ephb3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ephb3
Entrez Gene ID	100489199
Full Name	EPH receptor B3
Synonyms	tck
General protein information	Preferred Names EPH receptor B3 Names ephrin type-B receptor 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935148.4	XP_002935194.1	ephrin type-B receptor 3
XM_002935148.5	XP_002935194.1	ephrin type-B receptor 3

Pathways from BioSystems

Homologies

	EPHB3	XP_001097522.2
Rattus norvegicus (Norway rat)	Ephb3	NP_001099338.1
Gallus gallus (chicken)	EPHB3	XP_422762.4
Homo sapiens (human)	EPHB3	NP_004434.2
Pan troglodytes (chimpanzee)	EPHB3	XP_003310198.1
Bos taurus (cattle)	EPHB3	NP_001179725.1
Danio rerio (zebrafish)	LOC555957	XP_683712.6
Canis lupus familiaris (dog)	EPHB3	XP_545232.3
Mus musculus (house mouse)	Ephb3	NP_034273.1
Xenopus tropicalis (tropical clawed frog)	LOC100489199	XP_002935194.1

Gene Ontology

The following ephb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ephb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa10215	XM_002935148.4 Latest version!	Xenopus tropicalis EPH receptor B3 (ephb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa58160	XM_002935148.5 Latest version!	Xenopus tropicalis EPH receptor B3 (ephb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ephb3 ( XM_002935148.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935194.1 Xenopus tropicalis EPH receptor B3 (ephb3), mRNA.

Price & Availability↑

CloneID	OXa10215
Clone ID Related Accession (Same CDS sequence)	XM_002935148.4
Accession Version	XM_002935148.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephrin type-B receptor 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG CTGTGTTCGT GATGCTGGCG CTGTCGGCAG TGCAAGGGCT GGAAGAGACT 
CTGATGGACA CAAAATGGAC AACATCAGAA TTAGCATGGG TTGCATACCC AGATTCAGGG 
TGGGAGGAAG TAAGTGGATA CGATGAAGCC TCAAACCCAA TCCGGACGTA TCAGGTTTGT 
AATGTACGGG ATGGTAATCA GAATAATTGG CTACGTACGC AGTTTATTCC ACGGCAAGAT 
GTTCAGCGCG TGTATGTGGA GTTGAAGTTT ACTGTCAGAG ACTGTAACAG CCTGCCGAAT 
ATCCCAGGAT CTTGCAAAGA GACCTTTAAT TTCTTCTACT ATGAATCGGA CTCTGACTCT 
GCTTCTGCTG ACAGCCCATT CTGGATGGAA AACCCATACA TGAAGGTGGA TACCATAGCT 
CCAGATGAGA GCTTTTCACG ACGGGATTCT GGGCGTGTCA ACACAAAGAT ACGTAGCTTT 
GGACCTTTAT CCCGTACAGG TTTCTACTTG GCTTTTCAGG ACCTGGGTGC CTGTGTATCC 
CTAATTTCAG TTCGTGTCTT CTTTAAAAAG TGTCCTCGAA CCATTGCTGG ATTTGCCACT 
TTTCCCGAGA CAGTTACAGG GGCTGAAGCA ACATCACTTG TTATTGCACC AGGTACATGT 
GTGCCGAATG CCCTAGAAGT TTCTGTGCCT CTCAAGCTGT ACTGCAATGG AGATGGAGAA 
TGGATGGTGC CTGTGGGTGC CTGTACATGC GCATCTGGGT TTGAAGCAGC TGGGAAGGAC 
ACTCAATGCC AAGCATGCAA ACGTGGAACA TACAAGTCAA AGCAGGGAGA GGGAAGCTGT 
GTGCCTTGCC CCGTTAACAG CAGAGCCATT TCTTCAGCAG CCACTATCTG TTCCTGTCAG 
AATGGATACT ACCGTGCTGA CGGAGAGAGT GCTGAGACTG CGTGTACCTC TGTGCCCTCG 
GCACCACGAC ATGTTATCTC CAATGTAAAT GAAACCTCTG TGGTCCTTGA ATGGGCAGAG 
CCTGGTCATT TAGGCGGTCG GGATGACATT CTATACAATG TGATCTGTAA GAAATGCTTG 
GAGCGACTGT GTTCTCGCTG TGATGATAAT GTTCAGTTCT GGCCACGGCA GCTTGGTGTC 
ACCCAGAGGC GAGTCTCAGT CAGTCATCTC CAGGCCCACA CCAAGTATAG CTTTGAAATC 
CAGGCGGTCA ACGGAGTGTC TGGCAAAAGT CCACACATCC CCAACTACTA TACTGTGAAT 
ATTACAACTA ACCAGGCAGC CCCTTCAGCT GTGCCAATGG TGCAGTCTCA TGGAAGTTCG 
GCAAACTCTC TAACTCTGTC TTGGGCACCT CCAGAGAGCC CCAATGGCAT CATATTAGAC 
TATGAAATAA AATACTATCC CAAAGGCCAA AGCGGTGCAG GTAACACAGT GACCAGCCAG 
CGCACAACTG TGCGAATGGA AGCAATGACC CCAGATACAG TGTATGTTGT TCAGGTTCGA 
GCCCGTACTG TGGCTGGATA TGGAGCCTAT AGTGAACCTA GAGAGTTCCA GACAATCTCT 
GAAGATGGTG ACCGGAGCAG TCTGCAGGAA CAAGTCCCGA TGGTTGTTGG CTCTGTAACA 
GCCGGACTCA TTTTTATCAT TGCAGTGGTC ATTATAGTAA TAGTTTGTTT CAGCCGCAAG 
CAAAGGAATG ATGCTGAATC TGAATACACT GAAAAGCTGC AGCAGTACAT GGTGCCTGGC 
ATGAAGCTTT ACATTGATCC ATTTACGTAT GAGGATCCTA ATGAAGCTGT GCGAGACTTT 
GCTAAAGAAA TTGACATTTC CTGTGTGAAG ATTGAAGAAG TGATTGGTGC AGGTGAATTT 
GGGGAAGTGT GCCGGGGGAG ACTAAAGCAA GCAGGTCGCA GAGAACAGCT TGTAGCTATT 
AAGACATTGA AGGCAGGTTA CACAGAACAA CAAAGAAGAG ACTTTCTCGG TGAGGCCAGC 
ATCATGGGAC AGTTTGATCA TCCAAACATC ATTCGACTGG AAGGAGTTGT GACAAGGAGC 
CGGCCTGTCA TGATCTTGAC TGAATTTATG GAGAATGGAG CCCTTGACTC ATTTCTAAGG 
ATGAACGATG GACAGTTCAC AGTTATCCAG CTGGTTGGGA TACTCCGTGG CATTGCATCT 
GGAATGAAAT ATTTGTCTGA GATGAACTAT GTACATCGGG ATCTTGCTGC AAGGAACATC 
CTGGTGAACA GTAACCTTGT CTGCAAAGTG TCTGATTTTG GTCTGTCTAG ATTTTTGGAA 
AACAGTCGCT CTGATCCAAC GTACACAAGT TCATTGGGAG GAAAGATTCC AATTCGTTGG 
ACTGCTCCAG AGGCTATCTC ATACAGAAAG TTCACTTCTG CAAGCGATGT CTGGAGTTAT 
GGCATTGTCA TGTGGGAGGT TATGTCTTAC GGAGAGCGGC CATATTGGGA CATGTCAAAT 
CAAGATGTAA TTAATGCAAT TGAGCAAGAC TATCGGCTGC CACCTCCTAT GGATTGTCCT 
GCTGCTCTAC ACCAGCTCAT GCTGGACTGC TGGCTCCGTG ACCGCAACTT AAGACCCAAA 
TTCTCACAGA TCGTTAGTTC ACTTGACAAG CTAATACGTA ATGCAGCAAG TTTAAAGGTG 
ACCAGCCCAG CACAGGCAGG AGTATCTCAG CAGCTTCTAG ATCGCACTGT TCCTGATTAC 
ACTACTTTTC CCACTGTGAG CGATTGGTTG GAAGCCATTA AGATGGGGCA GTATCAAGAG 
AATTTTCTAA GCGCTGGCTT CACTTCTTTC CACTTGGTAG CACAAATGAC AGCTGAGGAC 
TTGCTCCGTA TCGGGGTGAC GCTGGCTGGT CACCAGAAAA AACTTCTCAA TAGTGTTCAG 
GACATGAGAC TGCAAATGAG TCAGACACTT CCTGTCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935194.1
CDS	274..3198
Translation	MVPAVFVMLALSAVQGLEETLMDTKWTTSELAWVAYPDSGWEEVSGYDEASNPIRTYQVCNVRDGNQNNWLRTQFIPRQDVQRVYVELKFTVRDCNSLPNIPGSCKETFNFFYYESDSDSASADSPFWMENPYMKVDTIAPDESFSRRDSGRVNTKIRSFGPLSRTGFYLAFQDLGACVSLISVRVFFKKCPRTIAGFATFPETVTGAEATSLVIAPGTCVPNALEVSVPLKLYCNGDGEWMVPVGACTCASGFEAAGKDTQCQACKRGTYKSKQGEGSCVPCPVNSRAISSAATICSCQNGYYRADGESAETACTSVPSAPRHVISNVNETSVVLEWAEPGHLGGRDDILYNVICKKCLERLCSRCDDNVQFWPRQLGVTQRRVSVSHLQAHTKYSFEIQAVNGVSGKSPHIPNYYTVNITTNQAAPSAVPMVQSHGSSANSLTLSWAPPESPNGIILDYEIKYYPKGQSGAGNTVTSQRTTVRMEAMTPDTVYVVQVRARTVAGYGAYSEPREFQTISEDGDRSSLQEQVPMVVGSVTAGLIFIIAVVIIVIVCFSRKQRNDAESEYTEKLQQYMVPGMKLYIDPFTYEDPNEAVRDFAKEIDISCVKIEEVIGAGEFGEVCRGRLKQAGRREQLVAIKTLKAGYTEQQRRDFLGEASIMGQFDHPNIIRLEGVVTRSRPVMILTEFMENGALDSFLRMNDGQFTVIQLVGILRGIASGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLENSRSDPTYTSSLGGKIPIRWTAPEAISYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWLRDRNLRPKFSQIVSSLDKLIRNAASLKVTSPAQAGVSQQLLDRTVPDYTTFPTVSDWLEAIKMGQYQENFLSAGFTSFHLVAQMTAEDLLRIGVTLAGHQKKLLNSVQDMRLQMSQTLPVQV

Target ORF information:

RefSeq Version	XM_002935148.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EPH receptor B3 (ephb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935148.4

ORF Insert Sequence:

ATGGTCCCGG CTGTGTTCGT GATGCTGGCG CTGTCGGCAG TGCAAGGGCT GGAAGAGACT 
CTGATGGACA CAAAATGGAC AACATCAGAA TTAGCATGGG TTGCATACCC AGATTCAGGG 
TGGGAGGAAG TAAGTGGATA CGATGAAGCC TCAAACCCAA TCCGGACGTA TCAGGTTTGT 
AATGTACGGG ATGGTAATCA GAATAATTGG CTACGTACGC AGTTTATTCC ACGGCAAGAT 
GTTCAGCGCG TGTATGTGGA GTTGAAGTTT ACTGTCAGAG ACTGTAACAG CCTGCCGAAT 
ATCCCAGGAT CTTGCAAAGA GACCTTTAAT TTCTTCTACT ATGAATCGGA CTCTGACTCT 
GCTTCTGCTG ACAGCCCATT CTGGATGGAA AACCCATACA TGAAGGTGGA TACCATAGCT 
CCAGATGAGA GCTTTTCACG ACGGGATTCT GGGCGTGTCA ACACAAAGAT ACGTAGCTTT 
GGACCTTTAT CCCGTACAGG TTTCTACTTG GCTTTTCAGG ACCTGGGTGC CTGTGTATCC 
CTAATTTCAG TTCGTGTCTT CTTTAAAAAG TGTCCTCGAA CCATTGCTGG ATTTGCCACT 
TTTCCCGAGA CAGTTACAGG GGCTGAAGCA ACATCACTTG TTATTGCACC AGGTACATGT 
GTGCCGAATG CCCTAGAAGT TTCTGTGCCT CTCAAGCTGT ACTGCAATGG AGATGGAGAA 
TGGATGGTGC CTGTGGGTGC CTGTACATGC GCATCTGGGT TTGAAGCAGC TGGGAAGGAC 
ACTCAATGCC AAGCATGCAA ACGTGGAACA TACAAGTCAA AGCAGGGAGA GGGAAGCTGT 
GTGCCTTGCC CCGTTAACAG CAGAGCCATT TCTTCAGCAG CCACTATCTG TTCCTGTCAG 
AATGGATACT ACCGTGCTGA CGGAGAGAGT GCTGAGACTG CGTGTACCTC TGTGCCCTCG 
GCACCACGAC ATGTTATCTC CAATGTAAAT GAAACCTCTG TGGTCCTTGA ATGGGCAGAG 
CCTGGTCATT TAGGCGGTCG GGATGACATT CTATACAATG TGATCTGTAA GAAATGCTTG 
GAGCGACTGT GTTCTCGCTG TGATGATAAT GTTCAGTTCT GGCCACGGCA GCTTGGTGTC 
ACCCAGAGGC GAGTCTCAGT CAGTCATCTC CAGGCCCACA CCAAGTATAG CTTTGAAATC 
CAGGCGGTCA ACGGAGTGTC TGGCAAAAGT CCACACATCC CCAACTACTA TACTGTGAAT 
ATTACAACTA ACCAGGCAGC CCCTTCAGCT GTGCCAATGG TGCAGTCTCA TGGAAGTTCG 
GCAAACTCTC TAACTCTGTC TTGGGCACCT CCAGAGAGCC CCAATGGCAT CATATTAGAC 
TATGAAATAA AATACTATCC CAAAGGCCAA AGCGGTGCAG GTAACACAGT GACCAGCCAG 
CGCACAACTG TGCGAATGGA AGCAATGACC CCAGATACAG TGTATGTTGT TCAGGTTCGA 
GCCCGTACTG TGGCTGGATA TGGAGCCTAT AGTGAACCTA GAGAGTTCCA GACAATCTCT 
GAAGATGGTG ACCGGAGCAG TCTGCAGGAA CAAGTCCCGA TGGTTGTTGG CTCTGTAACA 
GCCGGACTCA TTTTTATCAT TGCAGTGGTC ATTATAGTAA TAGTTTGTTT CAGCCGCAAG 
CAAAGGAATG ATGCTGAATC TGAATACACT GAAAAGCTGC AGCAGTACAT GGTGCCTGGC 
ATGAAGCTTT ACATTGATCC ATTTACGTAT GAGGATCCTA ATGAAGCTGT GCGAGACTTT 
GCTAAAGAAA TTGACATTTC CTGTGTGAAG ATTGAAGAAG TGATTGGTGC AGGTGAATTT 
GGGGAAGTGT GCCGGGGGAG ACTAAAGCAA GCAGGTCGCA GAGAACAGCT TGTAGCTATT 
AAGACATTGA AGGCAGGTTA CACAGAACAA CAAAGAAGAG ACTTTCTCGG TGAGGCCAGC 
ATCATGGGAC AGTTTGATCA TCCAAACATC ATTCGACTGG AAGGAGTTGT GACAAGGAGC 
CGGCCTGTCA TGATCTTGAC TGAATTTATG GAGAATGGAG CCCTTGACTC ATTTCTAAGG 
ATGAACGATG GACAGTTCAC AGTTATCCAG CTGGTTGGGA TACTCCGTGG CATTGCATCT 
GGAATGAAAT ATTTGTCTGA GATGAACTAT GTACATCGGG ATCTTGCTGC AAGGAACATC 
CTGGTGAACA GTAACCTTGT CTGCAAAGTG TCTGATTTTG GTCTGTCTAG ATTTTTGGAA 
AACAGTCGCT CTGATCCAAC GTACACAAGT TCATTGGGAG GAAAGATTCC AATTCGTTGG 
ACTGCTCCAG AGGCTATCTC ATACAGAAAG TTCACTTCTG CAAGCGATGT CTGGAGTTAT 
GGCATTGTCA TGTGGGAGGT TATGTCTTAC GGAGAGCGGC CATATTGGGA CATGTCAAAT 
CAAGATGTAA TTAATGCAAT TGAGCAAGAC TATCGGCTGC CACCTCCTAT GGATTGTCCT 
GCTGCTCTAC ACCAGCTCAT GCTGGACTGC TGGCTCCGTG ACCGCAACTT AAGACCCAAA 
TTCTCACAGA TCGTTAGTTC ACTTGACAAG CTAATACGTA ATGCAGCAAG TTTAAAGGTG 
ACCAGCCCAG CACAGGCAGG AGTATCTCAG CAGCTTCTAG ATCGCACTGT TCCTGATTAC 
ACTACTTTTC CCACTGTGAG CGATTGGTTG GAAGCCATTA AGATGGGGCA GTATCAAGAG 
AATTTTCTAA GCGCTGGCTT CACTTCTTTC CACTTGGTAG CACAAATGAC AGCTGAGGAC 
TTGCTCCGTA TCGGGGTGAC GCTGGCTGGT CACCAGAAAA AACTTCTCAA TAGTGTTCAG 
GACATGAGAC TGCAAATGAG TCAGACACTT CCTGTCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ephb3 ( XM_002935148.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935194.1 Xenopus tropicalis EPH receptor B3 (ephb3), mRNA.

Price & Availability↑

CloneID	OXa58160
Clone ID Related Accession (Same CDS sequence)	XM_002935148.5
Accession Version	XM_002935148.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephrin type-B receptor 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935148.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG CTGTGTTCGT GATGCTGGCG CTGTCGGCAG TGCAAGGGCT GGAAGAGACT 
CTGATGGACA CAAAATGGAC AACATCAGAA TTAGCATGGG TTGCATACCC AGATTCAGGG 
TGGGAGGAAG TAAGTGGATA CGATGAAGCC TCAAACCCAA TCCGGACGTA TCAGGTTTGT 
AATGTACGGG ATGGTAATCA GAATAATTGG CTACGTACGC AGTTTATTCC ACGGCAAGAT 
GTTCAGCGCG TGTATGTGGA GTTGAAGTTT ACTGTCAGAG ACTGTAACAG CCTGCCGAAT 
ATCCCAGGAT CTTGCAAAGA GACCTTTAAT TTCTTCTACT ATGAATCGGA CTCTGACTCT 
GCTTCTGCTG ACAGCCCATT CTGGATGGAA AACCCATACA TGAAGGTGGA TACCATAGCT 
CCAGATGAGA GCTTTTCACG ACGGGATTCT GGGCGTGTCA ACACAAAGAT ACGTAGCTTT 
GGACCTTTAT CCCGTACAGG TTTCTACTTG GCTTTTCAGG ACCTGGGTGC CTGTGTATCC 
CTAATTTCAG TTCGTGTCTT CTTTAAAAAG TGTCCTCGAA CCATTGCTGG ATTTGCCACT 
TTTCCCGAGA CAGTTACAGG GGCTGAAGCA ACATCACTTG TTATTGCACC AGGTACATGT 
GTGCCGAATG CCCTAGAAGT TTCTGTGCCT CTCAAGCTGT ACTGCAATGG AGATGGAGAA 
TGGATGGTGC CTGTGGGTGC CTGTACATGC GCATCTGGGT TTGAAGCAGC TGGGAAGGAC 
ACTCAATGCC AAGCATGCAA ACGTGGAACA TACAAGTCAA AGCAGGGAGA GGGAAGCTGT 
GTGCCTTGCC CCGTTAACAG CAGAGCCATT TCTTCAGCAG CCACTATCTG TTCCTGTCAG 
AATGGATACT ACCGTGCTGA CGGAGAGAGT GCTGAGACTG CGTGTACCTC TGTGCCCTCG 
GCACCACGAC ATGTTATCTC CAATGTAAAT GAAACCTCTG TGGTCCTTGA ATGGGCAGAG 
CCTGGTCATT TAGGCGGTCG GGATGACATT CTATACAATG TGATCTGTAA GAAATGCTTG 
GAGCGACTGT GTTCTCGCTG TGATGATAAT GTTCAGTTCT GGCCACGGCA GCTTGGTGTC 
ACCCAGAGGC GAGTCTCAGT CAGTCATCTC CAGGCCCACA CCAAGTATAG CTTTGAAATC 
CAGGCGGTCA ACGGAGTGTC TGGCAAAAGT CCACACATCC CCAACTACTA TACTGTGAAT 
ATTACAACTA ACCAGGCAGC CCCTTCAGCT GTGCCAATGG TGCAGTCTCA TGGAAGTTCG 
GCAAACTCTC TAACTCTGTC TTGGGCACCT CCAGAGAGCC CCAATGGCAT CATATTAGAC 
TATGAAATAA AATACTATCC CAAAGGCCAA AGCGGTGCAG GTAACACAGT GACCAGCCAG 
CGCACAACTG TGCGAATGGA AGCAATGACC CCAGATACAG TGTATGTTGT TCAGGTTCGA 
GCCCGTACTG TGGCTGGATA TGGAGCCTAT AGTGAACCTA GAGAGTTCCA GACAATCTCT 
GAAGATGGTG ACCGGAGCAG TCTGCAGGAA CAAGTCCCGA TGGTTGTTGG CTCTGTAACA 
GCCGGACTCA TTTTTATCAT TGCAGTGGTC ATTATAGTAA TAGTTTGTTT CAGCCGCAAG 
CAAAGGAATG ATGCTGAATC TGAATACACT GAAAAGCTGC AGCAGTACAT GGTGCCTGGC 
ATGAAGCTTT ACATTGATCC ATTTACGTAT GAGGATCCTA ATGAAGCTGT GCGAGACTTT 
GCTAAAGAAA TTGACATTTC CTGTGTGAAG ATTGAAGAAG TGATTGGTGC AGGTGAATTT 
GGGGAAGTGT GCCGGGGGAG ACTAAAGCAA GCAGGTCGCA GAGAACAGCT TGTAGCTATT 
AAGACATTGA AGGCAGGTTA CACAGAACAA CAAAGAAGAG ACTTTCTCGG TGAGGCCAGC 
ATCATGGGAC AGTTTGATCA TCCAAACATC ATTCGACTGG AAGGAGTTGT GACAAGGAGC 
CGGCCTGTCA TGATCTTGAC TGAATTTATG GAGAATGGAG CCCTTGACTC ATTTCTAAGG 
ATGAACGATG GACAGTTCAC AGTTATCCAG CTGGTTGGGA TACTCCGTGG CATTGCATCT 
GGAATGAAAT ATTTGTCCGA GATGAACTAT GTTCATCGGG ATCTTGCTGC AAGGAACATC 
CTGGTGAACA GTAACCTTGT CTGCAAAGTG TCTGATTTTG GTCTGTCTAG ATTTTTGGAA 
AACAGTCGCT CTGATCCAAC GTACACAAGT TCATTGGGAG GAAAGATTCC AATTCGTTGG 
ACTGCTCCAG AGGCTATCTC ATACAGAAAG TTCACTTCTG CAAGCGATGT CTGGAGTTAT 
GGCATTGTCA TGTGGGAGGT TATGTCTTAC GGAGAGCGGC CATATTGGGA CATGTCAAAT 
CAAGATGTAA TTAATGCAAT TGAGCAAGAC TATCGGCTGC CACCTCCTAT GGATTGTCCT 
GCTGCTCTAC ACCAGCTCAT GCTGGACTGC TGGCTCCGTG ACCGCAACTT AAGACCCAAA 
TTCTCACAGA TCGTTAGTTC ACTTGACAAG CTAATACGTA ATGCAGCAAG TTTAAAGGTG 
ACCAGCCCAG CACAGGCAGG AGTATCTCAG CAGCTTCTAG ATCGCACTGT TCCTGATTAC 
ACTACTTTTC CCACTGTGAG CGATTGGTTG GAAGCCATTA AGATGGGGCA GTATCAAGAG 
AATTTTCTAA GCGCTGGCTT CACTTCTTTC CACTTGGTAG CACAAATGAC AGCTGAGGAC 
TTGCTCCGTA TCGGGGTGAC GCTGGCTGGT CACCAGAAAA AACTTCTCAA TAGTGTTCAG 
GACATGAGAC TGCAAATGAG TCAGACACTT CCTGTCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935194.1
CDS	278..3202
Translation	MVPAVFVMLALSAVQGLEETLMDTKWTTSELAWVAYPDSGWEEVSGYDEASNPIRTYQVCNVRDGNQNNWLRTQFIPRQDVQRVYVELKFTVRDCNSLPNIPGSCKETFNFFYYESDSDSASADSPFWMENPYMKVDTIAPDESFSRRDSGRVNTKIRSFGPLSRTGFYLAFQDLGACVSLISVRVFFKKCPRTIAGFATFPETVTGAEATSLVIAPGTCVPNALEVSVPLKLYCNGDGEWMVPVGACTCASGFEAAGKDTQCQACKRGTYKSKQGEGSCVPCPVNSRAISSAATICSCQNGYYRADGESAETACTSVPSAPRHVISNVNETSVVLEWAEPGHLGGRDDILYNVICKKCLERLCSRCDDNVQFWPRQLGVTQRRVSVSHLQAHTKYSFEIQAVNGVSGKSPHIPNYYTVNITTNQAAPSAVPMVQSHGSSANSLTLSWAPPESPNGIILDYEIKYYPKGQSGAGNTVTSQRTTVRMEAMTPDTVYVVQVRARTVAGYGAYSEPREFQTISEDGDRSSLQEQVPMVVGSVTAGLIFIIAVVIIVIVCFSRKQRNDAESEYTEKLQQYMVPGMKLYIDPFTYEDPNEAVRDFAKEIDISCVKIEEVIGAGEFGEVCRGRLKQAGRREQLVAIKTLKAGYTEQQRRDFLGEASIMGQFDHPNIIRLEGVVTRSRPVMILTEFMENGALDSFLRMNDGQFTVIQLVGILRGIASGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLENSRSDPTYTSSLGGKIPIRWTAPEAISYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWLRDRNLRPKFSQIVSSLDKLIRNAASLKVTSPAQAGVSQQLLDRTVPDYTTFPTVSDWLEAIKMGQYQENFLSAGFTSFHLVAQMTAEDLLRIGVTLAGHQKKLLNSVQDMRLQMSQTLPVQV

Target ORF information:

RefSeq Version	XM_002935148.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EPH receptor B3 (ephb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935148.5

ORF Insert Sequence:

ATGGTCCCGG CTGTGTTCGT GATGCTGGCG CTGTCGGCAG TGCAAGGGCT GGAAGAGACT 
CTGATGGACA CAAAATGGAC AACATCAGAA TTAGCATGGG TTGCATACCC AGATTCAGGG 
TGGGAGGAAG TAAGTGGATA CGATGAAGCC TCAAACCCAA TCCGGACGTA TCAGGTTTGT 
AATGTACGGG ATGGTAATCA GAATAATTGG CTACGTACGC AGTTTATTCC ACGGCAAGAT 
GTTCAGCGCG TGTATGTGGA GTTGAAGTTT ACTGTCAGAG ACTGTAACAG CCTGCCGAAT 
ATCCCAGGAT CTTGCAAAGA GACCTTTAAT TTCTTCTACT ATGAATCGGA CTCTGACTCT 
GCTTCTGCTG ACAGCCCATT CTGGATGGAA AACCCATACA TGAAGGTGGA TACCATAGCT 
CCAGATGAGA GCTTTTCACG ACGGGATTCT GGGCGTGTCA ACACAAAGAT ACGTAGCTTT 
GGACCTTTAT CCCGTACAGG TTTCTACTTG GCTTTTCAGG ACCTGGGTGC CTGTGTATCC 
CTAATTTCAG TTCGTGTCTT CTTTAAAAAG TGTCCTCGAA CCATTGCTGG ATTTGCCACT 
TTTCCCGAGA CAGTTACAGG GGCTGAAGCA ACATCACTTG TTATTGCACC AGGTACATGT 
GTGCCGAATG CCCTAGAAGT TTCTGTGCCT CTCAAGCTGT ACTGCAATGG AGATGGAGAA 
TGGATGGTGC CTGTGGGTGC CTGTACATGC GCATCTGGGT TTGAAGCAGC TGGGAAGGAC 
ACTCAATGCC AAGCATGCAA ACGTGGAACA TACAAGTCAA AGCAGGGAGA GGGAAGCTGT 
GTGCCTTGCC CCGTTAACAG CAGAGCCATT TCTTCAGCAG CCACTATCTG TTCCTGTCAG 
AATGGATACT ACCGTGCTGA CGGAGAGAGT GCTGAGACTG CGTGTACCTC TGTGCCCTCG 
GCACCACGAC ATGTTATCTC CAATGTAAAT GAAACCTCTG TGGTCCTTGA ATGGGCAGAG 
CCTGGTCATT TAGGCGGTCG GGATGACATT CTATACAATG TGATCTGTAA GAAATGCTTG 
GAGCGACTGT GTTCTCGCTG TGATGATAAT GTTCAGTTCT GGCCACGGCA GCTTGGTGTC 
ACCCAGAGGC GAGTCTCAGT CAGTCATCTC CAGGCCCACA CCAAGTATAG CTTTGAAATC 
CAGGCGGTCA ACGGAGTGTC TGGCAAAAGT CCACACATCC CCAACTACTA TACTGTGAAT 
ATTACAACTA ACCAGGCAGC CCCTTCAGCT GTGCCAATGG TGCAGTCTCA TGGAAGTTCG 
GCAAACTCTC TAACTCTGTC TTGGGCACCT CCAGAGAGCC CCAATGGCAT CATATTAGAC 
TATGAAATAA AATACTATCC CAAAGGCCAA AGCGGTGCAG GTAACACAGT GACCAGCCAG 
CGCACAACTG TGCGAATGGA AGCAATGACC CCAGATACAG TGTATGTTGT TCAGGTTCGA 
GCCCGTACTG TGGCTGGATA TGGAGCCTAT AGTGAACCTA GAGAGTTCCA GACAATCTCT 
GAAGATGGTG ACCGGAGCAG TCTGCAGGAA CAAGTCCCGA TGGTTGTTGG CTCTGTAACA 
GCCGGACTCA TTTTTATCAT TGCAGTGGTC ATTATAGTAA TAGTTTGTTT CAGCCGCAAG 
CAAAGGAATG ATGCTGAATC TGAATACACT GAAAAGCTGC AGCAGTACAT GGTGCCTGGC 
ATGAAGCTTT ACATTGATCC ATTTACGTAT GAGGATCCTA ATGAAGCTGT GCGAGACTTT 
GCTAAAGAAA TTGACATTTC CTGTGTGAAG ATTGAAGAAG TGATTGGTGC AGGTGAATTT 
GGGGAAGTGT GCCGGGGGAG ACTAAAGCAA GCAGGTCGCA GAGAACAGCT TGTAGCTATT 
AAGACATTGA AGGCAGGTTA CACAGAACAA CAAAGAAGAG ACTTTCTCGG TGAGGCCAGC 
ATCATGGGAC AGTTTGATCA TCCAAACATC ATTCGACTGG AAGGAGTTGT GACAAGGAGC 
CGGCCTGTCA TGATCTTGAC TGAATTTATG GAGAATGGAG CCCTTGACTC ATTTCTAAGG 
ATGAACGATG GACAGTTCAC AGTTATCCAG CTGGTTGGGA TACTCCGTGG CATTGCATCT 
GGAATGAAAT ATTTGTCCGA GATGAACTAT GTTCATCGGG ATCTTGCTGC AAGGAACATC 
CTGGTGAACA GTAACCTTGT CTGCAAAGTG TCTGATTTTG GTCTGTCTAG ATTTTTGGAA 
AACAGTCGCT CTGATCCAAC GTACACAAGT TCATTGGGAG GAAAGATTCC AATTCGTTGG 
ACTGCTCCAG AGGCTATCTC ATACAGAAAG TTCACTTCTG CAAGCGATGT CTGGAGTTAT 
GGCATTGTCA TGTGGGAGGT TATGTCTTAC GGAGAGCGGC CATATTGGGA CATGTCAAAT 
CAAGATGTAA TTAATGCAAT TGAGCAAGAC TATCGGCTGC CACCTCCTAT GGATTGTCCT 
GCTGCTCTAC ACCAGCTCAT GCTGGACTGC TGGCTCCGTG ACCGCAACTT AAGACCCAAA 
TTCTCACAGA TCGTTAGTTC ACTTGACAAG CTAATACGTA ATGCAGCAAG TTTAAAGGTG 
ACCAGCCCAG CACAGGCAGG AGTATCTCAG CAGCTTCTAG ATCGCACTGT TCCTGATTAC 
ACTACTTTTC CCACTGTGAG CGATTGGTTG GAAGCCATTA AGATGGGGCA GTATCAAGAG 
AATTTTCTAA GCGCTGGCTT CACTTCTTTC CACTTGGTAG CACAAATGAC AGCTGAGGAC 
TTGCTCCGTA TCGGGGTGAC GCTGGCTGGT CACCAGAAAA AACTTCTCAA TAGTGTTCAG 
GACATGAGAC TGCAAATGAG TCAGACACTT CCTGTCCAGG TGTGA