LOC100495765 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100495765
Entrez Gene ID	100495765
Full Name	uncharacterized LOC100495765
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012953686.2	XP_012809140.1	uncharacterized protein LOC100495765 isoform X1
XM_031891164.1	XP_031747024.1	uncharacterized protein LOC100495765 isoform X2
XM_002941147.3	XP_002941193.2	uncharacterized protein LOC100495765 isoform X1
XM_012953687.1	XP_012809141.1	uncharacterized protein LOC100495765 isoform X2
XM_031891162.1	XP_031747022.1	uncharacterized protein LOC100495765 isoform X1
XM_031891163.1	XP_031747023.1	uncharacterized protein LOC100495765 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100495765 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100495765 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa64426	XM_031891162.1 Latest version!	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa64426	XM_031891163.1 Latest version!	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa64427	XM_031891164.1 Latest version!	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa18467	XM_012953686.2 Latest version!	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OXa18467	XM_002941147.3 Latest version!	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OXa36367	XM_012953687.1 Latest version!	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100495765 ( XM_031891162.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747022.1 Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa64426
Clone ID Related Accession (Same CDS sequence)	XM_031891162.1 , XM_031891163.1
Accession Version	XM_031891162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100495765 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGAG CAATATCTGC CGATGAACAG TGCGATGGTC 
CCAGATGAAA GGTTGATGAT GGCCATGGCA ATAATTAAAG ACAAAGATGG GAAAGTCCTA 
GAGCAATCGG TGGCGTCGGT GGCCAAAGGG AGCAAATACC GAGTGAATAA CAGCTATGAT 
AAATACTATG AGCCCAGGCG GAAGGACGTC ATTGTTGCGA TGGCAAAACA ACACGTCGGG 
CAGAAAATGA CTTATAATCT CTGCAAATCC AACTGTGAGC ACTTCGCTAC CCTGATGAGA 
TATGGGGTGG CTTTCTCCAA ACAGGTGGAA GAGTCGAGAG TAGCGCACCT TTTCTGTAAA 
CAAAATGGTC GCTGTTATTG CGATATTGAG GCTTTTCCAC CAAATGACCC AACTCTTCAT 
CTTCTTCAGC AAAACGCCGG CCTGCCCTCC CTGCAGCCAA TGTGCCCTTC TGGCCTACTG 
ACACCACAGA ATTCTTCAGC TCAGCGTTTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747022.1
CDS	104..2539
Translation	MGNSQAKAIPELDLDLSGAELYILSECNELWGRGTVEQLYPEMIRQVSLLSDLIARDPRRVQKVNAAPGDMIEFLGKKSQWAVCTGGGKAAFRTERGLVEEKDIGKIPGGRSYRVNNKFDQKLKPLSGALVMKEARRQLGRRCAEDNDELFVTQLRYGMACSDEDPPPNTGEILCFLFQSQEHWGIYTGDSAVVYLDRGLQEGFSEPSETAYVQEVPLTQLKGVRVINKYDQKLKPKKQGDVAIEAKQKIGERMTWGEKNDTDQLFVTELRYGVKVSDKRPLPEVGDLIEFLQDAKNHWAVYVGDGKAVHRAEHGLVEEDYTGRIAGGHSYRVNNKFDRKLKPRSSTLVVKDVCRQLGKRCAEDSDELFATQLRYGIACSDERPIPEAGDLIEFLRHVKNHWGVYVGDGKVVHRTEFGLVEEDYIGRIAGGRSYRVNNKFDRKLKPRSIALAMTDVRGQLGRRCAEDSDELFVTHLRYGIACSDENPPPDAGEILCYSFQNKEHWGIYTGESTVVYLDRGLQEGFPEPSETAYVQEVPLTQLKGIKVINKYDQMLKPKKQVDIAIEAKQRIGEKVTWGEDDDTDQLFLTELRYGMKVSNKQPPPEPGDLIEFVRLWGAYSHWGVYVGDGNLVHLQPNSKSIFNSLSSFREQYLPMNSAMVPDERLMMAMAIIKDKDGKVLEQSVASVAKGSKYRVNNSYDKYYEPRRKDVIVAMAKQHVGQKMTYNLCKSNCEHFATLMRYGVAFSKQVEESRVAHLFCKQNGRCYCDIEAFPPNDPTLHLLQQNAGLPSLQPMCPSGLLTPQNSSAQRLY

Target ORF information:

RefSeq Version	XM_031891162.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891162.1

ORF Insert Sequence:

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGAG CAATATCTGC CGATGAACAG TGCGATGGTC 
CCAGATGAAA GGTTGATGAT GGCCATGGCA ATAATTAAAG ACAAAGATGG GAAAGTCCTA 
GAGCAATCGG TGGCGTCGGT GGCCAAAGGG AGCAAATACC GAGTGAATAA CAGCTATGAT 
AAATACTATG AGCCCAGGCG GAAGGACGTC ATTGTTGCGA TGGCAAAACA ACACGTCGGG 
CAGAAAATGA CTTATAATCT CTGCAAATCC AACTGTGAGC ACTTCGCTAC CCTGATGAGA 
TATGGGGTGG CTTTCTCCAA ACAGGTGGAA GAGTCGAGAG TAGCGCACCT TTTCTGTAAA 
CAAAATGGTC GCTGTTATTG CGATATTGAG GCTTTTCCAC CAAATGACCC AACTCTTCAT 
CTTCTTCAGC AAAACGCCGG CCTGCCCTCC CTGCAGCCAA TGTGCCCTTC TGGCCTACTG 
ACACCACAGA ATTCTTCAGC TCAGCGTTTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495765 ( XM_031891163.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747023.1 Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa64426
Clone ID Related Accession (Same CDS sequence)	XM_031891162.1 , XM_031891163.1
Accession Version	XM_031891163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100495765 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGAG CAATATCTGC CGATGAACAG TGCGATGGTC 
CCAGATGAAA GGTTGATGAT GGCCATGGCA ATAATTAAAG ACAAAGATGG GAAAGTCCTA 
GAGCAATCGG TGGCGTCGGT GGCCAAAGGG AGCAAATACC GAGTGAATAA CAGCTATGAT 
AAATACTATG AGCCCAGGCG GAAGGACGTC ATTGTTGCGA TGGCAAAACA ACACGTCGGG 
CAGAAAATGA CTTATAATCT CTGCAAATCC AACTGTGAGC ACTTCGCTAC CCTGATGAGA 
TATGGGGTGG CTTTCTCCAA ACAGGTGGAA GAGTCGAGAG TAGCGCACCT TTTCTGTAAA 
CAAAATGGTC GCTGTTATTG CGATATTGAG GCTTTTCCAC CAAATGACCC AACTCTTCAT 
CTTCTTCAGC AAAACGCCGG CCTGCCCTCC CTGCAGCCAA TGTGCCCTTC TGGCCTACTG 
ACACCACAGA ATTCTTCAGC TCAGCGTTTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747023.1
CDS	90..2525
Translation	MGNSQAKAIPELDLDLSGAELYILSECNELWGRGTVEQLYPEMIRQVSLLSDLIARDPRRVQKVNAAPGDMIEFLGKKSQWAVCTGGGKAAFRTERGLVEEKDIGKIPGGRSYRVNNKFDQKLKPLSGALVMKEARRQLGRRCAEDNDELFVTQLRYGMACSDEDPPPNTGEILCFLFQSQEHWGIYTGDSAVVYLDRGLQEGFSEPSETAYVQEVPLTQLKGVRVINKYDQKLKPKKQGDVAIEAKQKIGERMTWGEKNDTDQLFVTELRYGVKVSDKRPLPEVGDLIEFLQDAKNHWAVYVGDGKAVHRAEHGLVEEDYTGRIAGGHSYRVNNKFDRKLKPRSSTLVVKDVCRQLGKRCAEDSDELFATQLRYGIACSDERPIPEAGDLIEFLRHVKNHWGVYVGDGKVVHRTEFGLVEEDYIGRIAGGRSYRVNNKFDRKLKPRSIALAMTDVRGQLGRRCAEDSDELFVTHLRYGIACSDENPPPDAGEILCYSFQNKEHWGIYTGESTVVYLDRGLQEGFPEPSETAYVQEVPLTQLKGIKVINKYDQMLKPKKQVDIAIEAKQRIGEKVTWGEDDDTDQLFLTELRYGMKVSNKQPPPEPGDLIEFVRLWGAYSHWGVYVGDGNLVHLQPNSKSIFNSLSSFREQYLPMNSAMVPDERLMMAMAIIKDKDGKVLEQSVASVAKGSKYRVNNSYDKYYEPRRKDVIVAMAKQHVGQKMTYNLCKSNCEHFATLMRYGVAFSKQVEESRVAHLFCKQNGRCYCDIEAFPPNDPTLHLLQQNAGLPSLQPMCPSGLLTPQNSSAQRLY

Target ORF information:

RefSeq Version	XM_031891163.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891163.1

ORF Insert Sequence:

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGAG CAATATCTGC CGATGAACAG TGCGATGGTC 
CCAGATGAAA GGTTGATGAT GGCCATGGCA ATAATTAAAG ACAAAGATGG GAAAGTCCTA 
GAGCAATCGG TGGCGTCGGT GGCCAAAGGG AGCAAATACC GAGTGAATAA CAGCTATGAT 
AAATACTATG AGCCCAGGCG GAAGGACGTC ATTGTTGCGA TGGCAAAACA ACACGTCGGG 
CAGAAAATGA CTTATAATCT CTGCAAATCC AACTGTGAGC ACTTCGCTAC CCTGATGAGA 
TATGGGGTGG CTTTCTCCAA ACAGGTGGAA GAGTCGAGAG TAGCGCACCT TTTCTGTAAA 
CAAAATGGTC GCTGTTATTG CGATATTGAG GCTTTTCCAC CAAATGACCC AACTCTTCAT 
CTTCTTCAGC AAAACGCCGG CCTGCCCTCC CTGCAGCCAA TGTGCCCTTC TGGCCTACTG 
ACACCACAGA ATTCTTCAGC TCAGCGTTTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495765 ( XM_031891164.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747024.1 Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X3, mRNA.

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CloneID	OXa64427
Clone ID Related Accession (Same CDS sequence)	XM_031891164.1
Accession Version	XM_031891164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100495765 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAATC CGCCCCCTGA CGCCGGAGAG ATACTTTGTT ATTCGTTCCA GAACAAGGAA 
CACTGGGGTA TTTATACAGG AGAGAGCACT GTGGTTTATC TGGACAGAGG TCTGCAGGAA 
GGATTCCCCG AACCTTCAGA AACGGCTTAT GTACAGGAGG TCCCTCTAAC CCAACTGAAG 
GGCATCAAAG TCATCAACAA ATACGATCAG ATGTTAAAGC CTAAAAAGCA AGTAGACATC 
GCCATCGAAG CCAAACAGAG AATTGGGGAG AAGGTGACTT GGGGGGAGGA CGATGACACC 
GATCAGCTGT TTCTAACTGA ACTGAGATAT GGAATGAAGG TCTCAAATAA GCAGCCGCCG 
CCTGAGCCTG GAGACCTGAT TGAGTTTGTC CGCCTGTGGG GGGCCTACAG TCACTGGGGA 
GTCTATGTTG GAGACGGCAA TCTTGTGCAC TTGCAACCCA ATTCCAAATC GATATTTAAC 
TCGCTGTCTT CATTTAGAGA GCAATATCTG CCGATGAACA GTGCGATGGT CCCAGATGAA 
AGGTTGATGA TGGCCATGGC AATAATTAAA GACAAAGATG GGAAAGTCCT AGAGCAATCG 
GTGGCGTCGG TGGCCAAAGG GAGCAAATAC CGAGTGAATA ACAGCTATGA TAAATACTAT 
GAGCCCAGGC GGAAGGACGT CATTGTTGCG ATGGCAAAAC AACACGTCGG GCAGAAAATG 
ACTTATAATC TCTGCAAATC CAACTGTGAG CACTTCGCTA CCCTGATGAG ATATGGGGTG 
GCTTTCTCCA AACAGGTGGA AGAGTCGAGA GTAGCGCACC TTTTCTGTAA ACAAAATGGT 
CGCTGTTATT GCGATATTGA GGCTTTTCCA CCAAATGACC CAACTCTTCA TCTTCTTCAG 
CAAAACGCCG GCCTGCCCTC CCTGCAGCCA ATGTGCCCTT CTGGCCTACT GACACCACAG 
AATTCTTCAG CTCAGCGTTT GTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747024.1
CDS	104..2230
Translation	MGNSQAKAIPELDLDLSGAELYILSECNELWGRGTVEQLYPEMIRQVSLLSDLIARDPRRVQKVNAAPGDMIEFLGKKSQWAVCTGGGKAAFRTERGLVEEKDIGKIPGGRSYRVNNKFDQKLKPLSGALVMKEARRQLGRRCAEDNDELFVTQLRYGMACSDEDPPPNTGEILCFLFQSQEHWGIYTGDSAVVYLDRGLQEGFSEPSETAYVQEVPLTQLKGVRVINKYDQKLKPKKQGDVAIEAKQKIGERMTWGEKNDTDQLFVTELRYGVKVSDKRPLPEVGDLIEFLQDAKNHWAVYVGDGKAVHRAEHGLVEEDYTGRIAGGHSYRVNNKFDRKLKPRSSTLVVKDVCRQLGKRCAEDSDELFATQLRYGIACSDENPPPDAGEILCYSFQNKEHWGIYTGESTVVYLDRGLQEGFPEPSETAYVQEVPLTQLKGIKVINKYDQMLKPKKQVDIAIEAKQRIGEKVTWGEDDDTDQLFLTELRYGMKVSNKQPPPEPGDLIEFVRLWGAYSHWGVYVGDGNLVHLQPNSKSIFNSLSSFREQYLPMNSAMVPDERLMMAMAIIKDKDGKVLEQSVASVAKGSKYRVNNSYDKYYEPRRKDVIVAMAKQHVGQKMTYNLCKSNCEHFATLMRYGVAFSKQVEESRVAHLFCKQNGRCYCDIEAFPPNDPTLHLLQQNAGLPSLQPMCPSGLLTPQNSSAQRLY

Target ORF information:

RefSeq Version	XM_031891164.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891164.1

ORF Insert Sequence:

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAATC CGCCCCCTGA CGCCGGAGAG ATACTTTGTT ATTCGTTCCA GAACAAGGAA 
CACTGGGGTA TTTATACAGG AGAGAGCACT GTGGTTTATC TGGACAGAGG TCTGCAGGAA 
GGATTCCCCG AACCTTCAGA AACGGCTTAT GTACAGGAGG TCCCTCTAAC CCAACTGAAG 
GGCATCAAAG TCATCAACAA ATACGATCAG ATGTTAAAGC CTAAAAAGCA AGTAGACATC 
GCCATCGAAG CCAAACAGAG AATTGGGGAG AAGGTGACTT GGGGGGAGGA CGATGACACC 
GATCAGCTGT TTCTAACTGA ACTGAGATAT GGAATGAAGG TCTCAAATAA GCAGCCGCCG 
CCTGAGCCTG GAGACCTGAT TGAGTTTGTC CGCCTGTGGG GGGCCTACAG TCACTGGGGA 
GTCTATGTTG GAGACGGCAA TCTTGTGCAC TTGCAACCCA ATTCCAAATC GATATTTAAC 
TCGCTGTCTT CATTTAGAGA GCAATATCTG CCGATGAACA GTGCGATGGT CCCAGATGAA 
AGGTTGATGA TGGCCATGGC AATAATTAAA GACAAAGATG GGAAAGTCCT AGAGCAATCG 
GTGGCGTCGG TGGCCAAAGG GAGCAAATAC CGAGTGAATA ACAGCTATGA TAAATACTAT 
GAGCCCAGGC GGAAGGACGT CATTGTTGCG ATGGCAAAAC AACACGTCGG GCAGAAAATG 
ACTTATAATC TCTGCAAATC CAACTGTGAG CACTTCGCTA CCCTGATGAG ATATGGGGTG 
GCTTTCTCCA AACAGGTGGA AGAGTCGAGA GTAGCGCACC TTTTCTGTAA ACAAAATGGT 
CGCTGTTATT GCGATATTGA GGCTTTTCCA CCAAATGACC CAACTCTTCA TCTTCTTCAG 
CAAAACGCCG GCCTGCCCTC CCTGCAGCCA ATGTGCCCTT CTGGCCTACT GACACCACAG 
AATTCTTCAG CTCAGCGTTT GTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495765 ( XM_012953686.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809140.1 Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X2, mRNA.

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CloneID	OXa18467
Clone ID Related Accession (Same CDS sequence)	XM_012953686.2 , XM_002941147.3
Accession Version	XM_012953686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100495765 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683437.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGGT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809140.1
CDS	95..2050
Translation	MGNSQAKAIPELDLDLSGAELYILSECNELWGRGTVEQLYPEMIRQVSLLSDLIARDPRRVQKVNAAPGDMIEFLGKKSQWAVCTGGGKAAFRTERGLVEEKDIGKIPGGRSYRVNNKFDQKLKPLSGALVMKEARRQLGRRCAEDNDELFVTQLRYGMACSDEDPPPNTGEILCFLFQSQEHWGIYTGDSAVVYLDRGLQEGFSEPSETAYVQEVPLTQLKGVRVINKYDQKLKPKKQGDVAIEAKQKIGERMTWGEKNDTDQLFVTELRYGVKVSDKRPLPEVGDLIEFLQDAKNHWAVYVGDGKAVHRAEHGLVEEDYTGRIAGGHSYRVNNKFDRKLKPRSSTLVVKDVCRQLGKRCAEDSDELFATQLRYGIACSDERPIPEAGDLIEFLRHVKNHWGVYVGDGKVVHRTEFGLVEEDYIGRIAGGRSYRVNNKFDRKLKPRSIALAMTDVRGQLGRRCAEDSDELFVTHLRYGIACSDENPPPDAGEILCYSFQNKEHWGIYTGESTVVYLDRGLQEGFPEPSETAYVQEVPLTQLKGIKVINKYDQMLKPKKQVDIAIEAKQRIGEKVTWGEDDDTDQLFLTELRYGMKVSNKQPPPEPGDLIEFVRLWGAYSHWGVYVGDGNLVHLQPNSKSIFNSLSSFRGK

Target ORF information:

RefSeq Version	XM_012953686.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953686.2

ORF Insert Sequence:

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGGT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495765 ( XM_002941147.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941193.2 Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X1, mRNA.

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CloneID	OXa18467
Clone ID Related Accession (Same CDS sequence)	XM_012953686.2 , XM_002941147.3
Accession Version	XM_002941147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100495765 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683437.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002941147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGGT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941193.2
CDS	106..2061
Translation	MGNSQAKAIPELDLDLSGAELYILSECNELWGRGTVEQLYPEMIRQVSLLSDLIARDPRRVQKVNAAPGDMIEFLGKKSQWAVCTGGGKAAFRTERGLVEEKDIGKIPGGRSYRVNNKFDQKLKPLSGALVMKEARRQLGRRCAEDNDELFVTQLRYGMACSDEDPPPNTGEILCFLFQSQEHWGIYTGDSAVVYLDRGLQEGFSEPSETAYVQEVPLTQLKGVRVINKYDQKLKPKKQGDVAIEAKQKIGERMTWGEKNDTDQLFVTELRYGVKVSDKRPLPEVGDLIEFLQDAKNHWAVYVGDGKAVHRAEHGLVEEDYTGRIAGGHSYRVNNKFDRKLKPRSSTLVVKDVCRQLGKRCAEDSDELFATQLRYGIACSDERPIPEAGDLIEFLRHVKNHWGVYVGDGKVVHRTEFGLVEEDYIGRIAGGRSYRVNNKFDRKLKPRSIALAMTDVRGQLGRRCAEDSDELFVTHLRYGIACSDENPPPDAGEILCYSFQNKEHWGIYTGESTVVYLDRGLQEGFPEPSETAYVQEVPLTQLKGIKVINKYDQMLKPKKQVDIAIEAKQRIGEKVTWGEDDDTDQLFLTELRYGMKVSNKQPPPEPGDLIEFVRLWGAYSHWGVYVGDGNLVHLQPNSKSIFNSLSSFRGK

Target ORF information:

RefSeq Version	XM_002941147.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941147.3

ORF Insert Sequence:

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAGGC CGATCCCTGA AGCTGGAGAC CTGATTGAGT TTCTTCGTCA TGTCAAAAAC 
CACTGGGGGG TCTACGTTGG AGATGGCAAG GTTGTACACA GGACGGAGTT CGGATTGGTG 
GAGGAAGATT ACATTGGGAG AATCGCGGGC GGACGTTCCT ATAGAGTCAA TAACAAGTTT 
GACCGGAAAC TGAAACCTCG CTCCATCGCT TTAGCGATGA CGGATGTACG CGGCCAGCTT 
GGGAGGAGAT GTGCAGAAGA TAGTGATGAA CTGTTTGTCA CACACCTGAG ATATGGAATT 
GCCTGCTCAG ATGAGAATCC GCCCCCTGAC GCCGGAGAGA TACTTTGTTA TTCGTTCCAG 
AACAAGGAAC ACTGGGGTAT TTATACAGGA GAGAGCACTG TGGTTTATCT GGACAGAGGT 
CTGCAGGAAG GATTCCCCGA ACCTTCAGAA ACGGCTTATG TACAGGAGGT CCCTCTAACC 
CAACTGAAGG GCATCAAAGT CATCAACAAA TACGATCAGA TGTTAAAGCC TAAAAAGCAA 
GTAGACATCG CCATCGAAGC CAAACAGAGA ATTGGGGAGA AGGTGACTTG GGGGGAGGAC 
GATGACACCG ATCAGCTGTT TCTAACTGAA CTGAGATATG GAATGAAGGT CTCAAATAAG 
CAGCCGCCGC CTGAGCCTGG AGACCTGATT GAGTTTGTCC GCCTGTGGGG GGCCTACAGT 
CACTGGGGAG TCTATGTTGG AGACGGCAAT CTTGTGCACT TGCAACCCAA TTCCAAATCG 
ATATTTAACT CGCTGTCTTC ATTTAGAGGT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495765 ( XM_012953687.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809141.1 Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X3, mRNA.

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CloneID	OXa36367
Clone ID Related Accession (Same CDS sequence)	XM_012953687.1
Accession Version	XM_012953687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100495765 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683437.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAATC CGCCCCCTGA CGCCGGAGAG ATACTTTGTT ATTCGTTCCA GAACAAGGAA 
CACTGGGGTA TTTATACAGG AGAGAGCACT GTGGTTTATC TGGACAGAGG TCTGCAGGAA 
GGATTCCCCG AACCTTCAGA AACGGCTTAT GTACAGGAGG TCCCTCTAAC CCAACTGAAG 
GGCATCAAAG TCATCAACAA ATACGATCAG ATGTTAAAGC CTAAAAAGCA AGTAGACATC 
GCCATCGAAG CCAAACAGAG AATTGGGGAG AAGGTGACTT GGGGGGAGGA CGATGACACC 
GATCAGCTGT TTCTAACTGA ACTGAGATAT GGAATGAAGG TCTCAAATAA GCAGCCGCCG 
CCTGAGCCTG GAGACCTGAT TGAGTTTGTC CGCCTGTGGG GGGCCTACAG TCACTGGGGA 
GTCTATGTTG GAGACGGCAA TCTTGTGCAC TTGCAACCCA ATTCCAAATC GATATTTAAC 
TCGCTGTCTT CATTTAGAGG TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809141.1
CDS	106..1752
Translation	MGNSQAKAIPELDLDLSGAELYILSECNELWGRGTVEQLYPEMIRQVSLLSDLIARDPRRVQKVNAAPGDMIEFLGKKSQWAVCTGGGKAAFRTERGLVEEKDIGKIPGGRSYRVNNKFDQKLKPLSGALVMKEARRQLGRRCAEDNDELFVTQLRYGMACSDEDPPPNTGEILCFLFQSQEHWGIYTGDSAVVYLDRGLQEGFSEPSETAYVQEVPLTQLKGVRVINKYDQKLKPKKQGDVAIEAKQKIGERMTWGEKNDTDQLFVTELRYGVKVSDKRPLPEVGDLIEFLQDAKNHWAVYVGDGKAVHRAEHGLVEEDYTGRIAGGHSYRVNNKFDRKLKPRSSTLVVKDVCRQLGKRCAEDSDELFATQLRYGIACSDENPPPDAGEILCYSFQNKEHWGIYTGESTVVYLDRGLQEGFPEPSETAYVQEVPLTQLKGIKVINKYDQMLKPKKQVDIAIEAKQRIGEKVTWGEDDDTDQLFLTELRYGMKVSNKQPPPEPGDLIEFVRLWGAYSHWGVYVGDGNLVHLQPNSKSIFNSLSSFRGK

Target ORF information:

RefSeq Version	XM_012953687.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100495765 (LOC100495765), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953687.1

ORF Insert Sequence:

ATGGGGAATT CACAGGCAAA AGCCATCCCA GAGCTGGACC TGGACCTGTC AGGGGCAGAG 
TTATATATCC TGTCAGAGTG CAATGAACTG TGGGGGAGAG GCACGGTGGA ACAGCTGTAC 
CCCGAAATGA TTAGACAGGT GTCGCTGTTA TCAGACTTGA TCGCCCGAGA CCCGAGAAGA 
GTCCAGAAAG TAAATGCTGC CCCAGGAGAC ATGATTGAAT TTCTGGGGAA GAAATCCCAA 
TGGGCAGTTT GTACCGGAGG GGGCAAAGCG GCGTTTAGAA CAGAGCGTGG ACTGGTGGAG 
GAAAAGGACA TCGGGAAAAT CCCGGGAGGC CGTTCCTATC GAGTCAATAA TAAATTTGAC 
CAGAAACTAA AACCTCTCTC CGGCGCTTTA GTGATGAAGG AGGCGCGCAG ACAGCTTGGG 
AGGAGATGTG CAGAAGATAA TGATGAACTG TTTGTTACAC AACTCAGATA TGGAATGGCC 
TGTTCAGATG AGGATCCTCC TCCTAACACC GGAGAGATAC TTTGTTTTTT ATTCCAAAGC 
CAGGAACACT GGGGTATTTA TACAGGAGAC AGCGCTGTGG TTTATCTCGA CAGAGGTCTG 
CAGGAAGGAT TCTCTGAACC TTCAGAAACG GCTTATGTAC AGGAGGTCCC TCTAACCCAA 
CTGAAGGGCG TCAGAGTCAT CAACAAATAT GATCAGAAAT TAAAGCCCAA AAAACAAGGA 
GATGTGGCCA TAGAAGCCAA GCAAAAAATT GGGGAGAGAA TGACTTGGGG GGAGAAGAAC 
GACACCGATC AGCTGTTTGT AACTGAACTG AGATATGGAG TGAAGGTCTC CGATAAGCGC 
CCCCTGCCTG AGGTTGGAGA CCTGATTGAG TTTCTCCAAG ATGCCAAAAA CCATTGGGCA 
GTCTATGTGG GAGACGGCAA GGCTGTGCAC AGGGCGGAGC ATGGATTGGT GGAGGAAGAT 
TACACTGGGA GAATCGCGGG TGGCCATTCC TATAGAGTTA ATAATAAATT TGACCGGAAA 
CTGAAACCTC GTTCCAGCAC GTTAGTGGTC AAGGATGTTT GCAGGCAGCT TGGGAAGAGA 
TGTGCAGAAG ATAGTGATGA ACTGTTTGCT ACACAACTCA GATATGGAAT TGCCTGCTCA 
GATGAGAATC CGCCCCCTGA CGCCGGAGAG ATACTTTGTT ATTCGTTCCA GAACAAGGAA 
CACTGGGGTA TTTATACAGG AGAGAGCACT GTGGTTTATC TGGACAGAGG TCTGCAGGAA 
GGATTCCCCG AACCTTCAGA AACGGCTTAT GTACAGGAGG TCCCTCTAAC CCAACTGAAG 
GGCATCAAAG TCATCAACAA ATACGATCAG ATGTTAAAGC CTAAAAAGCA AGTAGACATC 
GCCATCGAAG CCAAACAGAG AATTGGGGAG AAGGTGACTT GGGGGGAGGA CGATGACACC 
GATCAGCTGT TTCTAACTGA ACTGAGATAT GGAATGAAGG TCTCAAATAA GCAGCCGCCG 
CCTGAGCCTG GAGACCTGAT TGAGTTTGTC CGCCTGTGGG GGGCCTACAG TCACTGGGGA 
GTCTATGTTG GAGACGGCAA TCTTGTGCAC TTGCAACCCA ATTCCAAATC GATATTTAAC 
TCGCTGTCTT CATTTAGAGG TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100495765 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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