col16a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col16a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col16a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa52324 XM_031896915.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa52325 XM_031896916.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa52326 XM_031896917.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa52327 XM_018091581.2
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa52328 XM_018091583.2
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa52329 XM_031896918.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa52330 XM_018091585.2
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa42757 XM_018091581.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa42758 XM_018091582.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa42759 XM_018091583.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa42760 XM_018091584.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OXa42761 XM_018091585.1
Latest version!
Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa52324
    Clone ID Related Accession (Same CDS sequence) XM_031896915.1
    Accession Version XM_031896915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGTGTG CCCGGACACA ATGGTATGCC AGGACCGCCT
    GGAGTGCCAG GAGAGCTGGG ACCCCTGGCT GCAGAAAGCA ACATTGATAT CCTGAAGAAT
    ATTTGTGGGG ACTGTGCCAG TGGGCAGAAC ACTCACCCAC CCCCCATCGC AATGAAAGGA
    CAGAAGGGAG AGCAAGGCCT ACCTGGGATT CCTGGAACAG AGGACTGTGA GAGGTGCCGA
    CCTGAAAAAG AGGATGAAGT GGGAGATGAG CCTGATCATG GCTGTGCAGG TGGGCCAGGG
    CAACCTGGGG CCCCAGGATT ACCAGGACAT GATGGACTAC CTGGTCCACG AGGACCCCCT
    GGACTTCCAG GAGCTTCGGG GCCCCCAGGT TTTCCAGGCA CTCTTGGCAC CCCCGGACTA
    CCTGGTATGC AGGGAGAAAG AGGACCACCA GGGCCACATG GAGAAAAAGG GGAATTGGGA
    CCACCTGGAC TTCCAGGTTA TCCTGGAACT ATGGGATCTC CAGGCCTTCC AGGTAGCAAA
    GGAGAGCGTG GCTACCATGG ACCTGCTGGA GAGAAAGGAG AGTCGGGTCC TCCTGGTATT
    CCGGGTGTTC CTGGTCCTGC TGGCCCTGCT GGACCAAGAG GTGAACGAGG AACTTCAGGG
    AGCTTCGGAG AAAAAGGTGA CCAGGGATTT TCAGGACCCC CAGGTTTCCC AGGACCTGCG
    GGACCTCCAG GTTTTCCTGG AAAGACAGGG TCTCCTGGAC CACCTGGGCC TACTGCTGAA
    AAGGGTTCAG AAGGATTACG TGGTTCTCCG GGACAACCTG GCTCATCAGG CCCACCAGGA
    CCTCCTGGAT TACAGGGGCC GCCTGGGATT GAAGGACTGG ACGGGAAGGA TGGGAAACCA
    GGACTGAGGG GAGAAATGGG ACCTCCTGGG CCTCCCGGGC ATAAGGGTAT TCCGGGATTC
    AAAGGGAAAA CAGGGCATCC TGGCTTGCCT GGGCAAAAGG GAGACTGTGG TCCTCCAGGT
    CCTTCAGGCA TTACTGGCAG GCCTGGTGCA GAGGGTGATC CTGGTCCAAT GGGTCCACCA
    GGCCGCCCAG GTCCTTCTGG AAACTTGGGA GCTGCAGGAC CTACAGGACC ACCAGGTAAT
    CCAGGCCCCC CTGGAATGGG ACTTAAAGGT GATCGAGGAC CACCAGGTGA TCGAGGACAA
    TTGGGTCTTC CGGGTGAGCC AGGTACCCCG GGACACCCAG GACCTGCTGG TGAAATGGGT
    TCAGATGGTC CTATGGGTAA GGAGGGTCCT TCTGGAAGGC CAGGAGCCCC TGGTTCTCCT
    GGAGAGAAGG GTGAAACCGG AATGCCTGGA TCTTCAGGCC CCCCAGGAGA AAGAGGAAGA
    CCAGGACCGC CAGGAACATC AGCAGCTTAT ACAAAAAGTG AATCATTAGG GATAGTTGGA
    CCAAGGGGAC CTCCAGGAGA GAGGGGCTCT TCTGGATCTC CAGGACCTGC TGGGGCACCA
    GGAAGCCCAG GACTTCCAGG AACACCTGCG GAATCCATCA GTTATGATGA GATTAAGAGA
    ATGATACGAC AAGAGTTGAT CAAGATCTTT GATGAGAGGA TGGCATATTA TACAACCCGC
    ATGCAGATGC CATTGGAGAT GGCTTCATCA CCAGGAAGAC CGGGCTCTCC TGGAAAGGAT
    GGATCCCCAG GACGTCCAGG ACCCCCTGGA TCACCTGGAC TGCCAGGACA AATGGGCCGT
    GAGGGGCGAC AAGGTGCCCC GGGAATGAGA GGAGAACCTG GATCAAAAGG AGAAAAGGGG
    GCAAATGGTA TTGGTGTCAT GGGAGATGTT GGACCTCCTG GCTCACCAGG ACCACAAGGG
    CCACCTGGAT ATGGCAAACT GGGATTACCA GGACCAATGG GACAGCAAGG TATACCAGGT
    ATTCCAGGTT CTCCAGGGGC AAAAGGTTAT CCAGGAAAAG ATGGTAGATG TAATCCCTCA
    GACTGCCACG GTATGATGGG CGTGCAAGAG TACCACCCAA AGAGCATGAA GGGTCCAGGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752775.1
    CDS166..5028
    Translation

    Target ORF information:

    RefSeq Version XM_031896915.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGTGTG CCCGGACACA ATGGTATGCC AGGACCGCCT
    GGAGTGCCAG GAGAGCTGGG ACCCCTGGCT GCAGAAAGCA ACATTGATAT CCTGAAGAAT
    ATTTGTGGGG ACTGTGCCAG TGGGCAGAAC ACTCACCCAC CCCCCATCGC AATGAAAGGA
    CAGAAGGGAG AGCAAGGCCT ACCTGGGATT CCTGGAACAG AGGACTGTGA GAGGTGCCGA
    CCTGAAAAAG AGGATGAAGT GGGAGATGAG CCTGATCATG GCTGTGCAGG TGGGCCAGGG
    CAACCTGGGG CCCCAGGATT ACCAGGACAT GATGGACTAC CTGGTCCACG AGGACCCCCT
    GGACTTCCAG GAGCTTCGGG GCCCCCAGGT TTTCCAGGCA CTCTTGGCAC CCCCGGACTA
    CCTGGTATGC AGGGAGAAAG AGGACCACCA GGGCCACATG GAGAAAAAGG GGAATTGGGA
    CCACCTGGAC TTCCAGGTTA TCCTGGAACT ATGGGATCTC CAGGCCTTCC AGGTAGCAAA
    GGAGAGCGTG GCTACCATGG ACCTGCTGGA GAGAAAGGAG AGTCGGGTCC TCCTGGTATT
    CCGGGTGTTC CTGGTCCTGC TGGCCCTGCT GGACCAAGAG GTGAACGAGG AACTTCAGGG
    AGCTTCGGAG AAAAAGGTGA CCAGGGATTT TCAGGACCCC CAGGTTTCCC AGGACCTGCG
    GGACCTCCAG GTTTTCCTGG AAAGACAGGG TCTCCTGGAC CACCTGGGCC TACTGCTGAA
    AAGGGTTCAG AAGGATTACG TGGTTCTCCG GGACAACCTG GCTCATCAGG CCCACCAGGA
    CCTCCTGGAT TACAGGGGCC GCCTGGGATT GAAGGACTGG ACGGGAAGGA TGGGAAACCA
    GGACTGAGGG GAGAAATGGG ACCTCCTGGG CCTCCCGGGC ATAAGGGTAT TCCGGGATTC
    AAAGGGAAAA CAGGGCATCC TGGCTTGCCT GGGCAAAAGG GAGACTGTGG TCCTCCAGGT
    CCTTCAGGCA TTACTGGCAG GCCTGGTGCA GAGGGTGATC CTGGTCCAAT GGGTCCACCA
    GGCCGCCCAG GTCCTTCTGG AAACTTGGGA GCTGCAGGAC CTACAGGACC ACCAGGTAAT
    CCAGGCCCCC CTGGAATGGG ACTTAAAGGT GATCGAGGAC CACCAGGTGA TCGAGGACAA
    TTGGGTCTTC CGGGTGAGCC AGGTACCCCG GGACACCCAG GACCTGCTGG TGAAATGGGT
    TCAGATGGTC CTATGGGTAA GGAGGGTCCT TCTGGAAGGC CAGGAGCCCC TGGTTCTCCT
    GGAGAGAAGG GTGAAACCGG AATGCCTGGA TCTTCAGGCC CCCCAGGAGA AAGAGGAAGA
    CCAGGACCGC CAGGAACATC AGCAGCTTAT ACAAAAAGTG AATCATTAGG GATAGTTGGA
    CCAAGGGGAC CTCCAGGAGA GAGGGGCTCT TCTGGATCTC CAGGACCTGC TGGGGCACCA
    GGAAGCCCAG GACTTCCAGG AACACCTGCG GAATCCATCA GTTATGATGA GATTAAGAGA
    ATGATACGAC AAGAGTTGAT CAAGATCTTT GATGAGAGGA TGGCATATTA TACAACCCGC
    ATGCAGATGC CATTGGAGAT GGCTTCATCA CCAGGAAGAC CGGGCTCTCC TGGAAAGGAT
    GGATCCCCAG GACGTCCAGG ACCCCCTGGA TCACCTGGAC TGCCAGGACA AATGGGCCGT
    GAGGGGCGAC AAGGTGCCCC GGGAATGAGA GGAGAACCTG GATCAAAAGG AGAAAAGGGG
    GCAAATGGTA TTGGTGTCAT GGGAGATGTT GGACCTCCTG GCTCACCAGG ACCACAAGGG
    CCACCTGGAT ATGGCAAACT GGGATTACCA GGACCAATGG GACAGCAAGG TATACCAGGT
    ATTCCAGGTT CTCCAGGGGC AAAAGGTTAT CCAGGAAAAG ATGGTAGATG TAATCCCTCA
    GACTGCCACG GTATGATGGG CGTGCAAGAG TACCACCCAA AGAGCATGAA GGGTCCAGGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52325
    Clone ID Related Accession (Same CDS sequence) XM_031896916.1
    Accession Version XM_031896916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGTGTG CCCGGACACA ATGGTATGCC AGGACCGCCT
    GGAGTGCCAG GAGAGCTGGG ACCCCTGGCT GCAGAAAGCA ACATTGATAT CCTGAAGAAT
    ATTTGTGGGG ACTGTGCCAG TGGGCAGAAC ACTCACCCAC CCCCCATCGC AATGAAAGGA
    CAGAAGGGAG AGCAAGGCCT ACCTGGGATT CCTGGAACAG AGGACTGTGA GAGGTGCCGA
    CCTGAAAAAG AGGATGAAGT GGGAGATGAG CCTGATCATG GCTGTGCAGG TGGGCCAGGG
    CAACCTGGGG CCCCAGGATT ACCAGGACAT GATGGACTAC CTGGTCCACG AGGACCCCCT
    GGACTTCCAG GAGCTTCGGG TATGCAGGGA GAAAGAGGAC CACCAGGGCC ACATGGAGAA
    AAAGGGGAAT TGGGACCACC TGGACTTCCA GGTTATCCTG GAACTATGGG ATCTCCAGGC
    CTTCCAGGTA GCAAAGGAGA GCGTGGCTAC CATGGACCTG CTGGAGAGAA AGGAGAGTCG
    GGTCCTCCTG GTATTCCGGG TGTTCCTGGT CCTGCTGGCC CTGCTGGACC AAGAGGTGAA
    CGAGGAACTT CAGGGAGCTT CGGAGAAAAA GGTGACCAGG GATTTTCAGG ACCCCCAGGT
    TTCCCAGGAC CTGCGGGACC TCCAGGTTTT CCTGGAAAGA CAGGGTCTCC TGGACCACCT
    GGGCCTACTG CTGAAAAGGG TTCAGAAGGA TTACGTGGTT CTCCGGGACA ACCTGGCTCA
    TCAGGCCCAC CAGGACCTCC TGGATTACAG GGGCCGCCTG GGATTGAAGG ACTGGACGGG
    AAGGATGGGA AACCAGGACT GAGGGGAGAA ATGGGACCTC CTGGGCCTCC CGGGCATAAG
    GGTATTCCGG GATTCAAAGG GAAAACAGGG CATCCTGGCT TGCCTGGGCA AAAGGGAGAC
    TGTGGTCCTC CAGGTCCTTC AGGCATTACT GGCAGGCCTG GTGCAGAGGG TGATCCTGGT
    CCAATGGGTC CACCAGGCCG CCCAGGTCCT TCTGGAAACT TGGGAGCTGC AGGACCTACA
    GGACCACCAG GTAATCCAGG CCCCCCTGGA ATGGGACTTA AAGGTGATCG AGGACCACCA
    GGTGATCGAG GACAATTGGG TCTTCCGGGT GAGCCAGGTA CCCCGGGACA CCCAGGACCT
    GCTGGTGAAA TGGGTTCAGA TGGTCCTATG GGTAAGGAGG GTCCTTCTGG AAGGCCAGGA
    GCCCCTGGTT CTCCTGGAGA GAAGGGTGAA ACCGGAATGC CTGGATCTTC AGGCCCCCCA
    GGAGAAAGAG GAAGACCAGG ACCGCCAGGA ACATCAGCAG CTTATACAAA AAGTGAATCA
    TTAGGGATAG TTGGACCAAG GGGACCTCCA GGAGAGAGGG GCTCTTCTGG ATCTCCAGGA
    CCTGCTGGGG CACCAGGAAG CCCAGGACTT CCAGGAACAC CTGCGGAATC CATCAGTTAT
    GATGAGATTA AGAGAATGAT ACGACAAGAG TTGATCAAGA TCTTTGATGA GAGGATGGCA
    TATTATACAA CCCGCATGCA GATGCCATTG GAGATGGCTT CATCACCAGG AAGACCGGGC
    TCTCCTGGAA AGGATGGATC CCCAGGACGT CCAGGACCCC CTGGATCACC TGGACTGCCA
    GGACAAATGG GCCGTGAGGG GCGACAAGGT GCCCCGGGAA TGAGAGGAGA ACCTGGATCA
    AAAGGAGAAA AGGGGGCAAA TGGTATTGGT GTCATGGGAG ATGTTGGACC TCCTGGCTCA
    CCAGGACCAC AAGGGCCACC TGGATATGGC AAACTGGGAT TACCAGGACC AATGGGACAG
    CAAGGTATAC CAGGTATTCC AGGTTCTCCA GGGGCAAAAG GTTATCCAGG AAAAGATGGT
    AGATGTAATC CCTCAGACTG CCACGGTATG ATGGGCGTGC AAGAGTACCA CCCAAAGAGC
    ATGAAGGGTC CAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752776.1
    CDS166..4983
    Translation

    Target ORF information:

    RefSeq Version XM_031896916.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGTGTG CCCGGACACA ATGGTATGCC AGGACCGCCT
    GGAGTGCCAG GAGAGCTGGG ACCCCTGGCT GCAGAAAGCA ACATTGATAT CCTGAAGAAT
    ATTTGTGGGG ACTGTGCCAG TGGGCAGAAC ACTCACCCAC CCCCCATCGC AATGAAAGGA
    CAGAAGGGAG AGCAAGGCCT ACCTGGGATT CCTGGAACAG AGGACTGTGA GAGGTGCCGA
    CCTGAAAAAG AGGATGAAGT GGGAGATGAG CCTGATCATG GCTGTGCAGG TGGGCCAGGG
    CAACCTGGGG CCCCAGGATT ACCAGGACAT GATGGACTAC CTGGTCCACG AGGACCCCCT
    GGACTTCCAG GAGCTTCGGG TATGCAGGGA GAAAGAGGAC CACCAGGGCC ACATGGAGAA
    AAAGGGGAAT TGGGACCACC TGGACTTCCA GGTTATCCTG GAACTATGGG ATCTCCAGGC
    CTTCCAGGTA GCAAAGGAGA GCGTGGCTAC CATGGACCTG CTGGAGAGAA AGGAGAGTCG
    GGTCCTCCTG GTATTCCGGG TGTTCCTGGT CCTGCTGGCC CTGCTGGACC AAGAGGTGAA
    CGAGGAACTT CAGGGAGCTT CGGAGAAAAA GGTGACCAGG GATTTTCAGG ACCCCCAGGT
    TTCCCAGGAC CTGCGGGACC TCCAGGTTTT CCTGGAAAGA CAGGGTCTCC TGGACCACCT
    GGGCCTACTG CTGAAAAGGG TTCAGAAGGA TTACGTGGTT CTCCGGGACA ACCTGGCTCA
    TCAGGCCCAC CAGGACCTCC TGGATTACAG GGGCCGCCTG GGATTGAAGG ACTGGACGGG
    AAGGATGGGA AACCAGGACT GAGGGGAGAA ATGGGACCTC CTGGGCCTCC CGGGCATAAG
    GGTATTCCGG GATTCAAAGG GAAAACAGGG CATCCTGGCT TGCCTGGGCA AAAGGGAGAC
    TGTGGTCCTC CAGGTCCTTC AGGCATTACT GGCAGGCCTG GTGCAGAGGG TGATCCTGGT
    CCAATGGGTC CACCAGGCCG CCCAGGTCCT TCTGGAAACT TGGGAGCTGC AGGACCTACA
    GGACCACCAG GTAATCCAGG CCCCCCTGGA ATGGGACTTA AAGGTGATCG AGGACCACCA
    GGTGATCGAG GACAATTGGG TCTTCCGGGT GAGCCAGGTA CCCCGGGACA CCCAGGACCT
    GCTGGTGAAA TGGGTTCAGA TGGTCCTATG GGTAAGGAGG GTCCTTCTGG AAGGCCAGGA
    GCCCCTGGTT CTCCTGGAGA GAAGGGTGAA ACCGGAATGC CTGGATCTTC AGGCCCCCCA
    GGAGAAAGAG GAAGACCAGG ACCGCCAGGA ACATCAGCAG CTTATACAAA AAGTGAATCA
    TTAGGGATAG TTGGACCAAG GGGACCTCCA GGAGAGAGGG GCTCTTCTGG ATCTCCAGGA
    CCTGCTGGGG CACCAGGAAG CCCAGGACTT CCAGGAACAC CTGCGGAATC CATCAGTTAT
    GATGAGATTA AGAGAATGAT ACGACAAGAG TTGATCAAGA TCTTTGATGA GAGGATGGCA
    TATTATACAA CCCGCATGCA GATGCCATTG GAGATGGCTT CATCACCAGG AAGACCGGGC
    TCTCCTGGAA AGGATGGATC CCCAGGACGT CCAGGACCCC CTGGATCACC TGGACTGCCA
    GGACAAATGG GCCGTGAGGG GCGACAAGGT GCCCCGGGAA TGAGAGGAGA ACCTGGATCA
    AAAGGAGAAA AGGGGGCAAA TGGTATTGGT GTCATGGGAG ATGTTGGACC TCCTGGCTCA
    CCAGGACCAC AAGGGCCACC TGGATATGGC AAACTGGGAT TACCAGGACC AATGGGACAG
    CAAGGTATAC CAGGTATTCC AGGTTCTCCA GGGGCAAAAG GTTATCCAGG AAAAGATGGT
    AGATGTAATC CCTCAGACTG CCACGGTATG ATGGGCGTGC AAGAGTACCA CCCAAAGAGC
    ATGAAGGGTC CAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52326
    Clone ID Related Accession (Same CDS sequence) XM_031896917.1
    Accession Version XM_031896917.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGGCCCTCC TGGGCCACCA
    GGGCCTCCAG GTCCCCCAGG ACTTCCTGGT CTACAGGGTG TGCCCGGACA CAATGGTATG
    CCAGGACCGC CTGGAGTGCC AGGAGAGCTG GGACCCCTGG CTGCAGAAAG CAACATTGAT
    ATCCTGAAGA ATATTTGTGG GGACTGTGCC AGTGGGCAGA ACACTCACCC ACCCCCCATC
    GCAATGAAAG GACAGAAGGG AGAGCAAGGC CTACCTGGGA TTCCTGGAAC AGAGGACTGT
    GAGAGGTGCC GACCTGAAAA AGAGGATGAA GTGGGAGATG AGCCTGATCA TGGCTGTGCA
    GGTGGGCCAG GGCAACCTGG GGCCCCAGGA TTACCAGGAC ATGATGGACT ACCTGGTCCA
    CGAGGACCCC CTGGACTTCC AGGAGCTTCG GGGCCCCCAG GTTTTCCAGG CACTCTTGGC
    ACCCCCGGAC TACCTGGTAT GCAGGGAGAA AGAGGACCAC CAGGGCCACA TGGAGAAAAA
    GGGGAATTGG GACCACCTGG ACTTCCAGGT TATCCTGGAA CTATGGGATC TCCAGGCCTT
    CCAGGTAGCA AAGGAGAGCG TGGCTACCAT GGACCTGCTG GAGAGAAAGG AGAGTCGGGT
    CCTCCTGGTA TTCCGGGTGT TCCTGGTCCT GCTGGCCCTG CTGGACCAAG AGGTGAACGA
    GGAACTTCAG GGAGCTTCGG AGAAAAAGGT GACCAGGGAT TTTCAGGACC CCCAGGTTTC
    CCAGGACCTG CGGGACCTCC AGGTTTTCCT GGAAAGACAG GGTCTCCTGG ACCACCTGGG
    CCTACTGCTG AAAAGGGTTC AGAAGGATTA CGTGGTTCTC CGGGACAACC TGGCTCATCA
    GGCCCACCAG GACCTCCTGG ATTACAGGGG CCGCCTGGGA TTGAAGGACT GGACGGGAAG
    GATGGGAAAC CAGGACTGAG GGGAGAAATG GGACCTCCTG GGCCTCCCGG GCATAAGGGT
    ATTCCGGGAT TCAAAGGGAA AACAGGGCAT CCTGGCTTGC CTGGGCAAAA GGGAGACTGT
    GGTCCTCCAG GTCCTTCAGG CATTACTGGC AGGCCTGGTG CAGAGGGTGA TCCTGGTCCA
    ATGGGTCCAC CAGGCCGCCC AGGTCCTTCT GGAAACTTGG GAGCTGCAGG ACCTACAGGA
    CCACCAGGTA ATCCAGGCCC CCCTGGAATG GGACTTAAAG GTGATCGAGG ACCACCAGGT
    GATCGAGGAC AATTGGGTCT TCCGGGTGAG CCAGGTACCC CGGGACACCC AGGACCTGCT
    GGTGAAATGG GTTCAGATGG TCCTATGGGT AAGGAGGGTC CTTCTGGAAG GCCAGGAGCC
    CCTGGTTCTC CTGGAGAGAA GGGTGAAACC GGAATGCCTG GATCTTCAGG CCCCCCAGGA
    GAAAGAGGAA GACCAGGACC GCCAGGAACA TCAGCAGCTT ATACAAAAAG TGAATCATTA
    GGGATAGTTG GACCAAGGGG ACCTCCAGGA GAGAGGGGCT CTTCTGGATC TCCAGGACCT
    GCTGGGGCAC CAGGAAGCCC AGGACTTCCA GGAACACCTG CGGAATCCAT CAGTTATGAT
    GAGATTAAGA GAATGATACG ACAAGAGTTG ATCAAGATCT TTGATGAGAG GATGGCATAT
    TATACAACCC GCATGCAGAT GCCATTGGAG ATGGCTTCAT CACCAGGAAG ACCGGGCTCT
    CCTGGAAAGG ATGGATCCCC AGGACGTCCA GGACCCCCTG GATCACCTGG ACTGCCAGGA
    CAAATGGGCC GTGAGGGGCG ACAAGGTGCC CCGGGAATGA GAGGAGAACC TGGATCAAAA
    GGAGAAAAGG GGGCAAATGG TATTGGTGTC ATGGGAGATG TTGGACCTCC TGGCTCACCA
    GGACCACAAG GGCCACCTGG ATATGGCAAA CTGGGATTAC CAGGACCAAT GGGACAGCAA
    GGTATACCAG GTATTCCAGG TTCTCCAGGG GCAAAAGGTT ATCCAGGAAA AGATGGTAGA
    TGTAATCCCT CAGACTGCCA CGGTATGATG GGCGTGCAAG AGTACCACCC AAAGAGCATG
    AAGGGTCCAG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752777.1
    CDS166..4980
    Translation

    Target ORF information:

    RefSeq Version XM_031896917.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGGCCCTCC TGGGCCACCA
    GGGCCTCCAG GTCCCCCAGG ACTTCCTGGT CTACAGGGTG TGCCCGGACA CAATGGTATG
    CCAGGACCGC CTGGAGTGCC AGGAGAGCTG GGACCCCTGG CTGCAGAAAG CAACATTGAT
    ATCCTGAAGA ATATTTGTGG GGACTGTGCC AGTGGGCAGA ACACTCACCC ACCCCCCATC
    GCAATGAAAG GACAGAAGGG AGAGCAAGGC CTACCTGGGA TTCCTGGAAC AGAGGACTGT
    GAGAGGTGCC GACCTGAAAA AGAGGATGAA GTGGGAGATG AGCCTGATCA TGGCTGTGCA
    GGTGGGCCAG GGCAACCTGG GGCCCCAGGA TTACCAGGAC ATGATGGACT ACCTGGTCCA
    CGAGGACCCC CTGGACTTCC AGGAGCTTCG GGGCCCCCAG GTTTTCCAGG CACTCTTGGC
    ACCCCCGGAC TACCTGGTAT GCAGGGAGAA AGAGGACCAC CAGGGCCACA TGGAGAAAAA
    GGGGAATTGG GACCACCTGG ACTTCCAGGT TATCCTGGAA CTATGGGATC TCCAGGCCTT
    CCAGGTAGCA AAGGAGAGCG TGGCTACCAT GGACCTGCTG GAGAGAAAGG AGAGTCGGGT
    CCTCCTGGTA TTCCGGGTGT TCCTGGTCCT GCTGGCCCTG CTGGACCAAG AGGTGAACGA
    GGAACTTCAG GGAGCTTCGG AGAAAAAGGT GACCAGGGAT TTTCAGGACC CCCAGGTTTC
    CCAGGACCTG CGGGACCTCC AGGTTTTCCT GGAAAGACAG GGTCTCCTGG ACCACCTGGG
    CCTACTGCTG AAAAGGGTTC AGAAGGATTA CGTGGTTCTC CGGGACAACC TGGCTCATCA
    GGCCCACCAG GACCTCCTGG ATTACAGGGG CCGCCTGGGA TTGAAGGACT GGACGGGAAG
    GATGGGAAAC CAGGACTGAG GGGAGAAATG GGACCTCCTG GGCCTCCCGG GCATAAGGGT
    ATTCCGGGAT TCAAAGGGAA AACAGGGCAT CCTGGCTTGC CTGGGCAAAA GGGAGACTGT
    GGTCCTCCAG GTCCTTCAGG CATTACTGGC AGGCCTGGTG CAGAGGGTGA TCCTGGTCCA
    ATGGGTCCAC CAGGCCGCCC AGGTCCTTCT GGAAACTTGG GAGCTGCAGG ACCTACAGGA
    CCACCAGGTA ATCCAGGCCC CCCTGGAATG GGACTTAAAG GTGATCGAGG ACCACCAGGT
    GATCGAGGAC AATTGGGTCT TCCGGGTGAG CCAGGTACCC CGGGACACCC AGGACCTGCT
    GGTGAAATGG GTTCAGATGG TCCTATGGGT AAGGAGGGTC CTTCTGGAAG GCCAGGAGCC
    CCTGGTTCTC CTGGAGAGAA GGGTGAAACC GGAATGCCTG GATCTTCAGG CCCCCCAGGA
    GAAAGAGGAA GACCAGGACC GCCAGGAACA TCAGCAGCTT ATACAAAAAG TGAATCATTA
    GGGATAGTTG GACCAAGGGG ACCTCCAGGA GAGAGGGGCT CTTCTGGATC TCCAGGACCT
    GCTGGGGCAC CAGGAAGCCC AGGACTTCCA GGAACACCTG CGGAATCCAT CAGTTATGAT
    GAGATTAAGA GAATGATACG ACAAGAGTTG ATCAAGATCT TTGATGAGAG GATGGCATAT
    TATACAACCC GCATGCAGAT GCCATTGGAG ATGGCTTCAT CACCAGGAAG ACCGGGCTCT
    CCTGGAAAGG ATGGATCCCC AGGACGTCCA GGACCCCCTG GATCACCTGG ACTGCCAGGA
    CAAATGGGCC GTGAGGGGCG ACAAGGTGCC CCGGGAATGA GAGGAGAACC TGGATCAAAA
    GGAGAAAAGG GGGCAAATGG TATTGGTGTC ATGGGAGATG TTGGACCTCC TGGCTCACCA
    GGACCACAAG GGCCACCTGG ATATGGCAAA CTGGGATTAC CAGGACCAAT GGGACAGCAA
    GGTATACCAG GTATTCCAGG TTCTCCAGGG GCAAAAGGTT ATCCAGGAAA AGATGGTAGA
    TGTAATCCCT CAGACTGCCA CGGTATGATG GGCGTGCAAG AGTACCACCC AAAGAGCATG
    AAGGGTCCAG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52327
    Clone ID Related Accession (Same CDS sequence) XM_018091581.2
    Accession Version XM_018091581.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGAATATTT GTGGGGACTG TGCCAGTGGG CAGAACACTC ACCCACCCCC CATCGCAATG
    AAAGGACAGA AGGGAGAGCA AGGCCTACCT GGGATTCCTG GAACAGAGGA CTGTGAGAGG
    TGCCGACCTG AAAAAGAGGA TGAAGTGGGA GATGAGCCTG ATCATGGCTG TGCAGGTGGG
    CCAGGGCAAC CTGGGGCCCC AGGATTACCA GGACATGATG GACTACCTGG TCCACGAGGA
    CCCCCTGGAC TTCCAGGAGC TTCGGGGCCC CCAGGTTTTC CAGGCACTCT TGGCACCCCC
    GGACTACCTG GTATGCAGGG AGAAAGAGGA CCACCAGGGC CACATGGAGA AAAAGGGGAA
    TTGGGACCAC CTGGACTTCC AGGTTATCCT GGAACTATGG GATCTCCAGG CCTTCCAGGT
    AGCAAAGGAG AGCGTGGCTA CCATGGACCT GCTGGAGAGA AAGGAGAGTC GGGTCCTCCT
    GGTATTCCGG GTGTTCCTGG TCCTGCTGGC CCTGCTGGAC CAAGAGGTGA ACGAGGAACT
    TCAGGGAGCT TCGGAGAAAA AGGTGACCAG GGATTTTCAG GACCCCCAGG TTTCCCAGGA
    CCTGCGGGAC CTCCAGGTTT TCCTGGAAAG ACAGGGTCTC CTGGACCACC TGGGCCTACT
    GCTGAAAAGG GTTCAGAAGG ATTACGTGGT TCTCCGGGAC AACCTGGCTC ATCAGGCCCA
    CCAGGACCTC CTGGATTACA GGGGCCGCCT GGGATTGAAG GACTGGACGG GAAGGATGGG
    AAACCAGGAC TGAGGGGAGA AATGGGACCT CCTGGGCCTC CCGGGCATAA GGGTATTCCG
    GGATTCAAAG GGAAAACAGG GCATCCTGGC TTGCCTGGGC AAAAGGGAGA CTGTGGTCCT
    CCAGGTCCTT CAGGCATTAC TGGCAGGCCT GGTGCAGAGG GTGATCCTGG TCCAATGGGT
    CCACCAGGCC GCCCAGGTCC TTCTGGAAAC TTGGGAGCTG CAGGACCTAC AGGACCACCA
    GGTAATCCAG GCCCCCCTGG AATGGGACTT AAAGGTGATC GAGGACCACC AGGTGATCGA
    GGACAATTGG GTCTTCCGGG TGAGCCAGGT ACCCCGGGAC ACCCAGGACC TGCTGGTGAA
    ATGGGTTCAG ATGGTCCTAT GGGTAAGGAG GGTCCTTCTG GAAGGCCAGG AGCCCCTGGT
    TCTCCTGGAG AGAAGGGTGA AACCGGAATG CCTGGATCTT CAGGCCCCCC AGGAGAAAGA
    GGAAGACCAG GACCGCCAGG AACATCAGCA GCTTATACAA AAAGTGAATC ATTAGGGATA
    GTTGGACCAA GGGGACCTCC AGGAGAGAGG GGCTCTTCTG GATCTCCAGG ACCTGCTGGG
    GCACCAGGAA GCCCAGGACT TCCAGGAACA CCTGCGGAAT CCATCAGTTA TGATGAGATT
    AAGAGAATGA TACGACAAGA GTTGATCAAG ATCTTTGATG AGAGGATGGC ATATTATACA
    ACCCGCATGC AGATGCCATT GGAGATGGCT TCATCACCAG GAAGACCGGG CTCTCCTGGA
    AAGGATGGAT CCCCAGGACG TCCAGGACCC CCTGGATCAC CTGGACTGCC AGGACAAATG
    GGCCGTGAGG GGCGACAAGG TGCCCCGGGA ATGAGAGGAG AACCTGGATC AAAAGGAGAA
    AAGGGGGCAA ATGGTATTGG TGTCATGGGA GATGTTGGAC CTCCTGGCTC ACCAGGACCA
    CAAGGGCCAC CTGGATATGG CAAACTGGGA TTACCAGGAC CAATGGGACA GCAAGGTATA
    CCAGGTATTC CAGGTTCTCC AGGGGCAAAA GGTTATCCAG GAAAAGATGG TAGATGTAAT
    CCCTCAGACT GCCACGGTAT GATGGGCGTG CAAGAGTACC ACCCAAAGAG CATGAAGGGT
    CCAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947070.2
    CDS166..4974
    Translation

    Target ORF information:

    RefSeq Version XM_018091581.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091581.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGAATATTT GTGGGGACTG TGCCAGTGGG CAGAACACTC ACCCACCCCC CATCGCAATG
    AAAGGACAGA AGGGAGAGCA AGGCCTACCT GGGATTCCTG GAACAGAGGA CTGTGAGAGG
    TGCCGACCTG AAAAAGAGGA TGAAGTGGGA GATGAGCCTG ATCATGGCTG TGCAGGTGGG
    CCAGGGCAAC CTGGGGCCCC AGGATTACCA GGACATGATG GACTACCTGG TCCACGAGGA
    CCCCCTGGAC TTCCAGGAGC TTCGGGGCCC CCAGGTTTTC CAGGCACTCT TGGCACCCCC
    GGACTACCTG GTATGCAGGG AGAAAGAGGA CCACCAGGGC CACATGGAGA AAAAGGGGAA
    TTGGGACCAC CTGGACTTCC AGGTTATCCT GGAACTATGG GATCTCCAGG CCTTCCAGGT
    AGCAAAGGAG AGCGTGGCTA CCATGGACCT GCTGGAGAGA AAGGAGAGTC GGGTCCTCCT
    GGTATTCCGG GTGTTCCTGG TCCTGCTGGC CCTGCTGGAC CAAGAGGTGA ACGAGGAACT
    TCAGGGAGCT TCGGAGAAAA AGGTGACCAG GGATTTTCAG GACCCCCAGG TTTCCCAGGA
    CCTGCGGGAC CTCCAGGTTT TCCTGGAAAG ACAGGGTCTC CTGGACCACC TGGGCCTACT
    GCTGAAAAGG GTTCAGAAGG ATTACGTGGT TCTCCGGGAC AACCTGGCTC ATCAGGCCCA
    CCAGGACCTC CTGGATTACA GGGGCCGCCT GGGATTGAAG GACTGGACGG GAAGGATGGG
    AAACCAGGAC TGAGGGGAGA AATGGGACCT CCTGGGCCTC CCGGGCATAA GGGTATTCCG
    GGATTCAAAG GGAAAACAGG GCATCCTGGC TTGCCTGGGC AAAAGGGAGA CTGTGGTCCT
    CCAGGTCCTT CAGGCATTAC TGGCAGGCCT GGTGCAGAGG GTGATCCTGG TCCAATGGGT
    CCACCAGGCC GCCCAGGTCC TTCTGGAAAC TTGGGAGCTG CAGGACCTAC AGGACCACCA
    GGTAATCCAG GCCCCCCTGG AATGGGACTT AAAGGTGATC GAGGACCACC AGGTGATCGA
    GGACAATTGG GTCTTCCGGG TGAGCCAGGT ACCCCGGGAC ACCCAGGACC TGCTGGTGAA
    ATGGGTTCAG ATGGTCCTAT GGGTAAGGAG GGTCCTTCTG GAAGGCCAGG AGCCCCTGGT
    TCTCCTGGAG AGAAGGGTGA AACCGGAATG CCTGGATCTT CAGGCCCCCC AGGAGAAAGA
    GGAAGACCAG GACCGCCAGG AACATCAGCA GCTTATACAA AAAGTGAATC ATTAGGGATA
    GTTGGACCAA GGGGACCTCC AGGAGAGAGG GGCTCTTCTG GATCTCCAGG ACCTGCTGGG
    GCACCAGGAA GCCCAGGACT TCCAGGAACA CCTGCGGAAT CCATCAGTTA TGATGAGATT
    AAGAGAATGA TACGACAAGA GTTGATCAAG ATCTTTGATG AGAGGATGGC ATATTATACA
    ACCCGCATGC AGATGCCATT GGAGATGGCT TCATCACCAG GAAGACCGGG CTCTCCTGGA
    AAGGATGGAT CCCCAGGACG TCCAGGACCC CCTGGATCAC CTGGACTGCC AGGACAAATG
    GGCCGTGAGG GGCGACAAGG TGCCCCGGGA ATGAGAGGAG AACCTGGATC AAAAGGAGAA
    AAGGGGGCAA ATGGTATTGG TGTCATGGGA GATGTTGGAC CTCCTGGCTC ACCAGGACCA
    CAAGGGCCAC CTGGATATGG CAAACTGGGA TTACCAGGAC CAATGGGACA GCAAGGTATA
    CCAGGTATTC CAGGTTCTCC AGGGGCAAAA GGTTATCCAG GAAAAGATGG TAGATGTAAT
    CCCTCAGACT GCCACGGTAT GATGGGCGTG CAAGAGTACC ACCCAAAGAG CATGAAGGGT
    CCAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52328
    Clone ID Related Accession (Same CDS sequence) XM_018091583.2
    Accession Version XM_018091583.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGGCCCTCC TGGGCCACCA
    GGGCCTCCAG GTCCCCCAGG ACTTCCTGGT CTACAGGGAC CCCTGGCTGC AGAAAGCAAC
    ATTGATATCC TGAAGAATAT TTGTGGGGAC TGTGCCAGTG GGCAGAACAC TCACCCACCC
    CCCATCGCAA TGAAAGGACA GAAGGGAGAG CAAGGCCTAC CTGGGATTCC TGGAACAGAG
    GACTGTGAGA GGTGCCGACC TGAAAAAGAG GATGAAGTGG GAGATGAGCC TGATCATGGC
    TGTGCAGGTG GGCCAGGGCA ACCTGGGGCC CCAGGATTAC CAGGACATGA TGGACTACCT
    GGTCCACGAG GACCCCCTGG ACTTCCAGGA GCTTCGGGGC CCCCAGGTTT TCCAGGCACT
    CTTGGCACCC CCGGACTACC TGGTATGCAG GGAGAAAGAG GACCACCAGG GCCACATGGA
    GAAAAAGGGG AATTGGGACC ACCTGGACTT CCAGGTTATC CTGGAACTAT GGGATCTCCA
    GGCCTTCCAG GTAGCAAAGG AGAGCGTGGC TACCATGGAC CTGCTGGAGA GAAAGGAGAG
    TCGGGTCCTC CTGGTATTCC GGGTGTTCCT GGTCCTGCTG GCCCTGCTGG ACCAAGAGGT
    GAACGAGGAA CTTCAGGGAG CTTCGGAGAA AAAGGTGACC AGGGATTTTC AGGACCCCCA
    GGTTTCCCAG GACCTGCGGG ACCTCCAGGT TTTCCTGGAA AGACAGGGTC TCCTGGACCA
    CCTGGGCCTA CTGCTGAAAA GGGTTCAGAA GGATTACGTG GTTCTCCGGG ACAACCTGGC
    TCATCAGGCC CACCAGGACC TCCTGGATTA CAGGGGCCGC CTGGGATTGA AGGACTGGAC
    GGGAAGGATG GGAAACCAGG ACTGAGGGGA GAAATGGGAC CTCCTGGGCC TCCCGGGCAT
    AAGGGTATTC CGGGATTCAA AGGGAAAACA GGGCATCCTG GCTTGCCTGG GCAAAAGGGA
    GACTGTGGTC CTCCAGGTCC TTCAGGCATT ACTGGCAGGC CTGGTGCAGA GGGTGATCCT
    GGTCCAATGG GTCCACCAGG CCGCCCAGGT CCTTCTGGAA ACTTGGGAGC TGCAGGACCT
    ACAGGACCAC CAGGTAATCC AGGCCCCCCT GGAATGGGAC TTAAAGGTGA TCGAGGACCA
    CCAGGTGATC GAGGACAATT GGGTCTTCCG GGTGAGCCAG GTACCCCGGG ACACCCAGGA
    CCTGCTGGTG AAATGGGTTC AGATGGTCCT ATGGGTAAGG AGGGTCCTTC TGGAAGGCCA
    GGAGCCCCTG GTTCTCCTGG AGAGAAGGGT GAAACCGGAA TGCCTGGATC TTCAGGCCCC
    CCAGGAGAAA GAGGAAGACC AGGACCGCCA GGAACATCAG CAGCTTATAC AAAAAGTGAA
    TCATTAGGGA TAGTTGGACC AAGGGGACCT CCAGGAGAGA GGGGCTCTTC TGGATCTCCA
    GGACCTGCTG GGGCACCAGG AAGCCCAGGA CTTCCAGGAA CACCTGCGGA ATCCATCAGT
    TATGATGAGA TTAAGAGAAT GATACGACAA GAGTTGATCA AGATCTTTGA TGAGAGGATG
    GCATATTATA CAACCCGCAT GCAGATGCCA TTGGAGATGG CTTCATCACC AGGAAGACCG
    GGCTCTCCTG GAAAGGATGG ATCCCCAGGA CGTCCAGGAC CCCCTGGATC ACCTGGACTG
    CCAGGACAAA TGGGCCGTGA GGGGCGACAA GGTGCCCCGG GAATGAGAGG AGAACCTGGA
    TCAAAAGGAG AAAAGGGGGC AAATGGTATT GGTGTCATGG GAGATGTTGG ACCTCCTGGC
    TCACCAGGAC CACAAGGGCC ACCTGGATAT GGCAAACTGG GATTACCAGG ACCAATGGGA
    CAGCAAGGTA TACCAGGTAT TCCAGGTTCT CCAGGGGCAA AAGGTTATCC AGGAAAAGAT
    GGTAGATGTA ATCCCTCAGA CTGCCACGGT ATGATGGGCG TGCAAGAGTA CCACCCAAAG
    AGCATGAAGG GTCCAGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947072.2
    CDS166..4926
    Translation

    Target ORF information:

    RefSeq Version XM_018091583.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091583.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGGCCCTCC TGGGCCACCA
    GGGCCTCCAG GTCCCCCAGG ACTTCCTGGT CTACAGGGAC CCCTGGCTGC AGAAAGCAAC
    ATTGATATCC TGAAGAATAT TTGTGGGGAC TGTGCCAGTG GGCAGAACAC TCACCCACCC
    CCCATCGCAA TGAAAGGACA GAAGGGAGAG CAAGGCCTAC CTGGGATTCC TGGAACAGAG
    GACTGTGAGA GGTGCCGACC TGAAAAAGAG GATGAAGTGG GAGATGAGCC TGATCATGGC
    TGTGCAGGTG GGCCAGGGCA ACCTGGGGCC CCAGGATTAC CAGGACATGA TGGACTACCT
    GGTCCACGAG GACCCCCTGG ACTTCCAGGA GCTTCGGGGC CCCCAGGTTT TCCAGGCACT
    CTTGGCACCC CCGGACTACC TGGTATGCAG GGAGAAAGAG GACCACCAGG GCCACATGGA
    GAAAAAGGGG AATTGGGACC ACCTGGACTT CCAGGTTATC CTGGAACTAT GGGATCTCCA
    GGCCTTCCAG GTAGCAAAGG AGAGCGTGGC TACCATGGAC CTGCTGGAGA GAAAGGAGAG
    TCGGGTCCTC CTGGTATTCC GGGTGTTCCT GGTCCTGCTG GCCCTGCTGG ACCAAGAGGT
    GAACGAGGAA CTTCAGGGAG CTTCGGAGAA AAAGGTGACC AGGGATTTTC AGGACCCCCA
    GGTTTCCCAG GACCTGCGGG ACCTCCAGGT TTTCCTGGAA AGACAGGGTC TCCTGGACCA
    CCTGGGCCTA CTGCTGAAAA GGGTTCAGAA GGATTACGTG GTTCTCCGGG ACAACCTGGC
    TCATCAGGCC CACCAGGACC TCCTGGATTA CAGGGGCCGC CTGGGATTGA AGGACTGGAC
    GGGAAGGATG GGAAACCAGG ACTGAGGGGA GAAATGGGAC CTCCTGGGCC TCCCGGGCAT
    AAGGGTATTC CGGGATTCAA AGGGAAAACA GGGCATCCTG GCTTGCCTGG GCAAAAGGGA
    GACTGTGGTC CTCCAGGTCC TTCAGGCATT ACTGGCAGGC CTGGTGCAGA GGGTGATCCT
    GGTCCAATGG GTCCACCAGG CCGCCCAGGT CCTTCTGGAA ACTTGGGAGC TGCAGGACCT
    ACAGGACCAC CAGGTAATCC AGGCCCCCCT GGAATGGGAC TTAAAGGTGA TCGAGGACCA
    CCAGGTGATC GAGGACAATT GGGTCTTCCG GGTGAGCCAG GTACCCCGGG ACACCCAGGA
    CCTGCTGGTG AAATGGGTTC AGATGGTCCT ATGGGTAAGG AGGGTCCTTC TGGAAGGCCA
    GGAGCCCCTG GTTCTCCTGG AGAGAAGGGT GAAACCGGAA TGCCTGGATC TTCAGGCCCC
    CCAGGAGAAA GAGGAAGACC AGGACCGCCA GGAACATCAG CAGCTTATAC AAAAAGTGAA
    TCATTAGGGA TAGTTGGACC AAGGGGACCT CCAGGAGAGA GGGGCTCTTC TGGATCTCCA
    GGACCTGCTG GGGCACCAGG AAGCCCAGGA CTTCCAGGAA CACCTGCGGA ATCCATCAGT
    TATGATGAGA TTAAGAGAAT GATACGACAA GAGTTGATCA AGATCTTTGA TGAGAGGATG
    GCATATTATA CAACCCGCAT GCAGATGCCA TTGGAGATGG CTTCATCACC AGGAAGACCG
    GGCTCTCCTG GAAAGGATGG ATCCCCAGGA CGTCCAGGAC CCCCTGGATC ACCTGGACTG
    CCAGGACAAA TGGGCCGTGA GGGGCGACAA GGTGCCCCGG GAATGAGAGG AGAACCTGGA
    TCAAAAGGAG AAAAGGGGGC AAATGGTATT GGTGTCATGG GAGATGTTGG ACCTCCTGGC
    TCACCAGGAC CACAAGGGCC ACCTGGATAT GGCAAACTGG GATTACCAGG ACCAATGGGA
    CAGCAAGGTA TACCAGGTAT TCCAGGTTCT CCAGGGGCAA AAGGTTATCC AGGAAAAGAT
    GGTAGATGTA ATCCCTCAGA CTGCCACGGT ATGATGGGCG TGCAAGAGTA CCACCCAAAG
    AGCATGAAGG GTCCAGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52329
    Clone ID Related Accession (Same CDS sequence) XM_031896918.1
    Accession Version XM_031896918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGTGTG CCCGGACACA ATGGTATGCC AGGACCGCCT
    GGAGTGCCAG GAGAGCTGGG ACCCCTGGCT GCAGAAAGCA ACATTGATAT CCTGAAGTGC
    CGACCTGAAA AAGAGGATGA AGTGGGAGAT GAGCCTGATC ATGGCTGTGC AGGTGGGCCA
    GGGCAACCTG GGGCCCCAGG ATTACCAGGA CATGATGGAC TACCTGGTCC ACGAGGACCC
    CCTGGACTTC CAGGAGCTTC GGGGCCCCCA GGTTTTCCAG GCACTCTTGG CACCCCCGGA
    CTACCTGGTA TGCAGGGAGA AAGAGGACCA CCAGGGCCAC ATGGAGAAAA AGGGGAATTG
    GGACCACCTG GACTTCCAGG TTATCCTGGA ACTATGGGAT CTCCAGGCCT TCCAGGTAGC
    AAAGGAGAGC GTGGCTACCA TGGACCTGCT GGAGAGAAAG GAGAGTCGGG TCCTCCTGGT
    ATTCCGGGTG TTCCTGGTCC TGCTGGCCCT GCTGGACCAA GAGGTGAACG AGGAACTTCA
    GGGAGCTTCG GAGAAAAAGG TGACCAGGGA TTTTCAGGAC CCCCAGGTTT CCCAGGACCT
    GCGGGACCTC CAGGTTTTCC TGGAAAGACA GGGTCTCCTG GACCACCTGG GCCTACTGCT
    GAAAAGGGTT CAGAAGGATT ACGTGGTTCT CCGGGACAAC CTGGCTCATC AGGCCCACCA
    GGACCTCCTG GATTACAGGG GCCGCCTGGG ATTGAAGGAC TGGACGGGAA GGATGGGAAA
    CCAGGACTGA GGGGAGAAAT GGGACCTCCT GGGCCTCCCG GGCATAAGGG TATTCCGGGA
    TTCAAAGGGA AAACAGGGCA TCCTGGCTTG CCTGGGCAAA AGGGAGACTG TGGTCCTCCA
    GGTCCTTCAG GCATTACTGG CAGGCCTGGT GCAGAGGGTG ATCCTGGTCC AATGGGTCCA
    CCAGGCCGCC CAGGTCCTTC TGGAAACTTG GGAGCTGCAG GACCTACAGG ACCACCAGGT
    AATCCAGGCC CCCCTGGAAT GGGACTTAAA GGTGATCGAG GACCACCAGG TGATCGAGGA
    CAATTGGGTC TTCCGGGTGA GCCAGGTACC CCGGGACACC CAGGACCTGC TGGTGAAATG
    GGTTCAGATG GTCCTATGGG TAAGGAGGGT CCTTCTGGAA GGCCAGGAGC CCCTGGTTCT
    CCTGGAGAGA AGGGTGAAAC CGGAATGCCT GGATCTTCAG GCCCCCCAGG AGAAAGAGGA
    AGACCAGGAC CGCCAGGAAC ATCAGCAGCT TATACAAAAA GTGAATCATT AGGGATAGTT
    GGACCAAGGG GACCTCCAGG AGAGAGGGGC TCTTCTGGAT CTCCAGGACC TGCTGGGGCA
    CCAGGAAGCC CAGGACTTCC AGGAACACCT GCGGAATCCA TCAGTTATGA TGAGATTAAG
    AGAATGATAC GACAAGAGTT GATCAAGATC TTTGATGAGA GGATGGCATA TTATACAACC
    CGCATGCAGA TGCCATTGGA GATGGCTTCA TCACCAGGAA GACCGGGCTC TCCTGGAAAG
    GATGGATCCC CAGGACGTCC AGGACCCCCT GGATCACCTG GACTGCCAGG ACAAATGGGC
    CGTGAGGGGC GACAAGGTGC CCCGGGAATG AGAGGAGAAC CTGGATCAAA AGGAGAAAAG
    GGGGCAAATG GTATTGGTGT CATGGGAGAT GTTGGACCTC CTGGCTCACC AGGACCACAA
    GGGCCACCTG GATATGGCAA ACTGGGATTA CCAGGACCAA TGGGACAGCA AGGTATACCA
    GGTATTCCAG GTTCTCCAGG GGCAAAAGGT TATCCAGGAA AAGATGGTAG ATGTAATCCC
    TCAGACTGCC ACGGTATGAT GGGCGTGCAA GAGTACCACC CAAAGAGCAT GAAGGGTCCA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752778.1
    CDS166..4911
    Translation

    Target ORF information:

    RefSeq Version XM_031896918.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGTGTG CCCGGACACA ATGGTATGCC AGGACCGCCT
    GGAGTGCCAG GAGAGCTGGG ACCCCTGGCT GCAGAAAGCA ACATTGATAT CCTGAAGTGC
    CGACCTGAAA AAGAGGATGA AGTGGGAGAT GAGCCTGATC ATGGCTGTGC AGGTGGGCCA
    GGGCAACCTG GGGCCCCAGG ATTACCAGGA CATGATGGAC TACCTGGTCC ACGAGGACCC
    CCTGGACTTC CAGGAGCTTC GGGGCCCCCA GGTTTTCCAG GCACTCTTGG CACCCCCGGA
    CTACCTGGTA TGCAGGGAGA AAGAGGACCA CCAGGGCCAC ATGGAGAAAA AGGGGAATTG
    GGACCACCTG GACTTCCAGG TTATCCTGGA ACTATGGGAT CTCCAGGCCT TCCAGGTAGC
    AAAGGAGAGC GTGGCTACCA TGGACCTGCT GGAGAGAAAG GAGAGTCGGG TCCTCCTGGT
    ATTCCGGGTG TTCCTGGTCC TGCTGGCCCT GCTGGACCAA GAGGTGAACG AGGAACTTCA
    GGGAGCTTCG GAGAAAAAGG TGACCAGGGA TTTTCAGGAC CCCCAGGTTT CCCAGGACCT
    GCGGGACCTC CAGGTTTTCC TGGAAAGACA GGGTCTCCTG GACCACCTGG GCCTACTGCT
    GAAAAGGGTT CAGAAGGATT ACGTGGTTCT CCGGGACAAC CTGGCTCATC AGGCCCACCA
    GGACCTCCTG GATTACAGGG GCCGCCTGGG ATTGAAGGAC TGGACGGGAA GGATGGGAAA
    CCAGGACTGA GGGGAGAAAT GGGACCTCCT GGGCCTCCCG GGCATAAGGG TATTCCGGGA
    TTCAAAGGGA AAACAGGGCA TCCTGGCTTG CCTGGGCAAA AGGGAGACTG TGGTCCTCCA
    GGTCCTTCAG GCATTACTGG CAGGCCTGGT GCAGAGGGTG ATCCTGGTCC AATGGGTCCA
    CCAGGCCGCC CAGGTCCTTC TGGAAACTTG GGAGCTGCAG GACCTACAGG ACCACCAGGT
    AATCCAGGCC CCCCTGGAAT GGGACTTAAA GGTGATCGAG GACCACCAGG TGATCGAGGA
    CAATTGGGTC TTCCGGGTGA GCCAGGTACC CCGGGACACC CAGGACCTGC TGGTGAAATG
    GGTTCAGATG GTCCTATGGG TAAGGAGGGT CCTTCTGGAA GGCCAGGAGC CCCTGGTTCT
    CCTGGAGAGA AGGGTGAAAC CGGAATGCCT GGATCTTCAG GCCCCCCAGG AGAAAGAGGA
    AGACCAGGAC CGCCAGGAAC ATCAGCAGCT TATACAAAAA GTGAATCATT AGGGATAGTT
    GGACCAAGGG GACCTCCAGG AGAGAGGGGC TCTTCTGGAT CTCCAGGACC TGCTGGGGCA
    CCAGGAAGCC CAGGACTTCC AGGAACACCT GCGGAATCCA TCAGTTATGA TGAGATTAAG
    AGAATGATAC GACAAGAGTT GATCAAGATC TTTGATGAGA GGATGGCATA TTATACAACC
    CGCATGCAGA TGCCATTGGA GATGGCTTCA TCACCAGGAA GACCGGGCTC TCCTGGAAAG
    GATGGATCCC CAGGACGTCC AGGACCCCCT GGATCACCTG GACTGCCAGG ACAAATGGGC
    CGTGAGGGGC GACAAGGTGC CCCGGGAATG AGAGGAGAAC CTGGATCAAA AGGAGAAAAG
    GGGGCAAATG GTATTGGTGT CATGGGAGAT GTTGGACCTC CTGGCTCACC AGGACCACAA
    GGGCCACCTG GATATGGCAA ACTGGGATTA CCAGGACCAA TGGGACAGCA AGGTATACCA
    GGTATTCCAG GTTCTCCAGG GGCAAAAGGT TATCCAGGAA AAGATGGTAG ATGTAATCCC
    TCAGACTGCC ACGGTATGAT GGGCGTGCAA GAGTACCACC CAAAGAGCAT GAAGGGTCCA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52330
    Clone ID Related Accession (Same CDS sequence) XM_018091585.2
    Accession Version XM_018091585.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGTGCCGAC CTGAAAAAGA GGATGAAGTG GGAGATGAGC CTGATCATGG CTGTGCAGGT
    GGGCCAGGGC AACCTGGGGC CCCAGGATTA CCAGGACATG ATGGACTACC TGGTCCACGA
    GGACCCCCTG GACTTCCAGG AGCTTCGGGG CCCCCAGGTT TTCCAGGCAC TCTTGGCACC
    CCCGGACTAC CTGGTATGCA GGGAGAAAGA GGACCACCAG GGCCACATGG AGAAAAAGGG
    GAATTGGGAC CACCTGGACT TCCAGGTTAT CCTGGAACTA TGGGATCTCC AGGCCTTCCA
    GGTAGCAAAG GAGAGCGTGG CTACCATGGA CCTGCTGGAG AGAAAGGAGA GTCGGGTCCT
    CCTGGTATTC CGGGTGTTCC TGGTCCTGCT GGCCCTGCTG GACCAAGAGG TGAACGAGGA
    ACTTCAGGGA GCTTCGGAGA AAAAGGTGAC CAGGGATTTT CAGGACCCCC AGGTTTCCCA
    GGACCTGCGG GACCTCCAGG TTTTCCTGGA AAGACAGGGT CTCCTGGACC ACCTGGGCCT
    ACTGCTGAAA AGGGTTCAGA AGGATTACGT GGTTCTCCGG GACAACCTGG CTCATCAGGC
    CCACCAGGAC CTCCTGGATT ACAGGGGCCG CCTGGGATTG AAGGACTGGA CGGGAAGGAT
    GGGAAACCAG GACTGAGGGG AGAAATGGGA CCTCCTGGGC CTCCCGGGCA TAAGGGTATT
    CCGGGATTCA AAGGGAAAAC AGGGCATCCT GGCTTGCCTG GGCAAAAGGG AGACTGTGGT
    CCTCCAGGTC CTTCAGGCAT TACTGGCAGG CCTGGTGCAG AGGGTGATCC TGGTCCAATG
    GGTCCACCAG GCCGCCCAGG TCCTTCTGGA AACTTGGGAG CTGCAGGACC TACAGGACCA
    CCAGGTAATC CAGGCCCCCC TGGAATGGGA CTTAAAGGTG ATCGAGGACC ACCAGGTGAT
    CGAGGACAAT TGGGTCTTCC GGGTGAGCCA GGTACCCCGG GACACCCAGG ACCTGCTGGT
    GAAATGGGTT CAGATGGTCC TATGGGTAAG GAGGGTCCTT CTGGAAGGCC AGGAGCCCCT
    GGTTCTCCTG GAGAGAAGGG TGAAACCGGA ATGCCTGGAT CTTCAGGCCC CCCAGGAGAA
    AGAGGAAGAC CAGGACCGCC AGGAACATCA GCAGCTTATA CAAAAAGTGA ATCATTAGGG
    ATAGTTGGAC CAAGGGGACC TCCAGGAGAG AGGGGCTCTT CTGGATCTCC AGGACCTGCT
    GGGGCACCAG GAAGCCCAGG ACTTCCAGGA ACACCTGCGG AATCCATCAG TTATGATGAG
    ATTAAGAGAA TGATACGACA AGAGTTGATC AAGATCTTTG ATGAGAGGAT GGCATATTAT
    ACAACCCGCA TGCAGATGCC ATTGGAGATG GCTTCATCAC CAGGAAGACC GGGCTCTCCT
    GGAAAGGATG GATCCCCAGG ACGTCCAGGA CCCCCTGGAT CACCTGGACT GCCAGGACAA
    ATGGGCCGTG AGGGGCGACA AGGTGCCCCG GGAATGAGAG GAGAACCTGG ATCAAAAGGA
    GAAAAGGGGG CAAATGGTAT TGGTGTCATG GGAGATGTTG GACCTCCTGG CTCACCAGGA
    CCACAAGGGC CACCTGGATA TGGCAAACTG GGATTACCAG GACCAATGGG ACAGCAAGGT
    ATACCAGGTA TTCCAGGTTC TCCAGGGGCA AAAGGTTATC CAGGAAAAGA TGGTAGATGT
    AATCCCTCAG ACTGCCACGG TATGATGGGC GTGCAAGAGT ACCACCCAAA GAGCATGAAG
    GGTCCAGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947074.2
    CDS166..4857
    Translation

    Target ORF information:

    RefSeq Version XM_018091585.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091585.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTACA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGCAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGTGCCGAC CTGAAAAAGA GGATGAAGTG GGAGATGAGC CTGATCATGG CTGTGCAGGT
    GGGCCAGGGC AACCTGGGGC CCCAGGATTA CCAGGACATG ATGGACTACC TGGTCCACGA
    GGACCCCCTG GACTTCCAGG AGCTTCGGGG CCCCCAGGTT TTCCAGGCAC TCTTGGCACC
    CCCGGACTAC CTGGTATGCA GGGAGAAAGA GGACCACCAG GGCCACATGG AGAAAAAGGG
    GAATTGGGAC CACCTGGACT TCCAGGTTAT CCTGGAACTA TGGGATCTCC AGGCCTTCCA
    GGTAGCAAAG GAGAGCGTGG CTACCATGGA CCTGCTGGAG AGAAAGGAGA GTCGGGTCCT
    CCTGGTATTC CGGGTGTTCC TGGTCCTGCT GGCCCTGCTG GACCAAGAGG TGAACGAGGA
    ACTTCAGGGA GCTTCGGAGA AAAAGGTGAC CAGGGATTTT CAGGACCCCC AGGTTTCCCA
    GGACCTGCGG GACCTCCAGG TTTTCCTGGA AAGACAGGGT CTCCTGGACC ACCTGGGCCT
    ACTGCTGAAA AGGGTTCAGA AGGATTACGT GGTTCTCCGG GACAACCTGG CTCATCAGGC
    CCACCAGGAC CTCCTGGATT ACAGGGGCCG CCTGGGATTG AAGGACTGGA CGGGAAGGAT
    GGGAAACCAG GACTGAGGGG AGAAATGGGA CCTCCTGGGC CTCCCGGGCA TAAGGGTATT
    CCGGGATTCA AAGGGAAAAC AGGGCATCCT GGCTTGCCTG GGCAAAAGGG AGACTGTGGT
    CCTCCAGGTC CTTCAGGCAT TACTGGCAGG CCTGGTGCAG AGGGTGATCC TGGTCCAATG
    GGTCCACCAG GCCGCCCAGG TCCTTCTGGA AACTTGGGAG CTGCAGGACC TACAGGACCA
    CCAGGTAATC CAGGCCCCCC TGGAATGGGA CTTAAAGGTG ATCGAGGACC ACCAGGTGAT
    CGAGGACAAT TGGGTCTTCC GGGTGAGCCA GGTACCCCGG GACACCCAGG ACCTGCTGGT
    GAAATGGGTT CAGATGGTCC TATGGGTAAG GAGGGTCCTT CTGGAAGGCC AGGAGCCCCT
    GGTTCTCCTG GAGAGAAGGG TGAAACCGGA ATGCCTGGAT CTTCAGGCCC CCCAGGAGAA
    AGAGGAAGAC CAGGACCGCC AGGAACATCA GCAGCTTATA CAAAAAGTGA ATCATTAGGG
    ATAGTTGGAC CAAGGGGACC TCCAGGAGAG AGGGGCTCTT CTGGATCTCC AGGACCTGCT
    GGGGCACCAG GAAGCCCAGG ACTTCCAGGA ACACCTGCGG AATCCATCAG TTATGATGAG
    ATTAAGAGAA TGATACGACA AGAGTTGATC AAGATCTTTG ATGAGAGGAT GGCATATTAT
    ACAACCCGCA TGCAGATGCC ATTGGAGATG GCTTCATCAC CAGGAAGACC GGGCTCTCCT
    GGAAAGGATG GATCCCCAGG ACGTCCAGGA CCCCCTGGAT CACCTGGACT GCCAGGACAA
    ATGGGCCGTG AGGGGCGACA AGGTGCCCCG GGAATGAGAG GAGAACCTGG ATCAAAAGGA
    GAAAAGGGGG CAAATGGTAT TGGTGTCATG GGAGATGTTG GACCTCCTGG CTCACCAGGA
    CCACAAGGGC CACCTGGATA TGGCAAACTG GGATTACCAG GACCAATGGG ACAGCAAGGT
    ATACCAGGTA TTCCAGGTTC TCCAGGGGCA AAAGGTTATC CAGGAAAAGA TGGTAGATGT
    AATCCCTCAG ACTGCCACGG TATGATGGGC GTGCAAGAGT ACCACCCAAA GAGCATGAAG
    GGTCCAGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42757
    Clone ID Related Accession (Same CDS sequence) XM_018091581.1
    Accession Version XM_018091581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGAATATTT GTGGGGACTG TGCCAGTGGG CAGAACACTC ACCCACCCCC CATCGCAATG
    AAAGGACAGA AGGGAGAGCA AGGCCTACCT GGGATTCCTG GAACAGAGGA CTGTGAGAGG
    TGCCGACCTG AAAAAGAGGA TGAAGTGGGA GATGAGCCTG ATCATGGCTG TGCAGGTGGG
    CCAGGGCAAC CTGGGGCCCC AGGATTACCA GGACATGATG GACTACCTGG TCCACGAGGA
    CCCCCTGGAC TTCCAGGAGC TTCGGGGCCC CCAGGTTTTC CAGGCACTCT TGGCACCCCC
    GGACTACCTG GTATGCAGGG AGAAAGAGGA CCACCAGGGC CACATGGAGA AAAAGGGGAA
    TTGGGACCAC CTGGACTTCC AGGTTATCCT GGAACTATGG GATCTCCAGG CCTTCCAGGT
    AGCAAAGGAG AGCGTGGCTA CCATGGACCT GCTGGAGAGA AAGGAGAGTC GGGTCCTCCT
    GGTATTCCGG GTGTTCCTGG TCCTGCTGGC CCTGCTGGAC CAAGAGGTGA ACGAGGAACT
    TCAGGGAGCT TCGGAGAAAA AGGTGACCAG GGATTTTCAG GCCCCCCAGG TTTCCCAGGA
    CCTGCGGGAC CTCCAGGTTT TCCTGGAAAG ACAGGGTCTC CTGGACCACC TGGGCCTACT
    GCTGAAAAGG GTTCAGAAGG ATTACGTGGT TCTCCGGGAC AACCTGGCTC ATCAGGCCCA
    CCAGGACCTC CTGGATTACA GGGGCCGCCT GGGATTGAAG GACTGGACGG GAAGGATGGG
    AAACCAGGAC TGAGGGGAGA AATGGGACCT CCTGGGCCTC CTGGGCATAA GGGTATTCCG
    GGATTCAAAG GGAAAACAGG GCATCCTGGC TTGCCTGGGC AAAAGGGAGA CTGTGGTCCT
    CCAGGTCCTT CAGGCATTAC TGGCAGGCCT GGTGCAGAGG GTGATCCTGG TCCAATGGGT
    CCACCAGGCC GCCCAGGTCC TTCTGGAAAC TTGGGAGCTG CAGGACCTAC AGGACCACCA
    GGTAATCCAG GCCCCCCTGG AATGGGACTT AAAGGTGATC GAGGACCACC AGGTGATCGA
    GGACAATTGG GTCTTCCGGG TGAGCCAGGT ACCCCGGGAC ACCCAGGACC TGCTGGTGAA
    ATGGGTTCAG ATGGTCCTAT GGGTAAGGAG GGTCCTTCTG GAAGGCCAGG AGCCCCTGGT
    TCTCCTGGAG AGAAGGGTGA AACCGGAATG CCTGGATCTT CAGGCCCCCC AGGAGAAAGA
    GGAAGACCAG GACCGCCAGG AACATCAGCA GCTTATACAA AAAGTGAATC ATTAGGGATA
    GTTGGACCAA GGGGACCTCC AGGAGAGAGG GGCTCTTCTG GATCTCCAGG ACCTGCTGGG
    GCACCAGGAA GCCCAGGACT TCCAGGAACA CCTGCGGAAT CCATCAGTTA TGATGAGATT
    AAGAGAATGA TACGACAAGA GTTGATCAAG ATCTTTGATG AGAGGATGGC ATATTATACA
    ACCCGCATGC AGATGCCATT GGAGATGGCT TCATCACCAG GAAGACCGGG CTCTCCTGGA
    AAGGATGGAT CCCCAGGACG TCCAGGACCC CCTGGATCAC CTGGACTGCC AGGACAAATG
    GGCCGTGAGG GGCGACAAGG TGCCCCGGGA ATGAGAGGAG AACCTGGATC AAAAGGAGAA
    AAGGGGGCAA ATGGTATTGG TGTCATGGGA GATGTTGGAC CTCCTGGCTC ACCAGGACCA
    CAAGGGCCAC CTGGATATGG CAAACTGGGA TTACCAGGAC CAATGGGACA GCAAGGTATA
    CCAGGTATTC CAGGTTCTCC AGGGGCAAAA GGTTATCCAG GAAAAGATGG TAGATGTAAT
    CCCTCAGACT GCCACGGTAT GATGGGCGTG CAAGAGTACC ACCCAAAGAG CATGAAGGGT
    CCAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947070.1
    CDS168..4976
    Translation

    Target ORF information:

    RefSeq Version XM_018091581.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGAATATTT GTGGGGACTG TGCCAGTGGG CAGAACACTC ACCCACCCCC CATCGCAATG
    AAAGGACAGA AGGGAGAGCA AGGCCTACCT GGGATTCCTG GAACAGAGGA CTGTGAGAGG
    TGCCGACCTG AAAAAGAGGA TGAAGTGGGA GATGAGCCTG ATCATGGCTG TGCAGGTGGG
    CCAGGGCAAC CTGGGGCCCC AGGATTACCA GGACATGATG GACTACCTGG TCCACGAGGA
    CCCCCTGGAC TTCCAGGAGC TTCGGGGCCC CCAGGTTTTC CAGGCACTCT TGGCACCCCC
    GGACTACCTG GTATGCAGGG AGAAAGAGGA CCACCAGGGC CACATGGAGA AAAAGGGGAA
    TTGGGACCAC CTGGACTTCC AGGTTATCCT GGAACTATGG GATCTCCAGG CCTTCCAGGT
    AGCAAAGGAG AGCGTGGCTA CCATGGACCT GCTGGAGAGA AAGGAGAGTC GGGTCCTCCT
    GGTATTCCGG GTGTTCCTGG TCCTGCTGGC CCTGCTGGAC CAAGAGGTGA ACGAGGAACT
    TCAGGGAGCT TCGGAGAAAA AGGTGACCAG GGATTTTCAG GCCCCCCAGG TTTCCCAGGA
    CCTGCGGGAC CTCCAGGTTT TCCTGGAAAG ACAGGGTCTC CTGGACCACC TGGGCCTACT
    GCTGAAAAGG GTTCAGAAGG ATTACGTGGT TCTCCGGGAC AACCTGGCTC ATCAGGCCCA
    CCAGGACCTC CTGGATTACA GGGGCCGCCT GGGATTGAAG GACTGGACGG GAAGGATGGG
    AAACCAGGAC TGAGGGGAGA AATGGGACCT CCTGGGCCTC CTGGGCATAA GGGTATTCCG
    GGATTCAAAG GGAAAACAGG GCATCCTGGC TTGCCTGGGC AAAAGGGAGA CTGTGGTCCT
    CCAGGTCCTT CAGGCATTAC TGGCAGGCCT GGTGCAGAGG GTGATCCTGG TCCAATGGGT
    CCACCAGGCC GCCCAGGTCC TTCTGGAAAC TTGGGAGCTG CAGGACCTAC AGGACCACCA
    GGTAATCCAG GCCCCCCTGG AATGGGACTT AAAGGTGATC GAGGACCACC AGGTGATCGA
    GGACAATTGG GTCTTCCGGG TGAGCCAGGT ACCCCGGGAC ACCCAGGACC TGCTGGTGAA
    ATGGGTTCAG ATGGTCCTAT GGGTAAGGAG GGTCCTTCTG GAAGGCCAGG AGCCCCTGGT
    TCTCCTGGAG AGAAGGGTGA AACCGGAATG CCTGGATCTT CAGGCCCCCC AGGAGAAAGA
    GGAAGACCAG GACCGCCAGG AACATCAGCA GCTTATACAA AAAGTGAATC ATTAGGGATA
    GTTGGACCAA GGGGACCTCC AGGAGAGAGG GGCTCTTCTG GATCTCCAGG ACCTGCTGGG
    GCACCAGGAA GCCCAGGACT TCCAGGAACA CCTGCGGAAT CCATCAGTTA TGATGAGATT
    AAGAGAATGA TACGACAAGA GTTGATCAAG ATCTTTGATG AGAGGATGGC ATATTATACA
    ACCCGCATGC AGATGCCATT GGAGATGGCT TCATCACCAG GAAGACCGGG CTCTCCTGGA
    AAGGATGGAT CCCCAGGACG TCCAGGACCC CCTGGATCAC CTGGACTGCC AGGACAAATG
    GGCCGTGAGG GGCGACAAGG TGCCCCGGGA ATGAGAGGAG AACCTGGATC AAAAGGAGAA
    AAGGGGGCAA ATGGTATTGG TGTCATGGGA GATGTTGGAC CTCCTGGCTC ACCAGGACCA
    CAAGGGCCAC CTGGATATGG CAAACTGGGA TTACCAGGAC CAATGGGACA GCAAGGTATA
    CCAGGTATTC CAGGTTCTCC AGGGGCAAAA GGTTATCCAG GAAAAGATGG TAGATGTAAT
    CCCTCAGACT GCCACGGTAT GATGGGCGTG CAAGAGTACC ACCCAAAGAG CATGAAGGGT
    CCAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42758
    Clone ID Related Accession (Same CDS sequence) XM_018091582.1
    Accession Version XM_018091582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGAATATTT GTGGGGACTG TGCCAGTGGG CAGAACACTC ACCCACCCCC CATCGCAATG
    AAAGGACAGA AGGGAGAGCA AGGCCTACCT GGGATTCCTG GAACAGAGGA CTGTGAGAGG
    TGCCGACCTG AAAAAGAGGA TGAAGTGGGA GATGAGCCTG ATCATGGCTG TGCAGGTGGG
    CCAGGGCAAC CTGGGGCCCC AGGATTACCA GGACATGATG GACTACCTGG TCCACGAGGA
    CCCCCTGGAC TTCCAGGAGC TTCGGGTATG CAGGGAGAAA GAGGACCACC AGGGCCACAT
    GGAGAAAAAG GGGAATTGGG ACCACCTGGA CTTCCAGGTT ATCCTGGAAC TATGGGATCT
    CCAGGCCTTC CAGGTAGCAA AGGAGAGCGT GGCTACCATG GACCTGCTGG AGAGAAAGGA
    GAGTCGGGTC CTCCTGGTAT TCCGGGTGTT CCTGGTCCTG CTGGCCCTGC TGGACCAAGA
    GGTGAACGAG GAACTTCAGG GAGCTTCGGA GAAAAAGGTG ACCAGGGATT TTCAGGCCCC
    CCAGGTTTCC CAGGACCTGC GGGACCTCCA GGTTTTCCTG GAAAGACAGG GTCTCCTGGA
    CCACCTGGGC CTACTGCTGA AAAGGGTTCA GAAGGATTAC GTGGTTCTCC GGGACAACCT
    GGCTCATCAG GCCCACCAGG ACCTCCTGGA TTACAGGGGC CGCCTGGGAT TGAAGGACTG
    GACGGGAAGG ATGGGAAACC AGGACTGAGG GGAGAAATGG GACCTCCTGG GCCTCCTGGG
    CATAAGGGTA TTCCGGGATT CAAAGGGAAA ACAGGGCATC CTGGCTTGCC TGGGCAAAAG
    GGAGACTGTG GTCCTCCAGG TCCTTCAGGC ATTACTGGCA GGCCTGGTGC AGAGGGTGAT
    CCTGGTCCAA TGGGTCCACC AGGCCGCCCA GGTCCTTCTG GAAACTTGGG AGCTGCAGGA
    CCTACAGGAC CACCAGGTAA TCCAGGCCCC CCTGGAATGG GACTTAAAGG TGATCGAGGA
    CCACCAGGTG ATCGAGGACA ATTGGGTCTT CCGGGTGAGC CAGGTACCCC GGGACACCCA
    GGACCTGCTG GTGAAATGGG TTCAGATGGT CCTATGGGTA AGGAGGGTCC TTCTGGAAGG
    CCAGGAGCCC CTGGTTCTCC TGGAGAGAAG GGTGAAACCG GAATGCCTGG ATCTTCAGGC
    CCCCCAGGAG AAAGAGGAAG ACCAGGACCG CCAGGAACAT CAGCAGCTTA TACAAAAAGT
    GAATCATTAG GGATAGTTGG ACCAAGGGGA CCTCCAGGAG AGAGGGGCTC TTCTGGATCT
    CCAGGACCTG CTGGGGCACC AGGAAGCCCA GGACTTCCAG GAACACCTGC GGAATCCATC
    AGTTATGATG AGATTAAGAG AATGATACGA CAAGAGTTGA TCAAGATCTT TGATGAGAGG
    ATGGCATATT ATACAACCCG CATGCAGATG CCATTGGAGA TGGCTTCATC ACCAGGAAGA
    CCGGGCTCTC CTGGAAAGGA TGGATCCCCA GGACGTCCAG GACCCCCTGG ATCACCTGGA
    CTGCCAGGAC AAATGGGCCG TGAGGGGCGA CAAGGTGCCC CGGGAATGAG AGGAGAACCT
    GGATCAAAAG GAGAAAAGGG GGCAAATGGT ATTGGTGTCA TGGGAGATGT TGGACCTCCT
    GGCTCACCAG GACCACAAGG GCCACCTGGA TATGGCAAAC TGGGATTACC AGGACCAATG
    GGACAGCAAG GTATACCAGG TATTCCAGGT TCTCCAGGGG CAAAAGGTTA TCCAGGAAAA
    GATGGTAGAT GTAATCCCTC AGACTGCCAC GGTATGATGG GCGTGCAAGA GTACCACCCA
    AAGAGCATGA AGGGTCCAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947071.1
    CDS168..4931
    Translation

    Target ORF information:

    RefSeq Version XM_018091582.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGAATATTT GTGGGGACTG TGCCAGTGGG CAGAACACTC ACCCACCCCC CATCGCAATG
    AAAGGACAGA AGGGAGAGCA AGGCCTACCT GGGATTCCTG GAACAGAGGA CTGTGAGAGG
    TGCCGACCTG AAAAAGAGGA TGAAGTGGGA GATGAGCCTG ATCATGGCTG TGCAGGTGGG
    CCAGGGCAAC CTGGGGCCCC AGGATTACCA GGACATGATG GACTACCTGG TCCACGAGGA
    CCCCCTGGAC TTCCAGGAGC TTCGGGTATG CAGGGAGAAA GAGGACCACC AGGGCCACAT
    GGAGAAAAAG GGGAATTGGG ACCACCTGGA CTTCCAGGTT ATCCTGGAAC TATGGGATCT
    CCAGGCCTTC CAGGTAGCAA AGGAGAGCGT GGCTACCATG GACCTGCTGG AGAGAAAGGA
    GAGTCGGGTC CTCCTGGTAT TCCGGGTGTT CCTGGTCCTG CTGGCCCTGC TGGACCAAGA
    GGTGAACGAG GAACTTCAGG GAGCTTCGGA GAAAAAGGTG ACCAGGGATT TTCAGGCCCC
    CCAGGTTTCC CAGGACCTGC GGGACCTCCA GGTTTTCCTG GAAAGACAGG GTCTCCTGGA
    CCACCTGGGC CTACTGCTGA AAAGGGTTCA GAAGGATTAC GTGGTTCTCC GGGACAACCT
    GGCTCATCAG GCCCACCAGG ACCTCCTGGA TTACAGGGGC CGCCTGGGAT TGAAGGACTG
    GACGGGAAGG ATGGGAAACC AGGACTGAGG GGAGAAATGG GACCTCCTGG GCCTCCTGGG
    CATAAGGGTA TTCCGGGATT CAAAGGGAAA ACAGGGCATC CTGGCTTGCC TGGGCAAAAG
    GGAGACTGTG GTCCTCCAGG TCCTTCAGGC ATTACTGGCA GGCCTGGTGC AGAGGGTGAT
    CCTGGTCCAA TGGGTCCACC AGGCCGCCCA GGTCCTTCTG GAAACTTGGG AGCTGCAGGA
    CCTACAGGAC CACCAGGTAA TCCAGGCCCC CCTGGAATGG GACTTAAAGG TGATCGAGGA
    CCACCAGGTG ATCGAGGACA ATTGGGTCTT CCGGGTGAGC CAGGTACCCC GGGACACCCA
    GGACCTGCTG GTGAAATGGG TTCAGATGGT CCTATGGGTA AGGAGGGTCC TTCTGGAAGG
    CCAGGAGCCC CTGGTTCTCC TGGAGAGAAG GGTGAAACCG GAATGCCTGG ATCTTCAGGC
    CCCCCAGGAG AAAGAGGAAG ACCAGGACCG CCAGGAACAT CAGCAGCTTA TACAAAAAGT
    GAATCATTAG GGATAGTTGG ACCAAGGGGA CCTCCAGGAG AGAGGGGCTC TTCTGGATCT
    CCAGGACCTG CTGGGGCACC AGGAAGCCCA GGACTTCCAG GAACACCTGC GGAATCCATC
    AGTTATGATG AGATTAAGAG AATGATACGA CAAGAGTTGA TCAAGATCTT TGATGAGAGG
    ATGGCATATT ATACAACCCG CATGCAGATG CCATTGGAGA TGGCTTCATC ACCAGGAAGA
    CCGGGCTCTC CTGGAAAGGA TGGATCCCCA GGACGTCCAG GACCCCCTGG ATCACCTGGA
    CTGCCAGGAC AAATGGGCCG TGAGGGGCGA CAAGGTGCCC CGGGAATGAG AGGAGAACCT
    GGATCAAAAG GAGAAAAGGG GGCAAATGGT ATTGGTGTCA TGGGAGATGT TGGACCTCCT
    GGCTCACCAG GACCACAAGG GCCACCTGGA TATGGCAAAC TGGGATTACC AGGACCAATG
    GGACAGCAAG GTATACCAGG TATTCCAGGT TCTCCAGGGG CAAAAGGTTA TCCAGGAAAA
    GATGGTAGAT GTAATCCCTC AGACTGCCAC GGTATGATGG GCGTGCAAGA GTACCACCCA
    AAGAGCATGA AGGGTCCAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42759
    Clone ID Related Accession (Same CDS sequence) XM_018091583.1
    Accession Version XM_018091583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGGCCCTCC TGGGCCACCA
    GGGCCTCCAG GTCCCCCAGG ACTTCCTGGT CTACAGGGAC CCCTGGCTGC AGAAAGCAAC
    ATTGATATCC TGAAGAATAT TTGTGGGGAC TGTGCCAGTG GGCAGAACAC TCACCCACCC
    CCCATCGCAA TGAAAGGACA GAAGGGAGAG CAAGGCCTAC CTGGGATTCC TGGAACAGAG
    GACTGTGAGA GGTGCCGACC TGAAAAAGAG GATGAAGTGG GAGATGAGCC TGATCATGGC
    TGTGCAGGTG GGCCAGGGCA ACCTGGGGCC CCAGGATTAC CAGGACATGA TGGACTACCT
    GGTCCACGAG GACCCCCTGG ACTTCCAGGA GCTTCGGGGC CCCCAGGTTT TCCAGGCACT
    CTTGGCACCC CCGGACTACC TGGTATGCAG GGAGAAAGAG GACCACCAGG GCCACATGGA
    GAAAAAGGGG AATTGGGACC ACCTGGACTT CCAGGTTATC CTGGAACTAT GGGATCTCCA
    GGCCTTCCAG GTAGCAAAGG AGAGCGTGGC TACCATGGAC CTGCTGGAGA GAAAGGAGAG
    TCGGGTCCTC CTGGTATTCC GGGTGTTCCT GGTCCTGCTG GCCCTGCTGG ACCAAGAGGT
    GAACGAGGAA CTTCAGGGAG CTTCGGAGAA AAAGGTGACC AGGGATTTTC AGGCCCCCCA
    GGTTTCCCAG GACCTGCGGG ACCTCCAGGT TTTCCTGGAA AGACAGGGTC TCCTGGACCA
    CCTGGGCCTA CTGCTGAAAA GGGTTCAGAA GGATTACGTG GTTCTCCGGG ACAACCTGGC
    TCATCAGGCC CACCAGGACC TCCTGGATTA CAGGGGCCGC CTGGGATTGA AGGACTGGAC
    GGGAAGGATG GGAAACCAGG ACTGAGGGGA GAAATGGGAC CTCCTGGGCC TCCTGGGCAT
    AAGGGTATTC CGGGATTCAA AGGGAAAACA GGGCATCCTG GCTTGCCTGG GCAAAAGGGA
    GACTGTGGTC CTCCAGGTCC TTCAGGCATT ACTGGCAGGC CTGGTGCAGA GGGTGATCCT
    GGTCCAATGG GTCCACCAGG CCGCCCAGGT CCTTCTGGAA ACTTGGGAGC TGCAGGACCT
    ACAGGACCAC CAGGTAATCC AGGCCCCCCT GGAATGGGAC TTAAAGGTGA TCGAGGACCA
    CCAGGTGATC GAGGACAATT GGGTCTTCCG GGTGAGCCAG GTACCCCGGG ACACCCAGGA
    CCTGCTGGTG AAATGGGTTC AGATGGTCCT ATGGGTAAGG AGGGTCCTTC TGGAAGGCCA
    GGAGCCCCTG GTTCTCCTGG AGAGAAGGGT GAAACCGGAA TGCCTGGATC TTCAGGCCCC
    CCAGGAGAAA GAGGAAGACC AGGACCGCCA GGAACATCAG CAGCTTATAC AAAAAGTGAA
    TCATTAGGGA TAGTTGGACC AAGGGGACCT CCAGGAGAGA GGGGCTCTTC TGGATCTCCA
    GGACCTGCTG GGGCACCAGG AAGCCCAGGA CTTCCAGGAA CACCTGCGGA ATCCATCAGT
    TATGATGAGA TTAAGAGAAT GATACGACAA GAGTTGATCA AGATCTTTGA TGAGAGGATG
    GCATATTATA CAACCCGCAT GCAGATGCCA TTGGAGATGG CTTCATCACC AGGAAGACCG
    GGCTCTCCTG GAAAGGATGG ATCCCCAGGA CGTCCAGGAC CCCCTGGATC ACCTGGACTG
    CCAGGACAAA TGGGCCGTGA GGGGCGACAA GGTGCCCCGG GAATGAGAGG AGAACCTGGA
    TCAAAAGGAG AAAAGGGGGC AAATGGTATT GGTGTCATGG GAGATGTTGG ACCTCCTGGC
    TCACCAGGAC CACAAGGGCC ACCTGGATAT GGCAAACTGG GATTACCAGG ACCAATGGGA
    CAGCAAGGTA TACCAGGTAT TCCAGGTTCT CCAGGGGCAA AAGGTTATCC AGGAAAAGAT
    GGTAGATGTA ATCCCTCAGA CTGCCACGGT ATGATGGGCG TGCAAGAGTA CCACCCAAAG
    AGCATGAAGG GTCCAGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947072.1
    CDS168..4928
    Translation

    Target ORF information:

    RefSeq Version XM_018091583.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGGCCCTCC TGGGCCACCA
    GGGCCTCCAG GTCCCCCAGG ACTTCCTGGT CTACAGGGAC CCCTGGCTGC AGAAAGCAAC
    ATTGATATCC TGAAGAATAT TTGTGGGGAC TGTGCCAGTG GGCAGAACAC TCACCCACCC
    CCCATCGCAA TGAAAGGACA GAAGGGAGAG CAAGGCCTAC CTGGGATTCC TGGAACAGAG
    GACTGTGAGA GGTGCCGACC TGAAAAAGAG GATGAAGTGG GAGATGAGCC TGATCATGGC
    TGTGCAGGTG GGCCAGGGCA ACCTGGGGCC CCAGGATTAC CAGGACATGA TGGACTACCT
    GGTCCACGAG GACCCCCTGG ACTTCCAGGA GCTTCGGGGC CCCCAGGTTT TCCAGGCACT
    CTTGGCACCC CCGGACTACC TGGTATGCAG GGAGAAAGAG GACCACCAGG GCCACATGGA
    GAAAAAGGGG AATTGGGACC ACCTGGACTT CCAGGTTATC CTGGAACTAT GGGATCTCCA
    GGCCTTCCAG GTAGCAAAGG AGAGCGTGGC TACCATGGAC CTGCTGGAGA GAAAGGAGAG
    TCGGGTCCTC CTGGTATTCC GGGTGTTCCT GGTCCTGCTG GCCCTGCTGG ACCAAGAGGT
    GAACGAGGAA CTTCAGGGAG CTTCGGAGAA AAAGGTGACC AGGGATTTTC AGGCCCCCCA
    GGTTTCCCAG GACCTGCGGG ACCTCCAGGT TTTCCTGGAA AGACAGGGTC TCCTGGACCA
    CCTGGGCCTA CTGCTGAAAA GGGTTCAGAA GGATTACGTG GTTCTCCGGG ACAACCTGGC
    TCATCAGGCC CACCAGGACC TCCTGGATTA CAGGGGCCGC CTGGGATTGA AGGACTGGAC
    GGGAAGGATG GGAAACCAGG ACTGAGGGGA GAAATGGGAC CTCCTGGGCC TCCTGGGCAT
    AAGGGTATTC CGGGATTCAA AGGGAAAACA GGGCATCCTG GCTTGCCTGG GCAAAAGGGA
    GACTGTGGTC CTCCAGGTCC TTCAGGCATT ACTGGCAGGC CTGGTGCAGA GGGTGATCCT
    GGTCCAATGG GTCCACCAGG CCGCCCAGGT CCTTCTGGAA ACTTGGGAGC TGCAGGACCT
    ACAGGACCAC CAGGTAATCC AGGCCCCCCT GGAATGGGAC TTAAAGGTGA TCGAGGACCA
    CCAGGTGATC GAGGACAATT GGGTCTTCCG GGTGAGCCAG GTACCCCGGG ACACCCAGGA
    CCTGCTGGTG AAATGGGTTC AGATGGTCCT ATGGGTAAGG AGGGTCCTTC TGGAAGGCCA
    GGAGCCCCTG GTTCTCCTGG AGAGAAGGGT GAAACCGGAA TGCCTGGATC TTCAGGCCCC
    CCAGGAGAAA GAGGAAGACC AGGACCGCCA GGAACATCAG CAGCTTATAC AAAAAGTGAA
    TCATTAGGGA TAGTTGGACC AAGGGGACCT CCAGGAGAGA GGGGCTCTTC TGGATCTCCA
    GGACCTGCTG GGGCACCAGG AAGCCCAGGA CTTCCAGGAA CACCTGCGGA ATCCATCAGT
    TATGATGAGA TTAAGAGAAT GATACGACAA GAGTTGATCA AGATCTTTGA TGAGAGGATG
    GCATATTATA CAACCCGCAT GCAGATGCCA TTGGAGATGG CTTCATCACC AGGAAGACCG
    GGCTCTCCTG GAAAGGATGG ATCCCCAGGA CGTCCAGGAC CCCCTGGATC ACCTGGACTG
    CCAGGACAAA TGGGCCGTGA GGGGCGACAA GGTGCCCCGG GAATGAGAGG AGAACCTGGA
    TCAAAAGGAG AAAAGGGGGC AAATGGTATT GGTGTCATGG GAGATGTTGG ACCTCCTGGC
    TCACCAGGAC CACAAGGGCC ACCTGGATAT GGCAAACTGG GATTACCAGG ACCAATGGGA
    CAGCAAGGTA TACCAGGTAT TCCAGGTTCT CCAGGGGCAA AAGGTTATCC AGGAAAAGAT
    GGTAGATGTA ATCCCTCAGA CTGCCACGGT ATGATGGGCG TGCAAGAGTA CCACCCAAAG
    AGCATGAAGG GTCCAGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42760
    Clone ID Related Accession (Same CDS sequence) XM_018091584.1
    Accession Version XM_018091584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG ATTCAATCTT GTCCGTAGAT TTTCTCTCCA GAAGATGAGC
    TCAGTAAAGA AGATTCGTAA CCCTAAAGGA CCTATTATCA TGCGTCTTGG AGGGGCCAAG
    TTAGTGGAGC CAACATCACA GGTGTTCCCT CACGGTGTCC CTGATGAGTT TACACTTGTC
    ATATCCTTGT TGTTAAAGAA GCAGACAACT GATGAAGATT GGTACCTCTT TCAAATCAGT
    GACCAGATGG GATACCCACA GGTCTCACTC AGTATTAATG GAGTTGACAG GAGTCTGGAA
    TTTCGTTCCA AAGGACGTGA CAAAGAGTAT GTGAGCTGTA CATTTGCTGG GCATGGGGTG
    TATTCTTTAT TTGACAACGG ATGGCACAAG ATTGTACTAA GTGTGCAGGA GAAAATGGTG
    TCTGTTCACA TCGACTGCAG CTTCATCTCC AGCAAACCCA TTGAGCCCAG AGGGGCACTG
    GCCAGTGAAG GACACACCTT TATAGGTTTG GATGCAGCTA ATGGTGTACC AGTTCATTTT
    GATATTCAGA AGTTCCTTCT GTACTGTGAT CCCCTAATGG CAATGCAAGA AGGGTGCTGT
    GAGATCCTAC CATCTGGTTG CAACCCTGAG GCTCCCAAGA CAAGAAGAAA TGTGGAAGTG
    GCTGAAAGTG TACAGAATGC CAACCTTATT GACATACCTC ATAAAACCAA GCAGCAAGGA
    TACACTCGAT GCTTCTGTCT GGAGGAGGCT TTGGAAAGAA GGGGTGGAAA AGTGTCTTCT
    GGAATGCCTA TTGAGTTGCC TGCAGAGTGT GCATCATGCG CCTTCCAGCT CCCTGAGAGA
    AATGTCACAG TGGGACCTCC TGGCCCCAGG GGTTTGAAAG GGGAAAGAGG GCAGTCTGGA
    ACTTATGGAC CAAAAGGAGA GAAAGGAGAG AGGGGGGCAG ACTGTGTTCG GACATCAGCT
    CAAGGACCAT TACAGTGTGC TGAGGGTACA AAGGGAGAAA AGGGAGAACG TGGGCAAGAG
    GGTCCTAGAG GATACGAAGG ACCATCAGGA CAAGATGGAG AGAAGGGACA AAAAGGTGAA
    AGGGGAGATT TAGGATTGAA AGGATCAGTT GGATTACCAG GACGAGATGG AAGGCCTGGA
    GAAATCTGTG TGATGGGACC TAAAGGGCAA AAGGGAGCGA CTGGCATTGT TGGTCCAGAA
    GGTCTTGCAG GAGAACCAGG TCCTCCAGGA AAACCAGGAC CTCCAGGAAC TGGGCTGCCA
    GGAAAACCTG GTGATCCTGG TGGCCCACCT GGCCCTCCTG GAGAAAAGGG CACTATAGGA
    ACACCTGGAA GAGATGGGGC TGTTGGAAAA CCGGGCAAGC CAGGTATCCC AGGACACAAA
    GGAGAGAAGG GGGATCAATG TGAGCCGTGT GCATCTGTGT CTACTGATGG AACAAACGTG
    ATCAGTATGC CAGGCCAAAA AGGGCAGAAA GGAGAAATGG GAATCCCAGG AACTGGGGAG
    CCTGGGAAAG CAGGAATCAA AGGTGAATCA GGCATAAAAG GTGAACAGGG TTCCTCCGGT
    ATTAATGGTA TAAAAGGGGA GAAGGGGGAG TCGTGCACTT CTTGTCCGAC ATTGTCTCCA
    GGCATGGCCT CCATACAGAA CATACCAAAG GGCCTGAAGG GAGAACCTGG GGAGCCTGGA
    GTTGGACTGA CTGGACTACC GGGAAAAACT GGGGTACCTG GATCATCTGG ACCCAAAGGA
    GAAAAGGGGG ATTTTGGGCC TCACGGACCA CCTGGAACAC ATGGCTTGCC AGGAGATCAA
    GGGAGAATTG GACCAGCTGG AACTAAAGGA GAAAAGGGTG AGCCCTGCAC TATGTGCTCA
    TCTCTCCCAG CTGAAGTAAC AAAGCCAGAT ACTGTCATTG CTATTCCTGG ACCCCCAGGA
    CAAAAGGGAG AACCAGGAGA ATCAGGAAAT GGAATACCAG GAAAAAATGG CAAACAAGGG
    GAACTTGGAA TGAAGGGGCA GAAAGGTGCG ACTGGAGCAG CAGGGGACCC TGGCATTCCT
    GGGAGCACAG GTTTACCAGG GTTGGCAGGA CAGCCAGGAA TACAAGGATT TGAGGGACCT
    AAGGGGCAGA AGGGAGATGC CTGTGACTCA TGTCCCATGA TTGGTGGAGG AGATTCTAAT
    GGATCGGGTA TTCCTGGGAA AAAAGGGGAA CAAGGGCCTC CGGGGATAGG ACAGCCAGGA
    AAACCAGGTC AGTCAGGACT TCCTGGAATT CAGGGACCAC AAGGAATTAA AGGTGCCCAG
    GGGGAGCCAG GACCACCAGG AGAAGGGAAA AGAGGTCCAC AGGGAGAATC TGGAGAGCGT
    GGTTTGCCTG GGAAAACAGG GCCACCAGGA CCTCAAGGGT CGCCTGGCTT TGTTGGATCC
    AAAGGGGAAA AGGGAAGCAC TGGACAAAAG GGGGCCTCAG GAGCACCTGG AAATTCTGGA
    ACAGGTGTCA TGGGACCACC GGGACGGGAT GGCACTTCAG GCCAACCAGG AATTCCTGGA
    CGTGATGGTG TACCGGGTGA GAGAGGAGAA AAGGGAGATG CTGGAGTGTG TTCCTGCCCA
    CCAGGGCCAT GGATGGATCC AGAAATTTTT GGAAGACCGG ACTCTCCTAA TAAGTGGAAT
    GGAGATGTCT GGCCTTTTGG AATTCAGGGC CCTCCTGGGC CACCAGGGCC TCCAGGTCCC
    CCAGGACTTC CTGGTCTACA GGGACCCCTG GCTGCAGAAA GCAACATTGA TATCCTGAAG
    AATATTTGTG GGGACTGTGC CAGTGGGCAG AACACTCACC CACCCCCCAT CGCAATGAAA
    GGACAGAAGG GAGAGCAAGG CCTACCTGGG ATTCCTGGAA CAGAGGACTG TGAGAGGTGC
    CGACCTGAAA AAGAGGATGA AGTGGGAGAT GAGCCTGATC ATGGCTGTGC AGGTGGGCCA
    GGGCAACCTG GGGCCCCAGG ATTACCAGGA CATGATGGAC TACCTGGTCC ACGAGGACCC
    CCTGGACTTC CAGGAGCTTC GGGGCCCCCA GGTTTTCCAG GCACTCTTGG CACCCCCGGA
    CTACCTGGTA TGCAGGGAGA AAGAGGACCA CCAGGGCCAC ATGGAGAAAA AGGGGAATTG
    GGACCACCTG GACTTCCAGG TTATCCTGGA ACTATGGGAT CTCCAGGCCT TCCAGGTAGC
    AAAGGAGAGC GTGGCTACCA TGGACCTGCT GGAGAGAAAG GAGAGTCGGG TCCTCCTGGT
    ATTCCGGGTG TTCCTGGTCC TGCTGGCCCT GCTGGACCAA GAGGTGAACG AGGAACTTCA
    GGGAGCTTCG GAGAAAAAGG TGACCAGGGA TTTTCAGGCC CCCCAGGTTT CCCAGGACCT
    GCGGGACCTC CAGGTTTTCC TGGAAAGACA GGGTCTCCTG GACCACCTGG GCCTACTGCT
    GAAAAGGGTT CAGAAGGATT ACGTGGTTCT CCGGGACAAC CTGGCTCATC AGGCCCACCA
    GGACCTCCTG GATTACAGGG GCCGCCTGGG ATTGAAGGAC TGGACGGGAA GGATGGGAAA
    CCAGGACTGA GGGGAGAAAT GGGACCTCCT GGGCCTCCTG GGCATAAGGG TATTCCGGGA
    TTCAAAGGGA AAACAGGGCA TCCTGGCTTG CCTGGGCAAA AGGGAGACTG TGGTCCTCCA
    GGTCCTTCAG GCATTACTGG CAGGCCTGGT GCAGAGGGTG ATCCTGGTCC AATGGGTCCA
    CCAGGCCGCC CAGGTCCTTC TGGAAACTTG GGAGCTGCAG GACCTACAGG ACCACCAGGT
    AATCCAGGCC CCCCTGGAAT GGGACTTAAA GGTGATCGAG GACCACCAGG TGATCGAGGA
    CAATTGGGTC TTCCGGGTGA GCCAGGTACC CCGGGACACC CAGGACCTGC TGGTGAAATG
    GGTTCAGATG GTCCTATGGG TAAGGAGGGT CCTTCTGGAA GGCCAGGAGC CCCTGGTTCT
    CCTGGAGAGA AGGGTGAAAC CGGAATGCCT GGATCTTCAG GCCCCCCAGG AGAAAGAGGA
    AGACCAGGAC CGCCAGGAAC ATCAGCAGCT TATACAAAAA GTGAATCATT AGGGATAGTT
    GGACCAAGGG GACCTCCAGG AGAGAGGGGC TCTTCTGGAT CTCCAGGACC TGCTGGGGCA
    CCAGGAAGCC CAGGACTTCC AGGAACACCT GCGGAATCCA TCAGTTATGA TGAGATTAAG
    AGAATGATAC GACAAGAGTT GATCAAGATC TTTGATGAGA GGATGGCATA TTATACAACC
    CGCATGCAGA TGCCATTGGA GATGGCTTCA TCACCAGGAA GACCGGGCTC TCCTGGAAAG
    GATGGATCCC CAGGACGTCC AGGACCCCCT GGATCACCTG GACTGCCAGG ACAAATGGGC
    CGTGAGGGGC GACAAGGTGC CCCGGGAATG AGAGGAGAAC CTGGATCAAA AGGAGAAAAG
    GGGGCAAATG GTATTGGTGT CATGGGAGAT GTTGGACCTC CTGGCTCACC AGGACCACAA
    GGGCCACCTG GATATGGCAA ACTGGGATTA CCAGGACCAA TGGGACAGCA AGGTATACCA
    GGTATTCCAG GTTCTCCAGG GGCAAAAGGT TATCCAGGAA AAGATGGTAG ATGTAATCCC
    TCAGACTGCC ACGGTATGAT GGGCGTGCAA GAGTACCACC CAAAGAGCAT GAAGGGTCCA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947073.1
    CDS168..4913
    Translation

    Target ORF information:

    RefSeq Version XM_018091584.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG ATTCAATCTT GTCCGTAGAT TTTCTCTCCA GAAGATGAGC
    TCAGTAAAGA AGATTCGTAA CCCTAAAGGA CCTATTATCA TGCGTCTTGG AGGGGCCAAG
    TTAGTGGAGC CAACATCACA GGTGTTCCCT CACGGTGTCC CTGATGAGTT TACACTTGTC
    ATATCCTTGT TGTTAAAGAA GCAGACAACT GATGAAGATT GGTACCTCTT TCAAATCAGT
    GACCAGATGG GATACCCACA GGTCTCACTC AGTATTAATG GAGTTGACAG GAGTCTGGAA
    TTTCGTTCCA AAGGACGTGA CAAAGAGTAT GTGAGCTGTA CATTTGCTGG GCATGGGGTG
    TATTCTTTAT TTGACAACGG ATGGCACAAG ATTGTACTAA GTGTGCAGGA GAAAATGGTG
    TCTGTTCACA TCGACTGCAG CTTCATCTCC AGCAAACCCA TTGAGCCCAG AGGGGCACTG
    GCCAGTGAAG GACACACCTT TATAGGTTTG GATGCAGCTA ATGGTGTACC AGTTCATTTT
    GATATTCAGA AGTTCCTTCT GTACTGTGAT CCCCTAATGG CAATGCAAGA AGGGTGCTGT
    GAGATCCTAC CATCTGGTTG CAACCCTGAG GCTCCCAAGA CAAGAAGAAA TGTGGAAGTG
    GCTGAAAGTG TACAGAATGC CAACCTTATT GACATACCTC ATAAAACCAA GCAGCAAGGA
    TACACTCGAT GCTTCTGTCT GGAGGAGGCT TTGGAAAGAA GGGGTGGAAA AGTGTCTTCT
    GGAATGCCTA TTGAGTTGCC TGCAGAGTGT GCATCATGCG CCTTCCAGCT CCCTGAGAGA
    AATGTCACAG TGGGACCTCC TGGCCCCAGG GGTTTGAAAG GGGAAAGAGG GCAGTCTGGA
    ACTTATGGAC CAAAAGGAGA GAAAGGAGAG AGGGGGGCAG ACTGTGTTCG GACATCAGCT
    CAAGGACCAT TACAGTGTGC TGAGGGTACA AAGGGAGAAA AGGGAGAACG TGGGCAAGAG
    GGTCCTAGAG GATACGAAGG ACCATCAGGA CAAGATGGAG AGAAGGGACA AAAAGGTGAA
    AGGGGAGATT TAGGATTGAA AGGATCAGTT GGATTACCAG GACGAGATGG AAGGCCTGGA
    GAAATCTGTG TGATGGGACC TAAAGGGCAA AAGGGAGCGA CTGGCATTGT TGGTCCAGAA
    GGTCTTGCAG GAGAACCAGG TCCTCCAGGA AAACCAGGAC CTCCAGGAAC TGGGCTGCCA
    GGAAAACCTG GTGATCCTGG TGGCCCACCT GGCCCTCCTG GAGAAAAGGG CACTATAGGA
    ACACCTGGAA GAGATGGGGC TGTTGGAAAA CCGGGCAAGC CAGGTATCCC AGGACACAAA
    GGAGAGAAGG GGGATCAATG TGAGCCGTGT GCATCTGTGT CTACTGATGG AACAAACGTG
    ATCAGTATGC CAGGCCAAAA AGGGCAGAAA GGAGAAATGG GAATCCCAGG AACTGGGGAG
    CCTGGGAAAG CAGGAATCAA AGGTGAATCA GGCATAAAAG GTGAACAGGG TTCCTCCGGT
    ATTAATGGTA TAAAAGGGGA GAAGGGGGAG TCGTGCACTT CTTGTCCGAC ATTGTCTCCA
    GGCATGGCCT CCATACAGAA CATACCAAAG GGCCTGAAGG GAGAACCTGG GGAGCCTGGA
    GTTGGACTGA CTGGACTACC GGGAAAAACT GGGGTACCTG GATCATCTGG ACCCAAAGGA
    GAAAAGGGGG ATTTTGGGCC TCACGGACCA CCTGGAACAC ATGGCTTGCC AGGAGATCAA
    GGGAGAATTG GACCAGCTGG AACTAAAGGA GAAAAGGGTG AGCCCTGCAC TATGTGCTCA
    TCTCTCCCAG CTGAAGTAAC AAAGCCAGAT ACTGTCATTG CTATTCCTGG ACCCCCAGGA
    CAAAAGGGAG AACCAGGAGA ATCAGGAAAT GGAATACCAG GAAAAAATGG CAAACAAGGG
    GAACTTGGAA TGAAGGGGCA GAAAGGTGCG ACTGGAGCAG CAGGGGACCC TGGCATTCCT
    GGGAGCACAG GTTTACCAGG GTTGGCAGGA CAGCCAGGAA TACAAGGATT TGAGGGACCT
    AAGGGGCAGA AGGGAGATGC CTGTGACTCA TGTCCCATGA TTGGTGGAGG AGATTCTAAT
    GGATCGGGTA TTCCTGGGAA AAAAGGGGAA CAAGGGCCTC CGGGGATAGG ACAGCCAGGA
    AAACCAGGTC AGTCAGGACT TCCTGGAATT CAGGGACCAC AAGGAATTAA AGGTGCCCAG
    GGGGAGCCAG GACCACCAGG AGAAGGGAAA AGAGGTCCAC AGGGAGAATC TGGAGAGCGT
    GGTTTGCCTG GGAAAACAGG GCCACCAGGA CCTCAAGGGT CGCCTGGCTT TGTTGGATCC
    AAAGGGGAAA AGGGAAGCAC TGGACAAAAG GGGGCCTCAG GAGCACCTGG AAATTCTGGA
    ACAGGTGTCA TGGGACCACC GGGACGGGAT GGCACTTCAG GCCAACCAGG AATTCCTGGA
    CGTGATGGTG TACCGGGTGA GAGAGGAGAA AAGGGAGATG CTGGAGTGTG TTCCTGCCCA
    CCAGGGCCAT GGATGGATCC AGAAATTTTT GGAAGACCGG ACTCTCCTAA TAAGTGGAAT
    GGAGATGTCT GGCCTTTTGG AATTCAGGGC CCTCCTGGGC CACCAGGGCC TCCAGGTCCC
    CCAGGACTTC CTGGTCTACA GGGACCCCTG GCTGCAGAAA GCAACATTGA TATCCTGAAG
    AATATTTGTG GGGACTGTGC CAGTGGGCAG AACACTCACC CACCCCCCAT CGCAATGAAA
    GGACAGAAGG GAGAGCAAGG CCTACCTGGG ATTCCTGGAA CAGAGGACTG TGAGAGGTGC
    CGACCTGAAA AAGAGGATGA AGTGGGAGAT GAGCCTGATC ATGGCTGTGC AGGTGGGCCA
    GGGCAACCTG GGGCCCCAGG ATTACCAGGA CATGATGGAC TACCTGGTCC ACGAGGACCC
    CCTGGACTTC CAGGAGCTTC GGGGCCCCCA GGTTTTCCAG GCACTCTTGG CACCCCCGGA
    CTACCTGGTA TGCAGGGAGA AAGAGGACCA CCAGGGCCAC ATGGAGAAAA AGGGGAATTG
    GGACCACCTG GACTTCCAGG TTATCCTGGA ACTATGGGAT CTCCAGGCCT TCCAGGTAGC
    AAAGGAGAGC GTGGCTACCA TGGACCTGCT GGAGAGAAAG GAGAGTCGGG TCCTCCTGGT
    ATTCCGGGTG TTCCTGGTCC TGCTGGCCCT GCTGGACCAA GAGGTGAACG AGGAACTTCA
    GGGAGCTTCG GAGAAAAAGG TGACCAGGGA TTTTCAGGCC CCCCAGGTTT CCCAGGACCT
    GCGGGACCTC CAGGTTTTCC TGGAAAGACA GGGTCTCCTG GACCACCTGG GCCTACTGCT
    GAAAAGGGTT CAGAAGGATT ACGTGGTTCT CCGGGACAAC CTGGCTCATC AGGCCCACCA
    GGACCTCCTG GATTACAGGG GCCGCCTGGG ATTGAAGGAC TGGACGGGAA GGATGGGAAA
    CCAGGACTGA GGGGAGAAAT GGGACCTCCT GGGCCTCCTG GGCATAAGGG TATTCCGGGA
    TTCAAAGGGA AAACAGGGCA TCCTGGCTTG CCTGGGCAAA AGGGAGACTG TGGTCCTCCA
    GGTCCTTCAG GCATTACTGG CAGGCCTGGT GCAGAGGGTG ATCCTGGTCC AATGGGTCCA
    CCAGGCCGCC CAGGTCCTTC TGGAAACTTG GGAGCTGCAG GACCTACAGG ACCACCAGGT
    AATCCAGGCC CCCCTGGAAT GGGACTTAAA GGTGATCGAG GACCACCAGG TGATCGAGGA
    CAATTGGGTC TTCCGGGTGA GCCAGGTACC CCGGGACACC CAGGACCTGC TGGTGAAATG
    GGTTCAGATG GTCCTATGGG TAAGGAGGGT CCTTCTGGAA GGCCAGGAGC CCCTGGTTCT
    CCTGGAGAGA AGGGTGAAAC CGGAATGCCT GGATCTTCAG GCCCCCCAGG AGAAAGAGGA
    AGACCAGGAC CGCCAGGAAC ATCAGCAGCT TATACAAAAA GTGAATCATT AGGGATAGTT
    GGACCAAGGG GACCTCCAGG AGAGAGGGGC TCTTCTGGAT CTCCAGGACC TGCTGGGGCA
    CCAGGAAGCC CAGGACTTCC AGGAACACCT GCGGAATCCA TCAGTTATGA TGAGATTAAG
    AGAATGATAC GACAAGAGTT GATCAAGATC TTTGATGAGA GGATGGCATA TTATACAACC
    CGCATGCAGA TGCCATTGGA GATGGCTTCA TCACCAGGAA GACCGGGCTC TCCTGGAAAG
    GATGGATCCC CAGGACGTCC AGGACCCCCT GGATCACCTG GACTGCCAGG ACAAATGGGC
    CGTGAGGGGC GACAAGGTGC CCCGGGAATG AGAGGAGAAC CTGGATCAAA AGGAGAAAAG
    GGGGCAAATG GTATTGGTGT CATGGGAGAT GTTGGACCTC CTGGCTCACC AGGACCACAA
    GGGCCACCTG GATATGGCAA ACTGGGATTA CCAGGACCAA TGGGACAGCA AGGTATACCA
    GGTATTCCAG GTTCTCCAGG GGCAAAAGGT TATCCAGGAA AAGATGGTAG ATGTAATCCC
    TCAGACTGCC ACGGTATGAT GGGCGTGCAA GAGTACCACC CAAAGAGCAT GAAGGGTCCA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42761
    Clone ID Related Accession (Same CDS sequence) XM_018091585.1
    Accession Version XM_018091585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XVI) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGTGCCGAC CTGAAAAAGA GGATGAAGTG GGAGATGAGC CTGATCATGG CTGTGCAGGT
    GGGCCAGGGC AACCTGGGGC CCCAGGATTA CCAGGACATG ATGGACTACC TGGTCCACGA
    GGACCCCCTG GACTTCCAGG AGCTTCGGGG CCCCCAGGTT TTCCAGGCAC TCTTGGCACC
    CCCGGACTAC CTGGTATGCA GGGAGAAAGA GGACCACCAG GGCCACATGG AGAAAAAGGG
    GAATTGGGAC CACCTGGACT TCCAGGTTAT CCTGGAACTA TGGGATCTCC AGGCCTTCCA
    GGTAGCAAAG GAGAGCGTGG CTACCATGGA CCTGCTGGAG AGAAAGGAGA GTCGGGTCCT
    CCTGGTATTC CGGGTGTTCC TGGTCCTGCT GGCCCTGCTG GACCAAGAGG TGAACGAGGA
    ACTTCAGGGA GCTTCGGAGA AAAAGGTGAC CAGGGATTTT CAGGCCCCCC AGGTTTCCCA
    GGACCTGCGG GACCTCCAGG TTTTCCTGGA AAGACAGGGT CTCCTGGACC ACCTGGGCCT
    ACTGCTGAAA AGGGTTCAGA AGGATTACGT GGTTCTCCGG GACAACCTGG CTCATCAGGC
    CCACCAGGAC CTCCTGGATT ACAGGGGCCG CCTGGGATTG AAGGACTGGA CGGGAAGGAT
    GGGAAACCAG GACTGAGGGG AGAAATGGGA CCTCCTGGGC CTCCTGGGCA TAAGGGTATT
    CCGGGATTCA AAGGGAAAAC AGGGCATCCT GGCTTGCCTG GGCAAAAGGG AGACTGTGGT
    CCTCCAGGTC CTTCAGGCAT TACTGGCAGG CCTGGTGCAG AGGGTGATCC TGGTCCAATG
    GGTCCACCAG GCCGCCCAGG TCCTTCTGGA AACTTGGGAG CTGCAGGACC TACAGGACCA
    CCAGGTAATC CAGGCCCCCC TGGAATGGGA CTTAAAGGTG ATCGAGGACC ACCAGGTGAT
    CGAGGACAAT TGGGTCTTCC GGGTGAGCCA GGTACCCCGG GACACCCAGG ACCTGCTGGT
    GAAATGGGTT CAGATGGTCC TATGGGTAAG GAGGGTCCTT CTGGAAGGCC AGGAGCCCCT
    GGTTCTCCTG GAGAGAAGGG TGAAACCGGA ATGCCTGGAT CTTCAGGCCC CCCAGGAGAA
    AGAGGAAGAC CAGGACCGCC AGGAACATCA GCAGCTTATA CAAAAAGTGA ATCATTAGGG
    ATAGTTGGAC CAAGGGGACC TCCAGGAGAG AGGGGCTCTT CTGGATCTCC AGGACCTGCT
    GGGGCACCAG GAAGCCCAGG ACTTCCAGGA ACACCTGCGG AATCCATCAG TTATGATGAG
    ATTAAGAGAA TGATACGACA AGAGTTGATC AAGATCTTTG ATGAGAGGAT GGCATATTAT
    ACAACCCGCA TGCAGATGCC ATTGGAGATG GCTTCATCAC CAGGAAGACC GGGCTCTCCT
    GGAAAGGATG GATCCCCAGG ACGTCCAGGA CCCCCTGGAT CACCTGGACT GCCAGGACAA
    ATGGGCCGTG AGGGGCGACA AGGTGCCCCG GGAATGAGAG GAGAACCTGG ATCAAAAGGA
    GAAAAGGGGG CAAATGGTAT TGGTGTCATG GGAGATGTTG GACCTCCTGG CTCACCAGGA
    CCACAAGGGC CACCTGGATA TGGCAAACTG GGATTACCAG GACCAATGGG ACAGCAAGGT
    ATACCAGGTA TTCCAGGTTC TCCAGGGGCA AAAGGTTATC CAGGAAAAGA TGGTAGATGT
    AATCCCTCAG ACTGCCACGG TATGATGGGC GTGCAAGAGT ACCACCCAAA GAGCATGAAG
    GGTCCAGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947074.1
    CDS168..4859
    Translation

    Target ORF information:

    RefSeq Version XM_018091585.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XVI, alpha 1 (col16a1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTGGGTAC TCCTGACGTC TGTGTTCTGG TTGCTCAGGT GCTTAGCTTT CAGCGAAGGA 
    AGAATATTAC CTCACACAGG AGAACAGTGT CCAAGTTTAC AACACGAAGG AATAAACACC
    GATGGAAACT CAAATAGCAC AGGATTCAAT CTTGTCCGTA GATTTTCTCT CCAGAAGATG
    AGCTCAGTAA AGAAGATTCG TAACCCTAAA GGACCTATTA TCATGCGTCT TGGAGGGGCC
    AAGTTAGTGG AGCCAACATC ACAGGTGTTC CCTCACGGTG TCCCTGATGA GTTTACACTT
    GTCATATCCT TGTTGTTAAA GAAGCAGACA ACTGATGAAG ATTGGTACCT CTTTCAAATC
    AGTGACCAGA TGGGATACCC ACAGGTCTCA CTCAGTATTA ATGGAGTTGA CAGGAGTCTG
    GAATTTCGTT CCAAAGGACG TGACAAAGAG TATGTGAGCT GTACATTTGC TGGGCATGGG
    GTGTATTCTT TATTTGACAA CGGATGGCAC AAGATTGTAC TAAGTGTGCA GGAGAAAATG
    GTGTCTGTTC ACATCGACTG CAGCTTCATC TCCAGCAAAC CCATTGAGCC CAGAGGGGCA
    CTGGCCAGTG AAGGACACAC CTTTATAGGT TTGGATGCAG CTAATGGTGT ACCAGTTCAT
    TTTGATATTC AGAAGTTCCT TCTGTACTGT GATCCCCTAA TGGCAATGCA AGAAGGGTGC
    TGTGAGATCC TACCATCTGG TTGCAACCCT GAGGCTCCCA AGACAAGAAG AAATGTGGAA
    GTGGCTGAAA GTGTACAGAA TGCCAACCTT ATTGACATAC CTCATAAAAC CAAGCAGCAA
    GGATACACTC GATGCTTCTG TCTGGAGGAG GCTTTGGAAA GAAGGGGTGG AAAAGTGTCT
    TCTGGAATGC CTATTGAGTT GCCTGCAGAG TGTGCATCAT GCGCCTTCCA GCTCCCTGAG
    AGAAATGTCA CAGTGGGACC TCCTGGCCCC AGGGGTTTGA AAGGGGAAAG AGGGCAGTCT
    GGAACTTATG GACCAAAAGG AGAGAAAGGA GAGAGGGGGG CAGACTGTGT TCGGACATCA
    GCTCAAGGAC CATTACAGTG TGCTGAGGGT ACAAAGGGAG AAAAGGGAGA ACGTGGGCAA
    GAGGGTCCTA GAGGATACGA AGGACCATCA GGACAAGATG GAGAGAAGGG ACAAAAAGGT
    GAAAGGGGAG ATTTAGGATT GAAAGGATCA GTTGGATTAC CAGGACGAGA TGGAAGGCCT
    GGAGAAATCT GTGTGATGGG ACCTAAAGGG CAAAAGGGAG CGACTGGCAT TGTTGGTCCA
    GAAGGTCTTG CAGGAGAACC AGGTCCTCCA GGAAAACCAG GACCTCCAGG AACTGGGCTG
    CCAGGAAAAC CTGGTGATCC TGGTGGCCCA CCTGGCCCTC CTGGAGAAAA GGGCACTATA
    GGAACACCTG GAAGAGATGG GGCTGTTGGA AAACCGGGCA AGCCAGGTAT CCCAGGACAC
    AAAGGAGAGA AGGGGGATCA ATGTGAGCCG TGTGCATCTG TGTCTACTGA TGGAACAAAC
    GTGATCAGTA TGCCAGGCCA AAAAGGGCAG AAAGGAGAAA TGGGAATCCC AGGAACTGGG
    GAGCCTGGGA AAGCAGGAAT CAAAGGTGAA TCAGGCATAA AAGGTGAACA GGGTTCCTCC
    GGTATTAATG GTATAAAAGG GGAGAAGGGG GAGTCGTGCA CTTCTTGTCC GACATTGTCT
    CCAGGCATGG CCTCCATACA GAACATACCA AAGGGCCTGA AGGGAGAACC TGGGGAGCCT
    GGAGTTGGAC TGACTGGACT ACCGGGAAAA ACTGGGGTAC CTGGATCATC TGGACCCAAA
    GGAGAAAAGG GGGATTTTGG GCCTCACGGA CCACCTGGAA CACATGGCTT GCCAGGAGAT
    CAAGGGAGAA TTGGACCAGC TGGAACTAAA GGAGAAAAGG GTGAGCCCTG CACTATGTGC
    TCATCTCTCC CAGCTGAAGT AACAAAGCCA GATACTGTCA TTGCTATTCC TGGACCCCCA
    GGACAAAAGG GAGAACCAGG AGAATCAGGA AATGGAATAC CAGGAAAAAA TGGCAAACAA
    GGGGAACTTG GAATGAAGGG GCAGAAAGGT GCGACTGGAG CAGCAGGGGA CCCTGGCATT
    CCTGGGAGCA CAGGTTTACC AGGGTTGGCA GGACAGCCAG GAATACAAGG ATTTGAGGGA
    CCTAAGGGGC AGAAGGGAGA TGCCTGTGAC TCATGTCCCA TGATTGGTGG AGGAGATTCT
    AATGGATCGG GTATTCCTGG GAAAAAAGGG GAACAAGGGC CTCCGGGGAT AGGACAGCCA
    GGAAAACCAG GTCAGTCAGG ACTTCCTGGA ATTCAGGGAC CACAAGGAAT TAAAGGTGCC
    CAGGGGGAGC CAGGACCACC AGGAGAAGGG AAAAGAGGTC CACAGGGAGA ATCTGGAGAG
    CGTGGTTTGC CTGGGAAAAC AGGGCCACCA GGACCTCAAG GGTCGCCTGG CTTTGTTGGA
    TCCAAAGGGG AAAAGGGAAG CACTGGACAA AAGGGGGCCT CAGGAGCACC TGGAAATTCT
    GGAACAGGTG TCATGGGACC ACCGGGACGG GATGGCACTT CAGGCCAACC AGGAATTCCT
    GGACGTGATG GTGTACCGGG TGAGAGAGGA GAAAAGGGAG ATGCTGGAGT GTGTTCCTGC
    CCACCAGGGC CATGGATGGA TCCAGAAATT TTTGGAAGAC CGGACTCTCC TAATAAGTGG
    AATGGAGATG TCTGGCCTTT TGGAATTCAG GGCCCTCCTG GGCCACCAGG GCCTCCAGGT
    CCCCCAGGAC TTCCTGGTCT ACAGGGACCC CTGGCTGCAG AAAGCAACAT TGATATCCTG
    AAGTGCCGAC CTGAAAAAGA GGATGAAGTG GGAGATGAGC CTGATCATGG CTGTGCAGGT
    GGGCCAGGGC AACCTGGGGC CCCAGGATTA CCAGGACATG ATGGACTACC TGGTCCACGA
    GGACCCCCTG GACTTCCAGG AGCTTCGGGG CCCCCAGGTT TTCCAGGCAC TCTTGGCACC
    CCCGGACTAC CTGGTATGCA GGGAGAAAGA GGACCACCAG GGCCACATGG AGAAAAAGGG
    GAATTGGGAC CACCTGGACT TCCAGGTTAT CCTGGAACTA TGGGATCTCC AGGCCTTCCA
    GGTAGCAAAG GAGAGCGTGG CTACCATGGA CCTGCTGGAG AGAAAGGAGA GTCGGGTCCT
    CCTGGTATTC CGGGTGTTCC TGGTCCTGCT GGCCCTGCTG GACCAAGAGG TGAACGAGGA
    ACTTCAGGGA GCTTCGGAGA AAAAGGTGAC CAGGGATTTT CAGGCCCCCC AGGTTTCCCA
    GGACCTGCGG GACCTCCAGG TTTTCCTGGA AAGACAGGGT CTCCTGGACC ACCTGGGCCT
    ACTGCTGAAA AGGGTTCAGA AGGATTACGT GGTTCTCCGG GACAACCTGG CTCATCAGGC
    CCACCAGGAC CTCCTGGATT ACAGGGGCCG CCTGGGATTG AAGGACTGGA CGGGAAGGAT
    GGGAAACCAG GACTGAGGGG AGAAATGGGA CCTCCTGGGC CTCCTGGGCA TAAGGGTATT
    CCGGGATTCA AAGGGAAAAC AGGGCATCCT GGCTTGCCTG GGCAAAAGGG AGACTGTGGT
    CCTCCAGGTC CTTCAGGCAT TACTGGCAGG CCTGGTGCAG AGGGTGATCC TGGTCCAATG
    GGTCCACCAG GCCGCCCAGG TCCTTCTGGA AACTTGGGAG CTGCAGGACC TACAGGACCA
    CCAGGTAATC CAGGCCCCCC TGGAATGGGA CTTAAAGGTG ATCGAGGACC ACCAGGTGAT
    CGAGGACAAT TGGGTCTTCC GGGTGAGCCA GGTACCCCGG GACACCCAGG ACCTGCTGGT
    GAAATGGGTT CAGATGGTCC TATGGGTAAG GAGGGTCCTT CTGGAAGGCC AGGAGCCCCT
    GGTTCTCCTG GAGAGAAGGG TGAAACCGGA ATGCCTGGAT CTTCAGGCCC CCCAGGAGAA
    AGAGGAAGAC CAGGACCGCC AGGAACATCA GCAGCTTATA CAAAAAGTGA ATCATTAGGG
    ATAGTTGGAC CAAGGGGACC TCCAGGAGAG AGGGGCTCTT CTGGATCTCC AGGACCTGCT
    GGGGCACCAG GAAGCCCAGG ACTTCCAGGA ACACCTGCGG AATCCATCAG TTATGATGAG
    ATTAAGAGAA TGATACGACA AGAGTTGATC AAGATCTTTG ATGAGAGGAT GGCATATTAT
    ACAACCCGCA TGCAGATGCC ATTGGAGATG GCTTCATCAC CAGGAAGACC GGGCTCTCCT
    GGAAAGGATG GATCCCCAGG ACGTCCAGGA CCCCCTGGAT CACCTGGACT GCCAGGACAA
    ATGGGCCGTG AGGGGCGACA AGGTGCCCCG GGAATGAGAG GAGAACCTGG ATCAAAAGGA
    GAAAAGGGGG CAAATGGTAT TGGTGTCATG GGAGATGTTG GACCTCCTGG CTCACCAGGA
    CCACAAGGGC CACCTGGATA TGGCAAACTG GGATTACCAG GACCAATGGG ACAGCAAGGT
    ATACCAGGTA TTCCAGGTTC TCCAGGGGCA AAAGGTTATC CAGGAAAAGA TGGTAGATGT
    AATCCCTCAG ACTGCCACGG TATGATGGGC GTGCAAGAGT ACCACCCAAA GAGCATGAAG
    GGTCCAGGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.