spag5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following spag5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spag5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa52067 XM_012957454.3
Latest version!
Xenopus tropicalis sperm associated antigen 5 (spag5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa36065 XM_012957454.2
Latest version!
Xenopus tropicalis sperm associated antigen 5 (spag5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa52067
    Clone ID Related Accession (Same CDS sequence) XM_012957454.3
    Accession Version XM_012957454.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm-associated antigen 5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGCCAC TGAAAGCACA AATTTCTGAT GAAAACAGGA TTACGACAAT GCAGACTGCA 
    CAACGCAAAT CTGTTGGGAG GACTCCGCTC AAAGATTTGA CAGCTCAGTC TTTTACATTG
    TCCGAGCAAA ATAGTTTGGT TAAAACAAGG TTAAATTCAA ACAAATCCCC AAGCCTCACA
    GTGAACAAAC AGCACTTCAA GGAAAATAAG AGTGGCAACA CTGGAACATC ACCAGTTAGC
    ATATGGGTGG AATCTGAGAC ACAAGTTCCT CTTATGGACA ACACCATAAG TGAAGTGTGT
    GCTTCAAAGC GTTTAAAACC AATTACCCTT TTTGAGACAA AGTTTACTAC ATTGAACTCA
    GATGGATTAG CTCCCAAAAT GAAAAGTAAA GATTCTAGCA ATATACATGA GTGTTTGCAG
    CCTTCAGAAC CTGCAACTTC TGAAAAGATA CTGTGCTTAT CTGCCTGTGG TCAAGAAAAG
    TTTCCCCTCA ATTGCTTGGA CAGTGACTCT TCTTTTCCCA GTAATGCAGA TCCTTGCATG
    AATGTGACAG CAAGCCTGCC TGTGGAAACA CAAATTAATG AACAGGAGTC TAGAAAAGAC
    AGGGATCAAA AGTTAGAAGC AGACACATGC CCAGTGACTG TTCCACTCTC TCACAAATCA
    GTGGTTGTTT CTCAATGTTC TAGCCAGGCA TTGGGTGAAA CAAGGAAGAG TCTTGAATTA
    CAATTGTGTG CAAATGAAGC TCTAAAAGAA TCGCCCTTTG GCATAAGCCT AACTATAAAT
    GAAGCATTTT CTCATGCTGC ATCCACATTC TGCATTCCCG AACAAAATGA TGTTGCTGTG
    TCACACCAAG GAATTATTGA TCCATCTTTA AGTAACCACT TTAATGAGAG TGCAAATGGA
    AATAACTGTC CCAACAAGAC TGAAGAAGTC CAGTTATTGT TGAGCCTAAA AGAGGAGGAG
    AGAGTCCCAA ATGCCTCAGA GCTTGAATCT TGCAAAATGT TAAATGGACT TGCTAATGAC
    ACATATTTAA TTGATACTTC ATCAGCAGCT TCACCTGAGC ATGTTGACAA ACACAGCTTT
    CCAGCATTGA CTACAAATTT AATTTCCACT GAGTATTCTG AACAGGATGG CCTTGACTGC
    TCTCAAATCA TATCAACTTT AGATAATACA CAACCTGAAG GCTATTATAA TAAGGCGGAT
    AAGACTGACG TCAACAAACC ACTGAAAGGT GATGACAGTG TTCTGCCTAT GTCCCATAAA
    ACTAAAGAAA GTCCTGCAAC TTATCCTACA ACATATATCC CATTACAGCA ACTAGCTGAT
    AGTTCTTCTA AAATATTTCT TCATTCCTTC ATTCAAGAGG AAGTCTGTAA TAAGACTTTT
    GATCTAGACT GCATTACAAT AAAGGATAAG AAAGTAGACT TTAAATGTAT TGAACCAAAC
    AGTATTATAC ATACTAGGAG TTGTGATGAG ACTGATGATC TATTGGTAGG GTGTAAACTG
    CTACCAGAGC AGACTGGGGG CACCTTAAAA ATAAAAGATA TTGCAGATCG TGGCACTGAT
    TACAAGACAG AGTTAGAATC CTCCATTGTT TTAGAGGAAG AAATGGCTTC TAAGAACTAT
    GATGGTTCTC TTAAAAATGA AAATGTCAAA GTACGTCCTT CCATTGTTGT GCAAAGTGAT
    ACTGGGCAAG CAGCTGTGCC GCTTGTTGCT TCTGCCAATG AAAGCAGGTC TGATTCAGTA
    GCAAACCCAA GGGACTCTGT TGTAAATAAC CAGCTACAAG CAAGGAATTT TGATATTCCA
    AAATTATACA AAAGTGTACT CAGCAAGATG TCTCCTAAAG GGGAAAGCTC TAAATTGGAA
    GTTGGAACGT GTTCGACCCC AACTTCAACC TCAAATACAT CCACATGGAC AACACCTATA
    ATGTTGCTGA ACAAGAGCTT GAATACATCA TGGGACTTTG AAAAGGGGAG GTCAAGTCCA
    AGAGACAATG CATCGGAGAC AGATTCCTTG CTCTGGAACT TCTCCAGGGA GTCCTTTAAT
    AAAGCATCAC GTGAGGAGTT GATGGATCGT TTAGAGAGTA CTTTGATAGT TGTTGAAGTT
    CTTTCACGAC AGCTTCAAGG CTGGCAGCAG AATCGAATTG GTTCTAGACC ATCTGAACAG
    AGAGACTCTG CCACACAAAC ATGTGTAACA TACACAAGTA CGGAAGAGCA ATATTATCAC
    AACTTGTATG TAGAAACCAT GGAAAGACTT CAAAACATGC AGCGATGCAA TGACGTAGAA
    AAGAAACTGC AGGAGATACT TAAAAGGAGT GTTGATGTGC TGATATCTAA CAAGACTCAA
    TCTATGTCAT TGATTGAGTT TGCTGAGAAC ATGCAAGACA AAACTCAGGA GGATAAAGCA
    GATATTAGCA GGAAGATGTG CCAAGCCAGG TCACTTATAG CTGATCACAT GACTTTATTG
    AATAAAATGA ACAAGAAGAT GCAAGCAAAC CAGCACCAAA TGGCCGAAAT GAGAACTAGT
    ATGGAAAAGG CAGTTGGTGA TAAAGAAGCG GCTGATCAGT GTTTGAAAGA TCTTGAGACC
    CATTCTTCTA CAGTCATAGC TCAGCTGCAA CGTGACTTGG AATCTGAGCA ATTGCTTTGT
    GATGCTGTGA AAAAAGCTTA TACACAACAG CTCTCCTTTA ATGAAGAATT GGTTGACTTT
    GCTAAAAAGG CTCAGTCTGT CTGCTCAGAA ATGGAAGAGG ATAGAGTCCA GTTGCAAGTA
    AAGTGTTCTC AGGCCAGGGA GCTCATGTCA AAACACTGGG TCCTGTTTGA CATGATGAAA
    AATAAAACAG ATCATGCTGT GCATAAGTTT GAATCCATGA AAAGTGAGCG GGATGCGGCT
    TACTTGGAAA AAAATGAAAT GTGCAATCAG TTAGAGGATG TGAACTCACA GAAGGCTCAG
    CTGGAAACAG GAATAGCTCA CCTTAGTTCA GAGCTGGAGT CCCTGATGGA ACAGATATGT
    AAACTTAAGT CTGAAAATGA TCACCTGAAA CTGGAAAACT CTGAGCAGAC GGAGGAACTG
    TGTGCTAAAG ATTCGTCTAT GAAACTGCTA GAAAAAGAGC TTAATGAGAC AACCATTCGG
    GAACAAGAAA TTAAAGAACA CAACAAGAAA TTGTCAGCTG AAACTGTGCC GAGGTTGGAG
    CAGAAGCTGT TAGAGGCATT ACAAGAGAAA GAGGTTCTAC AAATAAAGAT ACAAGAGTTC
    CAAAAGCAGC ACGCATCCCA GACTGCATCT TATAAGGAGA GCATTGAGTT CCTTGAGCAA
    GAGAACCATG TGTGGTTTGA GCAGGTCGCA GAAACGGAGT CACAGCTAAA GAATAATCTC
    TTTGCCTTAC GTGAGAGAAA CCTGCAGTGT GAGACCCAAA AGGACAGAAT AAGCGAACTA
    CAGAGTGAAC TGTTAAAACT GAAGGAAGAA TTTCAGATGA CAAAGGAGGC AGCAAACGAT
    ACGCTGCTGA AAATGGGTAA AGAGATATCG GATTCATCCA CAGAAGCCGG CAAAATCAGG
    GACAACCTGC TGTGTGTGAA AGAACAAATA ATGGACACAT TACATGCAGA GGTAGCAGGT
    GCTTCCCAAA TGCTAGAGAC TCCTGCCAGG GGACTTGGGA GTTGCCCCTC TACTTACATG
    ATCAATAGCA CCCTAAAGTC TCAAACTAAG CAATCTAAAA CAGCCAAGAA TGCCGAAAGC
    ATTTGGAGTG AGACAAGTGC CTTTACCATT GTTCAACCAG TGGCCTCGCC CACTACAGAT
    AACAAGGAGG AAACACTTCC AGATGTAATA TGTGAATTAG GTGAAATTGT GTCAAACTTT
    GCTGTCTCCT CTTCTTATGT CATTGGAGTG AAGGAACAAG AAATCGAAGA CTTTAAGAGA
    GAAATATCGG ACCTAAAGGA TGAGCTACAG AATATGCACT TCAAAAACAC AATTGAAAAA
    CGTGATTTAA TGGAAGAGAT TGGAATTCTG AAAAGAAAAA ATAGGAATCT GGAGGACTGT
    GTTAACAGTA AGCAGCAGTG TGTTCAAGAG CTTGAGGAAA TTGTAAATCA ACAGGAACAG
    AGGTTACTAC AGCAAGTTAG CAAGGAGAAA GAACGAGAGG ACATGATGCA AGAAAATGCA
    ACATTAAAGC GATCCCTACA GCAGTGTGAA AGTGAATTAC TAGTCCTTAA AGATGAGCTG
    TCAAAGAACC CAACTGAAGC AGCTAGAGAC TGGATGCAGG AGAAGTTACT TTTACATAAG
    GATTTGCGAA AGCTAAGACT GATGCTTGTG GAAACTGAAA ATTCTAAGTC TGAAATTCTA
    CATCGAGCAA TGAGACATAG GAATATCTTG GAAGGAAATC TCGCCCGCTC TGAGCTGGAG
    TTAAAGAAGC TGGATGATAC TATTGAGAAA ATAAGAAAGA CTCTCTTGTC TATACCTGAA
    GTTGTCTCCA ACTGTGAAGA ACTGAAACAA ATGCTGGAAT ATCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812908.2
    CDS122..4612
    Translation

    Target ORF information:

    RefSeq Version XM_012957454.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm associated antigen 5 (spag5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957454.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGCCAC TGAAAGCACA AATTTCTGAT GAAAACAGGA TTACGACAAT GCAGACTGCA 
    CAACGCAAAT CTGTTGGGAG GACTCCGCTC AAAGATTTGA CAGCTCAGTC TTTTACATTG
    TCCGAGCAAA ATAGTTTGGT TAAAACAAGG TTAAATTCAA ACAAATCCCC AAGCCTCACA
    GTGAACAAAC AGCACTTCAA GGAAAATAAG AGTGGCAACA CTGGAACATC ACCAGTTAGC
    ATATGGGTGG AATCTGAGAC ACAAGTTCCT CTTATGGACA ACACCATAAG TGAAGTGTGT
    GCTTCAAAGC GTTTAAAACC AATTACCCTT TTTGAGACAA AGTTTACTAC ATTGAACTCA
    GATGGATTAG CTCCCAAAAT GAAAAGTAAA GATTCTAGCA ATATACATGA GTGTTTGCAG
    CCTTCAGAAC CTGCAACTTC TGAAAAGATA CTGTGCTTAT CTGCCTGTGG TCAAGAAAAG
    TTTCCCCTCA ATTGCTTGGA CAGTGACTCT TCTTTTCCCA GTAATGCAGA TCCTTGCATG
    AATGTGACAG CAAGCCTGCC TGTGGAAACA CAAATTAATG AACAGGAGTC TAGAAAAGAC
    AGGGATCAAA AGTTAGAAGC AGACACATGC CCAGTGACTG TTCCACTCTC TCACAAATCA
    GTGGTTGTTT CTCAATGTTC TAGCCAGGCA TTGGGTGAAA CAAGGAAGAG TCTTGAATTA
    CAATTGTGTG CAAATGAAGC TCTAAAAGAA TCGCCCTTTG GCATAAGCCT AACTATAAAT
    GAAGCATTTT CTCATGCTGC ATCCACATTC TGCATTCCCG AACAAAATGA TGTTGCTGTG
    TCACACCAAG GAATTATTGA TCCATCTTTA AGTAACCACT TTAATGAGAG TGCAAATGGA
    AATAACTGTC CCAACAAGAC TGAAGAAGTC CAGTTATTGT TGAGCCTAAA AGAGGAGGAG
    AGAGTCCCAA ATGCCTCAGA GCTTGAATCT TGCAAAATGT TAAATGGACT TGCTAATGAC
    ACATATTTAA TTGATACTTC ATCAGCAGCT TCACCTGAGC ATGTTGACAA ACACAGCTTT
    CCAGCATTGA CTACAAATTT AATTTCCACT GAGTATTCTG AACAGGATGG CCTTGACTGC
    TCTCAAATCA TATCAACTTT AGATAATACA CAACCTGAAG GCTATTATAA TAAGGCGGAT
    AAGACTGACG TCAACAAACC ACTGAAAGGT GATGACAGTG TTCTGCCTAT GTCCCATAAA
    ACTAAAGAAA GTCCTGCAAC TTATCCTACA ACATATATCC CATTACAGCA ACTAGCTGAT
    AGTTCTTCTA AAATATTTCT TCATTCCTTC ATTCAAGAGG AAGTCTGTAA TAAGACTTTT
    GATCTAGACT GCATTACAAT AAAGGATAAG AAAGTAGACT TTAAATGTAT TGAACCAAAC
    AGTATTATAC ATACTAGGAG TTGTGATGAG ACTGATGATC TATTGGTAGG GTGTAAACTG
    CTACCAGAGC AGACTGGGGG CACCTTAAAA ATAAAAGATA TTGCAGATCG TGGCACTGAT
    TACAAGACAG AGTTAGAATC CTCCATTGTT TTAGAGGAAG AAATGGCTTC TAAGAACTAT
    GATGGTTCTC TTAAAAATGA AAATGTCAAA GTACGTCCTT CCATTGTTGT GCAAAGTGAT
    ACTGGGCAAG CAGCTGTGCC GCTTGTTGCT TCTGCCAATG AAAGCAGGTC TGATTCAGTA
    GCAAACCCAA GGGACTCTGT TGTAAATAAC CAGCTACAAG CAAGGAATTT TGATATTCCA
    AAATTATACA AAAGTGTACT CAGCAAGATG TCTCCTAAAG GGGAAAGCTC TAAATTGGAA
    GTTGGAACGT GTTCGACCCC AACTTCAACC TCAAATACAT CCACATGGAC AACACCTATA
    ATGTTGCTGA ACAAGAGCTT GAATACATCA TGGGACTTTG AAAAGGGGAG GTCAAGTCCA
    AGAGACAATG CATCGGAGAC AGATTCCTTG CTCTGGAACT TCTCCAGGGA GTCCTTTAAT
    AAAGCATCAC GTGAGGAGTT GATGGATCGT TTAGAGAGTA CTTTGATAGT TGTTGAAGTT
    CTTTCACGAC AGCTTCAAGG CTGGCAGCAG AATCGAATTG GTTCTAGACC ATCTGAACAG
    AGAGACTCTG CCACACAAAC ATGTGTAACA TACACAAGTA CGGAAGAGCA ATATTATCAC
    AACTTGTATG TAGAAACCAT GGAAAGACTT CAAAACATGC AGCGATGCAA TGACGTAGAA
    AAGAAACTGC AGGAGATACT TAAAAGGAGT GTTGATGTGC TGATATCTAA CAAGACTCAA
    TCTATGTCAT TGATTGAGTT TGCTGAGAAC ATGCAAGACA AAACTCAGGA GGATAAAGCA
    GATATTAGCA GGAAGATGTG CCAAGCCAGG TCACTTATAG CTGATCACAT GACTTTATTG
    AATAAAATGA ACAAGAAGAT GCAAGCAAAC CAGCACCAAA TGGCCGAAAT GAGAACTAGT
    ATGGAAAAGG CAGTTGGTGA TAAAGAAGCG GCTGATCAGT GTTTGAAAGA TCTTGAGACC
    CATTCTTCTA CAGTCATAGC TCAGCTGCAA CGTGACTTGG AATCTGAGCA ATTGCTTTGT
    GATGCTGTGA AAAAAGCTTA TACACAACAG CTCTCCTTTA ATGAAGAATT GGTTGACTTT
    GCTAAAAAGG CTCAGTCTGT CTGCTCAGAA ATGGAAGAGG ATAGAGTCCA GTTGCAAGTA
    AAGTGTTCTC AGGCCAGGGA GCTCATGTCA AAACACTGGG TCCTGTTTGA CATGATGAAA
    AATAAAACAG ATCATGCTGT GCATAAGTTT GAATCCATGA AAAGTGAGCG GGATGCGGCT
    TACTTGGAAA AAAATGAAAT GTGCAATCAG TTAGAGGATG TGAACTCACA GAAGGCTCAG
    CTGGAAACAG GAATAGCTCA CCTTAGTTCA GAGCTGGAGT CCCTGATGGA ACAGATATGT
    AAACTTAAGT CTGAAAATGA TCACCTGAAA CTGGAAAACT CTGAGCAGAC GGAGGAACTG
    TGTGCTAAAG ATTCGTCTAT GAAACTGCTA GAAAAAGAGC TTAATGAGAC AACCATTCGG
    GAACAAGAAA TTAAAGAACA CAACAAGAAA TTGTCAGCTG AAACTGTGCC GAGGTTGGAG
    CAGAAGCTGT TAGAGGCATT ACAAGAGAAA GAGGTTCTAC AAATAAAGAT ACAAGAGTTC
    CAAAAGCAGC ACGCATCCCA GACTGCATCT TATAAGGAGA GCATTGAGTT CCTTGAGCAA
    GAGAACCATG TGTGGTTTGA GCAGGTCGCA GAAACGGAGT CACAGCTAAA GAATAATCTC
    TTTGCCTTAC GTGAGAGAAA CCTGCAGTGT GAGACCCAAA AGGACAGAAT AAGCGAACTA
    CAGAGTGAAC TGTTAAAACT GAAGGAAGAA TTTCAGATGA CAAAGGAGGC AGCAAACGAT
    ACGCTGCTGA AAATGGGTAA AGAGATATCG GATTCATCCA CAGAAGCCGG CAAAATCAGG
    GACAACCTGC TGTGTGTGAA AGAACAAATA ATGGACACAT TACATGCAGA GGTAGCAGGT
    GCTTCCCAAA TGCTAGAGAC TCCTGCCAGG GGACTTGGGA GTTGCCCCTC TACTTACATG
    ATCAATAGCA CCCTAAAGTC TCAAACTAAG CAATCTAAAA CAGCCAAGAA TGCCGAAAGC
    ATTTGGAGTG AGACAAGTGC CTTTACCATT GTTCAACCAG TGGCCTCGCC CACTACAGAT
    AACAAGGAGG AAACACTTCC AGATGTAATA TGTGAATTAG GTGAAATTGT GTCAAACTTT
    GCTGTCTCCT CTTCTTATGT CATTGGAGTG AAGGAACAAG AAATCGAAGA CTTTAAGAGA
    GAAATATCGG ACCTAAAGGA TGAGCTACAG AATATGCACT TCAAAAACAC AATTGAAAAA
    CGTGATTTAA TGGAAGAGAT TGGAATTCTG AAAAGAAAAA ATAGGAATCT GGAGGACTGT
    GTTAACAGTA AGCAGCAGTG TGTTCAAGAG CTTGAGGAAA TTGTAAATCA ACAGGAACAG
    AGGTTACTAC AGCAAGTTAG CAAGGAGAAA GAACGAGAGG ACATGATGCA AGAAAATGCA
    ACATTAAAGC GATCCCTACA GCAGTGTGAA AGTGAATTAC TAGTCCTTAA AGATGAGCTG
    TCAAAGAACC CAACTGAAGC AGCTAGAGAC TGGATGCAGG AGAAGTTACT TTTACATAAG
    GATTTGCGAA AGCTAAGACT GATGCTTGTG GAAACTGAAA ATTCTAAGTC TGAAATTCTA
    CATCGAGCAA TGAGACATAG GAATATCTTG GAAGGAAATC TCGCCCGCTC TGAGCTGGAG
    TTAAAGAAGC TGGATGATAC TATTGAGAAA ATAAGAAAGA CTCTCTTGTC TATACCTGAA
    GTTGTCTCCA ACTGTGAAGA ACTGAAACAA ATGCTGGAAT ATCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36065
    Clone ID Related Accession (Same CDS sequence) XM_012957454.2
    Accession Version XM_012957454.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm-associated antigen 5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGCCAC TGAAAGCACA AATTTCTGAT GAAAACAGGA TTACGACAAT GCAGACTGCA 
    CAACGCAAAT CTGTTGGGAG GACTCCGCTC AAAGATTTGA CAGCTCAGTC TTTTACATTG
    TCCGAGCAAA ATAGTTTGGT TAAAACAAGG TTAAATTCAA ACAAATCCCC AAGCCTCACA
    GTGAACAAAC AGCACTTCAA GGAAAATAAG AGTGGCAACA CTGGAACATC ACCAGTTAGC
    ATATGGGTGG AATCTGAGAC ACAAGTTCCT CTTATGGACA ACACCATAAG TGAAGTGTGT
    GCTTCAAAGC GTTTAAAACC AATTACCCTT TTTGAGACAA AGTTTACTAC ATTGAACTCA
    GATGGATTAG CTCCCAAAAT GAAAAGTAAA GATTCTAGCA ATATACATGA GTGTTTGCAG
    CCTTCAGAAC CTGCAACTTC TGAAAAGATA CTGTGCTTAT CTGCCTGTGG TCAAGAAAAG
    TTTCCCCTCA ATTGCTTGGA CAGTGACTCT TCTGTTCCCA GTAATGCAGA TCCTTGCATG
    AATGTGACAG CAAGCCTGCC TGTGGAAACA CAAATTAATG AACAGGAGTC TAGAAAAGAC
    AGGGATCAAA AGTTAGAAGC AGACACATGC CCAGTGACTG TTCCACTCTC TCACAAATCA
    GTGGTTGTTT CTCAATGTTC TAGCCAGGCA TTGGGTGAAA CAAGGAAGAG TCTTGAATTA
    CAATTGTGTG CAAATGAAGC TCTAAAAGAA TCACCCTTTG GCATAAGCCT AACTATAAAT
    GAAGCATTTT CTCATGCTGC ATCCACATTC TGCATTCCCG AACAAAATGA TGTTGCTGTG
    TCACACCAAG AAATTATTGA TCCATCTTTA AGTAACCACT TTAATGAGAG TGCAAATGGA
    AATAACTGTC CCAACAAGAC TGAAGAAGTC CAGTTATTGT TGAGCCTAAA AGAGGAGGAG
    AGAGTCCCAA ATGCCTCAGA GCTTGAATCT TGCAAAATGT TAAATGGACT TGCTAATGAC
    ACATATTTAA TTGATACTTC ATCAGCAGCT TCACCTGAGC ATGTTGACAA ACACAGCTTT
    CCAGCATTGA CTACAAATTT AATTTCCACT GAGTATTCTG AACAGGATGG CCTTGACTGC
    TCTCAAATCA TATCAACTTT AGATAATACA CAACCTGAAG GCTATTATAA TAAGGCGGAT
    AAGACTGACA TCAACAAACC ACTGAAAGGT GATGACAGTG TTCTGCCTGT GTCCCATAAA
    ACTAAAGAAA GTCCTGCACC TTATCCTACA ACATATATCC CATTACAGCA ACTAGCTGAT
    AGTTCTTCTA AAATATTTCT TCATTCCTTC ATTCAAGAGG AAGTCTGTAA TAAGACTTTT
    GATCTAGACT GCATTACAAT AAAGGATAAG AAAGTAGACC TTAAATGTAT TGAACCAAAC
    AGTATTATAC ATACTAGGAG TTGTGATGAG ACTGATGATC TATTGGTAGA GTGTAAACTG
    CTACCAGAGC AGACTGGTGG CACCTTAAAA ATAAAAGATA TTGCAGATCG TGGCACTGAT
    TACAAGACAG AGTTAGAATC CTCCATTGTT TTAGAGGAAG AAATGGCTTC TAAGAACTAT
    GATGGTTCTC TTAAAAATGA AAATGTCAAA GTACATCCTT CCATTGTTGT GCAAAGTGAT
    ACTGGGCAAG CAGCTGTGCC GCTTGTTGCT TCTGCCAAGG CTCATGAAAG CAGGTCTGAT
    TCAGTAGCAA ACCCAAGGGA CTCTGTTGTA AATAACCAGC TACAAGCAAG GAATTTTGAT
    ATTCCAACAT TATACAAAAG TGTACTCAGC AAGATGTCTC CTAAAGGGGA AAGCTCTAAA
    TTGGAAGTTG GAACGTGTTC GACCCCAACT TCAACCTCAA ATACATCCAC ATGGACAACA
    CCTATAATGT TGCTGAACAA GAGCTTGAAT ACATCATGGG ACTTTGAAAA GGGGAGGTCA
    AGTCCAAGAG ACAATGCATC GGAGACAGAT TCCTTGCTCT GGAACTTCTC CAGGGAGTCC
    TTTAATAAAG CATCACGTGA GGAGTTGATG GATCGTTTAG AGAGTACTTT GATAGTTGTT
    GAAGTTCTTT CACGACAGCT TCAAGGCTGG CAGCAGAATC GAATTGGTTC TAGACCATCT
    GAACAGAGAG ACTCTGCCAC ACAAACATGT GTAACATACA CAAGTACGGA AGAGCAATAT
    TATCACAACT TGTATGTAGA AACCATGGAA AGACTTCAAA ACATGCAGCG ATGCAATGAC
    GTAGAAAAGA AACTGCAGGA GATACTTAAA GGGAGTGTTG ATGTGCTGAT ATCTAACAAG
    ACTCAATCTA TGTCATTGAT TGAGTTTGCT GAGAACATGC AAGACAAAAC TCAGGAGGAT
    AAAGCAGATA TTAGCAGGAA GATGTGCCAA GCCAGGTCAC TTATAGCTGA TCACATGACT
    TTATTGAATA AAATGAACAA GAAGATGCAA GCAAACCAGC ACCAAATGGC CGAAATGAGA
    ACTAGTATGG AAAAGGCAGT TGGTGATAAA GAAGCGGCTG ATCAGTGTTT GAAAGATCTT
    GAGACCCATT CTTCTACAGT CATAGCTCAG CTGCAACGTG ACTTGGAATC TGAGCAATTG
    CTTTGTGATG CTGTGAAAAA AGCTTATACA CAACAGCTCT CCTTTAATGA AGAATTGGTT
    GACTTTGCTA AAAAGGCTCA GTCTGTCTGC TCAGAAATGG AAGAGGATAG AGTCCAGTTG
    CAAGTAAAGT GTTCTCAGGC CAGGGAGCTC ATGTCAAAAC ACTGGGTCCT GTTTGACATG
    ATGAAAAATA AAACAGATCA TGCTGTGCAT AAGTTTGAAT CCATGAAAAG TGAGCGGGAT
    GCGGCTTACT TGGAAAAAAA TGAAATGTGC AATCAGTTAG AGGATGTGAA CTCACAGAAG
    GCTCAGCTGG AAACAGGAAT AGCTCACCTT AGTTCAGAGC TGGAGTCCCT GATGGAACAG
    ATATGTAAAC TTAAGTCTGA AAATGATCAC CTGAAACTGG AAAACTCTGA GCAGACGGAG
    GAACTGTGTG CTAAAGATTC GTCTATGAAA CTGCTAGAAA AAGAGCTTAA TGAGACAACC
    ATTCGGGAAC AAGAAATTAA AGAACACAAC AAGAAATTGT CAGCTGAAAC TGTGCCGAGG
    TTGGAGCAGA AGCTGTTAGA GGCATTACAA GAGAAAGAGG TTCTACAAAT AAAGATACAA
    GAGTTCCAAA AGCAGCACGC ATCCCAGACT GCATCTTATA AGGAGAGCAT TGAGTTCCTT
    GAGCAAGAGA ACCATGTGTG GTTTGAGCAG GTCGCAGAAA CGGAGTCACA GCTAAAGAAT
    AATCTCTTTG CCTTACGTGA GAGAAACCTG CAGTGTGAGA CCCAAAAGGA CAGAATAAGC
    GAACTACAGA GTGAACTGTT AAAACTGAAG GAAGAATTTC AGATGACAAA GGAGGCAGCA
    AACGATACGC TGCTGAAAAT GGGTAAAGAG ATATCGGATT CATCCACAGA AGCCGGCAAA
    ATCAGGGACA ACCTGCTGTG TGTGAAAGAA CAAATAATGG ACACATTACA TGCAGAGGTA
    GCAGGTGTTT CCCAAATGCT AGAGACTCCT GCCAGGGGAC TTGGGAGTTG CCCCTCTACT
    TACATGATCA ATAGCACCCT AAAGTCTCAA ACTAAGCAAT CTAAAACAGC CAAGAATGCC
    GAAAGCATTT GGAGTGAGAC AAGTGCCTTT ACCATTGTTC AACCAGTGGC CTCGCCCACT
    ACAGATAACA AGGAGGAAAC ACTTCCAGAT GTAATATGTG AATTAGGTGA AATTGTGTCA
    AACTTTGCTG TCTCCTCTTC TTATGTCATT GGAGTGAAGG AACAAGAAAT CGAAGACTTT
    AAGAGAGAAA TATCGGACCT AAAGGATGAG CTACAGAATA TGCACTTCAA AAACACAATT
    GAAAAACGTG ATTTAATGGA AGAGATTGGA ATTCTGAAAA GAAAAAATAG GAATCTGGAG
    GACTGTGTTA ACAGTAAGCA GCAGTGTGTT CAAGAGCTTG AGGAAATTGT AAATCAACAG
    GAACAGAGGT TACTACAGCA AGTTAGCAAG GAGAAAGAAC GAGAGGACAT GATGCAAGAA
    AATGCAACAT TAAAGCGATC CCTACAGCAG TGTGAAAGTG AATTACTAGT CCTTAAAGAT
    GAGCTGTCAA AGAACCCAAC TGAAGCAGCT AGAGACTGGA TGCAGGAGAA GTTACTTTTA
    CATAAGGATT TGCGAAAGCT AAGACTGATG CTTGTGGAAA CTGAAAATTC TAAGTCTGAA
    ATTCTACATC GAGCAATGAG ACATAGGAAT ATCTTGGAAG GAAATCTCGC CCGCTCTGAG
    CTGGAGTTAA AGAAGCTGGA TGATATTATT GAGAAAATAA GAAAGACTCT CTTGTCTATA
    CCTGAAGTTG TCTCCAACTG TGAAGAACTG AAACAAATGT TGGAATATCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812908.1
    CDS124..4620
    Translation

    Target ORF information:

    RefSeq Version XM_012957454.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm associated antigen 5 (spag5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957454.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTGGCCAC TGAAAGCACA AATTTCTGAT GAAAACAGGA TTACGACAAT GCAGACTGCA 
    CAACGCAAAT CTGTTGGGAG GACTCCGCTC AAAGATTTGA CAGCTCAGTC TTTTACATTG
    TCCGAGCAAA ATAGTTTGGT TAAAACAAGG TTAAATTCAA ACAAATCCCC AAGCCTCACA
    GTGAACAAAC AGCACTTCAA GGAAAATAAG AGTGGCAACA CTGGAACATC ACCAGTTAGC
    ATATGGGTGG AATCTGAGAC ACAAGTTCCT CTTATGGACA ACACCATAAG TGAAGTGTGT
    GCTTCAAAGC GTTTAAAACC AATTACCCTT TTTGAGACAA AGTTTACTAC ATTGAACTCA
    GATGGATTAG CTCCCAAAAT GAAAAGTAAA GATTCTAGCA ATATACATGA GTGTTTGCAG
    CCTTCAGAAC CTGCAACTTC TGAAAAGATA CTGTGCTTAT CTGCCTGTGG TCAAGAAAAG
    TTTCCCCTCA ATTGCTTGGA CAGTGACTCT TCTGTTCCCA GTAATGCAGA TCCTTGCATG
    AATGTGACAG CAAGCCTGCC TGTGGAAACA CAAATTAATG AACAGGAGTC TAGAAAAGAC
    AGGGATCAAA AGTTAGAAGC AGACACATGC CCAGTGACTG TTCCACTCTC TCACAAATCA
    GTGGTTGTTT CTCAATGTTC TAGCCAGGCA TTGGGTGAAA CAAGGAAGAG TCTTGAATTA
    CAATTGTGTG CAAATGAAGC TCTAAAAGAA TCACCCTTTG GCATAAGCCT AACTATAAAT
    GAAGCATTTT CTCATGCTGC ATCCACATTC TGCATTCCCG AACAAAATGA TGTTGCTGTG
    TCACACCAAG AAATTATTGA TCCATCTTTA AGTAACCACT TTAATGAGAG TGCAAATGGA
    AATAACTGTC CCAACAAGAC TGAAGAAGTC CAGTTATTGT TGAGCCTAAA AGAGGAGGAG
    AGAGTCCCAA ATGCCTCAGA GCTTGAATCT TGCAAAATGT TAAATGGACT TGCTAATGAC
    ACATATTTAA TTGATACTTC ATCAGCAGCT TCACCTGAGC ATGTTGACAA ACACAGCTTT
    CCAGCATTGA CTACAAATTT AATTTCCACT GAGTATTCTG AACAGGATGG CCTTGACTGC
    TCTCAAATCA TATCAACTTT AGATAATACA CAACCTGAAG GCTATTATAA TAAGGCGGAT
    AAGACTGACA TCAACAAACC ACTGAAAGGT GATGACAGTG TTCTGCCTGT GTCCCATAAA
    ACTAAAGAAA GTCCTGCACC TTATCCTACA ACATATATCC CATTACAGCA ACTAGCTGAT
    AGTTCTTCTA AAATATTTCT TCATTCCTTC ATTCAAGAGG AAGTCTGTAA TAAGACTTTT
    GATCTAGACT GCATTACAAT AAAGGATAAG AAAGTAGACC TTAAATGTAT TGAACCAAAC
    AGTATTATAC ATACTAGGAG TTGTGATGAG ACTGATGATC TATTGGTAGA GTGTAAACTG
    CTACCAGAGC AGACTGGTGG CACCTTAAAA ATAAAAGATA TTGCAGATCG TGGCACTGAT
    TACAAGACAG AGTTAGAATC CTCCATTGTT TTAGAGGAAG AAATGGCTTC TAAGAACTAT
    GATGGTTCTC TTAAAAATGA AAATGTCAAA GTACATCCTT CCATTGTTGT GCAAAGTGAT
    ACTGGGCAAG CAGCTGTGCC GCTTGTTGCT TCTGCCAAGG CTCATGAAAG CAGGTCTGAT
    TCAGTAGCAA ACCCAAGGGA CTCTGTTGTA AATAACCAGC TACAAGCAAG GAATTTTGAT
    ATTCCAACAT TATACAAAAG TGTACTCAGC AAGATGTCTC CTAAAGGGGA AAGCTCTAAA
    TTGGAAGTTG GAACGTGTTC GACCCCAACT TCAACCTCAA ATACATCCAC ATGGACAACA
    CCTATAATGT TGCTGAACAA GAGCTTGAAT ACATCATGGG ACTTTGAAAA GGGGAGGTCA
    AGTCCAAGAG ACAATGCATC GGAGACAGAT TCCTTGCTCT GGAACTTCTC CAGGGAGTCC
    TTTAATAAAG CATCACGTGA GGAGTTGATG GATCGTTTAG AGAGTACTTT GATAGTTGTT
    GAAGTTCTTT CACGACAGCT TCAAGGCTGG CAGCAGAATC GAATTGGTTC TAGACCATCT
    GAACAGAGAG ACTCTGCCAC ACAAACATGT GTAACATACA CAAGTACGGA AGAGCAATAT
    TATCACAACT TGTATGTAGA AACCATGGAA AGACTTCAAA ACATGCAGCG ATGCAATGAC
    GTAGAAAAGA AACTGCAGGA GATACTTAAA GGGAGTGTTG ATGTGCTGAT ATCTAACAAG
    ACTCAATCTA TGTCATTGAT TGAGTTTGCT GAGAACATGC AAGACAAAAC TCAGGAGGAT
    AAAGCAGATA TTAGCAGGAA GATGTGCCAA GCCAGGTCAC TTATAGCTGA TCACATGACT
    TTATTGAATA AAATGAACAA GAAGATGCAA GCAAACCAGC ACCAAATGGC CGAAATGAGA
    ACTAGTATGG AAAAGGCAGT TGGTGATAAA GAAGCGGCTG ATCAGTGTTT GAAAGATCTT
    GAGACCCATT CTTCTACAGT CATAGCTCAG CTGCAACGTG ACTTGGAATC TGAGCAATTG
    CTTTGTGATG CTGTGAAAAA AGCTTATACA CAACAGCTCT CCTTTAATGA AGAATTGGTT
    GACTTTGCTA AAAAGGCTCA GTCTGTCTGC TCAGAAATGG AAGAGGATAG AGTCCAGTTG
    CAAGTAAAGT GTTCTCAGGC CAGGGAGCTC ATGTCAAAAC ACTGGGTCCT GTTTGACATG
    ATGAAAAATA AAACAGATCA TGCTGTGCAT AAGTTTGAAT CCATGAAAAG TGAGCGGGAT
    GCGGCTTACT TGGAAAAAAA TGAAATGTGC AATCAGTTAG AGGATGTGAA CTCACAGAAG
    GCTCAGCTGG AAACAGGAAT AGCTCACCTT AGTTCAGAGC TGGAGTCCCT GATGGAACAG
    ATATGTAAAC TTAAGTCTGA AAATGATCAC CTGAAACTGG AAAACTCTGA GCAGACGGAG
    GAACTGTGTG CTAAAGATTC GTCTATGAAA CTGCTAGAAA AAGAGCTTAA TGAGACAACC
    ATTCGGGAAC AAGAAATTAA AGAACACAAC AAGAAATTGT CAGCTGAAAC TGTGCCGAGG
    TTGGAGCAGA AGCTGTTAGA GGCATTACAA GAGAAAGAGG TTCTACAAAT AAAGATACAA
    GAGTTCCAAA AGCAGCACGC ATCCCAGACT GCATCTTATA AGGAGAGCAT TGAGTTCCTT
    GAGCAAGAGA ACCATGTGTG GTTTGAGCAG GTCGCAGAAA CGGAGTCACA GCTAAAGAAT
    AATCTCTTTG CCTTACGTGA GAGAAACCTG CAGTGTGAGA CCCAAAAGGA CAGAATAAGC
    GAACTACAGA GTGAACTGTT AAAACTGAAG GAAGAATTTC AGATGACAAA GGAGGCAGCA
    AACGATACGC TGCTGAAAAT GGGTAAAGAG ATATCGGATT CATCCACAGA AGCCGGCAAA
    ATCAGGGACA ACCTGCTGTG TGTGAAAGAA CAAATAATGG ACACATTACA TGCAGAGGTA
    GCAGGTGTTT CCCAAATGCT AGAGACTCCT GCCAGGGGAC TTGGGAGTTG CCCCTCTACT
    TACATGATCA ATAGCACCCT AAAGTCTCAA ACTAAGCAAT CTAAAACAGC CAAGAATGCC
    GAAAGCATTT GGAGTGAGAC AAGTGCCTTT ACCATTGTTC AACCAGTGGC CTCGCCCACT
    ACAGATAACA AGGAGGAAAC ACTTCCAGAT GTAATATGTG AATTAGGTGA AATTGTGTCA
    AACTTTGCTG TCTCCTCTTC TTATGTCATT GGAGTGAAGG AACAAGAAAT CGAAGACTTT
    AAGAGAGAAA TATCGGACCT AAAGGATGAG CTACAGAATA TGCACTTCAA AAACACAATT
    GAAAAACGTG ATTTAATGGA AGAGATTGGA ATTCTGAAAA GAAAAAATAG GAATCTGGAG
    GACTGTGTTA ACAGTAAGCA GCAGTGTGTT CAAGAGCTTG AGGAAATTGT AAATCAACAG
    GAACAGAGGT TACTACAGCA AGTTAGCAAG GAGAAAGAAC GAGAGGACAT GATGCAAGAA
    AATGCAACAT TAAAGCGATC CCTACAGCAG TGTGAAAGTG AATTACTAGT CCTTAAAGAT
    GAGCTGTCAA AGAACCCAAC TGAAGCAGCT AGAGACTGGA TGCAGGAGAA GTTACTTTTA
    CATAAGGATT TGCGAAAGCT AAGACTGATG CTTGTGGAAA CTGAAAATTC TAAGTCTGAA
    ATTCTACATC GAGCAATGAG ACATAGGAAT ATCTTGGAAG GAAATCTCGC CCGCTCTGAG
    CTGGAGTTAA AGAAGCTGGA TGATATTATT GAGAAAATAA GAAAGACTCT CTTGTCTATA
    CCTGAAGTTG TCTCCAACTG TGAAGAACTG AAACAAATGT TGGAATATCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.