Ephb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ephb3
Entrez Gene ID	13845
Full Name	Eph receptor B3
Synonyms	AW456895,Cek10,Etk2,HEK2,MDK5,Sek4,Tyro6
General protein information	Preferred Names Eph receptor B3 Names ephrin type-B receptor 3 developmental kinase 5 mDK-5 tyrosine-protein kinase receptor SEK-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006521788.2	XP_006521851.1	ephrin type-B receptor 3 isoform X1
XM_006521788.1	XP_006521851.1	ephrin type-B receptor 3 isoform X1
NM_010143.1	NP_034273.1	ephrin type-B receptor 3 precursor

Pathways from BioSystems

Homologies

	EPHB3	XP_001097522.2
Rattus norvegicus (Norway rat)	Ephb3	NP_001099338.1
Gallus gallus (chicken)	EPHB3	XP_422762.4
Homo sapiens (human)	EPHB3	NP_004434.2
Pan troglodytes (chimpanzee)	EPHB3	XP_003310198.1
Bos taurus (cattle)	EPHB3	NP_001179725.1
Danio rerio (zebrafish)	LOC555957	XP_683712.6
Canis lupus familiaris (dog)	EPHB3	XP_545232.3
Mus musculus (house mouse)	Ephb3	NP_034273.1
Xenopus tropicalis (tropical clawed frog)	LOC100489199	XP_002935194.1

Gene Ontology
Bibliography

Related articles in PubMed

EphB receptors, mainly EphB3, contribute to the proper development of cortical thymic epithelial cells.
Montero-Herrad?n S, Garc?a-Ceca J, Zapata AG
Organogenesis13(4)192-211(2017 Oct)

Eph/Ephrin Signaling Controls Progenitor Identities In The Ventral Spinal Cord.
Laussu J, Audouard C, Kischel A, Assis-Nascimento P, Escalas N, Liebl DJ, Soula C, Davy A
Neural development12(1)10(2017 Jun)

Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.
O'Neill AK, Kindberg AA, Niethamer TK, Larson AR, Ho HH, Greenberg ME, Bush JO
The Journal of cell biology215(2)217-229(2016 Oct)

Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons.
Sandberg M, Flandin P, Silberberg S, Su-Feher L, Price JD, Hu JS, Kim C, Visel A, Nord AS, Rubenstein JLR
Neuron91(6)1260-1275(2016 Sep)

Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain.
Becker N, Seitanidou T, Murphy P, Matt?i MG, Topilko P, Nieto MA, Wilkinson DG, Charnay P, Gilardi-Hebenstreit P
Mechanisms of development47(1)3-17(1994 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mule also regulates protein levels of the receptor tyrosine kinase EphB3 by targeting it for proteasomal and lysosomal degradation.
Title: Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation.

These results suggest a major function for forward signaling through EphB2 and, to a lesser extent, EphB3, in either colonizing progenitor cells or thymic stromal cells.
Title: EphB2 and EphB3 play an important role in the lymphoid seeding of murine adult thymus.

A novel dependence receptor role of EphB3 in oligodendrocyte cell death after spinal cord injury.
Title: EphB3 receptors function as dependence receptors to mediate oligodendrocyte cell death following contusive spinal cord injury.

Conclude that EphB3 mediates cell death in the adult cortex through a novel dependence receptor-mediated cell death mechanism in the injured adult cortex and is attenuated following ephrinB3 stimulation.
Title: EphrinB3 blocks EphB3 dependence receptor functions to prevent cell death following traumatic brain injury.

EphB2 and EphB3 are involved in the control of thymic epithelial cells (TEC) survival and that the absence of these molecules causes increased apoptotic TEC.
Title: Eph/ephrinB signalling is involved in the survival of thymic epithelial cells.

The following Ephb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ephb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05622	NM_010143.1 Latest version!	Mus musculus Eph receptor B3 (Ephb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$580.30 $829.00
OMu51870	XM_006521788.2 Latest version!	Mus musculus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu51870	XM_006521788.1	Mus musculus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ephb3 ( NM_010143.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034273.1 Mus musculus Eph receptor B3 (Ephb3), mRNA.

Price & Availability↑

CloneID	OMu05622
Clone ID Related Accession (Same CDS sequence)	NM_010143.1
Accession Version	NM_010143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053085.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053085.1, AK144414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTTC CCGCCGGCTG CTGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCTGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT AAGCGGCTAT 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ATGTGCGTGA GTCGAGCCAG 
AACAACTGGC TGCGGACGGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTCA CCGTAAGAGA CTGTAACAGC ATTCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTCTACTA CGAGGCTGAT AGCGATGTGG CCTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTCGATGCCG GGCGTGTCAA CACCAAGGTG CGCAGCTTCG GGCCGCTTTC CAAGGCTGGC 
TTCTACTTGG CCTTCCAGGA CCAAGGTGCC TGCATGTCAC TCATCTCTGT GCGTGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCC GGCACCTGCA TCGCTAACGC TGTGGAGGTC 
TCTGTACCAC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTCGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGT CCCAGTGCCG TGCCTGTCCT 
CCTGGGAGTT ACAAAGCAAA GCAAGGAGAA GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGCCATA ATAACTTCTA CCGTGCAGAC 
TCAGACTCGG CCGACAGCGC CTGCACCACG GTGCCATCTC CACCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT AATCCTCGAA TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGCAAG AAGTGCCGTG GGAGCTCTGG GGCTGGGGGT 
CCGGCAACCT GTTCACGCTG CGATGACAAC GTAGAGTTTG TACCTCGGCA GCTGGGCCTG 
ACAGAGCGCC GGGTCCACAT TAGCCACCTG TTGGCCCACA CACGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGTGTCTC AGGCAAAAGC CCTTTGCCAC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCTGC TCCATCAGAA GTGCCTACAC TCCACCTGCA CAGCAGCTCG 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCAGAGCGGC CTAATGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTAAC CAGCCAGAAG 
AACTCCGTAC AACTGGACGG GCTGCAGCCT GATGCCCGCT ACGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CGGGTTACGG ACAGTATAGC CACCCGGCTG AGTTTGAGAC CACAAGTGAA 
AGAGGTTCAG GTGCCCAGCA GCTTCAAGAA CAGCTTCCCC TGATTGTGGG ATCCACCGTA 
GCCGGGTTTG TCTTCATGGT GGTCGTCGTG GTCATCGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCATGGCC CCGATGCAGA ATACACAGAG AAGCTGCAGC AATACATTGC TCCTGGGATG 
AAAGTTTACA TTGACCCCTT TACCTATGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG TGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGACT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCTATCAAG 
ACGCTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTAAGTGA GGCTTCCATT 
ATGGGTCAGT TTGACCATCC CAATATAATC CGTCTAGAGG GTGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT TCTACGGCTC 
AATGACGGAC AGTTCACGGT CATCCAGCTT GTGGGCATGT TACGAGGCAT TGCTGCTGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TTGCTGCCCG AAACATCCTT 
GTCAACAGCA ACTTGGTCTG CAAAGTCTCT GACTTTGGCC TCTCCCGCTT CCTGGAGGAT 
GACCCATCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGCTGGACC 
GCCCCGGAGG CCATAGCCTA TCGGAAGTTT ACGTCTGCCA GCGATGTCTG GAGCTATGGG 
ATTGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCCT ACTGGGATAT GAGCAACCAG 
GATGTCATTA ATGCTGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACC 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCTCAAATCG TCAACACACT AGACAAACTT ATCCGCAATG CTGCCAGCCT CAAGGTCACC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTGGACC GCACAGTCCC AGATTATACG 
ACCTTCACGA CAGTGGGTGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTTGTCGGTG CTGGGTTTGC ATCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATTG GGGTCACTTT GGCTGGGCAC CAGAAGAAGA TCCTCTGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACCCTGCCT GTGCAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034273.1
CDS	376..3357
Translation	MAGARPPPGLLPLLAPLLLPLLLPAGCWALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSHPAEFETTSERGSGAQQLQEQLPLIVGSTVAGFVFMVVVVVIALVCLRKQRHGPDAEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVTASAPSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILCSIQDMRLQMNQTLPVQV

Target ORF information:

RefSeq Version	NM_010143.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B3 (Ephb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010143.1

ORF Insert Sequence:

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTTC CCGCCGGCTG CTGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCTGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT AAGCGGCTAT 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ATGTGCGTGA GTCGAGCCAG 
AACAACTGGC TGCGGACGGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTCA CCGTAAGAGA CTGTAACAGC ATTCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTCTACTA CGAGGCTGAT AGCGATGTGG CCTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTCGATGCCG GGCGTGTCAA CACCAAGGTG CGCAGCTTCG GGCCGCTTTC CAAGGCTGGC 
TTCTACTTGG CCTTCCAGGA CCAAGGTGCC TGCATGTCAC TCATCTCTGT GCGTGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCC GGCACCTGCA TCGCTAACGC TGTGGAGGTC 
TCTGTACCAC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTCGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGT CCCAGTGCCG TGCCTGTCCT 
CCTGGGAGTT ACAAAGCAAA GCAAGGAGAA GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGCCATA ATAACTTCTA CCGTGCAGAC 
TCAGACTCGG CCGACAGCGC CTGCACCACG GTGCCATCTC CACCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT AATCCTCGAA TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGCAAG AAGTGCCGTG GGAGCTCTGG GGCTGGGGGT 
CCGGCAACCT GTTCACGCTG CGATGACAAC GTAGAGTTTG TACCTCGGCA GCTGGGCCTG 
ACAGAGCGCC GGGTCCACAT TAGCCACCTG TTGGCCCACA CACGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGTGTCTC AGGCAAAAGC CCTTTGCCAC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCTGC TCCATCAGAA GTGCCTACAC TCCACCTGCA CAGCAGCTCG 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCAGAGCGGC CTAATGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTAAC CAGCCAGAAG 
AACTCCGTAC AACTGGACGG GCTGCAGCCT GATGCCCGCT ACGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CGGGTTACGG ACAGTATAGC CACCCGGCTG AGTTTGAGAC CACAAGTGAA 
AGAGGTTCAG GTGCCCAGCA GCTTCAAGAA CAGCTTCCCC TGATTGTGGG ATCCACCGTA 
GCCGGGTTTG TCTTCATGGT GGTCGTCGTG GTCATCGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCATGGCC CCGATGCAGA ATACACAGAG AAGCTGCAGC AATACATTGC TCCTGGGATG 
AAAGTTTACA TTGACCCCTT TACCTATGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG TGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGACT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCTATCAAG 
ACGCTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTAAGTGA GGCTTCCATT 
ATGGGTCAGT TTGACCATCC CAATATAATC CGTCTAGAGG GTGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT TCTACGGCTC 
AATGACGGAC AGTTCACGGT CATCCAGCTT GTGGGCATGT TACGAGGCAT TGCTGCTGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TTGCTGCCCG AAACATCCTT 
GTCAACAGCA ACTTGGTCTG CAAAGTCTCT GACTTTGGCC TCTCCCGCTT CCTGGAGGAT 
GACCCATCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGCTGGACC 
GCCCCGGAGG CCATAGCCTA TCGGAAGTTT ACGTCTGCCA GCGATGTCTG GAGCTATGGG 
ATTGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCCT ACTGGGATAT GAGCAACCAG 
GATGTCATTA ATGCTGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACC 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCTCAAATCG TCAACACACT AGACAAACTT ATCCGCAATG CTGCCAGCCT CAAGGTCACC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTGGACC GCACAGTCCC AGATTATACG 
ACCTTCACGA CAGTGGGTGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTTGTCGGTG CTGGGTTTGC ATCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATTG GGGTCACTTT GGCTGGGCAC CAGAAGAAGA TCCTCTGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACCCTGCCT GTGCAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ephb3 ( XM_006521788.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521851.1 Mus musculus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51870
Clone ID Related Accession (Same CDS sequence)	XM_006521788.2 , XM_006521788.1
Accession Version	XM_006521788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTTC CCGCCGGCTG CTGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCTGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT AAGCGGCTAT 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ATGTGCGTGA GTCGAGCCAG 
AACAACTGGC TGCGGACGGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTCA CCGTAAGAGA CTGTAACAGC ATTCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTCTACTA CGAGGCTGAT AGCGATGTGG CCTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTCGATGCCG GGCGTGTCAA CACCAAGGTG CGCAGCTTCG GGCCGCTTTC CAAGGCTGGC 
TTCTACTTGG CCTTCCAGGA CCAAGGTGCC TGCATGTCAC TCATCTCTGT GCGTGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCC GGCACCTGCA TCGCTAACGC TGTGGAGGTC 
TCTGTACCAC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTCGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGT CCCAGTGCCG TGCCTGTCCT 
CCTGGGAGTT ACAAAGCAAA GCAAGGAGAA GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGCCATA ATAACTTCTA CCGTGCAGAC 
TCAGACTCGG CCGACAGCGC CTGCACCACG GTGCCATCTC CACCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT AATCCTCGAA TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGCAAG AAGTGCCGTG GGAGCTCTGG GGCTGGGGGT 
CCGGCAACCT GTTCACGCTG CGATGACAAC GTAGAGTTTG TACCTCGGCA GCTGGGCCTG 
ACAGAGCGCC GGGTCCACAT TAGCCACCTG TTGGCCCACA CACGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGTGTCTC AGGCAAAAGC CCTTTGCCAC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCTGC TCCATCAGAA GTGCCTACAC TCCACCTGCA CAGCAGCTCG 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCAGAGCGGC CTAATGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTAAC CAGCCAGAAG 
AACTCCGTAC AACTGGACGG GCTGCAGCCT GATGCCCGCT ACGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CGGGTTACGG ACAGTATAGC CACCCGGCTG AGTTTGAGAC CACAAGTGAA 
AGAGGTTCAG GTGCCCAGCA GCTTCAAGAA CAGCTTCCCC TGATTGTGGG ATCCACCGTA 
GCCGGGTTTG TCTTCATGGT GGTCGTCGTG GTCATCGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCATGGCC CCGATGCAGA ATACACAGAG AAGCTGCAGC AATACATTGC TCCTGGGATG 
AAAGTTTACA TTGACCCCTT TACCTATGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG TGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGACT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCTATCAAG 
ACGCTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTAAGTGA GGCTTCCATT 
ATGGGTCAGT TTGACCATCC CAATATAATC CGTCTAGAGG GTGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT TCTACGGCTC 
AATGACGGAC AGTTCACGGT CATCCAGCTT GTGGGCATGT TACGAGGCAT TGCTGCTGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TTGCTGCCCG AAACATCCTT 
GTCAACAGCA ACTTGGTCTG CAAAGTCTCT GACTTTGGCC TCTCCCGCTT CCTGGAGGAT 
GACCCATCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGCTGGACC 
GCCCCGGAGG CCATAGCCTA TCGGAAGTTT ACGTCTGCCA GCGATGTCTG GAGCTATGGG 
ATTGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCCT ACTGGGATAT GAGCAACCAG 
GATGTCATTA ATGCTGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACC 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCTCAAATCG TCAACACACT AGACAAACTT ATCCGCAATG CTGCCAGCCT CAAGGTCACC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTGGACC GCACAGTCCC AGATTATACG 
ACCTTCACGA CAGTGGGTGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTTGTCGGTG CTGGGTTTGC ATCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATTG GGGTCACTTT GGCTGGGCAC CAGAAGAAGA TCCTCTGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACCCTGCCT GTGCAGGAAG TGTGCCCCAA ACCTCTTCAT 
ATTGAAGATG GATTAGAAGA GGGGGTGATG TCCCCTCCCC AGATGCCTTG GGGCCCAGGC 
CTGCCTGCTC TCCAGTGGGG AGTCTTCACA ACTCAGATTG GGCTGCGCTT CAGTAATGGA 
CGTCCTGGTA GGGTCAGGTG GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521851.1
CDS	551..3697
Translation	MAGARPPPGLLPLLAPLLLPLLLPAGCWALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSHPAEFETTSERGSGAQQLQEQLPLIVGSTVAGFVFMVVVVVIALVCLRKQRHGPDAEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVTASAPSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILCSIQDMRLQMNQTLPVQEVCPKPLHIEDGLEEGVMSPPQMPWGPGLPALQWGVFTTQIGLRFSNGRPGRVRWG

Target ORF information:

RefSeq Version	XM_006521788.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521788.2

ORF Insert Sequence:

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTTC CCGCCGGCTG CTGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCTGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT AAGCGGCTAT 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ATGTGCGTGA GTCGAGCCAG 
AACAACTGGC TGCGGACGGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTCA CCGTAAGAGA CTGTAACAGC ATTCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTCTACTA CGAGGCTGAT AGCGATGTGG CCTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTCGATGCCG GGCGTGTCAA CACCAAGGTG CGCAGCTTCG GGCCGCTTTC CAAGGCTGGC 
TTCTACTTGG CCTTCCAGGA CCAAGGTGCC TGCATGTCAC TCATCTCTGT GCGTGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCC GGCACCTGCA TCGCTAACGC TGTGGAGGTC 
TCTGTACCAC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTCGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGT CCCAGTGCCG TGCCTGTCCT 
CCTGGGAGTT ACAAAGCAAA GCAAGGAGAA GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGCCATA ATAACTTCTA CCGTGCAGAC 
TCAGACTCGG CCGACAGCGC CTGCACCACG GTGCCATCTC CACCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT AATCCTCGAA TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGCAAG AAGTGCCGTG GGAGCTCTGG GGCTGGGGGT 
CCGGCAACCT GTTCACGCTG CGATGACAAC GTAGAGTTTG TACCTCGGCA GCTGGGCCTG 
ACAGAGCGCC GGGTCCACAT TAGCCACCTG TTGGCCCACA CACGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGTGTCTC AGGCAAAAGC CCTTTGCCAC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCTGC TCCATCAGAA GTGCCTACAC TCCACCTGCA CAGCAGCTCG 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCAGAGCGGC CTAATGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTAAC CAGCCAGAAG 
AACTCCGTAC AACTGGACGG GCTGCAGCCT GATGCCCGCT ACGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CGGGTTACGG ACAGTATAGC CACCCGGCTG AGTTTGAGAC CACAAGTGAA 
AGAGGTTCAG GTGCCCAGCA GCTTCAAGAA CAGCTTCCCC TGATTGTGGG ATCCACCGTA 
GCCGGGTTTG TCTTCATGGT GGTCGTCGTG GTCATCGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCATGGCC CCGATGCAGA ATACACAGAG AAGCTGCAGC AATACATTGC TCCTGGGATG 
AAAGTTTACA TTGACCCCTT TACCTATGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG TGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGACT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCTATCAAG 
ACGCTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTAAGTGA GGCTTCCATT 
ATGGGTCAGT TTGACCATCC CAATATAATC CGTCTAGAGG GTGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT TCTACGGCTC 
AATGACGGAC AGTTCACGGT CATCCAGCTT GTGGGCATGT TACGAGGCAT TGCTGCTGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TTGCTGCCCG AAACATCCTT 
GTCAACAGCA ACTTGGTCTG CAAAGTCTCT GACTTTGGCC TCTCCCGCTT CCTGGAGGAT 
GACCCATCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGCTGGACC 
GCCCCGGAGG CCATAGCCTA TCGGAAGTTT ACGTCTGCCA GCGATGTCTG GAGCTATGGG 
ATTGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCCT ACTGGGATAT GAGCAACCAG 
GATGTCATTA ATGCTGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACC 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCTCAAATCG TCAACACACT AGACAAACTT ATCCGCAATG CTGCCAGCCT CAAGGTCACC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTGGACC GCACAGTCCC AGATTATACG 
ACCTTCACGA CAGTGGGTGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTTGTCGGTG CTGGGTTTGC ATCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATTG GGGTCACTTT GGCTGGGCAC CAGAAGAAGA TCCTCTGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACCCTGCCT GTGCAGGAAG TGTGCCCCAA ACCTCTTCAT 
ATTGAAGATG GATTAGAAGA GGGGGTGATG TCCCCTCCCC AGATGCCTTG GGGCCCAGGC 
CTGCCTGCTC TCCAGTGGGG AGTCTTCACA ACTCAGATTG GGCTGCGCTT CAGTAATGGA 
CGTCCTGGTA GGGTCAGGTG GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ephb3 ( XM_006521788.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521851.1 Mus musculus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.

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CloneID	OMu51870
Clone ID Related Accession (Same CDS sequence)	XM_006521788.2 , XM_006521788.1
Accession Version	XM_006521788.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ephrin type-B receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTTC CCGCCGGCTG CTGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCTGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT AAGCGGCTAT 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ATGTGCGTGA GTCGAGCCAG 
AACAACTGGC TGCGGACGGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTCA CCGTAAGAGA CTGTAACAGC ATTCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTCTACTA CGAGGCTGAT AGCGATGTGG CCTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTCGATGCCG GGCGTGTCAA CACCAAGGTG CGCAGCTTCG GGCCGCTTTC CAAGGCTGGC 
TTCTACTTGG CCTTCCAGGA CCAAGGTGCC TGCATGTCAC TCATCTCTGT GCGTGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCC GGCACCTGCA TCGCTAACGC TGTGGAGGTC 
TCTGTACCAC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTCGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGT CCCAGTGCCG TGCCTGTCCT 
CCTGGGAGTT ACAAAGCAAA GCAAGGAGAA GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGCCATA ATAACTTCTA CCGTGCAGAC 
TCAGACTCGG CCGACAGCGC CTGCACCACG GTGCCATCTC CACCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT AATCCTCGAA TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGCAAG AAGTGCCGTG GGAGCTCTGG GGCTGGGGGT 
CCGGCAACCT GTTCACGCTG CGATGACAAC GTAGAGTTTG TACCTCGGCA GCTGGGCCTG 
ACAGAGCGCC GGGTCCACAT TAGCCACCTG TTGGCCCACA CACGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGTGTCTC AGGCAAAAGC CCTTTGCCAC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCTGC TCCATCAGAA GTGCCTACAC TCCACCTGCA CAGCAGCTCG 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCAGAGCGGC CTAATGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTAAC CAGCCAGAAG 
AACTCCGTAC AACTGGACGG GCTGCAGCCT GATGCCCGCT ACGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CGGGTTACGG ACAGTATAGC CACCCGGCTG AGTTTGAGAC CACAAGTGAA 
AGAGGTTCAG GTGCCCAGCA GCTTCAAGAA CAGCTTCCCC TGATTGTGGG ATCCACCGTA 
GCCGGGTTTG TCTTCATGGT GGTCGTCGTG GTCATCGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCATGGCC CCGATGCAGA ATACACAGAG AAGCTGCAGC AATACATTGC TCCTGGGATG 
AAAGTTTACA TTGACCCCTT TACCTATGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG TGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGACT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCTATCAAG 
ACGCTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTAAGTGA GGCTTCCATT 
ATGGGTCAGT TTGACCATCC CAATATAATC CGTCTAGAGG GTGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT TCTACGGCTC 
AATGACGGAC AGTTCACGGT CATCCAGCTT GTGGGCATGT TACGAGGCAT TGCTGCTGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TTGCTGCCCG AAACATCCTT 
GTCAACAGCA ACTTGGTCTG CAAAGTCTCT GACTTTGGCC TCTCCCGCTT CCTGGAGGAT 
GACCCATCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGCTGGACC 
GCCCCGGAGG CCATAGCCTA TCGGAAGTTT ACGTCTGCCA GCGATGTCTG GAGCTATGGG 
ATTGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCCT ACTGGGATAT GAGCAACCAG 
GATGTCATTA ATGCTGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACC 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCTCAAATCG TCAACACACT AGACAAACTT ATCCGCAATG CTGCCAGCCT CAAGGTCACC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTGGACC GCACAGTCCC AGATTATACG 
ACCTTCACGA CAGTGGGTGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTTGTCGGTG CTGGGTTTGC ATCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATTG GGGTCACTTT GGCTGGGCAC CAGAAGAAGA TCCTCTGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACCCTGCCT GTGCAGGAAG TGTGCCCCAA ACCTCTTCAT 
ATTGAAGATG GATTAGAAGA GGGGGTGATG TCCCCTCCCC AGATGCCTTG GGGCCCAGGC 
CTGCCTGCTC TCCAGTGGGG AGTCTTCACA ACTCAGATTG GGCTGCGCTT CAGTAATGGA 
CGTCCTGGTA GGGTCAGGTG GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521851.1
CDS	42..3188
Translation	MAGARPPPGLLPLLAPLLLPLLLPAGCWALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSHPAEFETTSERGSGAQQLQEQLPLIVGSTVAGFVFMVVVVVIALVCLRKQRHGPDAEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVTASAPSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILCSIQDMRLQMNQTLPVQEVCPKPLHIEDGLEEGVMSPPQMPWGPGLPALQWGVFTTQIGLRFSNGRPGRVRWG

Target ORF information:

RefSeq Version	XM_006521788.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eph receptor B3 (Ephb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521788.1

ORF Insert Sequence:

ATGGCCGGAG CCCGCCCGCC GCCGGGGCTT CTGCCGCTGC TCGCTCCGCT GCTGCTGCCG 
CTGCTGCTTC CCGCCGGCTG CTGGGCGCTG GAAGAGACTC TCATGGACAC GAAATGGGTG 
ACGTCTGAGC TGGCATGGAC ATCTCATCCA GAGAGTGGGT GGGAAGAAGT AAGCGGCTAT 
GATGAAGCCA TGAATCCTAT CCGCACGTAT CAGGTGTGTA ATGTGCGTGA GTCGAGCCAG 
AACAACTGGC TGCGGACGGG TTTCATCTGG CGGCGGGAAG TCCAGCGCGT CTACGTGGAG 
CTGAAGTTCA CCGTAAGAGA CTGTAACAGC ATTCCCAACA TCCCTGGCTC CTGCAAGGAA 
ACCTTCAACC TTTTCTACTA CGAGGCTGAT AGCGATGTGG CCTCAGCCTC CTCTCCCTTC 
TGGATGGAGA ACCCCTACGT GAAAGTGGAC ACCATTGCGC CAGATGAGAG CTTCTCGCGG 
CTCGATGCCG GGCGTGTCAA CACCAAGGTG CGCAGCTTCG GGCCGCTTTC CAAGGCTGGC 
TTCTACTTGG CCTTCCAGGA CCAAGGTGCC TGCATGTCAC TCATCTCTGT GCGTGCCTTC 
TACAAGAAGT GTGCATCCAC CACTGCAGGC TTCGCACTCT TCCCCGAGAC CCTCACGGGG 
GCTGAGCCCA CTTCGCTGGT CATTGCCCCC GGCACCTGCA TCGCTAACGC TGTGGAGGTC 
TCTGTACCAC TCAAGCTCTA CTGCAATGGC GACGGGGAGT GGATGGTGCC CGTCGGTGCC 
TGCACCTGCG CTACTGGCCA TGAGCCAGCC GCCAAGGAGT CCCAGTGCCG TGCCTGTCCT 
CCTGGGAGTT ACAAAGCAAA GCAAGGAGAA GGGCCCTGCC TCCCCTGTCC CCCCAATAGC 
CGCACCACCT CGCCGGCTGC CAGCATCTGC ACCTGCCATA ATAACTTCTA CCGTGCAGAC 
TCAGACTCGG CCGACAGCGC CTGCACCACG GTGCCATCTC CACCCCGGGG TGTGATCTCC 
AATGTGAATG AGACCTCGCT AATCCTCGAA TGGAGTGAGC CCCGGGACCT TGGCGGACGA 
GATGACCTCC TTTATAATGT TATCTGCAAG AAGTGCCGTG GGAGCTCTGG GGCTGGGGGT 
CCGGCAACCT GTTCACGCTG CGATGACAAC GTAGAGTTTG TACCTCGGCA GCTGGGCCTG 
ACAGAGCGCC GGGTCCACAT TAGCCACCTG TTGGCCCACA CACGCTACAC CTTTGAGGTG 
CAGGCTGTCA ACGGTGTCTC AGGCAAAAGC CCTTTGCCAC CCCGCTATGC AGCTGTGAAT 
ATCACCACCA ACCAGGCTGC TCCATCAGAA GTGCCTACAC TCCACCTGCA CAGCAGCTCG 
GGGAGCAGCC TGACCCTGTC CTGGGCACCC CCAGAGCGGC CTAATGGAGT CATCTTGGAC 
TATGAGATGA AGTACTTTGA GAAGAGTAAA GGCATCGCCT CCACTGTAAC CAGCCAGAAG 
AACTCCGTAC AACTGGACGG GCTGCAGCCT GATGCCCGCT ACGTAGTTCA GGTCCGGGCT 
CGCACAGTAG CGGGTTACGG ACAGTATAGC CACCCGGCTG AGTTTGAGAC CACAAGTGAA 
AGAGGTTCAG GTGCCCAGCA GCTTCAAGAA CAGCTTCCCC TGATTGTGGG ATCCACCGTA 
GCCGGGTTTG TCTTCATGGT GGTCGTCGTG GTCATCGCTC TTGTCTGCCT CAGGAAGCAG 
CGCCATGGCC CCGATGCAGA ATACACAGAG AAGCTGCAGC AATACATTGC TCCTGGGATG 
AAAGTTTACA TTGACCCCTT TACCTATGAG GATCCCAATG AGGCCGTCCG AGAGTTCGCC 
AAGGAGATCG ATGTGTCCTG TGTCAAGATC GAGGAGGTGA TTGGAGCTGG GGAGTTTGGG 
GAAGTGTGCC GGGGTCGACT GAAACTGCCC GGCCGCCGGG AGGTGTTCGT GGCTATCAAG 
ACGCTGAAGG TGGGATACAC GGAGAGGCAG CGGCGGGACT TCCTAAGTGA GGCTTCCATT 
ATGGGTCAGT TTGACCATCC CAATATAATC CGTCTAGAGG GTGTGGTCAC CAAAAGTCGT 
CCAGTCATGA TCCTCACTGA GTTCATGGAG AACTGTGCCC TGGACTCCTT TCTACGGCTC 
AATGACGGAC AGTTCACGGT CATCCAGCTT GTGGGCATGT TACGAGGCAT TGCTGCTGGC 
ATGAAGTACT TGTCTGAGAT GAACTACGTG CACCGTGACC TTGCTGCCCG AAACATCCTT 
GTCAACAGCA ACTTGGTCTG CAAAGTCTCT GACTTTGGCC TCTCCCGCTT CCTGGAGGAT 
GACCCATCAG ACCCCACCTA CACCAGCTCC CTGGGTGGGA AGATCCCTAT CCGCTGGACC 
GCCCCGGAGG CCATAGCCTA TCGGAAGTTT ACGTCTGCCA GCGATGTCTG GAGCTATGGG 
ATTGTCATGT GGGAGGTCAT GAGCTACGGA GAGCGACCCT ACTGGGATAT GAGCAACCAG 
GATGTCATTA ATGCTGTAGA GCAAGACTAT CGGTTACCAC CCCCCATGGA CTGCCCCACC 
GCGCTGCACC AGCTCATGCT GGACTGTTGG GTGCGGGACC GGAACCTCAG GCCCAAGTTC 
TCTCAAATCG TCAACACACT AGACAAACTT ATCCGCAATG CTGCCAGCCT CAAGGTCACC 
GCCAGTGCCC CATCTGGCAT GTCCCAGCCC CTCCTGGACC GCACAGTCCC AGATTATACG 
ACCTTCACGA CAGTGGGTGA CTGGCTAGAT GCCATCAAGA TGGGGAGGTA TAAAGAGAGC 
TTTGTCGGTG CTGGGTTTGC ATCCTTTGAC CTGGTGGCCC AGATGACTGC AGAAGATCTG 
CTAAGGATTG GGGTCACTTT GGCTGGGCAC CAGAAGAAGA TCCTCTGCAG TATCCAGGAC 
ATGCGGCTGC AGATGAACCA GACCCTGCCT GTGCAGGAAG TGTGCCCCAA ACCTCTTCAT 
ATTGAAGATG GATTAGAAGA GGGGGTGATG TCCCCTCCCC AGATGCCTTG GGGCCCAGGC 
CTGCCTGCTC TCCAGTGGGG AGTCTTCACA ACTCAGATTG GGCTGCGCTT CAGTAATGGA 
CGTCCTGGTA GGGTCAGGTG GGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EphB receptors, mainly EphB3, contribute to the proper development of cortical thymic epithelial cells.
Organogenesis13(4)192-211(2017 Oct)
Montero-Herrad?n S,Garc?a-Ceca J,Zapata AG

Eph/Ephrin Signaling Controls Progenitor Identities In The Ventral Spinal Cord.
Neural development12(1)10(2017 Jun)
Laussu J,Audouard C,Kischel A,Assis-Nascimento P,Escalas N,Liebl DJ,Soula C,Davy A

Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.
The Journal of cell biology215(2)217-229(2016 Oct)
O'Neill AK,Kindberg AA,Niethamer TK,Larson AR,Ho HH,Greenberg ME,Bush JO

Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons.
Neuron91(6)1260-1275(2016 Sep)
Sandberg M,Flandin P,Silberberg S,Su-Feher L,Price JD,Hu JS,Kim C,Visel A,Nord AS,Rubenstein JLR

Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain.
Mechanisms of development47(1)3-17(1994 Jul)
Becker N,Seitanidou T,Murphy P,Matt?i MG,Topilko P,Nieto MA,Wilkinson DG,Charnay P,Gilardi-Hebenstreit P

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Ephb3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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