astl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following astl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the astl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43559 XM_018092670.1
Latest version!
Xenopus tropicalis astacin like metalloendopeptidase (astl), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OXa37305 XM_031899276.1
Latest version!
Xenopus tropicalis astacin-like metallo-endopeptidase (M12 family) (astl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa55292 XM_031899277.1
Latest version!
Xenopus tropicalis astacin-like metallo-endopeptidase (M12 family) (astl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43559
    Clone ID Related Accession (Same CDS sequence) XM_018092670.1
    Accession Version XM_018092670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product astacin-like metalloendopeptidase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGGGCTG TTTTGGCAAT ATGGCTGCTC GGAGCTGCAG GTGCGCTTGG GTTTCCAGTG 
    CCCAACCCGG CTCCGTCGGC AAGCTCTGAA GGAACAAACG CGAGCAATGC TGGCATCCTG
    GACATCAATG GGCCTCTCAT TCAAGGCGGT GCTTCAGGCC TGATGGAAGG GGACATTGTG
    AAAGAAAAGA GATCTATCCG CACATTCTCT GCTCGATTTG CAAAGTGGCC TAAGATAAAT
    GGCTCTGTGA TTATCCCCTA CACTCTGTCT TCTTCCTATG AAAGTTTTGA CCGGAATATA
    ATTCTAAAGG CCTTTCGGGA CTTGCAAGCA TCTACTTGCC TCCGCTTTGT GGAGCGCACT
    ACTGAACGGG ATTATATCGC CATAGAGCCA GCTATTGGGT GTTTTTCGAG TGTAGGGCGA
    GTTGGGGGGA TGCAGCTCGT CTCCCTGGCC TTTGAGTGCC TGAGGACAGA CAAGGGAAAG
    GGAATTGCTC TGCATGAATT GATGCACGTC GCTGGGTTTT GGCATGAACA TTCCAGAGCT
    GATCGAGATG ATTACATCTG GATAATTTGG GATGAGATTC TAATCGGCTA TGAGAAAAAC
    TTCTGTAAAT ATGCGACTAC CAACATGTTA GTCAAATATG AGCTGCAGTC CATCCTTCAT
    TACCCCAGAA GTGCATTCTC CAAGTCTGGG CAGGCCACAA TCAATCCAAA GTATCCATAT
    TCTAAGATTG AAATAGGTCA GAGAGAAAAA CTCAGTGCTT CTGACATCCT AAGGGTTAAC
    AAACTGTACT CCTGCTCTCA GTACACATCA TCAGATGGCA AGTCATTTTT TTTAAATTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948159.1
    CDS973..1815
    Translation

    Target ORF information:

    RefSeq Version XM_018092670.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis astacin like metalloendopeptidase (astl), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGGGCTG TTTTGGCAAT ATGGCTGCTC GGAGCTGCAG GTGCGCTTGG GTTTCCAGTG 
    CCCAACCCGG CTCCGTCGGC AAGCTCTGAA GGAACAAACG CGAGCAATGC TGGCATCCTG
    GACATCAATG GGCCTCTCAT TCAAGGCGGT GCTTCAGGCC TGATGGAAGG GGACATTGTG
    AAAGAAAAGA GATCTATCCG CACATTCTCT GCTCGATTTG CAAAGTGGCC TAAGATAAAT
    GGCTCTGTGA TTATCCCCTA CACTCTGTCT TCTTCCTATG AAAGTTTTGA CCGGAATATA
    ATTCTAAAGG CCTTTCGGGA CTTGCAAGCA TCTACTTGCC TCCGCTTTGT GGAGCGCACT
    ACTGAACGGG ATTATATCGC CATAGAGCCA GCTATTGGGT GTTTTTCGAG TGTAGGGCGA
    GTTGGGGGGA TGCAGCTCGT CTCCCTGGCC TTTGAGTGCC TGAGGACAGA CAAGGGAAAG
    GGAATTGCTC TGCATGAATT GATGCACGTC GCTGGGTTTT GGCATGAACA TTCCAGAGCT
    GATCGAGATG ATTACATCTG GATAATTTGG GATGAGATTC TAATCGGCTA TGAGAAAAAC
    TTCTGTAAAT ATGCGACTAC CAACATGTTA GTCAAATATG AGCTGCAGTC CATCCTTCAT
    TACCCCAGAA GTGCATTCTC CAAGTCTGGG CAGGCCACAA TCAATCCAAA GTATCCATAT
    TCTAAGATTG AAATAGGTCA GAGAGAAAAA CTCAGTGCTT CTGACATCCT AAGGGTTAAC
    AAACTGTACT CCTGCTCTCA GTACACATCA TCAGATGGCA AGTCATTTTT TTTAAATTTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37305
    Clone ID Related Accession (Same CDS sequence) XM_031899276.1
    Accession Version XM_031899276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product astacin-like metalloendopeptidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGCTG TTTTGGCAAT ATGGCTGCTC GGAGCTGCAG GTGCGCTTGG GTTTCCAGTG 
    CCCAACCCGG CTCCGTCGGC AAGCTCTGAA GGAACAAACG CGAGCAATGC TGGCATCCTG
    GACATCAATG GGCCTCTCAT TCAAGGCGGT GCTTCAGGCC TGATGGAAGG GGACATTGTG
    AAAGAAAAGA GATCTATCCG CACATTCTCT GCTCGATTTG CAAAGTGGCC TAAGATAAAT
    GGCTCTGTGA TTATCCCCTA CACTCTGTCT TCTTCCTATG AAAGTTTTGA CCGGAATATA
    ATTCTAAAGG CCTTTCGGGA CTTGCAAGCA TCTACTTGCC TCCGCTTTGT GGAGCGCACT
    ACTGAACGGG ATTATATCGC CATAGAGCCA GCTATTGGGT GTTTTTCGAG TGTAGGGCGA
    GTTGGGGGGA TGCAGCTCGT CTCCCTGGCC TTTGAGTGCC TGAGGACAGA CAAGGGAAAG
    GGAATTGCTC TGCATGAATT GATGCACGTC GCTGGGTTTT GGCATGAACA TTCCAGAGCT
    GATCGAGATG ATTACATCTG GATAATTTGG GATGAGATTC TAATCGGCTA TGAGAAAAAC
    TTCTGTAAAT ATGCGACTAC CAACATGTTA GTCAAATATG AGCTGCAGTC CATCCTTCAT
    TACCCCAGAA GTGCATTCTC CAAGTCTGGG CAGGCCACAA TCAATCCAAA GTATCCATAT
    TCTAAGATTG AAATAGGTCA GAGAGAAAAA CTCAGTGCTT CTGACATCCT AAGGGTTAAC
    AAACTGTACT CCTGCTCTCA GTACACATCA TCAGATGATG ATGATGATGA ATTAAATTAT
    TTTCCACCAA TTGTTTATTC TTGCAACAAA GAACTCAAGC CTACATCCAC TAAATCATAT
    ATTGAACATA AGAATGGAGC TTTGCTAATG GAGACCCCAG CACAGATGGC CAATACCAAA
    CAAACATCCG TTTTAAGTCA AACAGCAGCT CCTACTGTGA GTTTTCAGAG AAGTTCAGTG
    AATGAAGGAA CTGCAATAGC CGGGCATAAA CATACAACTC TTTTAAGTAG TGTAAATATG
    ACTGGGGATT TTCAAGATAG TGTTTCTACA GGCTTTTACC AAACATCTTC TGAAACCAAT
    TCTTCTCAGT CATTAAATGA GCAATTGCAG GAAACACTAC AAAAGTCAAC AATGAGGTCT
    AATGAGGGTT TATTTACATC AGCCCCTACA ACACTGCCAT CTCATGTGAT TAATTTGAGC
    TCCCTCAATG CTTTTTCCAC CCTGGTAAAC CTGTCTGTGC TACCCACCAC CACCGTCACA
    CCTGAGCCTA TTAAAATACC TTCTAACAGT AATTTTTCTC AAATTGTAAC CAACATCATG
    CAAAAAACTC AACCTTCAGT TTCTACTTTG GCATCAAACT CTATATCACC TAACACTGTG
    GAACTCACTC ACAATGCTTT GATACCAACC AAAATCAGTC GCCACCAACA CAGAACAGAT
    GAAACCAGTA CCGTACCTGA GCAAGTGACT CTTCTCCATG TCAATGAAAG TACTGAACAT
    GGCACACCTA CAGAGTCCAA CCAAATTCTT ACTGAAAATG TTTCTTCCAA GCCAACTCCC
    TATCCCCCTG AGCACTTAGA CTACTCAAAC CTGACTAAAT CAGAAGGGGA AAATCTCAAT
    TATGTCATAA ACAGTTCGGA CACCTCTACA ACATCTATAA ATCTCGTTTC TGAAAGCATA
    TCCTCTCGGG CACCTCTGAA ATCTACAATT ACTACTGCTG TATCCTCTGT AGAAATACCC
    AGAATGAAAG AGACAGGCAG TCCTTCATTA TCTATGACAC TTATGTATAA TAAAATGAAG
    GAAAGTATTT CTGTTGCCAC GTACAGTAGT CCAAATGGAA TAAGCACCAG TACGTTGTCT
    CCAAACACTG TGAGAGGAGA ACAAAACCGT TCCATGCAAA CTGCATTAGG TGATTTATCA
    GGCAATAGTT CTCCTGGACC ATCTCAAGGT GCAGCATATG AGCAGCATAA TTATCAGAGG
    CCAAAATACC ATATTTTTGG TAAGAGATCT TTAAACGAGC CTTTACCTAC TATAAAATCC
    ATTAGCCCAC TGGTTTCTGA TATAAAAAGA GAGGCAAAAC CCCCAAACTC TCATATACAT
    CACAATAAAT TCAATACCAG AAGACATGGT GGCACTCAAC AGCACAGAGC AAAACTGCAG
    CTTATGTACA GTGTCAAAAA TCCAATGGCC ACATTCTTAA ATGGTGTGGA GGGGTTCAGC
    CTCTCAAGGA ATCCTTGGAT GTCTGTTGGG CAAAGCACAG TAAATTCACA TCTTGCTTCA
    GTAGAAGATG CAGAAGATGC TCAATCAAGA AAGTTGTTAC CAAGCTTGTC TCCGCTCCAT
    AACAGACACT TGACTTCAAG AACCAAAATA AGCCGACAAC AACAGGCCTG TAAGTCTGAC
    CATGAATTTT GTGGATGGAA GCAAAGAAAG GACAATAAAC TCATCTGGAA TCTCATTTGG
    GGGGGTACAG ATCATCTTCA TGGAGGCCGT ACTAGTAAAG ACAAACGTAA AGGATTACTT
    TTATCTCTCA AGAATTTCTC CATGGAACCT ACCCAAGGGC AAAAGGCTTT GCTTGTTAGC
    CCTGTGTTAC ACCGTCCCAA CTGCGTGTCC TTCTGGTACA GACTCTTGCC AAACAGTACG
    GTAGGAACTC TGAACGTGTA TAGCCGACCA ACAAACATGT TAGGAACGCA GACTCTCCTG
    TGGACAAATC AAGGGCAGGA AAATCTGGGA TGGACTAAGG CACAAATAAC GTTTAAAAAG
    CAACATCAGC ATAATAACTT GCAGATAATC ATAAAAGGAG TCACGGGACC AGAAAGTGAT
    ATACGTATTA AGAATCTGTA TGTTGGCCAG TGTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755136.1
    CDS440..3358
    Translation

    Target ORF information:

    RefSeq Version XM_031899276.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis astacin-like metallo-endopeptidase (M12 family) (astl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGCTG TTTTGGCAAT ATGGCTGCTC GGAGCTGCAG GTGCGCTTGG GTTTCCAGTG 
    CCCAACCCGG CTCCGTCGGC AAGCTCTGAA GGAACAAACG CGAGCAATGC TGGCATCCTG
    GACATCAATG GGCCTCTCAT TCAAGGCGGT GCTTCAGGCC TGATGGAAGG GGACATTGTG
    AAAGAAAAGA GATCTATCCG CACATTCTCT GCTCGATTTG CAAAGTGGCC TAAGATAAAT
    GGCTCTGTGA TTATCCCCTA CACTCTGTCT TCTTCCTATG AAAGTTTTGA CCGGAATATA
    ATTCTAAAGG CCTTTCGGGA CTTGCAAGCA TCTACTTGCC TCCGCTTTGT GGAGCGCACT
    ACTGAACGGG ATTATATCGC CATAGAGCCA GCTATTGGGT GTTTTTCGAG TGTAGGGCGA
    GTTGGGGGGA TGCAGCTCGT CTCCCTGGCC TTTGAGTGCC TGAGGACAGA CAAGGGAAAG
    GGAATTGCTC TGCATGAATT GATGCACGTC GCTGGGTTTT GGCATGAACA TTCCAGAGCT
    GATCGAGATG ATTACATCTG GATAATTTGG GATGAGATTC TAATCGGCTA TGAGAAAAAC
    TTCTGTAAAT ATGCGACTAC CAACATGTTA GTCAAATATG AGCTGCAGTC CATCCTTCAT
    TACCCCAGAA GTGCATTCTC CAAGTCTGGG CAGGCCACAA TCAATCCAAA GTATCCATAT
    TCTAAGATTG AAATAGGTCA GAGAGAAAAA CTCAGTGCTT CTGACATCCT AAGGGTTAAC
    AAACTGTACT CCTGCTCTCA GTACACATCA TCAGATGATG ATGATGATGA ATTAAATTAT
    TTTCCACCAA TTGTTTATTC TTGCAACAAA GAACTCAAGC CTACATCCAC TAAATCATAT
    ATTGAACATA AGAATGGAGC TTTGCTAATG GAGACCCCAG CACAGATGGC CAATACCAAA
    CAAACATCCG TTTTAAGTCA AACAGCAGCT CCTACTGTGA GTTTTCAGAG AAGTTCAGTG
    AATGAAGGAA CTGCAATAGC CGGGCATAAA CATACAACTC TTTTAAGTAG TGTAAATATG
    ACTGGGGATT TTCAAGATAG TGTTTCTACA GGCTTTTACC AAACATCTTC TGAAACCAAT
    TCTTCTCAGT CATTAAATGA GCAATTGCAG GAAACACTAC AAAAGTCAAC AATGAGGTCT
    AATGAGGGTT TATTTACATC AGCCCCTACA ACACTGCCAT CTCATGTGAT TAATTTGAGC
    TCCCTCAATG CTTTTTCCAC CCTGGTAAAC CTGTCTGTGC TACCCACCAC CACCGTCACA
    CCTGAGCCTA TTAAAATACC TTCTAACAGT AATTTTTCTC AAATTGTAAC CAACATCATG
    CAAAAAACTC AACCTTCAGT TTCTACTTTG GCATCAAACT CTATATCACC TAACACTGTG
    GAACTCACTC ACAATGCTTT GATACCAACC AAAATCAGTC GCCACCAACA CAGAACAGAT
    GAAACCAGTA CCGTACCTGA GCAAGTGACT CTTCTCCATG TCAATGAAAG TACTGAACAT
    GGCACACCTA CAGAGTCCAA CCAAATTCTT ACTGAAAATG TTTCTTCCAA GCCAACTCCC
    TATCCCCCTG AGCACTTAGA CTACTCAAAC CTGACTAAAT CAGAAGGGGA AAATCTCAAT
    TATGTCATAA ACAGTTCGGA CACCTCTACA ACATCTATAA ATCTCGTTTC TGAAAGCATA
    TCCTCTCGGG CACCTCTGAA ATCTACAATT ACTACTGCTG TATCCTCTGT AGAAATACCC
    AGAATGAAAG AGACAGGCAG TCCTTCATTA TCTATGACAC TTATGTATAA TAAAATGAAG
    GAAAGTATTT CTGTTGCCAC GTACAGTAGT CCAAATGGAA TAAGCACCAG TACGTTGTCT
    CCAAACACTG TGAGAGGAGA ACAAAACCGT TCCATGCAAA CTGCATTAGG TGATTTATCA
    GGCAATAGTT CTCCTGGACC ATCTCAAGGT GCAGCATATG AGCAGCATAA TTATCAGAGG
    CCAAAATACC ATATTTTTGG TAAGAGATCT TTAAACGAGC CTTTACCTAC TATAAAATCC
    ATTAGCCCAC TGGTTTCTGA TATAAAAAGA GAGGCAAAAC CCCCAAACTC TCATATACAT
    CACAATAAAT TCAATACCAG AAGACATGGT GGCACTCAAC AGCACAGAGC AAAACTGCAG
    CTTATGTACA GTGTCAAAAA TCCAATGGCC ACATTCTTAA ATGGTGTGGA GGGGTTCAGC
    CTCTCAAGGA ATCCTTGGAT GTCTGTTGGG CAAAGCACAG TAAATTCACA TCTTGCTTCA
    GTAGAAGATG CAGAAGATGC TCAATCAAGA AAGTTGTTAC CAAGCTTGTC TCCGCTCCAT
    AACAGACACT TGACTTCAAG AACCAAAATA AGCCGACAAC AACAGGCCTG TAAGTCTGAC
    CATGAATTTT GTGGATGGAA GCAAAGAAAG GACAATAAAC TCATCTGGAA TCTCATTTGG
    GGGGGTACAG ATCATCTTCA TGGAGGCCGT ACTAGTAAAG ACAAACGTAA AGGATTACTT
    TTATCTCTCA AGAATTTCTC CATGGAACCT ACCCAAGGGC AAAAGGCTTT GCTTGTTAGC
    CCTGTGTTAC ACCGTCCCAA CTGCGTGTCC TTCTGGTACA GACTCTTGCC AAACAGTACG
    GTAGGAACTC TGAACGTGTA TAGCCGACCA ACAAACATGT TAGGAACGCA GACTCTCCTG
    TGGACAAATC AAGGGCAGGA AAATCTGGGA TGGACTAAGG CACAAATAAC GTTTAAAAAG
    CAACATCAGC ATAATAACTT GCAGATAATC ATAAAAGGAG TCACGGGACC AGAAAGTGAT
    ATACGTATTA AGAATCTGTA TGTTGGCCAG TGTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55292
    Clone ID Related Accession (Same CDS sequence) XM_031899277.1
    Accession Version XM_031899277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product astacin-like metalloendopeptidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGCAT CCTGGACATC AATGGGCAGA TCTATCCGCA CATTCTCTGC TCGATTTGCA 
    AAGTGGCCTA AGATAAATGG CTCTGTGATT ATCCCCTACA CTCTGTCTTC TTCCTATGAA
    AGTTTTGACC GGAATATAAT TCTAAAGGCC TTTCGGGACT TGCAAGCATC TACTTGCCTC
    CGCTTTGTGG AGCGCACTAC TGAACGGGAT TATATCGCCA TAGAGCCAGC TATTGGGTGT
    TTTTCGAGTG TAGGGCGAGT TGGGGGGATG CAGCTCGTCT CCCTGGCCTT TGAGTGCCTG
    AGGACAGACA AGGGAAAGGG AATTGCTCTG CATGAATTGA TGCACGTCGC TGGGTTTTGG
    CATGAACATT CCAGAGCTGA TCGAGATGAT TACATCTGGA TAATTTGGGA TGAGATTCTA
    ATCGGCTATG AGAAAAACTT CTGTAAATAT GCGACTACCA ACATGTTAGT CAAATATGAG
    CTGCAGTCCA TCCTTCATTA CCCCAGAAGT GCATTCTCCA AGTCTGGGCA GGCCACAATC
    AATCCAAAGT ATCCATATTC TAAGATTGAA ATAGGTCAGA GAGAAAAACT CAGTGCTTCT
    GACATCCTAA GGGTTAACAA ACTGTACTCC TGCTCTCAGT ACACATCATC AGATGATGAT
    GATGATGAAT TAAATTATTT TCCACCAATT GTTTATTCTT GCAACAAAGA ACTCAAGCCT
    ACATCCACTA AATCATATAT TGAACATAAG AATGGAGCTT TGCTAATGGA GACCCCAGCA
    CAGATGGCCA ATACCAAACA AACATCCGTT TTAAGTCAAA CAGCAGCTCC TACTGTGAGT
    TTTCAGAGAA GTTCAGTGAA TGAAGGAACT GCAATAGCCG GGCATAAACA TACAACTCTT
    TTAAGTAGTG TAAATATGAC TGGGGATTTT CAAGATAGTG TTTCTACAGG CTTTTACCAA
    ACATCTTCTG AAACCAATTC TTCTCAGTCA TTAAATGAGC AATTGCAGGA AACACTACAA
    AAGTCAACAA TGAGGTCTAA TGAGGGTTTA TTTACATCAG CCCCTACAAC ACTGCCATCT
    CATGTGATTA ATTTGAGCTC CCTCAATGCT TTTTCCACCC TGGTAAACCT GTCTGTGCTA
    CCCACCACCA CCGTCACACC TGAGCCTATT AAAATACCTT CTAACAGTAA TTTTTCTCAA
    ATTGTAACCA ACATCATGCA AAAAACTCAA CCTTCAGTTT CTACTTTGGC ATCAAACTCT
    ATATCACCTA ACACTGTGGA ACTCACTCAC AATGCTTTGA TACCAACCAA AATCAGTCGC
    CACCAACACA GAACAGATGA AACCAGTACC GTACCTGAGC AAGTGACTCT TCTCCATGTC
    AATGAAAGTA CTGAACATGG CACACCTACA GAGTCCAACC AAATTCTTAC TGAAAATGTT
    TCTTCCAAGC CAACTCCCTA TCCCCCTGAG CACTTAGACT ACTCAAACCT GACTAAATCA
    GAAGGGGAAA ATCTCAATTA TGTCATAAAC AGTTCGGACA CCTCTACAAC ATCTATAAAT
    CTCGTTTCTG AAAGCATATC CTCTCGGGCA CCTCTGAAAT CTACAATTAC TACTGCTGTA
    TCCTCTGTAG AAATACCCAG AATGAAAGAG ACAGGCAGTC CTTCATTATC TATGACACTT
    ATGTATAATA AAATGAAGGA AAGTATTTCT GTTGCCACGT ACAGTAGTCC AAATGGAATA
    AGCACCAGTA CGTTGTCTCC AAACACTGTG AGAGGAGAAC AAAACCGTTC CATGCAAACT
    GCATTAGGTG ATTTATCAGG CAATAGTTCT CCTGGACCAT CTCAAGGTGC AGCATATGAG
    CAGCATAATT ATCAGAGGCC AAAATACCAT ATTTTTGGTA AGAGATCTTT AAACGAGCCT
    TTACCTACTA TAAAATCCAT TAGCCCACTG GTTTCTGATA TAAAAAGAGA GGCAAAACCC
    CCAAACTCTC ATATACATCA CAATAAATTC AATACCAGAA GACATGGTGG CACTCAACAG
    CACAGAGCAA AACTGCAGCT TATGTACAGT GTCAAAAATC CAATGGCCAC ATTCTTAAAT
    GGTGTGGAGG GGTTCAGCCT CTCAAGGAAT CCTTGGATGT CTGTTGGGCA AAGCACAGTA
    AATTCACATC TTGCTTCAGT AGAAGATGCA GAAGATGCTC AATCAAGAAA GTTGTTACCA
    AGCTTGTCTC CGCTCCATAA CAGACACTTG ACTTCAAGAA CCAAAATAAG CCGACAACAA
    CAGGCCTGTA AGTCTGACCA TGAATTTTGT GGATGGAAGC AAAGAAAGGA CAATAAACTC
    ATCTGGAATC TCATTTGGGG GGGTACAGAT CATCTTCATG GAGGCCGTAC TAGTAAAGAC
    AAACGTAAAG GATTACTTTT ATCTCTCAAG AATTTCTCCA TGGAACCTAC CCAAGGGCAA
    AAGGCTTTGC TTGTTAGCCC TGTGTTACAC CGTCCCAACT GCGTGTCCTT CTGGTACAGA
    CTCTTGCCAA ACAGTACGGT AGGAACTCTG AACGTGTATA GCCGACCAAC AAACATGTTA
    GGAACGCAGA CTCTCCTGTG GACAAATCAA GGGCAGGAAA ATCTGGGATG GACTAAGGCA
    CAAATAACGT TTAAAAAGCA ACATCAGCAT AATAACTTGC AGATAATCAT AAAAGGAGTC
    ACGGGACCAG AAAGTGATAT ACGTATTAAG AATCTGTATG TTGGCCAGTG TGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755137.1
    CDS546..3302
    Translation

    Target ORF information:

    RefSeq Version XM_031899277.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis astacin-like metallo-endopeptidase (M12 family) (astl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGCAT CCTGGACATC AATGGGCAGA TCTATCCGCA CATTCTCTGC TCGATTTGCA 
    AAGTGGCCTA AGATAAATGG CTCTGTGATT ATCCCCTACA CTCTGTCTTC TTCCTATGAA
    AGTTTTGACC GGAATATAAT TCTAAAGGCC TTTCGGGACT TGCAAGCATC TACTTGCCTC
    CGCTTTGTGG AGCGCACTAC TGAACGGGAT TATATCGCCA TAGAGCCAGC TATTGGGTGT
    TTTTCGAGTG TAGGGCGAGT TGGGGGGATG CAGCTCGTCT CCCTGGCCTT TGAGTGCCTG
    AGGACAGACA AGGGAAAGGG AATTGCTCTG CATGAATTGA TGCACGTCGC TGGGTTTTGG
    CATGAACATT CCAGAGCTGA TCGAGATGAT TACATCTGGA TAATTTGGGA TGAGATTCTA
    ATCGGCTATG AGAAAAACTT CTGTAAATAT GCGACTACCA ACATGTTAGT CAAATATGAG
    CTGCAGTCCA TCCTTCATTA CCCCAGAAGT GCATTCTCCA AGTCTGGGCA GGCCACAATC
    AATCCAAAGT ATCCATATTC TAAGATTGAA ATAGGTCAGA GAGAAAAACT CAGTGCTTCT
    GACATCCTAA GGGTTAACAA ACTGTACTCC TGCTCTCAGT ACACATCATC AGATGATGAT
    GATGATGAAT TAAATTATTT TCCACCAATT GTTTATTCTT GCAACAAAGA ACTCAAGCCT
    ACATCCACTA AATCATATAT TGAACATAAG AATGGAGCTT TGCTAATGGA GACCCCAGCA
    CAGATGGCCA ATACCAAACA AACATCCGTT TTAAGTCAAA CAGCAGCTCC TACTGTGAGT
    TTTCAGAGAA GTTCAGTGAA TGAAGGAACT GCAATAGCCG GGCATAAACA TACAACTCTT
    TTAAGTAGTG TAAATATGAC TGGGGATTTT CAAGATAGTG TTTCTACAGG CTTTTACCAA
    ACATCTTCTG AAACCAATTC TTCTCAGTCA TTAAATGAGC AATTGCAGGA AACACTACAA
    AAGTCAACAA TGAGGTCTAA TGAGGGTTTA TTTACATCAG CCCCTACAAC ACTGCCATCT
    CATGTGATTA ATTTGAGCTC CCTCAATGCT TTTTCCACCC TGGTAAACCT GTCTGTGCTA
    CCCACCACCA CCGTCACACC TGAGCCTATT AAAATACCTT CTAACAGTAA TTTTTCTCAA
    ATTGTAACCA ACATCATGCA AAAAACTCAA CCTTCAGTTT CTACTTTGGC ATCAAACTCT
    ATATCACCTA ACACTGTGGA ACTCACTCAC AATGCTTTGA TACCAACCAA AATCAGTCGC
    CACCAACACA GAACAGATGA AACCAGTACC GTACCTGAGC AAGTGACTCT TCTCCATGTC
    AATGAAAGTA CTGAACATGG CACACCTACA GAGTCCAACC AAATTCTTAC TGAAAATGTT
    TCTTCCAAGC CAACTCCCTA TCCCCCTGAG CACTTAGACT ACTCAAACCT GACTAAATCA
    GAAGGGGAAA ATCTCAATTA TGTCATAAAC AGTTCGGACA CCTCTACAAC ATCTATAAAT
    CTCGTTTCTG AAAGCATATC CTCTCGGGCA CCTCTGAAAT CTACAATTAC TACTGCTGTA
    TCCTCTGTAG AAATACCCAG AATGAAAGAG ACAGGCAGTC CTTCATTATC TATGACACTT
    ATGTATAATA AAATGAAGGA AAGTATTTCT GTTGCCACGT ACAGTAGTCC AAATGGAATA
    AGCACCAGTA CGTTGTCTCC AAACACTGTG AGAGGAGAAC AAAACCGTTC CATGCAAACT
    GCATTAGGTG ATTTATCAGG CAATAGTTCT CCTGGACCAT CTCAAGGTGC AGCATATGAG
    CAGCATAATT ATCAGAGGCC AAAATACCAT ATTTTTGGTA AGAGATCTTT AAACGAGCCT
    TTACCTACTA TAAAATCCAT TAGCCCACTG GTTTCTGATA TAAAAAGAGA GGCAAAACCC
    CCAAACTCTC ATATACATCA CAATAAATTC AATACCAGAA GACATGGTGG CACTCAACAG
    CACAGAGCAA AACTGCAGCT TATGTACAGT GTCAAAAATC CAATGGCCAC ATTCTTAAAT
    GGTGTGGAGG GGTTCAGCCT CTCAAGGAAT CCTTGGATGT CTGTTGGGCA AAGCACAGTA
    AATTCACATC TTGCTTCAGT AGAAGATGCA GAAGATGCTC AATCAAGAAA GTTGTTACCA
    AGCTTGTCTC CGCTCCATAA CAGACACTTG ACTTCAAGAA CCAAAATAAG CCGACAACAA
    CAGGCCTGTA AGTCTGACCA TGAATTTTGT GGATGGAAGC AAAGAAAGGA CAATAAACTC
    ATCTGGAATC TCATTTGGGG GGGTACAGAT CATCTTCATG GAGGCCGTAC TAGTAAAGAC
    AAACGTAAAG GATTACTTTT ATCTCTCAAG AATTTCTCCA TGGAACCTAC CCAAGGGCAA
    AAGGCTTTGC TTGTTAGCCC TGTGTTACAC CGTCCCAACT GCGTGTCCTT CTGGTACAGA
    CTCTTGCCAA ACAGTACGGT AGGAACTCTG AACGTGTATA GCCGACCAAC AAACATGTTA
    GGAACGCAGA CTCTCCTGTG GACAAATCAA GGGCAGGAAA ATCTGGGATG GACTAAGGCA
    CAAATAACGT TTAAAAAGCA ACATCAGCAT AATAACTTGC AGATAATCAT AAAAGGAGTC
    ACGGGACCAG AAAGTGATAT ACGTATTAAG AATCTGTATG TTGGCCAGTG TGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.