LOC100490952 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100490952
Entrez Gene ID	100490952
Full Name	zinc finger protein 585A-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967563.3	XP_012823017.1	zinc finger protein 585A
XM_004916388.4	XP_004916445.1	zinc finger protein 585A
XM_004916388.3	XP_004916445.1	zinc finger protein 585A-like
XM_012967563.2	XP_012823017.1	zinc finger protein 585A-like

Pathways from BioSystems
Gene Ontology

The following LOC100490952 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100490952 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa13543	XM_012967563.3 Latest version!	Xenopus tropicalis zinc finger protein 585A (LOC100490952), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa13543	XM_004916388.4 Latest version!	Xenopus tropicalis zinc finger protein 585A (LOC100490952), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa13543	XM_004916388.3	Xenopus tropicalis zinc finger protein 585A-like (LOC100490952), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa13543	XM_012967563.2	Xenopus tropicalis zinc finger protein 585A-like (LOC100490952), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100490952 ( XM_012967563.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823017.1 Xenopus tropicalis zinc finger protein 585A (LOC100490952), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa13543
Clone ID Related Accession (Same CDS sequence)	XM_012967563.3 , XM_004916388.4 , XM_004916388.3 , XM_012967563.2
Accession Version	XM_012967563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 585A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823017.1
CDS	447..3917
Translation	MMENGEESYVMENDLSRPSHMELSECWVSERPEGIGSFGPVSGKMAEDQAARNSLDRSNANRQQNQWRDKAEHHQQEEEEVACQSGTVAADFSFLPGQSIAVPVPNVEKQRSREENIVAQRASEPQIVKIAEDARAQGPSFGKMEKDNMLICRPQAYNVGQQAKNIDVPTRKDIGSPEQSNQRYWGLFQCSFCNFVFQDLSELVQHQETHNQDKFQGIGQELVQLDQTESTQCEWRRYRCIVCDKTFCKQSSLVTHLRIHTGEKPYSCHVCRRKFNQRTSLTVHLRTHTGEAPFRCNKCNKSFRQQSNLTHHMKSHQRAEELDGGDWSEENRAPGFPLMYLVSEGDEFSNEVWGPKVSLIKGDKEEDSASCKRPFVCGHCFKRFTHQSNLMVHQRIHTGDRSYRCQECGKHFTRRTSLMVHLRGHTGEMPYSCQQCGKSFRQQSNLLYHMKSHAGQNDAAAGSTNKHTVKLSGQLIGPSGQYVERESPRPVSIGNSGVTRVPENQMRVYQCNQCSRWFPSTSSLLLHQKFHVEQPSNVMQSREVQVQYPIGISQPHTFTGNVLISQQEHERSQQQHFRLFSRMDGNNVPGMLNSSAPKVTPQSGSNSGYHKPGSTVEGGPIKGSFQIRGRGRPRKNIWFGSGQTAMGGFGTGKPLHKCWKCPRRFNNKSNLIVHLRIHTGEKPYQCWKCDKRFRQQSNLIQHLRNHEEFRDDDDDVPRQKKGMNKLRQSGKNFHNTAMLSSSGASQTHWSFLPFTDGNLMNQNRQLLEVSPVSQLEDAASHSDCSNDTLGNESAERSYKCGSCFKTFNHKSNLLVHERIHSGDKAYRCQECGKQFSQRTSLMVHLRTHTGEMPYSCQQCRRRFRQQSNLLYHIKTNTVHGQLKCTAENFINSVSRIDSGEDNRICTELDSIPGQAQAWMHLEPSSKTETAPALPQEAIKEEFPCPDCDKVFTHQSNLLVHQRIHSGERAYRCHECNRQFSQRTSLMIHLRTHTGEMPYACQCCGKRFRQQSNLLYHLKSHANQMAGMDVITSAEYTAPKTRGRPRKCDSNDERRAKNIIPRGKRTFKCMECPRRYNLMSNLVAHQRSHDRQPLHTCSDCGEGFLQQSYLTVHKKLHTKGDLPEHVGQFCWKQNQELMIRNPSIEEERGVLYTQHEK

Target ORF information:

RefSeq Version	XM_012967563.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 585A (LOC100490952), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967563.3

ORF Insert Sequence:

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100490952 ( XM_004916388.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916445.1 Xenopus tropicalis zinc finger protein 585A (LOC100490952), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa13543
Clone ID Related Accession (Same CDS sequence)	XM_012967563.3 , XM_004916388.4 , XM_004916388.3 , XM_012967563.2
Accession Version	XM_004916388.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 585A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916388.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916445.1
CDS	344..3814
Translation	MMENGEESYVMENDLSRPSHMELSECWVSERPEGIGSFGPVSGKMAEDQAARNSLDRSNANRQQNQWRDKAEHHQQEEEEVACQSGTVAADFSFLPGQSIAVPVPNVEKQRSREENIVAQRASEPQIVKIAEDARAQGPSFGKMEKDNMLICRPQAYNVGQQAKNIDVPTRKDIGSPEQSNQRYWGLFQCSFCNFVFQDLSELVQHQETHNQDKFQGIGQELVQLDQTESTQCEWRRYRCIVCDKTFCKQSSLVTHLRIHTGEKPYSCHVCRRKFNQRTSLTVHLRTHTGEAPFRCNKCNKSFRQQSNLTHHMKSHQRAEELDGGDWSEENRAPGFPLMYLVSEGDEFSNEVWGPKVSLIKGDKEEDSASCKRPFVCGHCFKRFTHQSNLMVHQRIHTGDRSYRCQECGKHFTRRTSLMVHLRGHTGEMPYSCQQCGKSFRQQSNLLYHMKSHAGQNDAAAGSTNKHTVKLSGQLIGPSGQYVERESPRPVSIGNSGVTRVPENQMRVYQCNQCSRWFPSTSSLLLHQKFHVEQPSNVMQSREVQVQYPIGISQPHTFTGNVLISQQEHERSQQQHFRLFSRMDGNNVPGMLNSSAPKVTPQSGSNSGYHKPGSTVEGGPIKGSFQIRGRGRPRKNIWFGSGQTAMGGFGTGKPLHKCWKCPRRFNNKSNLIVHLRIHTGEKPYQCWKCDKRFRQQSNLIQHLRNHEEFRDDDDDVPRQKKGMNKLRQSGKNFHNTAMLSSSGASQTHWSFLPFTDGNLMNQNRQLLEVSPVSQLEDAASHSDCSNDTLGNESAERSYKCGSCFKTFNHKSNLLVHERIHSGDKAYRCQECGKQFSQRTSLMVHLRTHTGEMPYSCQQCRRRFRQQSNLLYHIKTNTVHGQLKCTAENFINSVSRIDSGEDNRICTELDSIPGQAQAWMHLEPSSKTETAPALPQEAIKEEFPCPDCDKVFTHQSNLLVHQRIHSGERAYRCHECNRQFSQRTSLMIHLRTHTGEMPYACQCCGKRFRQQSNLLYHLKSHANQMAGMDVITSAEYTAPKTRGRPRKCDSNDERRAKNIIPRGKRTFKCMECPRRYNLMSNLVAHQRSHDRQPLHTCSDCGEGFLQQSYLTVHKKLHTKGDLPEHVGQFCWKQNQELMIRNPSIEEERGVLYTQHEK

Target ORF information:

RefSeq Version	XM_004916388.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 585A (LOC100490952), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916388.4

ORF Insert Sequence:

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100490952 ( XM_004916388.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916445.1 Xenopus tropicalis zinc finger protein 585A-like (LOC100490952), transcript variant X1, mRNA.

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CloneID	OXa13543
Clone ID Related Accession (Same CDS sequence)	XM_012967563.3 , XM_004916388.4 , XM_004916388.3 , XM_012967563.2
Accession Version	XM_004916388.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 585A-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916388.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916445.1
CDS	824..4294
Translation	MMENGEESYVMENDLSRPSHMELSECWVSERPEGIGSFGPVSGKMAEDQAARNSLDRSNANRQQNQWRDKAEHHQQEEEEVACQSGTVAADFSFLPGQSIAVPVPNVEKQRSREENIVAQRASEPQIVKIAEDARAQGPSFGKMEKDNMLICRPQAYNVGQQAKNIDVPTRKDIGSPEQSNQRYWGLFQCSFCNFVFQDLSELVQHQETHNQDKFQGIGQELVQLDQTESTQCEWRRYRCIVCDKTFCKQSSLVTHLRIHTGEKPYSCHVCRRKFNQRTSLTVHLRTHTGEAPFRCNKCNKSFRQQSNLTHHMKSHQRAEELDGGDWSEENRAPGFPLMYLVSEGDEFSNEVWGPKVSLIKGDKEEDSASCKRPFVCGHCFKRFTHQSNLMVHQRIHTGDRSYRCQECGKHFTRRTSLMVHLRGHTGEMPYSCQQCGKSFRQQSNLLYHMKSHAGQNDAAAGSTNKHTVKLSGQLIGPSGQYVERESPRPVSIGNSGVTRVPENQMRVYQCNQCSRWFPSTSSLLLHQKFHVEQPSNVMQSREVQVQYPIGISQPHTFTGNVLISQQEHERSQQQHFRLFSRMDGNNVPGMLNSSAPKVTPQSGSNSGYHKPGSTVEGGPIKGSFQIRGRGRPRKNIWFGSGQTAMGGFGTGKPLHKCWKCPRRFNNKSNLIVHLRIHTGEKPYQCWKCDKRFRQQSNLIQHLRNHEEFRDDDDDVPRQKKGMNKLRQSGKNFHNTAMLSSSGASQTHWSFLPFTDGNLMNQNRQLLEVSPVSQLEDAASHSDCSNDTLGNESAERSYKCGSCFKTFNHKSNLLVHERIHSGDKAYRCQECGKQFSQRTSLMVHLRTHTGEMPYSCQQCRRRFRQQSNLLYHIKTNTVHGQLKCTAENFINSVSRIDSGEDNRICTELDSIPGQAQAWMHLEPSSKTETAPALPQEAIKEEFPCPDCDKVFTHQSNLLVHQRIHSGERAYRCHECNRQFSQRTSLMIHLRTHTGEMPYACQCCGKRFRQQSNLLYHLKSHANQMAGMDVITSAEYTAPKTRGRPRKCDSNDERRAKNIIPRGKRTFKCMECPRRYNLMSNLVAHQRSHDRQPLHTCSDCGEGFLQQSYLTVHKKLHTKGDLPEHVGQFCWKQNQELMIRNPSIEEERGVLYTQHEK

Target ORF information:

RefSeq Version	XM_004916388.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 585A-like (LOC100490952), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916388.3

ORF Insert Sequence:

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100490952 ( XM_012967563.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823017.1 Xenopus tropicalis zinc finger protein 585A-like (LOC100490952), transcript variant X2, mRNA.

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CloneID	OXa13543
Clone ID Related Accession (Same CDS sequence)	XM_012967563.3 , XM_004916388.4 , XM_004916388.3 , XM_012967563.2
Accession Version	XM_012967563.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 585A-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823017.1
CDS	927..4397
Translation	MMENGEESYVMENDLSRPSHMELSECWVSERPEGIGSFGPVSGKMAEDQAARNSLDRSNANRQQNQWRDKAEHHQQEEEEVACQSGTVAADFSFLPGQSIAVPVPNVEKQRSREENIVAQRASEPQIVKIAEDARAQGPSFGKMEKDNMLICRPQAYNVGQQAKNIDVPTRKDIGSPEQSNQRYWGLFQCSFCNFVFQDLSELVQHQETHNQDKFQGIGQELVQLDQTESTQCEWRRYRCIVCDKTFCKQSSLVTHLRIHTGEKPYSCHVCRRKFNQRTSLTVHLRTHTGEAPFRCNKCNKSFRQQSNLTHHMKSHQRAEELDGGDWSEENRAPGFPLMYLVSEGDEFSNEVWGPKVSLIKGDKEEDSASCKRPFVCGHCFKRFTHQSNLMVHQRIHTGDRSYRCQECGKHFTRRTSLMVHLRGHTGEMPYSCQQCGKSFRQQSNLLYHMKSHAGQNDAAAGSTNKHTVKLSGQLIGPSGQYVERESPRPVSIGNSGVTRVPENQMRVYQCNQCSRWFPSTSSLLLHQKFHVEQPSNVMQSREVQVQYPIGISQPHTFTGNVLISQQEHERSQQQHFRLFSRMDGNNVPGMLNSSAPKVTPQSGSNSGYHKPGSTVEGGPIKGSFQIRGRGRPRKNIWFGSGQTAMGGFGTGKPLHKCWKCPRRFNNKSNLIVHLRIHTGEKPYQCWKCDKRFRQQSNLIQHLRNHEEFRDDDDDVPRQKKGMNKLRQSGKNFHNTAMLSSSGASQTHWSFLPFTDGNLMNQNRQLLEVSPVSQLEDAASHSDCSNDTLGNESAERSYKCGSCFKTFNHKSNLLVHERIHSGDKAYRCQECGKQFSQRTSLMVHLRTHTGEMPYSCQQCRRRFRQQSNLLYHIKTNTVHGQLKCTAENFINSVSRIDSGEDNRICTELDSIPGQAQAWMHLEPSSKTETAPALPQEAIKEEFPCPDCDKVFTHQSNLLVHQRIHSGERAYRCHECNRQFSQRTSLMIHLRTHTGEMPYACQCCGKRFRQQSNLLYHLKSHANQMAGMDVITSAEYTAPKTRGRPRKCDSNDERRAKNIIPRGKRTFKCMECPRRYNLMSNLVAHQRSHDRQPLHTCSDCGEGFLQQSYLTVHKKLHTKGDLPEHVGQFCWKQNQELMIRNPSIEEERGVLYTQHEK

Target ORF information:

RefSeq Version	XM_012967563.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 585A-like (LOC100490952), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967563.2

ORF Insert Sequence:

ATGATGGAAA ATGGAGAGGA ATCCTATGTT ATGGAGAACG ATCTGAGCAG GCCAAGCCAC 
ATGGAACTTT CAGAATGTTG GGTCAGTGAA AGGCCAGAAG GAATAGGGTC TTTTGGCCCT 
GTTTCTGGAA AAATGGCTGA AGACCAAGCT GCTCGGAACT CCCTGGATCG CAGTAATGCT 
AATAGACAAC AGAACCAGTG GCGAGACAAG GCAGAACACC ATCAACAAGA AGAAGAAGAA 
GTTGCATGCC AATCAGGAAC AGTTGCTGCA GATTTCAGCT TCCTTCCTGG ACAAAGTATA 
GCAGTACCTG TACCTAATGT AGAAAAACAA AGGTCTAGAG AGGAGAACAT TGTTGCTCAA 
AGGGCTTCAG AACCACAAAT AGTCAAAATT GCAGAAGATG CAAGAGCGCA GGGGCCCAGT 
TTTGGTAAAA TGGAAAAAGA CAACATGTTG ATCTGCAGAC CACAGGCATA TAATGTTGGC 
CAGCAGGCTA AGAATATAGA TGTACCCACA AGAAAAGACA TTGGATCTCC AGAACAGTCA 
AATCAGAGAT ACTGGGGCTT GTTTCAATGT TCCTTCTGCA ATTTTGTTTT TCAAGACCTG 
TCTGAACTGG TGCAGCACCA GGAAACTCAC AATCAGGACA AGTTTCAAGG CATTGGCCAA 
GAACTTGTGC AGCTGGATCA GACTGAGAGT ACTCAGTGTG AGTGGAGGAG ATACCGTTGC 
ATTGTGTGCG ATAAGACTTT CTGCAAACAG TCCTCACTAG TCACTCACCT TCGTATACAC 
ACAGGTGAGA AACCGTATTC TTGTCACGTA TGTAGAAGGA AATTCAATCA GCGCACAAGC 
CTAACTGTCC ACCTTAGGAC ACATACTGGC GAGGCTCCGT TTCGCTGCAA TAAGTGCAAT 
AAGAGTTTCA GGCAGCAATC TAACCTTACA CATCACATGA AGAGCCACCA AAGAGCTGAG 
GAGCTTGATG GAGGAGACTG GAGTGAAGAA AACCGAGCCC CTGGATTTCC ATTGATGTAT 
CTTGTTTCTG AAGGGGATGA ATTTAGTAAT GAAGTATGGG GTCCCAAGGT ATCTTTAATC 
AAAGGGGATA AGGAAGAAGA CTCTGCTTCT TGCAAAAGAC CTTTTGTCTG TGGACACTGT 
TTTAAGCGCT TCACGCACCA GTCCAACCTT ATGGTTCATC AGCGCATTCA CACAGGGGAT 
CGATCTTACC GTTGCCAAGA ATGTGGCAAA CACTTTACAA GGAGGACTAG CTTGATGGTT 
CACTTGAGAG GGCACACCGG TGAGATGCCG TACTCCTGTC AGCAGTGTGG TAAAAGTTTT 
CGGCAGCAAT CTAATCTTCT CTACCACATG AAGAGTCATG CCGGCCAAAA CGATGCAGCT 
GCAGGCAGTA CAAATAAACA TACAGTGAAA CTATCGGGGC AGTTGATTGG GCCCTCAGGC 
CAGTATGTGG AGAGAGAGAG TCCACGTCCA GTAAGCATTG GCAATAGTGG AGTCACTAGA 
GTTCCTGAGA ACCAAATGAG GGTTTATCAG TGCAACCAGT GTTCAAGATG GTTTCCTAGT 
ACTTCTAGTC TCTTGCTTCA TCAAAAGTTT CACGTGGAAC AGCCAAGTAA CGTTATGCAG 
AGCAGAGAAG TTCAGGTTCA GTACCCCATC GGTATAAGCC AACCACATAC ATTCACAGGG 
AATGTGCTGA TTTCCCAGCA AGAACATGAA AGGAGTCAAC AGCAACATTT CAGACTTTTC 
TCTCGGATGG ATGGAAACAA TGTTCCAGGA ATGCTTAATA GCTCTGCACC AAAAGTCACC 
CCACAGTCAG GATCCAACTC TGGGTATCAT AAACCTGGAA GTACAGTAGA AGGAGGACCT 
ATTAAGGGAA GTTTCCAAAT AAGAGGAAGA GGAAGACCTA GGAAGAATAT CTGGTTTGGG 
TCAGGACAAA CAGCCATGGG AGGTTTTGGA ACAGGAAAAC CTCTTCATAA ATGTTGGAAA 
TGCCCTCGAC GTTTCAATAA TAAATCAAAT TTAATCGTCC ATTTACGAAT TCACACAGGG 
GAAAAGCCTT ACCAGTGCTG GAAGTGTGAC AAGCGGTTTC GCCAACAGTC AAACCTGATC 
CAGCATCTCC GAAACCATGA GGAATTCCGT GATGATGATG ATGATGTTCC AAGACAAAAG 
AAGGGAATGA ATAAACTAAG GCAAAGTGGG AAGAATTTTC ACAATACAGC GATGCTTTCC 
TCATCTGGTG CATCTCAAAC CCACTGGAGT TTTCTTCCTT TTACTGATGG AAACCTCATG 
AATCAAAACA GACAGTTACT TGAGGTGTCC CCGGTCTCGC AGTTAGAGGA CGCTGCATCC 
CACAGTGACT GCAGCAATGA CACCTTAGGG AATGAATCCG CTGAGAGGTC ATATAAGTGT 
GGCAGCTGCT TTAAGACATT TAACCACAAA TCCAACCTAT TAGTCCATGA GCGTATCCAC 
TCAGGAGACA AGGCCTATCG TTGTCAGGAA TGTGGGAAGC AATTTAGTCA GCGCACTAGT 
TTAATGGTGC ATCTCCGAAC TCATACAGGG GAGATGCCCT ACTCCTGCCA GCAATGCAGG 
AGGCGCTTCC GTCAACAGTC TAATTTACTT TACCACATAA AGACCAACAC AGTCCATGGG 
CAGCTTAAAT GTACAGCCGA GAACTTTATT AACTCAGTTT CTAGAATTGA TTCTGGGGAG 
GATAATAGAA TTTGTACTGA GTTGGATTCA ATCCCTGGGC AAGCGCAGGC TTGGATGCAT 
TTGGAACCAA GTTCTAAGAC TGAAACTGCA CCAGCCCTGC CCCAGGAGGC AATAAAGGAG 
GAGTTTCCCT GCCCTGACTG TGACAAGGTG TTTACTCATC AGTCAAACCT GCTAGTCCAC 
CAACGAATCC ATTCTGGAGA AAGGGCATAC CGATGCCATG AGTGCAACCG TCAGTTCAGC 
CAGCGCACCA GCCTGATGAT CCATTTGCGC ACTCACACCG GGGAGATGCC CTATGCTTGC 
CAGTGTTGTG GCAAGAGATT CCGCCAACAA TCAAACCTGT TGTATCACTT AAAAAGCCAT 
GCCAACCAAA TGGCTGGCAT GGATGTTATT ACAAGTGCAG AGTATACAGC TCCAAAAACA 
AGAGGCCGAC CCCGCAAATG TGATTCTAAT GATGAGAGGA GAGCAAAAAA TATCATTCCG 
AGGGGCAAGC GTACCTTCAA GTGCATGGAG TGCCCAAGGC GCTATAATTT AATGTCCAAC 
TTAGTTGCTC ATCAGAGATC CCATGACCGC CAACCACTGC ATACGTGTTC AGATTGTGGG 
GAAGGTTTCC TTCAGCAGAG TTACCTCACC GTGCACAAGA AACTCCACAC CAAGGGAGAC 
CTTCCTGAAC ACGTTGGGCA GTTCTGCTGG AAACAGAATC AAGAGCTGAT GATCAGAAAT 
CCAAGCATAG AGGAAGAGAG AGGAGTACTT TATACACAAC ATGAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100490952 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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