Kcnj1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj1
Entrez Gene ID	24521
Full Name	potassium voltage-gated channel subfamily J member 1
Synonyms	KAB-1,Kcnj,ROMK1,kir1.1
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 1 Names ATP-sensitive inward rectifier potassium channel 1 ATP-regulated potassium channel ROM-K K+ channel protein inward rectifier K(+) channel Kir1.1 potassium channel, inwardly rectifying subfamily J member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q21
Summary	potassium channel involved in K+ secretion in the renal distal nephron [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001309298.1	NP_001296227.1	ATP-sensitive inward rectifier potassium channel 1 isoform 3
NM_001309301.1	NP_001296230.1	ATP-sensitive inward rectifier potassium channel 1 isoform 3
NM_001309299.1	NP_001296228.1	ATP-sensitive inward rectifier potassium channel 1 isoform 3
NM_001309297.1	NP_001296226.1	ATP-sensitive inward rectifier potassium channel 1 isoform 2
XM_008766047.2	XP_008764269.1	ATP-sensitive inward rectifier potassium channel 1 isoform X2
XM_008766044.2	XP_008764266.1	ATP-sensitive inward rectifier potassium channel 1 isoform X1
NM_017023.2	NP_058719.2	ATP-sensitive inward rectifier potassium channel 1 isoform 1
XM_017595458.1	XP_017450947.1	ATP-sensitive inward rectifier potassium channel 1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KCNJ1	NP_001179136.1
Rattus norvegicus (Norway rat)	Kcnj1	NP_058719.1
	KCNJ1	XP_001113379.1
Danio rerio (zebrafish)	kcnj1b	NP_001092204.1
Xenopus tropicalis (tropical clawed frog)	kcnj1	XP_004916228.1
Pan troglodytes (chimpanzee)	KCNJ1	XP_522246.2
Mus musculus (house mouse)	Kcnj1	NP_001161826.1
Homo sapiens (human)	KCNJ1	NP_000211.1
Canis lupus familiaris (dog)	KCNJ1	XP_005619620.1
Gallus gallus (chicken)	KCNJ1	XP_004948024.1

Gene Ontology
Bibliography

Related articles in PubMed

Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome.
O'Donnell BM, Mackie TD, Subramanya AR, Brodsky JL
The Journal of biological chemistry292(31)12813-12827(2017 08)

Inhibition of ROMK blocks macula densa tubuloglomerular feedback yet causes renal vasoconstriction in anesthetized rats.
Araujo M, Welch WJ, Zhou X, Sullivan K, Walsh S, Pasternak A, Wilcox CS
American journal of physiology. Renal physiology312(6)F1120-F1127(2017 Jun)

Potassium Channel Candidate Genes Predict the Development of Secondary Lymphedema Following Breast Cancer Surgery.
Smoot B, Kober KM, Paul SM, Levine JD, Abrams G, Mastick J, Topp K, Conley YP, Miaskowski CA
Nursing research66(2)85-94(2017 Mar/Apr)

State-dependent network connectivity determines gating in a K+ channel.
Bollepalli MK, Fowler PW, Rapedius M, Shang L, Sansom MS, Tucker SJ, Baukrowitz T
Structure (London, England : 1993)22(7)1037-46(2014 Jul)

Unique mechanism of the interaction between honey bee toxin TPNQ and rKir1.1 potassium channel explored by computational simulations: insights into the relative insensitivity of channel towards animal toxins.
Hu J, Qiu S, Yang F, Cao Z, Li W, Wu Y
PloS one8(7)e67213(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest underlying pathology for some patients with type II Bartter syndrome is linked to stability of ROMK1 in ERAD pathway; using a yeast expression system, cells can be rescued by wild-type (rat) ROMK1 but not by ROMK1 containing any one of four mutations found in (human) type II Bartter syndrome; mutant ROMKs are significantly less stable than wild-type ROMK. (ERAD = endoplasmic reticulum-associated degradation)
Title: Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome.

Blockade of ROMK or Na(+)-K(+)-2Cl(-) transport inhibits tubuloglomerular feedback yet increases renal vascular resistance. The renal vasoconstriction was independent of volume depletion, blood pressure, TGF, or proximal tubule hydrostatic pressure.
Title: Inhibition of ROMK blocks macula densa tubuloglomerular feedback yet causes renal vasoconstriction in anesthetized rats.

These preliminary findings suggest that K channel genes play a role in the development of secondary lymphedema
Title: Potassium Channel Candidate Genes Predict the Development of Secondary Lymphedema Following Breast Cancer Surgery.

Data indicate that mutations in kir1.1 potassium channel preferentially increase pH sensitivity.
Title: State-dependent network connectivity determines gating in a K+ channel.

The TPNQ toxin-rKir1.1 channel complex structure not only revealed their unique interaction mechanism, but also elucidate the relative insensitivity of rKir1.1 channel towards animal toxins.
Title: Unique mechanism of the interaction between honey bee toxin TPNQ and rKir1.1 potassium channel explored by computational simulations: insights into the relative insensitivity of channel towards animal toxins.

The following Kcnj1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42915	NM_001309297.1 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42916	NM_017023.2 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42917	NM_001309299.1 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa42917	NM_001309301.1 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa42917	NM_001309298.1 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa42914	XM_008766044.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42915	XM_017595458.1 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42915	XM_008766047.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj1 ( NM_001309297.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001296226.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa42915
Clone ID Related Accession (Same CDS sequence)	NM_001309297.1 , XM_008766047.2 , XM_017595458.1
Accession Version	NM_001309297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127149.4. On Jun 9, 2015 this sequence version replaced XM_008766046.1. Transcript Variant: This variant (2, also known as ROMK2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29403.1, CK483163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642715 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAAAC ACCTCCGAAG ATGGTTTGTC ACTCACATAT TTGGGCGTTC CCGGCAACGG 
GCAAGGCTGG TCTCTAAAGA AGGAAGATGT AACATCGAGT TTGGCAATGT GGATGCACAG 
TCAAGGTTTA TATTCTTTGT GGACATCTGG ACAACTGTGC TGGACCTGAA ATGGAGGTAC 
AAAATGACCG TGTTCATCAC AGCCTTCTTG GGGAGTTGGT TCCTCTTTGG TCTCCTGTGG 
TATGTCGTAG CGTATGTTCA TAAGGACCTC CCAGAGTTCT ACCCGCCTGA CAACCGCACT 
CCTTGTGTGG AGAACATTAA TGGCATGACT TCAGCCTTTC TGTTTTCTCT AGAGACTCAA 
GTGACCATAG GTTACGGATT CAGGTTTGTG ACAGAACAGT GCGCCACTGC CATTTTCCTG 
CTTATCTTCC AATCTATTCT TGGAGTGATC ATCAATTCCT TCATGTGTGG TGCCATTTTA 
GCCAAGATCT CTAGACCTAA AAAACGTGCT AAAACCATTA CGTTCAGCAA GAATGCGGTG 
ATCAGCAAGC GTGGCGGGAA GCTCTGCCTC CTCATCCGAG TGGCCAATCT TAGGAAGAGC 
CTTCTGATTG GCAGCCACAT ATATGGCAAG CTTCTAAAGA CAACCATCAC TCCTGAAGGC 
GAGACCATCA TTTTGGATCA GACCAACATC AACTTTGTCG TCGACGCTGG CAATGAAAAT 
TTGTTCTTCA TATCCCCACT GACGATCTAC CACATTATTG ACCACAACAG CCCTTTCTTC 
CACATGGCAG CAGAAACTCT TTCCCAACAG GACTTTGAGC TGGTGGTCTT TTTAGATGGC 
ACAGTGGAAT CCACCAGTGC AACCTGCCAG GTCCGCACGT CATACGTCCC AGAGGAGGTA 
CTTTGGGGCT ACCGTTTCGT TCCTATTGTG TCCAAGACCA AGGAAGGGAA ATACCGAGTG 
GATTTTCATA ACTTCGGTAA GACAGTGGAA GTGGAGACCC CTCACTGTGC CATGTGCCTC 
TATAATGAGA AAGATGCCAG GGCCAGGATG AAGAGAGGCT ATGACAACCC TAACTTTGTC 
TTGTCAGAAG TTGATGAAAC GGACGACACC CAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296226.1
CDS	190..1308
Translation	MFKHLRRWFVTHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	NM_001309297.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309297.1

ORF Insert Sequence:

ATGTTCAAAC ACCTCCGAAG ATGGTTTGTC ACTCACATAT TTGGGCGTTC CCGGCAACGG 
GCAAGGCTGG TCTCTAAAGA AGGAAGATGT AACATCGAGT TTGGCAATGT GGATGCACAG 
TCAAGGTTTA TATTCTTTGT GGACATCTGG ACAACTGTGC TGGACCTGAA ATGGAGGTAC 
AAAATGACCG TGTTCATCAC AGCCTTCTTG GGGAGTTGGT TCCTCTTTGG TCTCCTGTGG 
TATGTCGTAG CGTATGTTCA TAAGGACCTC CCAGAGTTCT ACCCGCCTGA CAACCGCACT 
CCTTGTGTGG AGAACATTAA TGGCATGACT TCAGCCTTTC TGTTTTCTCT AGAGACTCAA 
GTGACCATAG GTTACGGATT CAGGTTTGTG ACAGAACAGT GCGCCACTGC CATTTTCCTG 
CTTATCTTCC AATCTATTCT TGGAGTGATC ATCAATTCCT TCATGTGTGG TGCCATTTTA 
GCCAAGATCT CTAGACCTAA AAAACGTGCT AAAACCATTA CGTTCAGCAA GAATGCGGTG 
ATCAGCAAGC GTGGCGGGAA GCTCTGCCTC CTCATCCGAG TGGCCAATCT TAGGAAGAGC 
CTTCTGATTG GCAGCCACAT ATATGGCAAG CTTCTAAAGA CAACCATCAC TCCTGAAGGC 
GAGACCATCA TTTTGGATCA GACCAACATC AACTTTGTCG TCGACGCTGG CAATGAAAAT 
TTGTTCTTCA TATCCCCACT GACGATCTAC CACATTATTG ACCACAACAG CCCTTTCTTC 
CACATGGCAG CAGAAACTCT TTCCCAACAG GACTTTGAGC TGGTGGTCTT TTTAGATGGC 
ACAGTGGAAT CCACCAGTGC AACCTGCCAG GTCCGCACGT CATACGTCCC AGAGGAGGTA 
CTTTGGGGCT ACCGTTTCGT TCCTATTGTG TCCAAGACCA AGGAAGGGAA ATACCGAGTG 
GATTTTCATA ACTTCGGTAA GACAGTGGAA GTGGAGACCC CTCACTGTGC CATGTGCCTC 
TATAATGAGA AAGATGCCAG GGCCAGGATG AAGAGAGGCT ATGACAACCC TAACTTTGTC 
TTGTCAGAAG TTGATGAAAC GGACGACACC CAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( NM_017023.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058719.2 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa42916
Clone ID Related Accession (Same CDS sequence)	NM_017023.2
Accession Version	NM_017023.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127149.4. On Jun 9, 2015 this sequence version replaced NM_017023.1. Transcript Variant: This variant (1, also known as ROMK1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X72341.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCTT CGGAACGGAG TGTGTTCAGA GTGCTGATCA GGGCACTGAC AGAAAGGATG 
TTCAAACACC TCCGAAGATG GTTTGTCACT CACATATTTG GGCGTTCCCG GCAACGGGCA 
AGGCTGGTCT CTAAAGAAGG AAGATGTAAC ATCGAGTTTG GCAATGTGGA TGCACAGTCA 
AGGTTTATAT TCTTTGTGGA CATCTGGACA ACTGTGCTGG ACCTGAAATG GAGGTACAAA 
ATGACCGTGT TCATCACAGC CTTCTTGGGG AGTTGGTTCC TCTTTGGTCT CCTGTGGTAT 
GTCGTAGCGT ATGTTCATAA GGACCTCCCA GAGTTCTACC CGCCTGACAA CCGCACTCCT 
TGTGTGGAGA ACATTAATGG CATGACTTCA GCCTTTCTGT TTTCTCTAGA GACTCAAGTG 
ACCATAGGTT ACGGATTCAG GTTTGTGACA GAACAGTGCG CCACTGCCAT TTTCCTGCTT 
ATCTTCCAAT CTATTCTTGG AGTGATCATC AATTCCTTCA TGTGTGGTGC CATTTTAGCC 
AAGATCTCTA GACCTAAAAA ACGTGCTAAA ACCATTACGT TCAGCAAGAA TGCGGTGATC 
AGCAAGCGTG GCGGGAAGCT CTGCCTCCTC ATCCGAGTGG CCAATCTTAG GAAGAGCCTT 
CTGATTGGCA GCCACATATA TGGCAAGCTT CTAAAGACAA CCATCACTCC TGAAGGCGAG 
ACCATCATTT TGGATCAGAC CAACATCAAC TTTGTCGTCG ACGCTGGCAA TGAAAATTTG 
TTCTTCATAT CCCCACTGAC GATCTACCAC ATTATTGACC ACAACAGCCC TTTCTTCCAC 
ATGGCAGCAG AAACTCTTTC CCAACAGGAC TTTGAGCTGG TGGTCTTTTT AGATGGCACA 
GTGGAATCCA CCAGTGCAAC CTGCCAGGTC CGCACGTCAT ACGTCCCAGA GGAGGTACTT 
TGGGGCTACC GTTTCGTTCC TATTGTGTCC AAGACCAAGG AAGGGAAATA CCGAGTGGAT 
TTTCATAACT TCGGTAAGAC AGTGGAAGTG GAGACCCCTC ACTGTGCCAT GTGCCTCTAT 
AATGAGAAAG ATGCCAGGGC CAGGATGAAG AGAGGCTATG ACAACCCTAA CTTTGTCTTG 
TCAGAAGTTG ATGAAACGGA CGACACCCAG ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058719.2
CDS	150..1325
Translation	MGASERSVFRVLIRALTERMFKHLRRWFVTHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	NM_017023.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017023.2

ORF Insert Sequence:

ATGGGCGCTT CGGAACGGAG TGTGTTCAGA GTGCTGATCA GGGCACTGAC AGAAAGGATG 
TTCAAACACC TCCGAAGATG GTTTGTCACT CACATATTTG GGCGTTCCCG GCAACGGGCA 
AGGCTGGTCT CTAAAGAAGG AAGATGTAAC ATCGAGTTTG GCAATGTGGA TGCACAGTCA 
AGGTTTATAT TCTTTGTGGA CATCTGGACA ACTGTGCTGG ACCTGAAATG GAGGTACAAA 
ATGACCGTGT TCATCACAGC CTTCTTGGGG AGTTGGTTCC TCTTTGGTCT CCTGTGGTAT 
GTCGTAGCGT ATGTTCATAA GGACCTCCCA GAGTTCTACC CGCCTGACAA CCGCACTCCT 
TGTGTGGAGA ACATTAATGG CATGACTTCA GCCTTTCTGT TTTCTCTAGA GACTCAAGTG 
ACCATAGGTT ACGGATTCAG GTTTGTGACA GAACAGTGCG CCACTGCCAT TTTCCTGCTT 
ATCTTCCAAT CTATTCTTGG AGTGATCATC AATTCCTTCA TGTGTGGTGC CATTTTAGCC 
AAGATCTCTA GACCTAAAAA ACGTGCTAAA ACCATTACGT TCAGCAAGAA TGCGGTGATC 
AGCAAGCGTG GCGGGAAGCT CTGCCTCCTC ATCCGAGTGG CCAATCTTAG GAAGAGCCTT 
CTGATTGGCA GCCACATATA TGGCAAGCTT CTAAAGACAA CCATCACTCC TGAAGGCGAG 
ACCATCATTT TGGATCAGAC CAACATCAAC TTTGTCGTCG ACGCTGGCAA TGAAAATTTG 
TTCTTCATAT CCCCACTGAC GATCTACCAC ATTATTGACC ACAACAGCCC TTTCTTCCAC 
ATGGCAGCAG AAACTCTTTC CCAACAGGAC TTTGAGCTGG TGGTCTTTTT AGATGGCACA 
GTGGAATCCA CCAGTGCAAC CTGCCAGGTC CGCACGTCAT ACGTCCCAGA GGAGGTACTT 
TGGGGCTACC GTTTCGTTCC TATTGTGTCC AAGACCAAGG AAGGGAAATA CCGAGTGGAT 
TTTCATAACT TCGGTAAGAC AGTGGAAGTG GAGACCCCTC ACTGTGCCAT GTGCCTCTAT 
AATGAGAAAG ATGCCAGGGC CAGGATGAAG AGAGGCTATG ACAACCCTAA CTTTGTCTTG 
TCAGAAGTTG ATGAAACGGA CGACACCCAG ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( NM_001309298.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001296227.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa42917
Clone ID Related Accession (Same CDS sequence)	NM_001309298.1 , NM_001309301.1 , NM_001309299.1
Accession Version	NM_001309298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127149.4. Transcript Variant: This variant (3, also known as ROMK6.1) contains two alternate exons in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Variants 3, 4 and 5 encode the same protein (isoform 3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF081368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642749, SAMN02642762 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296227.1
CDS	615..1271
Translation	MCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	NM_001309298.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309298.1

ORF Insert Sequence:

ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( NM_001309301.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001296230.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 5, mRNA.

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CloneID	ORa42917
Clone ID Related Accession (Same CDS sequence)	NM_001309298.1 , NM_001309301.1 , NM_001309299.1
Accession Version	NM_001309301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127149.4. Transcript Variant: This variant (5, also known as ROMK1.1) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Variants 3, 4 and 5 encode the same protein (isoform 3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF081365.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296230.1
CDS	493..1149
Translation	MCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	NM_001309301.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309301.1

ORF Insert Sequence:

ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( NM_001309299.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001296228.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 4, mRNA.

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CloneID	ORa42917
Clone ID Related Accession (Same CDS sequence)	NM_001309298.1 , NM_001309301.1 , NM_001309299.1
Accession Version	NM_001309299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127149.4. Transcript Variant: This variant (4, also known as ROMK3.1) contains three alternate exons in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Variants 3, 4 and 5 encode the same protein (isoform 3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF081367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642747 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296228.1
CDS	598..1254
Translation	MCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	NM_001309299.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309299.1

ORF Insert Sequence:

ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( XM_008766044.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764266.1 Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42914
Clone ID Related Accession (Same CDS sequence)	XM_008766044.2
Accession Version	XM_008766044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCTG AGCTTTCCAT TCCTTCTATT CCTACAGGAG TGGCAGGACT CAGTAAGATC 
AGGGCACTGA CAGAAAGGAT GTTCAAACAC CTCCGAAGAT GGTTTGTCAC TCACATATTT 
GGGCGTTCCC GGCAACGGGC AAGGCTGGTC TCTAAAGAAG GAAGATGTAA CATCGAGTTT 
GGCAATGTGG ATGCACAGTC AAGGTTTATA TTCTTTGTGG ACATCTGGAC AACTGTGCTG 
GACCTGAAAT GGAGGTACAA AATGACCGTG TTCATCACAG CCTTCTTGGG GAGTTGGTTC 
CTCTTTGGTC TCCTGTGGTA TGTCGTAGCG TATGTTCATA AGGACCTCCC AGAGTTCTAC 
CCGCCTGACA ACCGCACTCC TTGTGTGGAG AACATTAATG GCATGACTTC AGCCTTTCTG 
TTTTCTCTAG AGACTCAAGT GACCATAGGT TACGGATTCA GGTTTGTGAC AGAACAGTGC 
GCCACTGCCA TTTTCCTGCT TATCTTCCAA TCTATTCTTG GAGTGATCAT CAATTCCTTC 
ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764266.1
CDS	260..1456
Translation	MVSELSIPSIPTGVAGLSKIRALTERMFKHLRRWFVTHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	XM_008766044.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766044.2

ORF Insert Sequence:

ATGGTGTCTG AGCTTTCCAT TCCTTCTATT CCTACAGGAG TGGCAGGACT CAGTAAGATC 
AGGGCACTGA CAGAAAGGAT GTTCAAACAC CTCCGAAGAT GGTTTGTCAC TCACATATTT 
GGGCGTTCCC GGCAACGGGC AAGGCTGGTC TCTAAAGAAG GAAGATGTAA CATCGAGTTT 
GGCAATGTGG ATGCACAGTC AAGGTTTATA TTCTTTGTGG ACATCTGGAC AACTGTGCTG 
GACCTGAAAT GGAGGTACAA AATGACCGTG TTCATCACAG CCTTCTTGGG GAGTTGGTTC 
CTCTTTGGTC TCCTGTGGTA TGTCGTAGCG TATGTTCATA AGGACCTCCC AGAGTTCTAC 
CCGCCTGACA ACCGCACTCC TTGTGTGGAG AACATTAATG GCATGACTTC AGCCTTTCTG 
TTTTCTCTAG AGACTCAAGT GACCATAGGT TACGGATTCA GGTTTGTGAC AGAACAGTGC 
GCCACTGCCA TTTTCCTGCT TATCTTCCAA TCTATTCTTG GAGTGATCAT CAATTCCTTC 
ATGTGTGGTG CCATTTTAGC CAAGATCTCT AGACCTAAAA AACGTGCTAA AACCATTACG 
TTCAGCAAGA ATGCGGTGAT CAGCAAGCGT GGCGGGAAGC TCTGCCTCCT CATCCGAGTG 
GCCAATCTTA GGAAGAGCCT TCTGATTGGC AGCCACATAT ATGGCAAGCT TCTAAAGACA 
ACCATCACTC CTGAAGGCGA GACCATCATT TTGGATCAGA CCAACATCAA CTTTGTCGTC 
GACGCTGGCA ATGAAAATTT GTTCTTCATA TCCCCACTGA CGATCTACCA CATTATTGAC 
CACAACAGCC CTTTCTTCCA CATGGCAGCA GAAACTCTTT CCCAACAGGA CTTTGAGCTG 
GTGGTCTTTT TAGATGGCAC AGTGGAATCC ACCAGTGCAA CCTGCCAGGT CCGCACGTCA 
TACGTCCCAG AGGAGGTACT TTGGGGCTAC CGTTTCGTTC CTATTGTGTC CAAGACCAAG 
GAAGGGAAAT ACCGAGTGGA TTTTCATAAC TTCGGTAAGA CAGTGGAAGT GGAGACCCCT 
CACTGTGCCA TGTGCCTCTA TAATGAGAAA GATGCCAGGG CCAGGATGAA GAGAGGCTAT 
GACAACCCTA ACTTTGTCTT GTCAGAAGTT GATGAAACGG ACGACACCCA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( XM_008766047.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764269.1 Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa42915
Clone ID Related Accession (Same CDS sequence)	NM_001309297.1 , XM_008766047.2 , XM_017595458.1
Accession Version	XM_008766047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAAC ACCTCCGAAG ATGGTTTGTC ACTCACATAT TTGGGCGTTC CCGGCAACGG 
GCAAGGCTGG TCTCTAAAGA AGGAAGATGT AACATCGAGT TTGGCAATGT GGATGCACAG 
TCAAGGTTTA TATTCTTTGT GGACATCTGG ACAACTGTGC TGGACCTGAA ATGGAGGTAC 
AAAATGACCG TGTTCATCAC AGCCTTCTTG GGGAGTTGGT TCCTCTTTGG TCTCCTGTGG 
TATGTCGTAG CGTATGTTCA TAAGGACCTC CCAGAGTTCT ACCCGCCTGA CAACCGCACT 
CCTTGTGTGG AGAACATTAA TGGCATGACT TCAGCCTTTC TGTTTTCTCT AGAGACTCAA 
GTGACCATAG GTTACGGATT CAGGTTTGTG ACAGAACAGT GCGCCACTGC CATTTTCCTG 
CTTATCTTCC AATCTATTCT TGGAGTGATC ATCAATTCCT TCATGTGTGG TGCCATTTTA 
GCCAAGATCT CTAGACCTAA AAAACGTGCT AAAACCATTA CGTTCAGCAA GAATGCGGTG 
ATCAGCAAGC GTGGCGGGAA GCTCTGCCTC CTCATCCGAG TGGCCAATCT TAGGAAGAGC 
CTTCTGATTG GCAGCCACAT ATATGGCAAG CTTCTAAAGA CAACCATCAC TCCTGAAGGC 
GAGACCATCA TTTTGGATCA GACCAACATC AACTTTGTCG TCGACGCTGG CAATGAAAAT 
TTGTTCTTCA TATCCCCACT GACGATCTAC CACATTATTG ACCACAACAG CCCTTTCTTC 
CACATGGCAG CAGAAACTCT TTCCCAACAG GACTTTGAGC TGGTGGTCTT TTTAGATGGC 
ACAGTGGAAT CCACCAGTGC AACCTGCCAG GTCCGCACGT CATACGTCCC AGAGGAGGTA 
CTTTGGGGCT ACCGTTTCGT TCCTATTGTG TCCAAGACCA AGGAAGGGAA ATACCGAGTG 
GATTTTCATA ACTTCGGTAA GACAGTGGAA GTGGAGACCC CTCACTGTGC CATGTGCCTC 
TATAATGAGA AAGATGCCAG GGCCAGGATG AAGAGAGGCT ATGACAACCC TAACTTTGTC 
TTGTCAGAAG TTGATGAAAC GGACGACACC CAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764269.1
CDS	341..1459
Translation	MFKHLRRWFVTHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	XM_008766047.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766047.2

ORF Insert Sequence:

ATGTTCAAAC ACCTCCGAAG ATGGTTTGTC ACTCACATAT TTGGGCGTTC CCGGCAACGG 
GCAAGGCTGG TCTCTAAAGA AGGAAGATGT AACATCGAGT TTGGCAATGT GGATGCACAG 
TCAAGGTTTA TATTCTTTGT GGACATCTGG ACAACTGTGC TGGACCTGAA ATGGAGGTAC 
AAAATGACCG TGTTCATCAC AGCCTTCTTG GGGAGTTGGT TCCTCTTTGG TCTCCTGTGG 
TATGTCGTAG CGTATGTTCA TAAGGACCTC CCAGAGTTCT ACCCGCCTGA CAACCGCACT 
CCTTGTGTGG AGAACATTAA TGGCATGACT TCAGCCTTTC TGTTTTCTCT AGAGACTCAA 
GTGACCATAG GTTACGGATT CAGGTTTGTG ACAGAACAGT GCGCCACTGC CATTTTCCTG 
CTTATCTTCC AATCTATTCT TGGAGTGATC ATCAATTCCT TCATGTGTGG TGCCATTTTA 
GCCAAGATCT CTAGACCTAA AAAACGTGCT AAAACCATTA CGTTCAGCAA GAATGCGGTG 
ATCAGCAAGC GTGGCGGGAA GCTCTGCCTC CTCATCCGAG TGGCCAATCT TAGGAAGAGC 
CTTCTGATTG GCAGCCACAT ATATGGCAAG CTTCTAAAGA CAACCATCAC TCCTGAAGGC 
GAGACCATCA TTTTGGATCA GACCAACATC AACTTTGTCG TCGACGCTGG CAATGAAAAT 
TTGTTCTTCA TATCCCCACT GACGATCTAC CACATTATTG ACCACAACAG CCCTTTCTTC 
CACATGGCAG CAGAAACTCT TTCCCAACAG GACTTTGAGC TGGTGGTCTT TTTAGATGGC 
ACAGTGGAAT CCACCAGTGC AACCTGCCAG GTCCGCACGT CATACGTCCC AGAGGAGGTA 
CTTTGGGGCT ACCGTTTCGT TCCTATTGTG TCCAAGACCA AGGAAGGGAA ATACCGAGTG 
GATTTTCATA ACTTCGGTAA GACAGTGGAA GTGGAGACCC CTCACTGTGC CATGTGCCTC 
TATAATGAGA AAGATGCCAG GGCCAGGATG AAGAGAGGCT ATGACAACCC TAACTTTGTC 
TTGTCAGAAG TTGATGAAAC GGACGACACC CAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj1 ( XM_017595458.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450947.1 Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa42915
Clone ID Related Accession (Same CDS sequence)	NM_001309297.1 , XM_008766047.2 , XM_017595458.1
Accession Version	XM_017595458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-sensitive inward rectifier potassium channel 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAAC ACCTCCGAAG ATGGTTTGTC ACTCACATAT TTGGGCGTTC CCGGCAACGG 
GCAAGGCTGG TCTCTAAAGA AGGAAGATGT AACATCGAGT TTGGCAATGT GGATGCACAG 
TCAAGGTTTA TATTCTTTGT GGACATCTGG ACAACTGTGC TGGACCTGAA ATGGAGGTAC 
AAAATGACCG TGTTCATCAC AGCCTTCTTG GGGAGTTGGT TCCTCTTTGG TCTCCTGTGG 
TATGTCGTAG CGTATGTTCA TAAGGACCTC CCAGAGTTCT ACCCGCCTGA CAACCGCACT 
CCTTGTGTGG AGAACATTAA TGGCATGACT TCAGCCTTTC TGTTTTCTCT AGAGACTCAA 
GTGACCATAG GTTACGGATT CAGGTTTGTG ACAGAACAGT GCGCCACTGC CATTTTCCTG 
CTTATCTTCC AATCTATTCT TGGAGTGATC ATCAATTCCT TCATGTGTGG TGCCATTTTA 
GCCAAGATCT CTAGACCTAA AAAACGTGCT AAAACCATTA CGTTCAGCAA GAATGCGGTG 
ATCAGCAAGC GTGGCGGGAA GCTCTGCCTC CTCATCCGAG TGGCCAATCT TAGGAAGAGC 
CTTCTGATTG GCAGCCACAT ATATGGCAAG CTTCTAAAGA CAACCATCAC TCCTGAAGGC 
GAGACCATCA TTTTGGATCA GACCAACATC AACTTTGTCG TCGACGCTGG CAATGAAAAT 
TTGTTCTTCA TATCCCCACT GACGATCTAC CACATTATTG ACCACAACAG CCCTTTCTTC 
CACATGGCAG CAGAAACTCT TTCCCAACAG GACTTTGAGC TGGTGGTCTT TTTAGATGGC 
ACAGTGGAAT CCACCAGTGC AACCTGCCAG GTCCGCACGT CATACGTCCC AGAGGAGGTA 
CTTTGGGGCT ACCGTTTCGT TCCTATTGTG TCCAAGACCA AGGAAGGGAA ATACCGAGTG 
GATTTTCATA ACTTCGGTAA GACAGTGGAA GTGGAGACCC CTCACTGTGC CATGTGCCTC 
TATAATGAGA AAGATGCCAG GGCCAGGATG AAGAGAGGCT ATGACAACCC TAACTTTGTC 
TTGTCAGAAG TTGATGAAAC GGACGACACC CAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450947.1
CDS	2344..3462
Translation	MFKHLRRWFVTHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

Target ORF information:

RefSeq Version	XM_017595458.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily J member 1 (Kcnj1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595458.1

ORF Insert Sequence:

ATGTTCAAAC ACCTCCGAAG ATGGTTTGTC ACTCACATAT TTGGGCGTTC CCGGCAACGG 
GCAAGGCTGG TCTCTAAAGA AGGAAGATGT AACATCGAGT TTGGCAATGT GGATGCACAG 
TCAAGGTTTA TATTCTTTGT GGACATCTGG ACAACTGTGC TGGACCTGAA ATGGAGGTAC 
AAAATGACCG TGTTCATCAC AGCCTTCTTG GGGAGTTGGT TCCTCTTTGG TCTCCTGTGG 
TATGTCGTAG CGTATGTTCA TAAGGACCTC CCAGAGTTCT ACCCGCCTGA CAACCGCACT 
CCTTGTGTGG AGAACATTAA TGGCATGACT TCAGCCTTTC TGTTTTCTCT AGAGACTCAA 
GTGACCATAG GTTACGGATT CAGGTTTGTG ACAGAACAGT GCGCCACTGC CATTTTCCTG 
CTTATCTTCC AATCTATTCT TGGAGTGATC ATCAATTCCT TCATGTGTGG TGCCATTTTA 
GCCAAGATCT CTAGACCTAA AAAACGTGCT AAAACCATTA CGTTCAGCAA GAATGCGGTG 
ATCAGCAAGC GTGGCGGGAA GCTCTGCCTC CTCATCCGAG TGGCCAATCT TAGGAAGAGC 
CTTCTGATTG GCAGCCACAT ATATGGCAAG CTTCTAAAGA CAACCATCAC TCCTGAAGGC 
GAGACCATCA TTTTGGATCA GACCAACATC AACTTTGTCG TCGACGCTGG CAATGAAAAT 
TTGTTCTTCA TATCCCCACT GACGATCTAC CACATTATTG ACCACAACAG CCCTTTCTTC 
CACATGGCAG CAGAAACTCT TTCCCAACAG GACTTTGAGC TGGTGGTCTT TTTAGATGGC 
ACAGTGGAAT CCACCAGTGC AACCTGCCAG GTCCGCACGT CATACGTCCC AGAGGAGGTA 
CTTTGGGGCT ACCGTTTCGT TCCTATTGTG TCCAAGACCA AGGAAGGGAA ATACCGAGTG 
GATTTTCATA ACTTCGGTAA GACAGTGGAA GTGGAGACCC CTCACTGTGC CATGTGCCTC 
TATAATGAGA AAGATGCCAG GGCCAGGATG AAGAGAGGCT ATGACAACCC TAACTTTGTC 
TTGTCAGAAG TTGATGAAAC GGACGACACC CAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome.
The Journal of biological chemistry292(31)12813-12827(2017 08)
O'Donnell BM,Mackie TD,Subramanya AR,Brodsky JL

Inhibition of ROMK blocks macula densa tubuloglomerular feedback yet causes renal vasoconstriction in anesthetized rats.
American journal of physiology. Renal physiology312(6)F1120-F1127(2017 Jun)
Araujo M,Welch WJ,Zhou X,Sullivan K,Walsh S,Pasternak A,Wilcox CS

Potassium Channel Candidate Genes Predict the Development of Secondary Lymphedema Following Breast Cancer Surgery.
Nursing research66(2)85-94(2017 Mar/Apr)
Smoot B,Kober KM,Paul SM,Levine JD,Abrams G,Mastick J,Topp K,Conley YP,Miaskowski CA

State-dependent network connectivity determines gating in a K+ channel.
Structure (London, England : 1993)22(7)1037-46(2014 Jul)
Bollepalli MK,Fowler PW,Rapedius M,Shang L,Sansom MS,Tucker SJ,Baukrowitz T

Unique mechanism of the interaction between honey bee toxin TPNQ and rKir1.1 potassium channel explored by computational simulations: insights into the relative insensitivity of channel towards animal toxins.
PloS one8(7)e67213(2013)
Hu J,Qiu S,Yang F,Cao Z,Li W,Wu Y

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Kcnj1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

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