ankle1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ankle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa48525 XM_002937942.5
Latest version!
Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa04288 XM_002937942.4
Latest version!
Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OXa42033 XM_018097004.1
Latest version!
Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa48525
    Clone ID Related Accession (Same CDS sequence) XM_002937942.5
    Accession Version XM_002937942.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat and LEM domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937942.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
    GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT
    GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA
    ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT
    GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTATTCT TGTTGAGGAA AGGTGGTAAC
    CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT
    CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA
    GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT
    CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT
    ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG
    GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG
    ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC
    ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG
    CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA
    GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT
    CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA
    ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT
    ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT
    TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA
    AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT
    GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG
    TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG
    GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT
    GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG
    CAACATCAGA ATCCTGTGCA CAACAGTCAA TGTCCTGGAG AGAGAGACTC ACAACTTCCA
    TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT
    GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA
    AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA
    AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT
    TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA
    GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT
    AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA
    TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG
    ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT
    TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA
    GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAAAT TATTAAGCAG
    CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC
    CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT
    CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC
    AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA
    ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA
    ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC
    ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT
    GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG
    TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA
    TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT
    GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA
    GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT
    GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT
    GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA
    CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT
    GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA
    CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT
    TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG
    TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT
    AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT
    GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA
    ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG
    AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA
    GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA
    TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT
    TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG
    AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA
    TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG
    CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT
    CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG
    GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG
    CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG
    CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937988.3
    CDS255..4397
    Translation

    Target ORF information:

    RefSeq Version XM_002937942.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937942.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
    GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT
    GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA
    ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT
    GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTATTCT TGTTGAGGAA AGGTGGTAAC
    CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT
    CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA
    GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT
    CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT
    ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG
    GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG
    ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC
    ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG
    CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA
    GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT
    CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA
    ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT
    ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT
    TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA
    AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT
    GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG
    TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG
    GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT
    GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG
    CAACATCAGA ATCCTGTGCA CAACAGTCAA TGTCCTGGAG AGAGAGACTC ACAACTTCCA
    TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT
    GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA
    AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA
    AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT
    TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA
    GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT
    AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA
    TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG
    ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT
    TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA
    GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAAAT TATTAAGCAG
    CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC
    CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT
    CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC
    AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA
    ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA
    ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC
    ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT
    GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG
    TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA
    TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT
    GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA
    GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT
    GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT
    GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA
    CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT
    GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA
    CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT
    TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG
    TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT
    AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT
    GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA
    ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG
    AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA
    GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA
    TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT
    TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG
    AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA
    TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG
    CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT
    CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG
    GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG
    CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG
    CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04288
    Clone ID Related Accession (Same CDS sequence) XM_002937942.4
    Accession Version XM_002937942.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat and LEM domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937942.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
    GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT
    GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA
    ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT
    GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC
    CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT
    CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA
    GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT
    CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT
    ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG
    GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG
    ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC
    ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG
    CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA
    GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT
    CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA
    ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT
    ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT
    TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA
    AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT
    GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG
    TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG
    GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT
    GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG
    CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA
    TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT
    GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA
    AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA
    AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT
    TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA
    GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT
    AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA
    TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG
    ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT
    TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA
    GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG
    CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC
    CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT
    CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC
    AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA
    ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA
    ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC
    ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT
    GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG
    TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA
    TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT
    GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA
    GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT
    GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT
    GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA
    CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT
    GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA
    CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT
    TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG
    TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT
    AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT
    GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA
    ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG
    AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA
    GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA
    TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT
    TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG
    AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA
    TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG
    CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT
    CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG
    GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG
    CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG
    CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937988.2
    CDS252..4394
    Translation

    Target ORF information:

    RefSeq Version XM_002937942.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937942.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
    GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT
    GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA
    ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT
    GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC
    CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT
    CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA
    GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT
    CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT
    ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG
    GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG
    ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC
    ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG
    CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA
    GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT
    CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA
    ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT
    ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT
    TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA
    AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT
    GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG
    TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG
    GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT
    GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG
    CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA
    TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT
    GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA
    AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA
    AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT
    TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA
    GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT
    AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA
    TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG
    ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT
    TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA
    GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG
    CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC
    CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT
    CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC
    AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA
    ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA
    ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC
    ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT
    GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG
    TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA
    TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT
    GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA
    GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT
    GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT
    GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA
    CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT
    GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA
    CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT
    TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG
    TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT
    AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT
    GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA
    ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG
    AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA
    GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA
    TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT
    TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG
    AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA
    TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG
    CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT
    CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG
    GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG
    CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG
    CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42033
    Clone ID Related Accession (Same CDS sequence) XM_018097004.1
    Accession Version XM_018097004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat and LEM domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
    GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT
    GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA
    ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT
    GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC
    CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT
    CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA
    GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT
    CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT
    ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG
    GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG
    ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC
    ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG
    CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA
    GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT
    CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA
    ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT
    ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT
    TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA
    AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT
    GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG
    TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG
    GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT
    GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG
    CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA
    TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT
    GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA
    AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA
    AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT
    TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA
    GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT
    AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA
    TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG
    ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT
    TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA
    GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG
    CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC
    CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT
    CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC
    AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA
    ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA
    ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC
    ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT
    GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG
    TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA
    TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT
    GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA
    GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT
    GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT
    GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA
    CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT
    GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA
    CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT
    TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG
    TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT
    AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT
    GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA
    ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG
    AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA
    GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA
    TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT
    TACTTGCTGC TAGACCCCAG TTTCGTCCAC AGATTCCCAC CTCGTTCATC TGGAAACACC
    CTGTCAATGA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952493.1
    CDS255..3989
    Translation

    Target ORF information:

    RefSeq Version XM_018097004.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
    GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT
    GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA
    ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT
    GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC
    CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT
    CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA
    GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT
    CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT
    ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG
    GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG
    ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC
    ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG
    CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA
    GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT
    CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA
    ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT
    ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT
    TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA
    AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT
    GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG
    TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG
    GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT
    GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG
    CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA
    TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT
    GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA
    AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA
    AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT
    TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA
    GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT
    AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA
    TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG
    ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT
    TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA
    GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG
    CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC
    CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT
    CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC
    AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA
    ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA
    ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC
    ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT
    GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG
    TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA
    TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT
    GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA
    GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT
    GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT
    GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA
    CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT
    GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA
    CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT
    TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG
    TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT
    AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT
    GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA
    ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG
    AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA
    GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA
    TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT
    TACTTGCTGC TAGACCCCAG TTTCGTCCAC AGATTCCCAC CTCGTTCATC TGGAAACACC
    CTGTCAATGA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.