGCAT 
GGAGAGTCAG ATTCTGGTCA TAGAAATGTA GACCCTCAGC TTCTTAAAAT GGCTTTAGCT 
CCATATCCGA AGCTCAAGGC AGCTCTCTTC AGTACACACC CAGCTACTGT CAGTTCTCCC 
TCTGACATTT CACTTTACCA TCTCCTACAG GCTCTCTCCC CATTTCAGCC TACACACTTC 
TTCACATGGC AGTCGGTAAA TACCCTTGCT GCAAGTGACA ACAGTGCAGT GCTCCCACAT 
TGCTCAAGTC CAGCCTTGGT CAGCAAGTTC TCTGTGGGTG AGCAACTAGA TGTCTTCTTC 
TTCCTGCGGA GCGGGCGACC ATCAGTCGCA TTCGCTACCT TTCTGGTGTT TCAGCTACTA 
AGAAGCAAAA CCCCCCAACA GCTAATCAAG CAAGTTGCTG AGGATGTTTA TTCCCTGGCA 
CTGTCTTCAT TTCATCTCTC CTCTGTGCTT GCTTCATGCT GCTGCTTCTT TGAGTTACTG 
GGTCTCAACA GCCACAAGCT TCGAGTGGAT GTGAACGTTG CACACATGAT ACTAAGTCAC 
TCAGCTTCTC TGCATGAGGA TCCAGGGGTA CACAAATCCC AGGCACAAGC TTTAGCTCAC 
AGACTTTGCA GACTGGTGGA GAATGAGAGA GAAGCAGCCA TGGATATTTT ACATAGCCTG 
GAGGAAGCTG TATTTTCTGA AGAGTGTCAA AACAGCTCAT CTCTACCCAC TGGCTCCTGT 
TTAACTATTC AGCAGTTCTG CCTCCTCCAT TCTATTCCCA TGAGCACTAG GTATTTGAGG 
AGCTGCGCAC AGCAACAAGA CTGGCTGCAG TTACTTGTGA ACACACACAC TCAGGAACAG 
GTCCTATGTG TAGCTGAAAA GTTGAGCCCG GCACTTAATA ATCACATAAT GCTATCATTG 
CAAGATGCAA ATTCAGATGC ACAAATCTCT GAATATGACT TGGAAAGATC TGAAAATGAT 
ACAGTGACTG CCAGTTTGTA CAATATATTG CTTGGGTGCC AGAAGACTGG GCAGCCAGGC 
CGCACACTTC TAAAGGAATG TATTAAGCAC AGAGCTCCAT TGCTGAGTGT GCTTGCTGCA 
TATATGCAGG ACACCAACCA AATTGCTTGC CTGTGTGTGT GGCTTGTAAC CTCTGTAGAT 
CCGGCCAATT GTGCAGAACT CACAAATATG CTTTCGGTTG CTTCAGATCA TGAGTGGGAC 
TTGCAAGATC TGACACGGAT TTGGGAAATC TTGCTGGAGA GAAAAGATAG CCAGACTTTG 
TACAAAGCAT TCAGCATCTT TTTAGAGGAC TGTCCCCTTC TTCTCTTACT TGAACTGTAT 
GAGCTTTGCC TGCAACATAA ATACTATCTG GAGGCTGGAC AGAAAATAGA TGATTTCCAA 
AGATGGATGA TGAACGATGA CACTGAAGAT ACAGAGTTAT CGCTTCTCAT TCCACTGCCC 
TGGTTGCTAC AAAGAGCTTC TCAACTTCTG CAGCTCCTTC TCTTGCAGAC CCAGACTCCA 
TATGAATTGC GGAAGGTTTT GCAGCTTCTG AGTGACAAAG CTAAACCACA GTTATTTGGC 
GGAATAGATG TTCATAAGCT GAGTGATCTA ACCTCTATTC TTCAAGACCA CCCTATTGCC 
ATTAGCAAAG AATTAATATG CCACTATAGT CCTGAAGCCC TGCAGGTTGA GTGTCAAAGG 
CTTATACAGA TGCTGTTGGA CAAAAGGATT TTTTCTCTTG CACAAAGAGT AGCAGAACTA 
GCAGAATTGC CCACTGACAG TATTGTCATA GAAGAGGTGT TGCAGGACCA ATGTCTCTTA 
AAAGAGGTTG GGCAGTGGGA GAATTCTCAA AGCCGTGTAC AGTACTGGAG AAAATGCCAC 
CAGGTGTTTA GCGCCACGGA GCTCTCCCCA GCTGCAGCGT CTGACTTCTT CTGCTCTCAG 
ACTCTTTCTC TTGTAGCGCC TGAATGCTTG CTTAGGGATC AGACAGAGTT TTTGGCAGAG 
CAGGAGATGC TGTTTACCCT TGCTGGGCAC TGGAGGTCCC TAAGTAACAC TGCTTCTATA 
GAAGTCCTGG AGGAAATTGA GCAGAAGATT TGGAAATGTC GCCTGGCAAG AGAAGTATTG 
AGTAAACATG GAGCTGCTAG TCAACTCTGT ACTCTGCCTT CATTTGCTAG CTTGACCCTG 
GAGTTTTCAT TCTCATCTTT GCCTGCACTA AACTCTCCTT CGCTTCTTCA TATCTCTAAT 
TTGCCACCGC TTGGTACTCC TAGCAGTTTG TTGGACAGAG AACAGACCAA AGCATTGAGC 
TCTTTGGTTG ACTGCCTGTT GGATGAGAGC CGTGTGCACG AGGCCAGTCG GGTATGCAGG 
TACTTTCTGC TACCTCATTA TGATCTTTGG CTACTGCTGA ACTGCAGGGC ACTAGCAACA 
GGAGAGACCG TAAGGGACCA ACTGCATCCG GAAATTCAAG CTATCCTCGG TGAAGGCAGC 
GAACGCCGGG AAAGCATATG GCACCAGAGG AAAAGACTGC ACAGCTCCTC CTCTCTGGAC 
ACCGCCATGC CCCCTGTACC CAGTGATCCT GTGCTCTTAG ATCTGGAAAT TCTGAAGGAC 
AGGTGCTCCC ATGGCAAAAC CTACTGCCAA CAGCTGCTGT GTCTTTACGA GTTAAGCCAG 
GACCTGGGAT GTTCCTTCTC AGATATCTCC TCTCGAGATC CCGGCGAGAT TCTACGCTCT 
CTGCTTTCTT CTCAGCGTCC CGAGCTCACA GACAGAGCAC AAGCAGTCAT CAACTTCCAC 
GGACTGTCGC CGCAAACAGT GGCACAGATT GTGGCAGAAG AGGGTGTGAG AGTCTGGCGG 
GCAGGGCCCG TGGAAGTTTA TAATGCCTCT GAAATAAGGC AGCGCTTTTT GCAGCTTGTA 
AAACTTTGCC AGGACCCAAC TCTGGTTGGA TTAACGCTAC TTGACTATCT AGAAAATGTG 
CCACTGACTG AGCAACACTG CATTATTGAA CTCCTGATTT CTGCCCACGA TTGCTTCAGT 
TTAACGTGCC ATCTTGAAGG AATTCGACGG GTGCTTCAGG CATGTCGACA CCTAACCGAG 
AGTCACTTGG CGGCTAACCA AGAATACAGC CTTATGGTTC GCCTACTCTC TGGGATTGGT 
CGTTATAATG AGATGGTTTA TGTGTTTGAC ATTCTGCACA AGGAACAGCA CTTTGAGGTT 
CTGCTGAGGA AACAGCTAGA TACAAAGGGG GGACTGCAGA CTGCTCTTTT GGAATACATT 
AAGCGCTGCC ATCCAGGAGA CAGCGAGAAG CACAACATGA CTGCGCTCTG CTTCAGTTTG 
CACCGTGACA TTGGCCACAA CCATGAGCAG GCAGCTTTGA TCCAGCTCAA ACTCATCCAG 
TCTAGGGCAT GGGAATACTG GATGTCTGAA TTGGTGGAGT TGAGATCTGC ACTAATGAAA 
GCACTTACCC TCCTAATAGA TGCAGCTGAG AGCTACTCAA AGGATTCCTG CGTCCGTCAG 
TCTCTGCGCT GTGCTCGCCT TACCCGTCTG TTGACTCTGC AGCTTCATCT GCTAAACAAC 
AGTCATCAAA CTAAATTAAT CAACCTGGAT AGAGAGAACT TGATGGAGCC AATACTGGAA 
CTGCCACGTT TCTACCAGGC AGTCATTGTT ACAGAGGCCT ATGATATACA GCCAGACTGG 
GCAGAGGTTT TATATCACAA GGTGATTCTT GCTGGAGACT TTCAGTATTT AAATGAATTC 
AAGCAGCGAG AACTGCTACG GAGTGGAGTG TTCCAGCAAC TGTCTGACAA GTGTAAATTA 
CAGCCTCCAG GAGCTACCGG ACTTCAAAAT CTGAAGAGAG TGCTCGGTTA CTGCGAAGAT 
ACCTATACCC GCTACAAACT GGCCTTGGAA AACCAGTTCT ATGATGTGAC CGATGTTTTA 
ATGAGAGACT CACAAACCCG CTGCTGCCTG ACTGACATGC TGTCTCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814212.1
CDS	215..7285
Translation	MGRRILQSCKMNLNILLVPGLTLPEGTNPPVRVSLTRQKHPTLCSLCPQGELHLTALPYEGFHTTHTQLEGLFSHFLWENADGNEEPSDNIPRLLTLSGSNEVAVHGLHVADGHLNVTTIFLCDEDTLRKLCADNHVCVSSVICVRLLSLQGDDVLLLLSSSVLLHLHFSSEDNSLKLLSCLCLDLGFEATERISDVCLKSGLLFMLDTYGFVYVYDTVDGSPIFTMELPLRPENAALDPLNSICVSDDLGLFVVTTAASWALSVQLCEDAREISDHAVSAWHSDHVPDLHISGADEERLSSCHESVCTPRCTLRSDRSWDATLSSLCNEIMNSSDTFVCSKNPYLWPCNLPLKVPLSGIPVGEGWVRIVSEGLDDPLSLHIGSVTSFSAVLCLFGMDGLLNMIQWDMQSKEISSYPLGKAHFVHCSQEDACLVMSDRGLSLVVFSASQEEFLTRLMIHGSASTADTLCLLNKWGRCSVPIHTLEAGLDNRQLDTVDFFLKSKVSILNPLISEEPPDGIQAERYLQNVQDLLPALDLLVSYIRETDQETQSKHFSEQLLQLTLGFLNGQLHLLCALLSESSPALQQAVEILMGYITKLRRFMKKFMEQPQPVDVPGIEGQLHSWKNLSLEQIISDAILTNRIPEAQVFLRAEGHQACSLTWLKQEGLNLVYKCLLETRLQETRQLLRNMGFSVMNELKRICLHTSDRGVRTLLVDLLQREGCFSDQEQEDIQTVIHLEEVYCCPQKRTKESHKLLPHRLVQRLFTGSQEPEVLQAALTPSKNKSHMVLLYWAQGWDLETKEAILLPLRNSQDMGFSHPRNLWCHLTLWHAWQRISSWIESLEKHGKTSNNDLSTWPLLTPEIVESCTLCCDYTKQKILDKLARMEIFVPSEQQNFEALVERMAVSRSMMPFPVSNPQCSTSENQDRHTHFVLLCAEQSLHYLLYTYLDYHRLNSQLCSALGNTTLHETLPWFAFLVQIRGVKDNSEDPSRMFNASLSNAHMVMPGYQPSMSSMLLEGHTLLALATNMYAPGGIDRVLEQHGESDSGHRNVDPQLLKMALAPYPKLKAALFSTHPATVSSPSDISLYHLLQALSPFQPTHFFTWQSVNTLAASDNSAVLPHCSSPALVSKFSVGEQLDVFFFLRSGRPSVAFATFLVFQLLRSKTPQQLIKQVAEDVYSLALSSFHLSSVLASCCCFFELLGLNSHKLRVDVNVAHMILSHSASLHEDPGVHKSQAQALAHRLCRLVENEREAAMDILHSLEEAVFSEECQNSSSLPTGSCLTIQQFCLLHSIPMSTRYLRSCAQQQDWLQLLVNTHTQEQVLCVAEKLSPALNNHIMLSLQDANSDAQISEYDLERSENDTVTASLYNILLGCQKTGQPGRTLLKECIKHRAPLLSVLAAYMQDTNQIACLCVWLVTSVDPANCAELTNMLSVASDHEWDLQDLTRIWEILLERKDSQTLYKAFSIFLEDCPLLLLLELYELCLQHKYYLEAGQKIDDFQRWMMNDDTEDTELSLLIPLPWLLQRASQLLQLLLLQTQTPYELRKVLQLLSDKAKPQLFGGIDVHKLSDLTSILQDHPIAISKELICHYSPEALQVECQRLIQMLLDKRIFSLAQRVAELAELPTDSIVIEEVLQDQCLLKEVGQWENSQSRVQYWRKCHQVFSATELSPAAASDFFCSQTLSLVAPECLLRDQTEFLAEQEMLFTLAGHWRSLSNTASIEVLEEIEQKIWKCRLAREVLSKHGAASQLCTLPSFASLTLEFSFSSLPALNSPSLLHISNLPPLGTPSSLLDREQTKALSSLVDCLLDESRVHEASRVCRYFLLPHYDLWLLLNCRALATGETVRDQLHPEIQAILGEGSERRESIWHQRKRLHSSSSLDTAMPPVPSDPVLLDLEILKDRCSHGKTYCQQLLCLYELSQDLGCSFSDISSRDPGEILRSLLSSQRPELTDRAQAVINFHGLSPQTVAQIVAEEGVRVWRAGPVEVYNASEIRQRFLQLVKLCQDPTLVGLTLLDYLENVPLTEQHCIIELLISAHDCFSLTCHLEGIRRVLQACRHLTESHLAANQEYSLMVRLLSGIGRYNEMVYVFDILHKEQHFEVLLRKQLDTKGGLQTALLEYIKRCHPGDSEKHNMTALCFSLHRDIGHNHEQAALIQLKLIQSRAWEYWMSELVELRSALMKALTLLIDAAESYSKDSCVRQSLRCARLTRLLTLQLHLLNNSHQTKLINLDRENLMEPILELPRFYQAVIVTEAYDIQPDWAEVLYHKVILAGDFQYLNEFKQRELLRSGVFQQLSDKCKLQPPGATGLQNLKRVLGYCEDTYTRYKLALENQFYDVTDVLMRDSQTRCCLTDMLSR

Target ORF information:

RefSeq Version	XM_012958758.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958758.1

ORF Insert Sequence:

ATGGGAAGGA GAATATTACA AAGCTGTAAG ATGAATCTGA ACATCCTTCT GGTGCCAGGA 
CTCACACTGC CTGAAGGGAC CAATCCACCA GTGAGAGTAT CCCTGACAAG GCAGAAGCAC 
CCTACTCTCT GCAGTCTTTG CCCTCAAGGG GAACTTCACC TGACAGCGCT CCCATATGAA 
GGCTTCCACA CAACCCATAC TCAGCTGGAA GGACTTTTCT CTCATTTCCT GTGGGAAAAT 
GCTGATGGGA ATGAAGAACC ATCTGACAAC ATCCCCCGTC TTCTTACTCT GAGTGGGAGC 
AATGAAGTTG CTGTTCATGG CCTGCATGTT GCAGACGGGC ACTTGAATGT GACCACCATC 
TTTCTTTGCG ATGAGGACAC TTTGAGGAAA CTTTGTGCAG ATAACCATGT TTGTGTGTCC 
TCAGTCATCT GTGTTCGGCT ATTGTCCCTA CAAGGAGATG ATGTTCTGCT GTTGCTGAGT 
AGCAGTGTCT TGTTGCATCT TCACTTTTCT TCAGAAGATA ACAGTCTAAA ACTTTTATCC 
TGCTTATGCT TGGATTTGGG TTTCGAAGCA ACAGAGCGCA TTTCAGATGT ATGTTTAAAA 
AGTGGGCTGT TGTTTATGCT GGATACTTAT GGTTTTGTAT ATGTTTATGA TACTGTAGAT 
GGCTCACCCA TCTTCACCAT GGAACTTCCA CTAAGACCAG AGAATGCTGC TTTAGATCCA 
CTCAACAGTA TTTGTGTGTC TGATGACCTA GGCTTATTTG TAGTCACCAC TGCTGCTTCC 
TGGGCTCTCT CTGTGCAACT CTGTGAAGAT GCCAGAGAGA TCTCAGATCA CGCAGTATCT 
GCCTGGCACA GTGATCATGT CCCAGATCTA CATATATCAG GTGCAGATGA AGAGAGACTG 
AGTAGTTGTC ATGAAAGTGT TTGTACCCCT AGATGTACCT TACGCTCAGA CAGATCTTGG 
GATGCCACAC TGTCTTCACT ATGCAATGAG ATCATGAACT CTTCAGACAC GTTTGTGTGC 
TCCAAGAACC CTTATCTATG GCCTTGTAAC CTTCCTTTAA AAGTACCTCT CAGTGGGATC 
CCTGTGGGGG AAGGCTGGGT GAGGATTGTT TCTGAAGGGT TGGATGATCC TTTGAGTCTC 
CACATCGGGT CTGTCACTTC CTTCAGTGCT GTACTGTGTC TTTTTGGGAT GGATGGCTTA 
CTTAACATGA TACAATGGGA CATGCAATCA AAGGAAATCT CTTCCTATCC CTTGGGAAAG 
GCTCACTTTG TTCACTGTTC TCAGGAAGAT GCTTGCCTGG TGATGTCAGA TCGAGGGCTA 
TCTCTAGTGG TGTTTTCTGC TTCACAAGAG GAATTCCTGA CCAGATTAAT GATTCATGGC 
AGTGCAAGTA CAGCAGATAC CTTATGTCTT CTAAATAAGT GGGGCCGTTG CTCCGTTCCC 
ATTCACACAC TAGAGGCTGG TCTAGACAAC AGACAGTTAG ACACTGTTGA CTTTTTCCTG 
AAGAGCAAAG TAAGCATCCT AAACCCTTTG ATCTCTGAGG AGCCTCCCGA TGGCATCCAG 
GCGGAACGCT ATCTGCAAAA TGTGCAGGAT CTTCTGCCAG CTCTTGATCT TCTTGTCTCC 
TACATCAGAG AAACAGACCA GGAGACACAA AGCAAGCACT TCTCTGAGCA GTTGTTGCAG 
CTCACTCTTG GCTTCCTGAA TGGACAACTT CATCTGCTCT GTGCGCTGCT ATCTGAGTCA 
AGTCCTGCAT TGCAGCAAGC TGTTGAGATT CTTATGGGTT ATATAACCAA GCTACGCCGC 
TTCATGAAGA AATTTATGGA ACAGCCACAA CCTGTGGACG TCCCAGGTAT TGAGGGACAG 
TTACACTCTT GGAAGAATCT GTCATTGGAG CAAATAATCT CTGATGCCAT TTTGACAAAC 
CGCATTCCTG AGGCCCAGGT ATTCCTGCGT GCTGAGGGTC ATCAAGCTTG CAGCCTGACA 
TGGTTGAAAC AAGAAGGCCT TAATCTGGTG TACAAATGCC TGCTGGAGAC ACGCCTGCAA 
GAGACTCGTC AGCTTCTCCG AAATATGGGC TTTTCTGTCA TGAATGAGCT TAAGCGCATT 
TGTTTGCACA CATCAGATCG GGGCGTCAGG ACATTGCTGG TAGATCTCCT GCAGAGAGAG 
GGGTGTTTTT CAGACCAAGA GCAGGAAGAT ATTCAAACTG TGATCCATTT GGAAGAAGTT 
TACTGTTGTC CACAAAAAAG GACTAAAGAG AGTCATAAAC TTCTGCCTCA CAGGCTTGTA 
CAGAGGCTTT TCACTGGATC TCAGGAACCA GAAGTCCTAC AAGCAGCTTT GACACCTTCT 
AAGAACAAGT CACACATGGT CCTCCTCTAC TGGGCTCAGG GCTGGGATTT GGAAACAAAG 
GAAGCTATTT TACTGCCCCT GAGGAACAGT CAAGATATGG GTTTCTCACA CCCAAGAAAT 
CTGTGGTGTC ATCTTACTTT ATGGCATGCA TGGCAGAGGA TTTCTTCATG GATAGAATCT 
TTAGAAAAAC ATGGAAAGAC AAGTAACAAT GATCTCTCAA CATGGCCTCT TCTGACTCCA 
GAAATAGTGG AGTCATGCAC CCTCTGTTGT GACTATACAA AGCAGAAGAT CCTGGACAAG 
TTAGCAAGAA TGGAAATATT TGTCCCTTCT GAGCAGCAGA ATTTTGAGGC CCTTGTGGAA 
CGTATGGCAG TTAGTAGGAG CATGATGCCG TTTCCTGTCT CCAACCCTCA GTGCAGCACC 
TCTGAGAATC AAGATCGTCA CACCCACTTC GTTTTGCTGT GTGCTGAGCA GAGTCTTCAC 
TACCTATTGT ACACATATCT GGATTATCAC AGGTTGAATT CTCAGCTTTG CTCTGCTTTG 
GGCAATACAA CCTTGCATGA AACCTTGCCT TGGTTTGCAT TTTTGGTACA GATACGAGGT 
GTCAAAGACA ACTCTGAAGA TCCAAGCCGC ATGTTTAATG CTAGCCTCTC CAATGCCCAT 
ATGGTGATGC CAGGGTACCA GCCTAGTATG AGCAGCATGC TGCTGGAGGG ACACACATTG 
CTCGCCCTTG CAACCAATAT GTATGCACCT GGTGGCATTG ACAGGGTGTT GGAGCAGCAT 
GGAGAGTCAG ATTCTGGTCA TAGAAATGTA GACCCTCAGC TTCTTAAAAT GGCTTTAGCT 
CCATATCCGA AGCTCAAGGC AGCTCTCTTC AGTACACACC CAGCTACTGT CAGTTCTCCC 
TCTGACATTT CACTTTACCA TCTCCTACAG GCTCTCTCCC CATTTCAGCC TACACACTTC 
TTCACATGGC AGTCGGTAAA TACCCTTGCT GCAAGTGACA ACAGTGCAGT GCTCCCACAT 
TGCTCAAGTC CAGCCTTGGT CAGCAAGTTC TCTGTGGGTG AGCAACTAGA TGTCTTCTTC 
TTCCTGCGGA GCGGGCGACC ATCAGTCGCA TTCGCTACCT TTCTGGTGTT TCAGCTACTA 
AGAAGCAAAA CCCCCCAACA GCTAATCAAG CAAGTTGCTG AGGATGTTTA TTCCCTGGCA 
CTGTCTTCAT TTCATCTCTC CTCTGTGCTT GCTTCATGCT GCTGCTTCTT TGAGTTACTG 
GGTCTCAACA GCCACAAGCT TCGAGTGGAT GTGAACGTTG CACACATGAT ACTAAGTCAC 
TCAGCTTCTC TGCATGAGGA TCCAGGGGTA CACAAATCCC AGGCACAAGC TTTAGCTCAC 
AGACTTTGCA GACTGGTGGA GAATGAGAGA GAAGCAGCCA TGGATATTTT ACATAGCCTG 
GAGGAAGCTG TATTTTCTGA AGAGTGTCAA AACAGCTCAT CTCTACCCAC TGGCTCCTGT 
TTAACTATTC AGCAGTTCTG CCTCCTCCAT TCTATTCCCA TGAGCACTAG GTATTTGAGG 
AGCTGCGCAC AGCAACAAGA CTGGCTGCAG TTACTTGTGA ACACACACAC TCAGGAACAG 
GTCCTATGTG TAGCTGAAAA GTTGAGCCCG GCACTTAATA ATCACATAAT GCTATCATTG 
CAAGATGCAA ATTCAGATGC ACAAATCTCT GAATATGACT TGGAAAGATC TGAAAATGAT 
ACAGTGACTG CCAGTTTGTA CAATATATTG CTTGGGTGCC AGAAGACTGG GCAGCCAGGC 
CGCACACTTC TAAAGGAATG TATTAAGCAC AGAGCTCCAT TGCTGAGTGT GCTTGCTGCA 
TATATGCAGG ACACCAACCA AATTGCTTGC CTGTGTGTGT GGCTTGTAAC CTCTGTAGAT 
CCGGCCAATT GTGCAGAACT CACAAATATG CTTTCGGTTG CTTCAGATCA TGAGTGGGAC 
TTGCAAGATC TGACACGGAT TTGGGAAATC TTGCTGGAGA GAAAAGATAG CCAGACTTTG 
TACAAAGCAT TCAGCATCTT TTTAGAGGAC TGTCCCCTTC TTCTCTTACT TGAACTGTAT 
GAGCTTTGCC TGCAACATAA ATACTATCTG GAGGCTGGAC AGAAAATAGA TGATTTCCAA 
AGATGGATGA TGAACGATGA CACTGAAGAT ACAGAGTTAT CGCTTCTCAT TCCACTGCCC 
TGGTTGCTAC AAAGAGCTTC TCAACTTCTG CAGCTCCTTC TCTTGCAGAC CCAGACTCCA 
TATGAATTGC GGAAGGTTTT GCAGCTTCTG AGTGACAAAG CTAAACCACA GTTATTTGGC 
GGAATAGATG TTCATAAGCT GAGTGATCTA ACCTCTATTC TTCAAGACCA CCCTATTGCC 
ATTAGCAAAG AATTAATATG CCACTATAGT CCTGAAGCCC TGCAGGTTGA GTGTCAAAGG 
CTTATACAGA TGCTGTTGGA CAAAAGGATT TTTTCTCTTG CACAAAGAGT AGCAGAACTA 
GCAGAATTGC CCACTGACAG TATTGTCATA GAAGAGGTGT TGCAGGACCA ATGTCTCTTA 
AAAGAGGTTG GGCAGTGGGA GAATTCTCAA AGCCGTGTAC AGTACTGGAG AAAATGCCAC 
CAGGTGTTTA GCGCCACGGA GCTCTCCCCA GCTGCAGCGT CTGACTTCTT CTGCTCTCAG 
ACTCTTTCTC TTGTAGCGCC TGAATGCTTG CTTAGGGATC AGACAGAGTT TTTGGCAGAG 
CAGGAGATGC TGTTTACCCT TGCTGGGCAC TGGAGGTCCC TAAGTAACAC TGCTTCTATA 
GAAGTCCTGG AGGAAATTGA GCAGAAGATT TGGAAATGTC GCCTGGCAAG AGAAGTATTG 
AGTAAACATG GAGCTGCTAG TCAACTCTGT ACTCTGCCTT CATTTGCTAG CTTGACCCTG 
GAGTTTTCAT TCTCATCTTT GCCTGCACTA AACTCTCCTT CGCTTCTTCA TATCTCTAAT 
TTGCCACCGC TTGGTACTCC TAGCAGTTTG TTGGACAGAG AACAGACCAA AGCATTGAGC 
TCTTTGGTTG ACTGCCTGTT GGATGAGAGC CGTGTGCACG AGGCCAGTCG GGTATGCAGG 
TACTTTCTGC TACCTCATTA TGATCTTTGG CTACTGCTGA ACTGCAGGGC ACTAGCAACA 
GGAGAGACCG TAAGGGACCA ACTGCATCCG GAAATTCAAG CTATCCTCGG TGAAGGCAGC 
GAACGCCGGG AAAGCATATG GCACCAGAGG AAAAGACTGC ACAGCTCCTC CTCTCTGGAC 
ACCGCCATGC CCCCTGTACC CAGTGATCCT GTGCTCTTAG ATCTGGAAAT TCTGAAGGAC 
AGGTGCTCCC ATGGCAAAAC CTACTGCCAA CAGCTGCTGT GTCTTTACGA GTTAAGCCAG 
GACCTGGGAT GTTCCTTCTC AGATATCTCC TCTCGAGATC CCGGCGAGAT TCTACGCTCT 
CTGCTTTCTT CTCAGCGTCC CGAGCTCACA GACAGAGCAC AAGCAGTCAT CAACTTCCAC 
GGACTGTCGC CGCAAACAGT GGCACAGATT GTGGCAGAAG AGGGTGTGAG AGTCTGGCGG 
GCAGGGCCCG TGGAAGTTTA TAATGCCTCT GAAATAAGGC AGCGCTTTTT GCAGCTTGTA 
AAACTTTGCC AGGACCCAAC TCTGGTTGGA TTAACGCTAC TTGACTATCT AGAAAATGTG 
CCACTGACTG AGCAACACTG CATTATTGAA CTCCTGATTT CTGCCCACGA TTGCTTCAGT 
TTAACGTGCC ATCTTGAAGG AATTCGACGG GTGCTTCAGG CATGTCGACA CCTAACCGAG 
AGTCACTTGG CGGCTAACCA AGAATACAGC CTTATGGTTC GCCTACTCTC TGGGATTGGT 
CGTTATAATG AGATGGTTTA TGTGTTTGAC ATTCTGCACA AGGAACAGCA CTTTGAGGTT 
CTGCTGAGGA AACAGCTAGA TACAAAGGGG GGACTGCAGA CTGCTCTTTT GGAATACATT 
AAGCGCTGCC ATCCAGGAGA CAGCGAGAAG CACAACATGA CTGCGCTCTG CTTCAGTTTG 
CACCGTGACA TTGGCCACAA CCATGAGCAG GCAGCTTTGA TCCAGCTCAA ACTCATCCAG 
TCTAGGGCAT GGGAATACTG GATGTCTGAA TTGGTGGAGT TGAGATCTGC ACTAATGAAA 
GCACTTACCC TCCTAATAGA TGCAGCTGAG AGCTACTCAA AGGATTCCTG CGTCCGTCAG 
TCTCTGCGCT GTGCTCGCCT TACCCGTCTG TTGACTCTGC AGCTTCATCT GCTAAACAAC 
AGTCATCAAA CTAAATTAAT CAACCTGGAT AGAGAGAACT TGATGGAGCC AATACTGGAA 
CTGCCACGTT TCTACCAGGC AGTCATTGTT ACAGAGGCCT ATGATATACA GCCAGACTGG 
GCAGAGGTTT TATATCACAA GGTGATTCTT GCTGGAGACT TTCAGTATTT AAATGAATTC 
AAGCAGCGAG AACTGCTACG GAGTGGAGTG TTCCAGCAAC TGTCTGACAA GTGTAAATTA 
CAGCCTCCAG GAGCTACCGG ACTTCAAAAT CTGAAGAGAG TGCTCGGTTA CTGCGAAGAT 
ACCTATACCC GCTACAAACT GGCCTTGGAA AACCAGTTCT ATGATGTGAC CGATGTTTTA 
ATGAGAGACT CACAAACCCG CTGCTGCCTG ACTGACATGC TGTCTCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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spg11 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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