myo1h cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo1h
Entrez Gene ID	100492763
Full Name	myosin IH
General protein information	Preferred Names myosin IH Names unconventional myosin-Ih
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018089962.1	XP_017945451.1	unconventional myosin-Ih
XM_018089962.2	XP_017945451.1	unconventional myosin-Ih

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYO1H	XP_002694646.2
Mus musculus (house mouse)	Myo1h	NP_001158045.1
Gallus gallus (chicken)	MYO1H	XP_003642232.2
Canis lupus familiaris (dog)	MYO1H	XP_005636383.1
Homo sapiens (human)	MYO1H	NP_001094891.3
Danio rerio (zebrafish)	myo1ha	XP_684539.5
Danio rerio (zebrafish)	myo1hb	XP_001337167.4
Xenopus tropicalis (tropical clawed frog)	myo1h	XP_002932109.2
Pan troglodytes (chimpanzee)	MYO1H	XP_509352.3

Gene Ontology

The following myo1h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo1h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42150	XM_018089962.2 Latest version!	Xenopus tropicalis myosin IH (myo1h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa42150	XM_018089962.1	Xenopus tropicalis myosin IH (myo1h), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo1h ( XM_018089962.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945451.1 Xenopus tropicalis myosin IH (myo1h), mRNA.

Price & Availability↑

CloneID	OXa42150
Clone ID Related Accession (Same CDS sequence)	XM_018089962.1 , XM_018089962.2
Accession Version	XM_018089962.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Ih
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC AGCAACAGGG AGGAAAAGAG AATGGGCGTA TGCACATGGA AGGGTCTTTG 
TCTGCAAGGG ACAAAGTTGG TGTACAGGAC TTTGTTTTGC TGGATTCCCA TACTAGTGAA 
GCAGCTTTCA TGGACAATCT ACGCAAACGA TTCCAGGAGA ACCTTATTTA TACATACATA 
GGAACAATGC TGGTGTCTGT GAATCCATAC CAAGAGCTTC AGCTGTACAC CCAGAGGCAG 
ATGGAGCTGT ACCAAGGGGT CAATTTTATT GAGCTTCCAC CACACATATA TGCAGTTGCT 
GATAATGCAT ACAGAGTAAT GTGCACAGAG CGTAATAACC ACTTTATCCT GATCTCTGGC 
GAAAGTGGTT CTGGGAAGAC AGAGACCTCT AAGAAGATTC TTCAGTACCT AGCCATGACA 
TGTCCAGTCA CTGAGAAACT TCAGACAATC AGAGATCGTC TCCTTCTCTC TAATCCTGTA 
CTAGAGGCTT TTGGAAATGC CAAAACAATC CGCAATGACA ATTCAAGCCG ATTTGGAAAA 
TACATGGATG TGCAGTTTGA CTACAAGGGA GCCCCAGCTG GAGGACATAT CCTGAGCTAC 
CTGATAGAAA AATCACGTGT GGTTCATCAG AATCCTGGGG AGAGGAACTT TCACATTTTC 
TATCAGCTGC TAGAAGGAGG CGAGGATCAT CTTATCCGTT GGTTGGGCCT GGAACGTAAT 
CCACAGATGT ACAGTTACCT GATCCAAGGT GAGTGTGCCA AAGTAAGCTC CATCTGTGAC 
AAAACCAACT GGAAGCTTGT CCGTAGGGCA TTCACTATTA TTGAATTCAC GGAAGCAGAT 
ATTGAGCATC TTTTTGGAAT AGTAGCCAGT GTTCTACATC TGGGGAATAT AAAGTATGAA 
GAAGACACCC ATGGTCATGC AATCATCAGG AATGGATCTC AGATGCAGTG GATATCTAAG 
CTACTTGGTG TTCCCGTGTC CATTTTGCAA GAAGCACTGA CAAATAAAAA GATTGAAGCC 
AGGTCAGAAG AGGTTCTCAG TCCTTTGAAT TTAGAGCTGG CTTACTATGC ACGTGATGCC 
CTCGCCAAAG CTATTTATGG AAGGTCTTTC AATTGGCTGG TCAACAAAAT TAATAGCTCT 
CTGGCCAACA AGGACCCACC AAAGAAAACA GTTATTGGAC TTCTTGACAT TTATGGATTT 
GAGGTTTTTG AGACAAACAG CTTTGAACAG TTCTGCATTA ATTACTGCAA TGAAAAGCTA 
CAGCAGCTCT TTATTTCCAT GACACTAAAG GCAGAACAAG AGGAATATGA AATAGAAGGT 
ATTGAGTGGG AACCTGTACA ATACTTTGAC AATAAAATTA TCTGTGATCT AATTGAAGAG 
AAGTACAAAG GGATCATTGC CATAATGGAC GAAGAGTGCC TGAGACCTGG GGAGGCCACA 
GACCTCAGCT TCTTAGCAAA ACTGGAAGAG AAGGTTGGGG ATCACCCCCA TTTCTTAACG 
CGCAAACTTG CAGATCCAGT AGCCAGAAAA ACTATAGACT GGGTGGATTT CAGGCTTATT 
CACTATGCAG GAGAAGTTAC ATATTGCGCA GTTGGATTTT TGGAGAAAAA TAATGATTTG 
CTGTACAGAA ATTTGAAAGA GGTGGTTCTG AAATCCAAAA ACTATATTTT AAGAGATTGT 
TTTCATCCCA CCGAGTTGGA CAATAGGAGG AGGCCGGAGA CTGTAACAAC ACAGTTCAAG 
AACAGCTTGT CATCTCTGAT AGAAATCCTT CTGTTAAAGG AGCCTACATA CATCAGGTGT 
ATCAAGCCGA ATAACACCAA ACAACCTGGA AAGTTTGAAG ATATGCTTGT CCGCCATCAG 
GTAAAGTACC TTGGCCTCAT GGAGCATTTA CGGGTCAGAA GAGCCGGATT TGCCTATCGA 
AGGAAATATG AGATATTCTT GGAAAGGTAC AAATCACTGT GTCCACAAAC TTGGCCTAAC 
TGGGCAGGTC CAGCCCGAGA AGGAGTAGAA AAACTAGTGA AGTACCTTGG ATACCAACCT 
AATGAATACA AATTAGGAAG AACAAAGCTT TTCATACGTT TTCCACGTAC GCTGTTTGCC 
ACTGTGGATT CATTTGAAAT AAGAAAGCAT CAGCTGGTTT CAAAAATCCA AGCCAAATAC 
AAAGGGTGTT TAGGAAAAAG AGCTTTTGAG AAGAAGAAAA AAGCAGCTAT TAAGCTAGAG 
TCATGCTGGA GAGGGGTATT AGCTCGCAGA GAAGCAAAGA AGCGTGCATG GGCTGTGCAG 
ACCATCAGAA AGTTCATTGT TGGATTTATC AATCGGAAAA ATCCAGATGC AGACACAAAC 
AAAAACTTCT TTGGCATGAT GTATTACAAC TATCTTTGGA AACTGAGGGA ACATGTGCCC 
AAAAACGTGT TGGATAAAAG ATGGCTGACC CCACCAGTAG CCATGGAAAA GACATCAGAA 
CTTCTACACA AGCTATGCAT AAGAAACCTA GTGAGGAAGT ACTGCAGGAC AATCACCCCT 
CAGAGAACAC ACCAGCTGCA GCAGAAAGTT GTGACCAGTC AAATCTTCAA AGGGAAGAAG 
GAGGGTTACA AGCAAAGTCT CTGCCAGCCT TTCGAAGAGA CTCGATTAAG CGAACAAGAT 
ATTAACCCTA AAGTGCTTCA ACTAATGAAT TATGACAGGA TTAAGTATGG AATAGCAGCG 
GTGAAATATG ACAGACATGG ATTCAAAGCT CGCCCCCGCC AACTCCTATT GACGCAGTCA 
GCAGCGTATG TGACCGAGCA AGCCAAGATA AAGCAGAAAA TCGAGTATTC TGGGCTGAAA 
GGAATCTCTG TCAGTAACCT GAATGATGGA TTTCTGATCA TCCATGTATC CCCAGAGGAC 
AACAAGCAGA AGGGAGATGC CATATTACAG TGTGACCACA TCTTTGAAGC AGTAACCAAA 
CTGTGCATGC TGGCTTCTAA GCAAGATGCA GTTACCATAG CGCAGGGAAG CTTAACCTTC 
CACTTCCAAG GCAAAGAAGG CATCATTGAT TTCACACATG GGGCTGAAGC ACAAGTGTAC 
AAAGCCAAGA ATGGGCACCT AACAGTGGCC ACACCAAGAC TGAAATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945451.1
CDS	248..3358
Translation	METQQQGGKENGRMHMEGSLSARDKVGVQDFVLLDSHTSEAAFMDNLRKRFQENLIYTYIGTMLVSVNPYQELQLYTQRQMELYQGVNFIELPPHIYAVADNAYRVMCTERNNHFILISGESGSGKTETSKKILQYLAMTCPVTEKLQTIRDRLLLSNPVLEAFGNAKTIRNDNSSRFGKYMDVQFDYKGAPAGGHILSYLIEKSRVVHQNPGERNFHIFYQLLEGGEDHLIRWLGLERNPQMYSYLIQGECAKVSSICDKTNWKLVRRAFTIIEFTEADIEHLFGIVASVLHLGNIKYEEDTHGHAIIRNGSQMQWISKLLGVPVSILQEALTNKKIEARSEEVLSPLNLELAYYARDALAKAIYGRSFNWLVNKINSSLANKDPPKKTVIGLLDIYGFEVFETNSFEQFCINYCNEKLQQLFISMTLKAEQEEYEIEGIEWEPVQYFDNKIICDLIEEKYKGIIAIMDEECLRPGEATDLSFLAKLEEKVGDHPHFLTRKLADPVARKTIDWVDFRLIHYAGEVTYCAVGFLEKNNDLLYRNLKEVVLKSKNYILRDCFHPTELDNRRRPETVTTQFKNSLSSLIEILLLKEPTYIRCIKPNNTKQPGKFEDMLVRHQVKYLGLMEHLRVRRAGFAYRRKYEIFLERYKSLCPQTWPNWAGPAREGVEKLVKYLGYQPNEYKLGRTKLFIRFPRTLFATVDSFEIRKHQLVSKIQAKYKGCLGKRAFEKKKKAAIKLESCWRGVLARREAKKRAWAVQTIRKFIVGFINRKNPDADTNKNFFGMMYYNYLWKLREHVPKNVLDKRWLTPPVAMEKTSELLHKLCIRNLVRKYCRTITPQRTHQLQQKVVTSQIFKGKKEGYKQSLCQPFEETRLSEQDINPKVLQLMNYDRIKYGIAAVKYDRHGFKARPRQLLLTQSAAYVTEQAKIKQKIEYSGLKGISVSNLNDGFLIIHVSPEDNKQKGDAILQCDHIFEAVTKLCMLASKQDAVTIAQGSLTFHFQGKEGIIDFTHGAEAQVYKAKNGHLTVATPRLKS

Target ORF information:

RefSeq Version	XM_018089962.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin IH (myo1h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089962.1

ORF Insert Sequence:

ATGGAAACAC AGCAACAGGG AGGAAAAGAG AATGGGCGTA TGCACATGGA AGGGTCTTTG 
TCTGCAAGGG ACAAAGTTGG TGTACAGGAC TTTGTTTTGC TGGATTCCCA TACTAGTGAA 
GCAGCTTTCA TGGACAATCT ACGCAAACGA TTCCAGGAGA ACCTTATTTA TACATACATA 
GGAACAATGC TGGTGTCTGT GAATCCATAC CAAGAGCTTC AGCTGTACAC CCAGAGGCAG 
ATGGAGCTGT ACCAAGGGGT CAATTTTATT GAGCTTCCAC CACACATATA TGCAGTTGCT 
GATAATGCAT ACAGAGTAAT GTGCACAGAG CGTAATAACC ACTTTATCCT GATCTCTGGC 
GAAAGTGGTT CTGGGAAGAC AGAGACCTCT AAGAAGATTC TTCAGTACCT AGCCATGACA 
TGTCCAGTCA CTGAGAAACT TCAGACAATC AGAGATCGTC TCCTTCTCTC TAATCCTGTA 
CTAGAGGCTT TTGGAAATGC CAAAACAATC CGCAATGACA ATTCAAGCCG ATTTGGAAAA 
TACATGGATG TGCAGTTTGA CTACAAGGGA GCCCCAGCTG GAGGACATAT CCTGAGCTAC 
CTGATAGAAA AATCACGTGT GGTTCATCAG AATCCTGGGG AGAGGAACTT TCACATTTTC 
TATCAGCTGC TAGAAGGAGG CGAGGATCAT CTTATCCGTT GGTTGGGCCT GGAACGTAAT 
CCACAGATGT ACAGTTACCT GATCCAAGGT GAGTGTGCCA AAGTAAGCTC CATCTGTGAC 
AAAACCAACT GGAAGCTTGT CCGTAGGGCA TTCACTATTA TTGAATTCAC GGAAGCAGAT 
ATTGAGCATC TTTTTGGAAT AGTAGCCAGT GTTCTACATC TGGGGAATAT AAAGTATGAA 
GAAGACACCC ATGGTCATGC AATCATCAGG AATGGATCTC AGATGCAGTG GATATCTAAG 
CTACTTGGTG TTCCCGTGTC CATTTTGCAA GAAGCACTGA CAAATAAAAA GATTGAAGCC 
AGGTCAGAAG AGGTTCTCAG TCCTTTGAAT TTAGAGCTGG CTTACTATGC ACGTGATGCC 
CTCGCCAAAG CTATTTATGG AAGGTCTTTC AATTGGCTGG TCAACAAAAT TAATAGCTCT 
CTGGCCAACA AGGACCCACC AAAGAAAACA GTTATTGGAC TTCTTGACAT TTATGGATTT 
GAGGTTTTTG AGACAAACAG CTTTGAACAG TTCTGCATTA ATTACTGCAA TGAAAAGCTA 
CAGCAGCTCT TTATTTCCAT GACACTAAAG GCAGAACAAG AGGAATATGA AATAGAAGGT 
ATTGAGTGGG AACCTGTACA ATACTTTGAC AATAAAATTA TCTGTGATCT AATTGAAGAG 
AAGTACAAAG GGATCATTGC CATAATGGAC GAAGAGTGCC TGAGACCTGG GGAGGCCACA 
GACCTCAGCT TCTTAGCAAA ACTGGAAGAG AAGGTTGGGG ATCACCCCCA TTTCTTAACG 
CGCAAACTTG CAGATCCAGT AGCCAGAAAA ACTATAGACT GGGTGGATTT CAGGCTTATT 
CACTATGCAG GAGAAGTTAC ATATTGCGCA GTTGGATTTT TGGAGAAAAA TAATGATTTG 
CTGTACAGAA ATTTGAAAGA GGTGGTTCTG AAATCCAAAA ACTATATTTT AAGAGATTGT 
TTTCATCCCA CCGAGTTGGA CAATAGGAGG AGGCCGGAGA CTGTAACAAC ACAGTTCAAG 
AACAGCTTGT CATCTCTGAT AGAAATCCTT CTGTTAAAGG AGCCTACATA CATCAGGTGT 
ATCAAGCCGA ATAACACCAA ACAACCTGGA AAGTTTGAAG ATATGCTTGT CCGCCATCAG 
GTAAAGTACC TTGGCCTCAT GGAGCATTTA CGGGTCAGAA GAGCCGGATT TGCCTATCGA 
AGGAAATATG AGATATTCTT GGAAAGGTAC AAATCACTGT GTCCACAAAC TTGGCCTAAC 
TGGGCAGGTC CAGCCCGAGA AGGAGTAGAA AAACTAGTGA AGTACCTTGG ATACCAACCT 
AATGAATACA AATTAGGAAG AACAAAGCTT TTCATACGTT TTCCACGTAC GCTGTTTGCC 
ACTGTGGATT CATTTGAAAT AAGAAAGCAT CAGCTGGTTT CAAAAATCCA AGCCAAATAC 
AAAGGGTGTT TAGGAAAAAG AGCTTTTGAG AAGAAGAAAA AAGCAGCTAT TAAGCTAGAG 
TCATGCTGGA GAGGGGTATT AGCTCGCAGA GAAGCAAAGA AGCGTGCATG GGCTGTGCAG 
ACCATCAGAA AGTTCATTGT TGGATTTATC AATCGGAAAA ATCCAGATGC AGACACAAAC 
AAAAACTTCT TTGGCATGAT GTATTACAAC TATCTTTGGA AACTGAGGGA ACATGTGCCC 
AAAAACGTGT TGGATAAAAG ATGGCTGACC CCACCAGTAG CCATGGAAAA GACATCAGAA 
CTTCTACACA AGCTATGCAT AAGAAACCTA GTGAGGAAGT ACTGCAGGAC AATCACCCCT 
CAGAGAACAC ACCAGCTGCA GCAGAAAGTT GTGACCAGTC AAATCTTCAA AGGGAAGAAG 
GAGGGTTACA AGCAAAGTCT CTGCCAGCCT TTCGAAGAGA CTCGATTAAG CGAACAAGAT 
ATTAACCCTA AAGTGCTTCA ACTAATGAAT TATGACAGGA TTAAGTATGG AATAGCAGCG 
GTGAAATATG ACAGACATGG ATTCAAAGCT CGCCCCCGCC AACTCCTATT GACGCAGTCA 
GCAGCGTATG TGACCGAGCA AGCCAAGATA AAGCAGAAAA TCGAGTATTC TGGGCTGAAA 
GGAATCTCTG TCAGTAACCT GAATGATGGA TTTCTGATCA TCCATGTATC CCCAGAGGAC 
AACAAGCAGA AGGGAGATGC CATATTACAG TGTGACCACA TCTTTGAAGC AGTAACCAAA 
CTGTGCATGC TGGCTTCTAA GCAAGATGCA GTTACCATAG CGCAGGGAAG CTTAACCTTC 
CACTTCCAAG GCAAAGAAGG CATCATTGAT TTCACACATG GGGCTGAAGC ACAAGTGTAC 
AAAGCCAAGA ATGGGCACCT AACAGTGGCC ACACCAAGAC TGAAATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo1h ( XM_018089962.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945451.1 Xenopus tropicalis myosin IH (myo1h), mRNA.

Price & Availability↑

CloneID	OXa42150
Clone ID Related Accession (Same CDS sequence)	XM_018089962.1 , XM_018089962.2
Accession Version	XM_018089962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Ih
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC AGCAACAGGG AGGAAAAGAG AATGGGCGTA TGCACATGGA AGGGTCTTTG 
TCTGCAAGGG ACAAAGTTGG TGTACAGGAC TTTGTTTTGC TGGATTCCCA TACTAGTGAA 
GCAGCTTTCA TGGACAATCT ACGCAAACGA TTCCAGGAGA ACCTTATTTA TACATACATA 
GGAACAATGC TGGTGTCTGT GAATCCATAC CAAGAGCTTC AGCTGTACAC CCAGAGGCAG 
ATGGAGCTGT ACCAAGGGGT CAATTTTATT GAGCTTCCAC CACACATATA TGCAGTTGCT 
GATAATGCAT ACAGAGTAAT GTGCACAGAG CGTAATAACC ACTTTATCCT GATCTCTGGC 
GAAAGTGGTT CTGGGAAGAC AGAGACCTCT AAGAAGATTC TTCAGTACCT AGCCATGACA 
TGTCCAGTCA CTGAGAAACT TCAGACAATC AGAGATCGTC TCCTTCTCTC TAATCCTGTA 
CTAGAGGCTT TTGGAAATGC CAAAACAATC CGCAATGACA ATTCAAGCCG ATTTGGAAAA 
TACATGGATG TGCAGTTTGA CTACAAGGGA GCCCCAGCTG GAGGACATAT CCTGAGCTAC 
CTGATAGAAA AATCACGTGT GGTTCATCAG AATCCTGGGG AGAGGAACTT TCACATTTTC 
TATCAGCTGC TAGAAGGAGG CGAGGATCAT CTTATCCGTT GGTTGGGCCT GGAACGTAAT 
CCACAGATGT ACAGTTACCT GATCCAAGGT GAGTGTGCCA AAGTAAGCTC CATCTGTGAC 
AAAACCAACT GGAAGCTTGT CCGTAGGGCA TTCACTATTA TTGAATTCAC GGAAGCAGAT 
ATTGAGCATC TTTTTGGAAT AGTAGCCAGT GTTCTACATC TGGGGAATAT AAAGTATGAA 
GAAGACACCC ATGGTCATGC AATCATCAGG AATGGATCTC AGATGCAGTG GATATCTAAG 
CTACTTGGTG TTCCCGTGTC CATTTTGCAA GAAGCACTGA CAAATAAAAA GATTGAAGCC 
AGGTCAGAAG AGGTTCTCAG TCCTTTGAAT TTAGAGCTGG CTTACTATGC ACGTGATGCC 
CTCGCCAAAG CTATTTATGG AAGGTCTTTC AATTGGCTGG TCAACAAAAT TAATAGCTCT 
CTGGCCAACA AGGACCCACC AAAGAAAACA GTTATTGGAC TTCTTGACAT TTATGGATTT 
GAGGTTTTTG AGACAAACAG CTTTGAACAG TTCTGCATTA ATTACTGCAA TGAAAAGCTA 
CAGCAGCTCT TTATTTCCAT GACACTAAAG GCAGAACAAG AGGAATATGA AATAGAAGGT 
ATTGAGTGGG AACCTGTACA ATACTTTGAC AATAAAATTA TCTGTGATCT AATTGAAGAG 
AAGTACAAAG GGATCATTGC CATAATGGAC GAAGAGTGCC TGAGACCTGG GGAGGCCACA 
GACCTCAGCT TCTTAGCAAA ACTGGAAGAG AAGGTTGGGG ATCACCCCCA TTTCTTAACG 
CGCAAACTTG CAGATCCAGT AGCCAGAAAA ACTATAGACT GGGTGGATTT CAGGCTTATT 
CACTATGCAG GAGAAGTTAC ATATTGCGCA GTTGGATTTT TGGAGAAAAA TAATGATTTG 
CTGTACAGAA ATTTGAAAGA GGTGGTTCTG AAATCCAAAA ACTATATTTT AAGAGATTGT 
TTTCATCCCA CCGAGTTGGA CAATAGGAGG AGGCCGGAGA CTGTAACAAC ACAGTTCAAG 
AACAGCTTGT CATCTCTGAT AGAAATCCTT CTGTTAAAGG AGCCTACATA CATCAGGTGT 
ATCAAGCCGA ATAACACCAA ACAACCTGGA AAGTTTGAAG ATATGCTTGT CCGCCATCAG 
GTAAAGTACC TTGGCCTCAT GGAGCATTTA CGGGTCAGAA GAGCCGGATT TGCCTATCGA 
AGGAAATATG AGATATTCTT GGAAAGGTAC AAATCACTGT GTCCACAAAC TTGGCCTAAC 
TGGGCAGGTC CAGCCCGAGA AGGAGTAGAA AAACTAGTGA AGTACCTTGG ATACCAACCT 
AATGAATACA AATTAGGAAG AACAAAGCTT TTCATACGTT TTCCACGTAC GCTGTTTGCC 
ACTGTGGATT CATTTGAAAT AAGAAAGCAT CAGCTGGTTT CAAAAATCCA AGCCAAATAC 
AAAGGGTGTT TAGGAAAAAG AGCTTTTGAG AAGAAGAAAA AAGCAGCTAT TAAGCTAGAG 
TCATGCTGGA GAGGGGTATT AGCTCGCAGA GAAGCAAAGA AGCGTGCATG GGCTGTGCAG 
ACCATCAGAA AGTTCATTGT TGGATTTATC AATCGGAAAA ATCCAGATGC AGACACAAAC 
AAAAACTTCT TTGGCATGAT GTATTACAAC TATCTTTGGA AACTGAGGGA ACATGTGCCC 
AAAAACGTGT TGGATAAAAG ATGGCTGACC CCACCAGTAG CCATGGAAAA GACATCAGAA 
CTTCTACACA AGCTATGCAT AAGAAACCTA GTGAGGAAGT ACTGCAGGAC AATCACCCCT 
CAGAGAACAC ACCAGCTGCA GCAGAAAGTT GTGACCAGTC AAATCTTCAA AGGGAAGAAG 
GAGGGTTACA AGCAAAGTCT CTGCCAGCCT TTCGAAGAGA CTCGATTAAG CGAACAAGAT 
ATTAACCCTA AAGTGCTTCA ACTAATGAAT TATGACAGGA TTAAGTATGG AATAGCAGCG 
GTGAAATATG ACAGACATGG ATTCAAAGCT CGCCCCCGCC AACTCCTATT GACGCAGTCA 
GCAGCGTATG TGACCGAGCA AGCCAAGATA AAGCAGAAAA TCGAGTATTC TGGGCTGAAA 
GGAATCTCTG TCAGTAACCT GAATGATGGA TTTCTGATCA TCCATGTATC CCCAGAGGAC 
AACAAGCAGA AGGGAGATGC CATATTACAG TGTGACCACA TCTTTGAAGC AGTAACCAAA 
CTGTGCATGC TGGCTTCTAA GCAAGATGCA GTTACCATAG CGCAGGGAAG CTTAACCTTC 
CACTTCCAAG GCAAAGAAGG CATCATTGAT TTCACACATG GGGCTGAAGC ACAAGTGTAC 
AAAGCCAAGA ATGGGCACCT AACAGTGGCC ACACCAAGAC TGAAATCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945451.1
CDS	132..3242
Translation	METQQQGGKENGRMHMEGSLSARDKVGVQDFVLLDSHTSEAAFMDNLRKRFQENLIYTYIGTMLVSVNPYQELQLYTQRQMELYQGVNFIELPPHIYAVADNAYRVMCTERNNHFILISGESGSGKTETSKKILQYLAMTCPVTEKLQTIRDRLLLSNPVLEAFGNAKTIRNDNSSRFGKYMDVQFDYKGAPAGGHILSYLIEKSRVVHQNPGERNFHIFYQLLEGGEDHLIRWLGLERNPQMYSYLIQGECAKVSSICDKTNWKLVRRAFTIIEFTEADIEHLFGIVASVLHLGNIKYEEDTHGHAIIRNGSQMQWISKLLGVPVSILQEALTNKKIEARSEEVLSPLNLELAYYARDALAKAIYGRSFNWLVNKINSSLANKDPPKKTVIGLLDIYGFEVFETNSFEQFCINYCNEKLQQLFISMTLKAEQEEYEIEGIEWEPVQYFDNKIICDLIEEKYKGIIAIMDEECLRPGEATDLSFLAKLEEKVGDHPHFLTRKLADPVARKTIDWVDFRLIHYAGEVTYCAVGFLEKNNDLLYRNLKEVVLKSKNYILRDCFHPTELDNRRRPETVTTQFKNSLSSLIEILLLKEPTYIRCIKPNNTKQPGKFEDMLVRHQVKYLGLMEHLRVRRAGFAYRRKYEIFLERYKSLCPQTWPNWAGPAREGVEKLVKYLGYQPNEYKLGRTKLFIRFPRTLFATVDSFEIRKHQLVSKIQAKYKGCLGKRAFEKKKKAAIKLESCWRGVLARREAKKRAWAVQTIRKFIVGFINRKNPDADTNKNFFGMMYYNYLWKLREHVPKNVLDKRWLTPPVAMEKTSELLHKLCIRNLVRKYCRTITPQRTHQLQQKVVTSQIFKGKKEGYKQSLCQPFEETRLSEQDINPKVLQLMNYDRIKYGIAAVKYDRHGFKARPRQLLLTQSAAYVTEQAKIKQKIEYSGLKGISVSNLNDGFLIIHVSPEDNKQKGDAILQCDHIFEAVTKLCMLASKQDAVTIAQGSLTFHFQGKEGIIDFTHGAEAQVYKAKNGHLTVATPRLKS

Target ORF information:

RefSeq Version	XM_018089962.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin IH (myo1h), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089962.2

ORF Insert Sequence:

ATGGAAACAC AGCAACAGGG AGGAAAAGAG AATGGGCGTA TGCACATGGA AGGGTCTTTG 
TCTGCAAGGG ACAAAGTTGG TGTACAGGAC TTTGTTTTGC TGGATTCCCA TACTAGTGAA 
GCAGCTTTCA TGGACAATCT ACGCAAACGA TTCCAGGAGA ACCTTATTTA TACATACATA 
GGAACAATGC TGGTGTCTGT GAATCCATAC CAAGAGCTTC AGCTGTACAC CCAGAGGCAG 
ATGGAGCTGT ACCAAGGGGT CAATTTTATT GAGCTTCCAC CACACATATA TGCAGTTGCT 
GATAATGCAT ACAGAGTAAT GTGCACAGAG CGTAATAACC ACTTTATCCT GATCTCTGGC 
GAAAGTGGTT CTGGGAAGAC AGAGACCTCT AAGAAGATTC TTCAGTACCT AGCCATGACA 
TGTCCAGTCA CTGAGAAACT TCAGACAATC AGAGATCGTC TCCTTCTCTC TAATCCTGTA 
CTAGAGGCTT TTGGAAATGC CAAAACAATC CGCAATGACA ATTCAAGCCG ATTTGGAAAA 
TACATGGATG TGCAGTTTGA CTACAAGGGA GCCCCAGCTG GAGGACATAT CCTGAGCTAC 
CTGATAGAAA AATCACGTGT GGTTCATCAG AATCCTGGGG AGAGGAACTT TCACATTTTC 
TATCAGCTGC TAGAAGGAGG CGAGGATCAT CTTATCCGTT GGTTGGGCCT GGAACGTAAT 
CCACAGATGT ACAGTTACCT GATCCAAGGT GAGTGTGCCA AAGTAAGCTC CATCTGTGAC 
AAAACCAACT GGAAGCTTGT CCGTAGGGCA TTCACTATTA TTGAATTCAC GGAAGCAGAT 
ATTGAGCATC TTTTTGGAAT AGTAGCCAGT GTTCTACATC TGGGGAATAT AAAGTATGAA 
GAAGACACCC ATGGTCATGC AATCATCAGG AATGGATCTC AGATGCAGTG GATATCTAAG 
CTACTTGGTG TTCCCGTGTC CATTTTGCAA GAAGCACTGA CAAATAAAAA GATTGAAGCC 
AGGTCAGAAG AGGTTCTCAG TCCTTTGAAT TTAGAGCTGG CTTACTATGC ACGTGATGCC 
CTCGCCAAAG CTATTTATGG AAGGTCTTTC AATTGGCTGG TCAACAAAAT TAATAGCTCT 
CTGGCCAACA AGGACCCACC AAAGAAAACA GTTATTGGAC TTCTTGACAT TTATGGATTT 
GAGGTTTTTG AGACAAACAG CTTTGAACAG TTCTGCATTA ATTACTGCAA TGAAAAGCTA 
CAGCAGCTCT TTATTTCCAT GACACTAAAG GCAGAACAAG AGGAATATGA AATAGAAGGT 
ATTGAGTGGG AACCTGTACA ATACTTTGAC AATAAAATTA TCTGTGATCT AATTGAAGAG 
AAGTACAAAG GGATCATTGC CATAATGGAC GAAGAGTGCC TGAGACCTGG GGAGGCCACA 
GACCTCAGCT TCTTAGCAAA ACTGGAAGAG AAGGTTGGGG ATCACCCCCA TTTCTTAACG 
CGCAAACTTG CAGATCCAGT AGCCAGAAAA ACTATAGACT GGGTGGATTT CAGGCTTATT 
CACTATGCAG GAGAAGTTAC ATATTGCGCA GTTGGATTTT TGGAGAAAAA TAATGATTTG 
CTGTACAGAA ATTTGAAAGA GGTGGTTCTG AAATCCAAAA ACTATATTTT AAGAGATTGT 
TTTCATCCCA CCGAGTTGGA CAATAGGAGG AGGCCGGAGA CTGTAACAAC ACAGTTCAAG 
AACAGCTTGT CATCTCTGAT AGAAATCCTT CTGTTAAAGG AGCCTACATA CATCAGGTGT 
ATCAAGCCGA ATAACACCAA ACAACCTGGA AAGTTTGAAG ATATGCTTGT CCGCCATCAG 
GTAAAGTACC TTGGCCTCAT GGAGCATTTA CGGGTCAGAA GAGCCGGATT TGCCTATCGA 
AGGAAATATG AGATATTCTT GGAAAGGTAC AAATCACTGT GTCCACAAAC TTGGCCTAAC 
TGGGCAGGTC CAGCCCGAGA AGGAGTAGAA AAACTAGTGA AGTACCTTGG ATACCAACCT 
AATGAATACA AATTAGGAAG AACAAAGCTT TTCATACGTT TTCCACGTAC GCTGTTTGCC 
ACTGTGGATT CATTTGAAAT AAGAAAGCAT CAGCTGGTTT CAAAAATCCA AGCCAAATAC 
AAAGGGTGTT TAGGAAAAAG AGCTTTTGAG AAGAAGAAAA AAGCAGCTAT TAAGCTAGAG 
TCATGCTGGA GAGGGGTATT AGCTCGCAGA GAAGCAAAGA AGCGTGCATG GGCTGTGCAG 
ACCATCAGAA AGTTCATTGT TGGATTTATC AATCGGAAAA ATCCAGATGC AGACACAAAC 
AAAAACTTCT TTGGCATGAT GTATTACAAC TATCTTTGGA AACTGAGGGA ACATGTGCCC 
AAAAACGTGT TGGATAAAAG ATGGCTGACC CCACCAGTAG CCATGGAAAA GACATCAGAA 
CTTCTACACA AGCTATGCAT AAGAAACCTA GTGAGGAAGT ACTGCAGGAC AATCACCCCT 
CAGAGAACAC ACCAGCTGCA GCAGAAAGTT GTGACCAGTC AAATCTTCAA AGGGAAGAAG 
GAGGGTTACA AGCAAAGTCT CTGCCAGCCT TTCGAAGAGA CTCGATTAAG CGAACAAGAT 
ATTAACCCTA AAGTGCTTCA ACTAATGAAT TATGACAGGA TTAAGTATGG AATAGCAGCG 
GTGAAATATG ACAGACATGG ATTCAAAGCT CGCCCCCGCC AACTCCTATT GACGCAGTCA 
GCAGCGTATG TGACCGAGCA AGCCAAGATA AAGCAGAAAA TCGAGTATTC TGGGCTGAAA 
GGAATCTCTG TCAGTAACCT GAATGATGGA TTTCTGATCA TCCATGTATC CCCAGAGGAC 
AACAAGCAGA AGGGAGATGC CATATTACAG TGTGACCACA TCTTTGAAGC AGTAACCAAA 
CTGTGCATGC TGGCTTCTAA GCAAGATGCA GTTACCATAG CGCAGGGAAG CTTAACCTTC 
CACTTCCAAG GCAAAGAAGG CATCATTGAT TTCACACATG GGGCTGAAGC ACAAGTGTAC 
AAAGCCAAGA ATGGGCACCT AACAGTGGCC ACACCAAGAC TGAAATCCTA A