kcnj1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kcnj1
Entrez Gene ID	100487609
Full Name	potassium voltage-gated channel subfamily J member 1
Synonyms	ROMK,kir1.1,romk1
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 1 Names ATP-sensitive inward rectifier potassium channel 1 potassium channel, inwardly rectifying subfamily J, member 1 potassium inwardly-rectifying channel, subfamily J, member 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004916171.4	XP_004916228.1	ATP-sensitive inward rectifier potassium channel 1
XM_004916171.3	XP_004916228.1	ATP-sensitive inward rectifier potassium channel 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KCNJ1	NP_001179136.1
Rattus norvegicus (Norway rat)	Kcnj1	NP_058719.1
	KCNJ1	XP_001113379.1
Danio rerio (zebrafish)	kcnj1b	NP_001092204.1
Xenopus tropicalis (tropical clawed frog)	kcnj1	XP_004916228.1
Pan troglodytes (chimpanzee)	KCNJ1	XP_522246.2
Mus musculus (house mouse)	Kcnj1	NP_001161826.1
Homo sapiens (human)	KCNJ1	NP_000211.1
Canis lupus familiaris (dog)	KCNJ1	XP_005619620.1
Gallus gallus (chicken)	KCNJ1	XP_004948024.1

Gene Ontology

The following kcnj1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcnj1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa13200	XM_004916171.3 Latest version!	Xenopus tropicalis potassium channel, inwardly rectifying subfamily J, member 1 (kcnj1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OXa62256	XM_004916171.4 Latest version!	Xenopus tropicalis potassium inwardly rectifying channel subfamily J member 1 (kcnj1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kcnj1 ( XM_004916171.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916228.1 Xenopus tropicalis potassium channel, inwardly rectifying subfamily J, member 1 (kcnj1), mRNA.

Price & Availability↑

CloneID	OXa13200
Clone ID Related Accession (Same CDS sequence)	XM_004916171.3
Accession Version	XM_004916171.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-sensitive inward rectifier potassium channel 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCTT ACGTTCGGAA GAAATTAAAC TTGCATCTGG CAGAGCGCCA ACGACGTAGA 
AGTCGCCTCG TATCTAAGGA TGGCAGGTGT AATATAGAAT TTGGAAATGT GGAAGAGCAA 
TCAAGAGTGG CATTCTTAGC TGATATTTGG ACCACAATCT TAGACCTCAA ATGGAGGTAC 
AAAATGACAG TTTTTATTTC TGCCTTTTTA GGGAGCTGGT TCCTTTTTGG CCTATTGTGG 
TATGCTGTGG CTTATTTGCA CAAAGACCTT CCAGAATTTA ATCCTTCTGT AAATCACACA 
CCCTGTGTGG AAAACATAAA TGGCTTGACC TCCTCTTTTC TATTCTCTTT GGAAACTCAG 
GTGACAATCG GGTATGGATT TCGTTGTGTG ACTGAGCAAT GTGCCACAGC AATATTTTTA 
CTGGTGGTTC AGTCAATTCT GGGGGTAATA ATTAACTCAT TTATGTGTGG AGCTATTCTT 
GCTAAGATTT CAAGACCCAA AAAAAGAGCT AAAACCATCA CCTTTAGCAA AAATGCAGTA 
ATAAGCAAGA GAGGAGGAAA ACTCTGTCTT CTTATCAGAG TGGCAAATCT CAGAAAGAGT 
CTGCTAATTG GGAGTCATAT TTATGGAAAG CTGTTAAAAA CTACTGTGAC TCCAGAAGGA 
GAGACAATCA TACTAGATCA AGTCAACATT GAATTTACAG TCGACGCAGG AAGTGAGAAT 
CTGTTTTTTG TTTCCCCACT AACAATTTAT CACATTATTG ATAAAGATAG TCCTTTTTTT 
GAGATGTCTG CCGAGACTTT ACTGCAGCAT GACTTTGAAC TAGTGGTCTT CCTTGATGGA 
ACTGTCGAGT CCACAAGTGC TACCTGCCAA GTTCGTACAT CCTTCATTCC AGATGAAGTG 
CTCTGGGGAT ACCGTTTTGC ACCTCTTGTG TCCAAGACCA AGGAAGGCAA ATATCGTGTG 
GATTTCTCCA ACTTCAGCAA GACTTTGGAG GTAGAAACAC CCCATTGTGC CTTTTGTCTT 
CAAAATGAGA AAGATGCCAG GGAAAAAAGC AAAAAAGGAT ATGATAATCC AGGATTTGCC 
CTGTCAGAAC CACCAGAAAC CATTGACACT AAAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916228.1
CDS	106..1224
Translation	MLSYVRKKLNLHLAERQRRRSRLVSKDGRCNIEFGNVEEQSRVAFLADIWTTILDLKWRYKMTVFISAFLGSWFLFGLLWYAVAYLHKDLPEFNPSVNHTPCVENINGLTSSFLFSLETQVTIGYGFRCVTEQCATAIFLLVVQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTVTPEGETIILDQVNIEFTVDAGSENLFFVSPLTIYHIIDKDSPFFEMSAETLLQHDFELVVFLDGTVESTSATCQVRTSFIPDEVLWGYRFAPLVSKTKEGKYRVDFSNFSKTLEVETPHCAFCLQNEKDAREKSKKGYDNPGFALSEPPETIDTKM

Target ORF information:

RefSeq Version	XM_004916171.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, inwardly rectifying subfamily J, member 1 (kcnj1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916171.3

ORF Insert Sequence:

ATGTTGTCTT ACGTTCGGAA GAAATTAAAC TTGCATCTGG CAGAGCGCCA ACGACGTAGA 
AGTCGCCTCG TATCTAAGGA TGGCAGGTGT AATATAGAAT TTGGAAATGT GGAAGAGCAA 
TCAAGAGTGG CATTCTTAGC TGATATTTGG ACCACAATCT TAGACCTCAA ATGGAGGTAC 
AAAATGACAG TTTTTATTTC TGCCTTTTTA GGGAGCTGGT TCCTTTTTGG CCTATTGTGG 
TATGCTGTGG CTTATTTGCA CAAAGACCTT CCAGAATTTA ATCCTTCTGT AAATCACACA 
CCCTGTGTGG AAAACATAAA TGGCTTGACC TCCTCTTTTC TATTCTCTTT GGAAACTCAG 
GTGACAATCG GGTATGGATT TCGTTGTGTG ACTGAGCAAT GTGCCACAGC AATATTTTTA 
CTGGTGGTTC AGTCAATTCT GGGGGTAATA ATTAACTCAT TTATGTGTGG AGCTATTCTT 
GCTAAGATTT CAAGACCCAA AAAAAGAGCT AAAACCATCA CCTTTAGCAA AAATGCAGTA 
ATAAGCAAGA GAGGAGGAAA ACTCTGTCTT CTTATCAGAG TGGCAAATCT CAGAAAGAGT 
CTGCTAATTG GGAGTCATAT TTATGGAAAG CTGTTAAAAA CTACTGTGAC TCCAGAAGGA 
GAGACAATCA TACTAGATCA AGTCAACATT GAATTTACAG TCGACGCAGG AAGTGAGAAT 
CTGTTTTTTG TTTCCCCACT AACAATTTAT CACATTATTG ATAAAGATAG TCCTTTTTTT 
GAGATGTCTG CCGAGACTTT ACTGCAGCAT GACTTTGAAC TAGTGGTCTT CCTTGATGGA 
ACTGTCGAGT CCACAAGTGC TACCTGCCAA GTTCGTACAT CCTTCATTCC AGATGAAGTG 
CTCTGGGGAT ACCGTTTTGC ACCTCTTGTG TCCAAGACCA AGGAAGGCAA ATATCGTGTG 
GATTTCTCCA ACTTCAGCAA GACTTTGGAG GTAGAAACAC CCCATTGTGC CTTTTGTCTT 
CAAAATGAGA AAGATGCCAG GGAAAAAAGC AAAAAAGGAT ATGATAATCC AGGATTTGCC 
CTGTCAGAAC CACCAGAAAC CATTGACACT AAAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnj1 ( XM_004916171.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916228.1 Xenopus tropicalis potassium inwardly rectifying channel subfamily J member 1 (kcnj1), mRNA.

Price & Availability↑

CloneID	OXa62256
Clone ID Related Accession (Same CDS sequence)	XM_004916171.4
Accession Version	XM_004916171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-sensitive inward rectifier potassium channel 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCTT ACGTTCGGAA GAAATTAAAC TTGCATCTGG CAGAGCGCCA ACGACGTAGA 
AGTCGCCTCG TATCTAAGGA TGGCAGGTGT AATATAGAAT TTGGAAATGT GGAAGAGCAA 
TCAAGAGTGG CATTCTTAGC TGATATTTGG ACCACAATCT TAGACCTCAA ATGGAGGTAC 
AAAATGACAG TTTTTATTTC TGCCTTTTTA GGGAGCTGGT TCCTTTTTGG CCTATTGTGG 
TATGCTGTGG CTTATTTACA CAAAGACCTT CCAGAATTTA ATCCTTCTGT AAATCACACA 
CCCTGTGTGG AAAACATAAA TGGCTTGACC TCCTCTTTTC TATTCTCTTT GGAAACTCAG 
GTGACAATCG GGTATGGATT TCGTTGTGTG ACTGAGCAAT GTGCCACAGC AATATTTTTA 
CTGGTGGTTC AGTCAATTCT GGGGGTAATA ATTAACTCAT TTATGTGTGG AGCTATTCTT 
GCTAAGATTT CAAGACCCAA AAAAAGAGCT AAAACCATCA CCTTTAGCAA AAATGCAGTA 
ATAAGCAAGA GAGGAGGAAA ACTCTGTCTT CTTATCAGAG TGGCAAATCT CAGAAAGAGT 
CTGCTAATTG GGAGTCATAT TTATGGAAAG CTGTTAAAAA CTACTGTGAC TCCAGAAGGA 
GAGACAATCA TACTAGATCA AGTCAACATT GAATTTACAG TCGACGCAGG AAGTGAGAAT 
CTGTTTTTTG TTTCCCCACT AACAATTTAT CACATTATTG ATAAAGATAG TCCTTTTTTT 
GAGATGTCTG CCGAGACTTT ACTGCAGCAT GACTTTGAAC TAGTGGTCTT CCTTGATGGA 
ACTGTCGAGT CCACAAGTGC TACCTGCCAA GTTCGTACAT CCTTCATTCC AGATGAAGTG 
CTCTGGGGAT ACCGTTTTGC ACCTCTTGTG TCCAAGACCA AGGAAGGCAA ATATCGTGTG 
GATTTCTCCA ACTTCAGCAA GACTTTGGAG GTAGAAACAC CCCATTGTGC CTTTTGTCTT 
CAAAATGAGA AAGATGCCAG GGAAAAAAGC AAAAAAGGAT ATGATAATCC AGGATTTGCC 
CTGTCAGAAC CACCAGAAAC CATTGACACT AAAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916228.1
CDS	118..1236
Translation	MLSYVRKKLNLHLAERQRRRSRLVSKDGRCNIEFGNVEEQSRVAFLADIWTTILDLKWRYKMTVFISAFLGSWFLFGLLWYAVAYLHKDLPEFNPSVNHTPCVENINGLTSSFLFSLETQVTIGYGFRCVTEQCATAIFLLVVQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTVTPEGETIILDQVNIEFTVDAGSENLFFVSPLTIYHIIDKDSPFFEMSAETLLQHDFELVVFLDGTVESTSATCQVRTSFIPDEVLWGYRFAPLVSKTKEGKYRVDFSNFSKTLEVETPHCAFCLQNEKDAREKSKKGYDNPGFALSEPPETIDTKM

Target ORF information:

RefSeq Version	XM_004916171.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium inwardly rectifying channel subfamily J member 1 (kcnj1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916171.4

ORF Insert Sequence:

ATGTTGTCTT ACGTTCGGAA GAAATTAAAC TTGCATCTGG CAGAGCGCCA ACGACGTAGA 
AGTCGCCTCG TATCTAAGGA TGGCAGGTGT AATATAGAAT TTGGAAATGT GGAAGAGCAA 
TCAAGAGTGG CATTCTTAGC TGATATTTGG ACCACAATCT TAGACCTCAA ATGGAGGTAC 
AAAATGACAG TTTTTATTTC TGCCTTTTTA GGGAGCTGGT TCCTTTTTGG CCTATTGTGG 
TATGCTGTGG CTTATTTACA CAAAGACCTT CCAGAATTTA ATCCTTCTGT AAATCACACA 
CCCTGTGTGG AAAACATAAA TGGCTTGACC TCCTCTTTTC TATTCTCTTT GGAAACTCAG 
GTGACAATCG GGTATGGATT TCGTTGTGTG ACTGAGCAAT GTGCCACAGC AATATTTTTA 
CTGGTGGTTC AGTCAATTCT GGGGGTAATA ATTAACTCAT TTATGTGTGG AGCTATTCTT 
GCTAAGATTT CAAGACCCAA AAAAAGAGCT AAAACCATCA CCTTTAGCAA AAATGCAGTA 
ATAAGCAAGA GAGGAGGAAA ACTCTGTCTT CTTATCAGAG TGGCAAATCT CAGAAAGAGT 
CTGCTAATTG GGAGTCATAT TTATGGAAAG CTGTTAAAAA CTACTGTGAC TCCAGAAGGA 
GAGACAATCA TACTAGATCA AGTCAACATT GAATTTACAG TCGACGCAGG AAGTGAGAAT 
CTGTTTTTTG TTTCCCCACT AACAATTTAT CACATTATTG ATAAAGATAG TCCTTTTTTT 
GAGATGTCTG CCGAGACTTT ACTGCAGCAT GACTTTGAAC TAGTGGTCTT CCTTGATGGA 
ACTGTCGAGT CCACAAGTGC TACCTGCCAA GTTCGTACAT CCTTCATTCC AGATGAAGTG 
CTCTGGGGAT ACCGTTTTGC ACCTCTTGTG TCCAAGACCA AGGAAGGCAA ATATCGTGTG 
GATTTCTCCA ACTTCAGCAA GACTTTGGAG GTAGAAACAC CCCATTGTGC CTTTTGTCTT 
CAAAATGAGA AAGATGCCAG GGAAAAAAGC AAAAAAGGAT ATGATAATCC AGGATTTGCC 
CTGTCAGAAC CACCAGAAAC CATTGACACT AAAATGTAG