ddx60 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx60
Entrez Gene ID	100489902
Full Name	DEXD/H-box helicase 60
General protein information	Preferred Names DEXD/H-box helicase 60 Names probable ATP-dependent RNA helicase DDX60 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031895461.1	XP_031751321.1	probable ATP-dependent RNA helicase DDX60 isoform X4
XM_031895458.1	XP_031751318.1	probable ATP-dependent RNA helicase DDX60 isoform X1
XM_018095853.1	XP_017951342.1	probable ATP-dependent RNA helicase DDX60
XM_031895459.1	XP_031751319.1	probable ATP-dependent RNA helicase DDX60 isoform X2
XM_031895462.1	XP_031751322.1	probable ATP-dependent RNA helicase DDX60 isoform X4
XM_031895460.1	XP_031751320.1	probable ATP-dependent RNA helicase DDX60 isoform X3

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	C28H8.3	NP_498283.1
Xenopus tropicalis (tropical clawed frog)	ddx60	XP_002933376.2
Homo sapiens (human)	DDX60	NP_060101.3
Mus musculus (house mouse)	Ddx60	NP_001074684.1
Gallus gallus (chicken)	DDX60	XP_004940975.1
	DDX60	XP_001082147.1
Canis lupus familiaris (dog)	DDX60	XP_532716.4
Pan troglodytes (chimpanzee)	DDX60	XP_003310648.1

Gene Ontology

The following ddx60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa41937	XM_018095853.1 Latest version!	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa49814	XM_031895458.1 Latest version!	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49815	XM_031895459.1 Latest version!	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49816	XM_031895460.1 Latest version!	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49817	XM_031895462.1 Latest version!	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49817	XM_031895461.1 Latest version!	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx60 ( XM_018095853.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951342.1 Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), mRNA.

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CloneID	OXa41937
Clone ID Related Accession (Same CDS sequence)	XM_018095853.1
Accession Version	XM_018095853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX60
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AAGGGTCTCA TTTGGAAAAG ATCTCACTGG ACTCTCAAAC AGAGATTTCT 
GTATCACATG AAGCAGAACC AAAGACTAAA AATGAATCCC GAATGAAAAA TGATGACGCC 
CTTCGTGACT TGTTCACTTT TACAGTGCAG AAACTCTCAG GAGCTGTCTT TGCCAGCCTG 
CTTAATGATT ATGTGGAATC TGAATTCTTC TTGATTGATG GAGATTCCTT ACTGCTAACT 
TGTGTTTTGG ATCAGTCACT GAAGGCAGGG CAGCATCTGC ATTTCTTTTA CTTGGTAGAA 
TCCTACTTGC ATAAACTGAC CAACCGGGGA GCAAAATATG CTGTTGTGTT TTTTGAGGAT 
ATGGAGTTTT TCTACCATGA CAGAGTCGAC CTTATCTCTC TACGGACCCA ACTGAAACTG 
CACTTGGAAC ACAACACAGA CACAGTCATA TACACCTTTT CCAACTTTTT GTCTGCTCAG 
TGGAACACAT TTCTGATTGA TCACCATCCC TACTTTCTAA TGGTTTGTGA TGAAGGTGTT 
ACCCTGTCTC AAACATATTT CTTCAGTATG CTTATGCTAA GTGCCCTGGG GAAAAAAATA 
GACCTCGTAC TCACGTCAGG ACAAGAGTTC GATCAGCTCC GAGTGTATGG ATACCATATC 
AATGCCACCC ACCATCATAA CTTATATCCC ATAAAGAAAC AGAAGCAGTT TCTTCAAGAT 
TTGGTAGATC CATGTGAAAA GTTGTACAGA AAGCGTGCAC AGAAGCTGAA TTTCAGTAAC 
ACCATAAACG AGATTGAAAA GAGTATAACA TTACTTTTTA ACAATGGGGA TAAGGCTTTA 
GACATAAGAA GCATCATGTG TATTCTGTCC TGCTCTGCAG TGCTGACAAT ATATGGTAAA 
AAAGCAACAC ATGGAGAATG TCAGGCCTCA CAGGTACAGG ATGACAGTGA CCTGACCTTG 
GAAGAAGTGG CTGACCTATG CAGAATGTAC TGTCTGAGTG TGGCCATATT GCTGGTTTTG 
CCCCTTTCCC AGAGAATGAA ATCTAGGAAC ATCCATGCAG ATTGGAACAG CCCAGTATTA 
GCATGTTTAG ATTGGCAAAT TATATGTGAG CACATTATTC TGAAGAAGTT TTCCAACACC 
TCAAACTGGA AGATAAATTG GACACACATT GCTGATCTAA GTGATGGTTT GCTGTTGAAA 
AACATTGCAT ATTACTATGA AAAAGAGAAC AATACAGGCT CCAAGTTGAT ATTCGGGAAG 
GCAATAGAGG AGAAGTTTTC ATTTCTCTGG AACTCCATTT TGGAAATTAA TCCACATAGT 
GAACAAATAC AATCTTATGA CGTCAGAACA ACATCACGTC CTTTCCTTTC AAAGGCTTCC 
TCAATAATTG TGAGAGAGGA GAACATGCCA ACTATAGGTC TTCTACGAGC AAAATCTGAC 
ATTGTTGAAG AGTATGCTGG GGATTTTTTG GATGAATTAC CATTTTTAAG CAGTATTGAA 
CATAAAGAGA CCCCAGTCAC TCGAGGGAAA TATTTTGATG AACTTACACA CTGGCATTCT 
GGACGTCCAC TAAGTGATGA TTATGACAGA ACAAAGGGAG ATGAGGCAGC ATTGAAAGAT 
GAACGTGCTC TCAGAGATTA CCAAAAGCTG CAAACTCTTC ACCGTTTTAG CGGGCAATCT 
TTAGTCTCCT CATCATCCAA GACAATTGTA ATACAGAATG ATGACCCTGA AAAGAAGGGG 
GAGAAAGAAA AGGTTATTGT TAAGAAAAAG CAACCACAGA AGTCTAAAAA AGATCTAATC 
ATAGAGGAAA ACCAGAAAAG AATGAATGCC AAAGAGGAAA CTAAAGACGC TGAACAGTGG 
AAATCAGTGG CATCTTCAAT TGAAAAAGAA ATGAGAGCAA ATTTTTTTCC TGGCTTAAAA 
AAACTGGAAA GTTTCATAAA AAATACCCAA ACCCGCTCAG TGAAGCTTCT TGCAGAACTA 
TTAGGATTAA GAATTTCCTT TAAGGTGTGG GTCGAGCACT GCTGCACAGA CATGTCCTCA 
GAAGAGGAGG ACGATTTAAA TATTGCTGTG GAAATCATGA AGAAGATACA AACACTTCAT 
TATAAATACA AGGACATGCT GACTAATGAG GATCAACAAC TAATAGCTCA ATGTCTTAGT 
TATCTGGGAT TTGAGAACTT GGCATGCACA TTGCCGCGCG CTAAGGTAAC CAGTGCCAGA 
GAGGACAAGA AGAAGGCAGA GTGCATGGTT GGAGTTGGAG CAGCTCGTTT CCAATTGCAG 
TTTATGAGTC CATACTTGGT AAGAGAGGAG AGGACAGATC CTGATCCAAG AGTACAACAC 
TTTATACCGG ACACATGGCA GCGTAAACTT CTTGATGTTG TTGACAATAA TGAGTCTGCG 
GTGATAGTTG CCCCTACATC TTCAGGAAAG ACTTACGCTT CGTACTACTG CATGCAGAAA 
GTTCTGAGTC AAGGCCATGA AGGTGTGGTT GTGTATGTTT CACCAACAAA GGCTCTTGTA 
AACCAGGTGG TAACCACTGT AATGAATCAG TTCAACAAAA ATTTGCCCAA TGGAGTGGCT 
CTGTGTGGTG TTTTCACAAG GGATTACCGT ACTGATGCAT TAAATTCTCA GATATTAGTA 
ACAGTGCCTC AGTGTCTTGA GATTCTTCTC TTGTCGCCAC ACCGTCAGGA ATGGGCTAAA 
AGGATTAAGT ATGTAATATT TGATGAGATT CATTGCCTGG GTGGAGAAAT AGGTGCAGAA 
GTATGGGAAC ATCTTCTAGT GATGATTCGC TGTCCATTTC TTGCTTTGTC TGCAACAATA 
AGTAACCCGG AACATCTAAC TGAATGGCTG CAATCAGTCA AATTTTACTG GCAAAAAAGA 
GATAAATTAA TAGAGGAAAA CTGGACTGAG AAAGGAGCAA AGGCAAAAGC CTATAAGAAG 
AAAGAAAAAA TGTCTTACAG AGTTCATCTG GTGCTTTATA ACAAGAGATA CAATGATCTG 
GAAAAATATG TGTGCTCTGT GCAAAACTCC AATGTATCGT TTGAGCACTA TCACCCATGT 
GCTGCCTTAA CCACGGACCA CTTGGAGAAA TATGGAATTC CAAATGACCT GTCCTGCTCC 
CCGAGGGAGA GTTTACAACT GTATGACACT ATGGTGGATG TGTGGCCTGA ATGGGCAAGG 
AGAGAGGAAC TCGATCCTGA AGAATATGCA CATTTCAAGG ACAAAATCAT CATAACAAAA 
AATGATACAA AAATATATGA GGAAGAACTT AAAAAAGAAT TAATTGACTG GATTAAGCAA 
GGAAACCAGG AAAAGGTTTC CCAACTATTG AAGTATCTTC AGCCACCAAA ATCATCTTGT 
AATGTGGACT GTGTTGCCTA CTTTCCAAAG CTTGTTGAAA AACTGAAAGA AGAGCAAAAG 
CTGCCAGCTC TTCTTTTTGC ATTTAACATT GGACTTGTGG AACATCTCGC ACAGGAAACA 
GCCATATTTC TAAAAAACAA GCAAGACAAT AAGACAAAAC TTCTGACTGA GAAAAAAAGC 
CAAAAACTTA TCACCAAAGC AAAAAGACTG GAAAAAGCAA TAGCAAAAAA TGAACCCTCA 
AAAGTAAACG ACGCTTTCTT AGCCAATGTT GCCAATCATA AGAGTATAGA GCAAGTACTT 
ATAAAGGACA AAGAAATCCC ACCAGACTGT ACTTATGCTG ATACCAGAGC CGTGAACAAT 
GAGTTACTCT GGGAGGTGTT CAGACAAACA AGTCGATCAA GAGATGCCAA GTGTCTGAGG 
TTTCTGGCAG AAAGAGGTGT GGGCTACCAT CATGCCTCTT TGGATGTTAA AGGAAGAAGA 
CAAGTGGAGA TGCTCTTCAG GATGGGATAC CTACGGGTTG TTACTGCAAC TGGATCTCTT 
GCCTTGGGAA TCAACATGCC GTGTAAATCT GTTGTGTTTG TTCAAGATTC ACTTTACCTG 
GATGCATTAA ATTACAGACA GATGTCAGGA CGTGCAGGAA GGCGAGGACA GGATGTACTG 
GGAAATGTAT ACTTTTTCAC CATTCCTATG CCTAAAGTGA AACGGCTTAT GAAATCAGAT 
GTCCCTAACT TAAAAGGACA GTTCCCTTTA GGCATCTCTT TTGTCCTACG ACTGATGTTA 
TTGGCTGCCA AAGCTGATCA CAAAGAAGAT GCCAGTGCTA AGGCTTTGTC AGTACTGAAG 
CACTCCCTGA TGGCGTTCAA AAAACCTTCT GAGACCAAAA TGTTGAAGCT CTACTTTCTG 
TTCTGCATAC ACTTCTTAAT CCATGAGGGC TATTTGGATC AACATTGCAA CCCTTTGGAC 
TTTACCGGGC TGGTTACACA TTTACATTAC CATGAACCAT CAAATTTTGT GTTTGTCAGT 
TTTCTGGAAA ATGGTTTATT TCACCGGCTG TGCCAGAGAA ACACTAAAGG TTCAAATAGG 
TTTCCACAAT CTGTGATGGA AACCCTGGTG CTGGTATTGG CTAACTTGTT TGGAAGAAGC 
TACCTTTTAC CATCCATGCT AGAATTAAAG GGAACATTTA AACAGTCAAA GGTCTTTCTG 
GAAGGTCTAC CAGATGATTT TGCTGCAGCT CTTGAAGACT ACAATTCCAA AATATCAGCA 
GTCTTTGGGC AGTATCTTCT TGCTGTTTCT CATCTGGCAA ATTATGAACA AGAATACAAG 
CTTCCTCTAT CACAAATCAA CTTCTCAGGG GAGGAATGTG AGGATTCTGA ACTTGTAAAC 
CACTTGATGA ACTGCACCAA AAGGAGAAGT GCCATAACCC CCTTTGCCTG CCTGTCTGGA 
AATACAGATC ATGACTTAAC TTTTGCAGCA CATTTGGACA GTATTTTGTT GCAAACTAGT 
CATGTGAGTG AAAAACACAT TCCTCGCCTG CATTTGGAGA ACACAGATGC TTGTGGTAGA 
AAACTGTTAC TAAATGCCTA TGCACTGGAT TTCTTCAAGC ACAGCTCCAT AGATGCACTG 
GAAACTGATA ATGGGTTTCA CAAAGGAGAT GCTTTTTACA AGTTACGAGA TTTTTCTTTA 
TGCATTGCAA CTATAAGAGT TTCTTTAAAG GAAATGTGTG AAGATGAGGA TGACCCCGTT 
GTGTTAGCTT TCGAGCAGCT GAATACCCTG TACAGAGAGA AACTGGAGAC TGCTTATCCC 
AGGCGCAGAA TCTTTTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951342.1
CDS	236..5419
Translation	MATQGSHLEKISLDSQTEISVSHEAEPKTKNESRMKNDDALRDLFTFTVQKLSGAVFASLLNDYVESEFFLIDGDSLLLTCVLDQSLKAGQHLHFFYLVESYLHKLTNRGAKYAVVFFEDMEFFYHDRVDLISLRTQLKLHLEHNTDTVIYTFSNFLSAQWNTFLIDHHPYFLMVCDEGVTLSQTYFFSMLMLSALGKKIDLVLTSGQEFDQLRVYGYHINATHHHNLYPIKKQKQFLQDLVDPCEKLYRKRAQKLNFSNTINEIEKSITLLFNNGDKALDIRSIMCILSCSAVLTIYGKKATHGECQASQVQDDSDLTLEEVADLCRMYCLSVAILLVLPLSQRMKSRNIHADWNSPVLACLDWQIICEHIILKKFSNTSNWKINWTHIADLSDGLLLKNIAYYYEKENNTGSKLIFGKAIEEKFSFLWNSILEINPHSEQIQSYDVRTTSRPFLSKASSIIVREENMPTIGLLRAKSDIVEEYAGDFLDELPFLSSIEHKETPVTRGKYFDELTHWHSGRPLSDDYDRTKGDEAALKDERALRDYQKLQTLHRFSGQSLVSSSSKTIVIQNDDPEKKGEKEKVIVKKKQPQKSKKDLIIEENQKRMNAKEETKDAEQWKSVASSIEKEMRANFFPGLKKLESFIKNTQTRSVKLLAELLGLRISFKVWVEHCCTDMSSEEEDDLNIAVEIMKKIQTLHYKYKDMLTNEDQQLIAQCLSYLGFENLACTLPRAKVTSAREDKKKAECMVGVGAARFQLQFMSPYLVREERTDPDPRVQHFIPDTWQRKLLDVVDNNESAVIVAPTSSGKTYASYYCMQKVLSQGHEGVVVYVSPTKALVNQVVTTVMNQFNKNLPNGVALCGVFTRDYRTDALNSQILVTVPQCLEILLLSPHRQEWAKRIKYVIFDEIHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKFYWQKRDKLIEENWTEKGAKAKAYKKKEKMSYRVHLVLYNKRYNDLEKYVCSVQNSNVSFEHYHPCAALTTDHLEKYGIPNDLSCSPRESLQLYDTMVDVWPEWARREELDPEEYAHFKDKIIITKNDTKIYEEELKKELIDWIKQGNQEKVSQLLKYLQPPKSSCNVDCVAYFPKLVEKLKEEQKLPALLFAFNIGLVEHLAQETAIFLKNKQDNKTKLLTEKKSQKLITKAKRLEKAIAKNEPSKVNDAFLANVANHKSIEQVLIKDKEIPPDCTYADTRAVNNELLWEVFRQTSRSRDAKCLRFLAERGVGYHHASLDVKGRRQVEMLFRMGYLRVVTATGSLALGINMPCKSVVFVQDSLYLDALNYRQMSGRAGRRGQDVLGNVYFFTIPMPKVKRLMKSDVPNLKGQFPLGISFVLRLMLLAAKADHKEDASAKALSVLKHSLMAFKKPSETKMLKLYFLFCIHFLIHEGYLDQHCNPLDFTGLVTHLHYHEPSNFVFVSFLENGLFHRLCQRNTKGSNRFPQSVMETLVLVLANLFGRSYLLPSMLELKGTFKQSKVFLEGLPDDFAAALEDYNSKISAVFGQYLLAVSHLANYEQEYKLPLSQINFSGEECEDSELVNHLMNCTKRRSAITPFACLSGNTDHDLTFAAHLDSILLQTSHVSEKHIPRLHLENTDACGRKLLLNAYALDFFKHSSIDALETDNGFHKGDAFYKLRDFSLCIATIRVSLKEMCEDEDDPVVLAFEQLNTLYREKLETAYPRRRIFYA

Target ORF information:

RefSeq Version	XM_018095853.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095853.1

ORF Insert Sequence:

ATGGCCACAC AAGGGTCTCA TTTGGAAAAG ATCTCACTGG ACTCTCAAAC AGAGATTTCT 
GTATCACATG AAGCAGAACC AAAGACTAAA AATGAATCCC GAATGAAAAA TGATGACGCC 
CTTCGTGACT TGTTCACTTT TACAGTGCAG AAACTCTCAG GAGCTGTCTT TGCCAGCCTG 
CTTAATGATT ATGTGGAATC TGAATTCTTC TTGATTGATG GAGATTCCTT ACTGCTAACT 
TGTGTTTTGG ATCAGTCACT GAAGGCAGGG CAGCATCTGC ATTTCTTTTA CTTGGTAGAA 
TCCTACTTGC ATAAACTGAC CAACCGGGGA GCAAAATATG CTGTTGTGTT TTTTGAGGAT 
ATGGAGTTTT TCTACCATGA CAGAGTCGAC CTTATCTCTC TACGGACCCA ACTGAAACTG 
CACTTGGAAC ACAACACAGA CACAGTCATA TACACCTTTT CCAACTTTTT GTCTGCTCAG 
TGGAACACAT TTCTGATTGA TCACCATCCC TACTTTCTAA TGGTTTGTGA TGAAGGTGTT 
ACCCTGTCTC AAACATATTT CTTCAGTATG CTTATGCTAA GTGCCCTGGG GAAAAAAATA 
GACCTCGTAC TCACGTCAGG ACAAGAGTTC GATCAGCTCC GAGTGTATGG ATACCATATC 
AATGCCACCC ACCATCATAA CTTATATCCC ATAAAGAAAC AGAAGCAGTT TCTTCAAGAT 
TTGGTAGATC CATGTGAAAA GTTGTACAGA AAGCGTGCAC AGAAGCTGAA TTTCAGTAAC 
ACCATAAACG AGATTGAAAA GAGTATAACA TTACTTTTTA ACAATGGGGA TAAGGCTTTA 
GACATAAGAA GCATCATGTG TATTCTGTCC TGCTCTGCAG TGCTGACAAT ATATGGTAAA 
AAAGCAACAC ATGGAGAATG TCAGGCCTCA CAGGTACAGG ATGACAGTGA CCTGACCTTG 
GAAGAAGTGG CTGACCTATG CAGAATGTAC TGTCTGAGTG TGGCCATATT GCTGGTTTTG 
CCCCTTTCCC AGAGAATGAA ATCTAGGAAC ATCCATGCAG ATTGGAACAG CCCAGTATTA 
GCATGTTTAG ATTGGCAAAT TATATGTGAG CACATTATTC TGAAGAAGTT TTCCAACACC 
TCAAACTGGA AGATAAATTG GACACACATT GCTGATCTAA GTGATGGTTT GCTGTTGAAA 
AACATTGCAT ATTACTATGA AAAAGAGAAC AATACAGGCT CCAAGTTGAT ATTCGGGAAG 
GCAATAGAGG AGAAGTTTTC ATTTCTCTGG AACTCCATTT TGGAAATTAA TCCACATAGT 
GAACAAATAC AATCTTATGA CGTCAGAACA ACATCACGTC CTTTCCTTTC AAAGGCTTCC 
TCAATAATTG TGAGAGAGGA GAACATGCCA ACTATAGGTC TTCTACGAGC AAAATCTGAC 
ATTGTTGAAG AGTATGCTGG GGATTTTTTG GATGAATTAC CATTTTTAAG CAGTATTGAA 
CATAAAGAGA CCCCAGTCAC TCGAGGGAAA TATTTTGATG AACTTACACA CTGGCATTCT 
GGACGTCCAC TAAGTGATGA TTATGACAGA ACAAAGGGAG ATGAGGCAGC ATTGAAAGAT 
GAACGTGCTC TCAGAGATTA CCAAAAGCTG CAAACTCTTC ACCGTTTTAG CGGGCAATCT 
TTAGTCTCCT CATCATCCAA GACAATTGTA ATACAGAATG ATGACCCTGA AAAGAAGGGG 
GAGAAAGAAA AGGTTATTGT TAAGAAAAAG CAACCACAGA AGTCTAAAAA AGATCTAATC 
ATAGAGGAAA ACCAGAAAAG AATGAATGCC AAAGAGGAAA CTAAAGACGC TGAACAGTGG 
AAATCAGTGG CATCTTCAAT TGAAAAAGAA ATGAGAGCAA ATTTTTTTCC TGGCTTAAAA 
AAACTGGAAA GTTTCATAAA AAATACCCAA ACCCGCTCAG TGAAGCTTCT TGCAGAACTA 
TTAGGATTAA GAATTTCCTT TAAGGTGTGG GTCGAGCACT GCTGCACAGA CATGTCCTCA 
GAAGAGGAGG ACGATTTAAA TATTGCTGTG GAAATCATGA AGAAGATACA AACACTTCAT 
TATAAATACA AGGACATGCT GACTAATGAG GATCAACAAC TAATAGCTCA ATGTCTTAGT 
TATCTGGGAT TTGAGAACTT GGCATGCACA TTGCCGCGCG CTAAGGTAAC CAGTGCCAGA 
GAGGACAAGA AGAAGGCAGA GTGCATGGTT GGAGTTGGAG CAGCTCGTTT CCAATTGCAG 
TTTATGAGTC CATACTTGGT AAGAGAGGAG AGGACAGATC CTGATCCAAG AGTACAACAC 
TTTATACCGG ACACATGGCA GCGTAAACTT CTTGATGTTG TTGACAATAA TGAGTCTGCG 
GTGATAGTTG CCCCTACATC TTCAGGAAAG ACTTACGCTT CGTACTACTG CATGCAGAAA 
GTTCTGAGTC AAGGCCATGA AGGTGTGGTT GTGTATGTTT CACCAACAAA GGCTCTTGTA 
AACCAGGTGG TAACCACTGT AATGAATCAG TTCAACAAAA ATTTGCCCAA TGGAGTGGCT 
CTGTGTGGTG TTTTCACAAG GGATTACCGT ACTGATGCAT TAAATTCTCA GATATTAGTA 
ACAGTGCCTC AGTGTCTTGA GATTCTTCTC TTGTCGCCAC ACCGTCAGGA ATGGGCTAAA 
AGGATTAAGT ATGTAATATT TGATGAGATT CATTGCCTGG GTGGAGAAAT AGGTGCAGAA 
GTATGGGAAC ATCTTCTAGT GATGATTCGC TGTCCATTTC TTGCTTTGTC TGCAACAATA 
AGTAACCCGG AACATCTAAC TGAATGGCTG CAATCAGTCA AATTTTACTG GCAAAAAAGA 
GATAAATTAA TAGAGGAAAA CTGGACTGAG AAAGGAGCAA AGGCAAAAGC CTATAAGAAG 
AAAGAAAAAA TGTCTTACAG AGTTCATCTG GTGCTTTATA ACAAGAGATA CAATGATCTG 
GAAAAATATG TGTGCTCTGT GCAAAACTCC AATGTATCGT TTGAGCACTA TCACCCATGT 
GCTGCCTTAA CCACGGACCA CTTGGAGAAA TATGGAATTC CAAATGACCT GTCCTGCTCC 
CCGAGGGAGA GTTTACAACT GTATGACACT ATGGTGGATG TGTGGCCTGA ATGGGCAAGG 
AGAGAGGAAC TCGATCCTGA AGAATATGCA CATTTCAAGG ACAAAATCAT CATAACAAAA 
AATGATACAA AAATATATGA GGAAGAACTT AAAAAAGAAT TAATTGACTG GATTAAGCAA 
GGAAACCAGG AAAAGGTTTC CCAACTATTG AAGTATCTTC AGCCACCAAA ATCATCTTGT 
AATGTGGACT GTGTTGCCTA CTTTCCAAAG CTTGTTGAAA AACTGAAAGA AGAGCAAAAG 
CTGCCAGCTC TTCTTTTTGC ATTTAACATT GGACTTGTGG AACATCTCGC ACAGGAAACA 
GCCATATTTC TAAAAAACAA GCAAGACAAT AAGACAAAAC TTCTGACTGA GAAAAAAAGC 
CAAAAACTTA TCACCAAAGC AAAAAGACTG GAAAAAGCAA TAGCAAAAAA TGAACCCTCA 
AAAGTAAACG ACGCTTTCTT AGCCAATGTT GCCAATCATA AGAGTATAGA GCAAGTACTT 
ATAAAGGACA AAGAAATCCC ACCAGACTGT ACTTATGCTG ATACCAGAGC CGTGAACAAT 
GAGTTACTCT GGGAGGTGTT CAGACAAACA AGTCGATCAA GAGATGCCAA GTGTCTGAGG 
TTTCTGGCAG AAAGAGGTGT GGGCTACCAT CATGCCTCTT TGGATGTTAA AGGAAGAAGA 
CAAGTGGAGA TGCTCTTCAG GATGGGATAC CTACGGGTTG TTACTGCAAC TGGATCTCTT 
GCCTTGGGAA TCAACATGCC GTGTAAATCT GTTGTGTTTG TTCAAGATTC ACTTTACCTG 
GATGCATTAA ATTACAGACA GATGTCAGGA CGTGCAGGAA GGCGAGGACA GGATGTACTG 
GGAAATGTAT ACTTTTTCAC CATTCCTATG CCTAAAGTGA AACGGCTTAT GAAATCAGAT 
GTCCCTAACT TAAAAGGACA GTTCCCTTTA GGCATCTCTT TTGTCCTACG ACTGATGTTA 
TTGGCTGCCA AAGCTGATCA CAAAGAAGAT GCCAGTGCTA AGGCTTTGTC AGTACTGAAG 
CACTCCCTGA TGGCGTTCAA AAAACCTTCT GAGACCAAAA TGTTGAAGCT CTACTTTCTG 
TTCTGCATAC ACTTCTTAAT CCATGAGGGC TATTTGGATC AACATTGCAA CCCTTTGGAC 
TTTACCGGGC TGGTTACACA TTTACATTAC CATGAACCAT CAAATTTTGT GTTTGTCAGT 
TTTCTGGAAA ATGGTTTATT TCACCGGCTG TGCCAGAGAA ACACTAAAGG TTCAAATAGG 
TTTCCACAAT CTGTGATGGA AACCCTGGTG CTGGTATTGG CTAACTTGTT TGGAAGAAGC 
TACCTTTTAC CATCCATGCT AGAATTAAAG GGAACATTTA AACAGTCAAA GGTCTTTCTG 
GAAGGTCTAC CAGATGATTT TGCTGCAGCT CTTGAAGACT ACAATTCCAA AATATCAGCA 
GTCTTTGGGC AGTATCTTCT TGCTGTTTCT CATCTGGCAA ATTATGAACA AGAATACAAG 
CTTCCTCTAT CACAAATCAA CTTCTCAGGG GAGGAATGTG AGGATTCTGA ACTTGTAAAC 
CACTTGATGA ACTGCACCAA AAGGAGAAGT GCCATAACCC CCTTTGCCTG CCTGTCTGGA 
AATACAGATC ATGACTTAAC TTTTGCAGCA CATTTGGACA GTATTTTGTT GCAAACTAGT 
CATGTGAGTG AAAAACACAT TCCTCGCCTG CATTTGGAGA ACACAGATGC TTGTGGTAGA 
AAACTGTTAC TAAATGCCTA TGCACTGGAT TTCTTCAAGC ACAGCTCCAT AGATGCACTG 
GAAACTGATA ATGGGTTTCA CAAAGGAGAT GCTTTTTACA AGTTACGAGA TTTTTCTTTA 
TGCATTGCAA CTATAAGAGT TTCTTTAAAG GAAATGTGTG AAGATGAGGA TGACCCCGTT 
GTGTTAGCTT TCGAGCAGCT GAATACCCTG TACAGAGAGA AACTGGAGAC TGCTTATCCC 
AGGCGCAGAA TCTTTTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx60 ( XM_031895458.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751318.1 Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X1, mRNA.

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CloneID	OXa49814
Clone ID Related Accession (Same CDS sequence)	XM_031895458.1
Accession Version	XM_031895458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAC AAGGATCTTA TTTAGGACAG AAGTCACTGG AGTCTCAAAA AGAACCAGAT 
CCTGAATCTT CTGTACCATG TGACCCAGAA CCAAAAAACA ATGCTGAATC CCAGGTGAAA 
AGTTCTGAAT TCCCCCATGG CATGTACTTA TATGTAATGG AGAAACTCTC AAGAGCTGTC 
TTTGCCAGCC TGCTTAATGA TTATGTGGAA TCTGAATTCT TCTTGATTGA TGGAGATTCC 
TTACTGCTAA CTTGTGTTTT GGATCAGTCA CTGAAGGCAG GGCAGCATCT GCATTTCTTT 
TACTTGGTAG AATCCTACTT GCATAAACTG ACCAACCGGG GAGCAAAATA TGCTGTTGTG 
TTTTTTGAGG ATATGGAGTT TTTCTACCAT GACAGAGTCG ACCTTATCTC TCTACGGACC 
CAACTGAAAC TGCACTTGGA ACACAACACA GACACAGTCA TATACACCTT TTCCAACTTT 
TTGTCTGCTC AGTGGAACAC ATTTCTGATT GATCACCATC CCTACTTTCT AATGGTTTGT 
GATGAAGGTG TTACCCTGTC TCAAACATAT TTCTTCAGTA TGCTTATGCT AAGTGCCCTG 
GGGAAAAAAA TAGACCTCGT ACTCACGTCA GGACAAGAGT TCGATCAGCT CCGAGTGTAT 
GGATACCATA TCAATGCCAC CCACCATCAT AACTTATATC CCATAAAGAA ACAGAAGCAG 
TTTCTTCAAG ATTTGGTAGA TCCATGTGAA AAGTTGTACA GAAAGCGTGC ACAGAAGCTG 
AATTTCAGTA ACACCATAAA CGAGATTGAA AAGAGTATAA CATTACTTTT TAACAATGGG 
GATAAGGCTT TAGACATAAG AAGCATCATG TGTATTCTGT CCTGCTCTGC AGTGCTGACA 
ATATATGGTA AAAAAGCAAC ACATGGAGAA TGTCAGGCCT CACAGGTACA GGATGACAGT 
GACCTGACCT TGGAAGAAGT GGCTGACCTA TGCAGAATGT ACTGTCTGAG TGTGGCCATA 
TTGCTGGTTT TGCCCCTTTC CCAGAGAATG AAATCTAGGA ACATCCATGC AGATTGGAAC 
AGCCCAGTAT TAGCATGTTT AGATTGGCAA ATAATATGTG AGCACATTAT TCTGAAGAAG 
TTTTCCAACA CCTCAAACTG GAAAATAAAT TGGACACACA TTGCTGATCT AAGTGATGGT 
TTGCTGTTGA AAAACATTGC ATATTACTAT GAAAAAGAGA ACAATACAGG CTCCAAGTTG 
ATATTCGGGA AGGCAATAGA GGAGAAGTTT TCATTTCTCT GGAACTCCAT TTTGGAAATT 
AATCCACATA GTGAACAAAT ACAATCTTAT GACGTCAGAA CAACATCACG TCCTTTCCTT 
TCAAAGGCTT CCTCAATAAT TGTGAGAGAG GAGAACATGC CAACTATAGG TCTTCTACGA 
GCAAAATCTG ACATTGTTGA AGAGTATGCT GGGGATTTTT TGGATGAATT ACCATTTTTA 
AGCAGTATTG AACATAAAGA GACCCCAGTC ACTCGAGGGA AATATTTTGA TGAACTTACA 
CACTGGCATT CTGGACGTCC ACTAAGTGAT GATTATGACA GAACAAAGGG AGATGAGGCA 
GCATTGAAAG ATGAACGTGC TCTCAGAGAT TACCAAAAGC TGCAAACTCT TCACCGTTTT 
AGCGGGCAAT CTTTAGTCTC CTCATCATCC AAGACAATTG TAATACAGAA TGATGACCCT 
GAAAAGAAGG GGGAGAAAGA AAAGGTTATT GTTAAGAAAA AGCAACCACA GAAGTCTAAA 
AAAGATCTAA TCATAGAGGA AAACCAGAAA AGAATGAATG CCAAAGAGGA AACTAAAGAC 
GCTGAACAGT GGAAATTAGT GGCATCTTCA ATTGAAAAAG AAATGAGAGC AAATTTTTTT 
CCTGGCTTAA AAAAACTGGA AAGTTTCATA AAAAATACCC AAACCCGCTC AGTGAAGCTT 
CTTGCAGAAC TATTAGGATT AAGAATTTCC TTTAAGGTGT GGGTCGAGCA CTGCTGCACA 
GACATGTCCT CAGAAGAGGA GGACGATTTA AATATTGCTG TGGAAATCAT GAAGAAGATA 
CAAACACTTC ATTATAAATA CAAGGACATG CTGACTAATG AGGATCAACA ACTAATAGCT 
CAATGTCTTA GTTATCTGGG ATTTGAGAAC TTGGCGTGCA CATTGCCGCG CGCTAAGGTA 
ACCAGTGCCA GAGAGGACAA GAAGAAGGCA GAGTGCATGG TTGGAGTTGG AGCAGCTCGT 
TTCCAATTGC AGTTTATGAG TCCATACTTG GTAAGAGAGG AGAGGACAGA TCCTGATCCA 
AGAGTACAAC ACTTTATACC GGACACATGG CAGCGTAAAC TTCTTGATGT TGTTGACAAT 
AATGAGTCTG CGGTGATAGT TGCCCCTACA TCTTCAGGAA AGACTTACGC TTCGTACTAC 
TGCATGCAGA AAGTTCTGAG TCAAGGCCAT GAAGGTGTGG TTGTGTATGT TTCACCAACA 
AAGGCTCTTG TAAACCAGGT GGTAACCACT GTAATGAATC AGTTCAACAA AAATTTGCCC 
AATGGAGTGG CTCTGTGTGG TGTTTTCACA AGGGATTACC GTACTGATGC ATTAAATTCT 
CAGATATTAG TAACAGTGCC TCAGTGTCTT GAGATTCTTC TCTTGTCGCC ACACCGTCAG 
GAATGGGCTA AAAGGATTAA GTATGTAATA TTTGATGAGA TTCATTGCCT GGGTGGAGAA 
ATAGGTGCAG AAGTATGGGA ACATCTTCTA GTGATGATTC GCTGTCCATT TCTTGCTTTG 
TCTGCAACAA TAAGTAACCC GGAACATCTA ACTGAATGGC TGCAATCAGT CAAATTTTAC 
TGGCAAAAAA GAGATAAATT AATAGAGGAA AACTGGACTG AGAAAGGAGC AAAGGCAAAA 
GCCTATAAGA AGAAAGAAAA AATGTCTTAC AGAGTTCATC TGGTGATTTA TAACAAGAGA 
TACAATGATC TGGAAAAATA TGTGTGCTCT GTGCAAGACT CCAATGTATC GTTTGAGCAC 
TATCACCCAT GTGCTGCCTT AACCACGGAC CACATGGAGA AATATGGAAT TCCAAATGAC 
CTGTCCTGCT CCCCAAGGGA GAGTTTACAA CTGTATGACA CTATGGTGGA TGTGTGGCCT 
GAATGGGCAA GGAGAGAGGA ACTCGATCCT GAAGAATACG CACATTTCAA GGACAGAATC 
ATCATAACAA AAAATGATAC AAAAATATAT GAGGAAGAAC TTAAAAAAGA ATTAATTGAC 
TGGATTAAGC AAGGAAACCA GGAAAAGGTT TCCCAACTAT TGAAGTATCT TCAGCCACCA 
AAATCATCTT GTAATGTGGA CCGTGTCGCC TACTTTCCAA AGCTTGTTGA AAAACTGAAA 
GAAGAGCAAA AGCTGCCAGC TCTTCTTTTT GCATTTAACA TTGGACTTGT GGAACATCTC 
GCACAGGAAA CAGCCATATT TCTAAAAAAC AAGCAAGACA ATAAGACAAA ACTTCTGACT 
GAGAAAAAAA GCCAAAAACT TATCACCAAA GCAAAAAGAC TGGAAAAAGC AATAGCAAAA 
AATGAACCCT CAAAAGTAAA CGACGCTTTC TTAGCCAATG TTGCCAATCA TAAGAGTATA 
GAGCAAGTAC TTATAAAGGA CAAAGAAATC CCACCAGACT GTACTTATGC TGATACCAGA 
GCTGTGAACA ATGAGTTACT CTGGGAGGTG TTCAGACAAA CAAGTCGATC AAGAGATGCC 
AAGTGTCTGA GGTTTCTGGC AGAAAGAGGT GTGGGCTACC ATCATGCCTC TTTGGATGTT 
AAAGGAAGAA GACAAGTGGA GATGCTCTTC AGGATGGGAT ACCTACGGGT TGTTACTGCA 
ACTGGATCTC TTGCCTTGGG AATCAACATG CCGTGTAAAT CTGTTGTGTT TGTTCAAGAT 
TCACTTTACC TGGATGCATT AAATTACAGA CAGATGTCAG GACGTGCAGG AAGGCGAGGA 
CAGGATGTAC TGGGAAATGT ATACTTTTTC ACCATTCCTA TGCCTAAAGT GAAACGGCTT 
ATGAAATCAG ATGTCCCTAA CTTAAAAGGA CAGTTCCCTT TAGGCATCTC TTTTGTCCTA 
CGACTGATGT TATTGGCTGC CAAAGCTGAT CACAAAGAAG ATGCCAGTGC TAAGGCTTTG 
TCAGTACTGA AGCACTCCCT GATGGCGTTC AAAAAACCTT CTGAGACCAA AATGTTGAAG 
CTCTACTTTC TGTTCTGCAT ACACTTCTTA ATCCATGAGG GCTATTTGGA TCAACATTGC 
AACCCTTTGG ACTTTACCGG GCTGGTTACA CATTTACATT ACCATGAACC ATCAAATTTT 
GTGTTTGTCA GTTTTCTGGA AAATGGTTTA TTTCACCGGC TGTGCCAGAG AAACACTAAA 
GGTTCAAATA GGTTTCCACA ATCTGTGATG GAAACCCTGG TGCTGGTATT GGCTAACTTG 
TTTGGAAGAA GCTACCTTTT ACCATCCATG CTAGAATTAA AGGGAACATT TAAACAGTCA 
AAGGTCTTTC TGGAAGGTCT ACCAGATGAT TTTGCTGCAG CTCTTGAAGA CTACAATTCC 
AAAATATCAG CAGTCTTTGG GCAGTATCTT CTTGCTGTTT CTCATCTGGC AAATTATGAA 
CAAGAATACA AGCTTCCTCT ATCACAAATC AACTTCTCAG GGGAGGAATG TGAGGATTCT 
GAACTTGTAA ACCACTTGAT GAACTGCACC AAAAGGAGAA GTGCCATAAC CCCCTTTGCC 
TGCCTGTCTG GAAATACAGA TCATGACTTA ACTTTTGCAG CACATTTGGA CAGTATTTTG 
TTGCAAACTA GTCATGTGAG TGAAAAACAC ATTCCTCGCC TGCATTTGGA GAACACAGAT 
GCTTGTGGTA GAAAACTGTT ACTAAATGCC TATGCACTGG ATTTCTTCAA GCACAGCTCC 
ATAGATGCAC TGGAAACTGA TAATGGGTTT CACAAAGGAG ATGCTTTTTA CAAGTTACGA 
GATTTTTCTT TATGCATTGC AACTATAAGA GTTTCTTTAA AGGAAATGTG TGAAGATGAG 
GATGACCCCG TTGTGTTAGC TTTCGAGCAG CTGAATACCC TGTACAGAGA GAAACTGGAG 
ACTGCTTATC CCAGGCGCAG AATCTTTTAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751318.1
CDS	126..5321
Translation	MATQGSYLGQKSLESQKEPDPESSVPCDPEPKNNAESQVKSSEFPHGMYLYVMEKLSRAVFASLLNDYVESEFFLIDGDSLLLTCVLDQSLKAGQHLHFFYLVESYLHKLTNRGAKYAVVFFEDMEFFYHDRVDLISLRTQLKLHLEHNTDTVIYTFSNFLSAQWNTFLIDHHPYFLMVCDEGVTLSQTYFFSMLMLSALGKKIDLVLTSGQEFDQLRVYGYHINATHHHNLYPIKKQKQFLQDLVDPCEKLYRKRAQKLNFSNTINEIEKSITLLFNNGDKALDIRSIMCILSCSAVLTIYGKKATHGECQASQVQDDSDLTLEEVADLCRMYCLSVAILLVLPLSQRMKSRNIHADWNSPVLACLDWQIICEHIILKKFSNTSNWKINWTHIADLSDGLLLKNIAYYYEKENNTGSKLIFGKAIEEKFSFLWNSILEINPHSEQIQSYDVRTTSRPFLSKASSIIVREENMPTIGLLRAKSDIVEEYAGDFLDELPFLSSIEHKETPVTRGKYFDELTHWHSGRPLSDDYDRTKGDEAALKDERALRDYQKLQTLHRFSGQSLVSSSSKTIVIQNDDPEKKGEKEKVIVKKKQPQKSKKDLIIEENQKRMNAKEETKDAEQWKLVASSIEKEMRANFFPGLKKLESFIKNTQTRSVKLLAELLGLRISFKVWVEHCCTDMSSEEEDDLNIAVEIMKKIQTLHYKYKDMLTNEDQQLIAQCLSYLGFENLACTLPRAKVTSAREDKKKAECMVGVGAARFQLQFMSPYLVREERTDPDPRVQHFIPDTWQRKLLDVVDNNESAVIVAPTSSGKTYASYYCMQKVLSQGHEGVVVYVSPTKALVNQVVTTVMNQFNKNLPNGVALCGVFTRDYRTDALNSQILVTVPQCLEILLLSPHRQEWAKRIKYVIFDEIHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKFYWQKRDKLIEENWTEKGAKAKAYKKKEKMSYRVHLVIYNKRYNDLEKYVCSVQDSNVSFEHYHPCAALTTDHMEKYGIPNDLSCSPRESLQLYDTMVDVWPEWARREELDPEEYAHFKDRIIITKNDTKIYEEELKKELIDWIKQGNQEKVSQLLKYLQPPKSSCNVDRVAYFPKLVEKLKEEQKLPALLFAFNIGLVEHLAQETAIFLKNKQDNKTKLLTEKKSQKLITKAKRLEKAIAKNEPSKVNDAFLANVANHKSIEQVLIKDKEIPPDCTYADTRAVNNELLWEVFRQTSRSRDAKCLRFLAERGVGYHHASLDVKGRRQVEMLFRMGYLRVVTATGSLALGINMPCKSVVFVQDSLYLDALNYRQMSGRAGRRGQDVLGNVYFFTIPMPKVKRLMKSDVPNLKGQFPLGISFVLRLMLLAAKADHKEDASAKALSVLKHSLMAFKKPSETKMLKLYFLFCIHFLIHEGYLDQHCNPLDFTGLVTHLHYHEPSNFVFVSFLENGLFHRLCQRNTKGSNRFPQSVMETLVLVLANLFGRSYLLPSMLELKGTFKQSKVFLEGLPDDFAAALEDYNSKISAVFGQYLLAVSHLANYEQEYKLPLSQINFSGEECEDSELVNHLMNCTKRRSAITPFACLSGNTDHDLTFAAHLDSILLQTSHVSEKHIPRLHLENTDACGRKLLLNAYALDFFKHSSIDALETDNGFHKGDAFYKLRDFSLCIATIRVSLKEMCEDEDDPVVLAFEQLNTLYREKLETAYPRRRIFYA

Target ORF information:

RefSeq Version	XM_031895458.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895458.1

ORF Insert Sequence:

ATGGCTACAC AAGGATCTTA TTTAGGACAG AAGTCACTGG AGTCTCAAAA AGAACCAGAT 
CCTGAATCTT CTGTACCATG TGACCCAGAA CCAAAAAACA ATGCTGAATC CCAGGTGAAA 
AGTTCTGAAT TCCCCCATGG CATGTACTTA TATGTAATGG AGAAACTCTC AAGAGCTGTC 
TTTGCCAGCC TGCTTAATGA TTATGTGGAA TCTGAATTCT TCTTGATTGA TGGAGATTCC 
TTACTGCTAA CTTGTGTTTT GGATCAGTCA CTGAAGGCAG GGCAGCATCT GCATTTCTTT 
TACTTGGTAG AATCCTACTT GCATAAACTG ACCAACCGGG GAGCAAAATA TGCTGTTGTG 
TTTTTTGAGG ATATGGAGTT TTTCTACCAT GACAGAGTCG ACCTTATCTC TCTACGGACC 
CAACTGAAAC TGCACTTGGA ACACAACACA GACACAGTCA TATACACCTT TTCCAACTTT 
TTGTCTGCTC AGTGGAACAC ATTTCTGATT GATCACCATC CCTACTTTCT AATGGTTTGT 
GATGAAGGTG TTACCCTGTC TCAAACATAT TTCTTCAGTA TGCTTATGCT AAGTGCCCTG 
GGGAAAAAAA TAGACCTCGT ACTCACGTCA GGACAAGAGT TCGATCAGCT CCGAGTGTAT 
GGATACCATA TCAATGCCAC CCACCATCAT AACTTATATC CCATAAAGAA ACAGAAGCAG 
TTTCTTCAAG ATTTGGTAGA TCCATGTGAA AAGTTGTACA GAAAGCGTGC ACAGAAGCTG 
AATTTCAGTA ACACCATAAA CGAGATTGAA AAGAGTATAA CATTACTTTT TAACAATGGG 
GATAAGGCTT TAGACATAAG AAGCATCATG TGTATTCTGT CCTGCTCTGC AGTGCTGACA 
ATATATGGTA AAAAAGCAAC ACATGGAGAA TGTCAGGCCT CACAGGTACA GGATGACAGT 
GACCTGACCT TGGAAGAAGT GGCTGACCTA TGCAGAATGT ACTGTCTGAG TGTGGCCATA 
TTGCTGGTTT TGCCCCTTTC CCAGAGAATG AAATCTAGGA ACATCCATGC AGATTGGAAC 
AGCCCAGTAT TAGCATGTTT AGATTGGCAA ATAATATGTG AGCACATTAT TCTGAAGAAG 
TTTTCCAACA CCTCAAACTG GAAAATAAAT TGGACACACA TTGCTGATCT AAGTGATGGT 
TTGCTGTTGA AAAACATTGC ATATTACTAT GAAAAAGAGA ACAATACAGG CTCCAAGTTG 
ATATTCGGGA AGGCAATAGA GGAGAAGTTT TCATTTCTCT GGAACTCCAT TTTGGAAATT 
AATCCACATA GTGAACAAAT ACAATCTTAT GACGTCAGAA CAACATCACG TCCTTTCCTT 
TCAAAGGCTT CCTCAATAAT TGTGAGAGAG GAGAACATGC CAACTATAGG TCTTCTACGA 
GCAAAATCTG ACATTGTTGA AGAGTATGCT GGGGATTTTT TGGATGAATT ACCATTTTTA 
AGCAGTATTG AACATAAAGA GACCCCAGTC ACTCGAGGGA AATATTTTGA TGAACTTACA 
CACTGGCATT CTGGACGTCC ACTAAGTGAT GATTATGACA GAACAAAGGG AGATGAGGCA 
GCATTGAAAG ATGAACGTGC TCTCAGAGAT TACCAAAAGC TGCAAACTCT TCACCGTTTT 
AGCGGGCAAT CTTTAGTCTC CTCATCATCC AAGACAATTG TAATACAGAA TGATGACCCT 
GAAAAGAAGG GGGAGAAAGA AAAGGTTATT GTTAAGAAAA AGCAACCACA GAAGTCTAAA 
AAAGATCTAA TCATAGAGGA AAACCAGAAA AGAATGAATG CCAAAGAGGA AACTAAAGAC 
GCTGAACAGT GGAAATTAGT GGCATCTTCA ATTGAAAAAG AAATGAGAGC AAATTTTTTT 
CCTGGCTTAA AAAAACTGGA AAGTTTCATA AAAAATACCC AAACCCGCTC AGTGAAGCTT 
CTTGCAGAAC TATTAGGATT AAGAATTTCC TTTAAGGTGT GGGTCGAGCA CTGCTGCACA 
GACATGTCCT CAGAAGAGGA GGACGATTTA AATATTGCTG TGGAAATCAT GAAGAAGATA 
CAAACACTTC ATTATAAATA CAAGGACATG CTGACTAATG AGGATCAACA ACTAATAGCT 
CAATGTCTTA GTTATCTGGG ATTTGAGAAC TTGGCGTGCA CATTGCCGCG CGCTAAGGTA 
ACCAGTGCCA GAGAGGACAA GAAGAAGGCA GAGTGCATGG TTGGAGTTGG AGCAGCTCGT 
TTCCAATTGC AGTTTATGAG TCCATACTTG GTAAGAGAGG AGAGGACAGA TCCTGATCCA 
AGAGTACAAC ACTTTATACC GGACACATGG CAGCGTAAAC TTCTTGATGT TGTTGACAAT 
AATGAGTCTG CGGTGATAGT TGCCCCTACA TCTTCAGGAA AGACTTACGC TTCGTACTAC 
TGCATGCAGA AAGTTCTGAG TCAAGGCCAT GAAGGTGTGG TTGTGTATGT TTCACCAACA 
AAGGCTCTTG TAAACCAGGT GGTAACCACT GTAATGAATC AGTTCAACAA AAATTTGCCC 
AATGGAGTGG CTCTGTGTGG TGTTTTCACA AGGGATTACC GTACTGATGC ATTAAATTCT 
CAGATATTAG TAACAGTGCC TCAGTGTCTT GAGATTCTTC TCTTGTCGCC ACACCGTCAG 
GAATGGGCTA AAAGGATTAA GTATGTAATA TTTGATGAGA TTCATTGCCT GGGTGGAGAA 
ATAGGTGCAG AAGTATGGGA ACATCTTCTA GTGATGATTC GCTGTCCATT TCTTGCTTTG 
TCTGCAACAA TAAGTAACCC GGAACATCTA ACTGAATGGC TGCAATCAGT CAAATTTTAC 
TGGCAAAAAA GAGATAAATT AATAGAGGAA AACTGGACTG AGAAAGGAGC AAAGGCAAAA 
GCCTATAAGA AGAAAGAAAA AATGTCTTAC AGAGTTCATC TGGTGATTTA TAACAAGAGA 
TACAATGATC TGGAAAAATA TGTGTGCTCT GTGCAAGACT CCAATGTATC GTTTGAGCAC 
TATCACCCAT GTGCTGCCTT AACCACGGAC CACATGGAGA AATATGGAAT TCCAAATGAC 
CTGTCCTGCT CCCCAAGGGA GAGTTTACAA CTGTATGACA CTATGGTGGA TGTGTGGCCT 
GAATGGGCAA GGAGAGAGGA ACTCGATCCT GAAGAATACG CACATTTCAA GGACAGAATC 
ATCATAACAA AAAATGATAC AAAAATATAT GAGGAAGAAC TTAAAAAAGA ATTAATTGAC 
TGGATTAAGC AAGGAAACCA GGAAAAGGTT TCCCAACTAT TGAAGTATCT TCAGCCACCA 
AAATCATCTT GTAATGTGGA CCGTGTCGCC TACTTTCCAA AGCTTGTTGA AAAACTGAAA 
GAAGAGCAAA AGCTGCCAGC TCTTCTTTTT GCATTTAACA TTGGACTTGT GGAACATCTC 
GCACAGGAAA CAGCCATATT TCTAAAAAAC AAGCAAGACA ATAAGACAAA ACTTCTGACT 
GAGAAAAAAA GCCAAAAACT TATCACCAAA GCAAAAAGAC TGGAAAAAGC AATAGCAAAA 
AATGAACCCT CAAAAGTAAA CGACGCTTTC TTAGCCAATG TTGCCAATCA TAAGAGTATA 
GAGCAAGTAC TTATAAAGGA CAAAGAAATC CCACCAGACT GTACTTATGC TGATACCAGA 
GCTGTGAACA ATGAGTTACT CTGGGAGGTG TTCAGACAAA CAAGTCGATC AAGAGATGCC 
AAGTGTCTGA GGTTTCTGGC AGAAAGAGGT GTGGGCTACC ATCATGCCTC TTTGGATGTT 
AAAGGAAGAA GACAAGTGGA GATGCTCTTC AGGATGGGAT ACCTACGGGT TGTTACTGCA 
ACTGGATCTC TTGCCTTGGG AATCAACATG CCGTGTAAAT CTGTTGTGTT TGTTCAAGAT 
TCACTTTACC TGGATGCATT AAATTACAGA CAGATGTCAG GACGTGCAGG AAGGCGAGGA 
CAGGATGTAC TGGGAAATGT ATACTTTTTC ACCATTCCTA TGCCTAAAGT GAAACGGCTT 
ATGAAATCAG ATGTCCCTAA CTTAAAAGGA CAGTTCCCTT TAGGCATCTC TTTTGTCCTA 
CGACTGATGT TATTGGCTGC CAAAGCTGAT CACAAAGAAG ATGCCAGTGC TAAGGCTTTG 
TCAGTACTGA AGCACTCCCT GATGGCGTTC AAAAAACCTT CTGAGACCAA AATGTTGAAG 
CTCTACTTTC TGTTCTGCAT ACACTTCTTA ATCCATGAGG GCTATTTGGA TCAACATTGC 
AACCCTTTGG ACTTTACCGG GCTGGTTACA CATTTACATT ACCATGAACC ATCAAATTTT 
GTGTTTGTCA GTTTTCTGGA AAATGGTTTA TTTCACCGGC TGTGCCAGAG AAACACTAAA 
GGTTCAAATA GGTTTCCACA ATCTGTGATG GAAACCCTGG TGCTGGTATT GGCTAACTTG 
TTTGGAAGAA GCTACCTTTT ACCATCCATG CTAGAATTAA AGGGAACATT TAAACAGTCA 
AAGGTCTTTC TGGAAGGTCT ACCAGATGAT TTTGCTGCAG CTCTTGAAGA CTACAATTCC 
AAAATATCAG CAGTCTTTGG GCAGTATCTT CTTGCTGTTT CTCATCTGGC AAATTATGAA 
CAAGAATACA AGCTTCCTCT ATCACAAATC AACTTCTCAG GGGAGGAATG TGAGGATTCT 
GAACTTGTAA ACCACTTGAT GAACTGCACC AAAAGGAGAA GTGCCATAAC CCCCTTTGCC 
TGCCTGTCTG GAAATACAGA TCATGACTTA ACTTTTGCAG CACATTTGGA CAGTATTTTG 
TTGCAAACTA GTCATGTGAG TGAAAAACAC ATTCCTCGCC TGCATTTGGA GAACACAGAT 
GCTTGTGGTA GAAAACTGTT ACTAAATGCC TATGCACTGG ATTTCTTCAA GCACAGCTCC 
ATAGATGCAC TGGAAACTGA TAATGGGTTT CACAAAGGAG ATGCTTTTTA CAAGTTACGA 
GATTTTTCTT TATGCATTGC AACTATAAGA GTTTCTTTAA AGGAAATGTG TGAAGATGAG 
GATGACCCCG TTGTGTTAGC TTTCGAGCAG CTGAATACCC TGTACAGAGA GAAACTGGAG 
ACTGCTTATC CCAGGCGCAG AATCTTTTAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx60 ( XM_031895459.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751319.1 Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa49815
Clone ID Related Accession (Same CDS sequence)	XM_031895459.1
Accession Version	XM_031895459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX60 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAC AAGGATCTTA TTTAGGACAG AAGTCACTGG AGTCTCAAAA AGAACCAGAT 
CCTGAATCTT CTGTACCATG TGACCCAGAA CCAAAAAACA ATGCTGAATC CCAGGTGAAA 
AGTTCTGAAT TCCCCCATGG CATGTACTTA TATGTAATGG AGAAACTCTC AAGAGCTGTC 
TTTGCCAGCC TGCTCAATGA TTATGTGGAA TCTGAATTCT TCTTGATTGA TGGAGATTCC 
TTACAGCTAA CTTGTGTTTT GGATCAGTCA CTGAAGGCAG GGCAGCATCT GCATTTCTTC 
TACTTGGTAG AATCCTACTT GTATAAACTG ACCAACCGGG GAGCAAAATA TGCTGTTGTG 
TTTTTTGAGG ATATGGAGTT TTTCTACCAT GACAGAGTCG ACCTTATCTC TCTACGGACC 
CAACTGAAAC TGCACTTGGA ACACAACACA GACACAGTCA TATACACCTT TTCCAACTTT 
TTGTCTGCTC AGTGGAACAC ATTTCTGATT GATCACCATC CCTACTTTCT AATGGTTTGT 
GATGAAGGTG TTACCCTGTC TCAAACATAT TTCTTCAGTA TGCTTATGCT AAGTGCCCTG 
GGGAAAAAAA TAGACCTCGT ACTCACGTCA GGACAAGAGT TCGATCAGCT CCGAGTGTAT 
GGATACCATA TCAATGCCAC CCACCATCAT AACTTATATC CCATAAAGAA ACAGAAGCAG 
TTTCTTCAAG ATTTGGTAGA TCCATGTGAA AAGTTGTACA GAAAGCGTGC ACAGAAGCTG 
AATTTCAGTA ACACCATAAA CGAGATTGAA AAGAGTATAA CATTACTTTT TAACAATGGG 
GATAAGGCTT TAGACATAAG AAGCATCATG TGTATTCTGT CCTGCTCTGC AGTGCTGACA 
ATATATGGTA AAAAAGCAAC ACATGGAGAA TGTCAGGCCT CACAGGTACA GGATGACAGT 
GACCTGACCT TGGAAGAAGT GGCTGACCTA TGCAGAATGT ACTGTCTGAG TGTGGCCATA 
TTGCTGGTTT TGCCCCTTTC CCAGAGAATG AAATCTAGGA ACATCCATGC AGATTGGAAC 
AGCCCAGTAT TAGCATGTTT AGATTGGCAA ATAATATGTG AGCACATTAT TCTGAAGAAG 
TTTTCCAACA CCTCAAACTG GAAAATAAAT TGGACACACA TTGCTGATCT AAGTGATGGT 
TTGCTGTTGA AAAACATTGC ATATTACTAT GAAAAAGAGA ACAATACAGG CTCCAAGTTG 
ATATTCGGGA AGGCAATAGA GGAGAAGTTT TCATTTCTCT GGAACTCCAT TTTGGAAATT 
AATCCACATA GTGAACAAAT ACAATCTTAT GACGTCAGAA CAACATCACG TCCTTTCCTT 
TCAAAGGCTT CCTCAATAAT TGTGAGAGAG GAGAACATGC CAACTATAGG TCTTCTACGA 
GCAAAATCTG ACATTGTTGA AGAGTATGCT GGGGATTTTT TGGATGAATT ACCATTTTTA 
AGCAGTATTG AACATAAAGA GACCCCAGTC ACTCGAGGGA AATATTTTGA TGAACTTACA 
CACTGGCATT CTGGACGTCC ACTAAGTGAT GATTATGACA GAACAAAGGG AGATGAGGCA 
GCATTGAAAG ATGAACGTGC TCTCAGAGAT TACCAAAAGC TGCAAACTCT TCACCGTTTT 
AGCGGGCAAT CTTTAGTCTC CTCATCATCC AAGACAATTG TAATACAGAA TGATGACCCT 
GAAAAGAAGG GGGAGAAAGA AAAGGTTATT GTTAAGAAAA AGCAACCACA GAAGTCTAAA 
AAAGATCTAA TCATAGAGGA AAACCAGAAA AGAATGAATG CCAAAGAGGA AACTAAAGAC 
GCTGAACAGT GGAAATTAGT GGCATCTTCA ATTGAAAAAG AAATGAGAGC AAATTTTTTT 
CCTGGCTTAA AAAAACTGGA AAGTTTCATA AAAAATACCC AAACCCGCTC AGTGAAGCTT 
CTTGCAGAAC TATTAGGATT AAGAATTTCC TTTAAGGTGT GGGTCGAGCA CTGCTGCACA 
GACATGTCCT CAGAAGAGGA GGACGATTTA AATATTGCTG TGGAAATCAT GAAGAAGATA 
CAAACACTTC ATTATAAATA CAAGGACATG CTGACTAATG AGGATCAACA ACTAATAGCT 
CAATGTCTTA GTTATCTGGG ATTTGAGAAC TTGGCGTGCA CATTGCCGCG CGCTAAGGTA 
ACCAGTGCCA GAGAGGACAA GAAGAAGGCA GAGTGCATGG TTGGAGTTGG AGCAGCTCGT 
TTCCAATTGC AGTTTATGAG TCCATACTTG GTAAGAGAGG AGAGGACAGA TCCTGATCCA 
AGAGTACAAC ACTTTATACC GGACACATGG CAGCGTAAAC TTCTTGATGT TGTTGACAAT 
AATGAGTCTG CGGTGATAGT TGCCCCTACA TCTTCAGGAA AGACTTACGC TTCGTACTAC 
TGCATGCAGA AAGTTCTGAG TCAAGGCCAT GAAGGTGTGG TTGTGTATGT TTCACCAACA 
AAGGCTCTTG TAAACCAGGT GGTAACCACT GTAATGAATC AGTTCAACAA AAATTTGCCC 
AATGGAGTGG CTCTGTGTGG TGTTTTCACA AGGGATTACC GTACTGATGC ATTAAATTCT 
CAGATATTAG TAACAGTGCC TCAGTGTCTT GAGATTCTTC TCTTGTCGCC ACACCGTCAG 
GAATGGGCTA AAAGGATTAA GTATGTAATA TTTGATGAGA TTCATTGCCT GGGTGGAGAA 
ATAGGTGCAG AAGTATGGGA ACATCTTCTA GTGATGATTC GCTGTCCATT TCTTGCTTTG 
TCTGCAACAA TAAGTAACCC GGAACATCTA ACTGAATGGC TGCAATCAGT CAAATTTTAC 
TGGCAAAAAA GAGATAAATT AATAGAGGAA AACTGGACTG AGAAAGGAGC AAAGGCAAAA 
GCCTATAAGA AGAAAGAAAA AATGTCTTAC AGAGTTCATC TGGTGATTTA TAACAAGAGA 
TACAATGATC TGGAAAAATA TGTGTGCTCT GTGCAAGACT CCAATGTATC GTTTGAGCAC 
TATCACCCAT GTGCTGCCTT AACCACGGAC CACATGGAGA AATATGGAAT TCCAAATGAC 
CTGTCCTGCT CCCCAAGGGA GAGTTTACAA CTGTATGACA CTATGGTGGA TGTGTGGCCT 
GAATGGGCAA GGAGAGAGGA ACTCGATCCT GAAGAATACG CACATTTCAA GGACAGAATC 
ATCATAACAA AAAATGATAC AAAAATATAT GAGGAAGAAC TTAAAAAAGA ATTAATTGAC 
TGGATTAAGC AAGGAAACCA GGAAAAGGTT TCCCAACTAT TGAAGTATCT TCAGCCACCA 
AAATCATCTT GTAATGTGGA CCGTGTCGCC TACTTTCCAA AGCTTGTTGA AAAACTGAAA 
GAAGAGCAAA AGCTGCCAGC TCTTCTTTTT GCATTTAACA TTGGACTTGT GGAACATCTC 
GCACAGGAAA CAGCCATATT TCTAAAAAAC AAGCAAGACA ATAAGACAAA ACTTCTGACT 
GAGAAAAAAA GCCAAAAACT TATCACCAAA GCAAAAAGAC TGGAAAAAGC AATAGCAAAA 
AATGAACCCT CAAAAGTAAA CGACGCTTTC TTAGCCAATG TTGCCAATCA TAAGAGTATA 
GAGCAAGTAC TTATAAAGGA CAAAGAAATC CCACCAGACT GTACTTATGC TGATACCAGA 
GCTGTGAACA ATGAGTTACT CTGGGAGGTG TTCAGACAAA CAAGTCGATC AAGAGATGCC 
AAGTGTCTGA GGTTTCTGGC AGAAAGAGGT GTGGGCTACC ATCATGCCTC TTTGGATGTT 
AAAGGAAGAA GACAAGTGGA GATGCTCTTC AGGATGGGAT ACCTACGGGT TGTTACTGCA 
ACTGGATCTC TTGCCTTGGG AATCAACATG CCGTGTAAAT CTGTTGTGTT TGTTCAAGAT 
TCACTTTACC TGGATGCATT AAATTACAGA CAGATGTCAG GACGTGCAGG AAGGCGAGGA 
CAGGATGTAC TGGGAAATGT ATACTTTTTC ACCATTCCTA TGCCTAAAGT GAAACGGCTT 
ATGAAATCAG ATGTCCCTAA CTTAAAAGGA CAGTTCCCTT TAGGCATCTC TTTTGTCCTA 
CGACTGATGT TATTGGCTGC CAAAGCTGAT CACAAAGAAG ATGCCAGTGC TAAGGCTTTG 
TCAGTACTGA AGCACTCCCT GATGGCGTTC AAAAAACCTT CTGAGACCAA AATGTTGAAG 
CTCTACTTTC TGTTCTGCAT ACACTTCTTA ATCCATGAGG GCTATTTGGA TCAACATTGC 
AACCCTTTGG ACTTTACCGG GCTGGTTACA CATTTACATT ACCATGAACC ATCAAATTTT 
GTGTTTGTCA GTTTTCTGGA AAATGGTTTA TTTCACCGGC TGTGCCAGAG AAACACTAAA 
GGTTCAAATA GGTTTCCACA ATCTGTGATG GAAACCCTGG TGCTGGTATT GGCTAACTTG 
TTTGGAAGAA GCTACCTTTT ACCATCCATG CTAGAATTAA AGGGAACATT TAAACAGTCA 
AAGGTCTTTC TGGAAGGTCT ACCAGATGAT TTTGCTGCAG CTCTTGAAGA CTACAATTCC 
AAAATATCAG CAGTCTTTGG GCAGTATCTT CTTGCTGTTT CTCATCTGGC AAATTATGAA 
CAAGAATACA AGCTTCCTCT ATCACAAATC AACTTCTCAG GGGAGGAATG TGAGGATTCT 
GAACTTGTAA ACCACTTGAT GAACTGCACC AAAAGGAGAA GTGCCATAAC CCCCTTTGCC 
TGCCTGTCTG GAAATACAGA TCATGACTTA ACTTTTGCAG CACATTTGGA CAGTATTTTG 
TTGCAAACTA GTCATGTGAG TGAAAAACAC ATTCCTCGCC TGCATTTGGA GAACACAGAT 
GCTTGTGGTA GAAAACTGTT ACTAAATGCC TATGCACTGG ATTTCTTCAA GCACAGCTCC 
ATAGATGCAC TGGAAACTGA TAATGGGTTT CACAAAGGAG ATGCTTTTTA CAAGTTACGA 
GATTTTTCTT TATGCATTGC AACTATAAGA GTTTCTTTAA AGGAAATGTG TGAAGATGAG 
GATGACCCCG TTGTGTTAGC TTTCGAGCAG CTGAATACCC TGTACAGAGA GAAACTGGAG 
ACTGCTTATC CCAGGCGCAG AATCTTTTAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751319.1
CDS	126..5321
Translation	MATQGSYLGQKSLESQKEPDPESSVPCDPEPKNNAESQVKSSEFPHGMYLYVMEKLSRAVFASLLNDYVESEFFLIDGDSLQLTCVLDQSLKAGQHLHFFYLVESYLYKLTNRGAKYAVVFFEDMEFFYHDRVDLISLRTQLKLHLEHNTDTVIYTFSNFLSAQWNTFLIDHHPYFLMVCDEGVTLSQTYFFSMLMLSALGKKIDLVLTSGQEFDQLRVYGYHINATHHHNLYPIKKQKQFLQDLVDPCEKLYRKRAQKLNFSNTINEIEKSITLLFNNGDKALDIRSIMCILSCSAVLTIYGKKATHGECQASQVQDDSDLTLEEVADLCRMYCLSVAILLVLPLSQRMKSRNIHADWNSPVLACLDWQIICEHIILKKFSNTSNWKINWTHIADLSDGLLLKNIAYYYEKENNTGSKLIFGKAIEEKFSFLWNSILEINPHSEQIQSYDVRTTSRPFLSKASSIIVREENMPTIGLLRAKSDIVEEYAGDFLDELPFLSSIEHKETPVTRGKYFDELTHWHSGRPLSDDYDRTKGDEAALKDERALRDYQKLQTLHRFSGQSLVSSSSKTIVIQNDDPEKKGEKEKVIVKKKQPQKSKKDLIIEENQKRMNAKEETKDAEQWKLVASSIEKEMRANFFPGLKKLESFIKNTQTRSVKLLAELLGLRISFKVWVEHCCTDMSSEEEDDLNIAVEIMKKIQTLHYKYKDMLTNEDQQLIAQCLSYLGFENLACTLPRAKVTSAREDKKKAECMVGVGAARFQLQFMSPYLVREERTDPDPRVQHFIPDTWQRKLLDVVDNNESAVIVAPTSSGKTYASYYCMQKVLSQGHEGVVVYVSPTKALVNQVVTTVMNQFNKNLPNGVALCGVFTRDYRTDALNSQILVTVPQCLEILLLSPHRQEWAKRIKYVIFDEIHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKFYWQKRDKLIEENWTEKGAKAKAYKKKEKMSYRVHLVIYNKRYNDLEKYVCSVQDSNVSFEHYHPCAALTTDHMEKYGIPNDLSCSPRESLQLYDTMVDVWPEWARREELDPEEYAHFKDRIIITKNDTKIYEEELKKELIDWIKQGNQEKVSQLLKYLQPPKSSCNVDRVAYFPKLVEKLKEEQKLPALLFAFNIGLVEHLAQETAIFLKNKQDNKTKLLTEKKSQKLITKAKRLEKAIAKNEPSKVNDAFLANVANHKSIEQVLIKDKEIPPDCTYADTRAVNNELLWEVFRQTSRSRDAKCLRFLAERGVGYHHASLDVKGRRQVEMLFRMGYLRVVTATGSLALGINMPCKSVVFVQDSLYLDALNYRQMSGRAGRRGQDVLGNVYFFTIPMPKVKRLMKSDVPNLKGQFPLGISFVLRLMLLAAKADHKEDASAKALSVLKHSLMAFKKPSETKMLKLYFLFCIHFLIHEGYLDQHCNPLDFTGLVTHLHYHEPSNFVFVSFLENGLFHRLCQRNTKGSNRFPQSVMETLVLVLANLFGRSYLLPSMLELKGTFKQSKVFLEGLPDDFAAALEDYNSKISAVFGQYLLAVSHLANYEQEYKLPLSQINFSGEECEDSELVNHLMNCTKRRSAITPFACLSGNTDHDLTFAAHLDSILLQTSHVSEKHIPRLHLENTDACGRKLLLNAYALDFFKHSSIDALETDNGFHKGDAFYKLRDFSLCIATIRVSLKEMCEDEDDPVVLAFEQLNTLYREKLETAYPRRRIFYA

Target ORF information:

RefSeq Version	XM_031895459.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895459.1

ORF Insert Sequence:

ATGGCTACAC AAGGATCTTA TTTAGGACAG AAGTCACTGG AGTCTCAAAA AGAACCAGAT 
CCTGAATCTT CTGTACCATG TGACCCAGAA CCAAAAAACA ATGCTGAATC CCAGGTGAAA 
AGTTCTGAAT TCCCCCATGG CATGTACTTA TATGTAATGG AGAAACTCTC AAGAGCTGTC 
TTTGCCAGCC TGCTCAATGA TTATGTGGAA TCTGAATTCT TCTTGATTGA TGGAGATTCC 
TTACAGCTAA CTTGTGTTTT GGATCAGTCA CTGAAGGCAG GGCAGCATCT GCATTTCTTC 
TACTTGGTAG AATCCTACTT GTATAAACTG ACCAACCGGG GAGCAAAATA TGCTGTTGTG 
TTTTTTGAGG ATATGGAGTT TTTCTACCAT GACAGAGTCG ACCTTATCTC TCTACGGACC 
CAACTGAAAC TGCACTTGGA ACACAACACA GACACAGTCA TATACACCTT TTCCAACTTT 
TTGTCTGCTC AGTGGAACAC ATTTCTGATT GATCACCATC CCTACTTTCT AATGGTTTGT 
GATGAAGGTG TTACCCTGTC TCAAACATAT TTCTTCAGTA TGCTTATGCT AAGTGCCCTG 
GGGAAAAAAA TAGACCTCGT ACTCACGTCA GGACAAGAGT TCGATCAGCT CCGAGTGTAT 
GGATACCATA TCAATGCCAC CCACCATCAT AACTTATATC CCATAAAGAA ACAGAAGCAG 
TTTCTTCAAG ATTTGGTAGA TCCATGTGAA AAGTTGTACA GAAAGCGTGC ACAGAAGCTG 
AATTTCAGTA ACACCATAAA CGAGATTGAA AAGAGTATAA CATTACTTTT TAACAATGGG 
GATAAGGCTT TAGACATAAG AAGCATCATG TGTATTCTGT CCTGCTCTGC AGTGCTGACA 
ATATATGGTA AAAAAGCAAC ACATGGAGAA TGTCAGGCCT CACAGGTACA GGATGACAGT 
GACCTGACCT TGGAAGAAGT GGCTGACCTA TGCAGAATGT ACTGTCTGAG TGTGGCCATA 
TTGCTGGTTT TGCCCCTTTC CCAGAGAATG AAATCTAGGA ACATCCATGC AGATTGGAAC 
AGCCCAGTAT TAGCATGTTT AGATTGGCAA ATAATATGTG AGCACATTAT TCTGAAGAAG 
TTTTCCAACA CCTCAAACTG GAAAATAAAT TGGACACACA TTGCTGATCT AAGTGATGGT 
TTGCTGTTGA AAAACATTGC ATATTACTAT GAAAAAGAGA ACAATACAGG CTCCAAGTTG 
ATATTCGGGA AGGCAATAGA GGAGAAGTTT TCATTTCTCT GGAACTCCAT TTTGGAAATT 
AATCCACATA GTGAACAAAT ACAATCTTAT GACGTCAGAA CAACATCACG TCCTTTCCTT 
TCAAAGGCTT CCTCAATAAT TGTGAGAGAG GAGAACATGC CAACTATAGG TCTTCTACGA 
GCAAAATCTG ACATTGTTGA AGAGTATGCT GGGGATTTTT TGGATGAATT ACCATTTTTA 
AGCAGTATTG AACATAAAGA GACCCCAGTC ACTCGAGGGA AATATTTTGA TGAACTTACA 
CACTGGCATT CTGGACGTCC ACTAAGTGAT GATTATGACA GAACAAAGGG AGATGAGGCA 
GCATTGAAAG ATGAACGTGC TCTCAGAGAT TACCAAAAGC TGCAAACTCT TCACCGTTTT 
AGCGGGCAAT CTTTAGTCTC CTCATCATCC AAGACAATTG TAATACAGAA TGATGACCCT 
GAAAAGAAGG GGGAGAAAGA AAAGGTTATT GTTAAGAAAA AGCAACCACA GAAGTCTAAA 
AAAGATCTAA TCATAGAGGA AAACCAGAAA AGAATGAATG CCAAAGAGGA AACTAAAGAC 
GCTGAACAGT GGAAATTAGT GGCATCTTCA ATTGAAAAAG AAATGAGAGC AAATTTTTTT 
CCTGGCTTAA AAAAACTGGA AAGTTTCATA AAAAATACCC AAACCCGCTC AGTGAAGCTT 
CTTGCAGAAC TATTAGGATT AAGAATTTCC TTTAAGGTGT GGGTCGAGCA CTGCTGCACA 
GACATGTCCT CAGAAGAGGA GGACGATTTA AATATTGCTG TGGAAATCAT GAAGAAGATA 
CAAACACTTC ATTATAAATA CAAGGACATG CTGACTAATG AGGATCAACA ACTAATAGCT 
CAATGTCTTA GTTATCTGGG ATTTGAGAAC TTGGCGTGCA CATTGCCGCG CGCTAAGGTA 
ACCAGTGCCA GAGAGGACAA GAAGAAGGCA GAGTGCATGG TTGGAGTTGG AGCAGCTCGT 
TTCCAATTGC AGTTTATGAG TCCATACTTG GTAAGAGAGG AGAGGACAGA TCCTGATCCA 
AGAGTACAAC ACTTTATACC GGACACATGG CAGCGTAAAC TTCTTGATGT TGTTGACAAT 
AATGAGTCTG CGGTGATAGT TGCCCCTACA TCTTCAGGAA AGACTTACGC TTCGTACTAC 
TGCATGCAGA AAGTTCTGAG TCAAGGCCAT GAAGGTGTGG TTGTGTATGT TTCACCAACA 
AAGGCTCTTG TAAACCAGGT GGTAACCACT GTAATGAATC AGTTCAACAA AAATTTGCCC 
AATGGAGTGG CTCTGTGTGG TGTTTTCACA AGGGATTACC GTACTGATGC ATTAAATTCT 
CAGATATTAG TAACAGTGCC TCAGTGTCTT GAGATTCTTC TCTTGTCGCC ACACCGTCAG 
GAATGGGCTA AAAGGATTAA GTATGTAATA TTTGATGAGA TTCATTGCCT GGGTGGAGAA 
ATAGGTGCAG AAGTATGGGA ACATCTTCTA GTGATGATTC GCTGTCCATT TCTTGCTTTG 
TCTGCAACAA TAAGTAACCC GGAACATCTA ACTGAATGGC TGCAATCAGT CAAATTTTAC 
TGGCAAAAAA GAGATAAATT AATAGAGGAA AACTGGACTG AGAAAGGAGC AAAGGCAAAA 
GCCTATAAGA AGAAAGAAAA AATGTCTTAC AGAGTTCATC TGGTGATTTA TAACAAGAGA 
TACAATGATC TGGAAAAATA TGTGTGCTCT GTGCAAGACT CCAATGTATC GTTTGAGCAC 
TATCACCCAT GTGCTGCCTT AACCACGGAC CACATGGAGA AATATGGAAT TCCAAATGAC 
CTGTCCTGCT CCCCAAGGGA GAGTTTACAA CTGTATGACA CTATGGTGGA TGTGTGGCCT 
GAATGGGCAA GGAGAGAGGA ACTCGATCCT GAAGAATACG CACATTTCAA GGACAGAATC 
ATCATAACAA AAAATGATAC AAAAATATAT GAGGAAGAAC TTAAAAAAGA ATTAATTGAC 
TGGATTAAGC AAGGAAACCA GGAAAAGGTT TCCCAACTAT TGAAGTATCT TCAGCCACCA 
AAATCATCTT GTAATGTGGA CCGTGTCGCC TACTTTCCAA AGCTTGTTGA AAAACTGAAA 
GAAGAGCAAA AGCTGCCAGC TCTTCTTTTT GCATTTAACA TTGGACTTGT GGAACATCTC 
GCACAGGAAA CAGCCATATT TCTAAAAAAC AAGCAAGACA ATAAGACAAA ACTTCTGACT 
GAGAAAAAAA GCCAAAAACT TATCACCAAA GCAAAAAGAC TGGAAAAAGC AATAGCAAAA 
AATGAACCCT CAAAAGTAAA CGACGCTTTC TTAGCCAATG TTGCCAATCA TAAGAGTATA 
GAGCAAGTAC TTATAAAGGA CAAAGAAATC CCACCAGACT GTACTTATGC TGATACCAGA 
GCTGTGAACA ATGAGTTACT CTGGGAGGTG TTCAGACAAA CAAGTCGATC AAGAGATGCC 
AAGTGTCTGA GGTTTCTGGC AGAAAGAGGT GTGGGCTACC ATCATGCCTC TTTGGATGTT 
AAAGGAAGAA GACAAGTGGA GATGCTCTTC AGGATGGGAT ACCTACGGGT TGTTACTGCA 
ACTGGATCTC TTGCCTTGGG AATCAACATG CCGTGTAAAT CTGTTGTGTT TGTTCAAGAT 
TCACTTTACC TGGATGCATT AAATTACAGA CAGATGTCAG GACGTGCAGG AAGGCGAGGA 
CAGGATGTAC TGGGAAATGT ATACTTTTTC ACCATTCCTA TGCCTAAAGT GAAACGGCTT 
ATGAAATCAG ATGTCCCTAA CTTAAAAGGA CAGTTCCCTT TAGGCATCTC TTTTGTCCTA 
CGACTGATGT TATTGGCTGC CAAAGCTGAT CACAAAGAAG ATGCCAGTGC TAAGGCTTTG 
TCAGTACTGA AGCACTCCCT GATGGCGTTC AAAAAACCTT CTGAGACCAA AATGTTGAAG 
CTCTACTTTC TGTTCTGCAT ACACTTCTTA ATCCATGAGG GCTATTTGGA TCAACATTGC 
AACCCTTTGG ACTTTACCGG GCTGGTTACA CATTTACATT ACCATGAACC ATCAAATTTT 
GTGTTTGTCA GTTTTCTGGA AAATGGTTTA TTTCACCGGC TGTGCCAGAG AAACACTAAA 
GGTTCAAATA GGTTTCCACA ATCTGTGATG GAAACCCTGG TGCTGGTATT GGCTAACTTG 
TTTGGAAGAA GCTACCTTTT ACCATCCATG CTAGAATTAA AGGGAACATT TAAACAGTCA 
AAGGTCTTTC TGGAAGGTCT ACCAGATGAT TTTGCTGCAG CTCTTGAAGA CTACAATTCC 
AAAATATCAG CAGTCTTTGG GCAGTATCTT CTTGCTGTTT CTCATCTGGC AAATTATGAA 
CAAGAATACA AGCTTCCTCT ATCACAAATC AACTTCTCAG GGGAGGAATG TGAGGATTCT 
GAACTTGTAA ACCACTTGAT GAACTGCACC AAAAGGAGAA GTGCCATAAC CCCCTTTGCC 
TGCCTGTCTG GAAATACAGA TCATGACTTA ACTTTTGCAG CACATTTGGA CAGTATTTTG 
TTGCAAACTA GTCATGTGAG TGAAAAACAC ATTCCTCGCC TGCATTTGGA GAACACAGAT 
GCTTGTGGTA GAAAACTGTT ACTAAATGCC TATGCACTGG ATTTCTTCAA GCACAGCTCC 
ATAGATGCAC TGGAAACTGA TAATGGGTTT CACAAAGGAG ATGCTTTTTA CAAGTTACGA 
GATTTTTCTT TATGCATTGC AACTATAAGA GTTTCTTTAA AGGAAATGTG TGAAGATGAG 
GATGACCCCG TTGTGTTAGC TTTCGAGCAG CTGAATACCC TGTACAGAGA GAAACTGGAG 
ACTGCTTATC CCAGGCGCAG AATCTTTTAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx60 ( XM_031895460.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751320.1 Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X3, mRNA.

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CloneID	OXa49816
Clone ID Related Accession (Same CDS sequence)	XM_031895460.1
Accession Version	XM_031895460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX60 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAC AAGGATCTTA TTTAGGACAG AAGTCACTGG AGTCTCAAAA AGAACCAGAT 
CCTGAATCTT CTGTACCATG TGACCCAGAA CCAAAAAACA ATGCTGAATC CCAGGTGAAA 
AGTTCTGAAT TCCCCCATGG CATGTACTTA TATGTAATGG AGAAACTCTC AAGAGCTGTC 
TTTGCCAGCC TGCTTAATGA TTATGTGGAA TCTGAATTCT TCTTGATTGA TGGAGATTCC 
TTACTGCTAA CTTGTGTTTT GGATCAGTCA CTGAAGGCAG GGCAGCATCT GCATTTCTTT 
TACTTGGTAG AATCCTACTT GCATAAACTG ACCAACCGGG GAGCAAAATA TGCTGTTGTG 
TTTTTTGAGG ATATGGAGTT TTTCTACCAT GACAGAGTCG ACCTTATCTC TCTACGGACC 
CAACTGAAAC TGCACTTGGA ACACAACACA GACACAGTCA TATACACCTT TTCCAACTTT 
TTGTCTGCTC AGTGGAACAC ATTTCTGATT GATCACCATC CCTACTTTCT AATGGTTTGT 
GATGAAGGTG TTACCCTGTC TCAAACATAT TTCTTCAGTA TGCTTATGCT AAGTGCCCTG 
GGGAAAAAAA TAGACCTCGT ACTCACGTCA GGACAAGAGT TCGATCAGCT CCGAGTGTAT 
GGATACCATA TCAATGCCAC CCACCATCAT AACTTATATC CCATAAAGAA ACAGAAGCAG 
TTTCTTCAAG ATTTGGTAGA TCCATGTGAA AAGTTGTACA GAAAGCGTGC ACAGAAGCTG 
AATTTCAGTA ACACCATAAA CGAGATTGAA AAGAGTATAA CATTACTTTT TAACAATGGG 
GATAAGGCTT TAGACATAAG AAGCATCATG TGTATTCTGT CCTGCTCTGC AGTGCTGACA 
ATATATGGTA AAAAAGCAAC ACATGGAGAA TGTCAGGCCT CACAGGATGA CAGTGACCTG 
ACCTTGGAAG AAGTGGCTGA CCTATGCAGA ATGTACTGTC TGAGTGTGGC CATATTGCTG 
GTTTTGCCCC TTTCCCAGAG AATGAAATCT AGGAACATCC ATGCAGATTG GAACAGCCCA 
GTATTAGCAT GTTTAGATTG GCAAATAATA TGTGAGCACA TTATTCTGAA GAAGTTTTCC 
AACACCTCAA ACTGGAAAAT AAATTGGACA CACATTGCTG ATCTAAGTGA TGGTTTGCTG 
TTGAAAAACA TTGCATATTA CTATGAAAAA GAGAACAATA CAGGCTCCAA GTTGATATTC 
GGGAAGGCAA TAGAGGAGAA GTTTTCATTT CTCTGGAACT CCATTTTGGA AATTAATCCA 
CATAGTGAAC AAATACAATC TTATGACGTC AGAACAACAT CACGTCCTTT CCTTTCAAAG 
GCTTCCTCAA TAATTGTGAG AGAGGAGAAC ATGCCAACTA TAGGTCTTCT ACGAGCAAAA 
TCTGACATTG TTGAAGAGTA TGCTGGGGAT TTTTTGGATG AATTACCATT TTTAAGCAGT 
ATTGAACATA AAGAGACCCC AGTCACTCGA GGGAAATATT TTGATGAACT TACACACTGG 
CATTCTGGAC GTCCACTAAG TGATGATTAT GACAGAACAA AGGGAGATGA GGCAGCATTG 
AAAGATGAAC GTGCTCTCAG AGATTACCAA AAGCTGCAAA CTCTTCACCG TTTTAGCGGG 
CAATCTTTAG TCTCCTCATC ATCCAAGACA ATTGTAATAC AGAATGATGA CCCTGAAAAG 
AAGGGGGAGA AAGAAAAGGT TATTGTTAAG AAAAAGCAAC CACAGAAGTC TAAAAAAGAT 
CTAATCATAG AGGAAAACCA GAAAAGAATG AATGCCAAAG AGGAAACTAA AGACGCTGAA 
CAGTGGAAAT TAGTGGCATC TTCAATTGAA AAAGAAATGA GAGCAAATTT TTTTCCTGGC 
TTAAAAAAAC TGGAAAGTTT CATAAAAAAT ACCCAAACCC GCTCAGTGAA GCTTCTTGCA 
GAACTATTAG GATTAAGAAT TTCCTTTAAG GTGTGGGTCG AGCACTGCTG CACAGACATG 
TCCTCAGAAG AGGAGGACGA TTTAAATATT GCTGTGGAAA TCATGAAGAA GATACAAACA 
CTTCATTATA AATACAAGGA CATGCTGACT AATGAGGATC AACAACTAAT AGCTCAATGT 
CTTAGTTATC TGGGATTTGA GAACTTGGCG TGCACATTGC CGCGCGCTAA GGTAACCAGT 
GCCAGAGAGG ACAAGAAGAA GGCAGAGTGC ATGGTTGGAG TTGGAGCAGC TCGTTTCCAA 
TTGCAGTTTA TGAGTCCATA CTTGGTAAGA GAGGAGAGGA CAGATCCTGA TCCAAGAGTA 
CAACACTTTA TACCGGACAC ATGGCAGCGT AAACTTCTTG ATGTTGTTGA CAATAATGAG 
TCTGCGGTGA TAGTTGCCCC TACATCTTCA GGAAAGACTT ACGCTTCGTA CTACTGCATG 
CAGAAAGTTC TGAGTCAAGG CCATGAAGGT GTGGTTGTGT ATGTTTCACC AACAAAGGCT 
CTTGTAAACC AGGTGGTAAC CACTGTAATG AATCAGTTCA ACAAAAATTT GCCCAATGGA 
GTGGCTCTGT GTGGTGTTTT CACAAGGGAT TACCGTACTG ATGCATTAAA TTCTCAGATA 
TTAGTAACAG TGCCTCAGTG TCTTGAGATT CTTCTCTTGT CGCCACACCG TCAGGAATGG 
GCTAAAAGGA TTAAGTATGT AATATTTGAT GAGATTCATT GCCTGGGTGG AGAAATAGGT 
GCAGAAGTAT GGGAACATCT TCTAGTGATG ATTCGCTGTC CATTTCTTGC TTTGTCTGCA 
ACAATAAGTA ACCCGGAACA TCTAACTGAA TGGCTGCAAT CAGTCAAATT TTACTGGCAA 
AAAAGAGATA AATTAATAGA GGAAAACTGG ACTGAGAAAG GAGCAAAGGC AAAAGCCTAT 
AAGAAGAAAG AAAAAATGTC TTACAGAGTT CATCTGGTGA TTTATAACAA GAGATACAAT 
GATCTGGAAA AATATGTGTG CTCTGTGCAA GACTCCAATG TATCGTTTGA GCACTATCAC 
CCATGTGCTG CCTTAACCAC GGACCACATG GAGAAATATG GAATTCCAAA TGACCTGTCC 
TGCTCCCCAA GGGAGAGTTT ACAACTGTAT GACACTATGG TGGATGTGTG GCCTGAATGG 
GCAAGGAGAG AGGAACTCGA TCCTGAAGAA TACGCACATT TCAAGGACAG AATCATCATA 
ACAAAAAATG ATACAAAAAT ATATGAGGAA GAACTTAAAA AAGAATTAAT TGACTGGATT 
AAGCAAGGAA ACCAGGAAAA GGTTTCCCAA CTATTGAAGT ATCTTCAGCC ACCAAAATCA 
TCTTGTAATG TGGACCGTGT CGCCTACTTT CCAAAGCTTG TTGAAAAACT GAAAGAAGAG 
CAAAAGCTGC CAGCTCTTCT TTTTGCATTT AACATTGGAC TTGTGGAACA TCTCGCACAG 
GAAACAGCCA TATTTCTAAA AAACAAGCAA GACAATAAGA CAAAACTTCT GACTGAGAAA 
AAAAGCCAAA AACTTATCAC CAAAGCAAAA AGACTGGAAA AAGCAATAGC AAAAAATGAA 
CCCTCAAAAG TAAACGACGC TTTCTTAGCC AATGTTGCCA ATCATAAGAG TATAGAGCAA 
GTACTTATAA AGGACAAAGA AATCCCACCA GACTGTACTT ATGCTGATAC CAGAGCTGTG 
AACAATGAGT TACTCTGGGA GGTGTTCAGA CAAACAAGTC GATCAAGAGA TGCCAAGTGT 
CTGAGGTTTC TGGCAGAAAG AGGTGTGGGC TACCATCATG CCTCTTTGGA TGTTAAAGGA 
AGAAGACAAG TGGAGATGCT CTTCAGGATG GGATACCTAC GGGTTGTTAC TGCAACTGGA 
TCTCTTGCCT TGGGAATCAA CATGCCGTGT AAATCTGTTG TGTTTGTTCA AGATTCACTT 
TACCTGGATG CATTAAATTA CAGACAGATG TCAGGACGTG CAGGAAGGCG AGGACAGGAT 
GTACTGGGAA ATGTATACTT TTTCACCATT CCTATGCCTA AAGTGAAACG GCTTATGAAA 
TCAGATGTCC CTAACTTAAA AGGACAGTTC CCTTTAGGCA TCTCTTTTGT CCTACGACTG 
ATGTTATTGG CTGCCAAAGC TGATCACAAA GAAGATGCCA GTGCTAAGGC TTTGTCAGTA 
CTGAAGCACT CCCTGATGGC GTTCAAAAAA CCTTCTGAGA CCAAAATGTT GAAGCTCTAC 
TTTCTGTTCT GCATACACTT CTTAATCCAT GAGGGCTATT TGGATCAACA TTGCAACCCT 
TTGGACTTTA CCGGGCTGGT TACACATTTA CATTACCATG AACCATCAAA TTTTGTGTTT 
GTCAGTTTTC TGGAAAATGG TTTATTTCAC CGGCTGTGCC AGAGAAACAC TAAAGGTTCA 
AATAGGTTTC CACAATCTGT GATGGAAACC CTGGTGCTGG TATTGGCTAA CTTGTTTGGA 
AGAAGCTACC TTTTACCATC CATGCTAGAA TTAAAGGGAA CATTTAAACA GTCAAAGGTC 
TTTCTGGAAG GTCTACCAGA TGATTTTGCT GCAGCTCTTG AAGACTACAA TTCCAAAATA 
TCAGCAGTCT TTGGGCAGTA TCTTCTTGCT GTTTCTCATC TGGCAAATTA TGAACAAGAA 
TACAAGCTTC CTCTATCACA AATCAACTTC TCAGGGGAGG AATGTGAGGA TTCTGAACTT 
GTAAACCACT TGATGAACTG CACCAAAAGG AGAAGTGCCA TAACCCCCTT TGCCTGCCTG 
TCTGGAAATA CAGATCATGA CTTAACTTTT GCAGCACATT TGGACAGTAT TTTGTTGCAA 
ACTAGTCATG TGAGTGAAAA ACACATTCCT CGCCTGCATT TGGAGAACAC AGATGCTTGT 
GGTAGAAAAC TGTTACTAAA TGCCTATGCA CTGGATTTCT TCAAGCACAG CTCCATAGAT 
GCACTGGAAA CTGATAATGG GTTTCACAAA GGAGATGCTT TTTACAAGTT ACGAGATTTT 
TCTTTATGCA TTGCAACTAT AAGAGTTTCT TTAAAGGAAA TGTGTGAAGA TGAGGATGAC 
CCCGTTGTGT TAGCTTTCGA GCAGCTGAAT ACCCTGTACA GAGAGAAACT GGAGACTGCT 
TATCCCAGGC GCAGAATCTT TTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751320.1
CDS	126..5315
Translation	MATQGSYLGQKSLESQKEPDPESSVPCDPEPKNNAESQVKSSEFPHGMYLYVMEKLSRAVFASLLNDYVESEFFLIDGDSLLLTCVLDQSLKAGQHLHFFYLVESYLHKLTNRGAKYAVVFFEDMEFFYHDRVDLISLRTQLKLHLEHNTDTVIYTFSNFLSAQWNTFLIDHHPYFLMVCDEGVTLSQTYFFSMLMLSALGKKIDLVLTSGQEFDQLRVYGYHINATHHHNLYPIKKQKQFLQDLVDPCEKLYRKRAQKLNFSNTINEIEKSITLLFNNGDKALDIRSIMCILSCSAVLTIYGKKATHGECQASQDDSDLTLEEVADLCRMYCLSVAILLVLPLSQRMKSRNIHADWNSPVLACLDWQIICEHIILKKFSNTSNWKINWTHIADLSDGLLLKNIAYYYEKENNTGSKLIFGKAIEEKFSFLWNSILEINPHSEQIQSYDVRTTSRPFLSKASSIIVREENMPTIGLLRAKSDIVEEYAGDFLDELPFLSSIEHKETPVTRGKYFDELTHWHSGRPLSDDYDRTKGDEAALKDERALRDYQKLQTLHRFSGQSLVSSSSKTIVIQNDDPEKKGEKEKVIVKKKQPQKSKKDLIIEENQKRMNAKEETKDAEQWKLVASSIEKEMRANFFPGLKKLESFIKNTQTRSVKLLAELLGLRISFKVWVEHCCTDMSSEEEDDLNIAVEIMKKIQTLHYKYKDMLTNEDQQLIAQCLSYLGFENLACTLPRAKVTSAREDKKKAECMVGVGAARFQLQFMSPYLVREERTDPDPRVQHFIPDTWQRKLLDVVDNNESAVIVAPTSSGKTYASYYCMQKVLSQGHEGVVVYVSPTKALVNQVVTTVMNQFNKNLPNGVALCGVFTRDYRTDALNSQILVTVPQCLEILLLSPHRQEWAKRIKYVIFDEIHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKFYWQKRDKLIEENWTEKGAKAKAYKKKEKMSYRVHLVIYNKRYNDLEKYVCSVQDSNVSFEHYHPCAALTTDHMEKYGIPNDLSCSPRESLQLYDTMVDVWPEWARREELDPEEYAHFKDRIIITKNDTKIYEEELKKELIDWIKQGNQEKVSQLLKYLQPPKSSCNVDRVAYFPKLVEKLKEEQKLPALLFAFNIGLVEHLAQETAIFLKNKQDNKTKLLTEKKSQKLITKAKRLEKAIAKNEPSKVNDAFLANVANHKSIEQVLIKDKEIPPDCTYADTRAVNNELLWEVFRQTSRSRDAKCLRFLAERGVGYHHASLDVKGRRQVEMLFRMGYLRVVTATGSLALGINMPCKSVVFVQDSLYLDALNYRQMSGRAGRRGQDVLGNVYFFTIPMPKVKRLMKSDVPNLKGQFPLGISFVLRLMLLAAKADHKEDASAKALSVLKHSLMAFKKPSETKMLKLYFLFCIHFLIHEGYLDQHCNPLDFTGLVTHLHYHEPSNFVFVSFLENGLFHRLCQRNTKGSNRFPQSVMETLVLVLANLFGRSYLLPSMLELKGTFKQSKVFLEGLPDDFAAALEDYNSKISAVFGQYLLAVSHLANYEQEYKLPLSQINFSGEECEDSELVNHLMNCTKRRSAITPFACLSGNTDHDLTFAAHLDSILLQTSHVSEKHIPRLHLENTDACGRKLLLNAYALDFFKHSSIDALETDNGFHKGDAFYKLRDFSLCIATIRVSLKEMCEDEDDPVVLAFEQLNTLYREKLETAYPRRRIFYA

Target ORF information:

RefSeq Version	XM_031895460.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895460.1

ORF Insert Sequence:

ATGGCTACAC AAGGATCTTA TTTAGGACAG AAGTCACTGG AGTCTCAAAA AGAACCAGAT 
CCTGAATCTT CTGTACCATG TGACCCAGAA CCAAAAAACA ATGCTGAATC CCAGGTGAAA 
AGTTCTGAAT TCCCCCATGG CATGTACTTA TATGTAATGG AGAAACTCTC AAGAGCTGTC 
TTTGCCAGCC TGCTTAATGA TTATGTGGAA TCTGAATTCT TCTTGATTGA TGGAGATTCC 
TTACTGCTAA CTTGTGTTTT GGATCAGTCA CTGAAGGCAG GGCAGCATCT GCATTTCTTT 
TACTTGGTAG AATCCTACTT GCATAAACTG ACCAACCGGG GAGCAAAATA TGCTGTTGTG 
TTTTTTGAGG ATATGGAGTT TTTCTACCAT GACAGAGTCG ACCTTATCTC TCTACGGACC 
CAACTGAAAC TGCACTTGGA ACACAACACA GACACAGTCA TATACACCTT TTCCAACTTT 
TTGTCTGCTC AGTGGAACAC ATTTCTGATT GATCACCATC CCTACTTTCT AATGGTTTGT 
GATGAAGGTG TTACCCTGTC TCAAACATAT TTCTTCAGTA TGCTTATGCT AAGTGCCCTG 
GGGAAAAAAA TAGACCTCGT ACTCACGTCA GGACAAGAGT TCGATCAGCT CCGAGTGTAT 
GGATACCATA TCAATGCCAC CCACCATCAT AACTTATATC CCATAAAGAA ACAGAAGCAG 
TTTCTTCAAG ATTTGGTAGA TCCATGTGAA AAGTTGTACA GAAAGCGTGC ACAGAAGCTG 
AATTTCAGTA ACACCATAAA CGAGATTGAA AAGAGTATAA CATTACTTTT TAACAATGGG 
GATAAGGCTT TAGACATAAG AAGCATCATG TGTATTCTGT CCTGCTCTGC AGTGCTGACA 
ATATATGGTA AAAAAGCAAC ACATGGAGAA TGTCAGGCCT CACAGGATGA CAGTGACCTG 
ACCTTGGAAG AAGTGGCTGA CCTATGCAGA ATGTACTGTC TGAGTGTGGC CATATTGCTG 
GTTTTGCCCC TTTCCCAGAG AATGAAATCT AGGAACATCC ATGCAGATTG GAACAGCCCA 
GTATTAGCAT GTTTAGATTG GCAAATAATA TGTGAGCACA TTATTCTGAA GAAGTTTTCC 
AACACCTCAA ACTGGAAAAT AAATTGGACA CACATTGCTG ATCTAAGTGA TGGTTTGCTG 
TTGAAAAACA TTGCATATTA CTATGAAAAA GAGAACAATA CAGGCTCCAA GTTGATATTC 
GGGAAGGCAA TAGAGGAGAA GTTTTCATTT CTCTGGAACT CCATTTTGGA AATTAATCCA 
CATAGTGAAC AAATACAATC TTATGACGTC AGAACAACAT CACGTCCTTT CCTTTCAAAG 
GCTTCCTCAA TAATTGTGAG AGAGGAGAAC ATGCCAACTA TAGGTCTTCT ACGAGCAAAA 
TCTGACATTG TTGAAGAGTA TGCTGGGGAT TTTTTGGATG AATTACCATT TTTAAGCAGT 
ATTGAACATA AAGAGACCCC AGTCACTCGA GGGAAATATT TTGATGAACT TACACACTGG 
CATTCTGGAC GTCCACTAAG TGATGATTAT GACAGAACAA AGGGAGATGA GGCAGCATTG 
AAAGATGAAC GTGCTCTCAG AGATTACCAA AAGCTGCAAA CTCTTCACCG TTTTAGCGGG 
CAATCTTTAG TCTCCTCATC ATCCAAGACA ATTGTAATAC AGAATGATGA CCCTGAAAAG 
AAGGGGGAGA AAGAAAAGGT TATTGTTAAG AAAAAGCAAC CACAGAAGTC TAAAAAAGAT 
CTAATCATAG AGGAAAACCA GAAAAGAATG AATGCCAAAG AGGAAACTAA AGACGCTGAA 
CAGTGGAAAT TAGTGGCATC TTCAATTGAA AAAGAAATGA GAGCAAATTT TTTTCCTGGC 
TTAAAAAAAC TGGAAAGTTT CATAAAAAAT ACCCAAACCC GCTCAGTGAA GCTTCTTGCA 
GAACTATTAG GATTAAGAAT TTCCTTTAAG GTGTGGGTCG AGCACTGCTG CACAGACATG 
TCCTCAGAAG AGGAGGACGA TTTAAATATT GCTGTGGAAA TCATGAAGAA GATACAAACA 
CTTCATTATA AATACAAGGA CATGCTGACT AATGAGGATC AACAACTAAT AGCTCAATGT 
CTTAGTTATC TGGGATTTGA GAACTTGGCG TGCACATTGC CGCGCGCTAA GGTAACCAGT 
GCCAGAGAGG ACAAGAAGAA GGCAGAGTGC ATGGTTGGAG TTGGAGCAGC TCGTTTCCAA 
TTGCAGTTTA TGAGTCCATA CTTGGTAAGA GAGGAGAGGA CAGATCCTGA TCCAAGAGTA 
CAACACTTTA TACCGGACAC ATGGCAGCGT AAACTTCTTG ATGTTGTTGA CAATAATGAG 
TCTGCGGTGA TAGTTGCCCC TACATCTTCA GGAAAGACTT ACGCTTCGTA CTACTGCATG 
CAGAAAGTTC TGAGTCAAGG CCATGAAGGT GTGGTTGTGT ATGTTTCACC AACAAAGGCT 
CTTGTAAACC AGGTGGTAAC CACTGTAATG AATCAGTTCA ACAAAAATTT GCCCAATGGA 
GTGGCTCTGT GTGGTGTTTT CACAAGGGAT TACCGTACTG ATGCATTAAA TTCTCAGATA 
TTAGTAACAG TGCCTCAGTG TCTTGAGATT CTTCTCTTGT CGCCACACCG TCAGGAATGG 
GCTAAAAGGA TTAAGTATGT AATATTTGAT GAGATTCATT GCCTGGGTGG AGAAATAGGT 
GCAGAAGTAT GGGAACATCT TCTAGTGATG ATTCGCTGTC CATTTCTTGC TTTGTCTGCA 
ACAATAAGTA ACCCGGAACA TCTAACTGAA TGGCTGCAAT CAGTCAAATT TTACTGGCAA 
AAAAGAGATA AATTAATAGA GGAAAACTGG ACTGAGAAAG GAGCAAAGGC AAAAGCCTAT 
AAGAAGAAAG AAAAAATGTC TTACAGAGTT CATCTGGTGA TTTATAACAA GAGATACAAT 
GATCTGGAAA AATATGTGTG CTCTGTGCAA GACTCCAATG TATCGTTTGA GCACTATCAC 
CCATGTGCTG CCTTAACCAC GGACCACATG GAGAAATATG GAATTCCAAA TGACCTGTCC 
TGCTCCCCAA GGGAGAGTTT ACAACTGTAT GACACTATGG TGGATGTGTG GCCTGAATGG 
GCAAGGAGAG AGGAACTCGA TCCTGAAGAA TACGCACATT TCAAGGACAG AATCATCATA 
ACAAAAAATG ATACAAAAAT ATATGAGGAA GAACTTAAAA AAGAATTAAT TGACTGGATT 
AAGCAAGGAA ACCAGGAAAA GGTTTCCCAA CTATTGAAGT ATCTTCAGCC ACCAAAATCA 
TCTTGTAATG TGGACCGTGT CGCCTACTTT CCAAAGCTTG TTGAAAAACT GAAAGAAGAG 
CAAAAGCTGC CAGCTCTTCT TTTTGCATTT AACATTGGAC TTGTGGAACA TCTCGCACAG 
GAAACAGCCA TATTTCTAAA AAACAAGCAA GACAATAAGA CAAAACTTCT GACTGAGAAA 
AAAAGCCAAA AACTTATCAC CAAAGCAAAA AGACTGGAAA AAGCAATAGC AAAAAATGAA 
CCCTCAAAAG TAAACGACGC TTTCTTAGCC AATGTTGCCA ATCATAAGAG TATAGAGCAA 
GTACTTATAA AGGACAAAGA AATCCCACCA GACTGTACTT ATGCTGATAC CAGAGCTGTG 
AACAATGAGT TACTCTGGGA GGTGTTCAGA CAAACAAGTC GATCAAGAGA TGCCAAGTGT 
CTGAGGTTTC TGGCAGAAAG AGGTGTGGGC TACCATCATG CCTCTTTGGA TGTTAAAGGA 
AGAAGACAAG TGGAGATGCT CTTCAGGATG GGATACCTAC GGGTTGTTAC TGCAACTGGA 
TCTCTTGCCT TGGGAATCAA CATGCCGTGT AAATCTGTTG TGTTTGTTCA AGATTCACTT 
TACCTGGATG CATTAAATTA CAGACAGATG TCAGGACGTG CAGGAAGGCG AGGACAGGAT 
GTACTGGGAA ATGTATACTT TTTCACCATT CCTATGCCTA AAGTGAAACG GCTTATGAAA 
TCAGATGTCC CTAACTTAAA AGGACAGTTC CCTTTAGGCA TCTCTTTTGT CCTACGACTG 
ATGTTATTGG CTGCCAAAGC TGATCACAAA GAAGATGCCA GTGCTAAGGC TTTGTCAGTA 
CTGAAGCACT CCCTGATGGC GTTCAAAAAA CCTTCTGAGA CCAAAATGTT GAAGCTCTAC 
TTTCTGTTCT GCATACACTT CTTAATCCAT GAGGGCTATT TGGATCAACA TTGCAACCCT 
TTGGACTTTA CCGGGCTGGT TACACATTTA CATTACCATG AACCATCAAA TTTTGTGTTT 
GTCAGTTTTC TGGAAAATGG TTTATTTCAC CGGCTGTGCC AGAGAAACAC TAAAGGTTCA 
AATAGGTTTC CACAATCTGT GATGGAAACC CTGGTGCTGG TATTGGCTAA CTTGTTTGGA 
AGAAGCTACC TTTTACCATC CATGCTAGAA TTAAAGGGAA CATTTAAACA GTCAAAGGTC 
TTTCTGGAAG GTCTACCAGA TGATTTTGCT GCAGCTCTTG AAGACTACAA TTCCAAAATA 
TCAGCAGTCT TTGGGCAGTA TCTTCTTGCT GTTTCTCATC TGGCAAATTA TGAACAAGAA 
TACAAGCTTC CTCTATCACA AATCAACTTC TCAGGGGAGG AATGTGAGGA TTCTGAACTT 
GTAAACCACT TGATGAACTG CACCAAAAGG AGAAGTGCCA TAACCCCCTT TGCCTGCCTG 
TCTGGAAATA CAGATCATGA CTTAACTTTT GCAGCACATT TGGACAGTAT TTTGTTGCAA 
ACTAGTCATG TGAGTGAAAA ACACATTCCT CGCCTGCATT TGGAGAACAC AGATGCTTGT 
GGTAGAAAAC TGTTACTAAA TGCCTATGCA CTGGATTTCT TCAAGCACAG CTCCATAGAT 
GCACTGGAAA CTGATAATGG GTTTCACAAA GGAGATGCTT TTTACAAGTT ACGAGATTTT 
TCTTTATGCA TTGCAACTAT AAGAGTTTCT TTAAAGGAAA TGTGTGAAGA TGAGGATGAC 
CCCGTTGTGT TAGCTTTCGA GCAGCTGAAT ACCCTGTACA GAGAGAAACT GGAGACTGCT 
TATCCCAGGC GCAGAATCTT TTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx60 ( XM_031895461.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751321.1 Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X4, mRNA.

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CloneID	OXa49817
Clone ID Related Accession (Same CDS sequence)	XM_031895461.1 , XM_031895462.1
Accession Version	XM_031895461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX60 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AAGGGTCTCA TTTGGAAAAG ATCTCACTGG ACTCTCAAAC AGAGATTTCT 
GTATCACATG AAGCAGAACC AAAGACTAAA AATGAATCCC GAATGAAAAA TGATGACGCC 
CTTCGTGACT TGTTCACTTT TACAGTGCAG AAACTCTCAG GAGCTGTCTT TGCCAGCCTG 
CTTAATGATT ATGTGGAATC TGAATTCTTC TTGATTGATG GAGATTCCTT ACTGCTAACT 
TGTGTTTTGG ATCAGTCACT GAAGGCAGGG CAGCATCTGC ATTTCTTTTA CTTGGTAGAA 
TCCTACTTGC ATAAACTGAC CAACCGGGGA GCAAAATATG CTGTTGTGTT TTTTGAGGAT 
ATGGAGTTTT TCTACCATGA CAGAGTCGAC CTTATCTCTC TACGGACCCA ACTGAAACTG 
CACTTGGAAC ACAACACAGA CACAGTCATA TACACCTTTT CCAACTTTTT GTCTGCTCAG 
TGGAACACAT TTCTGATTGA TCACCATCCC TACTTTCTAA TGGTTTGTGA TGAAGGTGTT 
ACCCTGTCTC AAACATATTT CTTCAGTATG CTTATGCTAA GTGCCCTGGG GAAAAAAATA 
GACCTCGTAC TCACGTCAGG ACAAGAGTTC GATCAGCTCC GAGTGTATGG ATACCATATC 
AATGCCACCC ACCATCATAA CTTATATCCC ATAAAGAAAC AGAAGCAGTT TCTTCAAGAT 
TTGGTAGATC CATGTGAAAA GTTGTACAGA AAGCGTGCAC AGAAGCTGAA TTTCAGTAAC 
ACCATAAACG AGATTGAAAA GAGTATAACA TTACTTTTTA ACAATGGGGA TAAGGCTTTA 
GACATAAGAA GCATCATGTG TATTCTGTCC TGCTCTGCAG TGCTGACAAT ATATGGTAAA 
AAAGCAACAC ATGGAGAATG TCAGGCCTCA CAGGTACAGG ATGACAGTGA CCTGACCTTG 
GAAGAAGTGG CTGACCTATG CAGAATGTAC TGTCTGAGTG TGGCCATATT GCTGGTTTTG 
CCCCTTTCCC AGAGAATGAA ATCTAGGAAC ATCCATGCAG ATTGGAACAG CCCAGTATTA 
GCATGTTTAG ATTGGCAAAT AATATGTGAG CACATTATTC TGAAGAAGTT TTCCAACACC 
TCAAACTGGA AAATAAATTG GACACACATT GCTGATCTAA GTGATGGTTT GCTGTTGAAA 
AACATTGCAT ATTACTATGA AAAAGAGAAC AATACAGGCT CCAAGTTGAT ATTCGGGAAG 
GCAATAGAGG AGAAGTTTTC ATTTCTCTGG AACTCCATTT TGGAAATTAA TCCACATAGT 
GAACAAATAC AATCTTATGA CGTCAGAACA ACATCACGTC CTTTCCTTTC AAAGGCTTCC 
TCAATAATTG TGAGAGAGGA GAACATGCCA ACTATAGGTC TTCTACGAGC AAAATCTGAC 
ATTGTTGAAG AGTATGCTGG GGATTTTTTG GATGAATTAC CATTTTTAAG CAGTATTGAA 
CATAAAGAGA CCCCAGTCAC TCGAGGGAAA TATTTTGATG AACTTACACA CTGGCATTCT 
GGACGTCCAC TAAGTGATGA TTATGACAGA ACAAAGGGAG ATGAGGCAGC ATTGAAAGAT 
GAACGTGCTC TCAGAGATTA CCAAAAGCTG CAAACTCTTC ACCGTTTTAG CGGGCAATCT 
TTAGTCTCCT CATCATCCAA GACAATTGTA ATACAGAATG ATGACCCTGA AAAGAAGGGG 
GAGAAAGAAA AGGTTATTGT TAAGAAAAAG CAACCACAGA AGTCTAAAAA AGATCTAATC 
ATAGAGGAAA ACCAGAAAAG AATGAATGCC AAAGAGGAAA CTAAAGACGC TGAACAGTGG 
AAATTAGTGG CATCTTCAAT TGAAAAAGAA ATGAGAGCAA ATTTTTTTCC TGGCTTAAAA 
AAACTGGAAA GTTTCATAAA AAATACCCAA ACCCGCTCAG TGAAGCTTCT TGCAGAACTA 
TTAGGATTAA GAATTTCCTT TAAGGTGTGG GTCGAGCACT GCTGCACAGA CATGTCCTCA 
GAAGAGGAGG ACGATTTAAA TATTGCTGTG GAAATCATGA AGAAGATACA AACACTTCAT 
TATAAATACA AGGACATGCT GACTAATGAG GATCAACAAC TAATAGCTCA ATGTCTTAGT 
TATCTGGGAT TTGAGAACTT GGCGTGCACA TTGCCGCGCG CTAAGGTAAC CAGTGCCAGA 
GAGGACAAGA AGAAGGCAGA GTGCATGGTT GGAGTTGGAG CAGCTCGTTT CCAATTGCAG 
TTTATGAGTC CATACTTGGT AAGAGAGGAG AGGACAGATC CTGATCCAAG AGTACAACAC 
TTTATACCGG ACACATGGCA GCGTAAACTT CTTGATGTTG TTGACAATAA TGAGTCTGCG 
GTGATAGTTG CCCCTACATC TTCAGGAAAG ACTTACGCTT CGTACTACTG CATGCAGAAA 
GTTCTGAGTC AAGGCCATGA AGGTGTGGTT GTGTATGTTT CACCAACAAA GGCTCTTGTA 
AACCAGGTGG TAACCACTGT AATGAATCAG TTCAACAAAA ATTTGCCCAA TGGAGTGGCT 
CTGTGTGGTG TTTTCACAAG GGATTACCGT ACTGATGCAT TAAATTCTCA GATATTAGTA 
ACAGTGCCTC AGTGTCTTGA GATTCTTCTC TTGTCGCCAC ACCGTCAGGA ATGGGCTAAA 
AGGATTAAGT ATGTAATATT TGATGAGATT CATTGCCTGG GTGGAGAAAT AGGTGCAGAA 
GTATGGGAAC ATCTTCTAGT GATGATTCGC TGTCCATTTC TTGCTTTGTC TGCAACAATA 
AGTAACCCGG AACATCTAAC TGAATGGCTG CAATCAGTCA AATTTTACTG GCAAAAAAGA 
GATAAATTAA TAGAGGAAAA CTGGACTGAG AAAGGAGCAA AGGCAAAAGC CTATAAGAAG 
AAAGAAAAAA TGTCTTACAG AGTTCATCTG GTGATTTATA ACAAGAGATA CAATGATCTG 
GAAAAATATG TGTGCTCTGT GCAAGACTCC AATGTATCGT TTGAGCACTA TCACCCATGT 
GCTGCCTTAA CCACGGACCA CATGGAGAAA TATGGAATTC CAAATGACCT GTCCTGCTCC 
CCAAGGGAGA GTTTACAACT GTATGACACT ATGGTGGATG TGTGGCCTGA ATGGGCAAGG 
AGAGAGGAAC TCGATCCTGA AGAATACGCA CATTTCAAGG ACAGAATCAT CATAACAAAA 
AATGATACAA AAATATATGA GGAAGAACTT AAAAAAGAAT TAATTGACTG GATTAAGCAA 
GGAAACCAGG AAAAGGTTTC CCAACTATTG AAGTATCTTC AGCCACCAAA ATCATCTTGT 
AATGTGGACC GTGTCGCCTA CTTTCCAAAG CTTGTTGAAA AACTGAAAGA AGAGCAAAAG 
CTGCCAGCTC TTCTTTTTGC ATTTAACATT GGACTTGTGG AACATCTCGC ACAGGAAACA 
GCCATATTTC TAAAAAACAA GCAAGACAAT AAGACAAAAC TTCTGACTGA GAAAAAAAGC 
CAAAAACTTA TCACCAAAGC AAAAAGACTG GAAAAAGCAA TAGCAAAAAA TGAACCCTCA 
AAAGTAAACG ACGCTTTCTT AGCCAATGTT GCCAATCATA AGAGTATAGA GCAAGTACTT 
ATAAAGGACA AAGAAATCCC ACCAGACTGT ACTTATGCTG ATACCAGAGC TGTGAACAAT 
GAGTTACTCT GGGAGGTGTT CAGACAAACA AGTCGATCAA GAGATGCCAA GTGTCTGAGG 
TTTCTGGCAG AAAGAGGTGT GGGCTACCAT CATGCCTCTT TGGATGTTAA AGGAAGAAGA 
CAAGTGGAGA TGCTCTTCAG GATGGGATAC CTACGGGTTG TTACTGCAAC TGGATCTCTT 
GCCTTGGGAA TCAACATGCC GTGTAAATCT GTTGTGTTTG TTCAAGATTC ACTTTACCTG 
GATGCATTAA ATTACAGACA GATGTCAGGA CGTGCAGGAA GGCGAGGACA GGATGTACTG 
GGAAATGTAT ACTTTTTCAC CATTCCTATG CCTAAAGTGA AACGGCTTAT GAAATCAGAT 
GTCCCTAACT TAAAAGGACA GTTCCCTTTA GGCATCTCTT TTGTCCTACG ACTGATGTTA 
TTGGCTGCCA AAGCTGATCA CAAAGAAGAT GCCAGTGCTA AGGCTTTGTC AGTACTGAAG 
CACTCCCTGA TGGCGTTCAA AAAACCTTCT GAGACCAAAA TGTTGAAGCT CTACTTTCTG 
TTCTGCATAC ACTTCTTAAT CCATGAGGGC TATTTGGATC AACATTGCAA CCCTTTGGAC 
TTTACCGGGC TGGTTACACA TTTACATTAC CATGAACCAT CAAATTTTGT GTTTGTCAGT 
TTTCTGGAAA ATGGTTTATT TCACCGGCTG TGCCAGAGAA ACACTAAAGG TTCAAATAGG 
TTTCCACAAT CTGTGATGGA AACCCTGGTG CTGGTATTGG CTAACTTGTT TGGAAGAAGC 
TACCTTTTAC CATCCATGCT AGAATTAAAG GGAACATTTA AACAGTCAAA GGTCTTTCTG 
GAAGGTCTAC CAGATGATTT TGCTGCAGCT CTTGAAGACT ACAATTCCAA AATATCAGCA 
GTCTTTGGGC AGTATCTTCT TGCTGTTTCT CATCTGGCAA ATTATGAACA AGAATACAAG 
CTTCCTCTAT CACAAATCAA CTTCTCAGGG GAGGAATGTG AGGATTCTGA ACTTGTAAAC 
CACTTGATGA ACTGCACCAA AAGGAGAAGT GCCATAACCC CCTTTGCCTG CCTGTCTGGA 
AATACAGATC ATGACTTAAC TTTTGCAGCA CATTTGGACA GTATTTTGTT GCAAACTAGT 
CATGTGAGTG AAAAACACAT TCCTCGCCTG CATTTGGAGA ACACAGATGC TTGTGGTAGA 
AAACTGTTAC TAAATGCCTA TGCACTGGAT TTCTTCAAGC ACAGCTCCAT AGATGCACTG 
GAAACTGATA ATGGGTTTCA CAAAGGAGAT GCTTTTTACA AGTTACGAGA TTTTTCTTTA 
TGCATTGCAA CTATAAGAGT TTCTTTAAAG GAAATGTGTG AAGATGAGGA TGACCCCGTT 
GTGTTAGCTT TCGAGCAGCT GAATACCCTG TACAGAGAGA AACTGGAGAC TGCTTATCCC 
AGGCGCAGAA TCTTTTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751321.1
CDS	558..5741
Translation	MATQGSHLEKISLDSQTEISVSHEAEPKTKNESRMKNDDALRDLFTFTVQKLSGAVFASLLNDYVESEFFLIDGDSLLLTCVLDQSLKAGQHLHFFYLVESYLHKLTNRGAKYAVVFFEDMEFFYHDRVDLISLRTQLKLHLEHNTDTVIYTFSNFLSAQWNTFLIDHHPYFLMVCDEGVTLSQTYFFSMLMLSALGKKIDLVLTSGQEFDQLRVYGYHINATHHHNLYPIKKQKQFLQDLVDPCEKLYRKRAQKLNFSNTINEIEKSITLLFNNGDKALDIRSIMCILSCSAVLTIYGKKATHGECQASQVQDDSDLTLEEVADLCRMYCLSVAILLVLPLSQRMKSRNIHADWNSPVLACLDWQIICEHIILKKFSNTSNWKINWTHIADLSDGLLLKNIAYYYEKENNTGSKLIFGKAIEEKFSFLWNSILEINPHSEQIQSYDVRTTSRPFLSKASSIIVREENMPTIGLLRAKSDIVEEYAGDFLDELPFLSSIEHKETPVTRGKYFDELTHWHSGRPLSDDYDRTKGDEAALKDERALRDYQKLQTLHRFSGQSLVSSSSKTIVIQNDDPEKKGEKEKVIVKKKQPQKSKKDLIIEENQKRMNAKEETKDAEQWKLVASSIEKEMRANFFPGLKKLESFIKNTQTRSVKLLAELLGLRISFKVWVEHCCTDMSSEEEDDLNIAVEIMKKIQTLHYKYKDMLTNEDQQLIAQCLSYLGFENLACTLPRAKVTSAREDKKKAECMVGVGAARFQLQFMSPYLVREERTDPDPRVQHFIPDTWQRKLLDVVDNNESAVIVAPTSSGKTYASYYCMQKVLSQGHEGVVVYVSPTKALVNQVVTTVMNQFNKNLPNGVALCGVFTRDYRTDALNSQILVTVPQCLEILLLSPHRQEWAKRIKYVIFDEIHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKFYWQKRDKLIEENWTEKGAKAKAYKKKEKMSYRVHLVIYNKRYNDLEKYVCSVQDSNVSFEHYHPCAALTTDHMEKYGIPNDLSCSPRESLQLYDTMVDVWPEWARREELDPEEYAHFKDRIIITKNDTKIYEEELKKELIDWIKQGNQEKVSQLLKYLQPPKSSCNVDRVAYFPKLVEKLKEEQKLPALLFAFNIGLVEHLAQETAIFLKNKQDNKTKLLTEKKSQKLITKAKRLEKAIAKNEPSKVNDAFLANVANHKSIEQVLIKDKEIPPDCTYADTRAVNNELLWEVFRQTSRSRDAKCLRFLAERGVGYHHASLDVKGRRQVEMLFRMGYLRVVTATGSLALGINMPCKSVVFVQDSLYLDALNYRQMSGRAGRRGQDVLGNVYFFTIPMPKVKRLMKSDVPNLKGQFPLGISFVLRLMLLAAKADHKEDASAKALSVLKHSLMAFKKPSETKMLKLYFLFCIHFLIHEGYLDQHCNPLDFTGLVTHLHYHEPSNFVFVSFLENGLFHRLCQRNTKGSNRFPQSVMETLVLVLANLFGRSYLLPSMLELKGTFKQSKVFLEGLPDDFAAALEDYNSKISAVFGQYLLAVSHLANYEQEYKLPLSQINFSGEECEDSELVNHLMNCTKRRSAITPFACLSGNTDHDLTFAAHLDSILLQTSHVSEKHIPRLHLENTDACGRKLLLNAYALDFFKHSSIDALETDNGFHKGDAFYKLRDFSLCIATIRVSLKEMCEDEDDPVVLAFEQLNTLYREKLETAYPRRRIFYA

Target ORF information:

RefSeq Version	XM_031895461.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895461.1

ORF Insert Sequence:

ATGGCCACAC AAGGGTCTCA TTTGGAAAAG ATCTCACTGG ACTCTCAAAC AGAGATTTCT 
GTATCACATG AAGCAGAACC AAAGACTAAA AATGAATCCC GAATGAAAAA TGATGACGCC 
CTTCGTGACT TGTTCACTTT TACAGTGCAG AAACTCTCAG GAGCTGTCTT TGCCAGCCTG 
CTTAATGATT ATGTGGAATC TGAATTCTTC TTGATTGATG GAGATTCCTT ACTGCTAACT 
TGTGTTTTGG ATCAGTCACT GAAGGCAGGG CAGCATCTGC ATTTCTTTTA CTTGGTAGAA 
TCCTACTTGC ATAAACTGAC CAACCGGGGA GCAAAATATG CTGTTGTGTT TTTTGAGGAT 
ATGGAGTTTT TCTACCATGA CAGAGTCGAC CTTATCTCTC TACGGACCCA ACTGAAACTG 
CACTTGGAAC ACAACACAGA CACAGTCATA TACACCTTTT CCAACTTTTT GTCTGCTCAG 
TGGAACACAT TTCTGATTGA TCACCATCCC TACTTTCTAA TGGTTTGTGA TGAAGGTGTT 
ACCCTGTCTC AAACATATTT CTTCAGTATG CTTATGCTAA GTGCCCTGGG GAAAAAAATA 
GACCTCGTAC TCACGTCAGG ACAAGAGTTC GATCAGCTCC GAGTGTATGG ATACCATATC 
AATGCCACCC ACCATCATAA CTTATATCCC ATAAAGAAAC AGAAGCAGTT TCTTCAAGAT 
TTGGTAGATC CATGTGAAAA GTTGTACAGA AAGCGTGCAC AGAAGCTGAA TTTCAGTAAC 
ACCATAAACG AGATTGAAAA GAGTATAACA TTACTTTTTA ACAATGGGGA TAAGGCTTTA 
GACATAAGAA GCATCATGTG TATTCTGTCC TGCTCTGCAG TGCTGACAAT ATATGGTAAA 
AAAGCAACAC ATGGAGAATG TCAGGCCTCA CAGGTACAGG ATGACAGTGA CCTGACCTTG 
GAAGAAGTGG CTGACCTATG CAGAATGTAC TGTCTGAGTG TGGCCATATT GCTGGTTTTG 
CCCCTTTCCC AGAGAATGAA ATCTAGGAAC ATCCATGCAG ATTGGAACAG CCCAGTATTA 
GCATGTTTAG ATTGGCAAAT AATATGTGAG CACATTATTC TGAAGAAGTT TTCCAACACC 
TCAAACTGGA AAATAAATTG GACACACATT GCTGATCTAA GTGATGGTTT GCTGTTGAAA 
AACATTGCAT ATTACTATGA AAAAGAGAAC AATACAGGCT CCAAGTTGAT ATTCGGGAAG 
GCAATAGAGG AGAAGTTTTC ATTTCTCTGG AACTCCATTT TGGAAATTAA TCCACATAGT 
GAACAAATAC AATCTTATGA CGTCAGAACA ACATCACGTC CTTTCCTTTC AAAGGCTTCC 
TCAATAATTG TGAGAGAGGA GAACATGCCA ACTATAGGTC TTCTACGAGC AAAATCTGAC 
ATTGTTGAAG AGTATGCTGG GGATTTTTTG GATGAATTAC CATTTTTAAG CAGTATTGAA 
CATAAAGAGA CCCCAGTCAC TCGAGGGAAA TATTTTGATG AACTTACACA CTGGCATTCT 
GGACGTCCAC TAAGTGATGA TTATGACAGA ACAAAGGGAG ATGAGGCAGC ATTGAAAGAT 
GAACGTGCTC TCAGAGATTA CCAAAAGCTG CAAACTCTTC ACCGTTTTAG CGGGCAATCT 
TTAGTCTCCT CATCATCCAA GACAATTGTA ATACAGAATG ATGACCCTGA AAAGAAGGGG 
GAGAAAGAAA AGGTTATTGT TAAGAAAAAG CAACCACAGA AGTCTAAAAA AGATCTAATC 
ATAGAGGAAA ACCAGAAAAG AATGAATGCC AAAGAGGAAA CTAAAGACGC TGAACAGTGG 
AAATTAGTGG CATCTTCAAT TGAAAAAGAA ATGAGAGCAA ATTTTTTTCC TGGCTTAAAA 
AAACTGGAAA GTTTCATAAA AAATACCCAA ACCCGCTCAG TGAAGCTTCT TGCAGAACTA 
TTAGGATTAA GAATTTCCTT TAAGGTGTGG GTCGAGCACT GCTGCACAGA CATGTCCTCA 
GAAGAGGAGG ACGATTTAAA TATTGCTGTG GAAATCATGA AGAAGATACA AACACTTCAT 
TATAAATACA AGGACATGCT GACTAATGAG GATCAACAAC TAATAGCTCA ATGTCTTAGT 
TATCTGGGAT TTGAGAACTT GGCGTGCACA TTGCCGCGCG CTAAGGTAAC CAGTGCCAGA 
GAGGACAAGA AGAAGGCAGA GTGCATGGTT GGAGTTGGAG CAGCTCGTTT CCAATTGCAG 
TTTATGAGTC CATACTTGGT AAGAGAGGAG AGGACAGATC CTGATCCAAG AGTACAACAC 
TTTATACCGG ACACATGGCA GCGTAAACTT CTTGATGTTG TTGACAATAA TGAGTCTGCG 
GTGATAGTTG CCCCTACATC TTCAGGAAAG ACTTACGCTT CGTACTACTG CATGCAGAAA 
GTTCTGAGTC AAGGCCATGA AGGTGTGGTT GTGTATGTTT CACCAACAAA GGCTCTTGTA 
AACCAGGTGG TAACCACTGT AATGAATCAG TTCAACAAAA ATTTGCCCAA TGGAGTGGCT 
CTGTGTGGTG TTTTCACAAG GGATTACCGT ACTGATGCAT TAAATTCTCA GATATTAGTA 
ACAGTGCCTC AGTGTCTTGA GATTCTTCTC TTGTCGCCAC ACCGTCAGGA ATGGGCTAAA 
AGGATTAAGT ATGTAATATT TGATGAGATT CATTGCCTGG GTGGAGAAAT AGGTGCAGAA 
GTATGGGAAC ATCTTCTAGT GATGATTCGC TGTCCATTTC TTGCTTTGTC TGCAACAATA 
AGTAACCCGG AACATCTAAC TGAATGGCTG CAATCAGTCA AATTTTACTG GCAAAAAAGA 
GATAAATTAA TAGAGGAAAA CTGGACTGAG AAAGGAGCAA AGGCAAAAGC CTATAAGAAG 
AAAGAAAAAA TGTCTTACAG AGTTCATCTG GTGATTTATA ACAAGAGATA CAATGATCTG 
GAAAAATATG TGTGCTCTGT GCAAGACTCC AATGTATCGT TTGAGCACTA TCACCCATGT 
GCTGCCTTAA CCACGGACCA CATGGAGAAA TATGGAATTC CAAATGACCT GTCCTGCTCC 
CCAAGGGAGA GTTTACAACT GTATGACACT ATGGTGGATG TGTGGCCTGA ATGGGCAAGG 
AGAGAGGAAC TCGATCCTGA AGAATACGCA CATTTCAAGG ACAGAATCAT CATAACAAAA 
AATGATACAA AAATATATGA GGAAGAACTT AAAAAAGAAT TAATTGACTG GATTAAGCAA 
GGAAACCAGG AAAAGGTTTC CCAACTATTG AAGTATCTTC AGCCACCAAA ATCATCTTGT 
AATGTGGACC GTGTCGCCTA CTTTCCAAAG CTTGTTGAAA AACTGAAAGA AGAGCAAAAG 
CTGCCAGCTC TTCTTTTTGC ATTTAACATT GGACTTGTGG AACATCTCGC ACAGGAAACA 
GCCATATTTC TAAAAAACAA GCAAGACAAT AAGACAAAAC TTCTGACTGA GAAAAAAAGC 
CAAAAACTTA TCACCAAAGC AAAAAGACTG GAAAAAGCAA TAGCAAAAAA TGAACCCTCA 
AAAGTAAACG ACGCTTTCTT AGCCAATGTT GCCAATCATA AGAGTATAGA GCAAGTACTT 
ATAAAGGACA AAGAAATCCC ACCAGACTGT ACTTATGCTG ATACCAGAGC TGTGAACAAT 
GAGTTACTCT GGGAGGTGTT CAGACAAACA AGTCGATCAA GAGATGCCAA GTGTCTGAGG 
TTTCTGGCAG AAAGAGGTGT GGGCTACCAT CATGCCTCTT TGGATGTTAA AGGAAGAAGA 
CAAGTGGAGA TGCTCTTCAG GATGGGATAC CTACGGGTTG TTACTGCAAC TGGATCTCTT 
GCCTTGGGAA TCAACATGCC GTGTAAATCT GTTGTGTTTG TTCAAGATTC ACTTTACCTG 
GATGCATTAA ATTACAGACA GATGTCAGGA CGTGCAGGAA GGCGAGGACA GGATGTACTG 
GGAAATGTAT ACTTTTTCAC CATTCCTATG CCTAAAGTGA AACGGCTTAT GAAATCAGAT 
GTCCCTAACT TAAAAGGACA GTTCCCTTTA GGCATCTCTT TTGTCCTACG ACTGATGTTA 
TTGGCTGCCA AAGCTGATCA CAAAGAAGAT GCCAGTGCTA AGGCTTTGTC AGTACTGAAG 
CACTCCCTGA TGGCGTTCAA AAAACCTTCT GAGACCAAAA TGTTGAAGCT CTACTTTCTG 
TTCTGCATAC ACTTCTTAAT CCATGAGGGC TATTTGGATC AACATTGCAA CCCTTTGGAC 
TTTACCGGGC TGGTTACACA TTTACATTAC CATGAACCAT CAAATTTTGT GTTTGTCAGT 
TTTCTGGAAA ATGGTTTATT TCACCGGCTG TGCCAGAGAA ACACTAAAGG TTCAAATAGG 
TTTCCACAAT CTGTGATGGA AACCCTGGTG CTGGTATTGG CTAACTTGTT TGGAAGAAGC 
TACCTTTTAC CATCCATGCT AGAATTAAAG GGAACATTTA AACAGTCAAA GGTCTTTCTG 
GAAGGTCTAC CAGATGATTT TGCTGCAGCT CTTGAAGACT ACAATTCCAA AATATCAGCA 
GTCTTTGGGC AGTATCTTCT TGCTGTTTCT CATCTGGCAA ATTATGAACA AGAATACAAG 
CTTCCTCTAT CACAAATCAA CTTCTCAGGG GAGGAATGTG AGGATTCTGA ACTTGTAAAC 
CACTTGATGA ACTGCACCAA AAGGAGAAGT GCCATAACCC CCTTTGCCTG CCTGTCTGGA 
AATACAGATC ATGACTTAAC TTTTGCAGCA CATTTGGACA GTATTTTGTT GCAAACTAGT 
CATGTGAGTG AAAAACACAT TCCTCGCCTG CATTTGGAGA ACACAGATGC TTGTGGTAGA 
AAACTGTTAC TAAATGCCTA TGCACTGGAT TTCTTCAAGC ACAGCTCCAT AGATGCACTG 
GAAACTGATA ATGGGTTTCA CAAAGGAGAT GCTTTTTACA AGTTACGAGA TTTTTCTTTA 
TGCATTGCAA CTATAAGAGT TTCTTTAAAG GAAATGTGTG AAGATGAGGA TGACCCCGTT 
GTGTTAGCTT TCGAGCAGCT GAATACCCTG TACAGAGAGA AACTGGAGAC TGCTTATCCC 
AGGCGCAGAA TCTTTTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx60 ( XM_031895462.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751322.1 Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X5, mRNA.

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CloneID	OXa49817
Clone ID Related Accession (Same CDS sequence)	XM_031895461.1 , XM_031895462.1
Accession Version	XM_031895462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX60 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AAGGGTCTCA TTTGGAAAAG ATCTCACTGG ACTCTCAAAC AGAGATTTCT 
GTATCACATG AAGCAGAACC AAAGACTAAA AATGAATCCC GAATGAAAAA TGATGACGCC 
CTTCGTGACT TGTTCACTTT TACAGTGCAG AAACTCTCAG GAGCTGTCTT TGCCAGCCTG 
CTTAATGATT ATGTGGAATC TGAATTCTTC TTGATTGATG GAGATTCCTT ACTGCTAACT 
TGTGTTTTGG ATCAGTCACT GAAGGCAGGG CAGCATCTGC ATTTCTTTTA CTTGGTAGAA 
TCCTACTTGC ATAAACTGAC CAACCGGGGA GCAAAATATG CTGTTGTGTT TTTTGAGGAT 
ATGGAGTTTT TCTACCATGA CAGAGTCGAC CTTATCTCTC TACGGACCCA ACTGAAACTG 
CACTTGGAAC ACAACACAGA CACAGTCATA TACACCTTTT CCAACTTTTT GTCTGCTCAG 
TGGAACACAT TTCTGATTGA TCACCATCCC TACTTTCTAA TGGTTTGTGA TGAAGGTGTT 
ACCCTGTCTC AAACATATTT CTTCAGTATG CTTATGCTAA GTGCCCTGGG GAAAAAAATA 
GACCTCGTAC TCACGTCAGG ACAAGAGTTC GATCAGCTCC GAGTGTATGG ATACCATATC 
AATGCCACCC ACCATCATAA CTTATATCCC ATAAAGAAAC AGAAGCAGTT TCTTCAAGAT 
TTGGTAGATC CATGTGAAAA GTTGTACAGA AAGCGTGCAC AGAAGCTGAA TTTCAGTAAC 
ACCATAAACG AGATTGAAAA GAGTATAACA TTACTTTTTA ACAATGGGGA TAAGGCTTTA 
GACATAAGAA GCATCATGTG TATTCTGTCC TGCTCTGCAG TGCTGACAAT ATATGGTAAA 
AAAGCAACAC ATGGAGAATG TCAGGCCTCA CAGGTACAGG ATGACAGTGA CCTGACCTTG 
GAAGAAGTGG CTGACCTATG CAGAATGTAC TGTCTGAGTG TGGCCATATT GCTGGTTTTG 
CCCCTTTCCC AGAGAATGAA ATCTAGGAAC ATCCATGCAG ATTGGAACAG CCCAGTATTA 
GCATGTTTAG ATTGGCAAAT AATATGTGAG CACATTATTC TGAAGAAGTT TTCCAACACC 
TCAAACTGGA AAATAAATTG GACACACATT GCTGATCTAA GTGATGGTTT GCTGTTGAAA 
AACATTGCAT ATTACTATGA AAAAGAGAAC AATACAGGCT CCAAGTTGAT ATTCGGGAAG 
GCAATAGAGG AGAAGTTTTC ATTTCTCTGG AACTCCATTT TGGAAATTAA TCCACATAGT 
GAACAAATAC AATCTTATGA CGTCAGAACA ACATCACGTC CTTTCCTTTC AAAGGCTTCC 
TCAATAATTG TGAGAGAGGA GAACATGCCA ACTATAGGTC TTCTACGAGC AAAATCTGAC 
ATTGTTGAAG AGTATGCTGG GGATTTTTTG GATGAATTAC CATTTTTAAG CAGTATTGAA 
CATAAAGAGA CCCCAGTCAC TCGAGGGAAA TATTTTGATG AACTTACACA CTGGCATTCT 
GGACGTCCAC TAAGTGATGA TTATGACAGA ACAAAGGGAG ATGAGGCAGC ATTGAAAGAT 
GAACGTGCTC TCAGAGATTA CCAAAAGCTG CAAACTCTTC ACCGTTTTAG CGGGCAATCT 
TTAGTCTCCT CATCATCCAA GACAATTGTA ATACAGAATG ATGACCCTGA AAAGAAGGGG 
GAGAAAGAAA AGGTTATTGT TAAGAAAAAG CAACCACAGA AGTCTAAAAA AGATCTAATC 
ATAGAGGAAA ACCAGAAAAG AATGAATGCC AAAGAGGAAA CTAAAGACGC TGAACAGTGG 
AAATTAGTGG CATCTTCAAT TGAAAAAGAA ATGAGAGCAA ATTTTTTTCC TGGCTTAAAA 
AAACTGGAAA GTTTCATAAA AAATACCCAA ACCCGCTCAG TGAAGCTTCT TGCAGAACTA 
TTAGGATTAA GAATTTCCTT TAAGGTGTGG GTCGAGCACT GCTGCACAGA CATGTCCTCA 
GAAGAGGAGG ACGATTTAAA TATTGCTGTG GAAATCATGA AGAAGATACA AACACTTCAT 
TATAAATACA AGGACATGCT GACTAATGAG GATCAACAAC TAATAGCTCA ATGTCTTAGT 
TATCTGGGAT TTGAGAACTT GGCGTGCACA TTGCCGCGCG CTAAGGTAAC CAGTGCCAGA 
GAGGACAAGA AGAAGGCAGA GTGCATGGTT GGAGTTGGAG CAGCTCGTTT CCAATTGCAG 
TTTATGAGTC CATACTTGGT AAGAGAGGAG AGGACAGATC CTGATCCAAG AGTACAACAC 
TTTATACCGG ACACATGGCA GCGTAAACTT CTTGATGTTG TTGACAATAA TGAGTCTGCG 
GTGATAGTTG CCCCTACATC TTCAGGAAAG ACTTACGCTT CGTACTACTG CATGCAGAAA 
GTTCTGAGTC AAGGCCATGA AGGTGTGGTT GTGTATGTTT CACCAACAAA GGCTCTTGTA 
AACCAGGTGG TAACCACTGT AATGAATCAG TTCAACAAAA ATTTGCCCAA TGGAGTGGCT 
CTGTGTGGTG TTTTCACAAG GGATTACCGT ACTGATGCAT TAAATTCTCA GATATTAGTA 
ACAGTGCCTC AGTGTCTTGA GATTCTTCTC TTGTCGCCAC ACCGTCAGGA ATGGGCTAAA 
AGGATTAAGT ATGTAATATT TGATGAGATT CATTGCCTGG GTGGAGAAAT AGGTGCAGAA 
GTATGGGAAC ATCTTCTAGT GATGATTCGC TGTCCATTTC TTGCTTTGTC TGCAACAATA 
AGTAACCCGG AACATCTAAC TGAATGGCTG CAATCAGTCA AATTTTACTG GCAAAAAAGA 
GATAAATTAA TAGAGGAAAA CTGGACTGAG AAAGGAGCAA AGGCAAAAGC CTATAAGAAG 
AAAGAAAAAA TGTCTTACAG AGTTCATCTG GTGATTTATA ACAAGAGATA CAATGATCTG 
GAAAAATATG TGTGCTCTGT GCAAGACTCC AATGTATCGT TTGAGCACTA TCACCCATGT 
GCTGCCTTAA CCACGGACCA CATGGAGAAA TATGGAATTC CAAATGACCT GTCCTGCTCC 
CCAAGGGAGA GTTTACAACT GTATGACACT ATGGTGGATG TGTGGCCTGA ATGGGCAAGG 
AGAGAGGAAC TCGATCCTGA AGAATACGCA CATTTCAAGG ACAGAATCAT CATAACAAAA 
AATGATACAA AAATATATGA GGAAGAACTT AAAAAAGAAT TAATTGACTG GATTAAGCAA 
GGAAACCAGG AAAAGGTTTC CCAACTATTG AAGTATCTTC AGCCACCAAA ATCATCTTGT 
AATGTGGACC GTGTCGCCTA CTTTCCAAAG CTTGTTGAAA AACTGAAAGA AGAGCAAAAG 
CTGCCAGCTC TTCTTTTTGC ATTTAACATT GGACTTGTGG AACATCTCGC ACAGGAAACA 
GCCATATTTC TAAAAAACAA GCAAGACAAT AAGACAAAAC TTCTGACTGA GAAAAAAAGC 
CAAAAACTTA TCACCAAAGC AAAAAGACTG GAAAAAGCAA TAGCAAAAAA TGAACCCTCA 
AAAGTAAACG ACGCTTTCTT AGCCAATGTT GCCAATCATA AGAGTATAGA GCAAGTACTT 
ATAAAGGACA AAGAAATCCC ACCAGACTGT ACTTATGCTG ATACCAGAGC TGTGAACAAT 
GAGTTACTCT GGGAGGTGTT CAGACAAACA AGTCGATCAA GAGATGCCAA GTGTCTGAGG 
TTTCTGGCAG AAAGAGGTGT GGGCTACCAT CATGCCTCTT TGGATGTTAA AGGAAGAAGA 
CAAGTGGAGA TGCTCTTCAG GATGGGATAC CTACGGGTTG TTACTGCAAC TGGATCTCTT 
GCCTTGGGAA TCAACATGCC GTGTAAATCT GTTGTGTTTG TTCAAGATTC ACTTTACCTG 
GATGCATTAA ATTACAGACA GATGTCAGGA CGTGCAGGAA GGCGAGGACA GGATGTACTG 
GGAAATGTAT ACTTTTTCAC CATTCCTATG CCTAAAGTGA AACGGCTTAT GAAATCAGAT 
GTCCCTAACT TAAAAGGACA GTTCCCTTTA GGCATCTCTT TTGTCCTACG ACTGATGTTA 
TTGGCTGCCA AAGCTGATCA CAAAGAAGAT GCCAGTGCTA AGGCTTTGTC AGTACTGAAG 
CACTCCCTGA TGGCGTTCAA AAAACCTTCT GAGACCAAAA TGTTGAAGCT CTACTTTCTG 
TTCTGCATAC ACTTCTTAAT CCATGAGGGC TATTTGGATC AACATTGCAA CCCTTTGGAC 
TTTACCGGGC TGGTTACACA TTTACATTAC CATGAACCAT CAAATTTTGT GTTTGTCAGT 
TTTCTGGAAA ATGGTTTATT TCACCGGCTG TGCCAGAGAA ACACTAAAGG TTCAAATAGG 
TTTCCACAAT CTGTGATGGA AACCCTGGTG CTGGTATTGG CTAACTTGTT TGGAAGAAGC 
TACCTTTTAC CATCCATGCT AGAATTAAAG GGAACATTTA AACAGTCAAA GGTCTTTCTG 
GAAGGTCTAC CAGATGATTT TGCTGCAGCT CTTGAAGACT ACAATTCCAA AATATCAGCA 
GTCTTTGGGC AGTATCTTCT TGCTGTTTCT CATCTGGCAA ATTATGAACA AGAATACAAG 
CTTCCTCTAT CACAAATCAA CTTCTCAGGG GAGGAATGTG AGGATTCTGA ACTTGTAAAC 
CACTTGATGA ACTGCACCAA AAGGAGAAGT GCCATAACCC CCTTTGCCTG CCTGTCTGGA 
AATACAGATC ATGACTTAAC TTTTGCAGCA CATTTGGACA GTATTTTGTT GCAAACTAGT 
CATGTGAGTG AAAAACACAT TCCTCGCCTG CATTTGGAGA ACACAGATGC TTGTGGTAGA 
AAACTGTTAC TAAATGCCTA TGCACTGGAT TTCTTCAAGC ACAGCTCCAT AGATGCACTG 
GAAACTGATA ATGGGTTTCA CAAAGGAGAT GCTTTTTACA AGTTACGAGA TTTTTCTTTA 
TGCATTGCAA CTATAAGAGT TTCTTTAAAG GAAATGTGTG AAGATGAGGA TGACCCCGTT 
GTGTTAGCTT TCGAGCAGCT GAATACCCTG TACAGAGAGA AACTGGAGAC TGCTTATCCC 
AGGCGCAGAA TCTTTTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751322.1
CDS	555..5738
Translation	MATQGSHLEKISLDSQTEISVSHEAEPKTKNESRMKNDDALRDLFTFTVQKLSGAVFASLLNDYVESEFFLIDGDSLLLTCVLDQSLKAGQHLHFFYLVESYLHKLTNRGAKYAVVFFEDMEFFYHDRVDLISLRTQLKLHLEHNTDTVIYTFSNFLSAQWNTFLIDHHPYFLMVCDEGVTLSQTYFFSMLMLSALGKKIDLVLTSGQEFDQLRVYGYHINATHHHNLYPIKKQKQFLQDLVDPCEKLYRKRAQKLNFSNTINEIEKSITLLFNNGDKALDIRSIMCILSCSAVLTIYGKKATHGECQASQVQDDSDLTLEEVADLCRMYCLSVAILLVLPLSQRMKSRNIHADWNSPVLACLDWQIICEHIILKKFSNTSNWKINWTHIADLSDGLLLKNIAYYYEKENNTGSKLIFGKAIEEKFSFLWNSILEINPHSEQIQSYDVRTTSRPFLSKASSIIVREENMPTIGLLRAKSDIVEEYAGDFLDELPFLSSIEHKETPVTRGKYFDELTHWHSGRPLSDDYDRTKGDEAALKDERALRDYQKLQTLHRFSGQSLVSSSSKTIVIQNDDPEKKGEKEKVIVKKKQPQKSKKDLIIEENQKRMNAKEETKDAEQWKLVASSIEKEMRANFFPGLKKLESFIKNTQTRSVKLLAELLGLRISFKVWVEHCCTDMSSEEEDDLNIAVEIMKKIQTLHYKYKDMLTNEDQQLIAQCLSYLGFENLACTLPRAKVTSAREDKKKAECMVGVGAARFQLQFMSPYLVREERTDPDPRVQHFIPDTWQRKLLDVVDNNESAVIVAPTSSGKTYASYYCMQKVLSQGHEGVVVYVSPTKALVNQVVTTVMNQFNKNLPNGVALCGVFTRDYRTDALNSQILVTVPQCLEILLLSPHRQEWAKRIKYVIFDEIHCLGGEIGAEVWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKFYWQKRDKLIEENWTEKGAKAKAYKKKEKMSYRVHLVIYNKRYNDLEKYVCSVQDSNVSFEHYHPCAALTTDHMEKYGIPNDLSCSPRESLQLYDTMVDVWPEWARREELDPEEYAHFKDRIIITKNDTKIYEEELKKELIDWIKQGNQEKVSQLLKYLQPPKSSCNVDRVAYFPKLVEKLKEEQKLPALLFAFNIGLVEHLAQETAIFLKNKQDNKTKLLTEKKSQKLITKAKRLEKAIAKNEPSKVNDAFLANVANHKSIEQVLIKDKEIPPDCTYADTRAVNNELLWEVFRQTSRSRDAKCLRFLAERGVGYHHASLDVKGRRQVEMLFRMGYLRVVTATGSLALGINMPCKSVVFVQDSLYLDALNYRQMSGRAGRRGQDVLGNVYFFTIPMPKVKRLMKSDVPNLKGQFPLGISFVLRLMLLAAKADHKEDASAKALSVLKHSLMAFKKPSETKMLKLYFLFCIHFLIHEGYLDQHCNPLDFTGLVTHLHYHEPSNFVFVSFLENGLFHRLCQRNTKGSNRFPQSVMETLVLVLANLFGRSYLLPSMLELKGTFKQSKVFLEGLPDDFAAALEDYNSKISAVFGQYLLAVSHLANYEQEYKLPLSQINFSGEECEDSELVNHLMNCTKRRSAITPFACLSGNTDHDLTFAAHLDSILLQTSHVSEKHIPRLHLENTDACGRKLLLNAYALDFFKHSSIDALETDNGFHKGDAFYKLRDFSLCIATIRVSLKEMCEDEDDPVVLAFEQLNTLYREKLETAYPRRRIFYA

Target ORF information:

RefSeq Version	XM_031895462.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEXD/H-box helicase 60 (ddx60), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895462.1

ORF Insert Sequence:

ATGGCCACAC AAGGGTCTCA TTTGGAAAAG ATCTCACTGG ACTCTCAAAC AGAGATTTCT 
GTATCACATG AAGCAGAACC AAAGACTAAA AATGAATCCC GAATGAAAAA TGATGACGCC 
CTTCGTGACT TGTTCACTTT TACAGTGCAG AAACTCTCAG GAGCTGTCTT TGCCAGCCTG 
CTTAATGATT ATGTGGAATC TGAATTCTTC TTGATTGATG GAGATTCCTT ACTGCTAACT 
TGTGTTTTGG ATCAGTCACT GAAGGCAGGG CAGCATCTGC ATTTCTTTTA CTTGGTAGAA 
TCCTACTTGC ATAAACTGAC CAACCGGGGA GCAAAATATG CTGTTGTGTT TTTTGAGGAT 
ATGGAGTTTT TCTACCATGA CAGAGTCGAC CTTATCTCTC TACGGACCCA ACTGAAACTG 
CACTTGGAAC ACAACACAGA CACAGTCATA TACACCTTTT CCAACTTTTT GTCTGCTCAG 
TGGAACACAT TTCTGATTGA TCACCATCCC TACTTTCTAA TGGTTTGTGA TGAAGGTGTT 
ACCCTGTCTC AAACATATTT CTTCAGTATG CTTATGCTAA GTGCCCTGGG GAAAAAAATA 
GACCTCGTAC TCACGTCAGG ACAAGAGTTC GATCAGCTCC GAGTGTATGG ATACCATATC 
AATGCCACCC ACCATCATAA CTTATATCCC ATAAAGAAAC AGAAGCAGTT TCTTCAAGAT 
TTGGTAGATC CATGTGAAAA GTTGTACAGA AAGCGTGCAC AGAAGCTGAA TTTCAGTAAC 
ACCATAAACG AGATTGAAAA GAGTATAACA TTACTTTTTA ACAATGGGGA TAAGGCTTTA 
GACATAAGAA GCATCATGTG TATTCTGTCC TGCTCTGCAG TGCTGACAAT ATATGGTAAA 
AAAGCAACAC ATGGAGAATG TCAGGCCTCA CAGGTACAGG ATGACAGTGA CCTGACCTTG 
GAAGAAGTGG CTGACCTATG CAGAATGTAC TGTCTGAGTG TGGCCATATT GCTGGTTTTG 
CCCCTTTCCC AGAGAATGAA ATCTAGGAAC ATCCATGCAG ATTGGAACAG CCCAGTATTA 
GCATGTTTAG ATTGGCAAAT AATATGTGAG CACATTATTC TGAAGAAGTT TTCCAACACC 
TCAAACTGGA AAATAAATTG GACACACATT GCTGATCTAA GTGATGGTTT GCTGTTGAAA 
AACATTGCAT ATTACTATGA AAAAGAGAAC AATACAGGCT CCAAGTTGAT ATTCGGGAAG 
GCAATAGAGG AGAAGTTTTC ATTTCTCTGG AACTCCATTT TGGAAATTAA TCCACATAGT 
GAACAAATAC AATCTTATGA CGTCAGAACA ACATCACGTC CTTTCCTTTC AAAGGCTTCC 
TCAATAATTG TGAGAGAGGA GAACATGCCA ACTATAGGTC TTCTACGAGC AAAATCTGAC 
ATTGTTGAAG AGTATGCTGG GGATTTTTTG GATGAATTAC CATTTTTAAG CAGTATTGAA 
CATAAAGAGA CCCCAGTCAC TCGAGGGAAA TATTTTGATG AACTTACACA CTGGCATTCT 
GGACGTCCAC TAAGTGATGA TTATGACAGA ACAAAGGGAG ATGAGGCAGC ATTGAAAGAT 
GAACGTGCTC TCAGAGATTA CCAAAAGCTG CAAACTCTTC ACCGTTTTAG CGGGCAATCT 
TTAGTCTCCT CATCATCCAA GACAATTGTA ATACAGAATG ATGACCCTGA AAAGAAGGGG 
GAGAAAGAAA AGGTTATTGT TAAGAAAAAG CAACCACAGA AGTCTAAAAA AGATCTAATC 
ATAGAGGAAA ACCAGAAAAG AATGAATGCC AAAGAGGAAA CTAAAGACGC TGAACAGTGG 
AAATTAGTGG CATCTTCAAT TGAAAAAGAA ATGAGAGCAA ATTTTTTTCC TGGCTTAAAA 
AAACTGGAAA GTTTCATAAA AAATACCCAA ACCCGCTCAG TGAAGCTTCT TGCAGAACTA 
TTAGGATTAA GAATTTCCTT TAAGGTGTGG GTCGAGCACT GCTGCACAGA CATGTCCTCA 
GAAGAGGAGG ACGATTTAAA TATTGCTGTG GAAATCATGA AGAAGATACA AACACTTCAT 
TATAAATACA AGGACATGCT GACTAATGAG GATCAACAAC TAATAGCTCA ATGTCTTAGT 
TATCTGGGAT TTGAGAACTT GGCGTGCACA TTGCCGCGCG CTAAGGTAAC CAGTGCCAGA 
GAGGACAAGA AGAAGGCAGA GTGCATGGTT GGAGTTGGAG CAGCTCGTTT CCAATTGCAG 
TTTATGAGTC CATACTTGGT AAGAGAGGAG AGGACAGATC CTGATCCAAG AGTACAACAC 
TTTATACCGG ACACATGGCA GCGTAAACTT CTTGATGTTG TTGACAATAA TGAGTCTGCG 
GTGATAGTTG CCCCTACATC TTCAGGAAAG ACTTACGCTT CGTACTACTG CATGCAGAAA 
GTTCTGAGTC AAGGCCATGA AGGTGTGGTT GTGTATGTTT CACCAACAAA GGCTCTTGTA 
AACCAGGTGG TAACCACTGT AATGAATCAG TTCAACAAAA ATTTGCCCAA TGGAGTGGCT 
CTGTGTGGTG TTTTCACAAG GGATTACCGT ACTGATGCAT TAAATTCTCA GATATTAGTA 
ACAGTGCCTC AGTGTCTTGA GATTCTTCTC TTGTCGCCAC ACCGTCAGGA ATGGGCTAAA 
AGGATTAAGT ATGTAATATT TGATGAGATT CATTGCCTGG GTGGAGAAAT AGGTGCAGAA 
GTATGGGAAC ATCTTCTAGT GATGATTCGC TGTCCATTTC TTGCTTTGTC TGCAACAATA 
AGTAACCCGG AACATCTAAC TGAATGGCTG CAATCAGTCA AATTTTACTG GCAAAAAAGA 
GATAAATTAA TAGAGGAAAA CTGGACTGAG AAAGGAGCAA AGGCAAAAGC CTATAAGAAG 
AAAGAAAAAA TGTCTTACAG AGTTCATCTG GTGATTTATA ACAAGAGATA CAATGATCTG 
GAAAAATATG TGTGCTCTGT GCAAGACTCC AATGTATCGT TTGAGCACTA TCACCCATGT 
GCTGCCTTAA CCACGGACCA CATGGAGAAA TATGGAATTC CAAATGACCT GTCCTGCTCC 
CCAAGGGAGA GTTTACAACT GTATGACACT ATGGTGGATG TGTGGCCTGA ATGGGCAAGG 
AGAGAGGAAC TCGATCCTGA AGAATACGCA CATTTCAAGG ACAGAATCAT CATAACAAAA 
AATGATACAA AAATATATGA GGAAGAACTT AAAAAAGAAT TAATTGACTG GATTAAGCAA 
GGAAACCAGG AAAAGGTTTC CCAACTATTG AAGTATCTTC AGCCACCAAA ATCATCTTGT 
AATGTGGACC GTGTCGCCTA CTTTCCAAAG CTTGTTGAAA AACTGAAAGA AGAGCAAAAG 
CTGCCAGCTC TTCTTTTTGC ATTTAACATT GGACTTGTGG AACATCTCGC ACAGGAAACA 
GCCATATTTC TAAAAAACAA GCAAGACAAT AAGACAAAAC TTCTGACTGA GAAAAAAAGC 
CAAAAACTTA TCACCAAAGC AAAAAGACTG GAAAAAGCAA TAGCAAAAAA TGAACCCTCA 
AAAGTAAACG ACGCTTTCTT AGCCAATGTT GCCAATCATA AGAGTATAGA GCAAGTACTT 
ATAAAGGACA AAGAAATCCC ACCAGACTGT ACTTATGCTG ATACCAGAGC TGTGAACAAT 
GAGTTACTCT GGGAGGTGTT CAGACAAACA AGTCGATCAA GAGATGCCAA GTGTCTGAGG 
TTTCTGGCAG AAAGAGGTGT GGGCTACCAT CATGCCTCTT TGGATGTTAA AGGAAGAAGA 
CAAGTGGAGA TGCTCTTCAG GATGGGATAC CTACGGGTTG TTACTGCAAC TGGATCTCTT 
GCCTTGGGAA TCAACATGCC GTGTAAATCT GTTGTGTTTG TTCAAGATTC ACTTTACCTG 
GATGCATTAA ATTACAGACA GATGTCAGGA CGTGCAGGAA GGCGAGGACA GGATGTACTG 
GGAAATGTAT ACTTTTTCAC CATTCCTATG CCTAAAGTGA AACGGCTTAT GAAATCAGAT 
GTCCCTAACT TAAAAGGACA GTTCCCTTTA GGCATCTCTT TTGTCCTACG ACTGATGTTA 
TTGGCTGCCA AAGCTGATCA CAAAGAAGAT GCCAGTGCTA AGGCTTTGTC AGTACTGAAG 
CACTCCCTGA TGGCGTTCAA AAAACCTTCT GAGACCAAAA TGTTGAAGCT CTACTTTCTG 
TTCTGCATAC ACTTCTTAAT CCATGAGGGC TATTTGGATC AACATTGCAA CCCTTTGGAC 
TTTACCGGGC TGGTTACACA TTTACATTAC CATGAACCAT CAAATTTTGT GTTTGTCAGT 
TTTCTGGAAA ATGGTTTATT TCACCGGCTG TGCCAGAGAA ACACTAAAGG TTCAAATAGG 
TTTCCACAAT CTGTGATGGA AACCCTGGTG CTGGTATTGG CTAACTTGTT TGGAAGAAGC 
TACCTTTTAC CATCCATGCT AGAATTAAAG GGAACATTTA AACAGTCAAA GGTCTTTCTG 
GAAGGTCTAC CAGATGATTT TGCTGCAGCT CTTGAAGACT ACAATTCCAA AATATCAGCA 
GTCTTTGGGC AGTATCTTCT TGCTGTTTCT CATCTGGCAA ATTATGAACA AGAATACAAG 
CTTCCTCTAT CACAAATCAA CTTCTCAGGG GAGGAATGTG AGGATTCTGA ACTTGTAAAC 
CACTTGATGA ACTGCACCAA AAGGAGAAGT GCCATAACCC CCTTTGCCTG CCTGTCTGGA 
AATACAGATC ATGACTTAAC TTTTGCAGCA CATTTGGACA GTATTTTGTT GCAAACTAGT 
CATGTGAGTG AAAAACACAT TCCTCGCCTG CATTTGGAGA ACACAGATGC TTGTGGTAGA 
AAACTGTTAC TAAATGCCTA TGCACTGGAT TTCTTCAAGC ACAGCTCCAT AGATGCACTG 
GAAACTGATA ATGGGTTTCA CAAAGGAGAT GCTTTTTACA AGTTACGAGA TTTTTCTTTA 
TGCATTGCAA CTATAAGAGT TTCTTTAAAG GAAATGTGTG AAGATGAGGA TGACCCCGTT 
GTGTTAGCTT TCGAGCAGCT GAATACCCTG TACAGAGAGA AACTGGAGAC TGCTTATCCC 
AGGCGCAGAA TCTTTTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

ddx60 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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