LOC101732997 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following LOC101732997 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101732997 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa38243 XM_012960202.2
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain-like (LOC101732997), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54924 XM_031899076.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54924 XM_031899075.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54925 XM_031899077.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54926 XM_031899078.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54927 XM_031899079.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54928 XM_031899080.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54929 XM_031899081.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa54930 XM_031899082.1
Latest version!
Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa38243
    Clone ID Related Accession (Same CDS sequence) XM_012960202.2
    Accession Version XM_012960202.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT CACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAATACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CACCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACAGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GACACTCCTA AAAGTCATTA TCTGGCGTCC GGTGTCAGGT CCTATCACAT CACAAATTTG
    TACCCAAACA CAAAGTACAC TGTGAATGTT CAGGCAGTAT ATAATAACTT TGAAGGGCCG
    CCAAGCACTC TAATTCAAAC CACAGACTCA AATCCTGTGC AGCTACTGCC TGTGAAAGAT
    CTGCAGATCA TTGATACTGG AGTCAATATG CTGAAACTGT CTTGGAAGAA AACACCTGGA
    ATAACACATT ATAAGATCTC ATGGACACCA TTTGATGGTG GTGCAAGAAA CTCTAAGATT
    GTCTCTGCTG GTACGTCATT TTTCACAATC CCTGACCTTA AGGAGAGTTC CACTTACAGC
    ATCTATGTAT CCTCGATAAT CGGGAATCGG GAAGGAAGTT CAGTGGTTCT TACTGCAAAA
    ACATTGGATT TGCCAAAAGT AGAATCTTTT GAAGTCAAAG AGACCACAGA GGACAGTGTT
    TTATTAAGCT GGACTGGAGT CACTGGAGCA TCTTCATATA TTTTAACATG GAGGATGGCT
    TCAGACCCAG AATCCTCCGT GGAGCAGTTA GCTCCAACAT ATAGATCTTT CCGGCTCATG
    AATTTGGAGA ATGGAAAGTC ATATATTTTT GCAATAAAGC CTCTTTTTGG AGAGATGGAA
    GGGCCTGAGA GAACAATCAC ACACACAATC GTTGGACCCC CCAAAAAACA GATCTACCCT
    TCCCCTACCC TTCAAATACC TGTTCCAACA CCTCAAAGGA CCCTAACAAC ACAAGTTATC
    AACCACCCTC CCATAGTCAC CACAACTGAA GCTGCAAAAT CCACAAGCAC TGTCTCACAT
    GGTTCTTCCA CAACTACTTC TGAACCCATT TGTGCAAAAC TGAAAGCTGA CATAGTATTT
    CTGGTTGATG AGTCCTCCAG CATTGGCCCA TCCAATTTTA TTAAGGTTAA GGATTTCCTC
    TACAGAATTG TCTCTTACTT TCCCAAAATT GGACCTCAAG GAACACAGAT TGCTATTGTT
    CAGTACAGCG AAGAACCCAG AACAGAATTC CACTTGAATG AGTATAAGGA GAGAAATTCT
    GTTTTGAAAG CAATCAGAGC TCTACAGTAT TATGGAGGCA ACACCAAGAC TGGTCGTGGT
    ATAGGACATG CACTGAAGGA AATGTTTCAG GTTTCCAAAG GGATGAGACC ATCTGCTCCA
    CATATACTTG TGCTAATAAC TGACGGAAGG TCTCAGGATA ATGTAATACA GCCAGCCAAA
    GTAGCTCATG TATTAGGCAT TCGTATGATT GCAGTCGGTG TGGCTGGGGC AGATATGGAT
    GAACTGAAAG GAATGATGAT GAACCGTAAT TTAGAAAACA TCTTCTTTGC CAGTACATTT
    GATGATTTTC CTCTAATTGT ACAAGATCTG ATAGATGCTA TTTGCACTGA AACCGAACAT
    GCAGTTAAAG AAGAACCTAC TAAGTTCAGA GCACCAGATA AAGAAGAGGA GCCAGAAAGA
    CCAAAACCTC CTCCTGGGAA TCGAGAGGGA CCCTGTGGAA ATACCATGTG CCCAACGGGG
    CAAAAGGGAC AAAAGGGAGA AAAGGGTCCA CCGGGAGATT CAGTTTTCAC AGGTTTACAG
    ACTGGTGGCT TTGACCCTTT TGATTTGAAT TCAAAAGGAG AAAAAGGAGA GAGAGGTCTT
    CCTGGACAAG ATGGTGTTCC TGGCCTTCCA GGACGTCCTG GTCGAACTGG GCCCCCAGGG
    CCACCTGGCC TTATGGGACA AAAAGGATTT CAAGGCGATA TTGGCCCCCA AGGATATCCA
    GGTCAGCCAG GTCCCAAAGG AGACAGGGGA GAACCAGGCT ATATACTAGG AGGAGTTGAT
    GGAATGGCTG GTCTAAGTGC AATACCTGGG TCTCCTGGAC CCAAAGGACA GCCTGGGGTT
    CCAGGGCTAC CAGGACCACC TGGACTTCCA GGATTTCCTG GACCTCAGGG ACCTCCAGGA
    ACTTCTATAA AGGGGGAACC TGGTGAAACA GGGAGCCAGG GTCCTCGTGG AAAAAGTGGA
    CCAAAAGGAG ATAAAGGAGA CCAGGGGGAT GCAGGAAGAG CTGGCTTACC TGGCCCAATT
    GGGCTTGATG GCTCTCCAGG AATTCATGGA ATTAAAGGAG ACAAGGGGGA GGCTGGTATT
    GGAATCCCTG GATTACCTGG TTTAAAGGGT ATAACAGGTG AAAAGGGTGT CATTGGACTT
    CCTGGCCCTC AAGGACAGAA GGGTACTCAA GGAATACAAG GTGTAGAAGG TGTCGCAGGT
    TTAAGGGGTA AAAAAGGTCA AGATGGTGAA AAGGGTGAAA AGGGAGAACA TGGTGCTATG
    GGAATGACTG GTCCTCCTGG AATAGCTGGT CTCTCTGGAC CAATGGGGCA AAAGGGGGAT
    CAGGGGTTGC AAGGTTTTCC TGGCGCTCCT GCTATGGGGG TAATTGGGCC TTCTGGTAAA
    AAAGGTGCCA GAGGTGATAT TGGACCAGTT GGGCCAATTG GACCTAAAGG CATTATGGGA
    AGTCAAGGAG ATAAAGGAAT TAAGGGAGAA CCAGGATATG GTATACCTGG GCAGCCTGGA
    TTAAAGGGAG ATCCAGGTGA CAGAGGAAAC ATAGGATTAT CTGGAAAACC AGGTCCCAAA
    GGAGATTTTG GAGACAAAGG ACAGAAGGGT GAGCCTGGAA ACTTTGGTGC CCTAGGACAG
    TCTGGACTAA GAGGTAAAGA TGGTGAACCA GGACTAAATG GGGACAAAGG CGAGAAGGGA
    GACGCAGGTG TCCCCGGAGA ACCAGGGGAA AAGGGACAAA GGGGCTCTGT TGGACTGCCT
    GGCCGGATTG GTGATACTGG AATAAAAGGA GATATGGGGC CACCTGGAAG AGATGGCATA
    GATAGTAAAA AAGGTGATAA AGGAGAAGCC GGGCAGCCAG GAACACCTGG GGCAATGGTG
    ACTCAAATAG ACAAAAGTGT GCTTGTCAAA GGAGAAAAGG GAGAACCCGG AGAGGTGGGA
    GATCCAGGAG AATCGGGTTT TAAAGGAGAA AAAGGAGACA CGGGGCTACC AGGGCCCAGA
    GGACCTGAAG GCCGTCCAGG ACCACCTGGT GCTTCACCGT TGGAGTCCGC TTCTGAGAAA
    GGAATGAAAG GGGAAAAGGG AAGCCCAGGT TCTTCTGGAA AAAATGGCCT TGATGGAAAG
    CCTGGGAATC CTGGGTTACT TGGACAAAAG GGAGAAGCAG GCCAAAAAGG AGAACCAGGA
    AATGTAGGCC TAACTGTAAG TGAAAAGGGA AATAAAGGAG AAATAGGAAA CCAAGGCCCA
    CCAGGACCTC AGGGACCTCC TTGCACAGAT TTAGAAATGA AGGATTTCGA AAGGCTATTT
    GAATCTTATG GCATTAAGCT GTCTCTACTG AAGGAACTGA CTGATCATAT TATACAAAAT
    GGATTAGCTG ATGTAATTCA GCAACTAACA AATTCCAAGA AAGACCGAAC TTCTAAGAAG
    AAGCAAGTTT ATAAACAGTC ACCAGAATTC ACTGATGCCT TAAAACAAGA TCATATTAAT
    GAGGCAGAAA AAAACCATGA GGAAGAAGAA GATCCAAGCT TGCAGGCTGA GATAATACCT
    ACAGTGGGGG TGACTGAGTC CCATACTCTA ACTCAGGACT TAAAAGAAGG CAATAAAGAA
    CAAAAGAAAA AGAAGAAAAC ACATGTAGAG GCTGTTCCTA TTAACATTTA CACAAATGTG
    ATCACTGAGG AACAGGAGGA AATCAGCCAA GACCAGAGCC ACCATTTAAC AACCAATACT
    ACTTTTTTGC CAACTCAGCA ATCAATAGAT TTGGAAGGAT CTGATGCAAA TGCTGATAGA
    GTTGTCCAAG CACAGATTGA CAAACATCAG AGGAAGCAAG AGAAAGAGGT TAGAAAGGAA
    AGGAAAAAAA ATCGCCTTGA AACTACAGCT ACTGAAACAG AAATGAAGAA CCTTGTTTTG
    GAGGAAATTA CAGCAGTGAC TGCTTATAAA GAAGAAAATT CACAATCTGC CACCACCCTG
    CCTGAAATAA TTAATGCAAA GAGGTTAACA TCTGACTTGA TAGAGGAGAC AGACACAATC
    GTTCAGCCCA CATCTGATAC TACTATACTA GTCGAAAAGC ATCAGGAAAA GGCAGTGAAA
    GAGAGCAAGA AGGACCAGAA AAAGAAAATG CGTACTGATC ATAAAGTCAA AGAACTCAAA
    ATACAGGGAA CTGCACAACA AGAGTTAAAT CAGATACTTT CTGAGAGAAC AAGCAGGGAT
    CCCCACAGTG TTGATTCAAA TGCAGCAGCA AGCACTGAGC ATAGTTTGGA GAATCAGCAG
    GTCACCGGAA ATATATCCTT CAGGGTCAGC CTAAAAGACA ATGACTTGAC AATGATTGAT
    GAGACGTCTT GGAATGTCAC AGTAGTTGAA ACTGATAAAG CAGATCAAAC TGCATCACAG
    GAGGATGAAG AATACTTGGA GGTGCCAGAA ACTGAGACCA TCACCAGTTC CATTAACTCA
    TCAGAACATA TGGGACACAA TCGAATCAGG AGGATGGCAA AAGATTTAGA AAGGATATTC
    GCCCCAGAGA AACTAAGAAG ACAAAGGAGA ACAAAGGAAG GAAAGGGGCG TTCAGGATCT
    CAAAGAGACA AAGGACAAAA GGGTGAACCA GGGGAGGCTG GTGAGGCTGG GATTCCTGGT
    CTGAAGGGTG AAAAGGGAGA TATTGGAGAA CAGGGACAAA AGGGGGAACC TGGTGTTGGA
    CACCGTGGGC CAATTGGACA GGCTGGACCA CCAGGTGTTA AGGGTGAGCC AGGAGAGACG
    GGACCCCCAG GAGCTCAAGG AATTCAAGGT ATCCAAGGCA ACCCAGGCAT CTCAGGATTC
    CAAGGTGAAA GAGGAGAAAC AGGATTTTCA GGGCCTCCTG GACCACCTGG AGAGAGAGGC
    AAGAAGGGAA AAAATGGAAA TGCTGGTCAA CCTGGGCCCC ATGGATCAAC AGGAAAACAG
    GGGAAACCAG GACTTCAAGG TAGTAAGGGA GAAAAGGGTG AAGCTGTGGT TGGTGAACCT
    GGTCCTCGTG GGCCTCGCGG GCTTCCTGGC TTTAGGGGAG AAAATGGGAT CCCAGGCTTA
    CCTGGACCTG CGGGAATTGT AGGTTTAAAG GGATTCCCAG GAATAAAGGG TGAACGAGGT
    GATCGGGGGA TTTTTGGGAT TAAGGGAGAA AAGGGTGAAT CCACAACTCT CTTTGGCTCT
    CAAGGGTATA AAGGAAACAA AGGAGATCCG GGTGACAGAG GCTACCCAGG CTTTGATGGA
    GACAAAGGCG AGAAAGGAGA AGATGGACCT GCTGGGGAAA AAGGTGTGAA AGGTGAAGCT
    GGTTCCAAAG GATCCATGGG ACTATTTGGA GCTCGGGGTC CTGTTGGACA GAAGGGTGAG
    CCAGGGGAGC ATGGACTTCC AGGAAGAGCA GGAACAGCAG GACTTGATGG AAAAAATGGA
    CTCAAAGGAA CAAAAGGTGA CCGGGGTCTA CAAGGACAAA GGGGAAACCC GGGAGAGAAA
    GGTGATTCTG GATTGTCAGG AGATCCTGGA AAGAAAGGAG ATAAGGGTCT GCGGGGAATT
    CCAGGAAAAA TGGGAGAGCC TGGGACAGAA GGTGAAAAAG GTGACCCTGG GGCTCAAGGA
    AATCCCGGTA CAGGATCAGA AGGTCCTTCT GGACCTAAGG GTGAGAAAGG TGATAAAGGT
    GAAGATGGAC CCCCTGGAAT TGCTGGAGAT AAAGGATTTA AGGGTACTGA TGGAGTGCGT
    GGACTGAATG GCTTTAGGGG ACCAATAGGC CATCCAGGAA AAGATGGCCC TCCAGGTCCT
    ACTGGACCTT CAGGACAGAA AGGAGAGCCA GGGCCAAAGG GTGAAAGAGG AAAAAGGGGA
    AAATCCTTGC AGTGTCCAAG AGGAACCCCA GGGGATCCAG GAAACAAAGG AGAAACGGGA
    GTGGAGGGGC CTGAAGGTCT CAGAGGGGAC AAGGGAGATC CAGGGCTCAC AGAGGAAGAA
    GTGAAAGATT TGGTCAGAAA AGAAATGAGC GACAAATGTG CTTGTGCAGG AAAGGATTTT
    CATCTAGTAA TTAAATCATC ACTCCCAGAT GAAGAGTATG AAGATCCTGA CCTAAATGAA
    AGTCCAGTGA CTGCTCCACC ACAGGATATT GTGACTGAAA ATGATGTAGT AGTAGAAGCG
    TATGGAAATG TAACACTTAA TAGCACTTTA ACTCCAGATA CGGAAGTGCA ACAAAACGAC
    ACAGGTAATA GAATAATCAC AAGCATCCAT AAAATGTACA TTGTTACAAT GTCTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815656.1
    CDS594..8813
    Translation

    Target ORF information:

    RefSeq Version XM_012960202.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain-like (LOC101732997), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012960202.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT CACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAATACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CACCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACAGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GACACTCCTA AAAGTCATTA TCTGGCGTCC GGTGTCAGGT CCTATCACAT CACAAATTTG
    TACCCAAACA CAAAGTACAC TGTGAATGTT CAGGCAGTAT ATAATAACTT TGAAGGGCCG
    CCAAGCACTC TAATTCAAAC CACAGACTCA AATCCTGTGC AGCTACTGCC TGTGAAAGAT
    CTGCAGATCA TTGATACTGG AGTCAATATG CTGAAACTGT CTTGGAAGAA AACACCTGGA
    ATAACACATT ATAAGATCTC ATGGACACCA TTTGATGGTG GTGCAAGAAA CTCTAAGATT
    GTCTCTGCTG GTACGTCATT TTTCACAATC CCTGACCTTA AGGAGAGTTC CACTTACAGC
    ATCTATGTAT CCTCGATAAT CGGGAATCGG GAAGGAAGTT CAGTGGTTCT TACTGCAAAA
    ACATTGGATT TGCCAAAAGT AGAATCTTTT GAAGTCAAAG AGACCACAGA GGACAGTGTT
    TTATTAAGCT GGACTGGAGT CACTGGAGCA TCTTCATATA TTTTAACATG GAGGATGGCT
    TCAGACCCAG AATCCTCCGT GGAGCAGTTA GCTCCAACAT ATAGATCTTT CCGGCTCATG
    AATTTGGAGA ATGGAAAGTC ATATATTTTT GCAATAAAGC CTCTTTTTGG AGAGATGGAA
    GGGCCTGAGA GAACAATCAC ACACACAATC GTTGGACCCC CCAAAAAACA GATCTACCCT
    TCCCCTACCC TTCAAATACC TGTTCCAACA CCTCAAAGGA CCCTAACAAC ACAAGTTATC
    AACCACCCTC CCATAGTCAC CACAACTGAA GCTGCAAAAT CCACAAGCAC TGTCTCACAT
    GGTTCTTCCA CAACTACTTC TGAACCCATT TGTGCAAAAC TGAAAGCTGA CATAGTATTT
    CTGGTTGATG AGTCCTCCAG CATTGGCCCA TCCAATTTTA TTAAGGTTAA GGATTTCCTC
    TACAGAATTG TCTCTTACTT TCCCAAAATT GGACCTCAAG GAACACAGAT TGCTATTGTT
    CAGTACAGCG AAGAACCCAG AACAGAATTC CACTTGAATG AGTATAAGGA GAGAAATTCT
    GTTTTGAAAG CAATCAGAGC TCTACAGTAT TATGGAGGCA ACACCAAGAC TGGTCGTGGT
    ATAGGACATG CACTGAAGGA AATGTTTCAG GTTTCCAAAG GGATGAGACC ATCTGCTCCA
    CATATACTTG TGCTAATAAC TGACGGAAGG TCTCAGGATA ATGTAATACA GCCAGCCAAA
    GTAGCTCATG TATTAGGCAT TCGTATGATT GCAGTCGGTG TGGCTGGGGC AGATATGGAT
    GAACTGAAAG GAATGATGAT GAACCGTAAT TTAGAAAACA TCTTCTTTGC CAGTACATTT
    GATGATTTTC CTCTAATTGT ACAAGATCTG ATAGATGCTA TTTGCACTGA AACCGAACAT
    GCAGTTAAAG AAGAACCTAC TAAGTTCAGA GCACCAGATA AAGAAGAGGA GCCAGAAAGA
    CCAAAACCTC CTCCTGGGAA TCGAGAGGGA CCCTGTGGAA ATACCATGTG CCCAACGGGG
    CAAAAGGGAC AAAAGGGAGA AAAGGGTCCA CCGGGAGATT CAGTTTTCAC AGGTTTACAG
    ACTGGTGGCT TTGACCCTTT TGATTTGAAT TCAAAAGGAG AAAAAGGAGA GAGAGGTCTT
    CCTGGACAAG ATGGTGTTCC TGGCCTTCCA GGACGTCCTG GTCGAACTGG GCCCCCAGGG
    CCACCTGGCC TTATGGGACA AAAAGGATTT CAAGGCGATA TTGGCCCCCA AGGATATCCA
    GGTCAGCCAG GTCCCAAAGG AGACAGGGGA GAACCAGGCT ATATACTAGG AGGAGTTGAT
    GGAATGGCTG GTCTAAGTGC AATACCTGGG TCTCCTGGAC CCAAAGGACA GCCTGGGGTT
    CCAGGGCTAC CAGGACCACC TGGACTTCCA GGATTTCCTG GACCTCAGGG ACCTCCAGGA
    ACTTCTATAA AGGGGGAACC TGGTGAAACA GGGAGCCAGG GTCCTCGTGG AAAAAGTGGA
    CCAAAAGGAG ATAAAGGAGA CCAGGGGGAT GCAGGAAGAG CTGGCTTACC TGGCCCAATT
    GGGCTTGATG GCTCTCCAGG AATTCATGGA ATTAAAGGAG ACAAGGGGGA GGCTGGTATT
    GGAATCCCTG GATTACCTGG TTTAAAGGGT ATAACAGGTG AAAAGGGTGT CATTGGACTT
    CCTGGCCCTC AAGGACAGAA GGGTACTCAA GGAATACAAG GTGTAGAAGG TGTCGCAGGT
    TTAAGGGGTA AAAAAGGTCA AGATGGTGAA AAGGGTGAAA AGGGAGAACA TGGTGCTATG
    GGAATGACTG GTCCTCCTGG AATAGCTGGT CTCTCTGGAC CAATGGGGCA AAAGGGGGAT
    CAGGGGTTGC AAGGTTTTCC TGGCGCTCCT GCTATGGGGG TAATTGGGCC TTCTGGTAAA
    AAAGGTGCCA GAGGTGATAT TGGACCAGTT GGGCCAATTG GACCTAAAGG CATTATGGGA
    AGTCAAGGAG ATAAAGGAAT TAAGGGAGAA CCAGGATATG GTATACCTGG GCAGCCTGGA
    TTAAAGGGAG ATCCAGGTGA CAGAGGAAAC ATAGGATTAT CTGGAAAACC AGGTCCCAAA
    GGAGATTTTG GAGACAAAGG ACAGAAGGGT GAGCCTGGAA ACTTTGGTGC CCTAGGACAG
    TCTGGACTAA GAGGTAAAGA TGGTGAACCA GGACTAAATG GGGACAAAGG CGAGAAGGGA
    GACGCAGGTG TCCCCGGAGA ACCAGGGGAA AAGGGACAAA GGGGCTCTGT TGGACTGCCT
    GGCCGGATTG GTGATACTGG AATAAAAGGA GATATGGGGC CACCTGGAAG AGATGGCATA
    GATAGTAAAA AAGGTGATAA AGGAGAAGCC GGGCAGCCAG GAACACCTGG GGCAATGGTG
    ACTCAAATAG ACAAAAGTGT GCTTGTCAAA GGAGAAAAGG GAGAACCCGG AGAGGTGGGA
    GATCCAGGAG AATCGGGTTT TAAAGGAGAA AAAGGAGACA CGGGGCTACC AGGGCCCAGA
    GGACCTGAAG GCCGTCCAGG ACCACCTGGT GCTTCACCGT TGGAGTCCGC TTCTGAGAAA
    GGAATGAAAG GGGAAAAGGG AAGCCCAGGT TCTTCTGGAA AAAATGGCCT TGATGGAAAG
    CCTGGGAATC CTGGGTTACT TGGACAAAAG GGAGAAGCAG GCCAAAAAGG AGAACCAGGA
    AATGTAGGCC TAACTGTAAG TGAAAAGGGA AATAAAGGAG AAATAGGAAA CCAAGGCCCA
    CCAGGACCTC AGGGACCTCC TTGCACAGAT TTAGAAATGA AGGATTTCGA AAGGCTATTT
    GAATCTTATG GCATTAAGCT GTCTCTACTG AAGGAACTGA CTGATCATAT TATACAAAAT
    GGATTAGCTG ATGTAATTCA GCAACTAACA AATTCCAAGA AAGACCGAAC TTCTAAGAAG
    AAGCAAGTTT ATAAACAGTC ACCAGAATTC ACTGATGCCT TAAAACAAGA TCATATTAAT
    GAGGCAGAAA AAAACCATGA GGAAGAAGAA GATCCAAGCT TGCAGGCTGA GATAATACCT
    ACAGTGGGGG TGACTGAGTC CCATACTCTA ACTCAGGACT TAAAAGAAGG CAATAAAGAA
    CAAAAGAAAA AGAAGAAAAC ACATGTAGAG GCTGTTCCTA TTAACATTTA CACAAATGTG
    ATCACTGAGG AACAGGAGGA AATCAGCCAA GACCAGAGCC ACCATTTAAC AACCAATACT
    ACTTTTTTGC CAACTCAGCA ATCAATAGAT TTGGAAGGAT CTGATGCAAA TGCTGATAGA
    GTTGTCCAAG CACAGATTGA CAAACATCAG AGGAAGCAAG AGAAAGAGGT TAGAAAGGAA
    AGGAAAAAAA ATCGCCTTGA AACTACAGCT ACTGAAACAG AAATGAAGAA CCTTGTTTTG
    GAGGAAATTA CAGCAGTGAC TGCTTATAAA GAAGAAAATT CACAATCTGC CACCACCCTG
    CCTGAAATAA TTAATGCAAA GAGGTTAACA TCTGACTTGA TAGAGGAGAC AGACACAATC
    GTTCAGCCCA CATCTGATAC TACTATACTA GTCGAAAAGC ATCAGGAAAA GGCAGTGAAA
    GAGAGCAAGA AGGACCAGAA AAAGAAAATG CGTACTGATC ATAAAGTCAA AGAACTCAAA
    ATACAGGGAA CTGCACAACA AGAGTTAAAT CAGATACTTT CTGAGAGAAC AAGCAGGGAT
    CCCCACAGTG TTGATTCAAA TGCAGCAGCA AGCACTGAGC ATAGTTTGGA GAATCAGCAG
    GTCACCGGAA ATATATCCTT CAGGGTCAGC CTAAAAGACA ATGACTTGAC AATGATTGAT
    GAGACGTCTT GGAATGTCAC AGTAGTTGAA ACTGATAAAG CAGATCAAAC TGCATCACAG
    GAGGATGAAG AATACTTGGA GGTGCCAGAA ACTGAGACCA TCACCAGTTC CATTAACTCA
    TCAGAACATA TGGGACACAA TCGAATCAGG AGGATGGCAA AAGATTTAGA AAGGATATTC
    GCCCCAGAGA AACTAAGAAG ACAAAGGAGA ACAAAGGAAG GAAAGGGGCG TTCAGGATCT
    CAAAGAGACA AAGGACAAAA GGGTGAACCA GGGGAGGCTG GTGAGGCTGG GATTCCTGGT
    CTGAAGGGTG AAAAGGGAGA TATTGGAGAA CAGGGACAAA AGGGGGAACC TGGTGTTGGA
    CACCGTGGGC CAATTGGACA GGCTGGACCA CCAGGTGTTA AGGGTGAGCC AGGAGAGACG
    GGACCCCCAG GAGCTCAAGG AATTCAAGGT ATCCAAGGCA ACCCAGGCAT CTCAGGATTC
    CAAGGTGAAA GAGGAGAAAC AGGATTTTCA GGGCCTCCTG GACCACCTGG AGAGAGAGGC
    AAGAAGGGAA AAAATGGAAA TGCTGGTCAA CCTGGGCCCC ATGGATCAAC AGGAAAACAG
    GGGAAACCAG GACTTCAAGG TAGTAAGGGA GAAAAGGGTG AAGCTGTGGT TGGTGAACCT
    GGTCCTCGTG GGCCTCGCGG GCTTCCTGGC TTTAGGGGAG AAAATGGGAT CCCAGGCTTA
    CCTGGACCTG CGGGAATTGT AGGTTTAAAG GGATTCCCAG GAATAAAGGG TGAACGAGGT
    GATCGGGGGA TTTTTGGGAT TAAGGGAGAA AAGGGTGAAT CCACAACTCT CTTTGGCTCT
    CAAGGGTATA AAGGAAACAA AGGAGATCCG GGTGACAGAG GCTACCCAGG CTTTGATGGA
    GACAAAGGCG AGAAAGGAGA AGATGGACCT GCTGGGGAAA AAGGTGTGAA AGGTGAAGCT
    GGTTCCAAAG GATCCATGGG ACTATTTGGA GCTCGGGGTC CTGTTGGACA GAAGGGTGAG
    CCAGGGGAGC ATGGACTTCC AGGAAGAGCA GGAACAGCAG GACTTGATGG AAAAAATGGA
    CTCAAAGGAA CAAAAGGTGA CCGGGGTCTA CAAGGACAAA GGGGAAACCC GGGAGAGAAA
    GGTGATTCTG GATTGTCAGG AGATCCTGGA AAGAAAGGAG ATAAGGGTCT GCGGGGAATT
    CCAGGAAAAA TGGGAGAGCC TGGGACAGAA GGTGAAAAAG GTGACCCTGG GGCTCAAGGA
    AATCCCGGTA CAGGATCAGA AGGTCCTTCT GGACCTAAGG GTGAGAAAGG TGATAAAGGT
    GAAGATGGAC CCCCTGGAAT TGCTGGAGAT AAAGGATTTA AGGGTACTGA TGGAGTGCGT
    GGACTGAATG GCTTTAGGGG ACCAATAGGC CATCCAGGAA AAGATGGCCC TCCAGGTCCT
    ACTGGACCTT CAGGACAGAA AGGAGAGCCA GGGCCAAAGG GTGAAAGAGG AAAAAGGGGA
    AAATCCTTGC AGTGTCCAAG AGGAACCCCA GGGGATCCAG GAAACAAAGG AGAAACGGGA
    GTGGAGGGGC CTGAAGGTCT CAGAGGGGAC AAGGGAGATC CAGGGCTCAC AGAGGAAGAA
    GTGAAAGATT TGGTCAGAAA AGAAATGAGC GACAAATGTG CTTGTGCAGG AAAGGATTTT
    CATCTAGTAA TTAAATCATC ACTCCCAGAT GAAGAGTATG AAGATCCTGA CCTAAATGAA
    AGTCCAGTGA CTGCTCCACC ACAGGATATT GTGACTGAAA ATGATGTAGT AGTAGAAGCG
    TATGGAAATG TAACACTTAA TAGCACTTTA ACTCCAGATA CGGAAGTGCA ACAAAACGAC
    ACAGGTAATA GAATAATCAC AAGCATCCAT AAAATGTACA TTGTTACAAT GTCTGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54924
    Clone ID Related Accession (Same CDS sequence) XM_031899076.1 , XM_031899075.1
    Accession Version XM_031899076.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGTAGGTT TAAAGGGATT CCCAGGAATA
    AAGGGTGAAC GAGGTGATCG GGGGATTTTT GGGATTAAGG GAGAAAAGGG TGAATCCACA
    ACTCTCTTTG GCTCTCAAGG GTATAAAGGA AACAAAGGAG ATCCGGGTGA CAGAGGCTAC
    CCAGGCTTTG ATGGAGACAA AGGCGAGAAA GGAGAAGATG GACCTGCTGG GGAAAAAGGT
    GTGAAAGGTG AAGCTGGTTC CAAAGGATCC ATGGGACTAT TTGGAGCTCG GGGTCCTGTT
    GGACAGAAGG GTGAGCCAGG GGAGCATGGA CTTCCAGGAA GAGCAGGAAC AGCAGGACTT
    GATGGAAAAA ATGGACTCAA AGGAACAAAA GGTGACCGGG GTCTACAAGG ACAAAGGGGA
    AACCCGGGAG AGAAAGGTGA TTCTGGATTG TCAGGAGATC CTGGAAAGAA AGGAGATAAG
    GGTCTGCGGG GAATTCCAGG AAAAATGGGA GAGCCTGGGA CAGAAGGTGA AAAAGGTGAC
    CCTGGGGCTC AAGGAAATCC CGGTACAGGA TCAGAAGGTC CTTCTGGACC TAAGGGTGAG
    AAAGGTGATA AAGGTGAAGA TGGACCCCCT GGAATTGCTG GAGATAAAGG ATTTAAGGGT
    ACTGATGGAG TGCGTGGACT GAATGGCTTT AGGGGACCAA TAGGCCATCC AGGAAAAGAT
    GGCCCTCCAG GTCCTACTGG ACCTTCAGGA CAGAAAGGAG AGCCAGGGCC AAAGGGTGAA
    AGAGGAAAAA GGGGAAAATC CTTGCAGTGT CCAAGAGGAA CCCCAGGGGA TCCAGGAAAC
    AAAGGAGAAA CGGGAGTGGA GGGGCCTGAA GGTCTCAGAG GGGACAAGGG AGATCCAGGG
    CTCACAGAGG AAGAAGTGAA AGATTTGGTC AGAAAAGAAA TGAGCGACAA ATGTGCTTGT
    GCAGGAAAGG ATTTTCATCT AGTAATTAAA TCATCACTCC CAGATGAAGA GTATGAAGAT
    CCTGACCTAA ATGAAAGTCC AGTGACTGCT CCACCACAGG ATATTGTGAC TGAAAATGAT
    GTAGTAGTAG AAGCGTATGG AAATGTAACA CTTAATAGCA CTTTAACTCC AGATACGGAA
    GTGCAACAAA ACGACACAGA CGGAAGTGAT AAGGAGCTAA TGGGACAATT GCAGAGAAGA
    AAACGTGAAG CAGAAAATCT GTTTCGTAAT CCCTGTTTGC TTCCAATGGA TGAAGGATTC
    TGCTCCGATT ACACCTTGGT TTGGTATTAC CACCGCAAAA CAGACGTATG CCGGCCTTTT
    TTATTTGGTG GTTGTGGGGG AAATATCAAT CGCTTTAAAA CAAAAGACAG ATGTGAGCAG
    AGGTGTAAAA CTCGATCAGT ATCATCCCGA GGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754936.1
    CDS1384..9822
    Translation

    Target ORF information:

    RefSeq Version XM_031899076.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGTAGGTT TAAAGGGATT CCCAGGAATA
    AAGGGTGAAC GAGGTGATCG GGGGATTTTT GGGATTAAGG GAGAAAAGGG TGAATCCACA
    ACTCTCTTTG GCTCTCAAGG GTATAAAGGA AACAAAGGAG ATCCGGGTGA CAGAGGCTAC
    CCAGGCTTTG ATGGAGACAA AGGCGAGAAA GGAGAAGATG GACCTGCTGG GGAAAAAGGT
    GTGAAAGGTG AAGCTGGTTC CAAAGGATCC ATGGGACTAT TTGGAGCTCG GGGTCCTGTT
    GGACAGAAGG GTGAGCCAGG GGAGCATGGA CTTCCAGGAA GAGCAGGAAC AGCAGGACTT
    GATGGAAAAA ATGGACTCAA AGGAACAAAA GGTGACCGGG GTCTACAAGG ACAAAGGGGA
    AACCCGGGAG AGAAAGGTGA TTCTGGATTG TCAGGAGATC CTGGAAAGAA AGGAGATAAG
    GGTCTGCGGG GAATTCCAGG AAAAATGGGA GAGCCTGGGA CAGAAGGTGA AAAAGGTGAC
    CCTGGGGCTC AAGGAAATCC CGGTACAGGA TCAGAAGGTC CTTCTGGACC TAAGGGTGAG
    AAAGGTGATA AAGGTGAAGA TGGACCCCCT GGAATTGCTG GAGATAAAGG ATTTAAGGGT
    ACTGATGGAG TGCGTGGACT GAATGGCTTT AGGGGACCAA TAGGCCATCC AGGAAAAGAT
    GGCCCTCCAG GTCCTACTGG ACCTTCAGGA CAGAAAGGAG AGCCAGGGCC AAAGGGTGAA
    AGAGGAAAAA GGGGAAAATC CTTGCAGTGT CCAAGAGGAA CCCCAGGGGA TCCAGGAAAC
    AAAGGAGAAA CGGGAGTGGA GGGGCCTGAA GGTCTCAGAG GGGACAAGGG AGATCCAGGG
    CTCACAGAGG AAGAAGTGAA AGATTTGGTC AGAAAAGAAA TGAGCGACAA ATGTGCTTGT
    GCAGGAAAGG ATTTTCATCT AGTAATTAAA TCATCACTCC CAGATGAAGA GTATGAAGAT
    CCTGACCTAA ATGAAAGTCC AGTGACTGCT CCACCACAGG ATATTGTGAC TGAAAATGAT
    GTAGTAGTAG AAGCGTATGG AAATGTAACA CTTAATAGCA CTTTAACTCC AGATACGGAA
    GTGCAACAAA ACGACACAGA CGGAAGTGAT AAGGAGCTAA TGGGACAATT GCAGAGAAGA
    AAACGTGAAG CAGAAAATCT GTTTCGTAAT CCCTGTTTGC TTCCAATGGA TGAAGGATTC
    TGCTCCGATT ACACCTTGGT TTGGTATTAC CACCGCAAAA CAGACGTATG CCGGCCTTTT
    TTATTTGGTG GTTGTGGGGG AAATATCAAT CGCTTTAAAA CAAAAGACAG ATGTGAGCAG
    AGGTGTAAAA CTCGATCAGT ATCATCCCGA GGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54924
    Clone ID Related Accession (Same CDS sequence) XM_031899076.1 , XM_031899075.1
    Accession Version XM_031899075.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGTAGGTT TAAAGGGATT CCCAGGAATA
    AAGGGTGAAC GAGGTGATCG GGGGATTTTT GGGATTAAGG GAGAAAAGGG TGAATCCACA
    ACTCTCTTTG GCTCTCAAGG GTATAAAGGA AACAAAGGAG ATCCGGGTGA CAGAGGCTAC
    CCAGGCTTTG ATGGAGACAA AGGCGAGAAA GGAGAAGATG GACCTGCTGG GGAAAAAGGT
    GTGAAAGGTG AAGCTGGTTC CAAAGGATCC ATGGGACTAT TTGGAGCTCG GGGTCCTGTT
    GGACAGAAGG GTGAGCCAGG GGAGCATGGA CTTCCAGGAA GAGCAGGAAC AGCAGGACTT
    GATGGAAAAA ATGGACTCAA AGGAACAAAA GGTGACCGGG GTCTACAAGG ACAAAGGGGA
    AACCCGGGAG AGAAAGGTGA TTCTGGATTG TCAGGAGATC CTGGAAAGAA AGGAGATAAG
    GGTCTGCGGG GAATTCCAGG AAAAATGGGA GAGCCTGGGA CAGAAGGTGA AAAAGGTGAC
    CCTGGGGCTC AAGGAAATCC CGGTACAGGA TCAGAAGGTC CTTCTGGACC TAAGGGTGAG
    AAAGGTGATA AAGGTGAAGA TGGACCCCCT GGAATTGCTG GAGATAAAGG ATTTAAGGGT
    ACTGATGGAG TGCGTGGACT GAATGGCTTT AGGGGACCAA TAGGCCATCC AGGAAAAGAT
    GGCCCTCCAG GTCCTACTGG ACCTTCAGGA CAGAAAGGAG AGCCAGGGCC AAAGGGTGAA
    AGAGGAAAAA GGGGAAAATC CTTGCAGTGT CCAAGAGGAA CCCCAGGGGA TCCAGGAAAC
    AAAGGAGAAA CGGGAGTGGA GGGGCCTGAA GGTCTCAGAG GGGACAAGGG AGATCCAGGG
    CTCACAGAGG AAGAAGTGAA AGATTTGGTC AGAAAAGAAA TGAGCGACAA ATGTGCTTGT
    GCAGGAAAGG ATTTTCATCT AGTAATTAAA TCATCACTCC CAGATGAAGA GTATGAAGAT
    CCTGACCTAA ATGAAAGTCC AGTGACTGCT CCACCACAGG ATATTGTGAC TGAAAATGAT
    GTAGTAGTAG AAGCGTATGG AAATGTAACA CTTAATAGCA CTTTAACTCC AGATACGGAA
    GTGCAACAAA ACGACACAGA CGGAAGTGAT AAGGAGCTAA TGGGACAATT GCAGAGAAGA
    AAACGTGAAG CAGAAAATCT GTTTCGTAAT CCCTGTTTGC TTCCAATGGA TGAAGGATTC
    TGCTCCGATT ACACCTTGGT TTGGTATTAC CACCGCAAAA CAGACGTATG CCGGCCTTTT
    TTATTTGGTG GTTGTGGGGG AAATATCAAT CGCTTTAAAA CAAAAGACAG ATGTGAGCAG
    AGGTGTAAAA CTCGATCAGT ATCATCCCGA GGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754935.1
    CDS1388..9826
    Translation

    Target ORF information:

    RefSeq Version XM_031899075.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899075.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGTAGGTT TAAAGGGATT CCCAGGAATA
    AAGGGTGAAC GAGGTGATCG GGGGATTTTT GGGATTAAGG GAGAAAAGGG TGAATCCACA
    ACTCTCTTTG GCTCTCAAGG GTATAAAGGA AACAAAGGAG ATCCGGGTGA CAGAGGCTAC
    CCAGGCTTTG ATGGAGACAA AGGCGAGAAA GGAGAAGATG GACCTGCTGG GGAAAAAGGT
    GTGAAAGGTG AAGCTGGTTC CAAAGGATCC ATGGGACTAT TTGGAGCTCG GGGTCCTGTT
    GGACAGAAGG GTGAGCCAGG GGAGCATGGA CTTCCAGGAA GAGCAGGAAC AGCAGGACTT
    GATGGAAAAA ATGGACTCAA AGGAACAAAA GGTGACCGGG GTCTACAAGG ACAAAGGGGA
    AACCCGGGAG AGAAAGGTGA TTCTGGATTG TCAGGAGATC CTGGAAAGAA AGGAGATAAG
    GGTCTGCGGG GAATTCCAGG AAAAATGGGA GAGCCTGGGA CAGAAGGTGA AAAAGGTGAC
    CCTGGGGCTC AAGGAAATCC CGGTACAGGA TCAGAAGGTC CTTCTGGACC TAAGGGTGAG
    AAAGGTGATA AAGGTGAAGA TGGACCCCCT GGAATTGCTG GAGATAAAGG ATTTAAGGGT
    ACTGATGGAG TGCGTGGACT GAATGGCTTT AGGGGACCAA TAGGCCATCC AGGAAAAGAT
    GGCCCTCCAG GTCCTACTGG ACCTTCAGGA CAGAAAGGAG AGCCAGGGCC AAAGGGTGAA
    AGAGGAAAAA GGGGAAAATC CTTGCAGTGT CCAAGAGGAA CCCCAGGGGA TCCAGGAAAC
    AAAGGAGAAA CGGGAGTGGA GGGGCCTGAA GGTCTCAGAG GGGACAAGGG AGATCCAGGG
    CTCACAGAGG AAGAAGTGAA AGATTTGGTC AGAAAAGAAA TGAGCGACAA ATGTGCTTGT
    GCAGGAAAGG ATTTTCATCT AGTAATTAAA TCATCACTCC CAGATGAAGA GTATGAAGAT
    CCTGACCTAA ATGAAAGTCC AGTGACTGCT CCACCACAGG ATATTGTGAC TGAAAATGAT
    GTAGTAGTAG AAGCGTATGG AAATGTAACA CTTAATAGCA CTTTAACTCC AGATACGGAA
    GTGCAACAAA ACGACACAGA CGGAAGTGAT AAGGAGCTAA TGGGACAATT GCAGAGAAGA
    AAACGTGAAG CAGAAAATCT GTTTCGTAAT CCCTGTTTGC TTCCAATGGA TGAAGGATTC
    TGCTCCGATT ACACCTTGGT TTGGTATTAC CACCGCAAAA CAGACGTATG CCGGCCTTTT
    TTATTTGGTG GTTGTGGGGG AAATATCAAT CGCTTTAAAA CAAAAGACAG ATGTGAGCAG
    AGGTGTAAAA CTCGATCAGT ATCATCCCGA GGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54925
    Clone ID Related Accession (Same CDS sequence) XM_031899077.1
    Accession Version XM_031899077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GAAAAAGTAA TTGGCCCTAA AGATCTGATT
    ATAAGTGAGG AAAGTTACAA CTCTTTGCGT CTCACTTGGA CTGCAGCCAC AGGAGATGCT
    ACTGGATATC AAGTTGTCTT GAATTCCCTT ACAGATTCAG GGCATCTTAA TTCCCATGAC
    CAAAAGCAGA TTGTTGTGGA CAAGGACAAG CATACTATTA TTGTGTCGGA TCTAAGGCCA
    AACACAGAAT ATTTATTTAC AGTACTTGCT CTCTACTCTA ATTTCATAGG GGATTCAGCC
    ACAATTCGTG GAAGGACAAC TCCTATTCCA CCTGTGACAA ATTTTCGAGT CATTGAAGAG
    GGACTTTTTA GCTTAAAGCT GGCCTGGACT CCTTCTCTGG GGAAACTGGA TGGTTATAAG
    ATCTTTATTC CAAGATCCAA CAGACCTGGA TTAACATATG AACAGATTGT GAGTGGAGAT
    GTCTTCTCAC ACACTATTGA CAATCTAGAG GAAGATAAAG AATACACTGT CAGTATATAT
    GCTGTATATC CAGAAGGACC CAGTAACCCT GTCTCAGCAG TTGGACACAC ATTAAAGCTG
    CTTGCTGTGA AAAGCCTCTC CCTGCAGAAC ATCACAACAG GCACTATACG AGCACAATGG
    ACTACAGTAC ATGGAGCAAC TGGGTATCGA CTGACTTGGT CCTCTGCAGA GGGCAATGAC
    CAAAATGTTA ACCTAGCTGA TAGCTATTCC CAGTATATGA TACAAGGGCT TCAGGCGGAC
    ACCGAGTACA CAGTGACGAT TAATCCTATC TTTGTGGACA TAGAGGGTCC TGTTGTAACA
    GCAAAAGCTA CAACCTTGGC ATCTAGTGCA GTACAAAGTC TTAAAATAAG TGCAGTAACT
    ATAAGCACTG CAGTGGTGTC ATGGAATGCT GTGCCAGGAG CTACAGGATA CAGACTTGCA
    TGGGGTCCCA CACCAGAATT TATTGGTAAG GACCGTCCTC GCCAGCTAGC CCTCAATAGT
    TCAATCACAA CCTACCAACT GAAACACCTT GTTCACAATA CGGAATATGT CCTTTCATTA
    TATGTACTCT TTGGCTCTGT CGTAGGGCCT GGAATTTCAA CCACACTCAG GACATCTCCA
    CTTGGATATG TTTCTAACTT TAAGGTGACA GGATATACGA GTACCTCTAT CTCGCTTGCT
    TGGAGTTACA CTATTGGAGC TACTGAATAT AAAATTTCCT GGAGCACCAC AACATCAGGT
    TTGCCACTGA ACACTCCTAA AAGTCATTAT CTGGCGTCCG GTGTCAGGTC CTATCACATC
    ACAAATTTGT ACCCAAACAC AAAGTACACT GTGAATGTTC AGGCAGTATA TAATAACTTT
    GAAGGGCCGC CAAGCACTCT AATTCAAACC ACAGACTCAA ATCCTGTGCA GCTACTGCCT
    GTGAAAGATC TGCAGATCAT TGATACTGGA GTCAATATGC TGAAACTGTC TTGGAAGAAA
    ACACCTGGAA TAACACATTA TAAGATCTCA TGGACACCAT TTGATGGTGG TGCAAGAAAC
    TCTAAGATTG TCTCTGCTGG TACGTCATTT TTCACAATCC CTGACCTTAA GGAGAGTTCC
    ACTTACAGCA TCTATGTATC CTCGATAATC GGGAATCGGG AAGGAAGTTC AGTGGTTCTT
    ACTGCAAAAA CATTGGATTT GCCAAAAGTA GAATCTTTTG AAGTCAAAGA GACCACAGAG
    GACAGTGTTT TATTAAGCTG GACTGGAGTC ACTGGAGCAT CTTCATATAT TTTAACATGG
    AGGATGGCTT CAGACCCAGA ATCCTCCGTG GAGCAGTTAG CTCCAACATA TAGATCTTTC
    CGGCTCATGA ATTTGGAGAA TGGAAAGTCA TATATTTTTG CAATAAAGCC TCTTTTTGGA
    GAGATGGAAG GGCCTGAGAG AACAATCACA CACACAATCG TTGGACCCCC CAAAAAACAG
    ATCTACCCTT CCCCTACCCT TCAAATACCT GTTCCAACAC CTCAAAGGAC CCTAACAACA
    CAAGTTATCA ACCACCCTCC CATAGTCACC ACAACTGAAG CTGCAAAATC CACAAGCACT
    GTCTCACATG GTTCTTCCAC AACTACTTCT GAACCCATTT GTGCAAAACT GAAAGCTGAC
    ATAGTATTTC TGGTTGATGA GTCCTCCAGC ATTGGCCCAT CCAATTTTAT TAAGGTTAAG
    GATTTCCTCT ACAGAATTGT CTCTTACTTT CCCAAAATTG GACCTCAAGG AACACAGATT
    GCTATTGTTC AGTACAGCGA AGAACCCAGA ACAGAATTCC ACTTGAATGA GTATAAGGAG
    AGAAATTCTG TTTTGAAAGC AATCAGAGCT CTACAGTATT ATGGAGGCAA CACCAAGACT
    GGTCGTGGTA TAGGACATGC ACTGAAGGAA ATGTTTCAGG TTTCCAAAGG GATGAGACCA
    TCTGCTCCAC ATATACTTGT GCTAATAACT GACGGAAGGT CTCAGGATAA TGTAATACAG
    CCAGCCAAAG TAGCTCATGT ATTAGGCATT CGTATGATTG CAGTCGGTGT GGCTGGGGCA
    GATATGGATG AACTGAAAGG AATGATGATG AACCGTAATT TAGAAAACAT CTTCTTTGCC
    AGTACATTTG ATGATTTTCC TCTAATTGTA CAAGATCTGA TAGATGCTAT TTGCACTGAA
    ACCGAACATG CAGTTAAAGA AGAACCTACT AAGTTCAGAG CACCAGATAA AGAAGAGGAG
    CCAGAAAGAC CAAAACCTCC TCCTGGGAAT CGAGAGGGAC CCTGTGGAAA TACCATGTGC
    CCAACGGGGC AAAAGGGACA AAAGGGAGAA AAGGGTCCAC CGGGAGATTC AGTTTTCACA
    GGTTTACAGA CTGGTGGCTT TGACCCTTTT GATTTGAATT CAAAAGGAGA AAAAGGAGAG
    AGAGGTCTTC CTGGACAAGA TGGTGTTCCT GGCCTTCCAG GACGTCCTGG TCGAACTGGG
    CCCCCAGGGC CACCTGGCCT TATGGGACAA AAAGGATTTC AAGGCGATAT TGGCCCCCAA
    GGATATCCAG GTCAGCCAGG TCCCAAAGGA GACAGGGGAG AACCAGGCTA TATACTAGGA
    GGAGTTGATG GAATGGCTGG TCTAAGTGCA ATACCTGGGT CTCCTGGACC CAAAGGACAG
    CCTGGGGTTC CAGGGCTACC AGGACCACCT GGACTTCCAG GATTTCCTGG ACCTCAGGGA
    CCTCCAGGAA CTTCTATAAA GGGGGAACCT GGTGAAACAG GGAGCCAGGG TCCTCGTGGA
    AAAAGTGGAC CAAAAGGAGA TAAAGGAGAC CAGGGGGATG CAGGAAGAGC TGGCTTACCT
    GGCCCAATTG GGCTTGATGG CTCTCCAGGA ATTCATGGAA TTAAAGGAGA CAAGGGGGAG
    GCTGGTATTG GAATCCCTGG ATTACCTGGT TTAAAGGGTA TAACAGGTGA AAAGGGTGTC
    ATTGGACTTC CTGGCCCTCA AGGACAGAAG GGTACTCAAG GAATACAAGG TGTAGAAGGT
    GTCGCAGGTT TAAGGGGTAA AAAAGGTCAA GATGGTGAAA AGGGTGAAAA GGGAGAACAT
    GGTGCTATGG GAATGACTGG TCCTCCTGGA ATAGCTGGTC TCTCTGGACC AATGGGGCAA
    AAGGGGGATC AGGGGTTGCA AGGTTTTCCT GGCGCTCCTG CTATGGGGGT AATTGGGCCT
    TCTGGTAAAA AAGGTGCCAG AGGTGATATT GGACCAGTTG GGCCAATTGG ACCTAAAGGC
    ATTATGGGAA GTCAAGGAGA TAAAGGAATT AAGGGAGAAC CAGGATATGG TATACCTGGG
    CAGCCTGGAT TAAAGGGAGA TCCAGGTGAC AGAGGAAACA TAGGATTATC TGGAAAACCA
    GGTCCCAAAG GAGATTTTGG AGACAAAGGA CAGAAGGGTG AGCCTGGAAA CTTTGGTGCC
    CTAGGACAGT CTGGACTAAG AGGTAAAGAT GGTGAACCAG GACTAAATGG GGACAAAGGC
    GAGAAGGGAG ACGCAGGTGT CCCCGGAGAA CCAGGGGAAA AGGGACAAAG GGGCTCTGTT
    GGACTGCCTG GCCGGATTGG TGATACTGGA ATAAAAGGAG ATATGGGGCC ACCTGGAAGA
    GATGGCATAG ATAGTAAAAA AGGTGATAAA GGAGAAGCCG GGCAGCCAGG AACACCTGGG
    GCAATGGTGA CTCAAATAGA CAAAAGTGTG CTTGTCAAAG GAGAAAAGGG AGAACCCGGA
    GAGGTGGGAG ATCCAGGAGA ATCGGGTTTT AAAGGAGAAA AAGGAGACAC GGGGCTACCA
    GGGCCCAGAG GACCTGAAGG CCGTCCAGGA CCACCTGGTG CTTCACCGTT GGAGTCCGCT
    TCTGAGAAAG GAATGAAAGG GGAAAAGGGA AGCCCAGGTT CTTCTGGAAA AAATGGCCTT
    GATGGAAAGC CTGGGAATCC TGGGTTACTT GGACAAAAGG GAGAAGCAGG CCAAAAAGGA
    GAACCAGGAA ATGTAGGCCT AACTGTAAGT GAAAAGGGAA ATAAAGGAGA AATAGGAAAC
    CAAGGCCCAC CAGGACCTCA GGGACCTCCT TGCACAGATT TAGAAATGAA GGATTTCGAA
    AGGCTATTTG AATCTTATGG CATTAAGCTG TCTCTACTGA AGGAACTGAC TGATCATATT
    ATACAAAATG GATTAGCTGA TGTAATTCAG CAACTAACAA ATTCCAAGAA AGACCGAACT
    TCTAAGAAGA AGCAAGTTTA TAAACAGTCA CCAGAATTCA CTGATGCCTT AAAACAAGAT
    CATATTAATG AGGCAGAAAA AAACCATGAG GAAGAAGAAG ATCCAAGCTT GCAGGCTGAG
    ATAATACCTA CAGTGGGGGT GACTGAGTCC CATACTCTAA CTCAGGACTT AAAAGAAGGC
    AATAAAGAAC AAAAGAAAAA GAAGAAAACA CATGTAGAGG CTGTTCCTAT TAACATTTAC
    ACAAATGTGA TCACTGAGGA ACAGGAGGAA ATCAGCCAAG ACCAGAGCCA CCATTTAACA
    ACCAATACTA CTTTTTTGCC AACTCAGCAA TCAATAGATT TGGAAGGATC TGATGCAAAT
    GCTGATAGAG TTGTCCAAGC ACAGATTGAC AAACATCAGA GGAAGCAAGA GAAAGAGGTT
    AGAAAGGAAA GGAAAAAAAA TCGCCTTGAA ACTACAGCTA CTGAAACAGA AATGAAGAAC
    CTTGTTTTGG AGGAAATTAC AGCAGTGACT GCTTATAAAG AAGAAAATTC ACAATCTGCC
    ACCACCCTGC CTGCAATAAT TAATGCAAAG AGGTTAACAT CTGACTTGAT AGAGGAGACA
    GACACAATCG TTCAGCCCAC ATCTGATACT ACTATACTAG TCGAAAAGCA TCAGGAAAAG
    GCAGTGAAAG AGAGCAAGAA GGACCAGAAA AAGAAAATGC GTACTGATCA TAAAGTCAAA
    GAACTCAAAA TACAGGGAAC TGCACAACAA GAGTTAAATC AGATACTTTC TGAGAGAACA
    AGCAGGGATC CCCACAGTGT TGATTCAAAT GCAGCAGCAA GCACTGAGCA TAGTTTGGAG
    AATCAGCAGG TCACCGGAAA TATATCCTTC AGGGTCAGCC TAAAAGACAA TGACTTGACA
    ATGATTGATG AGACGTCTTG GAATGTCACA GTAGTTGAAA CTGATAAAGC AGATCAAACT
    GCATCACAGG AGGATGAAGA ATACTTGGAG GTGCCAGAAA CTGAGCAGAC CATCACCAGT
    TCCATTAACT CATCAGAACA TATGGTACAC AATAGAATCA GGAGGATGGC AAAAGATTTA
    GAAAGGATAT TCGCCCCAGA GAAACTAAGA AGACAAAGGA GAACAAAGGA AGGAAAGGGG
    CGTTCAGGAT CTCAAAGAGA CAAAGGACAA AAGGGTGAAC CGGGGGAGGC TGGTGAGGCT
    GGGATTCCTG GTCTGAAGGG TGAAAAGGGA GATATTGGAG AACAGGGACA AAAGGGGGAA
    CCTGGTGTTG GACACCGTGG GCCAATTGGA CAGGCTGGAC CACCAGGTGT TAAGGGTGAG
    CCAGGAGAGA CGGGACCCCC AGGAGCTCAA GGAATTCAAG GTATCCAAGG CAACCCAGGC
    ATCTCAGGAT TCCAAGGTGA AAGAGGAGAA ACAGGATTTT CAGGGCCTCC TGGACCACCT
    GGAGAGAGAG GCAAGAAGGG AAAAAATGGA AATGCTGGTC AACCTGGGCC CCATGGATCA
    ACAGGAAAAC AGGGGAAACC AGGACTTCAA GGTAGTAAGG GAGAAAAGGG TGAAGCTGTG
    GTTGGTGAAC CTGGTCCTCG TGGGCCTCGC GGGCTTCCTG GCTTTAGGGG AGAAAATGGG
    ATCCCAGGCT TACCTGGACC TGCGGGAATT GTAGGTTTAA AGGGATTCCC AGGAATAAAG
    GGTGAACGAG GTGATCGGGG GATTTTTGGG ATTAAGGGAG AAAAGGGTGA ATCCACAACT
    CTCTTTGGCT CTCAAGGGTA TAAAGGAAAC AAAGGAGATC CGGGTGACAG AGGCTACCCA
    GGCTTTGATG GAGACAAAGG CGAGAAAGGA GAAGATGGAC CTGCTGGGGA AAAAGGTGTG
    AAAGGTGAAG CTGGTTCCAA AGGATCCATG GGACTATTTG GAGCTCGGGG TCCTGTTGGA
    CAGAAGGGTG AGCCAGGGGA GCATGGACTT CCAGGAAGAG CAGGAACAGC AGGACTTGAT
    GGAAAAAATG GACTCAAAGG AACAAAAGGT GACCGGGGTC TACAAGGACA AAGGGGAAAC
    CCGGGAGAGA AAGGTGATTC TGGATTGTCA GGAGATCCTG GAAAGAAAGG AGATAAGGGT
    CTGCGGGGAA TTCCAGGAAA AATGGGAGAG CCTGGGACAG AAGGTGAAAA AGGTGACCCT
    GGGGCTCAAG GAAATCCCGG TACAGGATCA GAAGGTCCTT CTGGACCTAA GGGTGAGAAA
    GGTGATAAAG GTGAAGATGG ACCCCCTGGA ATTGCTGGAG ATAAAGGATT TAAGGGTACT
    GATGGAGTGC GTGGACTGAA TGGCTTTAGG GGACCAATAG GCCATCCAGG AAAAGATGGC
    CCTCCAGGTC CTACTGGACC TTCAGGACAG AAAGGAGAGC CAGGGCCAAA GGGTGAAAGA
    GGAAAAAGGG GAAAATCCTT GCAGTGTCCA AGAGGAACCC CAGGGGATCC AGGAAACAAA
    GGAGAAACGG GAGTGGAGGG GCCTGAAGGT CTCAGAGGGG ACAAGGGAGA TCCAGGGCTC
    ACAGAGGAAG AAGTGAAAGA TTTGGTCAGA AAAGAAATGA GCGACAAATG TGCTTGTGCA
    GGAAAGGATT TTCATCTAGT AATTAAATCA TCACTCCCAG ATGAAGAGTA TGAAGATCCT
    GACCTAAATG AAAGTCCAGT GACTGCTCCA CCACAGGATA TTGTGACTGA AAATGATGTA
    GTAGTAGAAG CGTATGGAAA TGTAACACTT AATAGCACTT TAACTCCAGA TACGGAAGTG
    CAACAAAACG ACACAGACGG AAGTGATAAG GAGCTAATGG GACAATTGCA GAGAAGAAAA
    CGTGAAGCAG AAAATCTGTT TCGTAATCCC TGTTTGCTTC CAATGGATGA AGGATTCTGC
    TCCGATTACA CCTTGGTTTG GTATTACCAC CGCAAAACAG ACGTATGCCG GCCTTTTTTA
    TTTGGTGGTT GTGGGGGAAA TATCAATCGC TTTAAAACAA AAGACAGATG TGAGCAGAGG
    TGTAAAACTC GATCAGTATC ATCCCGAGGG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754937.1
    CDS1388..9823
    Translation

    Target ORF information:

    RefSeq Version XM_031899077.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GAAAAAGTAA TTGGCCCTAA AGATCTGATT
    ATAAGTGAGG AAAGTTACAA CTCTTTGCGT CTCACTTGGA CTGCAGCCAC AGGAGATGCT
    ACTGGATATC AAGTTGTCTT GAATTCCCTT ACAGATTCAG GGCATCTTAA TTCCCATGAC
    CAAAAGCAGA TTGTTGTGGA CAAGGACAAG CATACTATTA TTGTGTCGGA TCTAAGGCCA
    AACACAGAAT ATTTATTTAC AGTACTTGCT CTCTACTCTA ATTTCATAGG GGATTCAGCC
    ACAATTCGTG GAAGGACAAC TCCTATTCCA CCTGTGACAA ATTTTCGAGT CATTGAAGAG
    GGACTTTTTA GCTTAAAGCT GGCCTGGACT CCTTCTCTGG GGAAACTGGA TGGTTATAAG
    ATCTTTATTC CAAGATCCAA CAGACCTGGA TTAACATATG AACAGATTGT GAGTGGAGAT
    GTCTTCTCAC ACACTATTGA CAATCTAGAG GAAGATAAAG AATACACTGT CAGTATATAT
    GCTGTATATC CAGAAGGACC CAGTAACCCT GTCTCAGCAG TTGGACACAC ATTAAAGCTG
    CTTGCTGTGA AAAGCCTCTC CCTGCAGAAC ATCACAACAG GCACTATACG AGCACAATGG
    ACTACAGTAC ATGGAGCAAC TGGGTATCGA CTGACTTGGT CCTCTGCAGA GGGCAATGAC
    CAAAATGTTA ACCTAGCTGA TAGCTATTCC CAGTATATGA TACAAGGGCT TCAGGCGGAC
    ACCGAGTACA CAGTGACGAT TAATCCTATC TTTGTGGACA TAGAGGGTCC TGTTGTAACA
    GCAAAAGCTA CAACCTTGGC ATCTAGTGCA GTACAAAGTC TTAAAATAAG TGCAGTAACT
    ATAAGCACTG CAGTGGTGTC ATGGAATGCT GTGCCAGGAG CTACAGGATA CAGACTTGCA
    TGGGGTCCCA CACCAGAATT TATTGGTAAG GACCGTCCTC GCCAGCTAGC CCTCAATAGT
    TCAATCACAA CCTACCAACT GAAACACCTT GTTCACAATA CGGAATATGT CCTTTCATTA
    TATGTACTCT TTGGCTCTGT CGTAGGGCCT GGAATTTCAA CCACACTCAG GACATCTCCA
    CTTGGATATG TTTCTAACTT TAAGGTGACA GGATATACGA GTACCTCTAT CTCGCTTGCT
    TGGAGTTACA CTATTGGAGC TACTGAATAT AAAATTTCCT GGAGCACCAC AACATCAGGT
    TTGCCACTGA ACACTCCTAA AAGTCATTAT CTGGCGTCCG GTGTCAGGTC CTATCACATC
    ACAAATTTGT ACCCAAACAC AAAGTACACT GTGAATGTTC AGGCAGTATA TAATAACTTT
    GAAGGGCCGC CAAGCACTCT AATTCAAACC ACAGACTCAA ATCCTGTGCA GCTACTGCCT
    GTGAAAGATC TGCAGATCAT TGATACTGGA GTCAATATGC TGAAACTGTC TTGGAAGAAA
    ACACCTGGAA TAACACATTA TAAGATCTCA TGGACACCAT TTGATGGTGG TGCAAGAAAC
    TCTAAGATTG TCTCTGCTGG TACGTCATTT TTCACAATCC CTGACCTTAA GGAGAGTTCC
    ACTTACAGCA TCTATGTATC CTCGATAATC GGGAATCGGG AAGGAAGTTC AGTGGTTCTT
    ACTGCAAAAA CATTGGATTT GCCAAAAGTA GAATCTTTTG AAGTCAAAGA GACCACAGAG
    GACAGTGTTT TATTAAGCTG GACTGGAGTC ACTGGAGCAT CTTCATATAT TTTAACATGG
    AGGATGGCTT CAGACCCAGA ATCCTCCGTG GAGCAGTTAG CTCCAACATA TAGATCTTTC
    CGGCTCATGA ATTTGGAGAA TGGAAAGTCA TATATTTTTG CAATAAAGCC TCTTTTTGGA
    GAGATGGAAG GGCCTGAGAG AACAATCACA CACACAATCG TTGGACCCCC CAAAAAACAG
    ATCTACCCTT CCCCTACCCT TCAAATACCT GTTCCAACAC CTCAAAGGAC CCTAACAACA
    CAAGTTATCA ACCACCCTCC CATAGTCACC ACAACTGAAG CTGCAAAATC CACAAGCACT
    GTCTCACATG GTTCTTCCAC AACTACTTCT GAACCCATTT GTGCAAAACT GAAAGCTGAC
    ATAGTATTTC TGGTTGATGA GTCCTCCAGC ATTGGCCCAT CCAATTTTAT TAAGGTTAAG
    GATTTCCTCT ACAGAATTGT CTCTTACTTT CCCAAAATTG GACCTCAAGG AACACAGATT
    GCTATTGTTC AGTACAGCGA AGAACCCAGA ACAGAATTCC ACTTGAATGA GTATAAGGAG
    AGAAATTCTG TTTTGAAAGC AATCAGAGCT CTACAGTATT ATGGAGGCAA CACCAAGACT
    GGTCGTGGTA TAGGACATGC ACTGAAGGAA ATGTTTCAGG TTTCCAAAGG GATGAGACCA
    TCTGCTCCAC ATATACTTGT GCTAATAACT GACGGAAGGT CTCAGGATAA TGTAATACAG
    CCAGCCAAAG TAGCTCATGT ATTAGGCATT CGTATGATTG CAGTCGGTGT GGCTGGGGCA
    GATATGGATG AACTGAAAGG AATGATGATG AACCGTAATT TAGAAAACAT CTTCTTTGCC
    AGTACATTTG ATGATTTTCC TCTAATTGTA CAAGATCTGA TAGATGCTAT TTGCACTGAA
    ACCGAACATG CAGTTAAAGA AGAACCTACT AAGTTCAGAG CACCAGATAA AGAAGAGGAG
    CCAGAAAGAC CAAAACCTCC TCCTGGGAAT CGAGAGGGAC CCTGTGGAAA TACCATGTGC
    CCAACGGGGC AAAAGGGACA AAAGGGAGAA AAGGGTCCAC CGGGAGATTC AGTTTTCACA
    GGTTTACAGA CTGGTGGCTT TGACCCTTTT GATTTGAATT CAAAAGGAGA AAAAGGAGAG
    AGAGGTCTTC CTGGACAAGA TGGTGTTCCT GGCCTTCCAG GACGTCCTGG TCGAACTGGG
    CCCCCAGGGC CACCTGGCCT TATGGGACAA AAAGGATTTC AAGGCGATAT TGGCCCCCAA
    GGATATCCAG GTCAGCCAGG TCCCAAAGGA GACAGGGGAG AACCAGGCTA TATACTAGGA
    GGAGTTGATG GAATGGCTGG TCTAAGTGCA ATACCTGGGT CTCCTGGACC CAAAGGACAG
    CCTGGGGTTC CAGGGCTACC AGGACCACCT GGACTTCCAG GATTTCCTGG ACCTCAGGGA
    CCTCCAGGAA CTTCTATAAA GGGGGAACCT GGTGAAACAG GGAGCCAGGG TCCTCGTGGA
    AAAAGTGGAC CAAAAGGAGA TAAAGGAGAC CAGGGGGATG CAGGAAGAGC TGGCTTACCT
    GGCCCAATTG GGCTTGATGG CTCTCCAGGA ATTCATGGAA TTAAAGGAGA CAAGGGGGAG
    GCTGGTATTG GAATCCCTGG ATTACCTGGT TTAAAGGGTA TAACAGGTGA AAAGGGTGTC
    ATTGGACTTC CTGGCCCTCA AGGACAGAAG GGTACTCAAG GAATACAAGG TGTAGAAGGT
    GTCGCAGGTT TAAGGGGTAA AAAAGGTCAA GATGGTGAAA AGGGTGAAAA GGGAGAACAT
    GGTGCTATGG GAATGACTGG TCCTCCTGGA ATAGCTGGTC TCTCTGGACC AATGGGGCAA
    AAGGGGGATC AGGGGTTGCA AGGTTTTCCT GGCGCTCCTG CTATGGGGGT AATTGGGCCT
    TCTGGTAAAA AAGGTGCCAG AGGTGATATT GGACCAGTTG GGCCAATTGG ACCTAAAGGC
    ATTATGGGAA GTCAAGGAGA TAAAGGAATT AAGGGAGAAC CAGGATATGG TATACCTGGG
    CAGCCTGGAT TAAAGGGAGA TCCAGGTGAC AGAGGAAACA TAGGATTATC TGGAAAACCA
    GGTCCCAAAG GAGATTTTGG AGACAAAGGA CAGAAGGGTG AGCCTGGAAA CTTTGGTGCC
    CTAGGACAGT CTGGACTAAG AGGTAAAGAT GGTGAACCAG GACTAAATGG GGACAAAGGC
    GAGAAGGGAG ACGCAGGTGT CCCCGGAGAA CCAGGGGAAA AGGGACAAAG GGGCTCTGTT
    GGACTGCCTG GCCGGATTGG TGATACTGGA ATAAAAGGAG ATATGGGGCC ACCTGGAAGA
    GATGGCATAG ATAGTAAAAA AGGTGATAAA GGAGAAGCCG GGCAGCCAGG AACACCTGGG
    GCAATGGTGA CTCAAATAGA CAAAAGTGTG CTTGTCAAAG GAGAAAAGGG AGAACCCGGA
    GAGGTGGGAG ATCCAGGAGA ATCGGGTTTT AAAGGAGAAA AAGGAGACAC GGGGCTACCA
    GGGCCCAGAG GACCTGAAGG CCGTCCAGGA CCACCTGGTG CTTCACCGTT GGAGTCCGCT
    TCTGAGAAAG GAATGAAAGG GGAAAAGGGA AGCCCAGGTT CTTCTGGAAA AAATGGCCTT
    GATGGAAAGC CTGGGAATCC TGGGTTACTT GGACAAAAGG GAGAAGCAGG CCAAAAAGGA
    GAACCAGGAA ATGTAGGCCT AACTGTAAGT GAAAAGGGAA ATAAAGGAGA AATAGGAAAC
    CAAGGCCCAC CAGGACCTCA GGGACCTCCT TGCACAGATT TAGAAATGAA GGATTTCGAA
    AGGCTATTTG AATCTTATGG CATTAAGCTG TCTCTACTGA AGGAACTGAC TGATCATATT
    ATACAAAATG GATTAGCTGA TGTAATTCAG CAACTAACAA ATTCCAAGAA AGACCGAACT
    TCTAAGAAGA AGCAAGTTTA TAAACAGTCA CCAGAATTCA CTGATGCCTT AAAACAAGAT
    CATATTAATG AGGCAGAAAA AAACCATGAG GAAGAAGAAG ATCCAAGCTT GCAGGCTGAG
    ATAATACCTA CAGTGGGGGT GACTGAGTCC CATACTCTAA CTCAGGACTT AAAAGAAGGC
    AATAAAGAAC AAAAGAAAAA GAAGAAAACA CATGTAGAGG CTGTTCCTAT TAACATTTAC
    ACAAATGTGA TCACTGAGGA ACAGGAGGAA ATCAGCCAAG ACCAGAGCCA CCATTTAACA
    ACCAATACTA CTTTTTTGCC AACTCAGCAA TCAATAGATT TGGAAGGATC TGATGCAAAT
    GCTGATAGAG TTGTCCAAGC ACAGATTGAC AAACATCAGA GGAAGCAAGA GAAAGAGGTT
    AGAAAGGAAA GGAAAAAAAA TCGCCTTGAA ACTACAGCTA CTGAAACAGA AATGAAGAAC
    CTTGTTTTGG AGGAAATTAC AGCAGTGACT GCTTATAAAG AAGAAAATTC ACAATCTGCC
    ACCACCCTGC CTGCAATAAT TAATGCAAAG AGGTTAACAT CTGACTTGAT AGAGGAGACA
    GACACAATCG TTCAGCCCAC ATCTGATACT ACTATACTAG TCGAAAAGCA TCAGGAAAAG
    GCAGTGAAAG AGAGCAAGAA GGACCAGAAA AAGAAAATGC GTACTGATCA TAAAGTCAAA
    GAACTCAAAA TACAGGGAAC TGCACAACAA GAGTTAAATC AGATACTTTC TGAGAGAACA
    AGCAGGGATC CCCACAGTGT TGATTCAAAT GCAGCAGCAA GCACTGAGCA TAGTTTGGAG
    AATCAGCAGG TCACCGGAAA TATATCCTTC AGGGTCAGCC TAAAAGACAA TGACTTGACA
    ATGATTGATG AGACGTCTTG GAATGTCACA GTAGTTGAAA CTGATAAAGC AGATCAAACT
    GCATCACAGG AGGATGAAGA ATACTTGGAG GTGCCAGAAA CTGAGCAGAC CATCACCAGT
    TCCATTAACT CATCAGAACA TATGGTACAC AATAGAATCA GGAGGATGGC AAAAGATTTA
    GAAAGGATAT TCGCCCCAGA GAAACTAAGA AGACAAAGGA GAACAAAGGA AGGAAAGGGG
    CGTTCAGGAT CTCAAAGAGA CAAAGGACAA AAGGGTGAAC CGGGGGAGGC TGGTGAGGCT
    GGGATTCCTG GTCTGAAGGG TGAAAAGGGA GATATTGGAG AACAGGGACA AAAGGGGGAA
    CCTGGTGTTG GACACCGTGG GCCAATTGGA CAGGCTGGAC CACCAGGTGT TAAGGGTGAG
    CCAGGAGAGA CGGGACCCCC AGGAGCTCAA GGAATTCAAG GTATCCAAGG CAACCCAGGC
    ATCTCAGGAT TCCAAGGTGA AAGAGGAGAA ACAGGATTTT CAGGGCCTCC TGGACCACCT
    GGAGAGAGAG GCAAGAAGGG AAAAAATGGA AATGCTGGTC AACCTGGGCC CCATGGATCA
    ACAGGAAAAC AGGGGAAACC AGGACTTCAA GGTAGTAAGG GAGAAAAGGG TGAAGCTGTG
    GTTGGTGAAC CTGGTCCTCG TGGGCCTCGC GGGCTTCCTG GCTTTAGGGG AGAAAATGGG
    ATCCCAGGCT TACCTGGACC TGCGGGAATT GTAGGTTTAA AGGGATTCCC AGGAATAAAG
    GGTGAACGAG GTGATCGGGG GATTTTTGGG ATTAAGGGAG AAAAGGGTGA ATCCACAACT
    CTCTTTGGCT CTCAAGGGTA TAAAGGAAAC AAAGGAGATC CGGGTGACAG AGGCTACCCA
    GGCTTTGATG GAGACAAAGG CGAGAAAGGA GAAGATGGAC CTGCTGGGGA AAAAGGTGTG
    AAAGGTGAAG CTGGTTCCAA AGGATCCATG GGACTATTTG GAGCTCGGGG TCCTGTTGGA
    CAGAAGGGTG AGCCAGGGGA GCATGGACTT CCAGGAAGAG CAGGAACAGC AGGACTTGAT
    GGAAAAAATG GACTCAAAGG AACAAAAGGT GACCGGGGTC TACAAGGACA AAGGGGAAAC
    CCGGGAGAGA AAGGTGATTC TGGATTGTCA GGAGATCCTG GAAAGAAAGG AGATAAGGGT
    CTGCGGGGAA TTCCAGGAAA AATGGGAGAG CCTGGGACAG AAGGTGAAAA AGGTGACCCT
    GGGGCTCAAG GAAATCCCGG TACAGGATCA GAAGGTCCTT CTGGACCTAA GGGTGAGAAA
    GGTGATAAAG GTGAAGATGG ACCCCCTGGA ATTGCTGGAG ATAAAGGATT TAAGGGTACT
    GATGGAGTGC GTGGACTGAA TGGCTTTAGG GGACCAATAG GCCATCCAGG AAAAGATGGC
    CCTCCAGGTC CTACTGGACC TTCAGGACAG AAAGGAGAGC CAGGGCCAAA GGGTGAAAGA
    GGAAAAAGGG GAAAATCCTT GCAGTGTCCA AGAGGAACCC CAGGGGATCC AGGAAACAAA
    GGAGAAACGG GAGTGGAGGG GCCTGAAGGT CTCAGAGGGG ACAAGGGAGA TCCAGGGCTC
    ACAGAGGAAG AAGTGAAAGA TTTGGTCAGA AAAGAAATGA GCGACAAATG TGCTTGTGCA
    GGAAAGGATT TTCATCTAGT AATTAAATCA TCACTCCCAG ATGAAGAGTA TGAAGATCCT
    GACCTAAATG AAAGTCCAGT GACTGCTCCA CCACAGGATA TTGTGACTGA AAATGATGTA
    GTAGTAGAAG CGTATGGAAA TGTAACACTT AATAGCACTT TAACTCCAGA TACGGAAGTG
    CAACAAAACG ACACAGACGG AAGTGATAAG GAGCTAATGG GACAATTGCA GAGAAGAAAA
    CGTGAAGCAG AAAATCTGTT TCGTAATCCC TGTTTGCTTC CAATGGATGA AGGATTCTGC
    TCCGATTACA CCTTGGTTTG GTATTACCAC CGCAAAACAG ACGTATGCCG GCCTTTTTTA
    TTTGGTGGTT GTGGGGGAAA TATCAATCGC TTTAAAACAA AAGACAGATG TGAGCAGAGG
    TGTAAAACTC GATCAGTATC ATCCCGAGGG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54926
    Clone ID Related Accession (Same CDS sequence) XM_031899078.1
    Accession Version XM_031899078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1141
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    1321
    1381
    1441
    1501
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    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGAC CATCACCAGT
    TCCATTAACT CATCAGAACA TATGGTACAC AATAGAATCA GGAGGATGGC AAAAGATTTA
    GAAAGGATAT TCGCCCCAGA GAAACTAAGA AGACAAAGGA GAACAAAGGA AGGAAAGGGG
    CGTTCAGGAT CTCAAAGAGA CAAAGGACAA AAGGGTGAAC CGGGGGAGGC TGGTGAGGCT
    GGGATTCCTG GTCTGAAGGG TGAAAAGGGA GATATTGGAG AACAGGGACA AAAGGGGGAA
    CCTGGTGTTG GACACCGTGG GCCAATTGGA CAGGCTGGAC CACCAGGTGT TAAGGGTGAG
    CCAGGAGAGA CGGGACCCCC AGGAGCTCAA GGAATTCAAG GTATCCAAGG CAACCCAGGC
    ATCTCAGGAT TCCAAGGTGA AAGAGGAGAA ACAGGATTTT CAGGGCCTCC TGGACCACCT
    GGAGAGAGAG GCAAGAAGGG AAAAAATGGA AATGCTGGTC AACCTGGGCC CCATGGATCA
    ACAGGAAAAC AGGGGAAACC AGGACTTCAA GGTAGTAAGG GAGAAAAGGG TGAAGCTGTG
    GTTGGTGAAC CTGGTCCTCG TGGGCCTCGC GGGCTTCCTG GCTTTAGGGG AGAAAATGGG
    ATCCCAGGCT TACCTGGACC TGCGGGAATT GTAGGTTTAA AGGGATTCCC AGGAATAAAG
    GGTGAACGAG GTGATCGGGG GATTTTTGGG ATTAAGGGAG AAAAGGGTGA ATCCACAACT
    CTCTTTGGCT CTCAAGGGTA TAAAGGAAAC AAAGGAGATC CGGGTGACAG AGGCTACCCA
    GGCTTTGATG GAGACAAAGG CGAGAAAGGA GAAGATGGAC CTGCTGGGGA AAAAGGTGTG
    AAAGGTGAAG CTGGTTCCAA AGGATCCATG GGACTATTTG GAGCTCGGGG TCCTGTTGGA
    CAGAAGGGTG AGCCAGGGGA GCATGGACTT CCAGGAAGAG CAGGAACAGC AGGACTTGAT
    GGAAAAAATG GACTCAAAGG AACAAAAGGT GACCGGGGTC TACAAGGACA AAGGGGAAAC
    CCGGGAGAGA AAGGTGATTC TGGATTGTCA GGAGATCCTG GAAAGAAAGG AGATAAGGGT
    CTGCGGGGAA TTCCAGGAAA AATGGGAGAG CCTGGGACAG AAGGTGAAAA AGGTGACCCT
    GGGGCTCAAG GAAATCCCGG TACAGGATCA GAAGGTCCTT CTGGACCTAA GGGTGAGAAA
    GGTGATAAAG GTGAAGATGG ACCCCCTGGA ATTGCTGGAG ATAAAGGATT TAAGGGTACT
    GATGGAGTGC GTGGACTGAA TGGCTTTAGG GGACCAATAG GCCATCCAGG AAAAGATGGC
    CCTCCAGGTC CTACTGGACC TTCAGGACAG AAAGGAGAGC CAGGGCCAAA GGGTGAAAGA
    GGAAAAAGGG GAAAATCCTT GCAGTGTCCA AGAGGAACCC CAGGGGATCC AGGAAACAAA
    GGAGAAACGG GAGTGGAGGG GCCTGAAGGT CTCAGAGGGG ACAAGGGAGA TCCAGGGCTC
    ACAGAGGAAG AAGTGAAAGA TTTGGTCAGA AAAGAAATGA GCGACAAATG TGCTTGTGCA
    GGAAAGGATT TTCATCTAGT AATTAAATCA TCACTCCCAG ATGAAGAGTA TGAAGATCCT
    GACCTAAATG AAAGTCCAGT GACTGCTCCA CCACAGGATA TTGTGACTGA AAATGATGTA
    GTAGTAGAAG CGTATGGAAA TGTAACACTT AATAGCACTT TAACTCCAGA TACGGAAGTG
    CAACAAAACG ACACAGACGG AAGTGATAAG GAGCTAATGG GACAATTGCA GAGAAGAAAA
    CGTGAAGCAG AAAATCTGTT TCGTAATCCC TGTTTGCTTC CAATGGATGA AGGATTCTGC
    TCCGATTACA CCTTGGTTTG GTATTACCAC CGCAAAACAG ACGTATGCCG GCCTTTTTTA
    TTTGGTGGTT GTGGGGGAAA TATCAATCGC TTTAAAACAA AAGACAGATG TGAGCAGAGG
    TGTAAAACTC GATCAGTATC ATCCCGAGGG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754938.1
    CDS1388..9823
    Translation

    Target ORF information:

    RefSeq Version XM_031899078.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGAC CATCACCAGT
    TCCATTAACT CATCAGAACA TATGGTACAC AATAGAATCA GGAGGATGGC AAAAGATTTA
    GAAAGGATAT TCGCCCCAGA GAAACTAAGA AGACAAAGGA GAACAAAGGA AGGAAAGGGG
    CGTTCAGGAT CTCAAAGAGA CAAAGGACAA AAGGGTGAAC CGGGGGAGGC TGGTGAGGCT
    GGGATTCCTG GTCTGAAGGG TGAAAAGGGA GATATTGGAG AACAGGGACA AAAGGGGGAA
    CCTGGTGTTG GACACCGTGG GCCAATTGGA CAGGCTGGAC CACCAGGTGT TAAGGGTGAG
    CCAGGAGAGA CGGGACCCCC AGGAGCTCAA GGAATTCAAG GTATCCAAGG CAACCCAGGC
    ATCTCAGGAT TCCAAGGTGA AAGAGGAGAA ACAGGATTTT CAGGGCCTCC TGGACCACCT
    GGAGAGAGAG GCAAGAAGGG AAAAAATGGA AATGCTGGTC AACCTGGGCC CCATGGATCA
    ACAGGAAAAC AGGGGAAACC AGGACTTCAA GGTAGTAAGG GAGAAAAGGG TGAAGCTGTG
    GTTGGTGAAC CTGGTCCTCG TGGGCCTCGC GGGCTTCCTG GCTTTAGGGG AGAAAATGGG
    ATCCCAGGCT TACCTGGACC TGCGGGAATT GTAGGTTTAA AGGGATTCCC AGGAATAAAG
    GGTGAACGAG GTGATCGGGG GATTTTTGGG ATTAAGGGAG AAAAGGGTGA ATCCACAACT
    CTCTTTGGCT CTCAAGGGTA TAAAGGAAAC AAAGGAGATC CGGGTGACAG AGGCTACCCA
    GGCTTTGATG GAGACAAAGG CGAGAAAGGA GAAGATGGAC CTGCTGGGGA AAAAGGTGTG
    AAAGGTGAAG CTGGTTCCAA AGGATCCATG GGACTATTTG GAGCTCGGGG TCCTGTTGGA
    CAGAAGGGTG AGCCAGGGGA GCATGGACTT CCAGGAAGAG CAGGAACAGC AGGACTTGAT
    GGAAAAAATG GACTCAAAGG AACAAAAGGT GACCGGGGTC TACAAGGACA AAGGGGAAAC
    CCGGGAGAGA AAGGTGATTC TGGATTGTCA GGAGATCCTG GAAAGAAAGG AGATAAGGGT
    CTGCGGGGAA TTCCAGGAAA AATGGGAGAG CCTGGGACAG AAGGTGAAAA AGGTGACCCT
    GGGGCTCAAG GAAATCCCGG TACAGGATCA GAAGGTCCTT CTGGACCTAA GGGTGAGAAA
    GGTGATAAAG GTGAAGATGG ACCCCCTGGA ATTGCTGGAG ATAAAGGATT TAAGGGTACT
    GATGGAGTGC GTGGACTGAA TGGCTTTAGG GGACCAATAG GCCATCCAGG AAAAGATGGC
    CCTCCAGGTC CTACTGGACC TTCAGGACAG AAAGGAGAGC CAGGGCCAAA GGGTGAAAGA
    GGAAAAAGGG GAAAATCCTT GCAGTGTCCA AGAGGAACCC CAGGGGATCC AGGAAACAAA
    GGAGAAACGG GAGTGGAGGG GCCTGAAGGT CTCAGAGGGG ACAAGGGAGA TCCAGGGCTC
    ACAGAGGAAG AAGTGAAAGA TTTGGTCAGA AAAGAAATGA GCGACAAATG TGCTTGTGCA
    GGAAAGGATT TTCATCTAGT AATTAAATCA TCACTCCCAG ATGAAGAGTA TGAAGATCCT
    GACCTAAATG AAAGTCCAGT GACTGCTCCA CCACAGGATA TTGTGACTGA AAATGATGTA
    GTAGTAGAAG CGTATGGAAA TGTAACACTT AATAGCACTT TAACTCCAGA TACGGAAGTG
    CAACAAAACG ACACAGACGG AAGTGATAAG GAGCTAATGG GACAATTGCA GAGAAGAAAA
    CGTGAAGCAG AAAATCTGTT TCGTAATCCC TGTTTGCTTC CAATGGATGA AGGATTCTGC
    TCCGATTACA CCTTGGTTTG GTATTACCAC CGCAAAACAG ACGTATGCCG GCCTTTTTTA
    TTTGGTGGTT GTGGGGGAAA TATCAATCGC TTTAAAACAA AAGACAGATG TGAGCAGAGG
    TGTAAAACTC GATCAGTATC ATCCCGAGGG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54927
    Clone ID Related Accession (Same CDS sequence) XM_031899079.1
    Accession Version XM_031899079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGTAGGTT TAAAGGGATT CCCAGGAATA
    AAGGGTGAAC GAGGTGATCG GGGGATTTTT GGGATTAAGG GAGAAAAGGG TGAATCCACA
    ACTCTCTTTG GCTCTCAAGG GTATAAAGGA AACAAAGGAG ATCCGGGTGA CAGAGGCTAC
    CCAGGCTTTG ATGGAGACAA AGGCGAGAAA GGAGAAGATG GACCTGCTGG GGAAAAAGGT
    GTGAAAGGTG AAGCTGGTTC CAAAGGATCC ATGGGACTAT TTGGAGCTCG GGGTCCTGTT
    GGACAGAAGG GTGAGCCAGG GGAGCATGGA CTTCCAGGAA GAGCAGGAAC AGCAGGACTT
    GATGGAAAAA ATGGACTCAA AGGAACAAAA GGTGACCGGG GTCTACAAGG ACAAAGGGGA
    AACCCGGGAG AGAAAGGTGA TTCTGGATTG TCAGGAGATC CTGGAAAGAA AGGAGATAAG
    GGTCTGCGGG GAATTCCAGG AAAAATGGGA GAGCCTGGGA CAGAAGGTGA AAAAGGTGAC
    CCTGGGGCTC AAGGAAATCC CGGTACAGGA TCAGAAGGTC CTTCTGGACC TAAGGGTGAG
    AAAGGTGATA AAGGTGAAGA TGGACCCCCT GGAATTGCTG GAGATAAAGG ATTTAAGGGT
    ACTGATGGAG TGCGTGGACT GAATGGCTTT AGGGGACCAA TAGGCCATCC AGGAAAAGAT
    GGCCCTCCAG GTCCTACTGG ACCTTCAGGA CAGAAAGGAG AGCCAGGGCC AAAGGGTGAA
    AGAGGAAAAA GGGGAAAATC CTTGCAGTGT CCAAGAGGAA CCCCAGGGGA TCCAGGAAAC
    AAAGGAGAAA CGGGAGTGGA GGGGCCTGAA GGTCTCAGAG GGGACAAGGG AGATCCAGGG
    CTCACAGAGG AAGAAGTGAA AGATTTGGTC AGAAAAGAAA TGAGCGACAA ATGTGGAAAG
    GATTTTCATC TAGTAATTAA ATCATCACTC CCAGATGAAG AGTATGAAGA TCCTGACCTA
    AATGAAAGTC CAGTGACTGC TCCACCACAG GATATTGTGA CTGAAAATGA TGTAGTAGTA
    GAAGCGTATG GAAATGTAAC ACTTAATAGC ACTTTAACTC CAGATACGGA AGTGCAACAA
    AACGACACAG ACGGAAGTGA TAAGGAGCTA ATGGGACAAT TGCAGAGAAG AAAACGTGAA
    GCAGAAAATC TGTTTCGTAA TCCCTGTTTG CTTCCAATGG ATGAAGGATT CTGCTCCGAT
    TACACCTTGG TTTGGTATTA CCACCGCAAA ACAGACGTAT GCCGGCCTTT TTTATTTGGT
    GGTTGTGGGG GAAATATCAA TCGCTTTAAA ACAAAAGACA GATGTGAGCA GAGGTGTAAA
    ACTCGATCAG TATCATCCCG AGGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754939.1
    CDS1388..9817
    Translation

    Target ORF information:

    RefSeq Version XM_031899079.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGTAGGTT TAAAGGGATT CCCAGGAATA
    AAGGGTGAAC GAGGTGATCG GGGGATTTTT GGGATTAAGG GAGAAAAGGG TGAATCCACA
    ACTCTCTTTG GCTCTCAAGG GTATAAAGGA AACAAAGGAG ATCCGGGTGA CAGAGGCTAC
    CCAGGCTTTG ATGGAGACAA AGGCGAGAAA GGAGAAGATG GACCTGCTGG GGAAAAAGGT
    GTGAAAGGTG AAGCTGGTTC CAAAGGATCC ATGGGACTAT TTGGAGCTCG GGGTCCTGTT
    GGACAGAAGG GTGAGCCAGG GGAGCATGGA CTTCCAGGAA GAGCAGGAAC AGCAGGACTT
    GATGGAAAAA ATGGACTCAA AGGAACAAAA GGTGACCGGG GTCTACAAGG ACAAAGGGGA
    AACCCGGGAG AGAAAGGTGA TTCTGGATTG TCAGGAGATC CTGGAAAGAA AGGAGATAAG
    GGTCTGCGGG GAATTCCAGG AAAAATGGGA GAGCCTGGGA CAGAAGGTGA AAAAGGTGAC
    CCTGGGGCTC AAGGAAATCC CGGTACAGGA TCAGAAGGTC CTTCTGGACC TAAGGGTGAG
    AAAGGTGATA AAGGTGAAGA TGGACCCCCT GGAATTGCTG GAGATAAAGG ATTTAAGGGT
    ACTGATGGAG TGCGTGGACT GAATGGCTTT AGGGGACCAA TAGGCCATCC AGGAAAAGAT
    GGCCCTCCAG GTCCTACTGG ACCTTCAGGA CAGAAAGGAG AGCCAGGGCC AAAGGGTGAA
    AGAGGAAAAA GGGGAAAATC CTTGCAGTGT CCAAGAGGAA CCCCAGGGGA TCCAGGAAAC
    AAAGGAGAAA CGGGAGTGGA GGGGCCTGAA GGTCTCAGAG GGGACAAGGG AGATCCAGGG
    CTCACAGAGG AAGAAGTGAA AGATTTGGTC AGAAAAGAAA TGAGCGACAA ATGTGGAAAG
    GATTTTCATC TAGTAATTAA ATCATCACTC CCAGATGAAG AGTATGAAGA TCCTGACCTA
    AATGAAAGTC CAGTGACTGC TCCACCACAG GATATTGTGA CTGAAAATGA TGTAGTAGTA
    GAAGCGTATG GAAATGTAAC ACTTAATAGC ACTTTAACTC CAGATACGGA AGTGCAACAA
    AACGACACAG ACGGAAGTGA TAAGGAGCTA ATGGGACAAT TGCAGAGAAG AAAACGTGAA
    GCAGAAAATC TGTTTCGTAA TCCCTGTTTG CTTCCAATGG ATGAAGGATT CTGCTCCGAT
    TACACCTTGG TTTGGTATTA CCACCGCAAA ACAGACGTAT GCCGGCCTTT TTTATTTGGT
    GGTTGTGGGG GAAATATCAA TCGCTTTAAA ACAAAAGACA GATGTGAGCA GAGGTGTAAA
    ACTCGATCAG TATCATCCCG AGGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54928
    Clone ID Related Accession (Same CDS sequence) XM_031899080.1
    Accession Version XM_031899080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GACACTCCTA AAAGTCATTA TCTGGCGTCC GGTGTCAGGT CCTATCACAT CACAAATTTG
    TACCCAAACA CAAAGTACAC TGTGAATGTT CAGGCAGTAT ATAATAACTT TGAAGGGCCG
    CCAAGCACTC TAATTCAAAC CACAGACTCA AATCCTGTGC AGCTACTGCC TGTGAAAGAT
    CTGCAGATCA TTGATACTGG AGTCAATATG CTGAAACTGT CTTGGAAGAA AACACCTGGA
    ATAACACATT ATAAGATCTC ATGGACACCA TTTGATGGTG GTGCAAGAAA CTCTAAGATT
    GTCTCTGCTG GTACGTCATT TTTCACAATC CCTGACCTTA AGGAGAGTTC CACTTACAGC
    ATCTATGTAT CCTCGATAAT CGGGAATCGG GAAGGAAGTT CAGTGGTTCT TACTGCAAAA
    ACATTGGATT TGCCAAAAGT AGAATCTTTT GAAGTCAAAG AGACCACAGA GGACAGTGTT
    TTATTAAGCT GGACTGGAGT CACTGGAGCA TCTTCATATA TTTTAACATG GAGGATGGCT
    TCAGACCCAG AATCCTCCGT GGAGCAGTTA GCTCCAACAT ATAGATCTTT CCGGCTCATG
    AATTTGGAGA ATGGAAAGTC ATATATTTTT GCAATAAAGC CTCTTTTTGG AGAGATGGAA
    GGGCCTGAGA GAACAATCAC ACACACAATC GTTGGACCCC CCAAAAAACA GATCTACCCT
    TCCCCTACCC TTCAAATACC TGTTCCAACA CCTCAAAGGA CCCTAACAAC ACAAGTTATC
    AACCACCCTC CCATAGTCAC CACAACTGAA GCTGCAAAAT CCACAAGCAC TGTCTCACAT
    GGTTCTTCCA CAACTACTTC TGAACCCATT TGTGCAAAAC TGAAAGCTGA CATAGTATTT
    CTGGTTGATG AGTCCTCCAG CATTGGCCCA TCCAATTTTA TTAAGGTTAA GGATTTCCTC
    TACAGAATTG TCTCTTACTT TCCCAAAATT GGACCTCAAG GAACACAGAT TGCTATTGTT
    CAGTACAGCG AAGAACCCAG AACAGAATTC CACTTGAATG AGTATAAGGA GAGAAATTCT
    GTTTTGAAAG CAATCAGAGC TCTACAGTAT TATGGAGGCA ACACCAAGAC TGGTCGTGGT
    ATAGGACATG CACTGAAGGA AATGTTTCAG GTTTCCAAAG GGATGAGACC ATCTGCTCCA
    CATATACTTG TGCTAATAAC TGACGGAAGG TCTCAGGATA ATGTAATACA GCCAGCCAAA
    GTAGCTCATG TATTAGGCAT TCGTATGATT GCAGTCGGTG TGGCTGGGGC AGATATGGAT
    GAACTGAAAG GAATGATGAT GAACCGTAAT TTAGAAAACA TCTTCTTTGC CAGTACATTT
    GATGATTTTC CTCTAATTGT ACAAGATCTG ATAGATGCTA TTTGCACTGA AACCGAACAT
    GCAGTTAAAG AAGAACCTAC TAAGTTCAGA GCACCAGATA AAGAAGAGGA GCCAGAAAGA
    CCAAAACCTC CTCCTGGGAA TCGAGAGGGA CCCTGTGGAA ATACCATGTG CCCAACGGGG
    CAAAAGGGAC AAAAGGGAGA AAAGGGTCCA CCGGGAGATT CAGTTTTCAC AGGTTTACAG
    ACTGGTGGCT TTGACCCTTT TGATTTGAAT TCAAAAGGAG AAAAAGGAGA GAGAGGTCTT
    CCTGGACAAG ATGGTGTTCC TGGCCTTCCA GGACGTCCTG GTCGAACTGG GCCCCCAGGG
    CCACCTGGCC TTATGGGACA AAAAGGATTT CAAGGCGATA TTGGCCCCCA AGGATATCCA
    GGTCAGCCAG GTCCCAAAGG AGACAGGGGA GAACCAGGCT ATATACTAGG AGGAGTTGAT
    GGAATGGCTG GTCTAAGTGC AATACCTGGG TCTCCTGGAC CCAAAGGACA GCCTGGGGTT
    CCAGGGCTAC CAGGACCACC TGGACTTCCA GGATTTCCTG GACCTCAGGG ACCTCCAGGA
    ACTTCTATAA AGGGGGAACC TGGTGAAACA GGGAGCCAGG GTCCTCGTGG AAAAAGTGGA
    CCAAAAGGAG ATAAAGGAGA CCAGGGGGAT GCAGGAAGAG CTGGCTTACC TGGCCCAATT
    GGGCTTGATG GCTCTCCAGG AATTCATGGA ATTAAAGGAG ACAAGGGGGA GGCTGGTATT
    GGAATCCCTG GATTACCTGG TTTAAAGGGT ATAACAGGTG AAAAGGGTGT CATTGGACTT
    CCTGGCCCTC AAGGACAGAA GGGTACTCAA GGAATACAAG GTGTAGAAGG TGTCGCAGGT
    TTAAGGGGTA AAAAAGGTCA AGATGGTGAA AAGGGTGAAA AGGGAGAACA TGGTGCTATG
    GGAATGACTG GTCCTCCTGG AATAGCTGGT CTCTCTGGAC CAATGGGGCA AAAGGGGGAT
    CAGGGGTTGC AAGGTTTTCC TGGCGCTCCT GCTATGGGGG TAATTGGGCC TTCTGGTAAA
    AAAGGTGCCA GAGGTGATAT TGGACCAGTT GGGCCAATTG GACCTAAAGG CATTATGGGA
    AGTCAAGGAG ATAAAGGAAT TAAGGGAGAA CCAGGATATG GTATACCTGG GCAGCCTGGA
    TTAAAGGGAG ATCCAGGTGA CAGAGGAAAC ATAGGATTAT CTGGAAAACC AGGTCCCAAA
    GGAGATTTTG GAGACAAAGG ACAGAAGGGT GAGCCTGGAA ACTTTGGTGC CCTAGGACAG
    TCTGGACTAA GAGGTAAAGA TGGTGAACCA GGACTAAATG GGGACAAAGG CGAGAAGGGA
    GACGCAGGTG TCCCCGGAGA ACCAGGGGAA AAGGGACAAA GGGGCTCTGT TGGACTGCCT
    GGCCGGATTG GTGATACTGG AATAAAAGGA GATATGGGGC CACCTGGAAG AGATGGCATA
    GATAGTAAAA AAGGTGATAA AGGAGAAGCC GGGCAGCCAG GAACACCTGG GGCAATGGTG
    ACTCAAATAG ACAAAAGTGT GCTTGTCAAA GGAGAAAAGG GAGAACCCGG AGAGGTGGGA
    GATCCAGGAG AATCGGGTTT TAAAGGAGAA AAAGGAGACA CGGGGCTACC AGGGCCCAGA
    GGACCTGAAG GCCGTCCAGG ACCACCTGGT GCTTCACCGT TGGAGTCCGC TTCTGAGAAA
    GGAATGAAAG GGGAAAAGGG AAGCCCAGGT TCTTCTGGAA AAAATGGCCT TGATGGAAAG
    CCTGGGAATC CTGGGTTACT TGGACAAAAG GGAGAAGCAG GCCAAAAAGG AGAACCAGGA
    AATGTAGGCC TAACTGTAAG TGAAAAGGGA AATAAAGGAG AAATAGGAAA CCAAGGCCCA
    CCAGGACCTC AGGGACCTCC TTGCACAGAT TTAGAAATGA AGGATTTCGA AAGGCTATTT
    GAATCTTATG GCATTAAGCT GTCTCTACTG AAGGAACTGA CTGATCATAT TATACAAAAT
    GGATTAGCTG ATGTAATTCA GCAACTAACA AATTCCAAGA AAGACCGAAC TTCTAAGAAG
    AAGCAAGTTT ATAAACAGTC ACCAGAATTC ACTGATGCCT TAAAACAAGA TCATATTAAT
    GAGGCAGAAA AAAACCATGA GGAAGAAGAA GATCCAAGCT TGCAGGCTGA GATAATACCT
    ACAGTGGGGG TGACTGAGTC CCATACTCTA ACTCAGGACT TAAAAGAAGG CAATAAAGAA
    CAAAAGAAAA AGAAGAAAAC ACATGTAGAG GCTGTTCCTA TTAACATTTA CACAAATGTG
    ATCACTGAGG AACAGGAGGA AATCAGCCAA GACCAGAGCC ACCATTTAAC AACCAATACT
    ACTTTTTTGC CAACTCAGCA ATCAATAGAT TTGGAAGGAT CTGATGCAAA TGCTGATAGA
    GTTGTCCAAG CACAGATTGA CAAACATCAG AGGAAGCAAG AGAAAGAGGT TAGAAAGGAA
    AGGAAAAAAA ATCGCCTTGA AACTACAGCT ACTGAAACAG AAATGAAGAA CCTTGTTTTG
    GAGGAAATTA CAGCAGTGAC TGCTTATAAA GAAGAAAATT CACAATCTGC CACCACCCTG
    CCTGCAATAA TTAATGCAAA GAGGTTAACA TCTGACTTGA TAGAGGAGAC AGACACAATC
    GTTCAGCCCA CATCTGATAC TACTATACTA GTCGAAAAGC ATCAGGAAAA GGCAGTGAAA
    GAGAGCAAGA AGGACCAGAA AAAGAAAATG CGTACTGATC ATAAAGTCAA AGAACTCAAA
    ATACAGGGAA CTGCACAACA AGAGTTAAAT CAGATACTTT CTGAGAGAAC AAGCAGGGAT
    CCCCACAGTG TTGATTCAAA TGCAGCAGCA AGCACTGAGC ATAGTTTGGA GAATCAGCAG
    GTCACCGGAA ATATATCCTT CAGGGTCAGC CTAAAAGACA ATGACTTGAC AATGATTGAT
    GAGACGTCTT GGAATGTCAC AGTAGTTGAA ACTGATAAAG CAGATCAAAC TGCATCACAG
    GAGGATGAAG AATACTTGGA GGTGCCAGAA ACTGAGCAGA CCATCACCAG TTCCATTAAC
    TCATCAGAAC ATATGGTACA CAATAGAATC AGGAGGATGG CAAAAGATTT AGAAAGGATA
    TTCGCCCCAG AGAAACTAAG AAGACAAAGG AGAACAAAGG AAGGAAAGGG GCGTTCAGGA
    TCTCAAAGAG ACAAAGGACA AAAGGGTGAA CCGGGGGAGG CTGGTGAGGC TGGGATTCCT
    GGTCTGAAGG GTGAAAAGGG AGATATTGGA GAACAGGGAC AAAAGGGGGA ACCTGGTGTT
    GGACACCGTG GGCCAATTGG ACAGGCTGGA CCACCAGGTG TTAAGGGTGA GCCAGGAGAG
    ACGGGACCCC CAGGAGCTCA AGGAATTCAA GGTATCCAAG GCAACCCAGG CATCTCAGGA
    TTCCAAGGTG AAAGAGGAGA AACAGGATTT TCAGGGCCTC CTGGACCACC TGGAGAGAGA
    GGCAAGAAGG GAAAAAATGG AAATGCTGGT CAACCTGGGC CCCATGGATC AACAGGAAAA
    CAGGGGAAAC CAGGACTTCA AGGTAGTAAG GGAGAAAAGG GTGAAGCTGT GGTTGGTGAA
    CCTGGTCCTC GTGGGCCTCG CGGGCTTCCT GGCTTTAGGG GAGAAAATGG GATCCCAGGC
    TTACCTGGAC CTGCGGGAAT TGTAGGTTTA AAGGGATTCC CAGGAATAAA GGGTGAACGA
    GGTGATCGGG GGATTTTTGG GATTAAGGGA GAAAAGGGTG AATCCACAAC TCTCTTTGGC
    TCTCAAGGGT ATAAAGGAAA CAAAGGAGAT CCGGGTGACA GAGGCTACCC AGGCTTTGAT
    GGAGACAAAG GCGAGAAAGG AGAAGATGGA CCTGCTGGGG AAAAAGGTGT GAAAGGTGAA
    GCTGGTTCCA AAGGATCCAT GGGACTATTT GGAGCTCGGG GTCCTGTTGG ACAGAAGGGT
    GAGCCAGGGG AGCATGGACT TCCAGGAAGA GCAGGAACAG CAGGACTTGA TGGAAAAAAT
    GGACTCAAAG GAACAAAAGG TGACCGGGGT CTACAAGGAC AAAGGGGAAA CCCGGGAGAG
    AAAGGTGATT CTGGATTGTC AGGAGATCCT GGAAAGAAAG GAGATAAGGG TCTGCGGGGA
    ATTCCAGGAA AAATGGGAGA GCCTGGGACA GAAGGTGAAA AAGGTGACCC TGGGGCTCAA
    GGAAATCCCG GTACAGGATC AGAAGGTCCT TCTGGACCTA AGGGTGAGAA AGGTGATAAA
    GGTGAAGATG GACCCCCTGG AATTGCTGGA GATAAAGGAT TTAAGGGTAC TGATGGAGTG
    CGTGGACTGA ATGGCTTTAG GGGACCAATA GGCCATCCAG GAAAAGATGG CCCTCCAGGT
    CCTACTGGAC CTTCAGGACA GAAAGGAGAG CCAGGGCCAA AGGGTGAAAG AGGAAAAAGG
    GGAAAATCCT TGCAGTGTCC AAGAGGAACC CCAGGGGATC CAGGAAACAA AGGAGAAACG
    GGAGTGGAGG GGCCTGAAGG TCTCAGAGGG GACAAGGGAG ATCCAGGGCT CACAGAGGAA
    GAAGTGAAAG ATTTGGTCAG AAAAGAAATG AGCGACAAAT GTGCTTGTGC AGGAAAGGAT
    TTTCATCTAG TAATTAAATC ATCACTCCCA GATGAAGAGT ATGAAGATCC TGACCTAAAT
    GAAAGTCCAG TGACTGCTCC ACCACAGGAT ATTGTGACTG AAAATGATGT AGTAGTAGAA
    GCGTATGGAA ATGTAACACT TAATAGCACT TTAACTCCAG ATACGGAAGT GCAACAAAAC
    GACACAGACG GAAGTGATAA GGAGCTAATG GGACAATTGC AGAGAAGAAA ACGTGAAGCA
    GAAAATCTGT TTCGTAATCC CTGTTTGCTT CCAATGGATG AAGGATTCTG CTCCGATTAC
    ACCTTGGTTT GGTATTACCA CCGCAAAACA GACGTATGCC GGCCTTTTTT ATTTGGTGGT
    TGTGGGGGAA ATATCAATCG CTTTAAAACA AAAGACAGAT GTGAGCAGAG GTGTAAAACT
    CGATCAGTAT CATCCCGAGG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754940.1
    CDS1387..9813
    Translation

    Target ORF information:

    RefSeq Version XM_031899080.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GACACTCCTA AAAGTCATTA TCTGGCGTCC GGTGTCAGGT CCTATCACAT CACAAATTTG
    TACCCAAACA CAAAGTACAC TGTGAATGTT CAGGCAGTAT ATAATAACTT TGAAGGGCCG
    CCAAGCACTC TAATTCAAAC CACAGACTCA AATCCTGTGC AGCTACTGCC TGTGAAAGAT
    CTGCAGATCA TTGATACTGG AGTCAATATG CTGAAACTGT CTTGGAAGAA AACACCTGGA
    ATAACACATT ATAAGATCTC ATGGACACCA TTTGATGGTG GTGCAAGAAA CTCTAAGATT
    GTCTCTGCTG GTACGTCATT TTTCACAATC CCTGACCTTA AGGAGAGTTC CACTTACAGC
    ATCTATGTAT CCTCGATAAT CGGGAATCGG GAAGGAAGTT CAGTGGTTCT TACTGCAAAA
    ACATTGGATT TGCCAAAAGT AGAATCTTTT GAAGTCAAAG AGACCACAGA GGACAGTGTT
    TTATTAAGCT GGACTGGAGT CACTGGAGCA TCTTCATATA TTTTAACATG GAGGATGGCT
    TCAGACCCAG AATCCTCCGT GGAGCAGTTA GCTCCAACAT ATAGATCTTT CCGGCTCATG
    AATTTGGAGA ATGGAAAGTC ATATATTTTT GCAATAAAGC CTCTTTTTGG AGAGATGGAA
    GGGCCTGAGA GAACAATCAC ACACACAATC GTTGGACCCC CCAAAAAACA GATCTACCCT
    TCCCCTACCC TTCAAATACC TGTTCCAACA CCTCAAAGGA CCCTAACAAC ACAAGTTATC
    AACCACCCTC CCATAGTCAC CACAACTGAA GCTGCAAAAT CCACAAGCAC TGTCTCACAT
    GGTTCTTCCA CAACTACTTC TGAACCCATT TGTGCAAAAC TGAAAGCTGA CATAGTATTT
    CTGGTTGATG AGTCCTCCAG CATTGGCCCA TCCAATTTTA TTAAGGTTAA GGATTTCCTC
    TACAGAATTG TCTCTTACTT TCCCAAAATT GGACCTCAAG GAACACAGAT TGCTATTGTT
    CAGTACAGCG AAGAACCCAG AACAGAATTC CACTTGAATG AGTATAAGGA GAGAAATTCT
    GTTTTGAAAG CAATCAGAGC TCTACAGTAT TATGGAGGCA ACACCAAGAC TGGTCGTGGT
    ATAGGACATG CACTGAAGGA AATGTTTCAG GTTTCCAAAG GGATGAGACC ATCTGCTCCA
    CATATACTTG TGCTAATAAC TGACGGAAGG TCTCAGGATA ATGTAATACA GCCAGCCAAA
    GTAGCTCATG TATTAGGCAT TCGTATGATT GCAGTCGGTG TGGCTGGGGC AGATATGGAT
    GAACTGAAAG GAATGATGAT GAACCGTAAT TTAGAAAACA TCTTCTTTGC CAGTACATTT
    GATGATTTTC CTCTAATTGT ACAAGATCTG ATAGATGCTA TTTGCACTGA AACCGAACAT
    GCAGTTAAAG AAGAACCTAC TAAGTTCAGA GCACCAGATA AAGAAGAGGA GCCAGAAAGA
    CCAAAACCTC CTCCTGGGAA TCGAGAGGGA CCCTGTGGAA ATACCATGTG CCCAACGGGG
    CAAAAGGGAC AAAAGGGAGA AAAGGGTCCA CCGGGAGATT CAGTTTTCAC AGGTTTACAG
    ACTGGTGGCT TTGACCCTTT TGATTTGAAT TCAAAAGGAG AAAAAGGAGA GAGAGGTCTT
    CCTGGACAAG ATGGTGTTCC TGGCCTTCCA GGACGTCCTG GTCGAACTGG GCCCCCAGGG
    CCACCTGGCC TTATGGGACA AAAAGGATTT CAAGGCGATA TTGGCCCCCA AGGATATCCA
    GGTCAGCCAG GTCCCAAAGG AGACAGGGGA GAACCAGGCT ATATACTAGG AGGAGTTGAT
    GGAATGGCTG GTCTAAGTGC AATACCTGGG TCTCCTGGAC CCAAAGGACA GCCTGGGGTT
    CCAGGGCTAC CAGGACCACC TGGACTTCCA GGATTTCCTG GACCTCAGGG ACCTCCAGGA
    ACTTCTATAA AGGGGGAACC TGGTGAAACA GGGAGCCAGG GTCCTCGTGG AAAAAGTGGA
    CCAAAAGGAG ATAAAGGAGA CCAGGGGGAT GCAGGAAGAG CTGGCTTACC TGGCCCAATT
    GGGCTTGATG GCTCTCCAGG AATTCATGGA ATTAAAGGAG ACAAGGGGGA GGCTGGTATT
    GGAATCCCTG GATTACCTGG TTTAAAGGGT ATAACAGGTG AAAAGGGTGT CATTGGACTT
    CCTGGCCCTC AAGGACAGAA GGGTACTCAA GGAATACAAG GTGTAGAAGG TGTCGCAGGT
    TTAAGGGGTA AAAAAGGTCA AGATGGTGAA AAGGGTGAAA AGGGAGAACA TGGTGCTATG
    GGAATGACTG GTCCTCCTGG AATAGCTGGT CTCTCTGGAC CAATGGGGCA AAAGGGGGAT
    CAGGGGTTGC AAGGTTTTCC TGGCGCTCCT GCTATGGGGG TAATTGGGCC TTCTGGTAAA
    AAAGGTGCCA GAGGTGATAT TGGACCAGTT GGGCCAATTG GACCTAAAGG CATTATGGGA
    AGTCAAGGAG ATAAAGGAAT TAAGGGAGAA CCAGGATATG GTATACCTGG GCAGCCTGGA
    TTAAAGGGAG ATCCAGGTGA CAGAGGAAAC ATAGGATTAT CTGGAAAACC AGGTCCCAAA
    GGAGATTTTG GAGACAAAGG ACAGAAGGGT GAGCCTGGAA ACTTTGGTGC CCTAGGACAG
    TCTGGACTAA GAGGTAAAGA TGGTGAACCA GGACTAAATG GGGACAAAGG CGAGAAGGGA
    GACGCAGGTG TCCCCGGAGA ACCAGGGGAA AAGGGACAAA GGGGCTCTGT TGGACTGCCT
    GGCCGGATTG GTGATACTGG AATAAAAGGA GATATGGGGC CACCTGGAAG AGATGGCATA
    GATAGTAAAA AAGGTGATAA AGGAGAAGCC GGGCAGCCAG GAACACCTGG GGCAATGGTG
    ACTCAAATAG ACAAAAGTGT GCTTGTCAAA GGAGAAAAGG GAGAACCCGG AGAGGTGGGA
    GATCCAGGAG AATCGGGTTT TAAAGGAGAA AAAGGAGACA CGGGGCTACC AGGGCCCAGA
    GGACCTGAAG GCCGTCCAGG ACCACCTGGT GCTTCACCGT TGGAGTCCGC TTCTGAGAAA
    GGAATGAAAG GGGAAAAGGG AAGCCCAGGT TCTTCTGGAA AAAATGGCCT TGATGGAAAG
    CCTGGGAATC CTGGGTTACT TGGACAAAAG GGAGAAGCAG GCCAAAAAGG AGAACCAGGA
    AATGTAGGCC TAACTGTAAG TGAAAAGGGA AATAAAGGAG AAATAGGAAA CCAAGGCCCA
    CCAGGACCTC AGGGACCTCC TTGCACAGAT TTAGAAATGA AGGATTTCGA AAGGCTATTT
    GAATCTTATG GCATTAAGCT GTCTCTACTG AAGGAACTGA CTGATCATAT TATACAAAAT
    GGATTAGCTG ATGTAATTCA GCAACTAACA AATTCCAAGA AAGACCGAAC TTCTAAGAAG
    AAGCAAGTTT ATAAACAGTC ACCAGAATTC ACTGATGCCT TAAAACAAGA TCATATTAAT
    GAGGCAGAAA AAAACCATGA GGAAGAAGAA GATCCAAGCT TGCAGGCTGA GATAATACCT
    ACAGTGGGGG TGACTGAGTC CCATACTCTA ACTCAGGACT TAAAAGAAGG CAATAAAGAA
    CAAAAGAAAA AGAAGAAAAC ACATGTAGAG GCTGTTCCTA TTAACATTTA CACAAATGTG
    ATCACTGAGG AACAGGAGGA AATCAGCCAA GACCAGAGCC ACCATTTAAC AACCAATACT
    ACTTTTTTGC CAACTCAGCA ATCAATAGAT TTGGAAGGAT CTGATGCAAA TGCTGATAGA
    GTTGTCCAAG CACAGATTGA CAAACATCAG AGGAAGCAAG AGAAAGAGGT TAGAAAGGAA
    AGGAAAAAAA ATCGCCTTGA AACTACAGCT ACTGAAACAG AAATGAAGAA CCTTGTTTTG
    GAGGAAATTA CAGCAGTGAC TGCTTATAAA GAAGAAAATT CACAATCTGC CACCACCCTG
    CCTGCAATAA TTAATGCAAA GAGGTTAACA TCTGACTTGA TAGAGGAGAC AGACACAATC
    GTTCAGCCCA CATCTGATAC TACTATACTA GTCGAAAAGC ATCAGGAAAA GGCAGTGAAA
    GAGAGCAAGA AGGACCAGAA AAAGAAAATG CGTACTGATC ATAAAGTCAA AGAACTCAAA
    ATACAGGGAA CTGCACAACA AGAGTTAAAT CAGATACTTT CTGAGAGAAC AAGCAGGGAT
    CCCCACAGTG TTGATTCAAA TGCAGCAGCA AGCACTGAGC ATAGTTTGGA GAATCAGCAG
    GTCACCGGAA ATATATCCTT CAGGGTCAGC CTAAAAGACA ATGACTTGAC AATGATTGAT
    GAGACGTCTT GGAATGTCAC AGTAGTTGAA ACTGATAAAG CAGATCAAAC TGCATCACAG
    GAGGATGAAG AATACTTGGA GGTGCCAGAA ACTGAGCAGA CCATCACCAG TTCCATTAAC
    TCATCAGAAC ATATGGTACA CAATAGAATC AGGAGGATGG CAAAAGATTT AGAAAGGATA
    TTCGCCCCAG AGAAACTAAG AAGACAAAGG AGAACAAAGG AAGGAAAGGG GCGTTCAGGA
    TCTCAAAGAG ACAAAGGACA AAAGGGTGAA CCGGGGGAGG CTGGTGAGGC TGGGATTCCT
    GGTCTGAAGG GTGAAAAGGG AGATATTGGA GAACAGGGAC AAAAGGGGGA ACCTGGTGTT
    GGACACCGTG GGCCAATTGG ACAGGCTGGA CCACCAGGTG TTAAGGGTGA GCCAGGAGAG
    ACGGGACCCC CAGGAGCTCA AGGAATTCAA GGTATCCAAG GCAACCCAGG CATCTCAGGA
    TTCCAAGGTG AAAGAGGAGA AACAGGATTT TCAGGGCCTC CTGGACCACC TGGAGAGAGA
    GGCAAGAAGG GAAAAAATGG AAATGCTGGT CAACCTGGGC CCCATGGATC AACAGGAAAA
    CAGGGGAAAC CAGGACTTCA AGGTAGTAAG GGAGAAAAGG GTGAAGCTGT GGTTGGTGAA
    CCTGGTCCTC GTGGGCCTCG CGGGCTTCCT GGCTTTAGGG GAGAAAATGG GATCCCAGGC
    TTACCTGGAC CTGCGGGAAT TGTAGGTTTA AAGGGATTCC CAGGAATAAA GGGTGAACGA
    GGTGATCGGG GGATTTTTGG GATTAAGGGA GAAAAGGGTG AATCCACAAC TCTCTTTGGC
    TCTCAAGGGT ATAAAGGAAA CAAAGGAGAT CCGGGTGACA GAGGCTACCC AGGCTTTGAT
    GGAGACAAAG GCGAGAAAGG AGAAGATGGA CCTGCTGGGG AAAAAGGTGT GAAAGGTGAA
    GCTGGTTCCA AAGGATCCAT GGGACTATTT GGAGCTCGGG GTCCTGTTGG ACAGAAGGGT
    GAGCCAGGGG AGCATGGACT TCCAGGAAGA GCAGGAACAG CAGGACTTGA TGGAAAAAAT
    GGACTCAAAG GAACAAAAGG TGACCGGGGT CTACAAGGAC AAAGGGGAAA CCCGGGAGAG
    AAAGGTGATT CTGGATTGTC AGGAGATCCT GGAAAGAAAG GAGATAAGGG TCTGCGGGGA
    ATTCCAGGAA AAATGGGAGA GCCTGGGACA GAAGGTGAAA AAGGTGACCC TGGGGCTCAA
    GGAAATCCCG GTACAGGATC AGAAGGTCCT TCTGGACCTA AGGGTGAGAA AGGTGATAAA
    GGTGAAGATG GACCCCCTGG AATTGCTGGA GATAAAGGAT TTAAGGGTAC TGATGGAGTG
    CGTGGACTGA ATGGCTTTAG GGGACCAATA GGCCATCCAG GAAAAGATGG CCCTCCAGGT
    CCTACTGGAC CTTCAGGACA GAAAGGAGAG CCAGGGCCAA AGGGTGAAAG AGGAAAAAGG
    GGAAAATCCT TGCAGTGTCC AAGAGGAACC CCAGGGGATC CAGGAAACAA AGGAGAAACG
    GGAGTGGAGG GGCCTGAAGG TCTCAGAGGG GACAAGGGAG ATCCAGGGCT CACAGAGGAA
    GAAGTGAAAG ATTTGGTCAG AAAAGAAATG AGCGACAAAT GTGCTTGTGC AGGAAAGGAT
    TTTCATCTAG TAATTAAATC ATCACTCCCA GATGAAGAGT ATGAAGATCC TGACCTAAAT
    GAAAGTCCAG TGACTGCTCC ACCACAGGAT ATTGTGACTG AAAATGATGT AGTAGTAGAA
    GCGTATGGAA ATGTAACACT TAATAGCACT TTAACTCCAG ATACGGAAGT GCAACAAAAC
    GACACAGACG GAAGTGATAA GGAGCTAATG GGACAATTGC AGAGAAGAAA ACGTGAAGCA
    GAAAATCTGT TTCGTAATCC CTGTTTGCTT CCAATGGATG AAGGATTCTG CTCCGATTAC
    ACCTTGGTTT GGTATTACCA CCGCAAAACA GACGTATGCC GGCCTTTTTT ATTTGGTGGT
    TGTGGGGGAA ATATCAATCG CTTTAAAACA AAAGACAGAT GTGAGCAGAG GTGTAAAACT
    CGATCAGTAT CATCCCGAGG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54929
    Clone ID Related Accession (Same CDS sequence) XM_031899081.1
    Accession Version XM_031899081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
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    1981
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    7741
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    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGTCTT GGAATGTCAC AGTAGTTGAA
    ACTGATAAAG CAGATCAAAC TGCATCACAG GAGGATGAAG AATACTTGGA GGTGCCAGAA
    ACTGAGCAGA CCATCACCAG TTCCATTAAC TCATCAGAAC ATATGGTACA CAATAGAATC
    AGGAGGATGG CAAAAGATTT AGAAAGGATA TTCGCCCCAG AGAAACTAAG AAGACAAAGG
    AGAACAAAGG AAGGAAAGGG GCGTTCAGGA TCTCAAAGAG ACAAAGGACA AAAGGGTGAA
    CCGGGGGAGG CTGGTGAGGC TGGGATTCCT GGTCTGAAGG GTGAAAAGGG AGATATTGGA
    GAACAGGGAC AAAAGGGGGA ACCTGGTGTT GGACACCGTG GGCCAATTGG ACAGGCTGGA
    CCACCAGGTG TTAAGGGTGA GCCAGGAGAG ACGGGACCCC CAGGAGCTCA AGGAATTCAA
    GGTATCCAAG GCAACCCAGG CATCTCAGGA TTCCAAGGTG AAAGAGGAGA AACAGGATTT
    TCAGGGCCTC CTGGACCACC TGGAGAGAGA GGCAAGAAGG GAAAAAATGG AAATGCTGGT
    CAACCTGGGC CCCATGGATC AACAGGAAAA CAGGGGAAAC CAGGACTTCA AGGTAGTAAG
    GGAGAAAAGG GTGAAGCTGT GGTTGGTGAA CCTGGTCCTC GTGGGCCTCG CGGGCTTCCT
    GGCTTTAGGG GAGAAAATGG GATCCCAGGC TTACCTGGAC CTGCGGGAAT TGTAGGTTTA
    AAGGGATTCC CAGGAATAAA GGGTGAACGA GGTGATCGGG GGATTTTTGG GATTAAGGGA
    GAAAAGGGTG AATCCACAAC TCTCTTTGGC TCTCAAGGGT ATAAAGGAAA CAAAGGAGAT
    CCGGGTGACA GAGGCTACCC AGGCTTTGAT GGAGACAAAG GCGAGAAAGG AGAAGATGGA
    CCTGCTGGGG AAAAAGGTGT GAAAGGTGAA GCTGGTTCCA AAGGATCCAT GGGACTATTT
    GGAGCTCGGG GTCCTGTTGG ACAGAAGGGT GAGCCAGGGG AGCATGGACT TCCAGGAAGA
    GCAGGAACAG CAGGACTTGA TGGAAAAAAT GGACTCAAAG GAACAAAAGG TGACCGGGGT
    CTACAAGGAC AAAGGGGAAA CCCGGGAGAG AAAGGTGATT CTGGATTGTC AGGAGATCCT
    GGAAAGAAAG GAGATAAGGG TCTGCGGGGA ATTCCAGGAA AAATGGGAGA GCCTGGGACA
    GAAGGTGAAA AAGGTGACCC TGGGGCTCAA GGAAATCCCG GTACAGGATC AGAAGGTCCT
    TCTGGACCTA AGGGTGAGAA AGGTGATAAA GGTGAAGATG GACCCCCTGG AATTGCTGGA
    GATAAAGGAT TTAAGGGTAC TGATGGAGTG CGTGGACTGA ATGGCTTTAG GGGACCAATA
    GGCCATCCAG GAAAAGATGG CCCTCCAGGT CCTACTGGAC CTTCAGGACA GAAAGGAGAG
    CCAGGGCCAA AGGGTGAAAG AGGAAAAAGG GGAAAATCCT TGCAGTGTCC AAGAGGAACC
    CCAGGGGATC CAGGAAACAA AGGAGAAACG GGAGTGGAGG GGCCTGAAGG TCTCAGAGGG
    GACAAGGGAG ATCCAGGGCT CACAGAGGAA GAAGTGAAAG ATTTGGTCAG AAAAGAAATG
    AGCGACAAAT GTGCTTGTGC AGGAAAGGAT TTTCATCTAG TAATTAAATC ATCACTCCCA
    GATGAAGAGT ATGAAGATCC TGACCTAAAT GAAAGTCCAG TGACTGCTCC ACCACAGGAT
    ATTGTGACTG AAAATGATGT AGTAGTAGAA GCGTATGGAA ATGTAACACT TAATAGCACT
    TTAACTCCAG ATACGGAAGT GCAACAAAAC GACACAGACG GAAGTGATAA GGAGCTAATG
    GGACAATTGC AGAGAAGAAA ACGTGAAGCA GAAAATCTGT TTCGTAATCC CTGTTTGCTT
    CCAATGGATG AAGGATTCTG CTCCGATTAC ACCTTGGTTT GGTATTACCA CCGCAAAACA
    GACGTATGCC GGCCTTTTTT ATTTGGTGGT TGTGGGGGAA ATATCAATCG CTTTAAAACA
    AAAGACAGAT GTGAGCAGAG GTGTAAAACT CGATCAGTAT CATCCCGAGG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754941.1
    CDS1389..9785
    Translation

    Target ORF information:

    RefSeq Version XM_031899081.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGTCTT GGAATGTCAC AGTAGTTGAA
    ACTGATAAAG CAGATCAAAC TGCATCACAG GAGGATGAAG AATACTTGGA GGTGCCAGAA
    ACTGAGCAGA CCATCACCAG TTCCATTAAC TCATCAGAAC ATATGGTACA CAATAGAATC
    AGGAGGATGG CAAAAGATTT AGAAAGGATA TTCGCCCCAG AGAAACTAAG AAGACAAAGG
    AGAACAAAGG AAGGAAAGGG GCGTTCAGGA TCTCAAAGAG ACAAAGGACA AAAGGGTGAA
    CCGGGGGAGG CTGGTGAGGC TGGGATTCCT GGTCTGAAGG GTGAAAAGGG AGATATTGGA
    GAACAGGGAC AAAAGGGGGA ACCTGGTGTT GGACACCGTG GGCCAATTGG ACAGGCTGGA
    CCACCAGGTG TTAAGGGTGA GCCAGGAGAG ACGGGACCCC CAGGAGCTCA AGGAATTCAA
    GGTATCCAAG GCAACCCAGG CATCTCAGGA TTCCAAGGTG AAAGAGGAGA AACAGGATTT
    TCAGGGCCTC CTGGACCACC TGGAGAGAGA GGCAAGAAGG GAAAAAATGG AAATGCTGGT
    CAACCTGGGC CCCATGGATC AACAGGAAAA CAGGGGAAAC CAGGACTTCA AGGTAGTAAG
    GGAGAAAAGG GTGAAGCTGT GGTTGGTGAA CCTGGTCCTC GTGGGCCTCG CGGGCTTCCT
    GGCTTTAGGG GAGAAAATGG GATCCCAGGC TTACCTGGAC CTGCGGGAAT TGTAGGTTTA
    AAGGGATTCC CAGGAATAAA GGGTGAACGA GGTGATCGGG GGATTTTTGG GATTAAGGGA
    GAAAAGGGTG AATCCACAAC TCTCTTTGGC TCTCAAGGGT ATAAAGGAAA CAAAGGAGAT
    CCGGGTGACA GAGGCTACCC AGGCTTTGAT GGAGACAAAG GCGAGAAAGG AGAAGATGGA
    CCTGCTGGGG AAAAAGGTGT GAAAGGTGAA GCTGGTTCCA AAGGATCCAT GGGACTATTT
    GGAGCTCGGG GTCCTGTTGG ACAGAAGGGT GAGCCAGGGG AGCATGGACT TCCAGGAAGA
    GCAGGAACAG CAGGACTTGA TGGAAAAAAT GGACTCAAAG GAACAAAAGG TGACCGGGGT
    CTACAAGGAC AAAGGGGAAA CCCGGGAGAG AAAGGTGATT CTGGATTGTC AGGAGATCCT
    GGAAAGAAAG GAGATAAGGG TCTGCGGGGA ATTCCAGGAA AAATGGGAGA GCCTGGGACA
    GAAGGTGAAA AAGGTGACCC TGGGGCTCAA GGAAATCCCG GTACAGGATC AGAAGGTCCT
    TCTGGACCTA AGGGTGAGAA AGGTGATAAA GGTGAAGATG GACCCCCTGG AATTGCTGGA
    GATAAAGGAT TTAAGGGTAC TGATGGAGTG CGTGGACTGA ATGGCTTTAG GGGACCAATA
    GGCCATCCAG GAAAAGATGG CCCTCCAGGT CCTACTGGAC CTTCAGGACA GAAAGGAGAG
    CCAGGGCCAA AGGGTGAAAG AGGAAAAAGG GGAAAATCCT TGCAGTGTCC AAGAGGAACC
    CCAGGGGATC CAGGAAACAA AGGAGAAACG GGAGTGGAGG GGCCTGAAGG TCTCAGAGGG
    GACAAGGGAG ATCCAGGGCT CACAGAGGAA GAAGTGAAAG ATTTGGTCAG AAAAGAAATG
    AGCGACAAAT GTGCTTGTGC AGGAAAGGAT TTTCATCTAG TAATTAAATC ATCACTCCCA
    GATGAAGAGT ATGAAGATCC TGACCTAAAT GAAAGTCCAG TGACTGCTCC ACCACAGGAT
    ATTGTGACTG AAAATGATGT AGTAGTAGAA GCGTATGGAA ATGTAACACT TAATAGCACT
    TTAACTCCAG ATACGGAAGT GCAACAAAAC GACACAGACG GAAGTGATAA GGAGCTAATG
    GGACAATTGC AGAGAAGAAA ACGTGAAGCA GAAAATCTGT TTCGTAATCC CTGTTTGCTT
    CCAATGGATG AAGGATTCTG CTCCGATTAC ACCTTGGTTT GGTATTACCA CCGCAAAACA
    GACGTATGCC GGCCTTTTTT ATTTGGTGGT TGTGGGGGAA ATATCAATCG CTTTAAAACA
    AAAGACAGAT GTGAGCAGAG GTGTAAAACT CGATCAGTAT CATCCCGAGG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54930
    Clone ID Related Accession (Same CDS sequence) XM_031899082.1
    Accession Version XM_031899082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(VII) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGGTGACA GAGGCTACCC AGGCTTTGAT
    GGAGACAAAG GCGAGAAAGG AGAAGATGGA CCTGCTGGGG AAAAAGGTGT GAAAGGTGAA
    GCTGGTTCCA AAGGATCCAT GGGACTATTT GGAGCTCGGG GTCCTGTTGG ACAGAAGGGT
    GAGCCAGGGG AGCATGGACT TCCAGGAAGA GCAGGAACAG CAGGACTTGA TGGAAAAAAT
    GGACTCAAAG GAACAAAAGG TGACCGGGGT CTACAAGGAC AAAGGGGAAA CCCGGGAGAG
    AAAGGTGATT CTGGATTGTC AGGAGATCCT GGAAAGAAAG GAGATAAGGG TCTGCGGGGA
    ATTCCAGGAA AAATGGGAGA GCCTGGGACA GAAGGTGAAA AAGGTGACCC TGGGGCTCAA
    GGAAATCCCG GTACAGGATC AGAAGGTCCT TCTGGACCTA AGGGTGAGAA AGGTGATAAA
    GGTGAAGATG GACCCCCTGG AATTGCTGGA GATAAAGGAT TTAAGGGTAC TGATGGAGTG
    CGTGGACTGA ATGGCTTTAG GGGACCAATA GGCCATCCAG GAAAAGATGG CCCTCCAGGT
    CCTACTGGAC CTTCAGGACA GAAAGGAGAG CCAGGGCCAA AGGGTGAAAG AGGAAAAAGG
    GGAAAATCCT TGCAGTGTCC AAGAGGAACC CCAGGGGATC CAGGAAACAA AGGAGAAACG
    GGAGTGGAGG GGCCTGAAGG TCTCAGAGGG GACAAGGGAG ATCCAGGGCT CACAGAGGAA
    GAAGTGAAAG ATTTGGTCAG AAAAGAAATG AGCGACAAAT GTGCTTGTGC AGGAAAGGAT
    TTTCATCTAG TAATTAAATC ATCACTCCCA GATGAAGAGT ATGAAGATCC TGACCTAAAT
    GAAAGTCCAG TGACTGCTCC ACCACAGGAT ATTGTGACTG AAAATGATGT AGTAGTAGAA
    GCGTATGGAA ATGTAACACT TAATAGCACT TTAACTCCAG ATACGGAAGT GCAACAAAAC
    GACACAGACG GAAGTGATAA GGAGCTAATG GGACAATTGC AGAGAAGAAA ACGTGAAGCA
    GAAAATCTGT TTCGTAATCC CTGTTTGCTT CCAATGGATG AAGGATTCTG CTCCGATTAC
    ACCTTGGTTT GGTATTACCA CCGCAAAACA GACGTATGCC GGCCTTTTTT ATTTGGTGGT
    TGTGGGGGAA ATATCAATCG CTTTAAAACA AAAGACAGAT GTGAGCAGAG GTGTAAAACT
    CGATCAGTAT CATCCCGAGG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754942.1
    CDS1389..9695
    Translation

    Target ORF information:

    RefSeq Version XM_031899082.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen alpha-1(VII) chain (LOC101732997), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGAATTTAT TGTCATGCTT GTGGAGTTTC ACTGTAACTG CACTTTTAAC AGAATGGACA 
    GTGTGTGCTC AACCAGAAGA GTGCAGATCA GCAGAAAAGG CAGACATCTT ATTTTTGGTG
    GATGAATCTT GGAGTGTTGG AGAAGGAAAT TTTCAACTGA TTAAAGACTT TATCAGAAGT
    ATAATGAGAT CATTTCAGAA CACAGAGATA CTTGGAAAAG AAGGATTGCG TTTTGGAGTA
    GCTGTTTATG GAGACATTGT TAGGATGAGC ATTGAGCTAT CAGACTATGA AAACACAAAA
    GAAATATTAC TGGCTCTCCA AAATATTGTA TATAAAGGAG GTAACACAAA GACTGGAGAG
    GCTCTTGCTT TCCTTGCAGA AAGAGTATTT AATCCCTCAA TTTCCAGAGA AGATGCAGCT
    AAGATAGTCT TGCTTCTAAC AGATGGAAAA TCTGGTGATT TAGTGGAAGA GAAAGCAAAA
    CTATTGCAGG ACAGAGGTGT TACAATCTTT ACAATTGGTA TTAAAAATGC AGACCGGAGT
    GAGCTGATGA AAATTGCATC TGAACCAACT GATGAACATG TTATACTAGT GAATGATTTC
    TGGTTTCTAA ACAATTTTCT GCCCAAAATT TCCCGGAGAA TATGTTTTGC TGCTTCTGAG
    CCACCAAGGC CACTCAGACA AATTGTGGAA GCAGAAAAAG TAATTGGCCC TAAAGATCTG
    ATTATAAGTG AGGAAAGTTA CAACTCTTTG CGTCTCACTT GGACTGCAGC CACAGGAGAT
    GCTACTGGAT ATCAAGTTGT CTTGAATTCC CTTACAGATT CAGGGCATCT TAATTCCCAT
    GACCAAAAGC AGATTGTTGT GGACAAGGAC AAGCATACTA TTATTGTGTC GGATCTAAGG
    CCAAACACAG AATATTTATT TACAGTACTT GCTCTCTACT CTAATTTCAT AGGGGATTCA
    GCCACAATTC GTGGAAGGAC AACTCCTATT CCACCTGTGA CAAATTTTCG AGTCATTGAA
    GAGGGACTTT TTAGCTTAAA GCTGGCCTGG ACTCCTTCTC TGGGGAAACT GGATGGTTAT
    AAGATCTTTA TTCCAAGATC CAACAGACCT GGATTAACAT ATGAACAGAT TGTGAGTGGA
    GATGTCTTCT CACACACTAT TGACAATCTA GAGGAAGATA AAGAATACAC TGTCAGTATA
    TATGCTGTAT ATCCAGAAGG ACCCAGTAAC CCTGTCTCAG CAGTTGGACA CACATTAAAG
    CTGCTTGCTG TGAAAAGCCT CTCCCTGCAG AACATCACAA CAGGCACTAT ACGAGCACAA
    TGGACTACAG TACATGGAGC AACTGGGTAT CGACTGACTT GGTCCTCTGC AGAGGGCAAT
    GACCAAAATG TTAACCTAGC TGATAGCTAT TCCCAGTATA TGATACAAGG GCTTCAGGCG
    GACACCGAGT ACACAGTGAC GATTAATCCT ATCTTTGTGG ACATAGAGGG TCCTGTTGTA
    ACAGCAAAAG CTACAACCTT GGCATCTAGT GCAGTACAAA GTCTTAAAAT AAGTGCAGTA
    ACTATAAGCA CTGCAGTGGT GTCATGGAAT GCTGTGCCAG GAGCTACAGG ATACAGACTT
    GCATGGGGTC CCACACCAGA ATTTATTGGT AAGGACCGTC CTCGCCAGCT AGCCCTCAAT
    AGTTCAATCA CAACCTACCA ACTGAAACAC CTTGTTCACA ATACGGAATA TGTCCTTTCA
    TTATATGTAC TCTTTGGCTC TGTCGTAGGG CCTGGAATTT CAACCACACT CAGGACATCT
    CCACTTGGAT ATGTTTCTAA CTTTAAGGTG ACAGGATATA CGAGTACCTC TATCTCGCTT
    GCTTGGAGTT ACACTATTGG AGCTACTGAA TATAAAATTT CCTGGAGCAC CACAACATCA
    GGTTTGCCAC TGAACACTCC TAAAAGTCAT TATCTGGCGT CCGGTGTCAG GTCCTATCAC
    ATCACAAATT TGTACCCAAA CACAAAGTAC ACTGTGAATG TTCAGGCAGT ATATAATAAC
    TTTGAAGGGC CGCCAAGCAC TCTAATTCAA ACCACAGACT CAAATCCTGT GCAGCTACTG
    CCTGTGAAAG ATCTGCAGAT CATTGATACT GGAGTCAATA TGCTGAAACT GTCTTGGAAG
    AAAACACCTG GAATAACACA TTATAAGATC TCATGGACAC CATTTGATGG TGGTGCAAGA
    AACTCTAAGA TTGTCTCTGC TGGTACGTCA TTTTTCACAA TCCCTGACCT TAAGGAGAGT
    TCCACTTACA GCATCTATGT ATCCTCGATA ATCGGGAATC GGGAAGGAAG TTCAGTGGTT
    CTTACTGCAA AAACATTGGA TTTGCCAAAA GTAGAATCTT TTGAAGTCAA AGAGACCACA
    GAGGACAGTG TTTTATTAAG CTGGACTGGA GTCACTGGAG CATCTTCATA TATTTTAACA
    TGGAGGATGG CTTCAGACCC AGAATCCTCC GTGGAGCAGT TAGCTCCAAC ATATAGATCT
    TTCCGGCTCA TGAATTTGGA GAATGGAAAG TCATATATTT TTGCAATAAA GCCTCTTTTT
    GGAGAGATGG AAGGGCCTGA GAGAACAATC ACACACACAA TCGTTGGACC CCCCAAAAAA
    CAGATCTACC CTTCCCCTAC CCTTCAAATA CCTGTTCCAA CACCTCAAAG GACCCTAACA
    ACACAAGTTA TCAACCACCC TCCCATAGTC ACCACAACTG AAGCTGCAAA ATCCACAAGC
    ACTGTCTCAC ATGGTTCTTC CACAACTACT TCTGAACCCA TTTGTGCAAA ACTGAAAGCT
    GACATAGTAT TTCTGGTTGA TGAGTCCTCC AGCATTGGCC CATCCAATTT TATTAAGGTT
    AAGGATTTCC TCTACAGAAT TGTCTCTTAC TTTCCCAAAA TTGGACCTCA AGGAACACAG
    ATTGCTATTG TTCAGTACAG CGAAGAACCC AGAACAGAAT TCCACTTGAA TGAGTATAAG
    GAGAGAAATT CTGTTTTGAA AGCAATCAGA GCTCTACAGT ATTATGGAGG CAACACCAAG
    ACTGGTCGTG GTATAGGACA TGCACTGAAG GAAATGTTTC AGGTTTCCAA AGGGATGAGA
    CCATCTGCTC CACATATACT TGTGCTAATA ACTGACGGAA GGTCTCAGGA TAATGTAATA
    CAGCCAGCCA AAGTAGCTCA TGTATTAGGC ATTCGTATGA TTGCAGTCGG TGTGGCTGGG
    GCAGATATGG ATGAACTGAA AGGAATGATG ATGAACCGTA ATTTAGAAAA CATCTTCTTT
    GCCAGTACAT TTGATGATTT TCCTCTAATT GTACAAGATC TGATAGATGC TATTTGCACT
    GAAACCGAAC ATGCAGTTAA AGAAGAACCT ACTAAGTTCA GAGCACCAGA TAAAGAAGAG
    GAGCCAGAAA GACCAAAACC TCCTCCTGGG AATCGAGAGG GACCCTGTGG AAATACCATG
    TGCCCAACGG GGCAAAAGGG ACAAAAGGGA GAAAAGGGTC CACCGGGAGA TTCAGTTTTC
    ACAGGTTTAC AGACTGGTGG CTTTGACCCT TTTGATTTGA ATTCAAAAGG AGAAAAAGGA
    GAGAGAGGTC TTCCTGGACA AGATGGTGTT CCTGGCCTTC CAGGACGTCC TGGTCGAACT
    GGGCCCCCAG GGCCACCTGG CCTTATGGGA CAAAAAGGAT TTCAAGGCGA TATTGGCCCC
    CAAGGATATC CAGGTCAGCC AGGTCCCAAA GGAGACAGGG GAGAACCAGG CTATATACTA
    GGAGGAGTTG ATGGAATGGC TGGTCTAAGT GCAATACCTG GGTCTCCTGG ACCCAAAGGA
    CAGCCTGGGG TTCCAGGGCT ACCAGGACCA CCTGGACTTC CAGGATTTCC TGGACCTCAG
    GGACCTCCAG GAACTTCTAT AAAGGGGGAA CCTGGTGAAA CAGGGAGCCA GGGTCCTCGT
    GGAAAAAGTG GACCAAAAGG AGATAAAGGA GACCAGGGGG ATGCAGGAAG AGCTGGCTTA
    CCTGGCCCAA TTGGGCTTGA TGGCTCTCCA GGAATTCATG GAATTAAAGG AGACAAGGGG
    GAGGCTGGTA TTGGAATCCC TGGATTACCT GGTTTAAAGG GTATAACAGG TGAAAAGGGT
    GTCATTGGAC TTCCTGGCCC TCAAGGACAG AAGGGTACTC AAGGAATACA AGGTGTAGAA
    GGTGTCGCAG GTTTAAGGGG TAAAAAAGGT CAAGATGGTG AAAAGGGTGA AAAGGGAGAA
    CATGGTGCTA TGGGAATGAC TGGTCCTCCT GGAATAGCTG GTCTCTCTGG ACCAATGGGG
    CAAAAGGGGG ATCAGGGGTT GCAAGGTTTT CCTGGCGCTC CTGCTATGGG GGTAATTGGG
    CCTTCTGGTA AAAAAGGTGC CAGAGGTGAT ATTGGACCAG TTGGGCCAAT TGGACCTAAA
    GGCATTATGG GAAGTCAAGG AGATAAAGGA ATTAAGGGAG AACCAGGATA TGGTATACCT
    GGGCAGCCTG GATTAAAGGG AGATCCAGGT GACAGAGGAA ACATAGGATT ATCTGGAAAA
    CCAGGTCCCA AAGGAGATTT TGGAGACAAA GGACAGAAGG GTGAGCCTGG AAACTTTGGT
    GCCCTAGGAC AGTCTGGACT AAGAGGTAAA GATGGTGAAC CAGGACTAAA TGGGGACAAA
    GGCGAGAAGG GAGACGCAGG TGTCCCCGGA GAACCAGGGG AAAAGGGACA AAGGGGCTCT
    GTTGGACTGC CTGGCCGGAT TGGTGATACT GGAATAAAAG GAGATATGGG GCCACCTGGA
    AGAGATGGCA TAGATAGTAA AAAAGGTGAT AAAGGAGAAG CCGGGCAGCC AGGAACACCT
    GGGGCAATGG TGACTCAAAT AGACAAAAGT GTGCTTGTCA AAGGAGAAAA GGGAGAACCC
    GGAGAGGTGG GAGATCCAGG AGAATCGGGT TTTAAAGGAG AAAAAGGAGA CACGGGGCTA
    CCAGGGCCCA GAGGACCTGA AGGCCGTCCA GGACCACCTG GTGCTTCACC GTTGGAGTCC
    GCTTCTGAGA AAGGAATGAA AGGGGAAAAG GGAAGCCCAG GTTCTTCTGG AAAAAATGGC
    CTTGATGGAA AGCCTGGGAA TCCTGGGTTA CTTGGACAAA AGGGAGAAGC AGGCCAAAAA
    GGAGAACCAG GAAATGTAGG CCTAACTGTA AGTGAAAAGG GAAATAAAGG AGAAATAGGA
    AACCAAGGCC CACCAGGACC TCAGGGACCT CCTTGCACAG ATTTAGAAAT GAAGGATTTC
    GAAAGGCTAT TTGAATCTTA TGGCATTAAG CTGTCTCTAC TGAAGGAACT GACTGATCAT
    ATTATACAAA ATGGATTAGC TGATGTAATT CAGCAACTAA CAAATTCCAA GAAAGACCGA
    ACTTCTAAGA AGAAGCAAGT TTATAAACAG TCACCAGAAT TCACTGATGC CTTAAAACAA
    GATCATATTA ATGAGGCAGA AAAAAACCAT GAGGAAGAAG AAGATCCAAG CTTGCAGGCT
    GAGATAATAC CTACAGTGGG GGTGACTGAG TCCCATACTC TAACTCAGGA CTTAAAAGAA
    GGCAATAAAG AACAAAAGAA AAAGAAGAAA ACACATGTAG AGGCTGTTCC TATTAACATT
    TACACAAATG TGATCACTGA GGAACAGGAG GAAATCAGCC AAGACCAGAG CCACCATTTA
    ACAACCAATA CTACTTTTTT GCCAACTCAG CAATCAATAG ATTTGGAAGG ATCTGATGCA
    AATGCTGATA GAGTTGTCCA AGCACAGATT GACAAACATC AGAGGAAGCA AGAGAAAGAG
    GTTAGAAAGG AAAGGAAAAA AAATCGCCTT GAAACTACAG CTACTGAAAC AGAAATGAAG
    AACCTTGTTT TGGAGGAAAT TACAGCAGTG ACTGCTTATA AAGAAGAAAA TTCACAATCT
    GCCACCACCC TGCCTGCAAT AATTAATGCA AAGAGGTTAA CATCTGACTT GATAGAGGAG
    ACAGACACAA TCGTTCAGCC CACATCTGAT ACTACTATAC TAGTCGAAAA GCATCAGGAA
    AAGGCAGTGA AAGAGAGCAA GAAGGACCAG AAAAAGAAAA TGCGTACTGA TCATAAAGTC
    AAAGAACTCA AAATACAGGG AACTGCACAA CAAGAGTTAA ATCAGATACT TTCTGAGAGA
    ACAAGCAGGG ATCCCCACAG TGTTGATTCA AATGCAGCAG CAAGCACTGA GCATAGTTTG
    GAGAATCAGC AGGTCACCGG AAATATATCC TTCAGGGTCA GCCTAAAAGA CAATGACTTG
    ACAATGATTG ATGAGACGTC TTGGAATGTC ACAGTAGTTG AAACTGATAA AGCAGATCAA
    ACTGCATCAC AGGAGGATGA AGAATACTTG GAGGTGCCAG AAACTGAGCA GACCATCACC
    AGTTCCATTA ACTCATCAGA ACATATGGTA CACAATAGAA TCAGGAGGAT GGCAAAAGAT
    TTAGAAAGGA TATTCGCCCC AGAGAAACTA AGAAGACAAA GGAGAACAAA GGAAGGAAAG
    GGGCGTTCAG GATCTCAAAG AGACAAAGGA CAAAAGGGTG AACCGGGGGA GGCTGGTGAG
    GCTGGGATTC CTGGTCTGAA GGGTGAAAAG GGAGATATTG GAGAACAGGG ACAAAAGGGG
    GAACCTGGTG TTGGACACCG TGGGCCAATT GGACAGGCTG GACCACCAGG TGTTAAGGGT
    GAGCCAGGAG AGACGGGACC CCCAGGAGCT CAAGGAATTC AAGGTATCCA AGGCAACCCA
    GGCATCTCAG GATTCCAAGG TGAAAGAGGA GAAACAGGAT TTTCAGGGCC TCCTGGACCA
    CCTGGAGAGA GAGGCAAGAA GGGAAAAAAT GGAAATGCTG GTCAACCTGG GCCCCATGGA
    TCAACAGGAA AACAGGGGAA ACCAGGACTT CAAGGTAGTA AGGGAGAAAA GGGTGAAGCT
    GTGGTTGGTG AACCTGGTCC TCGTGGGCCT CGCGGGCTTC CTGGCTTTAG GGGAGAAAAT
    GGGATCCCAG GCTTACCTGG ACCTGCGGGA ATTGGTGACA GAGGCTACCC AGGCTTTGAT
    GGAGACAAAG GCGAGAAAGG AGAAGATGGA CCTGCTGGGG AAAAAGGTGT GAAAGGTGAA
    GCTGGTTCCA AAGGATCCAT GGGACTATTT GGAGCTCGGG GTCCTGTTGG ACAGAAGGGT
    GAGCCAGGGG AGCATGGACT TCCAGGAAGA GCAGGAACAG CAGGACTTGA TGGAAAAAAT
    GGACTCAAAG GAACAAAAGG TGACCGGGGT CTACAAGGAC AAAGGGGAAA CCCGGGAGAG
    AAAGGTGATT CTGGATTGTC AGGAGATCCT GGAAAGAAAG GAGATAAGGG TCTGCGGGGA
    ATTCCAGGAA AAATGGGAGA GCCTGGGACA GAAGGTGAAA AAGGTGACCC TGGGGCTCAA
    GGAAATCCCG GTACAGGATC AGAAGGTCCT TCTGGACCTA AGGGTGAGAA AGGTGATAAA
    GGTGAAGATG GACCCCCTGG AATTGCTGGA GATAAAGGAT TTAAGGGTAC TGATGGAGTG
    CGTGGACTGA ATGGCTTTAG GGGACCAATA GGCCATCCAG GAAAAGATGG CCCTCCAGGT
    CCTACTGGAC CTTCAGGACA GAAAGGAGAG CCAGGGCCAA AGGGTGAAAG AGGAAAAAGG
    GGAAAATCCT TGCAGTGTCC AAGAGGAACC CCAGGGGATC CAGGAAACAA AGGAGAAACG
    GGAGTGGAGG GGCCTGAAGG TCTCAGAGGG GACAAGGGAG ATCCAGGGCT CACAGAGGAA
    GAAGTGAAAG ATTTGGTCAG AAAAGAAATG AGCGACAAAT GTGCTTGTGC AGGAAAGGAT
    TTTCATCTAG TAATTAAATC ATCACTCCCA GATGAAGAGT ATGAAGATCC TGACCTAAAT
    GAAAGTCCAG TGACTGCTCC ACCACAGGAT ATTGTGACTG AAAATGATGT AGTAGTAGAA
    GCGTATGGAA ATGTAACACT TAATAGCACT TTAACTCCAG ATACGGAAGT GCAACAAAAC
    GACACAGACG GAAGTGATAA GGAGCTAATG GGACAATTGC AGAGAAGAAA ACGTGAAGCA
    GAAAATCTGT TTCGTAATCC CTGTTTGCTT CCAATGGATG AAGGATTCTG CTCCGATTAC
    ACCTTGGTTT GGTATTACCA CCGCAAAACA GACGTATGCC GGCCTTTTTT ATTTGGTGGT
    TGTGGGGGAA ATATCAATCG CTTTAAAACA AAAGACAGAT GTGAGCAGAG GTGTAAAACT
    CGATCAGTAT CATCCCGAGG GAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.