LOC100486501 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100486501
Entrez Gene ID	100486501
Full Name	uncharacterized LOC100486501
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096844.1	XP_017952333.1	uncharacterized protein LOC100486501 isoform X6
XM_018096846.1	XP_017952335.1	uncharacterized protein LOC100486501 isoform X8
XM_018096848.1	XP_017952337.1	uncharacterized protein LOC100486501 isoform X10
XM_018096850.1	XP_017952339.1	uncharacterized protein LOC100486501 isoform X12
XM_018096856.1	XP_017952345.1	uncharacterized protein LOC100486501 isoform X18
XM_031891110.1	XP_031746970.1	uncharacterized protein LOC100486501 isoform X4
XM_031891112.1	XP_031746972.1	uncharacterized protein LOC100486501 isoform X6
XM_031891119.1	XP_031746979.1	uncharacterized protein LOC100486501 isoform X12
XM_031891121.1	XP_031746981.1	uncharacterized protein LOC100486501 isoform X14
XM_031891127.1	XP_031746987.1	uncharacterized protein LOC100486501 isoform X19
XM_031891129.1	XP_031746989.1	uncharacterized protein LOC100486501 isoform X21
XM_031891134.1	XP_031746994.1	uncharacterized protein LOC100486501 isoform X26
XM_031891136.1	XP_031746996.1	uncharacterized protein LOC100486501 isoform X28
XM_018096840.1	XP_017952329.1	uncharacterized protein LOC100486501 isoform X2
XM_018096841.1	XP_017952330.1	uncharacterized protein LOC100486501 isoform X3
XM_018096852.1	XP_017952341.1	uncharacterized protein LOC100486501 isoform X14
XM_031891109.1	XP_031746969.1	uncharacterized protein LOC100486501 isoform X3
XM_031891111.1	XP_031746971.1	uncharacterized protein LOC100486501 isoform X5
XM_031891114.1	XP_031746974.1	uncharacterized protein LOC100486501 isoform X8
XM_031891118.1	XP_031746978.1	uncharacterized protein LOC100486501 isoform X11
XM_031891120.1	XP_031746980.1	uncharacterized protein LOC100486501 isoform X13
XM_031891124.1	XP_031746984.1	uncharacterized protein LOC100486501 isoform X17
XM_031891125.1	XP_031746985.1	uncharacterized protein LOC100486501 isoform X18
XM_031891132.1	XP_031746992.1	uncharacterized protein LOC100486501 isoform X24
XM_018096839.1	XP_017952328.1	uncharacterized protein LOC100486501 isoform X1
XM_018096842.1	XP_017952331.1	uncharacterized protein LOC100486501 isoform X4
XM_018096843.1	XP_017952332.1	uncharacterized protein LOC100486501 isoform X5
XM_018096853.1	XP_017952342.1	uncharacterized protein LOC100486501 isoform X15
XM_031891107.1	XP_031746967.1	uncharacterized protein LOC100486501 isoform X1
XM_031891108.1	XP_031746968.1	uncharacterized protein LOC100486501 isoform X2
XM_031891116.1	XP_031746976.1	uncharacterized protein LOC100486501 isoform X9
XM_031891131.1	XP_031746991.1	uncharacterized protein LOC100486501 isoform X23
XM_031891133.1	XP_031746993.1	uncharacterized protein LOC100486501 isoform X25
XM_018096847.1	XP_017952336.1	uncharacterized protein LOC100486501 isoform X9
XM_018096849.1	XP_017952338.1	uncharacterized protein LOC100486501 isoform X11
XM_018096851.1	XP_017952340.1	uncharacterized protein LOC100486501 isoform X13
XM_018096854.1	XP_017952343.1	uncharacterized protein LOC100486501 isoform X16
XM_018096855.1	XP_017952344.1	uncharacterized protein LOC100486501 isoform X17
XM_018096857.1	XP_017952346.1	uncharacterized protein LOC100486501 isoform X19
XM_031891106.1	XP_031746966.1	uncharacterized protein LOC100486501 isoform X1
XM_031891113.1	XP_031746973.1	uncharacterized protein LOC100486501 isoform X7
XM_031891122.1	XP_031746982.1	uncharacterized protein LOC100486501 isoform X15
XM_031891130.1	XP_031746990.1	uncharacterized protein LOC100486501 isoform X22
XM_031891135.1	XP_031746995.1	uncharacterized protein LOC100486501 isoform X27
XM_031891137.1	XP_031746997.1	uncharacterized protein LOC100486501 isoform X29
XM_018096838.1	XP_017952327.1	uncharacterized protein LOC100486501 isoform X1
XM_018096845.1	XP_017952334.1	uncharacterized protein LOC100486501 isoform X7
XM_031891117.1	XP_031746977.1	uncharacterized protein LOC100486501 isoform X10
XM_031891123.1	XP_031746983.1	uncharacterized protein LOC100486501 isoform X16
XM_031891128.1	XP_031746988.1	uncharacterized protein LOC100486501 isoform X20

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100495557	XP_002943469.2
Xenopus tropicalis (tropical clawed frog)	LOC100488631	XP_002938807.2
Xenopus tropicalis (tropical clawed frog)	LOC100489032	XP_002944426.2
Xenopus tropicalis (tropical clawed frog)	LOC100490667	XP_002939041.2
Xenopus tropicalis (tropical clawed frog)	LOC100492862	XP_002943188.2
Xenopus tropicalis (tropical clawed frog)	LOC100488315	XP_002938805.2
Xenopus tropicalis (tropical clawed frog)	LOC101731187	XP_004917392.1
Xenopus tropicalis (tropical clawed frog)	LOC101732413	XP_004919378.1
Xenopus tropicalis (tropical clawed frog)	LOC101731995	XP_004919373.1
Xenopus tropicalis (tropical clawed frog)	LOC101732174	XP_004919375.1
Xenopus tropicalis (tropical clawed frog)	LOC101734608	XP_004917562.1
Xenopus tropicalis (tropical clawed frog)	LOC100487362	XP_002938799.2
Xenopus tropicalis (tropical clawed frog)	LOC101732346	XP_004919377.1
Xenopus tropicalis (tropical clawed frog)	LOC100486657	XP_002942132.2
Xenopus tropicalis (tropical clawed frog)	LOC101734538	XP_004917561.1
Xenopus tropicalis (tropical clawed frog)	LOC100488475	XP_004917390.1
Xenopus tropicalis (tropical clawed frog)	LOC100491005	XP_002939043.2
Xenopus tropicalis (tropical clawed frog)	LOC100487685	XP_002938801.2
Xenopus tropicalis (tropical clawed frog)	LOC101731928	XP_004919372.1
Xenopus tropicalis (tropical clawed frog)	LOC101731872	XP_004919371.1
Xenopus tropicalis (tropical clawed frog)	LOC101732060	XP_004919374.1
Xenopus tropicalis (tropical clawed frog)	LOC101732231	XP_004919376.1
Xenopus tropicalis (tropical clawed frog)	LOC100486501	XP_002942131.2
Xenopus tropicalis (tropical clawed frog)	LOC100490323	XP_002939039.1
Xenopus tropicalis (tropical clawed frog)	LOC101731813	XP_004919370.1
Xenopus tropicalis (tropical clawed frog)	LOC101731120	XP_004917391.1
Xenopus tropicalis (tropical clawed frog)	LOC101734712	XP_004917563.1
Xenopus tropicalis (tropical clawed frog)	LOC101734780	XP_004917564.1

Gene Ontology

The following LOC100486501 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100486501 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa33634	XM_018096838.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa33634	XM_018096839.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46446	XM_018096840.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46447	XM_018096841.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46448	XM_018096842.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46449	XM_018096843.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46450	XM_018096844.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46451	XM_018096845.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46452	XM_018096846.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46453	XM_018096847.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46454	XM_018096848.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46455	XM_018096849.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46456	XM_018096850.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46457	XM_018096851.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46458	XM_018096852.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46459	XM_018096853.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46460	XM_018096854.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46461	XM_018096855.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46462	XM_018096856.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa46463	XM_018096857.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa64376	XM_031891107.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64376	XM_031891106.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64377	XM_031891108.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64378	XM_031891109.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64379	XM_031891110.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64380	XM_031891111.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64381	XM_031891112.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64382	XM_031891113.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64383	XM_031891114.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64384	XM_031891116.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64385	XM_031891117.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64386	XM_031891118.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64387	XM_031891119.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64388	XM_031891120.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64389	XM_031891121.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64390	XM_031891122.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64391	XM_031891123.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64392	XM_031891124.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64393	XM_031891125.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64394	XM_031891127.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64395	XM_031891128.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64396	XM_031891129.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64397	XM_031891130.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64398	XM_031891131.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64399	XM_031891132.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64400	XM_031891133.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64401	XM_031891134.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64402	XM_031891135.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64403	XM_031891136.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa64404	XM_031891137.1 Latest version!	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100486501 ( XM_018096839.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952328.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33634
Clone ID Related Accession (Same CDS sequence)	XM_018096839.1 , XM_018096838.1
Accession Version	XM_018096839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGATCTCT GTTAAGGCCT CATCCTGAGT GTCCAGTTCA GTTTTCGGGC 
CCAGCCCTTA TAAAGGATGT TAGTGAGCTG GAGAGAGTGC AGTGCCATTC AACTAAACTG 
GTAAAAGAGA TGGGAGAATT TAGCTATGAG GAAAGACAAT CAAGTGCAAG CTTTCCCCTG 
CAGCTCCCAT TCAAACAGGG AGTTTCCAAA GACTTGGAGG ACATCACTTC AACCTATGTC 
TATAGCCCTG GGATTCATAC TGCATGCATA GGCAATAACA ATGTTCTGAA GTCTGTGTAT 
ACGTATAAAG GTTTGCAAAC TGACAGCCTT CATGCTGATA TCATGAATGG TAATGAGTGC 
AAATGCACAG AAGAGACGGC CAATCATCTT AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952328.1
CDS	307..10251
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096839.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096839.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGATCTCT GTTAAGGCCT CATCCTGAGT GTCCAGTTCA GTTTTCGGGC 
CCAGCCCTTA TAAAGGATGT TAGTGAGCTG GAGAGAGTGC AGTGCCATTC AACTAAACTG 
GTAAAAGAGA TGGGAGAATT TAGCTATGAG GAAAGACAAT CAAGTGCAAG CTTTCCCCTG 
CAGCTCCCAT TCAAACAGGG AGTTTCCAAA GACTTGGAGG ACATCACTTC AACCTATGTC 
TATAGCCCTG GGATTCATAC TGCATGCATA GGCAATAACA ATGTTCTGAA GTCTGTGTAT 
ACGTATAAAG GTTTGCAAAC TGACAGCCTT CATGCTGATA TCATGAATGG TAATGAGTGC 
AAATGCACAG AAGAGACGGC CAATCATCTT AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096838.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952327.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X1, mRNA.

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CloneID	OXa33634
Clone ID Related Accession (Same CDS sequence)	XM_018096839.1 , XM_018096838.1
Accession Version	XM_018096838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGATCTCT GTTAAGGCCT CATCCTGAGT GTCCAGTTCA GTTTTCGGGC 
CCAGCCCTTA TAAAGGATGT TAGTGAGCTG GAGAGAGTGC AGTGCCATTC AACTAAACTG 
GTAAAAGAGA TGGGAGAATT TAGCTATGAG GAAAGACAAT CAAGTGCAAG CTTTCCCCTG 
CAGCTCCCAT TCAAACAGGG AGTTTCCAAA GACTTGGAGG ACATCACTTC AACCTATGTC 
TATAGCCCTG GGATTCATAC TGCATGCATA GGCAATAACA ATGTTCTGAA GTCTGTGTAT 
ACGTATAAAG GTTTGCAAAC TGACAGCCTT CATGCTGATA TCATGAATGG TAATGAGTGC 
AAATGCACAG AAGAGACGGC CAATCATCTT AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952327.1
CDS	452..10396
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096838.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096838.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGATCTCT GTTAAGGCCT CATCCTGAGT GTCCAGTTCA GTTTTCGGGC 
CCAGCCCTTA TAAAGGATGT TAGTGAGCTG GAGAGAGTGC AGTGCCATTC AACTAAACTG 
GTAAAAGAGA TGGGAGAATT TAGCTATGAG GAAAGACAAT CAAGTGCAAG CTTTCCCCTG 
CAGCTCCCAT TCAAACAGGG AGTTTCCAAA GACTTGGAGG ACATCACTTC AACCTATGTC 
TATAGCCCTG GGATTCATAC TGCATGCATA GGCAATAACA ATGTTCTGAA GTCTGTGTAT 
ACGTATAAAG GTTTGCAAAC TGACAGCCTT CATGCTGATA TCATGAATGG TAATGAGTGC 
AAATGCACAG AAGAGACGGC CAATCATCTT AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096840.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952329.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X3, mRNA.

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CloneID	OXa46446
Clone ID Related Accession (Same CDS sequence)	XM_018096840.1
Accession Version	XM_018096840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952329.1
CDS	452..10384
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096840.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096840.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096841.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952330.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X4, mRNA.

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CloneID	OXa46447
Clone ID Related Accession (Same CDS sequence)	XM_018096841.1
Accession Version	XM_018096841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGG ATAAAGAGCA TATTAAAGAC 
TCTATTACTT ACAGTGAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGATGTTCT GATGAATTCT AGGAGAAATA TGCAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCGCAG AGACATTCTC AGAATTTAGA AAATGTTGAT 
CAGTCTGCAT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATATAAAAA TGTCTCTGGA GTCTCAATCT CAGCCTCTGC ATCATCTGCA 
TCATCCTCTT CTGGATACCT TGCATCCCCA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGTCAGT CTGCTTCTAA TAAGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGGA GTCTCCATCT CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCC AATGGAAAAC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC TCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGTGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTTTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA TTCTCAGGGC AAAGAGCAAA CTAAAGATGT TATTACATAC 
AGTGAAGCTC AGAGAAATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCTGA TGCTCCAAGC TTTCCATCAT CCATCTGTTC CCCTCCTGTA 
TATCCTGTGT CTCCAACAGA AAACAGTCAA CAGGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCGCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952330.1
CDS	452..10339
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096841.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096841.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGG ATAAAGAGCA TATTAAAGAC 
TCTATTACTT ACAGTGAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGATGTTCT GATGAATTCT AGGAGAAATA TGCAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCGCAG AGACATTCTC AGAATTTAGA AAATGTTGAT 
CAGTCTGCAT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATATAAAAA TGTCTCTGGA GTCTCAATCT CAGCCTCTGC ATCATCTGCA 
TCATCCTCTT CTGGATACCT TGCATCCCCA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGTCAGT CTGCTTCTAA TAAGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGGA GTCTCCATCT CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCC AATGGAAAAC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC TCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGTGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTTTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA TTCTCAGGGC AAAGAGCAAA CTAAAGATGT TATTACATAC 
AGTGAAGCTC AGAGAAATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCTGA TGCTCCAAGC TTTCCATCAT CCATCTGTTC CCCTCCTGTA 
TATCCTGTGT CTCCAACAGA AAACAGTCAA CAGGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCGCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096842.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952331.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X5, mRNA.

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CloneID	OXa46448
Clone ID Related Accession (Same CDS sequence)	XM_018096842.1
Accession Version	XM_018096842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGTGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTTTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA TTCTCAGGGC AAAGAGCAAA CTAAAGATGT TATTACATAC 
AGTGAAGCTC AGAGAAATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCTGA TGCTCCAAGC TTTCCATCAT CCATCTGTTC CCCTCCTGTA 
TATCCTGTGT CTCCAACAGA AAACAGTCAA CAGGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCGCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952331.1
CDS	452..10339
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096842.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096842.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGTGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTTTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA TTCTCAGGGC AAAGAGCAAA CTAAAGATGT TATTACATAC 
AGTGAAGCTC AGAGAAATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCTGA TGCTCCAAGC TTTCCATCAT CCATCTGTTC CCCTCCTGTA 
TATCCTGTGT CTCCAACAGA AAACAGTCAA CAGGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCGCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096843.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952332.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X6, mRNA.

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CloneID	OXa46449
Clone ID Related Accession (Same CDS sequence)	XM_018096843.1
Accession Version	XM_018096843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGATGTTCT GATGAATTCT AGGAGAAATA TGCAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCGCAG AGACATTCTC AGAATTTAGA AAATGTTGAT 
CAGTCTGCAT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATATAAAAA TGTCTCTGGA GTCTCAATCT CAGCCTCTGC ATCATCTGCA 
TCATCCTCTT CTGGATACCT TGCATCCCCA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGTCAGT CTGCTTCTAA TAAGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGGA GTCTCCATCT CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCC AATGGAAAAC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC TCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGTGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTTTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA TTCTCAGGGC AAAGAGCAAA CTAAAGATGT TATTACATAC 
AGTGAAGCTC AGAGAAATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCTGA TGCTCCAAGC TTTCCATCAT CCATCTGTTC CCCTCCTGTA 
TATCCTGTGT CTCCAACAGA AAACAGTCAA CAGGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCGCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952332.1
CDS	452..10339
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096843.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096843.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGATGTTCT GATGAATTCT AGGAGAAATA TGCAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCGCAG AGACATTCTC AGAATTTAGA AAATGTTGAT 
CAGTCTGCAT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATATAAAAA TGTCTCTGGA GTCTCAATCT CAGCCTCTGC ATCATCTGCA 
TCATCCTCTT CTGGATACCT TGCATCCCCA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGTCAGT CTGCTTCTAA TAAGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGGA GTCTCCATCT CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCC AATGGAAAAC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC TCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGTGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTTTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA TTCTCAGGGC AAAGAGCAAA CTAAAGATGT TATTACATAC 
AGTGAAGCTC AGAGAAATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCTGA TGCTCCAAGC TTTCCATCAT CCATCTGTTC CCCTCCTGTA 
TATCCTGTGT CTCCAACAGA AAACAGTCAA CAGGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCGCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096844.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952333.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X7, mRNA.

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CloneID	OXa46450
Clone ID Related Accession (Same CDS sequence)	XM_018096844.1
Accession Version	XM_018096844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952333.1
CDS	452..10339
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096844.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096844.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGTGAAGCC 
AAGATAAGTA CTCGGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACATCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096845.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952334.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X8, mRNA.

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CloneID	OXa46451
Clone ID Related Accession (Same CDS sequence)	XM_018096845.1
Accession Version	XM_018096845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952334.1
CDS	452..10339
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096845.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096845.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGAAGCATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCC CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC GGCCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096846.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952335.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X9, mRNA.

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CloneID	OXa46452
Clone ID Related Accession (Same CDS sequence)	XM_018096846.1
Accession Version	XM_018096846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGAACAAAG ATACAAACAG AGCTTCGACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAACAGAG CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGAT ACAACCAAAG ATACAAACGG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACGGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAGGATAC AAACAGAGAT ACAACCAAAG ATACAAACAG AGATACAACC AAGGATACAA 
ACAGAGATAC TACCGGAGTT ACGAACAACG ATAAAAACAG ACACAAAATC AGAAATACAA 
ACAGACATAA AGATACAAAC TGAGGAAAAA ATAAAAGCAG GAGGAGAAAT CCAAGTTATT 
GGGCGTTTAG GGGAGACTGT CACACTTCAG TGCCCTCTGA ATTCACCAGC AAAAATCTCA 
TGGATGTTTG ATGATAATGG GGAAAAAATT CTCCTGGCTG AATACAGAGA CCAGAAATTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT GGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TATTTATGTT CTCACAGTGT TGGGAAGAGG AGACATCATT 
CTGCCGGTGA CTGGCCTTGT AGGGGGGAAT GTCACACTGC CAACCTATCT GACTCTGCCA 
GCTGCCATCG AAAAAATCTC ATGGATGTTT CATGTCAATG GGAAGCGAAT ACACCTGGCT 
GAATTCAGAC ACCAGAAGTT TATCACAGTT AATAAGAAAT ATTTCTTAAA CCGCCTTGAA 
AAACCTAATG ATGGAACCGC ATTAAGGATC AGAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTCATTT TAATGGACGA AAAAGAGACA TTTCTTACAT TCTAGCCGTT 
TTCGAGCCTA TCCCAACACC AGTGATAAAA GTGACGTTTG ATGAGAATTC CACTGATCTC 
TGCCATCTTC ATTGTTCTGT CCCATCAAAC TCACCAACCC TGTCCTACAG TTGGACCTAC 
AGAGATACTG GCACTGACAG ACTATATGCC AATGGCAGCA CAATCACTTT ATCGCTGAAG 
GATAAGGCAT TGGGGGCAGA ATTTACCTGC TGGGTACAGA ATCCTGCTCA CAGGAACAAT 
GCATCAGTCC CTTTAAAGTC ACTTCATGGG AGACAAACAG GAATACAAAC TGAGATACAA 
CCAGGGATAC AAAGAAAGAT ACAACCAGAG ATACAAACAA AGATACAAAA AGAGATACGA 
CCAGACATAA AAGCAGAGAT TCAACCAAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAA CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACAACCAGGG 
ATACAAACAG AGATACAACC AGATATAAAA GCAGAGATAA AACCAAAGAT ACAAGCAGGG 
ATACACACTG AGCTACAACC AAAGATAAAA ACAGAGATAC GACCAGGGAC ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AAATAAAAAC AGAGTTACGA CCAGAGATAA AAACAGAGCT TCCACCAGAA 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTTC GACCAGAGAT AAAAACAGAG 
ATACGACCAG AGATGAAAAC AGAGCTACGA CCAGAGATAA AAACAGAAAT ACAACCAGGG 
ATACGTACAG ATATACAACC AGGGATACAA ACAGATCTAC GAACAGAGAT ACAAACAGAT 
CTACGAACAG AGATACAAAA AAAGATACAA ACAGAAATAC TAACAGAGGT GCAAACAGAC 
ATAAAGAGAC AAACAAAGGC AAAAATAAAA GCAGGAGGAG ACATCCAGGT GATTGGCCGT 
GTAGGGGAGA CTGTCACACT GCAGTGCCCT CTGAATCTAC CAACAAGAAT TTCATGGACG 
TATGATGATA ATGGGGAAAA AATACTCCTG GCTGAATACA GAGACCAGAA ATTTATCAGA 
GTGAATAACA ACTACTTCAT CAACCGGCTA GAAGGGTCTA ACAGTGGCAC CGCATTACTG 
ATCAGGGAGC TAAAAAGAGA GGATAGTGGG GTCTTCACTG CTCACATTAC TGTTAATGGA 
AGCGCTACAG ACATTAGTTA TATACTGAAT GTACTGGGAA GGGAAGACAT CATTCTGCCG 
GTGACTGGCC TTGTAGGGGG GACTGTCACA CTGCCAACCT ATCTGACTCT GCCAGCTGCC 
ATCGAAAGAA TCTCATGGAT GTTTCATGAC AAAGGCAAAA AAATACACCT GGCTGAATTA 
AGAAACCATA AGTTTAGCAG ACTGAACAAG AATTATTTCT ACAACCGCCT TGAAAAATCT 
AATGATGGAA CCGCATTACA GATCAGAGAG CTGAGAATGG AGGATTCTGG GATCTTCACT 
GCACACATTC ATGTTAATGG ACAAAAAAGA GACATTTCTT ATATTCTAGC TGTGTTCGAG 
CCTATCCCAA CACCAGTAAT AGAAGTGACA TCTGATGAGA ATTTCCCTGA TCTCTGCCAT 
CTTCATTGTT CTGTCCCATC AAACTCACCA ACCCTGTCCT ACAGTTGGAC CTACAGAGAT 
ACTGGCACTG ACAGACTATA TGCCAATGGC AGCACAATCA CTGTATCACT GAAGGATAAG 
GCATTGGGGG CAGAATTTAC CTGCTGGGTA CAGAATCCTG CTCACAGGAA CAGTGTGTCA 
GTCGTTTTAA AGTCATATGG TGGGAGACAA ACAGGAATAA AACCAAAGAT ACGACCAGAG 
ATACAACAAA AAATACAAAT GGAGACACGA CCAGAGATAA AAACAGAGAT ACGACCAGGG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGCTAC AACCAGAGAT AAAAACAGAA 
ATGCAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACAGAGAT ACGACCAGGG 
ATAAAAACAG AGATACAACC AGAGATAAAA ACAGAAATAC AGCCAGGGAT AAAAACAGAG 
AAACAACCAG GGATACTAAC GGAGATACGA CCAGGGATAA AAACAGAGAT GCGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AGAGAGAGAC ACAAAGAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATAA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACCGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATAGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG CCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGATGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC GGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
ACAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAT GGATACAAAA AGAGATACAG 
CCGGAGATAC AAACAGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GAAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GAAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GAAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGAAT ACAAAAAGAG ACACGAAGAA AGATACATAA AGAGATAGAA 
CCAAATATAC AAACAGAGAT AGAAAAAGAA ATGCAACCAG ACATACGACC AGAAATACAT 
ACAGAGATAG AAAAAGATAT TCAACCAGAG ATACAATCAG AAACACCGAG AGAGGCACAA 
ATAAAAGGAC CGAAAAAAGA TTCTCGTATA CTGACAGTAA TAATTGCAGT TTCGATACTG 
TGCGGAATAT TTGTTGGTAT AGCTCTTTAT TATGGAATAA GACGACGACT AAAGGTTAAA 
AATCAGTCTA ATTGCACTAT TACATACAGT GAAGTTCTGA CAAGTACTCA GGATAAAGAG 
CATATTAAAG ACTCTATTAC TTACAGTGAA GTTCAGATAA ATACTCAGGA TAAAGTGCAT 
ACTAAAGAGA CTATTGCATA CAGTGAATTT CAGGGAAATT CTAGGAGAAA TATGCAGGGC 
AAAGAAGAAC CTGATTATAA TGATTATACT GTTACATTCA GTGAAGGTCA GAGAAATACA 
CAGGATAAAG AGCATATTAA AGACTCTATT ACTTACAGTG AAGTTCAGAT AAATACTCAG 
GATAAAGTGC ATACTAAAGA CACTATTACA TACAGTGAAG TTCAGATGAA TTCTAGGAGA 
AATATGCAGG GCAAAGAAGA ATCTGATTAT AATGATTATA CTGTTACATT CAGTGAAGGT 
CAGAGAAATA CACAGAATAA AAAACAGTCT GATGATACTA TTATATACAG TAAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA AGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGATGT TCTGATGAAT 
TCTAGGAGAA ATATGCAGGA TAAAGAGCAA ACTAAAGACA CTATTACATA CACTGAAGCG 
CAGAGACATT CTCAGAATTT AGAAAATGTT GATCAGTCTG CATCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATATAA AAATGTCTCT 
GGAGTCTCAA TCTCAGCCTC TGCATCATCT GCATCATCCT CTTCTGGATA CCTTGCATCC 
CCAATGGAAA GCAGTGTTAA GAATTTAGAA AATGTTGGTC AGTCTGCTTC TAATAAGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GGAGTCTCCA TCTCAGCCTC TGCATCGTCC TCTTCTGGAT ACCTTGCATC CCCAATGGAA 
AACAGTGTTA AGGATAAAGA GCAAACTAAA GACACTATTA CATACACTGA AGCTCAGAGA 
AATTCTCAGG ATAAAGAACA AACTAAAGAA ACTATTACAT ACAGGGAAAG CCAGATAAAT 
ACTCAGAATT TAGAAAATGT TGGCCGGTCT GCCTCTAACA AGCAAAATGG GATGAGCAGT 
GTATCTGGTG ATCATCTTGG ATACTCTGGG TCAGGTCTAT ATGGCGCCTT TTTACATCAG 
ACCAGATCTG CAGGATTATC CGAAGCTACA ACTCTATACA AAAATGTCTC AGGAGGCTCC 
ATCTCAGCCT CTGCATCATC CTCTTCTGGA TACCTTGCAT CCTCAATGGA AAGCAGTGTT 
AAGGATAAAG AGCAAACCAA AGACACTTTT ACATACATTG AAGCTCAGAT AAATTCTCAG 
GGCAAAGAGC AAACTAAAGA TGTTATTACA TACAGTGAAG CTCAGAGAAA TTTTCAGGAT 
AAAGAGCAAC CTAAAGAGAG TATTACATGT AGTGAAGTTT ATAGAAGTAC TCAGGATAAA 
GAGCAAACTA AAGACACAAT TACATACAGT AAAGTTCAGA GAAATATGCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTAGGAAACA CTGGACCAAG TCTGTATGGG GCTTCTTTAG CAGGATTATC TGATGCTCCA 
AGCTTTCCAT CATCCATCTG TTCCCCTCCT GTATATCCTG TGTCTCCAAC AGAAAACAGT 
CAACAGGATA AAAAGCAAAC TAAAGACTCT ATTACCTACA GTGTTGGTCA GATGAATACT 
CAGGATAAAA AGCAAACTCT ATTAACCTAC AGTGTTGGTC AGATAAAGTC TCAGGATATA 
AAGCAAACTA AAGACACTGT TACATATAGA GAAGGCCACA TAAACGCGCA GGATGAACAG 
CAAATGAAAG ACTCCATTAC ATACAGTGAA GCCAAGATAA GTACTCGGAA TCTAGAAAAT 
GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT GTGATGAGCA GTGTATCTGG GGATCGTCTT 
GGATACTTTG GAGCAAGTCT GTATGGGACA TCTTCACATC TGCCCTGGCT TGCTGGATTA 
ATTGAAGCTC CAAGTCTATG TAACACTTGC TCCAGAGTCT CCATCCCAAT CTCAGCATTA 
TGTGCCTGTG CCTCTTCTGG ATCCCATATG TCCCATTGGG ATAGCAGTCA TAAGGAGAAC 
AATCAAACTA AAGACATGAT TACATACAGT GAAGTTCAGA CAAGTATGCA GGATGAACAA 
CAAGCTAAAG GCACTATTAC ATACAGTGAT GGTCAGGTAA AAACTCATGT GTTTGCAGAA 
CAAAAGTCCA ATCACCAAGG CACCAGAAGC ATCAGACAAG AAGGTGGTCA GATGTCAGCG 
CTGATCAAGA ATAGCGAAAT ATCTGAAGAC AATATTACAG ATGATATAAG ATCTCTGTTA 
AGGCCTCATC CTGAGTGTCC AGTTCAGTTT TCGGGCCCAG CCCTTATAAA GGATGTTAGT 
GAGCTGGAGA GAGTGCAGTG CCATTCAACT AAACTGGTAA AAGAGATGGG AGAATTTAGC 
TATGAGGAAA GACAATCAAG TGCAAGCTTT CCCCTGCAGC TCCCATTCAA ACAGGGAGTT 
TCCAAAGACT TGGAGGACAT CACTTCAACC TATGTCTATA GCCCTGGGAT TCATACTGCA 
TGCATAGGCA ATAACAATGT TCTGAAGTCT GTGTATACGT ATAAAGGTTT GCAAACTGAC 
AGCCTTCATG CTGATATCAT GAATGGTAAT GAGTGCAAAT GCACAGAAGA GACGGCCAAT 
CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952335.1
CDS	452..10312
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096846.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096846.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGAACAAAG ATACAAACAG AGCTTCGACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAACAGAG CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGAT ACAACCAAAG ATACAAACGG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACGGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAGGATAC AAACAGAGAT ACAACCAAAG ATACAAACAG AGATACAACC AAGGATACAA 
ACAGAGATAC TACCGGAGTT ACGAACAACG ATAAAAACAG ACACAAAATC AGAAATACAA 
ACAGACATAA AGATACAAAC TGAGGAAAAA ATAAAAGCAG GAGGAGAAAT CCAAGTTATT 
GGGCGTTTAG GGGAGACTGT CACACTTCAG TGCCCTCTGA ATTCACCAGC AAAAATCTCA 
TGGATGTTTG ATGATAATGG GGAAAAAATT CTCCTGGCTG AATACAGAGA CCAGAAATTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT GGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TATTTATGTT CTCACAGTGT TGGGAAGAGG AGACATCATT 
CTGCCGGTGA CTGGCCTTGT AGGGGGGAAT GTCACACTGC CAACCTATCT GACTCTGCCA 
GCTGCCATCG AAAAAATCTC ATGGATGTTT CATGTCAATG GGAAGCGAAT ACACCTGGCT 
GAATTCAGAC ACCAGAAGTT TATCACAGTT AATAAGAAAT ATTTCTTAAA CCGCCTTGAA 
AAACCTAATG ATGGAACCGC ATTAAGGATC AGAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTCATTT TAATGGACGA AAAAGAGACA TTTCTTACAT TCTAGCCGTT 
TTCGAGCCTA TCCCAACACC AGTGATAAAA GTGACGTTTG ATGAGAATTC CACTGATCTC 
TGCCATCTTC ATTGTTCTGT CCCATCAAAC TCACCAACCC TGTCCTACAG TTGGACCTAC 
AGAGATACTG GCACTGACAG ACTATATGCC AATGGCAGCA CAATCACTTT ATCGCTGAAG 
GATAAGGCAT TGGGGGCAGA ATTTACCTGC TGGGTACAGA ATCCTGCTCA CAGGAACAAT 
GCATCAGTCC CTTTAAAGTC ACTTCATGGG AGACAAACAG GAATACAAAC TGAGATACAA 
CCAGGGATAC AAAGAAAGAT ACAACCAGAG ATACAAACAA AGATACAAAA AGAGATACGA 
CCAGACATAA AAGCAGAGAT TCAACCAAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAA CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACAACCAGGG 
ATACAAACAG AGATACAACC AGATATAAAA GCAGAGATAA AACCAAAGAT ACAAGCAGGG 
ATACACACTG AGCTACAACC AAAGATAAAA ACAGAGATAC GACCAGGGAC ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AAATAAAAAC AGAGTTACGA CCAGAGATAA AAACAGAGCT TCCACCAGAA 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTTC GACCAGAGAT AAAAACAGAG 
ATACGACCAG AGATGAAAAC AGAGCTACGA CCAGAGATAA AAACAGAAAT ACAACCAGGG 
ATACGTACAG ATATACAACC AGGGATACAA ACAGATCTAC GAACAGAGAT ACAAACAGAT 
CTACGAACAG AGATACAAAA AAAGATACAA ACAGAAATAC TAACAGAGGT GCAAACAGAC 
ATAAAGAGAC AAACAAAGGC AAAAATAAAA GCAGGAGGAG ACATCCAGGT GATTGGCCGT 
GTAGGGGAGA CTGTCACACT GCAGTGCCCT CTGAATCTAC CAACAAGAAT TTCATGGACG 
TATGATGATA ATGGGGAAAA AATACTCCTG GCTGAATACA GAGACCAGAA ATTTATCAGA 
GTGAATAACA ACTACTTCAT CAACCGGCTA GAAGGGTCTA ACAGTGGCAC CGCATTACTG 
ATCAGGGAGC TAAAAAGAGA GGATAGTGGG GTCTTCACTG CTCACATTAC TGTTAATGGA 
AGCGCTACAG ACATTAGTTA TATACTGAAT GTACTGGGAA GGGAAGACAT CATTCTGCCG 
GTGACTGGCC TTGTAGGGGG GACTGTCACA CTGCCAACCT ATCTGACTCT GCCAGCTGCC 
ATCGAAAGAA TCTCATGGAT GTTTCATGAC AAAGGCAAAA AAATACACCT GGCTGAATTA 
AGAAACCATA AGTTTAGCAG ACTGAACAAG AATTATTTCT ACAACCGCCT TGAAAAATCT 
AATGATGGAA CCGCATTACA GATCAGAGAG CTGAGAATGG AGGATTCTGG GATCTTCACT 
GCACACATTC ATGTTAATGG ACAAAAAAGA GACATTTCTT ATATTCTAGC TGTGTTCGAG 
CCTATCCCAA CACCAGTAAT AGAAGTGACA TCTGATGAGA ATTTCCCTGA TCTCTGCCAT 
CTTCATTGTT CTGTCCCATC AAACTCACCA ACCCTGTCCT ACAGTTGGAC CTACAGAGAT 
ACTGGCACTG ACAGACTATA TGCCAATGGC AGCACAATCA CTGTATCACT GAAGGATAAG 
GCATTGGGGG CAGAATTTAC CTGCTGGGTA CAGAATCCTG CTCACAGGAA CAGTGTGTCA 
GTCGTTTTAA AGTCATATGG TGGGAGACAA ACAGGAATAA AACCAAAGAT ACGACCAGAG 
ATACAACAAA AAATACAAAT GGAGACACGA CCAGAGATAA AAACAGAGAT ACGACCAGGG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGCTAC AACCAGAGAT AAAAACAGAA 
ATGCAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACAGAGAT ACGACCAGGG 
ATAAAAACAG AGATACAACC AGAGATAAAA ACAGAAATAC AGCCAGGGAT AAAAACAGAG 
AAACAACCAG GGATACTAAC GGAGATACGA CCAGGGATAA AAACAGAGAT GCGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AGAGAGAGAC ACAAAGAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATAA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACCGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATAGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG CCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGATGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC GGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
ACAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAT GGATACAAAA AGAGATACAG 
CCGGAGATAC AAACAGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GAAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GAAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GAAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGAAT ACAAAAAGAG ACACGAAGAA AGATACATAA AGAGATAGAA 
CCAAATATAC AAACAGAGAT AGAAAAAGAA ATGCAACCAG ACATACGACC AGAAATACAT 
ACAGAGATAG AAAAAGATAT TCAACCAGAG ATACAATCAG AAACACCGAG AGAGGCACAA 
ATAAAAGGAC CGAAAAAAGA TTCTCGTATA CTGACAGTAA TAATTGCAGT TTCGATACTG 
TGCGGAATAT TTGTTGGTAT AGCTCTTTAT TATGGAATAA GACGACGACT AAAGGTTAAA 
AATCAGTCTA ATTGCACTAT TACATACAGT GAAGTTCTGA CAAGTACTCA GGATAAAGAG 
CATATTAAAG ACTCTATTAC TTACAGTGAA GTTCAGATAA ATACTCAGGA TAAAGTGCAT 
ACTAAAGAGA CTATTGCATA CAGTGAATTT CAGGGAAATT CTAGGAGAAA TATGCAGGGC 
AAAGAAGAAC CTGATTATAA TGATTATACT GTTACATTCA GTGAAGGTCA GAGAAATACA 
CAGGATAAAG AGCATATTAA AGACTCTATT ACTTACAGTG AAGTTCAGAT AAATACTCAG 
GATAAAGTGC ATACTAAAGA CACTATTACA TACAGTGAAG TTCAGATGAA TTCTAGGAGA 
AATATGCAGG GCAAAGAAGA ATCTGATTAT AATGATTATA CTGTTACATT CAGTGAAGGT 
CAGAGAAATA CACAGAATAA AAAACAGTCT GATGATACTA TTATATACAG TAAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA AGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGATGT TCTGATGAAT 
TCTAGGAGAA ATATGCAGGA TAAAGAGCAA ACTAAAGACA CTATTACATA CACTGAAGCG 
CAGAGACATT CTCAGAATTT AGAAAATGTT GATCAGTCTG CATCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATATAA AAATGTCTCT 
GGAGTCTCAA TCTCAGCCTC TGCATCATCT GCATCATCCT CTTCTGGATA CCTTGCATCC 
CCAATGGAAA GCAGTGTTAA GAATTTAGAA AATGTTGGTC AGTCTGCTTC TAATAAGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GGAGTCTCCA TCTCAGCCTC TGCATCGTCC TCTTCTGGAT ACCTTGCATC CCCAATGGAA 
AACAGTGTTA AGGATAAAGA GCAAACTAAA GACACTATTA CATACACTGA AGCTCAGAGA 
AATTCTCAGG ATAAAGAACA AACTAAAGAA ACTATTACAT ACAGGGAAAG CCAGATAAAT 
ACTCAGAATT TAGAAAATGT TGGCCGGTCT GCCTCTAACA AGCAAAATGG GATGAGCAGT 
GTATCTGGTG ATCATCTTGG ATACTCTGGG TCAGGTCTAT ATGGCGCCTT TTTACATCAG 
ACCAGATCTG CAGGATTATC CGAAGCTACA ACTCTATACA AAAATGTCTC AGGAGGCTCC 
ATCTCAGCCT CTGCATCATC CTCTTCTGGA TACCTTGCAT CCTCAATGGA AAGCAGTGTT 
AAGGATAAAG AGCAAACCAA AGACACTTTT ACATACATTG AAGCTCAGAT AAATTCTCAG 
GGCAAAGAGC AAACTAAAGA TGTTATTACA TACAGTGAAG CTCAGAGAAA TTTTCAGGAT 
AAAGAGCAAC CTAAAGAGAG TATTACATGT AGTGAAGTTT ATAGAAGTAC TCAGGATAAA 
GAGCAAACTA AAGACACAAT TACATACAGT AAAGTTCAGA GAAATATGCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTAGGAAACA CTGGACCAAG TCTGTATGGG GCTTCTTTAG CAGGATTATC TGATGCTCCA 
AGCTTTCCAT CATCCATCTG TTCCCCTCCT GTATATCCTG TGTCTCCAAC AGAAAACAGT 
CAACAGGATA AAAAGCAAAC TAAAGACTCT ATTACCTACA GTGTTGGTCA GATGAATACT 
CAGGATAAAA AGCAAACTCT ATTAACCTAC AGTGTTGGTC AGATAAAGTC TCAGGATATA 
AAGCAAACTA AAGACACTGT TACATATAGA GAAGGCCACA TAAACGCGCA GGATGAACAG 
CAAATGAAAG ACTCCATTAC ATACAGTGAA GCCAAGATAA GTACTCGGAA TCTAGAAAAT 
GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT GTGATGAGCA GTGTATCTGG GGATCGTCTT 
GGATACTTTG GAGCAAGTCT GTATGGGACA TCTTCACATC TGCCCTGGCT TGCTGGATTA 
ATTGAAGCTC CAAGTCTATG TAACACTTGC TCCAGAGTCT CCATCCCAAT CTCAGCATTA 
TGTGCCTGTG CCTCTTCTGG ATCCCATATG TCCCATTGGG ATAGCAGTCA TAAGGAGAAC 
AATCAAACTA AAGACATGAT TACATACAGT GAAGTTCAGA CAAGTATGCA GGATGAACAA 
CAAGCTAAAG GCACTATTAC ATACAGTGAT GGTCAGGTAA AAACTCATGT GTTTGCAGAA 
CAAAAGTCCA ATCACCAAGG CACCAGAAGC ATCAGACAAG AAGGTGGTCA GATGTCAGCG 
CTGATCAAGA ATAGCGAAAT ATCTGAAGAC AATATTACAG ATGATATAAG ATCTCTGTTA 
AGGCCTCATC CTGAGTGTCC AGTTCAGTTT TCGGGCCCAG CCCTTATAAA GGATGTTAGT 
GAGCTGGAGA GAGTGCAGTG CCATTCAACT AAACTGGTAA AAGAGATGGG AGAATTTAGC 
TATGAGGAAA GACAATCAAG TGCAAGCTTT CCCCTGCAGC TCCCATTCAA ACAGGGAGTT 
TCCAAAGACT TGGAGGACAT CACTTCAACC TATGTCTATA GCCCTGGGAT TCATACTGCA 
TGCATAGGCA ATAACAATGT TCTGAAGTCT GTGTATACGT ATAAAGGTTT GCAAACTGAC 
AGCCTTCATG CTGATATCAT GAATGGTAAT GAGTGCAAAT GCACAGAAGA GACGGCCAAT 
CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096847.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952336.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X10, mRNA.

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CloneID	OXa46453
Clone ID Related Accession (Same CDS sequence)	XM_018096847.1
Accession Version	XM_018096847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC TGATGCTCCA AGCTTTCCAT CATCCATCTG TTCCCCTCCT 
GTATATCCTG TGTCTCCAAC AGAAAACAGT CAACAGGATA AAAAGCAAAC TAAAGACTCT 
ATTACCTACA GTGTTGGTCA GATGAATACT CAGGATAAAA AGCAAACTCT ATTAACCTAC 
AGTGTTGGTC AGATAAAGTC TCAGGATATA AAGCAAACTA AAGACACTGT TACATATAGA 
GAAGGCCACA TAAACGCGCA GGATGAACAG CAAATGAAAG ACTCCATTAC ATACAGTGAA 
GCCAAGATAA GTACTCGGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCGTCTT GGATACTTTG GAGCAAGTCT GTATGGGACA 
TCTTCACATC TGCCCTGGCT TGCTGGATTA ATTGAAGCTC CAAGTCTATG TAACACTTGC 
TCCAGAGTCT CCATCCCAAT CTCAGCATTA TGTGCCTGTG CCTCTTCTGG ATCCCATATG 
TCCCATTGGG ATAGCAGTCA TAAGGAGAAC AATCAAACTA AAGACATGAT TACATACAGT 
GAAGTTCAGA CAAGTATGCA GGATGAACAA CAAGCTAAAG GCACTATTAC ATACAGTGAT 
GGTCAGGTAA AAACTCATGT GTTTGCAGAA CAAAAGTCCA ATCACCAAGG CACCAGAAGC 
ATCAGACAAG AAGGTGGTCA GATGTCAGCG CTGATCAAGA ATAGCGAAAT ATCTGAAGAC 
AATATTACAG ATGATATAAG ATCTCTGTTA AGGCCTCATC CTGAGTGTCC AGTTCAGTTT 
TCGGGCCCAG CCCTTATAAA GGATGTTAGT GAGCTGGAGA GAGTGCAGTG CCATTCAACT 
AAACTGGTAA AAGAGATGGG AGAATTTAGC TATGAGGAAA GACAATCAAG TGCAAGCTTT 
CCCCTGCAGC TCCCATTCAA ACAGGGAGTT TCCAAAGACT TGGAGGACAT CACTTCAACC 
TATGTCTATA GCCCTGGGAT TCATACTGCA TGCATAGGCA ATAACAATGT TCTGAAGTCT 
GTGTATACGT ATAAAGGTTT GCAAACTGAC AGCCTTCATG CTGATATCAT GAATGGTAAT 
GAGTGCAAAT GCACAGAAGA GACGGCCAAT CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952336.1
CDS	452..10282
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096847.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096847.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC TGATGCTCCA AGCTTTCCAT CATCCATCTG TTCCCCTCCT 
GTATATCCTG TGTCTCCAAC AGAAAACAGT CAACAGGATA AAAAGCAAAC TAAAGACTCT 
ATTACCTACA GTGTTGGTCA GATGAATACT CAGGATAAAA AGCAAACTCT ATTAACCTAC 
AGTGTTGGTC AGATAAAGTC TCAGGATATA AAGCAAACTA AAGACACTGT TACATATAGA 
GAAGGCCACA TAAACGCGCA GGATGAACAG CAAATGAAAG ACTCCATTAC ATACAGTGAA 
GCCAAGATAA GTACTCGGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCGTCTT GGATACTTTG GAGCAAGTCT GTATGGGACA 
TCTTCACATC TGCCCTGGCT TGCTGGATTA ATTGAAGCTC CAAGTCTATG TAACACTTGC 
TCCAGAGTCT CCATCCCAAT CTCAGCATTA TGTGCCTGTG CCTCTTCTGG ATCCCATATG 
TCCCATTGGG ATAGCAGTCA TAAGGAGAAC AATCAAACTA AAGACATGAT TACATACAGT 
GAAGTTCAGA CAAGTATGCA GGATGAACAA CAAGCTAAAG GCACTATTAC ATACAGTGAT 
GGTCAGGTAA AAACTCATGT GTTTGCAGAA CAAAAGTCCA ATCACCAAGG CACCAGAAGC 
ATCAGACAAG AAGGTGGTCA GATGTCAGCG CTGATCAAGA ATAGCGAAAT ATCTGAAGAC 
AATATTACAG ATGATATAAG ATCTCTGTTA AGGCCTCATC CTGAGTGTCC AGTTCAGTTT 
TCGGGCCCAG CCCTTATAAA GGATGTTAGT GAGCTGGAGA GAGTGCAGTG CCATTCAACT 
AAACTGGTAA AAGAGATGGG AGAATTTAGC TATGAGGAAA GACAATCAAG TGCAAGCTTT 
CCCCTGCAGC TCCCATTCAA ACAGGGAGTT TCCAAAGACT TGGAGGACAT CACTTCAACC 
TATGTCTATA GCCCTGGGAT TCATACTGCA TGCATAGGCA ATAACAATGT TCTGAAGTCT 
GTGTATACGT ATAAAGGTTT GCAAACTGAC AGCCTTCATG CTGATATCAT GAATGGTAAT 
GAGTGCAAAT GCACAGAAGA GACGGCCAAT CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096848.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952337.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X11, mRNA.

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CloneID	OXa46454
Clone ID Related Accession (Same CDS sequence)	XM_018096848.1
Accession Version	XM_018096848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA AGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGAAGT TCAGATGAAT 
TCTAGGAGAA ATATGCAGGG CAAAGAAGAA TCTGATTATA ATGATTATAC TGTTACATTC 
AGTGAAGGTC AGAGAAATAC ACAGAATAAA AAACAGTCTG ATGATACTAT TATATACAGT 
AAAGTTCAGA TAAATACTCA GGATAAAGAG CATATTAAAG ACTCTATTAC TTACAGTGAA 
GTTCAGATAA ATACTCAGGA TAAAGTGCAT ACTAAAGACA CTATTACATA CAGTGATGTT 
CTGATGAATT CTAGGAGAAA TATGCAGGAT AAAGAGCAAA CTAAAGACAC TATTACATAC 
ACTGAAGCGC AGAGACATTC TCAGAATTTA GAAAATGTTG ATCAGTCTGC ATCTAACAAG 
CAAAATGTGA TGAGCAGTGT ATCTGGGGAT CATCTTGGAT ACTCTGGGTC AGGTCTATAT 
GGCGCCTCTT TACATCAGAC CAGATCTGCA GGATTATCCG AAGCTACAAC TCTATATAAA 
AATGTCTCTG GAGTCTCAAT CTCAGCCTCT GCATCATCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCC CAATGGAAAG CAGTGTTAAG AATTTAGAAA ATGTTGGTCA GTCTGCTTCT 
AATAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG GAGTCTCCAT CTCAGCCTCT GCATCGTCCT CTTCTGGATA CCTTGCATCC 
CCAATGGAAA ACAGTGTTAA GGATAAAGAG CAAACTAAAG ACACTATTAC ATACACTGAA 
GCTCAGAGAA ATTCTCAGGA TAAAGAACAA ACTAAAGAAA CTATTACATA CAGGGAAAGC 
CAGATAAATA CTCAGAATTT AGAAAATGTT GGCCGGTCTG CCTCTAACAA GCAAAATGGG 
ATGAGCAGTG TATCTGGTGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTTT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCA 
GGAGGCTCCA TCTCAGCCTC TGCATCATCC TCTTCTGGAT ACCTTGCATC CTCAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACCAAA GACACTTTTA CATACATTGA AGCTCAGATA 
AATTCTCAGG GCAAAGAGCA AACTAAAGAT GTTATTACAT ACAGTGAAGC TCAGAGAAAT 
TTTCAGGATA AAGAGCAACC TAAAGAGAGT ATTACATGTA GTGAAGTTTA TAGAAGTACT 
CAGGATAAAG AGCAAACTAA AGACACAATT ACATACAGTA AAGTTCAGAG AAATATGCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCATC TAGGAAACAC TGGACCAAGT CTGTATGGGG CTTCTTTAGC AGGATTATCT 
GATGCTCCAA GCTTTCCATC ATCCATCTGT TCCCCTCCTG TATATCCTGT GTCTCCAACA 
GAAAACAGTC AACAGGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCGCAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGTGAAG CCAAGATAAG TACTCGGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACAT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGAAGCA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CCCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACGGCCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952337.1
CDS	452..10261
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096848.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096848.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA AGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGAAGT TCAGATGAAT 
TCTAGGAGAA ATATGCAGGG CAAAGAAGAA TCTGATTATA ATGATTATAC TGTTACATTC 
AGTGAAGGTC AGAGAAATAC ACAGAATAAA AAACAGTCTG ATGATACTAT TATATACAGT 
AAAGTTCAGA TAAATACTCA GGATAAAGAG CATATTAAAG ACTCTATTAC TTACAGTGAA 
GTTCAGATAA ATACTCAGGA TAAAGTGCAT ACTAAAGACA CTATTACATA CAGTGATGTT 
CTGATGAATT CTAGGAGAAA TATGCAGGAT AAAGAGCAAA CTAAAGACAC TATTACATAC 
ACTGAAGCGC AGAGACATTC TCAGAATTTA GAAAATGTTG ATCAGTCTGC ATCTAACAAG 
CAAAATGTGA TGAGCAGTGT ATCTGGGGAT CATCTTGGAT ACTCTGGGTC AGGTCTATAT 
GGCGCCTCTT TACATCAGAC CAGATCTGCA GGATTATCCG AAGCTACAAC TCTATATAAA 
AATGTCTCTG GAGTCTCAAT CTCAGCCTCT GCATCATCTG CATCATCCTC TTCTGGATAC 
CTTGCATCCC CAATGGAAAG CAGTGTTAAG AATTTAGAAA ATGTTGGTCA GTCTGCTTCT 
AATAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG GAGTCTCCAT CTCAGCCTCT GCATCGTCCT CTTCTGGATA CCTTGCATCC 
CCAATGGAAA ACAGTGTTAA GGATAAAGAG CAAACTAAAG ACACTATTAC ATACACTGAA 
GCTCAGAGAA ATTCTCAGGA TAAAGAACAA ACTAAAGAAA CTATTACATA CAGGGAAAGC 
CAGATAAATA CTCAGAATTT AGAAAATGTT GGCCGGTCTG CCTCTAACAA GCAAAATGGG 
ATGAGCAGTG TATCTGGTGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTTT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCA 
GGAGGCTCCA TCTCAGCCTC TGCATCATCC TCTTCTGGAT ACCTTGCATC CTCAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACCAAA GACACTTTTA CATACATTGA AGCTCAGATA 
AATTCTCAGG GCAAAGAGCA AACTAAAGAT GTTATTACAT ACAGTGAAGC TCAGAGAAAT 
TTTCAGGATA AAGAGCAACC TAAAGAGAGT ATTACATGTA GTGAAGTTTA TAGAAGTACT 
CAGGATAAAG AGCAAACTAA AGACACAATT ACATACAGTA AAGTTCAGAG AAATATGCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCATC TAGGAAACAC TGGACCAAGT CTGTATGGGG CTTCTTTAGC AGGATTATCT 
GATGCTCCAA GCTTTCCATC ATCCATCTGT TCCCCTCCTG TATATCCTGT GTCTCCAACA 
GAAAACAGTC AACAGGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCGCAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGTGAAG CCAAGATAAG TACTCGGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACAT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGAAGCA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CCCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACGGCCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096849.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952338.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X12, mRNA.

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CloneID	OXa46455
Clone ID Related Accession (Same CDS sequence)	XM_018096849.1
Accession Version	XM_018096849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGTGCAAG CTTTCCCCTG CAGCTCCCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACGGC CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952338.1
CDS	452..10246
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDISASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096849.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096849.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGTGCAAG CTTTCCCCTG CAGCTCCCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACGGC CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096850.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952339.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X13, mRNA.

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CloneID	OXa46456
Clone ID Related Accession (Same CDS sequence)	XM_018096850.1
Accession Version	XM_018096850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGAAT AAAAAACAGT CTGATGATAC TATTATATAC 
AGTAAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAT 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGATCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAT 
AAAAATGTCT CTGGAGTCTC AATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAATAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGGAGTCTC CATCTCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCCAATGG AAAACAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCTCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG TGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TTTTTACATC AGACCAGATC TGCAGGATTA TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCCTCTTCTG GATACCTTGC ATCCTCAATG 
GAAAGCAGTG TTAAGGATAA AGAGCAAACC AAAGACACTT TTACATACAT TGAAGCTCAG 
ATAAATTCTC AGGGCAAAGA GCAAACTAAA GATGTTATTA CATACAGTGA AGCTCAGAGA 
AATTTTCAGG ATAAAGAGCA ACCTAAAGAG AGTATTACAT GTAGTGAAGT TTATAGAAGT 
ACTCAGGATA AAGAGCAAAC TAAAGACACA ATTACATACA GTAAAGTTCA GAGAAATATG 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTAGGAAA CACTGGACCA AGTCTGTATG GGGCTTCTTT AGCAGGATTA 
TCTGATGCTC CAAGCTTTCC ATCATCCATC TGTTCCCCTC CTGTATATCC TGTGTCTCCA 
ACAGAAAACA GTCAACAGGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCG 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGTG AAGCCAAGAT AAGTACTCGG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CATCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952339.1
CDS	452..10204
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096850.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096850.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGAAT AAAAAACAGT CTGATGATAC TATTATATAC 
AGTAAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAT 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGATCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAT 
AAAAATGTCT CTGGAGTCTC AATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAATAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGGAGTCTC CATCTCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCCAATGG AAAACAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCTCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG TGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TTTTTACATC AGACCAGATC TGCAGGATTA TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCCTCTTCTG GATACCTTGC ATCCTCAATG 
GAAAGCAGTG TTAAGGATAA AGAGCAAACC AAAGACACTT TTACATACAT TGAAGCTCAG 
ATAAATTCTC AGGGCAAAGA GCAAACTAAA GATGTTATTA CATACAGTGA AGCTCAGAGA 
AATTTTCAGG ATAAAGAGCA ACCTAAAGAG AGTATTACAT GTAGTGAAGT TTATAGAAGT 
ACTCAGGATA AAGAGCAAAC TAAAGACACA ATTACATACA GTAAAGTTCA GAGAAATATG 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTAGGAAA CACTGGACCA AGTCTGTATG GGGCTTCTTT AGCAGGATTA 
TCTGATGCTC CAAGCTTTCC ATCATCCATC TGTTCCCCTC CTGTATATCC TGTGTCTCCA 
ACAGAAAACA GTCAACAGGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCG 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGTG AAGCCAAGAT AAGTACTCGG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CATCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096851.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952340.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X14, mRNA.

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CloneID	OXa46457
Clone ID Related Accession (Same CDS sequence)	XM_018096851.1
Accession Version	XM_018096851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAT 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGATCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAT 
AAAAATGTCT CTGGAGTCTC AATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAATAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGGAGTCTC CATCTCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCCAATGG AAAACAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCTCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG TGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TTTTTACATC AGACCAGATC TGCAGGATTA TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCCTCTTCTG GATACCTTGC ATCCTCAATG 
GAAAGCAGTG TTAAGGATAA AGAGCAAACC AAAGACACTT TTACATACAT TGAAGCTCAG 
ATAAATTCTC AGGGCAAAGA GCAAACTAAA GATGTTATTA CATACAGTGA AGCTCAGAGA 
AATTTTCAGG ATAAAGAGCA ACCTAAAGAG AGTATTACAT GTAGTGAAGT TTATAGAAGT 
ACTCAGGATA AAGAGCAAAC TAAAGACACA ATTACATACA GTAAAGTTCA GAGAAATATG 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTAGGAAA CACTGGACCA AGTCTGTATG GGGCTTCTTT AGCAGGATTA 
TCTGATGCTC CAAGCTTTCC ATCATCCATC TGTTCCCCTC CTGTATATCC TGTGTCTCCA 
ACAGAAAACA GTCAACAGGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCG 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGTG AAGCCAAGAT AAGTACTCGG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CATCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952340.1
CDS	452..10204
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096851.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096851.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAT 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGATCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAT 
AAAAATGTCT CTGGAGTCTC AATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAATAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGGAGTCTC CATCTCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCCAATGG AAAACAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCTCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG TGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TTTTTACATC AGACCAGATC TGCAGGATTA TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCCTCTTCTG GATACCTTGC ATCCTCAATG 
GAAAGCAGTG TTAAGGATAA AGAGCAAACC AAAGACACTT TTACATACAT TGAAGCTCAG 
ATAAATTCTC AGGGCAAAGA GCAAACTAAA GATGTTATTA CATACAGTGA AGCTCAGAGA 
AATTTTCAGG ATAAAGAGCA ACCTAAAGAG AGTATTACAT GTAGTGAAGT TTATAGAAGT 
ACTCAGGATA AAGAGCAAAC TAAAGACACA ATTACATACA GTAAAGTTCA GAGAAATATG 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTAGGAAA CACTGGACCA AGTCTGTATG GGGCTTCTTT AGCAGGATTA 
TCTGATGCTC CAAGCTTTCC ATCATCCATC TGTTCCCCTC CTGTATATCC TGTGTCTCCA 
ACAGAAAACA GTCAACAGGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCG 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGTG AAGCCAAGAT AAGTACTCGG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CATCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096852.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952341.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X15, mRNA.

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CloneID	OXa46458
Clone ID Related Accession (Same CDS sequence)	XM_018096852.1
Accession Version	XM_018096852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GACACTATTA CATACAGTGA AGTTCAGATG 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAATCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGAAT AAAAAACAGT CTGATGATAC TATTATATAC 
AGTAAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAT 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGATCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAT 
AAAAATGTCT CTGGAGTCTC AATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAATAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGGAGTCTC CATCTCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCCAATGG AAAACAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCTCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG TGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TTTTTACATC AGACCAGATC TGCAGGATTA TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCCTCTTCTG GATACCTTGC ATCCTCAATG 
GAAAGCAGTG TTAAGGATAA AGAGCAAACC AAAGACACTT TTACATACAT TGAAGCTCAG 
ATAAATTCTC AGGGCAAAGA GCAAACTAAA GATGTTATTA CATACAGTGA AGCTCAGAGA 
AATTTTCAGG ATAAAGAGCA ACCTAAAGAG AGTATTACAT GTAGTGAAGT TTATAGAAGT 
ACTCAGGATA AAGAGCAAAC TAAAGACACA ATTACATACA GTAAAGTTCA GAGAAATATG 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTAGGAAA CACTGGACCA AGTCTGTATG GGGCTTCTTT AGCAGGATTA 
TCTGATGCTC CAAGCTTTCC ATCATCCATC TGTTCCCCTC CTGTATATCC TGTGTCTCCA 
ACAGAAAACA GTCAACAGGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCG 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGTG AAGCCAAGAT AAGTACTCGG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CATCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952341.1
CDS	452..10204
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096852.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096852.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GACACTATTA CATACAGTGA AGTTCAGATG 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAATCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGAAT AAAAAACAGT CTGATGATAC TATTATATAC 
AGTAAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAT 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGATCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAT 
AAAAATGTCT CTGGAGTCTC AATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAATAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGGAGTCTC CATCTCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCCAATGG AAAACAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCTCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG TGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TTTTTACATC AGACCAGATC TGCAGGATTA TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCCTCTTCTG GATACCTTGC ATCCTCAATG 
GAAAGCAGTG TTAAGGATAA AGAGCAAACC AAAGACACTT TTACATACAT TGAAGCTCAG 
ATAAATTCTC AGGGCAAAGA GCAAACTAAA GATGTTATTA CATACAGTGA AGCTCAGAGA 
AATTTTCAGG ATAAAGAGCA ACCTAAAGAG AGTATTACAT GTAGTGAAGT TTATAGAAGT 
ACTCAGGATA AAGAGCAAAC TAAAGACACA ATTACATACA GTAAAGTTCA GAGAAATATG 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTAGGAAA CACTGGACCA AGTCTGTATG GGGCTTCTTT AGCAGGATTA 
TCTGATGCTC CAAGCTTTCC ATCATCCATC TGTTCCCCTC CTGTATATCC TGTGTCTCCA 
ACAGAAAACA GTCAACAGGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCG 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGTG AAGCCAAGAT AAGTACTCGG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CATCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGAA GCATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCCCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACGGCCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096853.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952342.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X16, mRNA.

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CloneID	OXa46459
Clone ID Related Accession (Same CDS sequence)	XM_018096853.1
Accession Version	XM_018096853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCTCAG AGAAATTCTC AGGATAAAGA ACAAACTAAA 
GAAACTATTA CATACAGGGA AAGCCAGATA AATACTCAGA ATTTAGAAAA TGTTGGCCGG 
TCTGCCTCTA ACAAGCAAAA TGGGATGAGC AGTGTATCTG GTGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTTTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCAGGAGGC TCCATCTCAG CCTCTGCATC ATCCTCTTCT 
GGATACCTTG CATCCTCAAT GGAAAGCAGT GTTAAGGATA AAGAGCAAAC CAAAGACACT 
TTTACATACA TTGAAGCTCA GATAAATTCT CAGGGCAAAG AGCAAACTAA AGATGTTATT 
ACATACAGTG AAGCTCAGAG AAATTTTCAG GATAAAGAGC AACCTAAAGA GAGTATTACA 
TGTAGTGAAG TTTATAGAAG TACTCAGGAT AAAGAGCAAA CTAAAGACAC AATTACATAC 
AGTAAAGTTC AGAGAAATAT GCAGAATCTA GAAAATGTTG GTCGGTCTGC CCCTAACAGG 
CAAAATGTGA TGAGCAGTGT ATCTGGGGAT CATCTAGGAA ACACTGGACC AAGTCTGTAT 
GGGGCTTCTT TAGCAGGATT ATCTGATGCT CCAAGCTTTC CATCATCCAT CTGTTCCCCT 
CCTGTATATC CTGTGTCTCC AACAGAAAAC AGTCAACAGG ATAAAAAGCA AACTAAAGAC 
TCTATTACCT ACAGTGTTGG TCAGATGAAT ACTCAGGATA AAAAGCAAAC TCTATTAACC 
TACAGTGTTG GTCAGATAAA GTCTCAGGAT ATAAAGCAAA CTAAAGACAC TGTTACATAT 
AGAGAAGGCC ACATAAACGC GCAGGATGAA CAGCAAATGA AAGACTCCAT TACATACAGT 
GAAGCCAAGA TAAGTACTCG GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCGT CTTGGATACT TTGGAGCAAG TCTGTATGGG 
ACATCTTCAC ATCTGCCCTG GCTTGCTGGA TTAATTGAAG CTCCAAGTCT ATGTAACACT 
TGCTCCAGAG TCTCCATCCC AATCTCAGCA TTATGTGCCT GTGCCTCTTC TGGATCCCAT 
ATGTCCCATT GGGATAGCAG TCATAAGGAG AACAATCAAA CTAAAGACAT GATTACATAC 
AGTGAAGTTC AGACAAGTAT GCAGGATGAA CAACAAGCTA AAGGCACTAT TACATACAGT 
GATGGTCAGG TAAAAACTCA TGTGTTTGCA GAACAAAAGT CCAATCACCA AGGCACCAGA 
AGCATCAGAC AAGAAGGTGG TCAGATGTCA GCGCTGATCA AGAATAGCGA AATATCTGAA 
GACAATATTA CAGATGATAT AAGATCTCTG TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG 
TTTTCGGGCC CAGCCCTTAT AAAGGATGTT AGTGAGCTGG AGAGAGTGCA GTGCCATTCA 
ACTAAACTGG TAAAAGAGAT GGGAGAATTT AGCTATGAGG AAAGACAATC AAGTGCAAGC 
TTTCCCCTGC AGCTCCCATT CAAACAGGGA GTTTCCAAAG ACTTGGAGGA CATCACTTCA 
ACCTATGTCT ATAGCCCTGG GATTCATACT GCATGCATAG GCAATAACAA TGTTCTGAAG 
TCTGTGTATA CGTATAAAGG TTTGCAAACT GACAGCCTTC ATGCTGATAT CATGAATGGT 
AATGAGTGCA AATGCACAGA AGAGACGGCC AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952342.1
CDS	452..10165
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096853.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096853.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCTCAG AGAAATTCTC AGGATAAAGA ACAAACTAAA 
GAAACTATTA CATACAGGGA AAGCCAGATA AATACTCAGA ATTTAGAAAA TGTTGGCCGG 
TCTGCCTCTA ACAAGCAAAA TGGGATGAGC AGTGTATCTG GTGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTTTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCAGGAGGC TCCATCTCAG CCTCTGCATC ATCCTCTTCT 
GGATACCTTG CATCCTCAAT GGAAAGCAGT GTTAAGGATA AAGAGCAAAC CAAAGACACT 
TTTACATACA TTGAAGCTCA GATAAATTCT CAGGGCAAAG AGCAAACTAA AGATGTTATT 
ACATACAGTG AAGCTCAGAG AAATTTTCAG GATAAAGAGC AACCTAAAGA GAGTATTACA 
TGTAGTGAAG TTTATAGAAG TACTCAGGAT AAAGAGCAAA CTAAAGACAC AATTACATAC 
AGTAAAGTTC AGAGAAATAT GCAGAATCTA GAAAATGTTG GTCGGTCTGC CCCTAACAGG 
CAAAATGTGA TGAGCAGTGT ATCTGGGGAT CATCTAGGAA ACACTGGACC AAGTCTGTAT 
GGGGCTTCTT TAGCAGGATT ATCTGATGCT CCAAGCTTTC CATCATCCAT CTGTTCCCCT 
CCTGTATATC CTGTGTCTCC AACAGAAAAC AGTCAACAGG ATAAAAAGCA AACTAAAGAC 
TCTATTACCT ACAGTGTTGG TCAGATGAAT ACTCAGGATA AAAAGCAAAC TCTATTAACC 
TACAGTGTTG GTCAGATAAA GTCTCAGGAT ATAAAGCAAA CTAAAGACAC TGTTACATAT 
AGAGAAGGCC ACATAAACGC GCAGGATGAA CAGCAAATGA AAGACTCCAT TACATACAGT 
GAAGCCAAGA TAAGTACTCG GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCGT CTTGGATACT TTGGAGCAAG TCTGTATGGG 
ACATCTTCAC ATCTGCCCTG GCTTGCTGGA TTAATTGAAG CTCCAAGTCT ATGTAACACT 
TGCTCCAGAG TCTCCATCCC AATCTCAGCA TTATGTGCCT GTGCCTCTTC TGGATCCCAT 
ATGTCCCATT GGGATAGCAG TCATAAGGAG AACAATCAAA CTAAAGACAT GATTACATAC 
AGTGAAGTTC AGACAAGTAT GCAGGATGAA CAACAAGCTA AAGGCACTAT TACATACAGT 
GATGGTCAGG TAAAAACTCA TGTGTTTGCA GAACAAAAGT CCAATCACCA AGGCACCAGA 
AGCATCAGAC AAGAAGGTGG TCAGATGTCA GCGCTGATCA AGAATAGCGA AATATCTGAA 
GACAATATTA CAGATGATAT AAGATCTCTG TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG 
TTTTCGGGCC CAGCCCTTAT AAAGGATGTT AGTGAGCTGG AGAGAGTGCA GTGCCATTCA 
ACTAAACTGG TAAAAGAGAT GGGAGAATTT AGCTATGAGG AAAGACAATC AAGTGCAAGC 
TTTCCCCTGC AGCTCCCATT CAAACAGGGA GTTTCCAAAG ACTTGGAGGA CATCACTTCA 
ACCTATGTCT ATAGCCCTGG GATTCATACT GCATGCATAG GCAATAACAA TGTTCTGAAG 
TCTGTGTATA CGTATAAAGG TTTGCAAACT GACAGCCTTC ATGCTGATAT CATGAATGGT 
AATGAGTGCA AATGCACAGA AGAGACGGCC AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096854.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952343.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X17, mRNA.

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CloneID	OXa46460
Clone ID Related Accession (Same CDS sequence)	XM_018096854.1
Accession Version	XM_018096854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGACACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGATCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TATAAAAATG TCTCTGGAGT CTCAATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAGCAGT GTTAAGAATT TAGAAAATGT TGGTCAGTCT 
GCTTCTAATA AGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGGAGT CTCCATCTCA GCCTCTGCAT CGTCCTCTTC TGGATACCTT 
GCATCCCCAA TGGAAAACAG TGTTAAGGAT AAAGAGCAAA CTAAAGACAC TATTACATAC 
ACTGAAGCTC AGAGAAATTC TCAGGATAAA GAACAAACTA AAGAAACTAT TACATACAGG 
GAAAGCCAGA TAAATACTCA GAATTTAGAA AATGTTGGCC GGTCTGCCTC TAACAAGCAA 
AATGGGATGA GCAGTGTATC TGGTGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTTTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCAGGAG GCTCCATCTC AGCCTCTGCA TCATCCTCTT CTGGATACCT TGCATCCTCA 
ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA ACCAAAGACA CTTTTACATA CATTGAAGCT 
CAGATAAATT CTCAGGGCAA AGAGCAAACT AAAGATGTTA TTACATACAG TGAAGCTCAG 
AGAAATTTTC AGGATAAAGA GCAACCTAAA GAGAGTATTA CATGTAGTGA AGTTTATAGA 
AGTACTCAGG ATAAAGAGCA AACTAAAGAC ACAATTACAT ACAGTAAAGT TCAGAGAAAT 
ATGCAGAATC TAGAAAATGT TGGTCGGTCT GCCCCTAACA GGCAAAATGT GATGAGCAGT 
GTATCTGGGG ATCATCTAGG AAACACTGGA CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA 
TTATCTGATG CTCCAAGCTT TCCATCATCC ATCTGTTCCC CTCCTGTATA TCCTGTGTCT 
CCAACAGAAA ACAGTCAACA GGATAAAAAG CAAACTAAAG ACTCTATTAC CTACAGTGTT 
GGTCAGATGA ATACTCAGGA TAAAAAGCAA ACTCTATTAA CCTACAGTGT TGGTCAGATA 
AAGTCTCAGG ATATAAAGCA AACTAAAGAC ACTGTTACAT ATAGAGAAGG CCACATAAAC 
GCGCAGGATG AACAGCAAAT GAAAGACTCC ATTACATACA GTGAAGCCAA GATAAGTACT 
CGGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC GTCTTGGATA CTTTGGAGCA AGTCTGTATG GGACATCTTC ACATCTGCCC 
TGGCTTGCTG GATTAATTGA AGCTCCAAGT CTATGTAACA CTTGCTCCAG AGTCTCCATC 
CCAATCTCAG CATTATGTGC CTGTGCCTCT TCTGGATCCC ATATGTCCCA TTGGGATAGC 
AGTCATAAGG AGAACAATCA AACTAAAGAC ATGATTACAT ACAGTGAAGT TCAGACAAGT 
ATGCAGGATG AACAACAAGC TAAAGGCACT ATTACATACA GTGATGGTCA GGTAAAAACT 
CATGTGTTTG CAGAACAAAA GTCCAATCAC CAAGGCACCA GAAGCATCAG ACAAGAAGGT 
GGTCAGATGT CAGCGCTGAT CAAGAATAGC GAAATATCTG AAGACAATAT TACAGATGAT 
ATAAGATCTC TGTTAAGGCC TCATCCTGAG TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT 
ATAAAGGATG TTAGTGAGCT GGAGAGAGTG CAGTGCCATT CAACTAAACT GGTAAAAGAG 
ATGGGAGAAT TTAGCTATGA GGAAAGACAA TCAAGTGCAA GCTTTCCCCT GCAGCTCCCA 
TTCAAACAGG GAGTTTCCAA AGACTTGGAG GACATCACTT CAACCTATGT CTATAGCCCT 
GGGATTCATA CTGCATGCAT AGGCAATAAC AATGTTCTGA AGTCTGTGTA TACGTATAAA 
GGTTTGCAAA CTGACAGCCT TCATGCTGAT ATCATGAATG GTAATGAGTG CAAATGCACA 
GAAGAGACGG CCAATCATCT TAATGACGGC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952343.1
CDS	452..10147
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096854.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096854.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGACACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGATCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TATAAAAATG TCTCTGGAGT CTCAATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAGCAGT GTTAAGAATT TAGAAAATGT TGGTCAGTCT 
GCTTCTAATA AGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGGAGT CTCCATCTCA GCCTCTGCAT CGTCCTCTTC TGGATACCTT 
GCATCCCCAA TGGAAAACAG TGTTAAGGAT AAAGAGCAAA CTAAAGACAC TATTACATAC 
ACTGAAGCTC AGAGAAATTC TCAGGATAAA GAACAAACTA AAGAAACTAT TACATACAGG 
GAAAGCCAGA TAAATACTCA GAATTTAGAA AATGTTGGCC GGTCTGCCTC TAACAAGCAA 
AATGGGATGA GCAGTGTATC TGGTGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTTTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCAGGAG GCTCCATCTC AGCCTCTGCA TCATCCTCTT CTGGATACCT TGCATCCTCA 
ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA ACCAAAGACA CTTTTACATA CATTGAAGCT 
CAGATAAATT CTCAGGGCAA AGAGCAAACT AAAGATGTTA TTACATACAG TGAAGCTCAG 
AGAAATTTTC AGGATAAAGA GCAACCTAAA GAGAGTATTA CATGTAGTGA AGTTTATAGA 
AGTACTCAGG ATAAAGAGCA AACTAAAGAC ACAATTACAT ACAGTAAAGT TCAGAGAAAT 
ATGCAGAATC TAGAAAATGT TGGTCGGTCT GCCCCTAACA GGCAAAATGT GATGAGCAGT 
GTATCTGGGG ATCATCTAGG AAACACTGGA CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA 
TTATCTGATG CTCCAAGCTT TCCATCATCC ATCTGTTCCC CTCCTGTATA TCCTGTGTCT 
CCAACAGAAA ACAGTCAACA GGATAAAAAG CAAACTAAAG ACTCTATTAC CTACAGTGTT 
GGTCAGATGA ATACTCAGGA TAAAAAGCAA ACTCTATTAA CCTACAGTGT TGGTCAGATA 
AAGTCTCAGG ATATAAAGCA AACTAAAGAC ACTGTTACAT ATAGAGAAGG CCACATAAAC 
GCGCAGGATG AACAGCAAAT GAAAGACTCC ATTACATACA GTGAAGCCAA GATAAGTACT 
CGGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC GTCTTGGATA CTTTGGAGCA AGTCTGTATG GGACATCTTC ACATCTGCCC 
TGGCTTGCTG GATTAATTGA AGCTCCAAGT CTATGTAACA CTTGCTCCAG AGTCTCCATC 
CCAATCTCAG CATTATGTGC CTGTGCCTCT TCTGGATCCC ATATGTCCCA TTGGGATAGC 
AGTCATAAGG AGAACAATCA AACTAAAGAC ATGATTACAT ACAGTGAAGT TCAGACAAGT 
ATGCAGGATG AACAACAAGC TAAAGGCACT ATTACATACA GTGATGGTCA GGTAAAAACT 
CATGTGTTTG CAGAACAAAA GTCCAATCAC CAAGGCACCA GAAGCATCAG ACAAGAAGGT 
GGTCAGATGT CAGCGCTGAT CAAGAATAGC GAAATATCTG AAGACAATAT TACAGATGAT 
ATAAGATCTC TGTTAAGGCC TCATCCTGAG TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT 
ATAAAGGATG TTAGTGAGCT GGAGAGAGTG CAGTGCCATT CAACTAAACT GGTAAAAGAG 
ATGGGAGAAT TTAGCTATGA GGAAAGACAA TCAAGTGCAA GCTTTCCCCT GCAGCTCCCA 
TTCAAACAGG GAGTTTCCAA AGACTTGGAG GACATCACTT CAACCTATGT CTATAGCCCT 
GGGATTCATA CTGCATGCAT AGGCAATAAC AATGTTCTGA AGTCTGTGTA TACGTATAAA 
GGTTTGCAAA CTGACAGCCT TCATGCTGAT ATCATGAATG GTAATGAGTG CAAATGCACA 
GAAGAGACGG CCAATCATCT TAATGACGGC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096855.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952344.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X18, mRNA.

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CloneID	OXa46461
Clone ID Related Accession (Same CDS sequence)	XM_018096855.1
Accession Version	XM_018096855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGG ATAAAGAACA AACTAAAGAA 
ACTATTACAT ACAGGGAAAG CCAGATAAAT ACTCAGAATT TAGAAAATGT TGGCCGGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GTATCTGGTG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTT TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC AGGAGGCTCC ATCTCAGCCT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCTCAATGGA AAGCAGTGTT AAGGATAAAG AGCAAACCAA AGACACTTTT 
ACATACATTG AAGCTCAGAT AAATTCTCAG GGCAAAGAGC AAACTAAAGA TGTTATTACA 
TACAGTGAAG CTCAGAGAAA TTTTCAGGAT AAAGAGCAAC CTAAAGAGAG TATTACATGT 
AGTGAAGTTT ATAGAAGTAC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC TGATGCTCCA AGCTTTCCAT CATCCATCTG TTCCCCTCCT 
GTATATCCTG TGTCTCCAAC AGAAAACAGT CAACAGGATA AAAAGCAAAC TAAAGACTCT 
ATTACCTACA GTGTTGGTCA GATGAATACT CAGGATAAAA AGCAAACTCT ATTAACCTAC 
AGTGTTGGTC AGATAAAGTC TCAGGATATA AAGCAAACTA AAGACACTGT TACATATAGA 
GAAGGCCACA TAAACGCGCA GGATGAACAG CAAATGAAAG ACTCCATTAC ATACAGTGAA 
GCCAAGATAA GTACTCGGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCGTCTT GGATACTTTG GAGCAAGTCT GTATGGGACA 
TCTTCACATC TGCCCTGGCT TGCTGGATTA ATTGAAGCTC CAAGTCTATG TAACACTTGC 
TCCAGAGTCT CCATCCCAAT CTCAGCATTA TGTGCCTGTG CCTCTTCTGG ATCCCATATG 
TCCCATTGGG ATAGCAGTCA TAAGGAGAAC AATCAAACTA AAGACATGAT TACATACAGT 
GAAGTTCAGA CAAGTATGCA GGATGAACAA CAAGCTAAAG GCACTATTAC ATACAGTGAT 
GGTCAGGTAA AAACTCATGT GTTTGCAGAA CAAAAGTCCA ATCACCAAGG CACCAGAAGC 
ATCAGACAAG AAGGTGGTCA GATGTCAGCG CTGATCAAGA ATAGCGAAAT ATCTGAAGAC 
AATATTACAG ATGATATAAG ATCTCTGTTA AGGCCTCATC CTGAGTGTCC AGTTCAGTTT 
TCGGGCCCAG CCCTTATAAA GGATGTTAGT GAGCTGGAGA GAGTGCAGTG CCATTCAACT 
AAACTGGTAA AAGAGATGGG AGAATTTAGC TATGAGGAAA GACAATCAAG TGCAAGCTTT 
CCCCTGCAGC TCCCATTCAA ACAGGGAGTT TCCAAAGACT TGGAGGACAT CACTTCAACC 
TATGTCTATA GCCCTGGGAT TCATACTGCA TGCATAGGCA ATAACAATGT TCTGAAGTCT 
GTGTATACGT ATAAAGGTTT GCAAACTGAC AGCCTTCATG CTGATATCAT GAATGGTAAT 
GAGTGCAAAT GCACAGAAGA GACGGCCAAT CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952344.1
CDS	452..9862
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELRPEIKTKLVPRIQIDLQPKIQTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTELQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPKIQTEIQPRIQTEIQPKIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096855.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096855.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA CAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACGACC AGAGATAAAA 
ACTAAGCTAG TACCAAGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAACTACAA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGCTAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGAT ACAACCAAAG ATAAAAACAG AGATACAACC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGGCCAG GAATACAAAC AGAGATACAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGATACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGA ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGCTTC GACCAGAGAT AAAAACTAAG CTAGAACCAG GGATACAAAC AGAGCTACAA 
CCAAAGATAC AAACAGAGCT ACAACCAAAG ATACAAACAG AGATACAACC AAAGATACAA 
ACGGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACTACCGG AGTTACGAAC AACGATAAAA 
ACAGACACAA AATCAGAAAT ACAAACAGAC ATAAAGATAC AAACTGAGGA AAAAATAAAA 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGG ATAAAGAACA AACTAAAGAA 
ACTATTACAT ACAGGGAAAG CCAGATAAAT ACTCAGAATT TAGAAAATGT TGGCCGGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GTATCTGGTG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTT TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC AGGAGGCTCC ATCTCAGCCT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCTCAATGGA AAGCAGTGTT AAGGATAAAG AGCAAACCAA AGACACTTTT 
ACATACATTG AAGCTCAGAT AAATTCTCAG GGCAAAGAGC AAACTAAAGA TGTTATTACA 
TACAGTGAAG CTCAGAGAAA TTTTCAGGAT AAAGAGCAAC CTAAAGAGAG TATTACATGT 
AGTGAAGTTT ATAGAAGTAC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC TGATGCTCCA AGCTTTCCAT CATCCATCTG TTCCCCTCCT 
GTATATCCTG TGTCTCCAAC AGAAAACAGT CAACAGGATA AAAAGCAAAC TAAAGACTCT 
ATTACCTACA GTGTTGGTCA GATGAATACT CAGGATAAAA AGCAAACTCT ATTAACCTAC 
AGTGTTGGTC AGATAAAGTC TCAGGATATA AAGCAAACTA AAGACACTGT TACATATAGA 
GAAGGCCACA TAAACGCGCA GGATGAACAG CAAATGAAAG ACTCCATTAC ATACAGTGAA 
GCCAAGATAA GTACTCGGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCGTCTT GGATACTTTG GAGCAAGTCT GTATGGGACA 
TCTTCACATC TGCCCTGGCT TGCTGGATTA ATTGAAGCTC CAAGTCTATG TAACACTTGC 
TCCAGAGTCT CCATCCCAAT CTCAGCATTA TGTGCCTGTG CCTCTTCTGG ATCCCATATG 
TCCCATTGGG ATAGCAGTCA TAAGGAGAAC AATCAAACTA AAGACATGAT TACATACAGT 
GAAGTTCAGA CAAGTATGCA GGATGAACAA CAAGCTAAAG GCACTATTAC ATACAGTGAT 
GGTCAGGTAA AAACTCATGT GTTTGCAGAA CAAAAGTCCA ATCACCAAGG CACCAGAAGC 
ATCAGACAAG AAGGTGGTCA GATGTCAGCG CTGATCAAGA ATAGCGAAAT ATCTGAAGAC 
AATATTACAG ATGATATAAG ATCTCTGTTA AGGCCTCATC CTGAGTGTCC AGTTCAGTTT 
TCGGGCCCAG CCCTTATAAA GGATGTTAGT GAGCTGGAGA GAGTGCAGTG CCATTCAACT 
AAACTGGTAA AAGAGATGGG AGAATTTAGC TATGAGGAAA GACAATCAAG TGCAAGCTTT 
CCCCTGCAGC TCCCATTCAA ACAGGGAGTT TCCAAAGACT TGGAGGACAT CACTTCAACC 
TATGTCTATA GCCCTGGGAT TCATACTGCA TGCATAGGCA ATAACAATGT TCTGAAGTCT 
GTGTATACGT ATAAAGGTTT GCAAACTGAC AGCCTTCATG CTGATATCAT GAATGGTAAT 
GAGTGCAAAT GCACAGAAGA GACGGCCAAT CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096856.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952345.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X19, mRNA.

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CloneID	OXa46462
Clone ID Related Accession (Same CDS sequence)	XM_018096856.1
Accession Version	XM_018096856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGATCTCT GTTAAGGCCT CATCCTGAGT GTCCAGTTCA GTTTTCGGGC 
CCAGCCCTTA TAAAGGATGT TAGTGAGCTG GAGAGAGTGC AGTGCCATTC AACTAAACTG 
GTAAAAGAGA TGGGAGAATT TAGCTATGAG GAAAGACAAT CAAGTGCAAG CTTTCCCCTG 
CAGCTCCCAT TCAAACAGGG AGTTTCCAAA GACTTGGAGG ACATCACTTC AACCTATGTC 
TATAGCCCTG GGATTCATAC TGCATGCATA GGCAATAACA ATGTTCTGAA GTCTGTGTAT 
ACGTATAAAG GTTTGCAAAC TGACAGCCTT CATGCTGATA TCATGAATGG TAATGAGTGC 
AAATGCACAG AAGAGACGGC CAATCATCTT AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952345.1
CDS	452..8536
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHVNGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITLSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRQTGIQTEIQPGIQRKIQPEIQTKIQKEIRPDIKAEIQPKIQTDTKLGTQTEIQPGTEIQPEIQPEIQIELRPRIQTEIQPDIKAEIQPGIQTEIQPDIKAEIKPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELRPEIKTELPPEIKTELRPEIKTELRPEIKTEIRPEMKTELRPEIKTEIQPGIRTDIQPGIQTDLRTEIQTDLRTEIQKKIQTEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRISWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGREDIILPVTGLVGGTVTLPTYLTLPAAIERISWMFHDKGKKIHLAELRNHKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096856.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096856.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT TCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATTCTCCTG 
GCTGAATACA GAGACCAGAA ATTTATCAGA GTGAATAACA ACTACTTCAT CAACCGGCTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAACC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG AAATTATTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCG GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGTC 
AATGGGAAGC GAATACACCT GGCTGAATTC AGACACCAGA AGTTTATCAC AGTTAATAAG 
AAATATTTCT TAAACCGCCT TGAAAAACCT AATGATGGAA CCGCATTAAG GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTTAATGG ACGAAAAAGA 
GACATTTCTT ACATTCTAGC CGTTTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTTTATCGCT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAATGCATCA GTCCCTTTAA AGTCACTTCA TGGGAGACAA 
ACAGGAATAC AAACTGAGAT ACAACCAGGG ATACAAAGAA AGATACAACC AGAGATACAA 
ACAAAGATAC AAAAAGAGAT ACGACCAGAC ATAAAAGCAG AGATTCAACC AAAAATACAA 
ACAGACACAA AACTAGGAAC GCAGACAGAG ATACAACCTG GAACAGAGAT ACAACCAGAA 
ATACAACCAG AGATACAAAT AGAGCTACGA CCCAGGATAC AAACAGAGAT ACAACCAGAT 
ATAAAAGCAG AGATACAACC AGGGATACAA ACAGAGATAC AACCAGATAT AAAAGCAGAG 
ATAAAACCAA AGATACAAGC AGGGATACAC ACTGAGCTAC AACCAAAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAAAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAAATAA AAACAGAGTT ACGACCAGAG 
ATAAAAACAG AGCTTCCACC AGAAATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTTCGACCAG AGATAAAAAC AGAGATACGA CCAGAGATGA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AAATACAACC AGGGATACGT ACAGATATAC AACCAGGGAT ACAAACAGAT 
CTACGAACAG AGATACAAAC AGATCTACGA ACAGAGATAC AAAAAAAGAT ACAAACAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATTTCATG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTAGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGG GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGGGAAG ACATCATTCT GCCGGTGACT GGCCTTGTAG GGGGGACTGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT GGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGAATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCTGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATGGTGGGAG ACAAACAGGA 
ATAAAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAATGGAGAC ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAT AAAAACAGAG 
CTACAACCAG AGATAAAAAC AGAAATGCAG CCAGGGATAA AAACAGAGAA ACAACCAGGG 
ATACTAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGATAAAA ACAGAGATGC GACCAGGGGT GCAAAAAGAG 
ATACAACTAG GAATACAAAA AGAAATACGA TCAGAGATAC AAACTGAGAT ACGACAAGGG 
ATACAGAGAG AGACACAAAG AGAGACAGAA ATAAAAGCAG GAGGAGACAT CCAGGTGATT 
GGCCGTGTAG GGGAGACTGT CACACTGCAG TGCCCTCTGA ATCTACCAAC AAGAATCTTG 
TGGACGTATG ATAATAATGG GGAAAAAATA CTCCTGGCTG AATACAGAGA CCAGAAGTTT 
ATCAGAGTGA ATAACAACTA CTTCATCAAC CGGCTGGAAG GATCTAACAG TGGCACCGCA 
TTAAGGATCA AAAACCTGAG AATGGAGGAT AGTGGGGTCT TCACTGCTCA CATCACTGTT 
AATGGAAGCG CTACAGAAAT TAGTTATATT GTCAATGTAT TTGGAAGAGG AGACATCATT 
GTCCAGGCAA CTGGCCGTGA AGGGGACACT GTCACACTGC CAACCTATAT GAATCACGCA 
ACTTCCATTG AAAGAATCTC ATGGATGTTT CATGACAAAG GGAAGAAAAT ACACCTGGCT 
GAATTCAGAA ACCACAAGTT TATCAGAGTA AATAAGAATT ATTTCCCCAA CCGTCTTGAA 
AAATCGAATT ATGGAACCGC ATTGCAGATC AAAGAGCTGA GAATGGAGGA TTCTGGGATC 
TTCACTGCAC ACATTTATGC TAATGGAAGA AAGAGAGACA TTTCTTATAT TCTTGTTGTG 
TTCGAGCTTA TACCAACACC AGTAATAGAA GTGACGTATG ATGAGAATTT TACTGATCTC 
TGCCATCTTC ATTGTTCTGT CAATTCAAAC TCAACAACCC TGTCCTACAG TTGGACCTAC 
AGAGACAATG GCACTGACAT ACTATATGCC AATGGCAGCA CAATCAGTAT ATTACTAAAG 
AATATGGCAT CAGGCTCAGA ATTTACCTGC TTGGTACAAA ATCCTGTTCA CAGGAACAGT 
GCGTCAGTCC TTTTAAAGTC ACTTGGTGGA AGACGAACAG ATATACGAAA AGAGATACAA 
ACTGAGATAC GACAAGGAAT ACAAAGAGAG ATACGGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGAAC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAA 
AGAGAGATAC AGCCGGAGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCATGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GAATACAAAA AGAGACACGA 
AGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACTTACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GAGACTATTG CATACAGTGA ATTTCAGGGA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTGTTACA 
TTCAGTGAAG GTCAGAGAAA TACACAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GAAGTTCAGA TGAATTCTAG GAGAAATATG CAGGGCAAAG AAGAATCTGA TTATAATGAT 
TATACTGTTA CATTCAGTGA AGGTCAGAGA AATACACAGA ATAAAAAACA GTCTGATGAT 
ACTATTATAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGAAGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ATGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGATCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ATAAAAATGT CTCTGGAGTC TCAATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGC ATCCCCAATG GAAAGCAGTG TTAAGAATTT AGAAAATGTT 
GGTCAGTCTG CTTCTAATAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGGAGTC TCCATCTCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCCAAT GGAAAACAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCTCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGTGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTTTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCCTCTTC TGGATACCTT 
GCATCCTCAA TGGAAAGCAG TGTTAAGGAT AAAGAGCAAA CCAAAGACAC TTTTACATAC 
ATTGAAGCTC AGATAAATTC TCAGGGCAAA GAGCAAACTA AAGATGTTAT TACATACAGT 
GAAGCTCAGA GAAATTTTCA GGATAAAGAG CAACCTAAAG AGAGTATTAC ATGTAGTGAA 
GTTTATAGAA GTACTCAGGA TAAAGAGCAA ACTAAAGACA CAATTACATA CAGTAAAGTT 
CAGAGAAATA TGCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTAGGA AACACTGGAC CAAGTCTGTA TGGGGCTTCT 
TTAGCAGGAT TATCTGATGC TCCAAGCTTT CCATCATCCA TCTGTTCCCC TCCTGTATAT 
CCTGTGTCTC CAACAGAAAA CAGTCAACAG GATAAAAAGC AAACTAAAGA CTCTATTACC 
TACAGTGTTG GTCAGATGAA TACTCAGGAT AAAAAGCAAA CTCTATTAAC CTACAGTGTT 
GGTCAGATAA AGTCTCAGGA TATAAAGCAA ACTAAAGACA CTGTTACATA TAGAGAAGGC 
CACATAAACG CGCAGGATGA ACAGCAAATG AAAGACTCCA TTACATACAG TGAAGCCAAG 
ATAAGTACTC GGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCG TCTTGGATAC TTTGGAGCAA GTCTGTATGG GACATCTTCA 
CATCTGCCCT GGCTTGCTGG ATTAATTGAA GCTCCAAGTC TATGTAACAC TTGCTCCAGA 
GTCTCCATCC CAATCTCAGC ATTATGTGCC TGTGCCTCTT CTGGATCCCA TATGTCCCAT 
TGGGATAGCA GTCATAAGGA GAACAATCAA ACTAAAGACA TGATTACATA CAGTGAAGTT 
CAGACAAGTA TGCAGGATGA ACAACAAGCT AAAGGCACTA TTACATACAG TGATGGTCAG 
GTAAAAACTC ATGTGTTTGC AGAACAAAAG TCCAATCACC AAGGCACCAG AAGCATCAGA 
CAAGAAGGTG GTCAGATGTC AGCGCTGATC AAGAATAGCG AAATATCTGA AGACAATATT 
ACAGATGATA TAAGATCTCT GTTAAGGCCT CATCCTGAGT GTCCAGTTCA GTTTTCGGGC 
CCAGCCCTTA TAAAGGATGT TAGTGAGCTG GAGAGAGTGC AGTGCCATTC AACTAAACTG 
GTAAAAGAGA TGGGAGAATT TAGCTATGAG GAAAGACAAT CAAGTGCAAG CTTTCCCCTG 
CAGCTCCCAT TCAAACAGGG AGTTTCCAAA GACTTGGAGG ACATCACTTC AACCTATGTC 
TATAGCCCTG GGATTCATAC TGCATGCATA GGCAATAACA ATGTTCTGAA GTCTGTGTAT 
ACGTATAAAG GTTTGCAAAC TGACAGCCTT CATGCTGATA TCATGAATGG TAATGAGTGC 
AAATGCACAG AAGAGACGGC CAATCATCTT AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_018096857.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952346.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X20, mRNA.

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CloneID	OXa46463
Clone ID Related Accession (Same CDS sequence)	XM_018096857.1
Accession Version	XM_018096857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTAAT AGAAGTGACA 
TCTGATGAGA ATTTCCCTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAGTGTGTCA GTCGTTTTAA AGTCATATGG TGGGAGACAA 
ACAGGAATAA AACCAAAGAT ACGACCAGAG ATACAACAAA AAATACAAAT GGAGACACGA 
CCAGAGATAA AAACAGAGAT ACGACCAGGG ATAAAAACAG AGATACGACC AGGGATAAAA 
ACAGAGCTAC AACCAGAGAT AAAAACAGAA ATGCAGCCAG GGATAAAAAC AGAGAAACAA 
CCAGGGATAC TAACAGAGAT ACGACCAGGG ATAAAAACAG AGATACAACC AGAGATAAAA 
ACAGAAATAC AGCCAGGGAT AAAAACAGAG AAACAACCAG GGATACTAAC GGAGATACGA 
CCAGGGATAA AAACAGAGAT GCGACCAGGG ATAAAAACAG AGATGCGACC AGGGGTGCAA 
AAAGAGATAC AACTAGGAAT ACAAAAAGAA ATACGATCAG AGATACAAAC TGAGATACGA 
CAAGGGATAC AGAGAGAGAC ACAAAGAGAG ACAGAAATAA AAGCAGGAGG AGACATCCAG 
GTGATTGGCC GTGTAGGGGA GACTGTCACA CTGCAGTGCC CTCTGAATCT ACCAACAAGA 
ATCTTGTGGA CGTATGATAA TAATGGGGAA AAAATACTCC TGGCTGAATA CAGAGACCAG 
AAGTTTATCA GAGTGAATAA CAACTACTTC ATCAACCGGC TGGAAGGATC TAACAGTGGC 
ACCGCATTAA GGATCAAAAA CCTGAGAATG GAGGATAGTG GGGTCTTCAC TGCTCACATC 
ACTGTTAATG GAAGCGCTAC AGAAATTAGT TATATTGTCA ATGTATTTGG AAGAGGAGAC 
ATCATTGTCC AGGCAACTGG CCGTGAAGGG GACACTGTCA CACTGCCAAC CTATATGAAT 
CACGCAACTT CCATTGAAAG AATCTCATGG ATGTTTCATG ACAAAGGGAA GAAAATACAC 
CTGGCTGAAT TCAGAAACCA CAAGTTTATC AGAGTAAATA AGAATTATTT CCCCAACCGT 
CTTGAAAAAT CGAATTATGG AACCGCATTG CAGATCAAAG AGCTGAGAAT GGAGGATTCT 
GGGATCTTCA CTGCACACAT TTATGCTAAT GGAAGAAAGA GAGACATTTC TTATATTCTT 
GTTGTGTTCG AGCTTATACC AACACCAGTA ATAGAAGTGA CGTATGATGA GAATTTTACT 
GATCTCTGCC ATCTTCATTG TTCTGTCAAT TCAAACTCAA CAACCCTGTC CTACAGTTGG 
ACCTACAGAG ACAATGGCAC TGACATACTA TATGCCAATG GCAGCACAAT CAGTATATTA 
CTAAAGAATA TGGCATCAGG CTCAGAATTT ACCTGCTTGG TACAAAATCC TGTTCACAGG 
AACAGTGCGT CAGTCCTTTT AAAGTCACTT GGTGGAAGAC GAACAGATAT ACGAAAAGAG 
ATACAAACTG AGATACGACA AGGAATACAA AGAGAGATAC GGCCGGAGAT ACAAACTGAG 
ATACGACCAG GGATACAAAG AGAGATACAG CCGGAGATAC AAACTGAGAT ACGAACAGGG 
ATACAAAGAG AGATACAGCC GGAGATACAA ACTGAGATAC GACCAGGGAT ACAAAGAGAG 
ATACAGCCGG AGATACAAAC TGAGATACGA ACAGGGATAC AAAGAGAGAT ACAGCCGGAG 
ATACAAACTG AGATACGACC AGGGATACAA AGAGAGATAC AGCCGGAGAT ACAAACTGAG 
ATACGACCAG GGATACAAAG AGAGATACAG CCGGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAAAGAG AGATACAGCC GGAGATACAA AGAGAGATAC AGCCGGAGAT ACAAACTGAG 
ATACGACCAT GGATACAAAA AGAGATACAG CCGGAGATAC AAACAGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GGAGATACAA ACTGAGATAC GACCAGGAAT ACAAAAAGAG 
ACACGAAGAA AGATACATAA AGAGATAGAA CCAAATATAC AAACAGAGAT AGAAAAAGAA 
ATGCAACCAG ACATACGACC AGAAATACAT ACAGAGATAG AAAAAGATAT TCAACCAGAG 
ATACAATCAG AAACACCGAG AGAGGCACAA ATAAAAGGAC CGAAAAAAGA TTCTCGTATA 
CTGACAGTAA TAATTGCAGT TTCGATACTG TGCGGAATAT TTGTTGGTAT AGCTCTTTAT 
TATGGAATAA GACGACGACT AAAGGTTAAA AATCAGTCTA ATTGCACTAT TACATACAGT 
GAAGTTCTGA CAAGTACTCA GGATAAAGAG CATATTAAAG ACTCTATTAC TTACAGTGAA 
GTTCAGATAA ATACTCAGGA TAAAGTGCAT ACTAAAGAGA CTATTGCATA CAGTGAATTT 
CAGGGAAATT CTAGGAGAAA TATGCAGGGC AAAGAAGAAC CTGATTATAA TGATTATACT 
GTTACATTCA GTGAAGGTCA GAGAAATACA CAGGATAAAG AGCATATTAA AGACTCTATT 
ACTTACAGTG AAGTTCAGAT AAATACTCAG GATAAAGTGC ATACTAAAGA CACTATTACA 
TACAGTGAAG TTCAGATGAA TTCTAGGAGA AATATGCAGG GCAAAGAAGA ATCTGATTAT 
AATGATTATA CTGTTACATT CAGTGAAGGT CAGAGAAATA CACAGAATAA AAAACAGTCT 
GATGATACTA TTATATACAG TAAAGTTCAG ATAAATACTC AGGATAAAGA GCATATTAAA 
GACTCTATTA CTTACAGTGA AGTTCAGATA AATACTCAGG ATAAAGTGCA TACTAAAGAC 
ACTATTACAT ACAGTGATGT TCTGATGAAT TCTAGGAGAA ATATGCAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGACATT CTCAGAATTT AGAAAATGTT 
GATCAGTCTG CATCTAACAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATATAA AAATGTCTCT GGAGTCTCAA TCTCAGCCTC TGCATCATCT 
GCATCATCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAATAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GGAGTCTCCA TCTCAGCCTC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AACAGTGTTA AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCTCAGAGA AATTCTCAGG ATAAAGAACA AACTAAAGAA 
ACTATTACAT ACAGGGAAAG CCAGATAAAT ACTCAGAATT TAGAAAATGT TGGCCGGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GTATCTGGTG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTT TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC AGGAGGCTCC ATCTCAGCCT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCTCAATGGA AAGCAGTGTT AAGGATAAAG AGCAAACCAA AGACACTTTT 
ACATACATTG AAGCTCAGAT AAATTCTCAG GGCAAAGAGC AAACTAAAGA TGTTATTACA 
TACAGTGAAG CTCAGAGAAA TTTTCAGGAT AAAGAGCAAC CTAAAGAGAG TATTACATGT 
AGTGAAGTTT ATAGAAGTAC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC TGATGCTCCA AGCTTTCCAT CATCCATCTG TTCCCCTCCT 
GTATATCCTG TGTCTCCAAC AGAAAACAGT CAACAGGATA AAAAGCAAAC TAAAGACTCT 
ATTACCTACA GTGTTGGTCA GATGAATACT CAGGATAAAA AGCAAACTCT ATTAACCTAC 
AGTGTTGGTC AGATAAAGTC TCAGGATATA AAGCAAACTA AAGACACTGT TACATATAGA 
GAAGGCCACA TAAACGCGCA GGATGAACAG CAAATGAAAG ACTCCATTAC ATACAGTGAA 
GCCAAGATAA GTACTCGGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCGTCTT GGATACTTTG GAGCAAGTCT GTATGGGACA 
TCTTCACATC TGCCCTGGCT TGCTGGATTA ATTGAAGCTC CAAGTCTATG TAACACTTGC 
TCCAGAGTCT CCATCCCAAT CTCAGCATTA TGTGCCTGTG CCTCTTCTGG ATCCCATATG 
TCCCATTGGG ATAGCAGTCA TAAGGAGAAC AATCAAACTA AAGACATGAT TACATACAGT 
GAAGTTCAGA CAAGTATGCA GGATGAACAA CAAGCTAAAG GCACTATTAC ATACAGTGAT 
GGTCAGGTAA AAACTCATGT GTTTGCAGAA CAAAAGTCCA ATCACCAAGG CACCAGAAGC 
ATCAGACAAG AAGGTGGTCA GATGTCAGCG CTGATCAAGA ATAGCGAAAT ATCTGAAGAC 
AATATTACAG ATGATATAAG ATCTCTGTTA AGGCCTCATC CTGAGTGTCC AGTTCAGTTT 
TCGGGCCCAG CCCTTATAAA GGATGTTAGT GAGCTGGAGA GAGTGCAGTG CCATTCAACT 
AAACTGGTAA AAGAGATGGG AGAATTTAGC TATGAGGAAA GACAATCAAG TGCAAGCTTT 
CCCCTGCAGC TCCCATTCAA ACAGGGAGTT TCCAAAGACT TGGAGGACAT CACTTCAACC 
TATGTCTATA GCCCTGGGAT TCATACTGCA TGCATAGGCA ATAACAATGT TCTGAAGTCT 
GTGTATACGT ATAAAGGTTT GCAAACTGAC AGCCTTCATG CTGATATCAT GAATGGTAAT 
GAGTGCAAAT GCACAGAAGA GACGGCCAAT CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952346.1
CDS	450..6800
Translation	MKKFTCDILFLLWTSFLITCAEGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRMNGNYFSRRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATDITYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNHKFSRVNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYGGRQTGIKPKIRPEIQQKIQMETRPEIKTEIRPGIKTEIRPGIKTELQPEIKTEMQPGIKTEKQPGILTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQRETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDSGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIRPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQREIQPEIQTEIRPWIQKEIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKETIAYSEFQGNSRRNMQGKEEPDYNDYTVTFSEGQRNTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSEVQMNSRRNMQGKEESDYNDYTVTFSEGQRNTQNKKQSDDTIIYSKVQINTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNGVSISASASSSSGYLASPMENSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGAFLHQTRSAGLSEATTLYKNVSGGSISASASSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDVITYSEAQRNFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSDAPSFPSSICSPPVYPVSPTENSQQDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSEAKISTRNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLPFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETANHLNDGK

Target ORF information:

RefSeq Version	XM_018096857.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096857.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGT 
GCAGAAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA ATGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAAAATC TGAGAATGGA GGATGGTGGG 
GTCTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTACTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
CTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCATA AGTTTAGCAG AGTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTAAT AGAAGTGACA 
TCTGATGAGA ATTTCCCTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CAGAATCCTG CTCACAGGAA CAGTGTGTCA GTCGTTTTAA AGTCATATGG TGGGAGACAA 
ACAGGAATAA AACCAAAGAT ACGACCAGAG ATACAACAAA AAATACAAAT GGAGACACGA 
CCAGAGATAA AAACAGAGAT ACGACCAGGG ATAAAAACAG AGATACGACC AGGGATAAAA 
ACAGAGCTAC AACCAGAGAT AAAAACAGAA ATGCAGCCAG GGATAAAAAC AGAGAAACAA 
CCAGGGATAC TAACAGAGAT ACGACCAGGG ATAAAAACAG AGATACAACC AGAGATAAAA 
ACAGAAATAC AGCCAGGGAT AAAAACAGAG AAACAACCAG GGATACTAAC GGAGATACGA 
CCAGGGATAA AAACAGAGAT GCGACCAGGG ATAAAAACAG AGATGCGACC AGGGGTGCAA 
AAAGAGATAC AACTAGGAAT ACAAAAAGAA ATACGATCAG AGATACAAAC TGAGATACGA 
CAAGGGATAC AGAGAGAGAC ACAAAGAGAG ACAGAAATAA AAGCAGGAGG AGACATCCAG 
GTGATTGGCC GTGTAGGGGA GACTGTCACA CTGCAGTGCC CTCTGAATCT ACCAACAAGA 
ATCTTGTGGA CGTATGATAA TAATGGGGAA AAAATACTCC TGGCTGAATA CAGAGACCAG 
AAGTTTATCA GAGTGAATAA CAACTACTTC ATCAACCGGC TGGAAGGATC TAACAGTGGC 
ACCGCATTAA GGATCAAAAA CCTGAGAATG GAGGATAGTG GGGTCTTCAC TGCTCACATC 
ACTGTTAATG GAAGCGCTAC AGAAATTAGT TATATTGTCA ATGTATTTGG AAGAGGAGAC 
ATCATTGTCC AGGCAACTGG CCGTGAAGGG GACACTGTCA CACTGCCAAC CTATATGAAT 
CACGCAACTT CCATTGAAAG AATCTCATGG ATGTTTCATG ACAAAGGGAA GAAAATACAC 
CTGGCTGAAT TCAGAAACCA CAAGTTTATC AGAGTAAATA AGAATTATTT CCCCAACCGT 
CTTGAAAAAT CGAATTATGG AACCGCATTG CAGATCAAAG AGCTGAGAAT GGAGGATTCT 
GGGATCTTCA CTGCACACAT TTATGCTAAT GGAAGAAAGA GAGACATTTC TTATATTCTT 
GTTGTGTTCG AGCTTATACC AACACCAGTA ATAGAAGTGA CGTATGATGA GAATTTTACT 
GATCTCTGCC ATCTTCATTG TTCTGTCAAT TCAAACTCAA CAACCCTGTC CTACAGTTGG 
ACCTACAGAG ACAATGGCAC TGACATACTA TATGCCAATG GCAGCACAAT CAGTATATTA 
CTAAAGAATA TGGCATCAGG CTCAGAATTT ACCTGCTTGG TACAAAATCC TGTTCACAGG 
AACAGTGCGT CAGTCCTTTT AAAGTCACTT GGTGGAAGAC GAACAGATAT ACGAAAAGAG 
ATACAAACTG AGATACGACA AGGAATACAA AGAGAGATAC GGCCGGAGAT ACAAACTGAG 
ATACGACCAG GGATACAAAG AGAGATACAG CCGGAGATAC AAACTGAGAT ACGAACAGGG 
ATACAAAGAG AGATACAGCC GGAGATACAA ACTGAGATAC GACCAGGGAT ACAAAGAGAG 
ATACAGCCGG AGATACAAAC TGAGATACGA ACAGGGATAC AAAGAGAGAT ACAGCCGGAG 
ATACAAACTG AGATACGACC AGGGATACAA AGAGAGATAC AGCCGGAGAT ACAAACTGAG 
ATACGACCAG GGATACAAAG AGAGATACAG CCGGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAAAGAG AGATACAGCC GGAGATACAA AGAGAGATAC AGCCGGAGAT ACAAACTGAG 
ATACGACCAT GGATACAAAA AGAGATACAG CCGGAGATAC AAACAGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GGAGATACAA ACTGAGATAC GACCAGGAAT ACAAAAAGAG 
ACACGAAGAA AGATACATAA AGAGATAGAA CCAAATATAC AAACAGAGAT AGAAAAAGAA 
ATGCAACCAG ACATACGACC AGAAATACAT ACAGAGATAG AAAAAGATAT TCAACCAGAG 
ATACAATCAG AAACACCGAG AGAGGCACAA ATAAAAGGAC CGAAAAAAGA TTCTCGTATA 
CTGACAGTAA TAATTGCAGT TTCGATACTG TGCGGAATAT TTGTTGGTAT AGCTCTTTAT 
TATGGAATAA GACGACGACT AAAGGTTAAA AATCAGTCTA ATTGCACTAT TACATACAGT 
GAAGTTCTGA CAAGTACTCA GGATAAAGAG CATATTAAAG ACTCTATTAC TTACAGTGAA 
GTTCAGATAA ATACTCAGGA TAAAGTGCAT ACTAAAGAGA CTATTGCATA CAGTGAATTT 
CAGGGAAATT CTAGGAGAAA TATGCAGGGC AAAGAAGAAC CTGATTATAA TGATTATACT 
GTTACATTCA GTGAAGGTCA GAGAAATACA CAGGATAAAG AGCATATTAA AGACTCTATT 
ACTTACAGTG AAGTTCAGAT AAATACTCAG GATAAAGTGC ATACTAAAGA CACTATTACA 
TACAGTGAAG TTCAGATGAA TTCTAGGAGA AATATGCAGG GCAAAGAAGA ATCTGATTAT 
AATGATTATA CTGTTACATT CAGTGAAGGT CAGAGAAATA CACAGAATAA AAAACAGTCT 
GATGATACTA TTATATACAG TAAAGTTCAG ATAAATACTC AGGATAAAGA GCATATTAAA 
GACTCTATTA CTTACAGTGA AGTTCAGATA AATACTCAGG ATAAAGTGCA TACTAAAGAC 
ACTATTACAT ACAGTGATGT TCTGATGAAT TCTAGGAGAA ATATGCAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGACATT CTCAGAATTT AGAAAATGTT 
GATCAGTCTG CATCTAACAA GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATATAA AAATGTCTCT GGAGTCTCAA TCTCAGCCTC TGCATCATCT 
GCATCATCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAATAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GGAGTCTCCA TCTCAGCCTC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AACAGTGTTA AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCTCAGAGA AATTCTCAGG ATAAAGAACA AACTAAAGAA 
ACTATTACAT ACAGGGAAAG CCAGATAAAT ACTCAGAATT TAGAAAATGT TGGCCGGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GTATCTGGTG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTT TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC AGGAGGCTCC ATCTCAGCCT CTGCATCATC CTCTTCTGGA 
TACCTTGCAT CCTCAATGGA AAGCAGTGTT AAGGATAAAG AGCAAACCAA AGACACTTTT 
ACATACATTG AAGCTCAGAT AAATTCTCAG GGCAAAGAGC AAACTAAAGA TGTTATTACA 
TACAGTGAAG CTCAGAGAAA TTTTCAGGAT AAAGAGCAAC CTAAAGAGAG TATTACATGT 
AGTGAAGTTT ATAGAAGTAC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC TGATGCTCCA AGCTTTCCAT CATCCATCTG TTCCCCTCCT 
GTATATCCTG TGTCTCCAAC AGAAAACAGT CAACAGGATA AAAAGCAAAC TAAAGACTCT 
ATTACCTACA GTGTTGGTCA GATGAATACT CAGGATAAAA AGCAAACTCT ATTAACCTAC 
AGTGTTGGTC AGATAAAGTC TCAGGATATA AAGCAAACTA AAGACACTGT TACATATAGA 
GAAGGCCACA TAAACGCGCA GGATGAACAG CAAATGAAAG ACTCCATTAC ATACAGTGAA 
GCCAAGATAA GTACTCGGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCGTCTT GGATACTTTG GAGCAAGTCT GTATGGGACA 
TCTTCACATC TGCCCTGGCT TGCTGGATTA ATTGAAGCTC CAAGTCTATG TAACACTTGC 
TCCAGAGTCT CCATCCCAAT CTCAGCATTA TGTGCCTGTG CCTCTTCTGG ATCCCATATG 
TCCCATTGGG ATAGCAGTCA TAAGGAGAAC AATCAAACTA AAGACATGAT TACATACAGT 
GAAGTTCAGA CAAGTATGCA GGATGAACAA CAAGCTAAAG GCACTATTAC ATACAGTGAT 
GGTCAGGTAA AAACTCATGT GTTTGCAGAA CAAAAGTCCA ATCACCAAGG CACCAGAAGC 
ATCAGACAAG AAGGTGGTCA GATGTCAGCG CTGATCAAGA ATAGCGAAAT ATCTGAAGAC 
AATATTACAG ATGATATAAG ATCTCTGTTA AGGCCTCATC CTGAGTGTCC AGTTCAGTTT 
TCGGGCCCAG CCCTTATAAA GGATGTTAGT GAGCTGGAGA GAGTGCAGTG CCATTCAACT 
AAACTGGTAA AAGAGATGGG AGAATTTAGC TATGAGGAAA GACAATCAAG TGCAAGCTTT 
CCCCTGCAGC TCCCATTCAA ACAGGGAGTT TCCAAAGACT TGGAGGACAT CACTTCAACC 
TATGTCTATA GCCCTGGGAT TCATACTGCA TGCATAGGCA ATAACAATGT TCTGAAGTCT 
GTGTATACGT ATAAAGGTTT GCAAACTGAC AGCCTTCATG CTGATATCAT GAATGGTAAT 
GAGTGCAAAT GCACAGAAGA GACGGCCAAT CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891107.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746967.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X2, mRNA.

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CloneID	OXa64376
Clone ID Related Accession (Same CDS sequence)	XM_031891107.1 , XM_031891106.1
Accession Version	XM_031891107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGATCTCTG 
TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG TTTTCGGGCC CAGCCCTTAT AAAGGATGTT 
AGTGAGCTGG AGAGAGTGCA GTGCCATTCA ACTAAACTGG TAAAAGAGAT GGGAGAATTT 
AGCTATGAGG AAAGACAATC AAGTGCAAGC TTTCCCCTGC AGCTCTCATT CAAACAGGGA 
GTTTCCAAAG ACTTGGAGGA CATCACTTCA ACCTATGTCT ATAGCCCTGG GATTCATACT 
GCATGCATAG GCAATAACAA TGTTCTGAAG TCTGTGTATA CGTATAAAGG TTTGCAAACT 
GACAGCCTTC ATGCTGATAT CATGAATGGT AATGAGTGCA AATGCACAGA AGAGACCAGC 
AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746967.1
CDS	264..10007
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891107.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891107.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGATCTCTG 
TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG TTTTCGGGCC CAGCCCTTAT AAAGGATGTT 
AGTGAGCTGG AGAGAGTGCA GTGCCATTCA ACTAAACTGG TAAAAGAGAT GGGAGAATTT 
AGCTATGAGG AAAGACAATC AAGTGCAAGC TTTCCCCTGC AGCTCTCATT CAAACAGGGA 
GTTTCCAAAG ACTTGGAGGA CATCACTTCA ACCTATGTCT ATAGCCCTGG GATTCATACT 
GCATGCATAG GCAATAACAA TGTTCTGAAG TCTGTGTATA CGTATAAAGG TTTGCAAACT 
GACAGCCTTC ATGCTGATAT CATGAATGGT AATGAGTGCA AATGCACAGA AGAGACCAGC 
AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891106.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746966.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X1, mRNA.

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CloneID	OXa64376
Clone ID Related Accession (Same CDS sequence)	XM_031891107.1 , XM_031891106.1
Accession Version	XM_031891106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGATCTCTG 
TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG TTTTCGGGCC CAGCCCTTAT AAAGGATGTT 
AGTGAGCTGG AGAGAGTGCA GTGCCATTCA ACTAAACTGG TAAAAGAGAT GGGAGAATTT 
AGCTATGAGG AAAGACAATC AAGTGCAAGC TTTCCCCTGC AGCTCTCATT CAAACAGGGA 
GTTTCCAAAG ACTTGGAGGA CATCACTTCA ACCTATGTCT ATAGCCCTGG GATTCATACT 
GCATGCATAG GCAATAACAA TGTTCTGAAG TCTGTGTATA CGTATAAAGG TTTGCAAACT 
GACAGCCTTC ATGCTGATAT CATGAATGGT AATGAGTGCA AATGCACAGA AGAGACCAGC 
AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746966.1
CDS	441..10184
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891106.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891106.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGATCTCTG 
TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG TTTTCGGGCC CAGCCCTTAT AAAGGATGTT 
AGTGAGCTGG AGAGAGTGCA GTGCCATTCA ACTAAACTGG TAAAAGAGAT GGGAGAATTT 
AGCTATGAGG AAAGACAATC AAGTGCAAGC TTTCCCCTGC AGCTCTCATT CAAACAGGGA 
GTTTCCAAAG ACTTGGAGGA CATCACTTCA ACCTATGTCT ATAGCCCTGG GATTCATACT 
GCATGCATAG GCAATAACAA TGTTCTGAAG TCTGTGTATA CGTATAAAGG TTTGCAAACT 
GACAGCCTTC ATGCTGATAT CATGAATGGT AATGAGTGCA AATGCACAGA AGAGACCAGC 
AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891108.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746968.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X3, mRNA.

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CloneID	OXa64377
Clone ID Related Accession (Same CDS sequence)	XM_031891108.1
Accession Version	XM_031891108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GGAGGAGAAA TCCAAGTTAT TGGGCGTTTA GGGGAGACTG TCACACTGCA GTGCCCTCTG 
AATTCACCAG CAAAAATCTC ATGGATGTTT GATGATAATG GGGAAAAAAT ACTTTTGGCT 
GAATTCAGAG ACCAGAAGTT CATCAGAGTG AATGGGAATT ATTTCTCCAG GCGACTGGAA 
GGATCTAACA GTGGCACCGC ATTAAGGATC AAACATCTGA GAATGGAGGA TGGTGGAGTG 
TTCACTGCTC ACATCACTGT TAATGGAAGC GCTACAGACA TTAGTTATGT TCTCACAGTG 
TTGGGAAGAG GAGACATCAT TCTGCCAGTG ACTGGCCTTG TAGGGGGGAA TGTCACATTG 
CCAACCTATC TGACTCTGCC AGCTGCCATC GAAAAAATCT CATGGATGTT TCATGACAAA 
GGCAAAAAAA TACACCTGGC TGAATTAAGA AACCAGAAGT TTAGCAGACT GAACAAGAAT 
TATTTCTACA ACCGCCTTGA AAAATCTAAT GATGGAACCG CATTACAGAT CAGAGAGCTG 
AGAATGGAGG ATTCTGGGAT CTTCACTGCA CACATTCATT TATATGGACG AAAAAGAGAC 
ATTTCTTATA TTCTAGCCGT GTTCGAGCCT ATCCCAACAC CAGTGATAAA AGTGACGTTT 
GATGAGAATT CCACTGATCT CTGCCATCTT CATTGTTCTG TCCCATCAAA CTCACCAACC 
CTGTCCTACA GTTGGACCTA CAGAGATACT GGCACTGACA GACTATATGC CAATGGCAGC 
ACAATCACTG TATCACTGAA GGATAAGGCA TTGGGGGCAG AATTTACCTG CTGGGTACGA 
AATCCTGCTC ACAGCAATAG TGTGTCAGTC GTTTTAAATT CACTTGGTGG GAGACAAACA 
GAGATACGAA AAGAGATACA ACCAGGGATG CATAAAGAGA TACAATTAAA GATAGAAACA 
GAGGTACGAC CAGGGATACA AACAGAGCTA CAGCCAAAGA TACAAACAGA GATACGACCA 
GGGATACAAA CAGAGATACG AACAAAGATA CCAACAGAGC TACAACCAGA GATAAAAACT 
AAGCTAGAAC CAGGGATACA AATAGATCTA CAACCAAAGA TACAAACAGA GCTACAACCA 
AAGATAAAAA CAGAGATACG ACCAGGGATA CAAACAGAGA TACGAACAAA GATACAAACA 
GAGATACAAC CAAAGATAAA AACAGAGATA CAACCAGGAA TACAAACAGA GATACGACCA 
GGAATACAAA CAGAGATACG GCCAGGAATA CAAACAGAGA TACGGCCAGG AATACAAACA 
GAGATACAAA CTAAGCTAGA ACCAGGGATA CAAATAGATC TACAACCAAA GATACAAACA 
GAGATACAAC CAAAGATAAA AACAGAGATA CGACCAGGAA TACAAACAGA GATACGAACA 
AAGATACAAA CAGAGCTACG ACCAGAGATA AAAACTAAGC TAGAACCAGG GATACAAACA 
GAGCTACAAC CAAAGATACA AACAGAGATA CAACCAAAGA TACAAACGGA GATACAACCA 
AAGATACAAA CAGAGATACA ATCAAAGATA CAAACGGAGA TACAACCAAG GATACAAACA 
GAGATACAAC CAAAGATACA AACAGAGATA CAACCAAGGA TACAAACAGA GATACAACCA 
AGGATACAAA CAGAGATACT ACCGGAGTTA CGAACAACGA TAAAAACAGA CACAAAATCA 
GAAATACAAA CAGACATAAA GATACAAACT GAGGAAAAAA TAAAAGCAGG AGGAGAAATC 
CAAGTTATTG GGCGTTTAGG GGAGACTGTC ACACTGCAGT GCCCTCTGAA TTCACCAGCA 
AAAATCTCAT GGATGTTTGA TAATAATGGG GAAAAAATTC TCCTGGCTGA ATACAGAGAC 
CAGAAGTTCA TCAGAGTGAA TAACAACTAC TTCATCAACC GGCTGGAAGG GTCTAACAGT 
GGCACCGCAT TAAGGATCAA ACATCTGAGA ATGGAGGATG GTGGGGTCTT CACTGCTCAC 
ATCACTGTTA ATGGAAGCGC TACAGAAATT ATTTATGTTC TCACAGTGTT GGGAAGAGGA 
GACATCATTC TGCCAGTGAC TGGCCTTGTA GGGGGGAATG TCACACTGCC AACCTATCTG 
ACTCTGCCAG CTGCCATCGA AAGAATCTCA TGGATGTTTC ATGACAAGGG GAAGCGAATA 
CACCTGGCTG AATTCAGACA CCAGAAGTTT ATCACAGTTA ATAAGAAATA TTTCTTAAAC 
CGCCTTGAAA AACCTAATGA TGGAACCGCA TTAAGGATCA GAGAGCTGAG AATGGAGGAT 
TCTGGGATCT TCACTGCACA CATTCATTTT AATGGACGAA AAAGAGACAT TTCTTACATT 
CTAGCCGTTT TCGAGCCTAT CCCAACACCA GTGATAAAAG TGACGTTTGA TGAGAATTCC 
CCTGATCTCT GCCATCTTCA TTGTTCTGTC CCATCAAACT CACCAACCCT GTCCTACAGT 
TGGACCTACA GAGATACTGG CAATGACAGA CTATATGCCA ATGGCAGCAC AATCACTGTA 
TCACTGAAGG ATAAGGCATT GGGGGCAGAA TTTACCTGCT GGGTACAGAA TCCTGCTCAC 
AGGAACAATG CATCAGTCCC TTTAAAGTCA CTTCATGGGA GACGAACAGG AATACAAACT 
GAGATACAAC CATGGATACA AAGAAAGATA CAATCAAAGA TACAAAAAGA GATACGACCA 
GATATAAAAG CAGAGATTCA ACATAAAATA CAAACAGACA CAAAACTAGG AACGCAGACA 
GAGATACAAC CTGGAACAGA GATACATCCA GAAATACAAC CAGAGATACA AATAGAGCTA 
CGACCCAGGA TACAAACAGA GATACAACCA GATATAAAAG CAGAGATACG ACCAAAGATA 
CAAGCAGGGA TACACACTGA GCTACAACCA AAGATAAAAA CAGAGATACG ACCAGGGACA 
CAAACAGAGC TACGACCAGA GATAAAAACA GAGCTACGAC CAGAGATAAA AACAGAGCTA 
CGACCAGAGA TAAAAACAGA GATACGACCA GGGACACAAA CAGAGCTACG ACCAGAGATA 
AAAACAGAGC TACGACCAGA GATAAAAACA GAGCTACGAC CAGGGATAAA AACAGAGATA 
CGACCAGGGA CACAAACAGA GATACGACCA GAGATAAAAA CAGAGCTACG ACCAGAGATA 
AAAACAGAGA TACGACCAGG GACACAAACA GAGCTGCGAC CAGGGATACA AACAGAGCTA 
CGACCAGAGA TAAAAACAGA GCTACGACCA GAGATAAAAA CAGAGCTTCG ACCAGAGATA 
AAAACAGAGC TACGACCAGA GATAAAAACA GAGATACAAC AAGGGATACA AACAGAGCTA 
CGTCCAGAGA TAAAAACTGA GCTAGTACCA GGGATACGTA CAGATATACA ACCAGGGATA 
CAAACAGATC TACGAACAGA GATACAAAAA AAGATACAAA CAGACACCAA ACCAGAAATA 
CTAACAGAGG TGCAAACAGA CATAAAGAGA CAAACAAAGG CAAAAATAAA AGCAGGAGGA 
GACATCCAGG TAATTGGCCG TGTAGGGGAG ACTGTCACAC TGCAGTGCCC TCTGAACCTA 
CCAACACGAA TCTTGTGGAC GTATGATGAT AATGGGGAAA AAATACTCCT GGCTGAATAC 
AGAGACCAGA AATTTATCAG AGTGAATAAC AACTACTTCA TCAACCGGCT GGAAGGGTCT 
AACAGTGGCA CCGCATTACT GATCAGAGAG CTAAAAAGAG AGGATAGTGG GGTCTTCACT 
GCTCACATTA CTGTTAATGG AAGCGCTACA GACATTAGTT ATATACTGAA TGTACTGGGA 
AGAGGAGACA TCATTCTGCC AGTGACTGGC CTTGTAGGGG GGAATGTCAC ACTGCCAACC 
TATCTGACTC TGCCAGCTGC CATCGAAAGA ATCTCATTGA TGTTTCATGA CAAAGGCAAA 
AAAATACACC TGGCTGAATT AAGAAACCAT AAGTTTAGCA GACTGAACAA GGATTATTTC 
TACAACCGCC TTGAAAAATC TAATGATGGA ACCGCATTAC AGATCAGGGA GCTGAGAATG 
GAGGATTCTG GGATCTTCAC TGCACACATT CATGTTAATG GACAAAAAAG AGACATTTCT 
TATATTCTAG CCGTGTTCGA GCCTATCCCA ACACCAGTAA TAGAAGTGAC ATCTGATGAG 
AATTTCCCTG ATCTCTGCCA TCTTCATTGT TCTGTCCCAT CAAACTCACC AACCCTGTCC 
TACAGTTGGA CCTACAGAGA TACTGGCACT GACAGACTAT ATGCCAATGG CAGCACAATC 
ACTGTATCAC TGAAGGATAA GGCATTGGGG GCAGAATTTA CCTGCTGGGT ACAGAATCCT 
GCTCACAGGA ACAGTGTGTC AGTCGTTTTA AAGTCATATA GTGGGAGACA AACAGGAATA 
CAACCAAAGA TACGACCAGA GATACAACAA AAAATACAAA CGGAGACACA ACCAGAGATA 
AAAACAGAGA TACGACCAGG GATAAAAACA GAGATACAAC CAGAGATAAA AACAGAAATA 
CAGCCAGGGA TAAAAACAGA GAAACAACCA GGGATACTAA CGGAGATACG ACCAGGGATA 
AAAACAGAGA TGCGACCAGG GGTGCAAAAA GAGATACAAC TAGGAATACA AAAAGAAATA 
CGATCAGAGA TACAAACTGA GATACGACAA GGGATACAAA GAGAGACACA AAAAGAGACA 
GAAATAAAAG CAGGAGGAGA CATCCAGGTG ATTGGCCGTG TAGGGGAGAC TGTCACACTG 
CAGTGCCCTC TGAATCTACC AACAAGAATC TTGTGGACGT ATGATGATAA TGGGGAAAAA 
ATACTCCTGG CTGAATACAG AGACCAGAAG TTTATCAGAG TGAATAACAA CTACTTCATC 
AACCGGCTGG AAGGATCTAA CAGTGGCACT GCATTAAGGA TCAAAAACCT GAGAATGGAG 
GATGGTGGGG TCTTCACTGC TCACATCACT GTTAATGGAA GCGCTACAGA AATTAGTTAT 
ATTGTCAATG TATTTGGAAG AGGAGACATC ATTGTCCAGG CAACTGGGCG TGAAGGGGAC 
ACTGTCACAC TGCCAACCTA TATGAATCAC GCAACTTCCA TTGAAAGAAT CTCATGGATG 
TTTCATGACA AAGGGAAGAA AATACACCTG GCTGAATTCA GAAACCACAA GTTTATCAGA 
GTAAATAAGA ATTATTTCCC CAACCGTCTT GAAAAATCGA ATTATGGAAC CGCATTGCAG 
ATCAAAGAGC TGAGAATGGA GGATTCTGGG ATCTTCACTG CACACATTTA TGCTAATGGA 
AGAAAGAGAG ACATTTCTTA TATTCTTGTT GTGTTCGAGC TTATACCAAC ACCAGTAATA 
GAAGTGACGT ATGACGAGAA TTTTACTGAT CTCTGCCATC TTCATTGTTC TGTCAATTCA 
AACTCAACAA CCCTGTCCTA CAGTTGGACC TACAGAGACA ATGGCACTGA CATACTATAT 
GCCAATGGCA GCACAATCAG TATATTACTA AAGAATATGG CATCAGGCTC AGAATTTACC 
TGCTTGGTAC AAAATCCTGT TCACAGGAAC AGTGCGTCAG TCCTTTTAAA GTCACTTGGT 
GGAAGACGAA CAGATATACG AAAAGAGATA CAAACTGAGA TACGACAAGG AATACAAAGA 
GAGATACAGC CGGAGATACA AACTGAGATA CGACCAGGGA TACAAAGAGA GATACAGCCG 
GAGATACAAA CTGAGATACG AACAGGGATA CAAAGAGAGA TACAAACTGA GATACGACCA 
GGGATACAAA GAGAGATACA GCCGGAGATA CAAAGAGAGA TACAGCCGGA GATACAAACG 
GAGATACAAA CAGAGATACG ACAAGGGATA CAAAGAGAGA TACAGCCGGA GATACAAACG 
GAGATACAAA CAGAGATACG ACAAGGGATA CAGAGAGAGA TACAGCCGGA GATACAAACA 
GAGATACGAC CAGGGATACA GAGAGAGATA CAGCCAAAGA TACAAACAGA GATACAAACT 
GAGATACGAC CAGGGATACA GAGAGAGATA CAGCCGGAGA TACAAACTGA GATACGACCA 
GGGATACAGA GAGAGATACA GCCAGAGATA CAAACTGAGA TACGACCAGG GATACAAAAA 
GAGATACAGC CGGAGATACA AACAGAGATA CAAACTGAGA TACGACCAGG GATACAAAAA 
GAGACACGAC GAAAGATACA TAAAGAGATA GAACCAAATA TACAAACAGA GATAGAAAAA 
GAAATGCAAC CAGACATACG ACCAGAAATA CATACAGAGA TAGAAAAAGA TATTCAACCA 
GAGATACAAT CAGAAACACC GAGAGAGGCA CAAATAAAAG GACCGAAAAA AGATTCTCGT 
ATACTGACAG TAATAATTGC AGTTTCGATA CTGTGCGGAA TATTTGTTGG TATAGCTCTT 
TATTATGGAA TAAGACGACG ACTAAAGGTT AAAAATCAGT CTAATTGCAC TATTACATAC 
AGTGAAGTTC TGACAAGTAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACATACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAC 
GTTCAGATAA ATTCTAGGAG AAATATGCAG GGCAAAGAAG AACCTGATTA TAATGATTAT 
ACTATTACAT TCAGTGAAGG TCAGAGACAT ACACAGAATA AAAAACAGTC TGATGATGCT 
ATTACATACA GTAAAGTTCA GATAAATACT CAGGATAAAG AGCATATTAA AGACTCTATT 
ACTTACAGTG ACGTTCAGAT AAATACTCAG GATAAAGTGC ATACTAAAGA CACTATTACA 
TACAGTGACG TTCTGATGAA TTCTAGGAGA AATATGCAGG ATAAAGAGCA AACTAAAGGC 
ACTATTACAT ACACTGAAGC GCAGAGACAT TCTCAGAATT TAGAAAATGT TGACCAGTCT 
GCATCTAACA AGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCATCC 
TCTTCTGGAT ACCTTGTATC CCCAATGGAA AGCAGTCTTA AGAATTTAGA AAGTGTTGGT 
CAGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGAAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCT CAGCCTCTGC ATCATCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCCA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGTCAGT CTGCTTCTAA CAAGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCTGGAGTCT CCATCCCAGC CTCTGCATCG 
TCCTCTTCTG GATACCTTGC ATCCCTAATG GAAAGCAGTG TTAAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCGCAG AGAAATTCTC AGGATAAAGA ACAAACTAAA 
GAAACTATTA CATACAGGGA AAGCCAGATA AATACTCAGA ATTTAGAAAA TGTTGGCCGG 
TCTGCCTCTA ACAAGCAAAA TGGGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT GTCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCAGGAGGC TCCATCTCAG CCTCTGCATC ATCTGCATTG 
TCCTCTTCTG GATACCTTGC ATCCTCAATG GAAAGCAGTG TTAAGGATAA AGAGCAAACC 
AAAGACACTT TTACATACAT TGAAGCTCAG ATAAACTCTC AGGGCAAAGA GCAAACTAAA 
GATATTATTA CATACAGTGA AGCTCAGAGA CATTTTCAGG ATAAAGAGCA ACCTAAAGAG 
AGTATTACAT GTAGTGAAGT TTATAGAAGT ACTCAGGATA AAGAGCAAAC TAAAGACACA 
ATTACATACA GTAAAGTTCA GAGAAATATG CAGAATCTAG AAAATGTTGG TCGGTCTGCC 
CCTAACAGGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTAGGAAA CACTGGACCA 
AGTCTGTATG GGGCTTCTTT AGCAGGATTA TCCGAAGCTC CAAGCTTTCC ATCATCCATC 
TCTTCCCCTC CTGTATACCC TGTGTCTCCA ACAGAAAACA GTCAACGGGT AACCTCAGCA 
GCAATTGATA AAAAGCAAAC TAAAGACTCT ATTACCTACA GTGTTGGTCA GATGAATACT 
CAGGATAAAA AGCAAACTCT ATTAACCTAC AGTGTTGGTC AGATAAAGTC TCAGGATATA 
AAGCAAACTA AAGACACTGT TACATATAGA GAAGGCCACA TAAACGCACA GGATGAACAG 
CAAATGAAAG ACTCCATTAC ATACAGCGCA GCCAAGATAA GTACTCAGAA TCTAGAAAAT 
GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT GTGATGAGCA GTGTATCTGG GGATCGTCTT 
GGATACTTTG GAGCAAGTCT GTATGGGACC TCTTCACATC TGCCCTGGCT TGCTGGATTA 
ATTGAAGCTC CAAGTCTATG TAACACTTGC TCCAGAGTCT CCATCCCAAT CTCAGCATTA 
TGTGCCTGTG CCTCTTCTGG ATCCCATATG TCCCATTGGG ATAGCAGTCA TAAGGAGAAC 
AATCAAACTA AAGACATGAT TACATACAGT GAAGTTCAGA CAAGTATGCA GGATGAACAA 
CAAGCTAAAG GCACTATTAC ATACAGTGAT GGTCAGGTAA AAACTCATGT GTTTGCAGAA 
CAAAAGTCCA ATCACCAAGG CACCAGGAGT ATCAGACAAG AAGGTGGTCA GATGTCAGCG 
CTGATCAAGA ATAGCGAAAT ATCTGAAGAC AATATTACAG ATGATATAAG ATCTCTGTTA 
AGGCCTCATC CTGAGTGTCC AGTTCAGTTT TCGGGCCCAG CCCTTATAAA GGATGTTAGT 
GAGCTGGAGA GAGTGCAGTG CCATTCAACT AAACTGGTAA AAGAGATGGG AGAATTTAGC 
TATGAGGAAA GACAATCAAG TGCAAGCTTT CCCCTGCAGC TCTCATTCAA ACAGGGAGTT 
TCCAAAGACT TGGAGGACAT CACTTCAACC TATGTCTATA GCCCTGGGAT TCATACTGCA 
TGCATAGGCA ATAACAATGT TCTGAAGTCT GTGTATACGT ATAAAGGTTT GCAAACTGAC 
AGCCTTCATG CTGATATCAT GAATGGTAAT GAGTGCAAAT GCACAGAAGA GACCAGCAAT 
CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746968.1
CDS	441..10181
Translation	MKKFTCDILFLLWTSFLITCGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891108.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891108.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GGAGGAGAAA TCCAAGTTAT TGGGCGTTTA GGGGAGACTG TCACACTGCA GTGCCCTCTG 
AATTCACCAG CAAAAATCTC ATGGATGTTT GATGATAATG GGGAAAAAAT ACTTTTGGCT 
GAATTCAGAG ACCAGAAGTT CATCAGAGTG AATGGGAATT ATTTCTCCAG GCGACTGGAA 
GGATCTAACA GTGGCACCGC ATTAAGGATC AAACATCTGA GAATGGAGGA TGGTGGAGTG 
TTCACTGCTC ACATCACTGT TAATGGAAGC GCTACAGACA TTAGTTATGT TCTCACAGTG 
TTGGGAAGAG GAGACATCAT TCTGCCAGTG ACTGGCCTTG TAGGGGGGAA TGTCACATTG 
CCAACCTATC TGACTCTGCC AGCTGCCATC GAAAAAATCT CATGGATGTT TCATGACAAA 
GGCAAAAAAA TACACCTGGC TGAATTAAGA AACCAGAAGT TTAGCAGACT GAACAAGAAT 
TATTTCTACA ACCGCCTTGA AAAATCTAAT GATGGAACCG CATTACAGAT CAGAGAGCTG 
AGAATGGAGG ATTCTGGGAT CTTCACTGCA CACATTCATT TATATGGACG AAAAAGAGAC 
ATTTCTTATA TTCTAGCCGT GTTCGAGCCT ATCCCAACAC CAGTGATAAA AGTGACGTTT 
GATGAGAATT CCACTGATCT CTGCCATCTT CATTGTTCTG TCCCATCAAA CTCACCAACC 
CTGTCCTACA GTTGGACCTA CAGAGATACT GGCACTGACA GACTATATGC CAATGGCAGC 
ACAATCACTG TATCACTGAA GGATAAGGCA TTGGGGGCAG AATTTACCTG CTGGGTACGA 
AATCCTGCTC ACAGCAATAG TGTGTCAGTC GTTTTAAATT CACTTGGTGG GAGACAAACA 
GAGATACGAA AAGAGATACA ACCAGGGATG CATAAAGAGA TACAATTAAA GATAGAAACA 
GAGGTACGAC CAGGGATACA AACAGAGCTA CAGCCAAAGA TACAAACAGA GATACGACCA 
GGGATACAAA CAGAGATACG AACAAAGATA CCAACAGAGC TACAACCAGA GATAAAAACT 
AAGCTAGAAC CAGGGATACA AATAGATCTA CAACCAAAGA TACAAACAGA GCTACAACCA 
AAGATAAAAA CAGAGATACG ACCAGGGATA CAAACAGAGA TACGAACAAA GATACAAACA 
GAGATACAAC CAAAGATAAA AACAGAGATA CAACCAGGAA TACAAACAGA GATACGACCA 
GGAATACAAA CAGAGATACG GCCAGGAATA CAAACAGAGA TACGGCCAGG AATACAAACA 
GAGATACAAA CTAAGCTAGA ACCAGGGATA CAAATAGATC TACAACCAAA GATACAAACA 
GAGATACAAC CAAAGATAAA AACAGAGATA CGACCAGGAA TACAAACAGA GATACGAACA 
AAGATACAAA CAGAGCTACG ACCAGAGATA AAAACTAAGC TAGAACCAGG GATACAAACA 
GAGCTACAAC CAAAGATACA AACAGAGATA CAACCAAAGA TACAAACGGA GATACAACCA 
AAGATACAAA CAGAGATACA ATCAAAGATA CAAACGGAGA TACAACCAAG GATACAAACA 
GAGATACAAC CAAAGATACA AACAGAGATA CAACCAAGGA TACAAACAGA GATACAACCA 
AGGATACAAA CAGAGATACT ACCGGAGTTA CGAACAACGA TAAAAACAGA CACAAAATCA 
GAAATACAAA CAGACATAAA GATACAAACT GAGGAAAAAA TAAAAGCAGG AGGAGAAATC 
CAAGTTATTG GGCGTTTAGG GGAGACTGTC ACACTGCAGT GCCCTCTGAA TTCACCAGCA 
AAAATCTCAT GGATGTTTGA TAATAATGGG GAAAAAATTC TCCTGGCTGA ATACAGAGAC 
CAGAAGTTCA TCAGAGTGAA TAACAACTAC TTCATCAACC GGCTGGAAGG GTCTAACAGT 
GGCACCGCAT TAAGGATCAA ACATCTGAGA ATGGAGGATG GTGGGGTCTT CACTGCTCAC 
ATCACTGTTA ATGGAAGCGC TACAGAAATT ATTTATGTTC TCACAGTGTT GGGAAGAGGA 
GACATCATTC TGCCAGTGAC TGGCCTTGTA GGGGGGAATG TCACACTGCC AACCTATCTG 
ACTCTGCCAG CTGCCATCGA AAGAATCTCA TGGATGTTTC ATGACAAGGG GAAGCGAATA 
CACCTGGCTG AATTCAGACA CCAGAAGTTT ATCACAGTTA ATAAGAAATA TTTCTTAAAC 
CGCCTTGAAA AACCTAATGA TGGAACCGCA TTAAGGATCA GAGAGCTGAG AATGGAGGAT 
TCTGGGATCT TCACTGCACA CATTCATTTT AATGGACGAA AAAGAGACAT TTCTTACATT 
CTAGCCGTTT TCGAGCCTAT CCCAACACCA GTGATAAAAG TGACGTTTGA TGAGAATTCC 
CCTGATCTCT GCCATCTTCA TTGTTCTGTC CCATCAAACT CACCAACCCT GTCCTACAGT 
TGGACCTACA GAGATACTGG CAATGACAGA CTATATGCCA ATGGCAGCAC AATCACTGTA 
TCACTGAAGG ATAAGGCATT GGGGGCAGAA TTTACCTGCT GGGTACAGAA TCCTGCTCAC 
AGGAACAATG CATCAGTCCC TTTAAAGTCA CTTCATGGGA GACGAACAGG AATACAAACT 
GAGATACAAC CATGGATACA AAGAAAGATA CAATCAAAGA TACAAAAAGA GATACGACCA 
GATATAAAAG CAGAGATTCA ACATAAAATA CAAACAGACA CAAAACTAGG AACGCAGACA 
GAGATACAAC CTGGAACAGA GATACATCCA GAAATACAAC CAGAGATACA AATAGAGCTA 
CGACCCAGGA TACAAACAGA GATACAACCA GATATAAAAG CAGAGATACG ACCAAAGATA 
CAAGCAGGGA TACACACTGA GCTACAACCA AAGATAAAAA CAGAGATACG ACCAGGGACA 
CAAACAGAGC TACGACCAGA GATAAAAACA GAGCTACGAC CAGAGATAAA AACAGAGCTA 
CGACCAGAGA TAAAAACAGA GATACGACCA GGGACACAAA CAGAGCTACG ACCAGAGATA 
AAAACAGAGC TACGACCAGA GATAAAAACA GAGCTACGAC CAGGGATAAA AACAGAGATA 
CGACCAGGGA CACAAACAGA GATACGACCA GAGATAAAAA CAGAGCTACG ACCAGAGATA 
AAAACAGAGA TACGACCAGG GACACAAACA GAGCTGCGAC CAGGGATACA AACAGAGCTA 
CGACCAGAGA TAAAAACAGA GCTACGACCA GAGATAAAAA CAGAGCTTCG ACCAGAGATA 
AAAACAGAGC TACGACCAGA GATAAAAACA GAGATACAAC AAGGGATACA AACAGAGCTA 
CGTCCAGAGA TAAAAACTGA GCTAGTACCA GGGATACGTA CAGATATACA ACCAGGGATA 
CAAACAGATC TACGAACAGA GATACAAAAA AAGATACAAA CAGACACCAA ACCAGAAATA 
CTAACAGAGG TGCAAACAGA CATAAAGAGA CAAACAAAGG CAAAAATAAA AGCAGGAGGA 
GACATCCAGG TAATTGGCCG TGTAGGGGAG ACTGTCACAC TGCAGTGCCC TCTGAACCTA 
CCAACACGAA TCTTGTGGAC GTATGATGAT AATGGGGAAA AAATACTCCT GGCTGAATAC 
AGAGACCAGA AATTTATCAG AGTGAATAAC AACTACTTCA TCAACCGGCT GGAAGGGTCT 
AACAGTGGCA CCGCATTACT GATCAGAGAG CTAAAAAGAG AGGATAGTGG GGTCTTCACT 
GCTCACATTA CTGTTAATGG AAGCGCTACA GACATTAGTT ATATACTGAA TGTACTGGGA 
AGAGGAGACA TCATTCTGCC AGTGACTGGC CTTGTAGGGG GGAATGTCAC ACTGCCAACC 
TATCTGACTC TGCCAGCTGC CATCGAAAGA ATCTCATTGA TGTTTCATGA CAAAGGCAAA 
AAAATACACC TGGCTGAATT AAGAAACCAT AAGTTTAGCA GACTGAACAA GGATTATTTC 
TACAACCGCC TTGAAAAATC TAATGATGGA ACCGCATTAC AGATCAGGGA GCTGAGAATG 
GAGGATTCTG GGATCTTCAC TGCACACATT CATGTTAATG GACAAAAAAG AGACATTTCT 
TATATTCTAG CCGTGTTCGA GCCTATCCCA ACACCAGTAA TAGAAGTGAC ATCTGATGAG 
AATTTCCCTG ATCTCTGCCA TCTTCATTGT TCTGTCCCAT CAAACTCACC AACCCTGTCC 
TACAGTTGGA CCTACAGAGA TACTGGCACT GACAGACTAT ATGCCAATGG CAGCACAATC 
ACTGTATCAC TGAAGGATAA GGCATTGGGG GCAGAATTTA CCTGCTGGGT ACAGAATCCT 
GCTCACAGGA ACAGTGTGTC AGTCGTTTTA AAGTCATATA GTGGGAGACA AACAGGAATA 
CAACCAAAGA TACGACCAGA GATACAACAA AAAATACAAA CGGAGACACA ACCAGAGATA 
AAAACAGAGA TACGACCAGG GATAAAAACA GAGATACAAC CAGAGATAAA AACAGAAATA 
CAGCCAGGGA TAAAAACAGA GAAACAACCA GGGATACTAA CGGAGATACG ACCAGGGATA 
AAAACAGAGA TGCGACCAGG GGTGCAAAAA GAGATACAAC TAGGAATACA AAAAGAAATA 
CGATCAGAGA TACAAACTGA GATACGACAA GGGATACAAA GAGAGACACA AAAAGAGACA 
GAAATAAAAG CAGGAGGAGA CATCCAGGTG ATTGGCCGTG TAGGGGAGAC TGTCACACTG 
CAGTGCCCTC TGAATCTACC AACAAGAATC TTGTGGACGT ATGATGATAA TGGGGAAAAA 
ATACTCCTGG CTGAATACAG AGACCAGAAG TTTATCAGAG TGAATAACAA CTACTTCATC 
AACCGGCTGG AAGGATCTAA CAGTGGCACT GCATTAAGGA TCAAAAACCT GAGAATGGAG 
GATGGTGGGG TCTTCACTGC TCACATCACT GTTAATGGAA GCGCTACAGA AATTAGTTAT 
ATTGTCAATG TATTTGGAAG AGGAGACATC ATTGTCCAGG CAACTGGGCG TGAAGGGGAC 
ACTGTCACAC TGCCAACCTA TATGAATCAC GCAACTTCCA TTGAAAGAAT CTCATGGATG 
TTTCATGACA AAGGGAAGAA AATACACCTG GCTGAATTCA GAAACCACAA GTTTATCAGA 
GTAAATAAGA ATTATTTCCC CAACCGTCTT GAAAAATCGA ATTATGGAAC CGCATTGCAG 
ATCAAAGAGC TGAGAATGGA GGATTCTGGG ATCTTCACTG CACACATTTA TGCTAATGGA 
AGAAAGAGAG ACATTTCTTA TATTCTTGTT GTGTTCGAGC TTATACCAAC ACCAGTAATA 
GAAGTGACGT ATGACGAGAA TTTTACTGAT CTCTGCCATC TTCATTGTTC TGTCAATTCA 
AACTCAACAA CCCTGTCCTA CAGTTGGACC TACAGAGACA ATGGCACTGA CATACTATAT 
GCCAATGGCA GCACAATCAG TATATTACTA AAGAATATGG CATCAGGCTC AGAATTTACC 
TGCTTGGTAC AAAATCCTGT TCACAGGAAC AGTGCGTCAG TCCTTTTAAA GTCACTTGGT 
GGAAGACGAA CAGATATACG AAAAGAGATA CAAACTGAGA TACGACAAGG AATACAAAGA 
GAGATACAGC CGGAGATACA AACTGAGATA CGACCAGGGA TACAAAGAGA GATACAGCCG 
GAGATACAAA CTGAGATACG AACAGGGATA CAAAGAGAGA TACAAACTGA GATACGACCA 
GGGATACAAA GAGAGATACA GCCGGAGATA CAAAGAGAGA TACAGCCGGA GATACAAACG 
GAGATACAAA CAGAGATACG ACAAGGGATA CAAAGAGAGA TACAGCCGGA GATACAAACG 
GAGATACAAA CAGAGATACG ACAAGGGATA CAGAGAGAGA TACAGCCGGA GATACAAACA 
GAGATACGAC CAGGGATACA GAGAGAGATA CAGCCAAAGA TACAAACAGA GATACAAACT 
GAGATACGAC CAGGGATACA GAGAGAGATA CAGCCGGAGA TACAAACTGA GATACGACCA 
GGGATACAGA GAGAGATACA GCCAGAGATA CAAACTGAGA TACGACCAGG GATACAAAAA 
GAGATACAGC CGGAGATACA AACAGAGATA CAAACTGAGA TACGACCAGG GATACAAAAA 
GAGACACGAC GAAAGATACA TAAAGAGATA GAACCAAATA TACAAACAGA GATAGAAAAA 
GAAATGCAAC CAGACATACG ACCAGAAATA CATACAGAGA TAGAAAAAGA TATTCAACCA 
GAGATACAAT CAGAAACACC GAGAGAGGCA CAAATAAAAG GACCGAAAAA AGATTCTCGT 
ATACTGACAG TAATAATTGC AGTTTCGATA CTGTGCGGAA TATTTGTTGG TATAGCTCTT 
TATTATGGAA TAAGACGACG ACTAAAGGTT AAAAATCAGT CTAATTGCAC TATTACATAC 
AGTGAAGTTC TGACAAGTAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACATACAGT 
GAAGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAC 
GTTCAGATAA ATTCTAGGAG AAATATGCAG GGCAAAGAAG AACCTGATTA TAATGATTAT 
ACTATTACAT TCAGTGAAGG TCAGAGACAT ACACAGAATA AAAAACAGTC TGATGATGCT 
ATTACATACA GTAAAGTTCA GATAAATACT CAGGATAAAG AGCATATTAA AGACTCTATT 
ACTTACAGTG ACGTTCAGAT AAATACTCAG GATAAAGTGC ATACTAAAGA CACTATTACA 
TACAGTGACG TTCTGATGAA TTCTAGGAGA AATATGCAGG ATAAAGAGCA AACTAAAGGC 
ACTATTACAT ACACTGAAGC GCAGAGACAT TCTCAGAATT TAGAAAATGT TGACCAGTCT 
GCATCTAACA AGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCATCC 
TCTTCTGGAT ACCTTGTATC CCCAATGGAA AGCAGTCTTA AGAATTTAGA AAGTGTTGGT 
CAGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGAAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCT CAGCCTCTGC ATCATCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCCA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGTCAGT CTGCTTCTAA CAAGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCTGGAGTCT CCATCCCAGC CTCTGCATCG 
TCCTCTTCTG GATACCTTGC ATCCCTAATG GAAAGCAGTG TTAAGGATAA AGAGCAAACT 
AAAGACACTA TTACATACAC TGAAGCGCAG AGAAATTCTC AGGATAAAGA ACAAACTAAA 
GAAACTATTA CATACAGGGA AAGCCAGATA AATACTCAGA ATTTAGAAAA TGTTGGCCGG 
TCTGCCTCTA ACAAGCAAAA TGGGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT GTCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCAGGAGGC TCCATCTCAG CCTCTGCATC ATCTGCATTG 
TCCTCTTCTG GATACCTTGC ATCCTCAATG GAAAGCAGTG TTAAGGATAA AGAGCAAACC 
AAAGACACTT TTACATACAT TGAAGCTCAG ATAAACTCTC AGGGCAAAGA GCAAACTAAA 
GATATTATTA CATACAGTGA AGCTCAGAGA CATTTTCAGG ATAAAGAGCA ACCTAAAGAG 
AGTATTACAT GTAGTGAAGT TTATAGAAGT ACTCAGGATA AAGAGCAAAC TAAAGACACA 
ATTACATACA GTAAAGTTCA GAGAAATATG CAGAATCTAG AAAATGTTGG TCGGTCTGCC 
CCTAACAGGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTAGGAAA CACTGGACCA 
AGTCTGTATG GGGCTTCTTT AGCAGGATTA TCCGAAGCTC CAAGCTTTCC ATCATCCATC 
TCTTCCCCTC CTGTATACCC TGTGTCTCCA ACAGAAAACA GTCAACGGGT AACCTCAGCA 
GCAATTGATA AAAAGCAAAC TAAAGACTCT ATTACCTACA GTGTTGGTCA GATGAATACT 
CAGGATAAAA AGCAAACTCT ATTAACCTAC AGTGTTGGTC AGATAAAGTC TCAGGATATA 
AAGCAAACTA AAGACACTGT TACATATAGA GAAGGCCACA TAAACGCACA GGATGAACAG 
CAAATGAAAG ACTCCATTAC ATACAGCGCA GCCAAGATAA GTACTCAGAA TCTAGAAAAT 
GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT GTGATGAGCA GTGTATCTGG GGATCGTCTT 
GGATACTTTG GAGCAAGTCT GTATGGGACC TCTTCACATC TGCCCTGGCT TGCTGGATTA 
ATTGAAGCTC CAAGTCTATG TAACACTTGC TCCAGAGTCT CCATCCCAAT CTCAGCATTA 
TGTGCCTGTG CCTCTTCTGG ATCCCATATG TCCCATTGGG ATAGCAGTCA TAAGGAGAAC 
AATCAAACTA AAGACATGAT TACATACAGT GAAGTTCAGA CAAGTATGCA GGATGAACAA 
CAAGCTAAAG GCACTATTAC ATACAGTGAT GGTCAGGTAA AAACTCATGT GTTTGCAGAA 
CAAAAGTCCA ATCACCAAGG CACCAGGAGT ATCAGACAAG AAGGTGGTCA GATGTCAGCG 
CTGATCAAGA ATAGCGAAAT ATCTGAAGAC AATATTACAG ATGATATAAG ATCTCTGTTA 
AGGCCTCATC CTGAGTGTCC AGTTCAGTTT TCGGGCCCAG CCCTTATAAA GGATGTTAGT 
GAGCTGGAGA GAGTGCAGTG CCATTCAACT AAACTGGTAA AAGAGATGGG AGAATTTAGC 
TATGAGGAAA GACAATCAAG TGCAAGCTTT CCCCTGCAGC TCTCATTCAA ACAGGGAGTT 
TCCAAAGACT TGGAGGACAT CACTTCAACC TATGTCTATA GCCCTGGGAT TCATACTGCA 
TGCATAGGCA ATAACAATGT TCTGAAGTCT GTGTATACGT ATAAAGGTTT GCAAACTGAC 
AGCCTTCATG CTGATATCAT GAATGGTAAT GAGTGCAAAT GCACAGAAGA GACCAGCAAT 
CATCTTAATG ACGGCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891109.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746969.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X4, mRNA.

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CloneID	OXa64378
Clone ID Related Accession (Same CDS sequence)	XM_031891109.1
Accession Version	XM_031891109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TGTTTGCAGA ACAAAAGTCC 
AATCACCAAG GCACCAGGAG TATCAGACAA GAAGGTGGTC AGATGTCAGC GCTGATCAAG 
AATAGCGAAA TATCTGAAGA CAATATTACA GATGATATAA GATCTCTGTT AAGGCCTCAT 
CCTGAGTGTC CAGTTCAGTT TTCGGGCCCA GCCCTTATAA AGGATGTTAG TGAGCTGGAG 
AGAGTGCAGT GCCATTCAAC TAAACTGGTA AAAGAGATGG GAGAATTTAG CTATGAGGAA 
AGACAATCAA GTGCAAGCTT TCCCCTGCAG CTCTCATTCA AACAGGGAGT TTCCAAAGAC 
TTGGAGGACA TCACTTCAAC CTATGTCTAT AGCCCTGGGA TTCATACTGC ATGCATAGGC 
AATAACAATG TTCTGAAGTC TGTGTATACG TATAAAGGTT TGCAAACTGA CAGCCTTCAT 
GCTGATATCA TGAATGGTAA TGAGTGCAAA TGCACAGAAG AGACCAGCAA TCATCTTAAT 
GACGGCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746969.1
CDS	441..10172
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891109.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891109.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TGTTTGCAGA ACAAAAGTCC 
AATCACCAAG GCACCAGGAG TATCAGACAA GAAGGTGGTC AGATGTCAGC GCTGATCAAG 
AATAGCGAAA TATCTGAAGA CAATATTACA GATGATATAA GATCTCTGTT AAGGCCTCAT 
CCTGAGTGTC CAGTTCAGTT TTCGGGCCCA GCCCTTATAA AGGATGTTAG TGAGCTGGAG 
AGAGTGCAGT GCCATTCAAC TAAACTGGTA AAAGAGATGG GAGAATTTAG CTATGAGGAA 
AGACAATCAA GTGCAAGCTT TCCCCTGCAG CTCTCATTCA AACAGGGAGT TTCCAAAGAC 
TTGGAGGACA TCACTTCAAC CTATGTCTAT AGCCCTGGGA TTCATACTGC ATGCATAGGC 
AATAACAATG TTCTGAAGTC TGTGTATACG TATAAAGGTT TGCAAACTGA CAGCCTTCAT 
GCTGATATCA TGAATGGTAA TGAGTGCAAA TGCACAGAAG AGACCAGCAA TCATCTTAAT 
GACGGCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891110.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746970.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X5, mRNA.

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CloneID	OXa64379
Clone ID Related Accession (Same CDS sequence)	XM_031891110.1
Accession Version	XM_031891110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGATAAAAAG 
CAAACTAAAG ACTCTATTAC CTACAGTGTT GGTCAGATGA ATACTCAGGA TAAAAAGCAA 
ACTCTATTAA CCTACAGTGT TGGTCAGATA AAGTCTCAGG ATATAAAGCA AACTAAAGAC 
ACTGTTACAT ATAGAGAAGG CCACATAAAC GCACAGGATG AACAGCAAAT GAAAGACTCC 
ATTACATACA GCGCAGCCAA GATAAGTACT CAGAATCTAG AAAATGTTGG TCGGTCTGCC 
CCTAACAGGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC GTCTTGGATA CTTTGGAGCA 
AGTCTGTATG GGACCTCTTC ACATCTGCCC TGGCTTGCTG GATTAATTGA AGCTCCAAGT 
CTATGTAACA CTTGCTCCAG AGTCTCCATC CCAATCTCAG CATTATGTGC CTGTGCCTCT 
TCTGGATCCC ATATGTCCCA TTGGGATAGC AGTCATAAGG AGAACAATCA AACTAAAGAC 
ATGATTACAT ACAGTGAAGT TCAGACAAGT ATGCAGGATG AACAACAAGC TAAAGGCACT 
ATTACATACA GTGATGGTCA GGTAAAAACT CATGTGTTTG CAGAACAAAA GTCCAATCAC 
CAAGGCACCA GGAGTATCAG ACAAGAAGGT GGTCAGATGT CAGCGCTGAT CAAGAATAGC 
GAAATATCTG AAGACAATAT TACAGATGAT ATAAGATCTC TGTTAAGGCC TCATCCTGAG 
TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT ATAAAGGATG TTAGTGAGCT GGAGAGAGTG 
CAGTGCCATT CAACTAAACT GGTAAAAGAG ATGGGAGAAT TTAGCTATGA GGAAAGACAA 
TCAAGTGCAA GCTTTCCCCT GCAGCTCTCA TTCAAACAGG GAGTTTCCAA AGACTTGGAG 
GACATCACTT CAACCTATGT CTATAGCCCT GGGATTCATA CTGCATGCAT AGGCAATAAC 
AATGTTCTGA AGTCTGTGTA TACGTATAAA GGTTTGCAAA CTGACAGCCT TCATGCTGAT 
ATCATGAATG GTAATGAGTG CAAATGCACA GAAGAGACCA GCAATCATCT TAATGACGGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746970.1
CDS	441..10166
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891110.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891110.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGATAAAAAG 
CAAACTAAAG ACTCTATTAC CTACAGTGTT GGTCAGATGA ATACTCAGGA TAAAAAGCAA 
ACTCTATTAA CCTACAGTGT TGGTCAGATA AAGTCTCAGG ATATAAAGCA AACTAAAGAC 
ACTGTTACAT ATAGAGAAGG CCACATAAAC GCACAGGATG AACAGCAAAT GAAAGACTCC 
ATTACATACA GCGCAGCCAA GATAAGTACT CAGAATCTAG AAAATGTTGG TCGGTCTGCC 
CCTAACAGGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC GTCTTGGATA CTTTGGAGCA 
AGTCTGTATG GGACCTCTTC ACATCTGCCC TGGCTTGCTG GATTAATTGA AGCTCCAAGT 
CTATGTAACA CTTGCTCCAG AGTCTCCATC CCAATCTCAG CATTATGTGC CTGTGCCTCT 
TCTGGATCCC ATATGTCCCA TTGGGATAGC AGTCATAAGG AGAACAATCA AACTAAAGAC 
ATGATTACAT ACAGTGAAGT TCAGACAAGT ATGCAGGATG AACAACAAGC TAAAGGCACT 
ATTACATACA GTGATGGTCA GGTAAAAACT CATGTGTTTG CAGAACAAAA GTCCAATCAC 
CAAGGCACCA GGAGTATCAG ACAAGAAGGT GGTCAGATGT CAGCGCTGAT CAAGAATAGC 
GAAATATCTG AAGACAATAT TACAGATGAT ATAAGATCTC TGTTAAGGCC TCATCCTGAG 
TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT ATAAAGGATG TTAGTGAGCT GGAGAGAGTG 
CAGTGCCATT CAACTAAACT GGTAAAAGAG ATGGGAGAAT TTAGCTATGA GGAAAGACAA 
TCAAGTGCAA GCTTTCCCCT GCAGCTCTCA TTCAAACAGG GAGTTTCCAA AGACTTGGAG 
GACATCACTT CAACCTATGT CTATAGCCCT GGGATTCATA CTGCATGCAT AGGCAATAAC 
AATGTTCTGA AGTCTGTGTA TACGTATAAA GGTTTGCAAA CTGACAGCCT TCATGCTGAT 
ATCATGAATG GTAATGAGTG CAAATGCACA GAAGAGACCA GCAATCATCT TAATGACGGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891111.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746971.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X6, mRNA.

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CloneID	OXa64380
Clone ID Related Accession (Same CDS sequence)	XM_031891111.1
Accession Version	XM_031891111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ATACAGCCGG AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ACACGACGAA AGATACATAA AGAGATAGAA 
CCAAATATAC AAACAGAGAT AGAAAAAGAA ATGCAACCAG ACATACGACC AGAAATACAT 
ACAGAGATAG AAAAAGATAT TCAACCAGAG ATACAATCAG AAACACCGAG AGAGGCACAA 
ATAAAAGGAC CGAAAAAAGA TTCTCGTATA CTGACAGTAA TAATTGCAGT TTCGATACTG 
TGCGGAATAT TTGTTGGTAT AGCTCTTTAT TATGGAATAA GACGACGACT AAAGGTTAAA 
AATCAGTCTA ATTGCACTAT TACATACAGT GAAGTTCTGA CAAGTACTCA GGATAAAGAG 
CATATTAAAG ACTCTATTAC ATACAGTGAA GTTCAGATAA ATACTCAGGA TAAAGTGCAT 
ACTAAAGACA CTATTACATA CAGTGACGTT CAGATAAATT CTAGGAGAAA TATGCAGGGC 
AAAGAAGAAC CTGATTATAA TGATTATACT ATTACATTCA GTGAAGGTCA GAGACATACA 
CAGAATAAAA AACAGTCTGA TGATGCTATT ACATACAGTA AAGTTCAGAT AAATACTCAG 
GATAAAGAGC ATATTAAAGA CTCTATTACT TACAGTGACG TTCAGATAAA TACTCAGGAT 
AAAGTGCATA CTAAAGACAC TATTACATAC AGTGACGTTC TGATGAATTC TAGGAGAAAT 
ATGCAGGATA AAGAGCAAAC TAAAGGCACT ATTACATACA CTGAAGCGCA GAGACATTCT 
CAGAATTTAG AAAATGTTGA CCAGTCTGCA TCTAACAAGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTTGGATA CTCTGGGTCA GGTCTATATG GCGCCTCTTT ACATCAGACC 
AGATCTGCAG GATTATCCGA AGCTACAACT CTATACAAAA ATGTCTCTGG AGTCTCCATC 
TCAGCCTCTG CATCATCTGC ATCATCCTCT TCTGGATACC TTGTATCCCC AATGGAAAGC 
AGTCTTAAGA ATTTAGAAAG TGTTGGTCAG TCTGCCTCTA ACAAGCAAAA TGGGATGAGC 
AGTGAATCTG GGGATCATCT TGGATACTCT GGGTCAGGTC TATATGGCGC CTCTTTACAT 
CAGACCAGAT CTGCAGGATT ATCCGAAGCT ACAACTCTAT ACAAAAATGT CTCTGGAGTC 
TCCATCTCAG CCTCTGCATC ATCTGCATCG TCCTCTTCTG GATACCTTGC ATCCCCAATG 
GAAAGCAGTG TTAAGAATTT AGAAAATGTT GGTCAGTCTG CTTCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCCCAGCCTC TGCATCGTCC TCTTCTGGAT ACCTTGCATC CCTAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACTAAA GACACTATTA CATACACTGA AGCGCAGAGA 
AATTCTCAGG ATAAAGAACA AACTAAAGAA ACTATTACAT ACAGGGAAAG CCAGATAAAT 
ACTCAGAATT TAGAAAATGT TGGCCGGTCT GCCTCTAACA AGCAAAATGG GATGAGCAGT 
GTATCTGGGG ATCATCTTGG ATACTCTGGG TCAGGTCTAT ATGGCGCCTC TTTACATCAG 
ACCAGATCTG CAGGATTGTC CGAAGCTACA ACTCTATACA AAAATGTCTC AGGAGGCTCC 
ATCTCAGCCT CTGCATCATC TGCATTGTCC TCTTCTGGAT ACCTTGCATC CTCAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACCAAA GACACTTTTA CATACATTGA AGCTCAGATA 
AACTCTCAGG GCAAAGAGCA AACTAAAGAT ATTATTACAT ACAGTGAAGC TCAGAGACAT 
TTTCAGGATA AAGAGCAACC TAAAGAGAGT ATTACATGTA GTGAAGTTTA TAGAAGTACT 
CAGGATAAAG AGCAAACTAA AGACACAATT ACATACAGTA AAGTTCAGAG AAATATGCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCATC TAGGAAACAC TGGACCAAGT CTGTATGGGG CTTCTTTAGC AGGATTATCC 
GAAGCTCCAA GCTTTCCATC ATCCATCTCT TCCCCTCCTG TATACCCTGT GTCTCCAACA 
GAAAACAGTC AACGGGTAAC CTCAGCAGCA ATTGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCACAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGCGCAGCC 
AAGATAAGTA CTCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACCTCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGGAGTATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCT CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC CAGCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746971.1
CDS	441..10148
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891111.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891111.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ATACAGCCGG AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ACACGACGAA AGATACATAA AGAGATAGAA 
CCAAATATAC AAACAGAGAT AGAAAAAGAA ATGCAACCAG ACATACGACC AGAAATACAT 
ACAGAGATAG AAAAAGATAT TCAACCAGAG ATACAATCAG AAACACCGAG AGAGGCACAA 
ATAAAAGGAC CGAAAAAAGA TTCTCGTATA CTGACAGTAA TAATTGCAGT TTCGATACTG 
TGCGGAATAT TTGTTGGTAT AGCTCTTTAT TATGGAATAA GACGACGACT AAAGGTTAAA 
AATCAGTCTA ATTGCACTAT TACATACAGT GAAGTTCTGA CAAGTACTCA GGATAAAGAG 
CATATTAAAG ACTCTATTAC ATACAGTGAA GTTCAGATAA ATACTCAGGA TAAAGTGCAT 
ACTAAAGACA CTATTACATA CAGTGACGTT CAGATAAATT CTAGGAGAAA TATGCAGGGC 
AAAGAAGAAC CTGATTATAA TGATTATACT ATTACATTCA GTGAAGGTCA GAGACATACA 
CAGAATAAAA AACAGTCTGA TGATGCTATT ACATACAGTA AAGTTCAGAT AAATACTCAG 
GATAAAGAGC ATATTAAAGA CTCTATTACT TACAGTGACG TTCAGATAAA TACTCAGGAT 
AAAGTGCATA CTAAAGACAC TATTACATAC AGTGACGTTC TGATGAATTC TAGGAGAAAT 
ATGCAGGATA AAGAGCAAAC TAAAGGCACT ATTACATACA CTGAAGCGCA GAGACATTCT 
CAGAATTTAG AAAATGTTGA CCAGTCTGCA TCTAACAAGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTTGGATA CTCTGGGTCA GGTCTATATG GCGCCTCTTT ACATCAGACC 
AGATCTGCAG GATTATCCGA AGCTACAACT CTATACAAAA ATGTCTCTGG AGTCTCCATC 
TCAGCCTCTG CATCATCTGC ATCATCCTCT TCTGGATACC TTGTATCCCC AATGGAAAGC 
AGTCTTAAGA ATTTAGAAAG TGTTGGTCAG TCTGCCTCTA ACAAGCAAAA TGGGATGAGC 
AGTGAATCTG GGGATCATCT TGGATACTCT GGGTCAGGTC TATATGGCGC CTCTTTACAT 
CAGACCAGAT CTGCAGGATT ATCCGAAGCT ACAACTCTAT ACAAAAATGT CTCTGGAGTC 
TCCATCTCAG CCTCTGCATC ATCTGCATCG TCCTCTTCTG GATACCTTGC ATCCCCAATG 
GAAAGCAGTG TTAAGAATTT AGAAAATGTT GGTCAGTCTG CTTCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCCCAGCCTC TGCATCGTCC TCTTCTGGAT ACCTTGCATC CCTAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACTAAA GACACTATTA CATACACTGA AGCGCAGAGA 
AATTCTCAGG ATAAAGAACA AACTAAAGAA ACTATTACAT ACAGGGAAAG CCAGATAAAT 
ACTCAGAATT TAGAAAATGT TGGCCGGTCT GCCTCTAACA AGCAAAATGG GATGAGCAGT 
GTATCTGGGG ATCATCTTGG ATACTCTGGG TCAGGTCTAT ATGGCGCCTC TTTACATCAG 
ACCAGATCTG CAGGATTGTC CGAAGCTACA ACTCTATACA AAAATGTCTC AGGAGGCTCC 
ATCTCAGCCT CTGCATCATC TGCATTGTCC TCTTCTGGAT ACCTTGCATC CTCAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACCAAA GACACTTTTA CATACATTGA AGCTCAGATA 
AACTCTCAGG GCAAAGAGCA AACTAAAGAT ATTATTACAT ACAGTGAAGC TCAGAGACAT 
TTTCAGGATA AAGAGCAACC TAAAGAGAGT ATTACATGTA GTGAAGTTTA TAGAAGTACT 
CAGGATAAAG AGCAAACTAA AGACACAATT ACATACAGTA AAGTTCAGAG AAATATGCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCATC TAGGAAACAC TGGACCAAGT CTGTATGGGG CTTCTTTAGC AGGATTATCC 
GAAGCTCCAA GCTTTCCATC ATCCATCTCT TCCCCTCCTG TATACCCTGT GTCTCCAACA 
GAAAACAGTC AACGGGTAAC CTCAGCAGCA ATTGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCACAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGCGCAGCC 
AAGATAAGTA CTCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACCTCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGGAGTATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCT CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC CAGCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891112.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746972.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X7, mRNA.

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CloneID	OXa64381
Clone ID Related Accession (Same CDS sequence)	XM_031891112.1
Accession Version	XM_031891112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAACAA 
ACTAAAGAAA CTATTACATA CAGGGAAAGC CAGATAAATA CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746972.1
CDS	441..10127
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891112.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891112.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAACAA 
ACTAAAGAAA CTATTACATA CAGGGAAAGC CAGATAAATA CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746973.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X8, mRNA.

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CloneID	OXa64382
Clone ID Related Accession (Same CDS sequence)	XM_031891113.1
Accession Version	XM_031891113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746973.1
CDS	441..10127
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891113.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891114.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746974.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X9, mRNA.

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CloneID	OXa64383
Clone ID Related Accession (Same CDS sequence)	XM_031891114.1
Accession Version	XM_031891114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746974.1
CDS	441..10127
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891114.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891114.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891116.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746976.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X10, mRNA.

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CloneID	OXa64384
Clone ID Related Accession (Same CDS sequence)	XM_031891116.1
Accession Version	XM_031891116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGACGTTCT GATGAATTCT AGGAGAAATA TGCAGGATAA AGAGCAAACT 
AAAGGCACTA TTACATACAC TGAAGCGCAG AGACATTCTC AGAATTTAGA AAATGTTGAC 
CAGTCTGCAT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCT CAGCCTCTGC ATCATCTGCA 
TCATCCTCTT CTGGATACCT TGTATCCCCA ATGGAAAGCA GTCTTAAGAA TTTAGAAAGT 
GTTGGTCAGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGAATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCTGGAGTCT CCATCTCAGC CTCTGCATCA 
TCTGCATCGT CCTCTTCTGG ATACCTTGCA TCCCCAATGG AAAGCAGTGT TAAGAATTTA 
GAAAATGTTG GTCAGTCTGC TTCTAACAAG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CATCTTGGAT ACTCTGGGTC AGGTCTATAT GGCGCCTCTT TACATCAGAC CAGATCTGCA 
GGATTATCCG AAGCTACAAC TCTATACAAA AATGTCTCTG GAGTCTCCAT CCCAGCCTCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CTAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACTAAAG ACACTATTAC ATACACTGAA GCGCAGAGAA ATTCTCAGGA TAAAGAACAA 
ACTAAAGAAA CTATTACATA CAGGGAAAGC CAGATAAATA CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746976.1
CDS	441..10127
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891116.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891116.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGACGTTCT GATGAATTCT AGGAGAAATA TGCAGGATAA AGAGCAAACT 
AAAGGCACTA TTACATACAC TGAAGCGCAG AGACATTCTC AGAATTTAGA AAATGTTGAC 
CAGTCTGCAT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCT CAGCCTCTGC ATCATCTGCA 
TCATCCTCTT CTGGATACCT TGTATCCCCA ATGGAAAGCA GTCTTAAGAA TTTAGAAAGT 
GTTGGTCAGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGAATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTA 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCTGGAGTCT CCATCTCAGC CTCTGCATCA 
TCTGCATCGT CCTCTTCTGG ATACCTTGCA TCCCCAATGG AAAGCAGTGT TAAGAATTTA 
GAAAATGTTG GTCAGTCTGC TTCTAACAAG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CATCTTGGAT ACTCTGGGTC AGGTCTATAT GGCGCCTCTT TACATCAGAC CAGATCTGCA 
GGATTATCCG AAGCTACAAC TCTATACAAA AATGTCTCTG GAGTCTCCAT CCCAGCCTCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CTAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACTAAAG ACACTATTAC ATACACTGAA GCGCAGAGAA ATTCTCAGGA TAAAGAACAA 
ACTAAAGAAA CTATTACATA CAGGGAAAGC CAGATAAATA CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891117.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746977.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X11, mRNA.

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CloneID	OXa64385
Clone ID Related Accession (Same CDS sequence)	XM_031891117.1
Accession Version	XM_031891117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746977.1
CDS	441..10127
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891117.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891117.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891118.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746978.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X12, mRNA.

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CloneID	OXa64386
Clone ID Related Accession (Same CDS sequence)	XM_031891118.1
Accession Version	XM_031891118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGAACAA AGATACAAAC AGAGCTACGA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAACAG AGCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACGGAG ATACAACCAA AGATACAAAC AGAGATACAA 
TCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACTA 
CCGGAGTTAC GAACAACGAT AAAAACAGAC ACAAAATCAG AAATACAAAC AGACATAAAG 
ATACAAACTG AGGAAAAAAT AAAAGCAGGA GGAGAAATCC AAGTTATTGG GCGTTTAGGG 
GAGACTGTCA CACTGCAGTG CCCTCTGAAT TCACCAGCAA AAATCTCATG GATGTTTGAT 
AATAATGGGG AAAAAATTCT CCTGGCTGAA TACAGAGACC AGAAGTTCAT CAGAGTGAAT 
AACAACTACT TCATCAACCG GCTGGAAGGG TCTAACAGTG GCACCGCATT AAGGATCAAA 
CATCTGAGAA TGGAGGATGG TGGGGTCTTC ACTGCTCACA TCACTGTTAA TGGAAGCGCT 
ACAGAAATTA TTTATGTTCT CACAGTGTTG GGAAGAGGAG ACATCATTCT GCCAGTGACT 
GGCCTTGTAG GGGGGAATGT CACACTGCCA ACCTATCTGA CTCTGCCAGC TGCCATCGAA 
AGAATCTCAT GGATGTTTCA TGACAAGGGG AAGCGAATAC ACCTGGCTGA ATTCAGACAC 
CAGAAGTTTA TCACAGTTAA TAAGAAATAT TTCTTAAACC GCCTTGAAAA ACCTAATGAT 
GGAACCGCAT TAAGGATCAG AGAGCTGAGA ATGGAGGATT CTGGGATCTT CACTGCACAC 
ATTCATTTTA ATGGACGAAA AAGAGACATT TCTTACATTC TAGCCGTTTT CGAGCCTATC 
CCAACACCAG TGATAAAAGT GACGTTTGAT GAGAATTCCC CTGATCTCTG CCATCTTCAT 
TGTTCTGTCC CATCAAACTC ACCAACCCTG TCCTACAGTT GGACCTACAG AGATACTGGC 
AATGACAGAC TATATGCCAA TGGCAGCACA ATCACTGTAT CACTGAAGGA TAAGGCATTG 
GGGGCAGAAT TTACCTGCTG GGTACAGAAT CCTGCTCACA GGAACAATGC ATCAGTCCCT 
TTAAAGTCAC TTCATGGGAG ACGAACAGGA ATACAAACTG AGATACAACC ATGGATACAA 
AGAAAGATAC AATCAAAGAT ACAAAAAGAG ATACGACCAG ATATAAAAGC AGAGATTCAA 
CATAAAATAC AAACAGACAC AAAACTAGGA ACGCAGACAG AGATACAACC TGGAACAGAG 
ATACATCCAG AAATACAACC AGAGATACAA ATAGAGCTAC GACCCAGGAT ACAAACAGAG 
ATACAACCAG ATATAAAAGC AGAGATACGA CCAAAGATAC AAGCAGGGAT ACACACTGAG 
CTACAACCAA AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAC ACAAACAGAG 
ATACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTGCGACC AGGGATACAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTTCGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACAACA AGGGATACAA ACAGAGCTAC GTCCAGAGAT AAAAACTGAG 
CTAGTACCAG GGATACGTAC AGATATACAA CCAGGGATAC AAACAGATCT ACGAACAGAG 
ATACAAAAAA AGATACAAAC AGACACCAAA CCAGAAATAC TAACAGAGGT GCAAACAGAC 
ATAAAGAGAC AAACAAAGGC AAAAATAAAA GCAGGAGGAG ACATCCAGGT AATTGGCCGT 
GTAGGGGAGA CTGTCACACT GCAGTGCCCT CTGAACCTAC CAACACGAAT CTTGTGGACG 
TATGATGATA ATGGGGAAAA AATACTCCTG GCTGAATACA GAGACCAGAA ATTTATCAGA 
GTGAATAACA ACTACTTCAT CAACCGGCTG GAAGGGTCTA ACAGTGGCAC CGCATTACTG 
ATCAGAGAGC TAAAAAGAGA GGATAGTGGG GTCTTCACTG CTCACATTAC TGTTAATGGA 
AGCGCTACAG ACATTAGTTA TATACTGAAT GTACTGGGAA GAGGAGACAT CATTCTGCCA 
GTGACTGGCC TTGTAGGGGG GAATGTCACA CTGCCAACCT ATCTGACTCT GCCAGCTGCC 
ATCGAAAGAA TCTCATTGAT GTTTCATGAC AAAGGCAAAA AAATACACCT GGCTGAATTA 
AGAAACCATA AGTTTAGCAG ACTGAACAAG GATTATTTCT ACAACCGCCT TGAAAAATCT 
AATGATGGAA CCGCATTACA GATCAGGGAG CTGAGAATGG AGGATTCTGG GATCTTCACT 
GCACACATTC ATGTTAATGG ACAAAAAAGA GACATTTCTT ATATTCTAGC CGTGTTCGAG 
CCTATCCCAA CACCAGTAAT AGAAGTGACA TCTGATGAGA ATTTCCCTGA TCTCTGCCAT 
CTTCATTGTT CTGTCCCATC AAACTCACCA ACCCTGTCCT ACAGTTGGAC CTACAGAGAT 
ACTGGCACTG ACAGACTATA TGCCAATGGC AGCACAATCA CTGTATCACT GAAGGATAAG 
GCATTGGGGG CAGAATTTAC CTGCTGGGTA CAGAATCCTG CTCACAGGAA CAGTGTGTCA 
GTCGTTTTAA AGTCATATAG TGGGAGACAA ACAGGAATAC AACCAAAGAT ACGACCAGAG 
ATACAACAAA AAATACAAAC GGAGACACAA CCAGAGATAA AAACAGAGAT ACGACCAGGG 
ATAAAAACAG AGATACAACC AGAGATAAAA ACAGAAATAC AGCCAGGGAT AAAAACAGAG 
AAACAACCAG GGATACTAAC GGAGATACGA CCAGGGATAA AAACAGAGAT GCGACCAGGG 
GTGCAAAAAG AGATACAACT AGGAATACAA AAAGAAATAC GATCAGAGAT ACAAACTGAG 
ATACGACAAG GGATACAAAG AGAGACACAA AAAGAGACAG AAATAAAAGC AGGAGGAGAC 
ATCCAGGTGA TTGGCCGTGT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATCTACCA 
ACAAGAATCT TGTGGACGTA TGATGATAAT GGGGAAAAAA TACTCCTGGC TGAATACAGA 
GACCAGAAGT TTATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGATCTAAC 
AGTGGCACTG CATTAAGGAT CAAAAACCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTAGTTATA TTGTCAATGT ATTTGGAAGA 
GGAGACATCA TTGTCCAGGC AACTGGGCGT GAAGGGGACA CTGTCACACT GCCAACCTAT 
ATGAATCACG CAACTTCCAT TGAAAGAATC TCATGGATGT TTCATGACAA AGGGAAGAAA 
ATACACCTGG CTGAATTCAG AAACCACAAG TTTATCAGAG TAAATAAGAA TTATTTCCCC 
AACCGTCTTG AAAAATCGAA TTATGGAACC GCATTGCAGA TCAAAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTTAT GCTAATGGAA GAAAGAGAGA CATTTCTTAT 
ATTCTTGTTG TGTTCGAGCT TATACCAACA CCAGTAATAG AAGTGACGTA TGACGAGAAT 
TTTACTGATC TCTGCCATCT TCATTGTTCT GTCAATTCAA ACTCAACAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGACAA TGGCACTGAC ATACTATATG CCAATGGCAG CACAATCAGT 
ATATTACTAA AGAATATGGC ATCAGGCTCA GAATTTACCT GCTTGGTACA AAATCCTGTT 
CACAGGAACA GTGCGTCAGT CCTTTTAAAG TCACTTGGTG GAAGACGAAC AGATATACGA 
AAAGAGATAC AAACTGAGAT ACGACAAGGA ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
ACAGGGATAC AAAGAGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACGG AGATACAAAC AGAGATACGA 
CAAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACGG AGATACAAAC AGAGATACGA 
CAAGGGATAC AGAGAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCAAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAGAG AGAGATACAG 
CCAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGATACAGCC GGAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGACACGACG AAAGATACAT 
AAAGAGATAG AACCAAATAT ACAAACAGAG ATAGAAAAAG AAATGCAACC AGACATACGA 
CCAGAAATAC ATACAGAGAT AGAAAAAGAT ATTCAACCAG AGATACAATC AGAAACACCG 
AGAGAGGCAC AAATAAAAGG ACCGAAAAAA GATTCTCGTA TACTGACAGT AATAATTGCA 
GTTTCGATAC TGTGCGGAAT ATTTGTTGGT ATAGCTCTTT ATTATGGAAT AAGACGACGA 
CTAAAGGTTA AAAATCAGTC TAATTGCACT ATTACATACA GTGAAGTTCT GACAAGTACT 
CAGGATAAAG AGCATATTAA AGACTCTATT ACATACAGTG AAGTTCAGAT AAATACTCAG 
GATAAAGTGC ATACTAAAGA CACTATTACA TACAGTGACG TTCAGATAAA TTCTAGGAGA 
AATATGCAGG GCAAAGAAGA ACCTGATTAT AATGATTATA CTATTACATT CAGTGAAGGT 
CAGAGACATA CACAGAATAA AAAACAGTCT GATGATGCTA TTACATACAG TAAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA CGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGACGT TCTGATGAAT 
TCTAGGAGAA ATATGCAGGA TAAAGAGCAA ACTAAAGGCA CTATTACATA CACTGAAGCG 
CAGAGACATT CTCAGAATTT AGAAAATGTT GACCAGTCTG CATCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCTCAGCCTC TGCATCATCT GCATCATCCT CTTCTGGATA CCTTGTATCC 
CCAATGGAAA GCAGTCTTAA GAATTTAGAA AGTGTTGGTC AGTCTGCCTC TAACAAGCAA 
AATGGGATGA GCAGTGAATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCTGGAG TCTCCATCTC AGCCTCTGCA TCATCTGCAT CGTCCTCTTC TGGATACCTT 
GCATCCCCAA TGGAAAGCAG TGTTAAGAAT TTAGAAAATG TTGGTCAGTC TGCTTCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCCCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCTAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCGCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG GGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TCTTTACATC AGACCAGATC TGCAGGATTG TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCTGCATTGT CCTCTTCTGG ATACCTTGCA 
TCCTCAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACCA AAGACACTTT TACATACATT 
GAAGCTCAGA TAAACTCTCA GGGCAAAGAG CAAACTAAAG ATATTATTAC ATACAGTGAA 
GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA CCTAAAGAGA GTATTACATG TAGTGAAGTT 
TATAGAAGTA CTCAGGATAA AGAGCAAACT AAAGACACAA TTACATACAG TAAAGTTCAG 
AGAAATATGC AGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCA TCTAGGAAAC ACTGGACCAA GTCTGTATGG GGCTTCTTTA 
GCAGGATTAT CCGAAGCTCC AAGCTTTCCA TCATCCATCT CTTCCCCTCC TGTATACCCT 
GTGTCTCCAA CAGAAAACAG TCAACGGGTA ACCTCAGCAG CAATTGATAA AAAGCAAACT 
AAAGACTCTA TTACCTACAG TGTTGGTCAG ATGAATACTC AGGATAAAAA GCAAACTCTA 
TTAACCTACA GTGTTGGTCA GATAAAGTCT CAGGATATAA AGCAAACTAA AGACACTGTT 
ACATATAGAG AAGGCCACAT AAACGCACAG GATGAACAGC AAATGAAAGA CTCCATTACA 
TACAGCGCAG CCAAGATAAG TACTCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCGTCTTG GATACTTTGG AGCAAGTCTG 
TATGGGACCT CTTCACATCT GCCCTGGCTT GCTGGATTAA TTGAAGCTCC AAGTCTATGT 
AACACTTGCT CCAGAGTCTC CATCCCAATC TCAGCATTAT GTGCCTGTGC CTCTTCTGGA 
TCCCATATGT CCCATTGGGA TAGCAGTCAT AAGGAGAACA ATCAAACTAA AGACATGATT 
ACATACAGTG AAGTTCAGAC AAGTATGCAG GATGAACAAC AAGCTAAAGG CACTATTACA 
TACAGTGATG GTCAGGTAAA AACTCATGTG TTTGCAGAAC AAAAGTCCAA TCACCAAGGC 
ACCAGGAGTA TCAGACAAGA AGGTGGTCAG ATGTCAGCGC TGATCAAGAA TAGCGAAATA 
TCTGAAGACA ATATTACAGA TGATATAAGA TCTCTGTTAA GGCCTCATCC TGAGTGTCCA 
GTTCAGTTTT CGGGCCCAGC CCTTATAAAG GATGTTAGTG AGCTGGAGAG AGTGCAGTGC 
CATTCAACTA AACTGGTAAA AGAGATGGGA GAATTTAGCT ATGAGGAAAG ACAATCAAGT 
GCAAGCTTTC CCCTGCAGCT CTCATTCAAA CAGGGAGTTT CCAAAGACTT GGAGGACATC 
ACTTCAACCT ATGTCTATAG CCCTGGGATT CATACTGCAT GCATAGGCAA TAACAATGTT 
CTGAAGTCTG TGTATACGTA TAAAGGTTTG CAAACTGACA GCCTTCATGC TGATATCATG 
AATGGTAATG AGTGCAAATG CACAGAAGAG ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746978.1
CDS	441..10100
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891118.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891118.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC GACCAGGAAT ACAAACAGAG ATACGAACAA AGATACAAAC AGAGCTACGA 
CCAGAGATAA AAACTAAGCT AGAACCAGGG ATACAAACAG AGCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACGGAG ATACAACCAA AGATACAAAC AGAGATACAA 
TCAAAGATAC AAACGGAGAT ACAACCAAGG ATACAAACAG AGATACAACC AAAGATACAA 
ACAGAGATAC AACCAAGGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACTA 
CCGGAGTTAC GAACAACGAT AAAAACAGAC ACAAAATCAG AAATACAAAC AGACATAAAG 
ATACAAACTG AGGAAAAAAT AAAAGCAGGA GGAGAAATCC AAGTTATTGG GCGTTTAGGG 
GAGACTGTCA CACTGCAGTG CCCTCTGAAT TCACCAGCAA AAATCTCATG GATGTTTGAT 
AATAATGGGG AAAAAATTCT CCTGGCTGAA TACAGAGACC AGAAGTTCAT CAGAGTGAAT 
AACAACTACT TCATCAACCG GCTGGAAGGG TCTAACAGTG GCACCGCATT AAGGATCAAA 
CATCTGAGAA TGGAGGATGG TGGGGTCTTC ACTGCTCACA TCACTGTTAA TGGAAGCGCT 
ACAGAAATTA TTTATGTTCT CACAGTGTTG GGAAGAGGAG ACATCATTCT GCCAGTGACT 
GGCCTTGTAG GGGGGAATGT CACACTGCCA ACCTATCTGA CTCTGCCAGC TGCCATCGAA 
AGAATCTCAT GGATGTTTCA TGACAAGGGG AAGCGAATAC ACCTGGCTGA ATTCAGACAC 
CAGAAGTTTA TCACAGTTAA TAAGAAATAT TTCTTAAACC GCCTTGAAAA ACCTAATGAT 
GGAACCGCAT TAAGGATCAG AGAGCTGAGA ATGGAGGATT CTGGGATCTT CACTGCACAC 
ATTCATTTTA ATGGACGAAA AAGAGACATT TCTTACATTC TAGCCGTTTT CGAGCCTATC 
CCAACACCAG TGATAAAAGT GACGTTTGAT GAGAATTCCC CTGATCTCTG CCATCTTCAT 
TGTTCTGTCC CATCAAACTC ACCAACCCTG TCCTACAGTT GGACCTACAG AGATACTGGC 
AATGACAGAC TATATGCCAA TGGCAGCACA ATCACTGTAT CACTGAAGGA TAAGGCATTG 
GGGGCAGAAT TTACCTGCTG GGTACAGAAT CCTGCTCACA GGAACAATGC ATCAGTCCCT 
TTAAAGTCAC TTCATGGGAG ACGAACAGGA ATACAAACTG AGATACAACC ATGGATACAA 
AGAAAGATAC AATCAAAGAT ACAAAAAGAG ATACGACCAG ATATAAAAGC AGAGATTCAA 
CATAAAATAC AAACAGACAC AAAACTAGGA ACGCAGACAG AGATACAACC TGGAACAGAG 
ATACATCCAG AAATACAACC AGAGATACAA ATAGAGCTAC GACCCAGGAT ACAAACAGAG 
ATACAACCAG ATATAAAAGC AGAGATACGA CCAAAGATAC AAGCAGGGAT ACACACTGAG 
CTACAACCAA AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGGGATAAAA ACAGAGATAC GACCAGGGAC ACAAACAGAG 
ATACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTGCGACC AGGGATACAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTTCGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACAACA AGGGATACAA ACAGAGCTAC GTCCAGAGAT AAAAACTGAG 
CTAGTACCAG GGATACGTAC AGATATACAA CCAGGGATAC AAACAGATCT ACGAACAGAG 
ATACAAAAAA AGATACAAAC AGACACCAAA CCAGAAATAC TAACAGAGGT GCAAACAGAC 
ATAAAGAGAC AAACAAAGGC AAAAATAAAA GCAGGAGGAG ACATCCAGGT AATTGGCCGT 
GTAGGGGAGA CTGTCACACT GCAGTGCCCT CTGAACCTAC CAACACGAAT CTTGTGGACG 
TATGATGATA ATGGGGAAAA AATACTCCTG GCTGAATACA GAGACCAGAA ATTTATCAGA 
GTGAATAACA ACTACTTCAT CAACCGGCTG GAAGGGTCTA ACAGTGGCAC CGCATTACTG 
ATCAGAGAGC TAAAAAGAGA GGATAGTGGG GTCTTCACTG CTCACATTAC TGTTAATGGA 
AGCGCTACAG ACATTAGTTA TATACTGAAT GTACTGGGAA GAGGAGACAT CATTCTGCCA 
GTGACTGGCC TTGTAGGGGG GAATGTCACA CTGCCAACCT ATCTGACTCT GCCAGCTGCC 
ATCGAAAGAA TCTCATTGAT GTTTCATGAC AAAGGCAAAA AAATACACCT GGCTGAATTA 
AGAAACCATA AGTTTAGCAG ACTGAACAAG GATTATTTCT ACAACCGCCT TGAAAAATCT 
AATGATGGAA CCGCATTACA GATCAGGGAG CTGAGAATGG AGGATTCTGG GATCTTCACT 
GCACACATTC ATGTTAATGG ACAAAAAAGA GACATTTCTT ATATTCTAGC CGTGTTCGAG 
CCTATCCCAA CACCAGTAAT AGAAGTGACA TCTGATGAGA ATTTCCCTGA TCTCTGCCAT 
CTTCATTGTT CTGTCCCATC AAACTCACCA ACCCTGTCCT ACAGTTGGAC CTACAGAGAT 
ACTGGCACTG ACAGACTATA TGCCAATGGC AGCACAATCA CTGTATCACT GAAGGATAAG 
GCATTGGGGG CAGAATTTAC CTGCTGGGTA CAGAATCCTG CTCACAGGAA CAGTGTGTCA 
GTCGTTTTAA AGTCATATAG TGGGAGACAA ACAGGAATAC AACCAAAGAT ACGACCAGAG 
ATACAACAAA AAATACAAAC GGAGACACAA CCAGAGATAA AAACAGAGAT ACGACCAGGG 
ATAAAAACAG AGATACAACC AGAGATAAAA ACAGAAATAC AGCCAGGGAT AAAAACAGAG 
AAACAACCAG GGATACTAAC GGAGATACGA CCAGGGATAA AAACAGAGAT GCGACCAGGG 
GTGCAAAAAG AGATACAACT AGGAATACAA AAAGAAATAC GATCAGAGAT ACAAACTGAG 
ATACGACAAG GGATACAAAG AGAGACACAA AAAGAGACAG AAATAAAAGC AGGAGGAGAC 
ATCCAGGTGA TTGGCCGTGT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATCTACCA 
ACAAGAATCT TGTGGACGTA TGATGATAAT GGGGAAAAAA TACTCCTGGC TGAATACAGA 
GACCAGAAGT TTATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGATCTAAC 
AGTGGCACTG CATTAAGGAT CAAAAACCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTAGTTATA TTGTCAATGT ATTTGGAAGA 
GGAGACATCA TTGTCCAGGC AACTGGGCGT GAAGGGGACA CTGTCACACT GCCAACCTAT 
ATGAATCACG CAACTTCCAT TGAAAGAATC TCATGGATGT TTCATGACAA AGGGAAGAAA 
ATACACCTGG CTGAATTCAG AAACCACAAG TTTATCAGAG TAAATAAGAA TTATTTCCCC 
AACCGTCTTG AAAAATCGAA TTATGGAACC GCATTGCAGA TCAAAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTTAT GCTAATGGAA GAAAGAGAGA CATTTCTTAT 
ATTCTTGTTG TGTTCGAGCT TATACCAACA CCAGTAATAG AAGTGACGTA TGACGAGAAT 
TTTACTGATC TCTGCCATCT TCATTGTTCT GTCAATTCAA ACTCAACAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGACAA TGGCACTGAC ATACTATATG CCAATGGCAG CACAATCAGT 
ATATTACTAA AGAATATGGC ATCAGGCTCA GAATTTACCT GCTTGGTACA AAATCCTGTT 
CACAGGAACA GTGCGTCAGT CCTTTTAAAG TCACTTGGTG GAAGACGAAC AGATATACGA 
AAAGAGATAC AAACTGAGAT ACGACAAGGA ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
ACAGGGATAC AAAGAGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACGG AGATACAAAC AGAGATACGA 
CAAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACGG AGATACAAAC AGAGATACGA 
CAAGGGATAC AGAGAGAGAT ACAGCCGGAG ATACAAACAG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCAAAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAGAG AGAGATACAG 
CCAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGATACAGCC GGAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGACACGACG AAAGATACAT 
AAAGAGATAG AACCAAATAT ACAAACAGAG ATAGAAAAAG AAATGCAACC AGACATACGA 
CCAGAAATAC ATACAGAGAT AGAAAAAGAT ATTCAACCAG AGATACAATC AGAAACACCG 
AGAGAGGCAC AAATAAAAGG ACCGAAAAAA GATTCTCGTA TACTGACAGT AATAATTGCA 
GTTTCGATAC TGTGCGGAAT ATTTGTTGGT ATAGCTCTTT ATTATGGAAT AAGACGACGA 
CTAAAGGTTA AAAATCAGTC TAATTGCACT ATTACATACA GTGAAGTTCT GACAAGTACT 
CAGGATAAAG AGCATATTAA AGACTCTATT ACATACAGTG AAGTTCAGAT AAATACTCAG 
GATAAAGTGC ATACTAAAGA CACTATTACA TACAGTGACG TTCAGATAAA TTCTAGGAGA 
AATATGCAGG GCAAAGAAGA ACCTGATTAT AATGATTATA CTATTACATT CAGTGAAGGT 
CAGAGACATA CACAGAATAA AAAACAGTCT GATGATGCTA TTACATACAG TAAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA CGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGACGT TCTGATGAAT 
TCTAGGAGAA ATATGCAGGA TAAAGAGCAA ACTAAAGGCA CTATTACATA CACTGAAGCG 
CAGAGACATT CTCAGAATTT AGAAAATGTT GACCAGTCTG CATCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCTCAGCCTC TGCATCATCT GCATCATCCT CTTCTGGATA CCTTGTATCC 
CCAATGGAAA GCAGTCTTAA GAATTTAGAA AGTGTTGGTC AGTCTGCCTC TAACAAGCAA 
AATGGGATGA GCAGTGAATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCTGGAG TCTCCATCTC AGCCTCTGCA TCATCTGCAT CGTCCTCTTC TGGATACCTT 
GCATCCCCAA TGGAAAGCAG TGTTAAGAAT TTAGAAAATG TTGGTCAGTC TGCTTCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCCCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCTAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCGCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG GGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TCTTTACATC AGACCAGATC TGCAGGATTG TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCTGCATTGT CCTCTTCTGG ATACCTTGCA 
TCCTCAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACCA AAGACACTTT TACATACATT 
GAAGCTCAGA TAAACTCTCA GGGCAAAGAG CAAACTAAAG ATATTATTAC ATACAGTGAA 
GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA CCTAAAGAGA GTATTACATG TAGTGAAGTT 
TATAGAAGTA CTCAGGATAA AGAGCAAACT AAAGACACAA TTACATACAG TAAAGTTCAG 
AGAAATATGC AGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCA TCTAGGAAAC ACTGGACCAA GTCTGTATGG GGCTTCTTTA 
GCAGGATTAT CCGAAGCTCC AAGCTTTCCA TCATCCATCT CTTCCCCTCC TGTATACCCT 
GTGTCTCCAA CAGAAAACAG TCAACGGGTA ACCTCAGCAG CAATTGATAA AAAGCAAACT 
AAAGACTCTA TTACCTACAG TGTTGGTCAG ATGAATACTC AGGATAAAAA GCAAACTCTA 
TTAACCTACA GTGTTGGTCA GATAAAGTCT CAGGATATAA AGCAAACTAA AGACACTGTT 
ACATATAGAG AAGGCCACAT AAACGCACAG GATGAACAGC AAATGAAAGA CTCCATTACA 
TACAGCGCAG CCAAGATAAG TACTCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCGTCTTG GATACTTTGG AGCAAGTCTG 
TATGGGACCT CTTCACATCT GCCCTGGCTT GCTGGATTAA TTGAAGCTCC AAGTCTATGT 
AACACTTGCT CCAGAGTCTC CATCCCAATC TCAGCATTAT GTGCCTGTGC CTCTTCTGGA 
TCCCATATGT CCCATTGGGA TAGCAGTCAT AAGGAGAACA ATCAAACTAA AGACATGATT 
ACATACAGTG AAGTTCAGAC AAGTATGCAG GATGAACAAC AAGCTAAAGG CACTATTACA 
TACAGTGATG GTCAGGTAAA AACTCATGTG TTTGCAGAAC AAAAGTCCAA TCACCAAGGC 
ACCAGGAGTA TCAGACAAGA AGGTGGTCAG ATGTCAGCGC TGATCAAGAA TAGCGAAATA 
TCTGAAGACA ATATTACAGA TGATATAAGA TCTCTGTTAA GGCCTCATCC TGAGTGTCCA 
GTTCAGTTTT CGGGCCCAGC CCTTATAAAG GATGTTAGTG AGCTGGAGAG AGTGCAGTGC 
CATTCAACTA AACTGGTAAA AGAGATGGGA GAATTTAGCT ATGAGGAAAG ACAATCAAGT 
GCAAGCTTTC CCCTGCAGCT CTCATTCAAA CAGGGAGTTT CCAAAGACTT GGAGGACATC 
ACTTCAACCT ATGTCTATAG CCCTGGGATT CATACTGCAT GCATAGGCAA TAACAATGTT 
CTGAAGTCTG TGTATACGTA TAAAGGTTTG CAAACTGACA GCCTTCATGC TGATATCATG 
AATGGTAATG AGTGCAAATG CACAGAAGAG ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891119.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746979.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X13, mRNA.

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CloneID	OXa64387
Clone ID Related Accession (Same CDS sequence)	XM_031891119.1
Accession Version	XM_031891119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTG 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCAGGAGGCT CCATCTCAGC CTCTGCATCA 
TCTGCATTGT CCTCTTCTGG ATACCTTGCA TCCTCAATGG AAAGCAGTGT TAAGGATAAA 
GAGCAAACCA AAGACACTTT TACATACATT GAAGCTCAGA TAAACTCTCA GGGCAAAGAG 
CAAACTAAAG ATATTATTAC ATACAGTGAA GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA 
CCTAAAGAGA GTATTACATG TAGTGAAGTT TATAGAAGTA CTCAGGATAA AGAGCAAACT 
AAAGACACAA TTACATACAG TAAAGTTCAG AGAAATATGC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTAGGAAAC 
ACTGGACCAA GTCTGTATGG GGCTTCTTTA GCAGGATTAT CCGAAGCTCC AAGCTTTCCA 
TCATCCATCT CTTCCCCTCC TGTATACCCT GTGTCTCCAA CAGAAAACAG TCAACGGGTA 
ACCTCAGCAG CAATTGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCACAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGCGCAG CCAAGATAAG TACTCAGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACCT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGGAGTA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CTCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746979.1
CDS	441..10070
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891119.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891119.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGAA TTTAGAAAAT 
GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTG 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCAGGAGGCT CCATCTCAGC CTCTGCATCA 
TCTGCATTGT CCTCTTCTGG ATACCTTGCA TCCTCAATGG AAAGCAGTGT TAAGGATAAA 
GAGCAAACCA AAGACACTTT TACATACATT GAAGCTCAGA TAAACTCTCA GGGCAAAGAG 
CAAACTAAAG ATATTATTAC ATACAGTGAA GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA 
CCTAAAGAGA GTATTACATG TAGTGAAGTT TATAGAAGTA CTCAGGATAA AGAGCAAACT 
AAAGACACAA TTACATACAG TAAAGTTCAG AGAAATATGC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTAGGAAAC 
ACTGGACCAA GTCTGTATGG GGCTTCTTTA GCAGGATTAT CCGAAGCTCC AAGCTTTCCA 
TCATCCATCT CTTCCCCTCC TGTATACCCT GTGTCTCCAA CAGAAAACAG TCAACGGGTA 
ACCTCAGCAG CAATTGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCACAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGCGCAG CCAAGATAAG TACTCAGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACCT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGGAGTA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CTCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891120.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746980.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X14, mRNA.

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CloneID	OXa64388
Clone ID Related Accession (Same CDS sequence)	XM_031891120.1
Accession Version	XM_031891120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGTGCAAGC 
TTTCCCCTGC AGCTCTCATT CAAACAGGGA GTTTCCAAAG ACTTGGAGGA CATCACTTCA 
ACCTATGTCT ATAGCCCTGG GATTCATACT GCATGCATAG GCAATAACAA TGTTCTGAAG 
TCTGTGTATA CGTATAAAGG TTTGCAAACT GACAGCCTTC ATGCTGATAT CATGAATGGT 
AATGAGTGCA AATGCACAGA AGAGACCAGC AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746980.1
CDS	441..10034
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDISASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891120.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891120.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGTGCAAGC 
TTTCCCCTGC AGCTCTCATT CAAACAGGGA GTTTCCAAAG ACTTGGAGGA CATCACTTCA 
ACCTATGTCT ATAGCCCTGG GATTCATACT GCATGCATAG GCAATAACAA TGTTCTGAAG 
TCTGTGTATA CGTATAAAGG TTTGCAAACT GACAGCCTTC ATGCTGATAT CATGAATGGT 
AATGAGTGCA AATGCACAGA AGAGACCAGC AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891121.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746981.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X15, mRNA.

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CloneID	OXa64389
Clone ID Related Accession (Same CDS sequence)	XM_031891121.1
Accession Version	XM_031891121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGATCTCTG 
TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG TTTTCGGGCC CAGCCCTTAT AAAGGATGTT 
AGTGAGCTGG AGAGAGTGCA GTGCCATTCA ACTAAACTGG TAAAAGAGAT GGGAGAATTT 
AGCTATGAGG AAAGACAATC AAGTGCAAGC TTTCCCCTGC AGCTCTCATT CAAACAGGGA 
GTTTCCAAAG ACTTGGAGGA CATCACTTCA ACCTATGTCT ATAGCCCTGG GATTCATACT 
GCATGCATAG GCAATAACAA TGTTCTGAAG TCTGTGTATA CGTATAAAGG TTTGCAAACT 
GACAGCCTTC ATGCTGATAT CATGAATGGT AATGAGTGCA AATGCACAGA AGAGACCAGC 
AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746981.1
CDS	441..10004
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891121.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891121.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGTC AGTCTGCTTC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCCC AGCCTCTGCA 
TCGTCCTCTT CTGGATACCT TGCATCCCTA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACTAAAGACA CTATTACATA CACTGAAGCG CAGAGAAATT CTCAGGATAA AGAACAAACT 
AAAGAAACTA TTACATACAG GGAAAGCCAG ATAAATACTC AGAATTTAGA AAATGTTGGC 
CGGTCTGCCT CTAACAAGCA AAATGGGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTGTCCGAA 
GCTACAACTC TATACAAAAA TGTCTCAGGA GGCTCCATCT CAGCCTCTGC ATCATCTGCA 
TTGTCCTCTT CTGGATACCT TGCATCCTCA ATGGAAAGCA GTGTTAAGGA TAAAGAGCAA 
ACCAAAGACA CTTTTACATA CATTGAAGCT CAGATAAACT CTCAGGGCAA AGAGCAAACT 
AAAGATATTA TTACATACAG TGAAGCTCAG AGACATTTTC AGGATAAAGA GCAACCTAAA 
GAGAGTATTA CATGTAGTGA AGTTTATAGA AGTACTCAGG ATAAAGAGCA AACTAAAGAC 
ACAATTACAT ACAGTAAAGT TCAGAGAAAT ATGCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCATCTAGG AAACACTGGA 
CCAAGTCTGT ATGGGGCTTC TTTAGCAGGA TTATCCGAAG CTCCAAGCTT TCCATCATCC 
ATCTCTTCCC CTCCTGTATA CCCTGTGTCT CCAACAGAAA ACAGTCAACG GGTAACCTCA 
GCAGCAATTG ATAAAAAGCA AACTAAAGAC TCTATTACCT ACAGTGTTGG TCAGATGAAT 
ACTCAGGATA AAAAGCAAAC TCTATTAACC TACAGTGTTG GTCAGATAAA GTCTCAGGAT 
ATAAAGCAAA CTAAAGACAC TGTTACATAT AGAGAAGGCC ACATAAACGC ACAGGATGAA 
CAGCAAATGA AAGACTCCAT TACATACAGC GCAGCCAAGA TAAGTACTCA GAATCTAGAA 
AATGTTGGTC GGTCTGCCCC TAACAGGCAA AATGTGATGA GCAGTGTATC TGGGGATCGT 
CTTGGATACT TTGGAGCAAG TCTGTATGGG ACCTCTTCAC ATCTGCCCTG GCTTGCTGGA 
TTAATTGAAG CTCCAAGTCT ATGTAACACT TGCTCCAGAG TCTCCATCCC AATCTCAGCA 
TTATGTGCCT GTGCCTCTTC TGGATCCCAT ATGTCCCATT GGGATAGCAG TCATAAGGAG 
AACAATCAAA CTAAAGACAT GATTACATAC AGTGAAGTTC AGACAAGTAT GCAGGATGAA 
CAACAAGCTA AAGGCACTAT TACATACAGT GATGGTCAGG TAAAAACTCA TGTGTTTGCA 
GAACAAAAGT CCAATCACCA AGGCACCAGG AGTATCAGAC AAGAAGGTGG TCAGATGTCA 
GCGCTGATCA AGAATAGCGA AATATCTGAA GACAATATTA CAGATGATAT AAGATCTCTG 
TTAAGGCCTC ATCCTGAGTG TCCAGTTCAG TTTTCGGGCC CAGCCCTTAT AAAGGATGTT 
AGTGAGCTGG AGAGAGTGCA GTGCCATTCA ACTAAACTGG TAAAAGAGAT GGGAGAATTT 
AGCTATGAGG AAAGACAATC AAGTGCAAGC TTTCCCCTGC AGCTCTCATT CAAACAGGGA 
GTTTCCAAAG ACTTGGAGGA CATCACTTCA ACCTATGTCT ATAGCCCTGG GATTCATACT 
GCATGCATAG GCAATAACAA TGTTCTGAAG TCTGTGTATA CGTATAAAGG TTTGCAAACT 
GACAGCCTTC ATGCTGATAT CATGAATGGT AATGAGTGCA AATGCACAGA AGAGACCAGC 
AATCATCTTA ATGACGGCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891122.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746982.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X16, mRNA.

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CloneID	OXa64390
Clone ID Related Accession (Same CDS sequence)	XM_031891122.1
Accession Version	XM_031891122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCAGAGAT ACAAACTGAG ATACGACCAG GGATACAAAA AGAGATACAG 
CCGGAGATAC AAACAGAGAT ACAAACTGAG ATACGACCAG GGATACAAAA AGAGACACGA 
CGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACATACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GACACTATTA CATACAGTGA CGTTCAGATA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTATTACA 
TTCAGTGAAG GTCAGAGACA TACACAGAAT AAAAAACAGT CTGATGATGC TATTACATAC 
AGTAAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GACGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAC 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGG CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGACCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGTAT CCCCAATGGA AAGCAGTCTT AAGAATTTAG AAAGTGTTGG TCAGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGAA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCTGG AGTCTCCATC TCAGCCTCTG CATCATCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCC AATGGAAAGC AGTGTTAAGA ATTTAGAAAA TGTTGGTCAG 
TCTGCTTCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCCCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCTAAT GGAAAGCAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCGCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TGTCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCTGCATT GTCCTCTTCT 
GGATACCTTG CATCCTCAAT GGAAAGCAGT GTTAAGGATA AAGAGCAAAC CAAAGACACT 
TTTACATACA TTGAAGCTCA GATAAACTCT CAGGGCAAAG AGCAAACTAA AGATATTATT 
ACATACAGTG AAGCTCAGAG ACATTTTCAG GATAAAGAGC AACCTAAAGA GAGTATTACA 
TGTAGTGAAG TTTATAGAAG TACTCAGGAT AAAGAGCAAA CTAAAGACAC AATTACATAC 
AGTAAAGTTC AGAGAAATAT GCAGAATCTA GAAAATGTTG GTCGGTCTGC CCCTAACAGG 
CAAAATGTGA TGAGCAGTGT ATCTGGGGAT CATCTAGGAA ACACTGGACC AAGTCTGTAT 
GGGGCTTCTT TAGCAGGATT ATCCGAAGCT CCAAGCTTTC CATCATCCAT CTCTTCCCCT 
CCTGTATACC CTGTGTCTCC AACAGAAAAC AGTCAACGGG TAACCTCAGC AGCAATTGAT 
AAAAAGCAAA CTAAAGACTC TATTACCTAC AGTGTTGGTC AGATGAATAC TCAGGATAAA 
AAGCAAACTC TATTAACCTA CAGTGTTGGT CAGATAAAGT CTCAGGATAT AAAGCAAACT 
AAAGACACTG TTACATATAG AGAAGGCCAC ATAAACGCAC AGGATGAACA GCAAATGAAA 
GACTCCATTA CATACAGCGC AGCCAAGATA AGTACTCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCGTCT TGGATACTTT 
GGAGCAAGTC TGTATGGGAC CTCTTCACAT CTGCCCTGGC TTGCTGGATT AATTGAAGCT 
CCAAGTCTAT GTAACACTTG CTCCAGAGTC TCCATCCCAA TCTCAGCATT ATGTGCCTGT 
GCCTCTTCTG GATCCCATAT GTCCCATTGG GATAGCAGTC ATAAGGAGAA CAATCAAACT 
AAAGACATGA TTACATACAG TGAAGTTCAG ACAAGTATGC AGGATGAACA ACAAGCTAAA 
GGCACTATTA CATACAGTGA TGGTCAGGTA AAAACTCATG TGTTTGCAGA ACAAAAGTCC 
AATCACCAAG GCACCAGGAG TATCAGACAA GAAGGTGGTC AGATGTCAGC GCTGATCAAG 
AATAGCGAAA TATCTGAAGA CAATATTACA GATGATATAA GATCTCTGTT AAGGCCTCAT 
CCTGAGTGTC CAGTTCAGTT TTCGGGCCCA GCCCTTATAA AGGATGTTAG TGAGCTGGAG 
AGAGTGCAGT GCCATTCAAC TAAACTGGTA AAAGAGATGG GAGAATTTAG CTATGAGGAA 
AGACAATCAA GTGCAAGCTT TCCCCTGCAG CTCTCATTCA AACAGGGAGT TTCCAAAGAC 
TTGGAGGACA TCACTTCAAC CTATGTCTAT AGCCCTGGGA TTCATACTGC ATGCATAGGC 
AATAACAATG TTCTGAAGTC TGTGTATACG TATAAAGGTT TGCAAACTGA CAGCCTTCAT 
GCTGATATCA TGAATGGTAA TGAGTGCAAA TGCACAGAAG AGACCAGCAA TCATCTTAAT 
GACGGCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746982.1
CDS	441..9992
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891122.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891122.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCAGAGAT ACAAACTGAG ATACGACCAG GGATACAAAA AGAGATACAG 
CCGGAGATAC AAACAGAGAT ACAAACTGAG ATACGACCAG GGATACAAAA AGAGACACGA 
CGAAAGATAC ATAAAGAGAT AGAACCAAAT ATACAAACAG AGATAGAAAA AGAAATGCAA 
CCAGACATAC GACCAGAAAT ACATACAGAG ATAGAAAAAG ATATTCAACC AGAGATACAA 
TCAGAAACAC CGAGAGAGGC ACAAATAAAA GGACCGAAAA AAGATTCTCG TATACTGACA 
GTAATAATTG CAGTTTCGAT ACTGTGCGGA ATATTTGTTG GTATAGCTCT TTATTATGGA 
ATAAGACGAC GACTAAAGGT TAAAAATCAG TCTAATTGCA CTATTACATA CAGTGAAGTT 
CTGACAAGTA CTCAGGATAA AGAGCATATT AAAGACTCTA TTACATACAG TGAAGTTCAG 
ATAAATACTC AGGATAAAGT GCATACTAAA GACACTATTA CATACAGTGA CGTTCAGATA 
AATTCTAGGA GAAATATGCA GGGCAAAGAA GAACCTGATT ATAATGATTA TACTATTACA 
TTCAGTGAAG GTCAGAGACA TACACAGAAT AAAAAACAGT CTGATGATGC TATTACATAC 
AGTAAAGTTC AGATAAATAC TCAGGATAAA GAGCATATTA AAGACTCTAT TACTTACAGT 
GACGTTCAGA TAAATACTCA GGATAAAGTG CATACTAAAG ACACTATTAC ATACAGTGAC 
GTTCTGATGA ATTCTAGGAG AAATATGCAG GATAAAGAGC AAACTAAAGG CACTATTACA 
TACACTGAAG CGCAGAGACA TTCTCAGAAT TTAGAAAATG TTGACCAGTC TGCATCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCTCAGCC TCTGCATCAT CTGCATCATC CTCTTCTGGA 
TACCTTGTAT CCCCAATGGA AAGCAGTCTT AAGAATTTAG AAAGTGTTGG TCAGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGAA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCTGG AGTCTCCATC TCAGCCTCTG CATCATCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCC AATGGAAAGC AGTGTTAAGA ATTTAGAAAA TGTTGGTCAG 
TCTGCTTCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCCCAG CCTCTGCATC GTCCTCTTCT 
GGATACCTTG CATCCCTAAT GGAAAGCAGT GTTAAGGATA AAGAGCAAAC TAAAGACACT 
ATTACATACA CTGAAGCGCA GAGAAATTCT CAGGATAAAG AACAAACTAA AGAAACTATT 
ACATACAGGG AAAGCCAGAT AAATACTCAG AATTTAGAAA ATGTTGGCCG GTCTGCCTCT 
AACAAGCAAA ATGGGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TGTCCGAAGC TACAACTCTA 
TACAAAAATG TCTCAGGAGG CTCCATCTCA GCCTCTGCAT CATCTGCATT GTCCTCTTCT 
GGATACCTTG CATCCTCAAT GGAAAGCAGT GTTAAGGATA AAGAGCAAAC CAAAGACACT 
TTTACATACA TTGAAGCTCA GATAAACTCT CAGGGCAAAG AGCAAACTAA AGATATTATT 
ACATACAGTG AAGCTCAGAG ACATTTTCAG GATAAAGAGC AACCTAAAGA GAGTATTACA 
TGTAGTGAAG TTTATAGAAG TACTCAGGAT AAAGAGCAAA CTAAAGACAC AATTACATAC 
AGTAAAGTTC AGAGAAATAT GCAGAATCTA GAAAATGTTG GTCGGTCTGC CCCTAACAGG 
CAAAATGTGA TGAGCAGTGT ATCTGGGGAT CATCTAGGAA ACACTGGACC AAGTCTGTAT 
GGGGCTTCTT TAGCAGGATT ATCCGAAGCT CCAAGCTTTC CATCATCCAT CTCTTCCCCT 
CCTGTATACC CTGTGTCTCC AACAGAAAAC AGTCAACGGG TAACCTCAGC AGCAATTGAT 
AAAAAGCAAA CTAAAGACTC TATTACCTAC AGTGTTGGTC AGATGAATAC TCAGGATAAA 
AAGCAAACTC TATTAACCTA CAGTGTTGGT CAGATAAAGT CTCAGGATAT AAAGCAAACT 
AAAGACACTG TTACATATAG AGAAGGCCAC ATAAACGCAC AGGATGAACA GCAAATGAAA 
GACTCCATTA CATACAGCGC AGCCAAGATA AGTACTCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCGTCT TGGATACTTT 
GGAGCAAGTC TGTATGGGAC CTCTTCACAT CTGCCCTGGC TTGCTGGATT AATTGAAGCT 
CCAAGTCTAT GTAACACTTG CTCCAGAGTC TCCATCCCAA TCTCAGCATT ATGTGCCTGT 
GCCTCTTCTG GATCCCATAT GTCCCATTGG GATAGCAGTC ATAAGGAGAA CAATCAAACT 
AAAGACATGA TTACATACAG TGAAGTTCAG ACAAGTATGC AGGATGAACA ACAAGCTAAA 
GGCACTATTA CATACAGTGA TGGTCAGGTA AAAACTCATG TGTTTGCAGA ACAAAAGTCC 
AATCACCAAG GCACCAGGAG TATCAGACAA GAAGGTGGTC AGATGTCAGC GCTGATCAAG 
AATAGCGAAA TATCTGAAGA CAATATTACA GATGATATAA GATCTCTGTT AAGGCCTCAT 
CCTGAGTGTC CAGTTCAGTT TTCGGGCCCA GCCCTTATAA AGGATGTTAG TGAGCTGGAG 
AGAGTGCAGT GCCATTCAAC TAAACTGGTA AAAGAGATGG GAGAATTTAG CTATGAGGAA 
AGACAATCAA GTGCAAGCTT TCCCCTGCAG CTCTCATTCA AACAGGGAGT TTCCAAAGAC 
TTGGAGGACA TCACTTCAAC CTATGTCTAT AGCCCTGGGA TTCATACTGC ATGCATAGGC 
AATAACAATG TTCTGAAGTC TGTGTATACG TATAAAGGTT TGCAAACTGA CAGCCTTCAT 
GCTGATATCA TGAATGGTAA TGAGTGCAAA TGCACAGAAG AGACCAGCAA TCATCTTAAT 
GACGGCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891123.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746983.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X17, mRNA.

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CloneID	OXa64391
Clone ID Related Accession (Same CDS sequence)	XM_031891123.1
Accession Version	XM_031891123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAGAG AGAGATACAG 
CCAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGATACAGCC GGAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGACACGACG AAAGATACAT 
AAAGAGATAG AACCAAATAT ACAAACAGAG ATAGAAAAAG AAATGCAACC AGACATACGA 
CCAGAAATAC ATACAGAGAT AGAAAAAGAT ATTCAACCAG AGATACAATC AGAAACACCG 
AGAGAGGCAC AAATAAAAGG ACCGAAAAAA GATTCTCGTA TACTGACAGT AATAATTGCA 
GTTTCGATAC TGTGCGGAAT ATTTGTTGGT ATAGCTCTTT ATTATGGAAT AAGACGACGA 
CTAAAGGTTA AAAATCAGTC TAATTGCACT ATTACATACA GTGAAGTTCT GACAAGTACT 
CAGGATAAAG AGCATATTAA AGACTCTATT ACATACAGTG AAGTTCAGAT AAATACTCAG 
GATAAAGTGC ATACTAAAGA CACTATTACA TACAGTGACG TTCAGATAAA TTCTAGGAGA 
AATATGCAGG GCAAAGAAGA ACCTGATTAT AATGATTATA CTATTACATT CAGTGAAGGT 
CAGAGACATA CACAGAATAA AAAACAGTCT GATGATGCTA TTACATACAG TAAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA CGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGACGT TCTGATGAAT 
TCTAGGAGAA ATATGCAGGA TAAAGAGCAA ACTAAAGGCA CTATTACATA CACTGAAGCG 
CAGAGACATT CTCAGAATTT AGAAAATGTT GACCAGTCTG CATCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCTCAGCCTC TGCATCATCT GCATCATCCT CTTCTGGATA CCTTGTATCC 
CCAATGGAAA GCAGTCTTAA GAATTTAGAA AGTGTTGGTC AGTCTGCCTC TAACAAGCAA 
AATGGGATGA GCAGTGAATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCTGGAG TCTCCATCTC AGCCTCTGCA TCATCTGCAT CGTCCTCTTC TGGATACCTT 
GCATCCCCAA TGGAAAGCAG TGTTAAGAAT TTAGAAAATG TTGGTCAGTC TGCTTCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCCCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCTAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCGCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG GGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TCTTTACATC AGACCAGATC TGCAGGATTG TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCTGCATTGT CCTCTTCTGG ATACCTTGCA 
TCCTCAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACCA AAGACACTTT TACATACATT 
GAAGCTCAGA TAAACTCTCA GGGCAAAGAG CAAACTAAAG ATATTATTAC ATACAGTGAA 
GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA CCTAAAGAGA GTATTACATG TAGTGAAGTT 
TATAGAAGTA CTCAGGATAA AGAGCAAACT AAAGACACAA TTACATACAG TAAAGTTCAG 
AGAAATATGC AGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCA TCTAGGAAAC ACTGGACCAA GTCTGTATGG GGCTTCTTTA 
GCAGGATTAT CCGAAGCTCC AAGCTTTCCA TCATCCATCT CTTCCCCTCC TGTATACCCT 
GTGTCTCCAA CAGAAAACAG TCAACGGGTA ACCTCAGCAG CAATTGATAA AAAGCAAACT 
AAAGACTCTA TTACCTACAG TGTTGGTCAG ATGAATACTC AGGATAAAAA GCAAACTCTA 
TTAACCTACA GTGTTGGTCA GATAAAGTCT CAGGATATAA AGCAAACTAA AGACACTGTT 
ACATATAGAG AAGGCCACAT AAACGCACAG GATGAACAGC AAATGAAAGA CTCCATTACA 
TACAGCGCAG CCAAGATAAG TACTCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCGTCTTG GATACTTTGG AGCAAGTCTG 
TATGGGACCT CTTCACATCT GCCCTGGCTT GCTGGATTAA TTGAAGCTCC AAGTCTATGT 
AACACTTGCT CCAGAGTCTC CATCCCAATC TCAGCATTAT GTGCCTGTGC CTCTTCTGGA 
TCCCATATGT CCCATTGGGA TAGCAGTCAT AAGGAGAACA ATCAAACTAA AGACATGATT 
ACATACAGTG AAGTTCAGAC AAGTATGCAG GATGAACAAC AAGCTAAAGG CACTATTACA 
TACAGTGATG GTCAGGTAAA AACTCATGTG TTTGCAGAAC AAAAGTCCAA TCACCAAGGC 
ACCAGGAGTA TCAGACAAGA AGGTGGTCAG ATGTCAGCGC TGATCAAGAA TAGCGAAATA 
TCTGAAGACA ATATTACAGA TGATATAAGA TCTCTGTTAA GGCCTCATCC TGAGTGTCCA 
GTTCAGTTTT CGGGCCCAGC CCTTATAAAG GATGTTAGTG AGCTGGAGAG AGTGCAGTGC 
CATTCAACTA AACTGGTAAA AGAGATGGGA GAATTTAGCT ATGAGGAAAG ACAATCAAGT 
GCAAGCTTTC CCCTGCAGCT CTCATTCAAA CAGGGAGTTT CCAAAGACTT GGAGGACATC 
ACTTCAACCT ATGTCTATAG CCCTGGGATT CATACTGCAT GCATAGGCAA TAACAATGTT 
CTGAAGTCTG TGTATACGTA TAAAGGTTTG CAAACTGACA GCCTTCATGC TGATATCATG 
AATGGTAATG AGTGCAAATG CACAGAAGAG ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746983.1
CDS	441..9980
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891123.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891123.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAACTG AGATACGACC AGGGATACAG 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAGAG AGAGATACAG 
CCAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGATACAGCC GGAGATACAA 
ACAGAGATAC AAACTGAGAT ACGACCAGGG ATACAAAAAG AGACACGACG AAAGATACAT 
AAAGAGATAG AACCAAATAT ACAAACAGAG ATAGAAAAAG AAATGCAACC AGACATACGA 
CCAGAAATAC ATACAGAGAT AGAAAAAGAT ATTCAACCAG AGATACAATC AGAAACACCG 
AGAGAGGCAC AAATAAAAGG ACCGAAAAAA GATTCTCGTA TACTGACAGT AATAATTGCA 
GTTTCGATAC TGTGCGGAAT ATTTGTTGGT ATAGCTCTTT ATTATGGAAT AAGACGACGA 
CTAAAGGTTA AAAATCAGTC TAATTGCACT ATTACATACA GTGAAGTTCT GACAAGTACT 
CAGGATAAAG AGCATATTAA AGACTCTATT ACATACAGTG AAGTTCAGAT AAATACTCAG 
GATAAAGTGC ATACTAAAGA CACTATTACA TACAGTGACG TTCAGATAAA TTCTAGGAGA 
AATATGCAGG GCAAAGAAGA ACCTGATTAT AATGATTATA CTATTACATT CAGTGAAGGT 
CAGAGACATA CACAGAATAA AAAACAGTCT GATGATGCTA TTACATACAG TAAAGTTCAG 
ATAAATACTC AGGATAAAGA GCATATTAAA GACTCTATTA CTTACAGTGA CGTTCAGATA 
AATACTCAGG ATAAAGTGCA TACTAAAGAC ACTATTACAT ACAGTGACGT TCTGATGAAT 
TCTAGGAGAA ATATGCAGGA TAAAGAGCAA ACTAAAGGCA CTATTACATA CACTGAAGCG 
CAGAGACATT CTCAGAATTT AGAAAATGTT GACCAGTCTG CATCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCTCAGCCTC TGCATCATCT GCATCATCCT CTTCTGGATA CCTTGTATCC 
CCAATGGAAA GCAGTCTTAA GAATTTAGAA AGTGTTGGTC AGTCTGCCTC TAACAAGCAA 
AATGGGATGA GCAGTGAATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCTGGAG TCTCCATCTC AGCCTCTGCA TCATCTGCAT CGTCCTCTTC TGGATACCTT 
GCATCCCCAA TGGAAAGCAG TGTTAAGAAT TTAGAAAATG TTGGTCAGTC TGCTTCTAAC 
AAGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCCCAGCC TCTGCATCGT CCTCTTCTGG ATACCTTGCA 
TCCCTAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACTA AAGACACTAT TACATACACT 
GAAGCGCAGA GAAATTCTCA GGATAAAGAA CAAACTAAAG AAACTATTAC ATACAGGGAA 
AGCCAGATAA ATACTCAGAA TTTAGAAAAT GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT 
GGGATGAGCA GTGTATCTGG GGATCATCTT GGATACTCTG GGTCAGGTCT ATATGGCGCC 
TCTTTACATC AGACCAGATC TGCAGGATTG TCCGAAGCTA CAACTCTATA CAAAAATGTC 
TCAGGAGGCT CCATCTCAGC CTCTGCATCA TCTGCATTGT CCTCTTCTGG ATACCTTGCA 
TCCTCAATGG AAAGCAGTGT TAAGGATAAA GAGCAAACCA AAGACACTTT TACATACATT 
GAAGCTCAGA TAAACTCTCA GGGCAAAGAG CAAACTAAAG ATATTATTAC ATACAGTGAA 
GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA CCTAAAGAGA GTATTACATG TAGTGAAGTT 
TATAGAAGTA CTCAGGATAA AGAGCAAACT AAAGACACAA TTACATACAG TAAAGTTCAG 
AGAAATATGC AGAATCTAGA AAATGTTGGT CGGTCTGCCC CTAACAGGCA AAATGTGATG 
AGCAGTGTAT CTGGGGATCA TCTAGGAAAC ACTGGACCAA GTCTGTATGG GGCTTCTTTA 
GCAGGATTAT CCGAAGCTCC AAGCTTTCCA TCATCCATCT CTTCCCCTCC TGTATACCCT 
GTGTCTCCAA CAGAAAACAG TCAACGGGTA ACCTCAGCAG CAATTGATAA AAAGCAAACT 
AAAGACTCTA TTACCTACAG TGTTGGTCAG ATGAATACTC AGGATAAAAA GCAAACTCTA 
TTAACCTACA GTGTTGGTCA GATAAAGTCT CAGGATATAA AGCAAACTAA AGACACTGTT 
ACATATAGAG AAGGCCACAT AAACGCACAG GATGAACAGC AAATGAAAGA CTCCATTACA 
TACAGCGCAG CCAAGATAAG TACTCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCGTCTTG GATACTTTGG AGCAAGTCTG 
TATGGGACCT CTTCACATCT GCCCTGGCTT GCTGGATTAA TTGAAGCTCC AAGTCTATGT 
AACACTTGCT CCAGAGTCTC CATCCCAATC TCAGCATTAT GTGCCTGTGC CTCTTCTGGA 
TCCCATATGT CCCATTGGGA TAGCAGTCAT AAGGAGAACA ATCAAACTAA AGACATGATT 
ACATACAGTG AAGTTCAGAC AAGTATGCAG GATGAACAAC AAGCTAAAGG CACTATTACA 
TACAGTGATG GTCAGGTAAA AACTCATGTG TTTGCAGAAC AAAAGTCCAA TCACCAAGGC 
ACCAGGAGTA TCAGACAAGA AGGTGGTCAG ATGTCAGCGC TGATCAAGAA TAGCGAAATA 
TCTGAAGACA ATATTACAGA TGATATAAGA TCTCTGTTAA GGCCTCATCC TGAGTGTCCA 
GTTCAGTTTT CGGGCCCAGC CCTTATAAAG GATGTTAGTG AGCTGGAGAG AGTGCAGTGC 
CATTCAACTA AACTGGTAAA AGAGATGGGA GAATTTAGCT ATGAGGAAAG ACAATCAAGT 
GCAAGCTTTC CCCTGCAGCT CTCATTCAAA CAGGGAGTTT CCAAAGACTT GGAGGACATC 
ACTTCAACCT ATGTCTATAG CCCTGGGATT CATACTGCAT GCATAGGCAA TAACAATGTT 
CTGAAGTCTG TGTATACGTA TAAAGGTTTG CAAACTGACA GCCTTCATGC TGATATCATG 
AATGGTAATG AGTGCAAATG CACAGAAGAG ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891124.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746984.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X18, mRNA.

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CloneID	OXa64392
Clone ID Related Accession (Same CDS sequence)	XM_031891124.1
Accession Version	XM_031891124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACTAAAG ACACTATTAC ATACACTGAA GCGCAGAGAA ATTCTCAGGA TAAAGAACAA 
ACTAAAGAAA CTATTACATA CAGGGAAAGC CAGATAAATA CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746984.1
CDS	441..9947
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891124.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891124.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACTAAAG ACACTATTAC ATACACTGAA GCGCAGAGAA ATTCTCAGGA TAAAGAACAA 
ACTAAAGAAA CTATTACATA CAGGGAAAGC CAGATAAATA CTCAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891125.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746985.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X19, mRNA.

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CloneID	OXa64393
Clone ID Related Accession (Same CDS sequence)	XM_031891125.1
Accession Version	XM_031891125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGGCACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGACCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCTGGAGT CTCCATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG TATCCCCAAT GGAAAGCAGT CTTAAGAATT TAGAAAGTGT TGGTCAGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GAATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AGCAGTGTTA AGAATTTAGA AAATGTTGGT 
CAGTCTGCTT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCC CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCT AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC GCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTGTCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCTGC ATTGTCCTCT 
TCTGGATACC TTGCATCCTC AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACCAAAGAC 
ACTTTTACAT ACATTGAAGC TCAGATAAAC TCTCAGGGCA AAGAGCAAAC TAAAGATATT 
ATTACATACA GTGAAGCTCA GAGACATTTT CAGGATAAAG AGCAACCTAA AGAGAGTATT 
ACATGTAGTG AAGTTTATAG AAGTACTCAG GATAAAGAGC AAACTAAAGA CACAATTACA 
TACAGTAAAG TTCAGAGAAA TATGCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTAG GAAACACTGG ACCAAGTCTG 
TATGGGGCTT CTTTAGCAGG ATTATCCGAA GCTCCAAGCT TTCCATCATC CATCTCTTCC 
CCTCCTGTAT ACCCTGTGTC TCCAACAGAA AACAGTCAAC GGGTAACCTC AGCAGCAATT 
GATAAAAAGC AAACTAAAGA CTCTATTACC TACAGTGTTG GTCAGATGAA TACTCAGGAT 
AAAAAGCAAA CTCTATTAAC CTACAGTGTT GGTCAGATAA AGTCTCAGGA TATAAAGCAA 
ACTAAAGACA CTGTTACATA TAGAGAAGGC CACATAAACG CACAGGATGA ACAGCAAATG 
AAAGACTCCA TTACATACAG CGCAGCCAAG ATAAGTACTC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCG TCTTGGATAC 
TTTGGAGCAA GTCTGTATGG GACCTCTTCA CATCTGCCCT GGCTTGCTGG ATTAATTGAA 
GCTCCAAGTC TATGTAACAC TTGCTCCAGA GTCTCCATCC CAATCTCAGC ATTATGTGCC 
TGTGCCTCTT CTGGATCCCA TATGTCCCAT TGGGATAGCA GTCATAAGGA GAACAATCAA 
ACTAAAGACA TGATTACATA CAGTGAAGTT CAGACAAGTA TGCAGGATGA ACAACAAGCT 
AAAGGCACTA TTACATACAG TGATGGTCAG GTAAAAACTC ATGTGTTTGC AGAACAAAAG 
TCCAATCACC AAGGCACCAG GAGTATCAGA CAAGAAGGTG GTCAGATGTC AGCGCTGATC 
AAGAATAGCG AAATATCTGA AGACAATATT ACAGATGATA TAAGATCTCT GTTAAGGCCT 
CATCCTGAGT GTCCAGTTCA GTTTTCGGGC CCAGCCCTTA TAAAGGATGT TAGTGAGCTG 
GAGAGAGTGC AGTGCCATTC AACTAAACTG GTAAAAGAGA TGGGAGAATT TAGCTATGAG 
GAAAGACAAT CAAGTGCAAG CTTTCCCCTG CAGCTCTCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACCAG CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746985.1
CDS	441..9935
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891125.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891125.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGGCACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGACCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCTGGAGT CTCCATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG TATCCCCAAT GGAAAGCAGT CTTAAGAATT TAGAAAGTGT TGGTCAGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GAATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AGCAGTGTTA AGAATTTAGA AAATGTTGGT 
CAGTCTGCTT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCC CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCT AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC GCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTGTCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCTGC ATTGTCCTCT 
TCTGGATACC TTGCATCCTC AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACCAAAGAC 
ACTTTTACAT ACATTGAAGC TCAGATAAAC TCTCAGGGCA AAGAGCAAAC TAAAGATATT 
ATTACATACA GTGAAGCTCA GAGACATTTT CAGGATAAAG AGCAACCTAA AGAGAGTATT 
ACATGTAGTG AAGTTTATAG AAGTACTCAG GATAAAGAGC AAACTAAAGA CACAATTACA 
TACAGTAAAG TTCAGAGAAA TATGCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTAG GAAACACTGG ACCAAGTCTG 
TATGGGGCTT CTTTAGCAGG ATTATCCGAA GCTCCAAGCT TTCCATCATC CATCTCTTCC 
CCTCCTGTAT ACCCTGTGTC TCCAACAGAA AACAGTCAAC GGGTAACCTC AGCAGCAATT 
GATAAAAAGC AAACTAAAGA CTCTATTACC TACAGTGTTG GTCAGATGAA TACTCAGGAT 
AAAAAGCAAA CTCTATTAAC CTACAGTGTT GGTCAGATAA AGTCTCAGGA TATAAAGCAA 
ACTAAAGACA CTGTTACATA TAGAGAAGGC CACATAAACG CACAGGATGA ACAGCAAATG 
AAAGACTCCA TTACATACAG CGCAGCCAAG ATAAGTACTC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCG TCTTGGATAC 
TTTGGAGCAA GTCTGTATGG GACCTCTTCA CATCTGCCCT GGCTTGCTGG ATTAATTGAA 
GCTCCAAGTC TATGTAACAC TTGCTCCAGA GTCTCCATCC CAATCTCAGC ATTATGTGCC 
TGTGCCTCTT CTGGATCCCA TATGTCCCAT TGGGATAGCA GTCATAAGGA GAACAATCAA 
ACTAAAGACA TGATTACATA CAGTGAAGTT CAGACAAGTA TGCAGGATGA ACAACAAGCT 
AAAGGCACTA TTACATACAG TGATGGTCAG GTAAAAACTC ATGTGTTTGC AGAACAAAAG 
TCCAATCACC AAGGCACCAG GAGTATCAGA CAAGAAGGTG GTCAGATGTC AGCGCTGATC 
AAGAATAGCG AAATATCTGA AGACAATATT ACAGATGATA TAAGATCTCT GTTAAGGCCT 
CATCCTGAGT GTCCAGTTCA GTTTTCGGGC CCAGCCCTTA TAAAGGATGT TAGTGAGCTG 
GAGAGAGTGC AGTGCCATTC AACTAAACTG GTAAAAGAGA TGGGAGAATT TAGCTATGAG 
GAAAGACAAT CAAGTGCAAG CTTTCCCCTG CAGCTCTCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACCAG CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891127.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746987.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X20, mRNA.

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CloneID	OXa64394
Clone ID Related Accession (Same CDS sequence)	XM_031891127.1
Accession Version	XM_031891127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGACGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCTGA TGAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGGCACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGACCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCTGGAGT CTCCATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG TATCCCCAAT GGAAAGCAGT CTTAAGAATT TAGAAAGTGT TGGTCAGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GAATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AGCAGTGTTA AGAATTTAGA AAATGTTGGT 
CAGTCTGCTT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCC CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCT AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC GCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTGTCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCTGC ATTGTCCTCT 
TCTGGATACC TTGCATCCTC AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACCAAAGAC 
ACTTTTACAT ACATTGAAGC TCAGATAAAC TCTCAGGGCA AAGAGCAAAC TAAAGATATT 
ATTACATACA GTGAAGCTCA GAGACATTTT CAGGATAAAG AGCAACCTAA AGAGAGTATT 
ACATGTAGTG AAGTTTATAG AAGTACTCAG GATAAAGAGC AAACTAAAGA CACAATTACA 
TACAGTAAAG TTCAGAGAAA TATGCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTAG GAAACACTGG ACCAAGTCTG 
TATGGGGCTT CTTTAGCAGG ATTATCCGAA GCTCCAAGCT TTCCATCATC CATCTCTTCC 
CCTCCTGTAT ACCCTGTGTC TCCAACAGAA AACAGTCAAC GGGTAACCTC AGCAGCAATT 
GATAAAAAGC AAACTAAAGA CTCTATTACC TACAGTGTTG GTCAGATGAA TACTCAGGAT 
AAAAAGCAAA CTCTATTAAC CTACAGTGTT GGTCAGATAA AGTCTCAGGA TATAAAGCAA 
ACTAAAGACA CTGTTACATA TAGAGAAGGC CACATAAACG CACAGGATGA ACAGCAAATG 
AAAGACTCCA TTACATACAG CGCAGCCAAG ATAAGTACTC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCG TCTTGGATAC 
TTTGGAGCAA GTCTGTATGG GACCTCTTCA CATCTGCCCT GGCTTGCTGG ATTAATTGAA 
GCTCCAAGTC TATGTAACAC TTGCTCCAGA GTCTCCATCC CAATCTCAGC ATTATGTGCC 
TGTGCCTCTT CTGGATCCCA TATGTCCCAT TGGGATAGCA GTCATAAGGA GAACAATCAA 
ACTAAAGACA TGATTACATA CAGTGAAGTT CAGACAAGTA TGCAGGATGA ACAACAAGCT 
AAAGGCACTA TTACATACAG TGATGGTCAG GTAAAAACTC ATGTGTTTGC AGAACAAAAG 
TCCAATCACC AAGGCACCAG GAGTATCAGA CAAGAAGGTG GTCAGATGTC AGCGCTGATC 
AAGAATAGCG AAATATCTGA AGACAATATT ACAGATGATA TAAGATCTCT GTTAAGGCCT 
CATCCTGAGT GTCCAGTTCA GTTTTCGGGC CCAGCCCTTA TAAAGGATGT TAGTGAGCTG 
GAGAGAGTGC AGTGCCATTC AACTAAACTG GTAAAAGAGA TGGGAGAATT TAGCTATGAG 
GAAAGACAAT CAAGTGCAAG CTTTCCCCTG CAGCTCTCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACCAG CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746987.1
CDS	441..9935
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891127.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891127.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACTTAC 
AGTGACGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCTGA TGAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGGCACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGACCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCTGGAGT CTCCATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG TATCCCCAAT GGAAAGCAGT CTTAAGAATT TAGAAAGTGT TGGTCAGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GAATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AGCAGTGTTA AGAATTTAGA AAATGTTGGT 
CAGTCTGCTT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCC CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCT AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC GCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTGTCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCTGC ATTGTCCTCT 
TCTGGATACC TTGCATCCTC AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACCAAAGAC 
ACTTTTACAT ACATTGAAGC TCAGATAAAC TCTCAGGGCA AAGAGCAAAC TAAAGATATT 
ATTACATACA GTGAAGCTCA GAGACATTTT CAGGATAAAG AGCAACCTAA AGAGAGTATT 
ACATGTAGTG AAGTTTATAG AAGTACTCAG GATAAAGAGC AAACTAAAGA CACAATTACA 
TACAGTAAAG TTCAGAGAAA TATGCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTAG GAAACACTGG ACCAAGTCTG 
TATGGGGCTT CTTTAGCAGG ATTATCCGAA GCTCCAAGCT TTCCATCATC CATCTCTTCC 
CCTCCTGTAT ACCCTGTGTC TCCAACAGAA AACAGTCAAC GGGTAACCTC AGCAGCAATT 
GATAAAAAGC AAACTAAAGA CTCTATTACC TACAGTGTTG GTCAGATGAA TACTCAGGAT 
AAAAAGCAAA CTCTATTAAC CTACAGTGTT GGTCAGATAA AGTCTCAGGA TATAAAGCAA 
ACTAAAGACA CTGTTACATA TAGAGAAGGC CACATAAACG CACAGGATGA ACAGCAAATG 
AAAGACTCCA TTACATACAG CGCAGCCAAG ATAAGTACTC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCG TCTTGGATAC 
TTTGGAGCAA GTCTGTATGG GACCTCTTCA CATCTGCCCT GGCTTGCTGG ATTAATTGAA 
GCTCCAAGTC TATGTAACAC TTGCTCCAGA GTCTCCATCC CAATCTCAGC ATTATGTGCC 
TGTGCCTCTT CTGGATCCCA TATGTCCCAT TGGGATAGCA GTCATAAGGA GAACAATCAA 
ACTAAAGACA TGATTACATA CAGTGAAGTT CAGACAAGTA TGCAGGATGA ACAACAAGCT 
AAAGGCACTA TTACATACAG TGATGGTCAG GTAAAAACTC ATGTGTTTGC AGAACAAAAG 
TCCAATCACC AAGGCACCAG GAGTATCAGA CAAGAAGGTG GTCAGATGTC AGCGCTGATC 
AAGAATAGCG AAATATCTGA AGACAATATT ACAGATGATA TAAGATCTCT GTTAAGGCCT 
CATCCTGAGT GTCCAGTTCA GTTTTCGGGC CCAGCCCTTA TAAAGGATGT TAGTGAGCTG 
GAGAGAGTGC AGTGCCATTC AACTAAACTG GTAAAAGAGA TGGGAGAATT TAGCTATGAG 
GAAAGACAAT CAAGTGCAAG CTTTCCCCTG CAGCTCTCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACCAG CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891128.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746988.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X21, mRNA.

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CloneID	OXa64395
Clone ID Related Accession (Same CDS sequence)	XM_031891128.1
Accession Version	XM_031891128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCTGA TGAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGGCACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGACCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCTGGAGT CTCCATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG TATCCCCAAT GGAAAGCAGT CTTAAGAATT TAGAAAGTGT TGGTCAGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GAATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AGCAGTGTTA AGAATTTAGA AAATGTTGGT 
CAGTCTGCTT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCC CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCT AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC GCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTGTCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCTGC ATTGTCCTCT 
TCTGGATACC TTGCATCCTC AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACCAAAGAC 
ACTTTTACAT ACATTGAAGC TCAGATAAAC TCTCAGGGCA AAGAGCAAAC TAAAGATATT 
ATTACATACA GTGAAGCTCA GAGACATTTT CAGGATAAAG AGCAACCTAA AGAGAGTATT 
ACATGTAGTG AAGTTTATAG AAGTACTCAG GATAAAGAGC AAACTAAAGA CACAATTACA 
TACAGTAAAG TTCAGAGAAA TATGCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTAG GAAACACTGG ACCAAGTCTG 
TATGGGGCTT CTTTAGCAGG ATTATCCGAA GCTCCAAGCT TTCCATCATC CATCTCTTCC 
CCTCCTGTAT ACCCTGTGTC TCCAACAGAA AACAGTCAAC GGGTAACCTC AGCAGCAATT 
GATAAAAAGC AAACTAAAGA CTCTATTACC TACAGTGTTG GTCAGATGAA TACTCAGGAT 
AAAAAGCAAA CTCTATTAAC CTACAGTGTT GGTCAGATAA AGTCTCAGGA TATAAAGCAA 
ACTAAAGACA CTGTTACATA TAGAGAAGGC CACATAAACG CACAGGATGA ACAGCAAATG 
AAAGACTCCA TTACATACAG CGCAGCCAAG ATAAGTACTC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCG TCTTGGATAC 
TTTGGAGCAA GTCTGTATGG GACCTCTTCA CATCTGCCCT GGCTTGCTGG ATTAATTGAA 
GCTCCAAGTC TATGTAACAC TTGCTCCAGA GTCTCCATCC CAATCTCAGC ATTATGTGCC 
TGTGCCTCTT CTGGATCCCA TATGTCCCAT TGGGATAGCA GTCATAAGGA GAACAATCAA 
ACTAAAGACA TGATTACATA CAGTGAAGTT CAGACAAGTA TGCAGGATGA ACAACAAGCT 
AAAGGCACTA TTACATACAG TGATGGTCAG GTAAAAACTC ATGTGTTTGC AGAACAAAAG 
TCCAATCACC AAGGCACCAG GAGTATCAGA CAAGAAGGTG GTCAGATGTC AGCGCTGATC 
AAGAATAGCG AAATATCTGA AGACAATATT ACAGATGATA TAAGATCTCT GTTAAGGCCT 
CATCCTGAGT GTCCAGTTCA GTTTTCGGGC CCAGCCCTTA TAAAGGATGT TAGTGAGCTG 
GAGAGAGTGC AGTGCCATTC AACTAAACTG GTAAAAGAGA TGGGAGAATT TAGCTATGAG 
GAAAGACAAT CAAGTGCAAG CTTTCCCCTG CAGCTCTCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACCAG CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746988.1
CDS	441..9935
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891128.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891128.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCTGA TGAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGGCACTATT 
ACATACACTG AAGCGCAGAG ACATTCTCAG AATTTAGAAA ATGTTGACCA GTCTGCATCT 
AACAAGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TTGGATACTC TGGGTCAGGT 
CTATATGGCG CCTCTTTACA TCAGACCAGA TCTGCAGGAT TATCCGAAGC TACAACTCTA 
TACAAAAATG TCTCTGGAGT CTCCATCTCA GCCTCTGCAT CATCTGCATC ATCCTCTTCT 
GGATACCTTG TATCCCCAAT GGAAAGCAGT CTTAAGAATT TAGAAAGTGT TGGTCAGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GAATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTATC CGAAGCTACA 
ACTCTATACA AAAATGTCTC TGGAGTCTCC ATCTCAGCCT CTGCATCATC TGCATCGTCC 
TCTTCTGGAT ACCTTGCATC CCCAATGGAA AGCAGTGTTA AGAATTTAGA AAATGTTGGT 
CAGTCTGCTT CTAACAAGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTTGGATAC 
TCTGGGTCAG GTCTATATGG CGCCTCTTTA CATCAGACCA GATCTGCAGG ATTATCCGAA 
GCTACAACTC TATACAAAAA TGTCTCTGGA GTCTCCATCC CAGCCTCTGC ATCGTCCTCT 
TCTGGATACC TTGCATCCCT AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACTAAAGAC 
ACTATTACAT ACACTGAAGC GCAGAGAAAT TCTCAGGATA AAGAACAAAC TAAAGAAACT 
ATTACATACA GGGAAAGCCA GATAAATACT CAGAATTTAG AAAATGTTGG CCGGTCTGCC 
TCTAACAAGC AAAATGGGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTGTCCGA AGCTACAACT 
CTATACAAAA ATGTCTCAGG AGGCTCCATC TCAGCCTCTG CATCATCTGC ATTGTCCTCT 
TCTGGATACC TTGCATCCTC AATGGAAAGC AGTGTTAAGG ATAAAGAGCA AACCAAAGAC 
ACTTTTACAT ACATTGAAGC TCAGATAAAC TCTCAGGGCA AAGAGCAAAC TAAAGATATT 
ATTACATACA GTGAAGCTCA GAGACATTTT CAGGATAAAG AGCAACCTAA AGAGAGTATT 
ACATGTAGTG AAGTTTATAG AAGTACTCAG GATAAAGAGC AAACTAAAGA CACAATTACA 
TACAGTAAAG TTCAGAGAAA TATGCAGAAT CTAGAAAATG TTGGTCGGTC TGCCCCTAAC 
AGGCAAAATG TGATGAGCAG TGTATCTGGG GATCATCTAG GAAACACTGG ACCAAGTCTG 
TATGGGGCTT CTTTAGCAGG ATTATCCGAA GCTCCAAGCT TTCCATCATC CATCTCTTCC 
CCTCCTGTAT ACCCTGTGTC TCCAACAGAA AACAGTCAAC GGGTAACCTC AGCAGCAATT 
GATAAAAAGC AAACTAAAGA CTCTATTACC TACAGTGTTG GTCAGATGAA TACTCAGGAT 
AAAAAGCAAA CTCTATTAAC CTACAGTGTT GGTCAGATAA AGTCTCAGGA TATAAAGCAA 
ACTAAAGACA CTGTTACATA TAGAGAAGGC CACATAAACG CACAGGATGA ACAGCAAATG 
AAAGACTCCA TTACATACAG CGCAGCCAAG ATAAGTACTC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCG TCTTGGATAC 
TTTGGAGCAA GTCTGTATGG GACCTCTTCA CATCTGCCCT GGCTTGCTGG ATTAATTGAA 
GCTCCAAGTC TATGTAACAC TTGCTCCAGA GTCTCCATCC CAATCTCAGC ATTATGTGCC 
TGTGCCTCTT CTGGATCCCA TATGTCCCAT TGGGATAGCA GTCATAAGGA GAACAATCAA 
ACTAAAGACA TGATTACATA CAGTGAAGTT CAGACAAGTA TGCAGGATGA ACAACAAGCT 
AAAGGCACTA TTACATACAG TGATGGTCAG GTAAAAACTC ATGTGTTTGC AGAACAAAAG 
TCCAATCACC AAGGCACCAG GAGTATCAGA CAAGAAGGTG GTCAGATGTC AGCGCTGATC 
AAGAATAGCG AAATATCTGA AGACAATATT ACAGATGATA TAAGATCTCT GTTAAGGCCT 
CATCCTGAGT GTCCAGTTCA GTTTTCGGGC CCAGCCCTTA TAAAGGATGT TAGTGAGCTG 
GAGAGAGTGC AGTGCCATTC AACTAAACTG GTAAAAGAGA TGGGAGAATT TAGCTATGAG 
GAAAGACAAT CAAGTGCAAG CTTTCCCCTG CAGCTCTCAT TCAAACAGGG AGTTTCCAAA 
GACTTGGAGG ACATCACTTC AACCTATGTC TATAGCCCTG GGATTCATAC TGCATGCATA 
GGCAATAACA ATGTTCTGAA GTCTGTGTAT ACGTATAAAG GTTTGCAAAC TGACAGCCTT 
CATGCTGATA TCATGAATGG TAATGAGTGC AAATGCACAG AAGAGACCAG CAATCATCTT 
AATGACGGCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891129.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746989.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X22, mRNA.

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CloneID	OXa64396
Clone ID Related Accession (Same CDS sequence)	XM_031891129.1
Accession Version	XM_031891129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ATACAGCCGG AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ACACGACGAA AGATACATAA AGAGATAGAA 
CCAAATATAC AAACAGAGAT AGAAAAAGAA ATGCAACCAG ACATACGACC AGAAATACAT 
ACAGAGATAG AAAAAGATAT TCAACCAGAG ATACAATCAG AAACACCGAG AGAGGCACAA 
ATAAAAGGAC CGAAAAAAGA TTCTCGTATA CTGACAGTAA TAATTGCAGT TTCGATACTG 
TGCGGAATAT TTGTTGGTAT AGCTCTTTAT TATGGAATAA GACGACGACT AAAGGTTAAA 
AATCAGTCTA ATTGCACTAT TACATACAGT GAAGTTCTGA CAAGTACTCA GGATAAAGAG 
CATATTAAAG ACTCTATTAC ATACAGTGAA GTTCAGATAA ATACTCAGGA TAAAGTGCAT 
ACTAAAGACA CTATTACATA CAGTGACGTT CAGATAAATT CTAGGAGAAA TATGCAGGGC 
AAAGAAGAAC CTGATTATAA TGATTATACT ATTACATTCA GTGAAGGTCA GAGACATACA 
CAGAATAAAA AACAGTCTGA TGATGCTATT ACATACAGTA AAGTTCAGAT AAATACTCAG 
GATAAAGAGC ATATTAAAGA CTCTATTACT TACAGTGACG TTCAGATAAA TACTCAGGAT 
AAAGTGCATA CTAAAGACAC TATTACATAC AGTGACGTTC TGATGAATTC TAGGAGAAAT 
ATGCAGGATA AAGAGCAAAC TAAAGGCACT ATTACATACA CTGAAGCGCA GAGACATTCT 
CAGAATTTAG AAAATGTTGA CCAGTCTGCA TCTAACAAGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTTGGATA CTCTGGGTCA GGTCTATATG GCGCCTCTTT ACATCAGACC 
AGATCTGCAG GATTATCCGA AGCTACAACT CTATACAAAA ATGTCTCTGG AGTCTCCATC 
TCAGCCTCTG CATCATCTGC ATCATCCTCT TCTGGATACC TTGTATCCCC AATGGAAAGC 
AGTCTTAAGA ATTTAGAAAG TGTTGGTCAG TCTGCCTCTA ACAAGCAAAA TGGGATGAGC 
AGTGAATCTG GGGATCATCT TGGATACTCT GGGTCAGGTC TATATGGCGC CTCTTTACAT 
CAGACCAGAT CTGCAGGATT ATCCGAAGCT ACAACTCTAT ACAAAAATGT CTCTGGAGTC 
TCCATCTCAG CCTCTGCATC ATCTGCATCG TCCTCTTCTG GATACCTTGC ATCCCCAATG 
GAAAGCAGTG TTAAGAATTT AGAAAATGTT GGTCAGTCTG CTTCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCCCAGCCTC TGCATCGTCC TCTTCTGGAT ACCTTGCATC CCTAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACTAAA GACACTATTA CATACACTGA AGCGCAGAGA 
AATTCTCAGG ATAAAGAACA AACTAAAGAA ACTATTACAT ACAGGGAAAG CCAGATAAAT 
ACTCAGAATT TAGAAAATGT TGGCCGGTCT GCCTCTAACA AGCAAAATGG GATGAGCAGT 
GTATCTGGGG ATCATCTTGG ATACTCTGGG TCAGGTCTAT ATGGCGCCTC TTTACATCAG 
ACCAGATCTG CAGGATTGTC CGAAGCTACA ACTCTATACA AAAATGTCTC AGGAGGCTCC 
ATCTCAGCCT CTGCATCATC TGCATTGTCC TCTTCTGGAT ACCTTGCATC CTCAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACCAAA GACACTTTTA CATACATTGA AGCTCAGATA 
AACTCTCAGG GCAAAGAGCA AACTAAAGAT ATTATTACAT ACAGTGAAGC TCAGAGACAT 
TTTCAGGATA AAGAGCAACC TAAAGAGAGT ATTACATGTA GTGAAGTTTA TAGAAGTACT 
CAGGATAAAG AGCAAACTAA AGACACAATT ACATACAGTA AAGTTCAGAG AAATATGCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCATC TAGGAAACAC TGGACCAAGT CTGTATGGGG CTTCTTTAGC AGGATTATCC 
GAAGCTCCAA GCTTTCCATC ATCCATCTCT TCCCCTCCTG TATACCCTGT GTCTCCAACA 
GAAAACAGTC AACGGGTAAC CTCAGCAGCA ATTGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCACAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGCGCAGCC 
AAGATAAGTA CTCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACCTCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGGAGTATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCT CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC CAGCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746989.1
CDS	441..9908
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891129.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891129.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ATACAGCCGG AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAAAAAGAG ACACGACGAA AGATACATAA AGAGATAGAA 
CCAAATATAC AAACAGAGAT AGAAAAAGAA ATGCAACCAG ACATACGACC AGAAATACAT 
ACAGAGATAG AAAAAGATAT TCAACCAGAG ATACAATCAG AAACACCGAG AGAGGCACAA 
ATAAAAGGAC CGAAAAAAGA TTCTCGTATA CTGACAGTAA TAATTGCAGT TTCGATACTG 
TGCGGAATAT TTGTTGGTAT AGCTCTTTAT TATGGAATAA GACGACGACT AAAGGTTAAA 
AATCAGTCTA ATTGCACTAT TACATACAGT GAAGTTCTGA CAAGTACTCA GGATAAAGAG 
CATATTAAAG ACTCTATTAC ATACAGTGAA GTTCAGATAA ATACTCAGGA TAAAGTGCAT 
ACTAAAGACA CTATTACATA CAGTGACGTT CAGATAAATT CTAGGAGAAA TATGCAGGGC 
AAAGAAGAAC CTGATTATAA TGATTATACT ATTACATTCA GTGAAGGTCA GAGACATACA 
CAGAATAAAA AACAGTCTGA TGATGCTATT ACATACAGTA AAGTTCAGAT AAATACTCAG 
GATAAAGAGC ATATTAAAGA CTCTATTACT TACAGTGACG TTCAGATAAA TACTCAGGAT 
AAAGTGCATA CTAAAGACAC TATTACATAC AGTGACGTTC TGATGAATTC TAGGAGAAAT 
ATGCAGGATA AAGAGCAAAC TAAAGGCACT ATTACATACA CTGAAGCGCA GAGACATTCT 
CAGAATTTAG AAAATGTTGA CCAGTCTGCA TCTAACAAGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTTGGATA CTCTGGGTCA GGTCTATATG GCGCCTCTTT ACATCAGACC 
AGATCTGCAG GATTATCCGA AGCTACAACT CTATACAAAA ATGTCTCTGG AGTCTCCATC 
TCAGCCTCTG CATCATCTGC ATCATCCTCT TCTGGATACC TTGTATCCCC AATGGAAAGC 
AGTCTTAAGA ATTTAGAAAG TGTTGGTCAG TCTGCCTCTA ACAAGCAAAA TGGGATGAGC 
AGTGAATCTG GGGATCATCT TGGATACTCT GGGTCAGGTC TATATGGCGC CTCTTTACAT 
CAGACCAGAT CTGCAGGATT ATCCGAAGCT ACAACTCTAT ACAAAAATGT CTCTGGAGTC 
TCCATCTCAG CCTCTGCATC ATCTGCATCG TCCTCTTCTG GATACCTTGC ATCCCCAATG 
GAAAGCAGTG TTAAGAATTT AGAAAATGTT GGTCAGTCTG CTTCTAACAA GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCATCTTGGA TACTCTGGGT CAGGTCTATA TGGCGCCTCT 
TTACATCAGA CCAGATCTGC AGGATTATCC GAAGCTACAA CTCTATACAA AAATGTCTCT 
GGAGTCTCCA TCCCAGCCTC TGCATCGTCC TCTTCTGGAT ACCTTGCATC CCTAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACTAAA GACACTATTA CATACACTGA AGCGCAGAGA 
AATTCTCAGG ATAAAGAACA AACTAAAGAA ACTATTACAT ACAGGGAAAG CCAGATAAAT 
ACTCAGAATT TAGAAAATGT TGGCCGGTCT GCCTCTAACA AGCAAAATGG GATGAGCAGT 
GTATCTGGGG ATCATCTTGG ATACTCTGGG TCAGGTCTAT ATGGCGCCTC TTTACATCAG 
ACCAGATCTG CAGGATTGTC CGAAGCTACA ACTCTATACA AAAATGTCTC AGGAGGCTCC 
ATCTCAGCCT CTGCATCATC TGCATTGTCC TCTTCTGGAT ACCTTGCATC CTCAATGGAA 
AGCAGTGTTA AGGATAAAGA GCAAACCAAA GACACTTTTA CATACATTGA AGCTCAGATA 
AACTCTCAGG GCAAAGAGCA AACTAAAGAT ATTATTACAT ACAGTGAAGC TCAGAGACAT 
TTTCAGGATA AAGAGCAACC TAAAGAGAGT ATTACATGTA GTGAAGTTTA TAGAAGTACT 
CAGGATAAAG AGCAAACTAA AGACACAATT ACATACAGTA AAGTTCAGAG AAATATGCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCATC TAGGAAACAC TGGACCAAGT CTGTATGGGG CTTCTTTAGC AGGATTATCC 
GAAGCTCCAA GCTTTCCATC ATCCATCTCT TCCCCTCCTG TATACCCTGT GTCTCCAACA 
GAAAACAGTC AACGGGTAAC CTCAGCAGCA ATTGATAAAA AGCAAACTAA AGACTCTATT 
ACCTACAGTG TTGGTCAGAT GAATACTCAG GATAAAAAGC AAACTCTATT AACCTACAGT 
GTTGGTCAGA TAAAGTCTCA GGATATAAAG CAAACTAAAG ACACTGTTAC ATATAGAGAA 
GGCCACATAA ACGCACAGGA TGAACAGCAA ATGAAAGACT CCATTACATA CAGCGCAGCC 
AAGATAAGTA CTCAGAATCT AGAAAATGTT GGTCGGTCTG CCCCTAACAG GCAAAATGTG 
ATGAGCAGTG TATCTGGGGA TCGTCTTGGA TACTTTGGAG CAAGTCTGTA TGGGACCTCT 
TCACATCTGC CCTGGCTTGC TGGATTAATT GAAGCTCCAA GTCTATGTAA CACTTGCTCC 
AGAGTCTCCA TCCCAATCTC AGCATTATGT GCCTGTGCCT CTTCTGGATC CCATATGTCC 
CATTGGGATA GCAGTCATAA GGAGAACAAT CAAACTAAAG ACATGATTAC ATACAGTGAA 
GTTCAGACAA GTATGCAGGA TGAACAACAA GCTAAAGGCA CTATTACATA CAGTGATGGT 
CAGGTAAAAA CTCATGTGTT TGCAGAACAA AAGTCCAATC ACCAAGGCAC CAGGAGTATC 
AGACAAGAAG GTGGTCAGAT GTCAGCGCTG ATCAAGAATA GCGAAATATC TGAAGACAAT 
ATTACAGATG ATATAAGATC TCTGTTAAGG CCTCATCCTG AGTGTCCAGT TCAGTTTTCG 
GGCCCAGCCC TTATAAAGGA TGTTAGTGAG CTGGAGAGAG TGCAGTGCCA TTCAACTAAA 
CTGGTAAAAG AGATGGGAGA ATTTAGCTAT GAGGAAAGAC AATCAAGTGC AAGCTTTCCC 
CTGCAGCTCT CATTCAAACA GGGAGTTTCC AAAGACTTGG AGGACATCAC TTCAACCTAT 
GTCTATAGCC CTGGGATTCA TACTGCATGC ATAGGCAATA ACAATGTTCT GAAGTCTGTG 
TATACGTATA AAGGTTTGCA AACTGACAGC CTTCATGCTG ATATCATGAA TGGTAATGAG 
TGCAAATGCA CAGAAGAGAC CAGCAATCAT CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891130.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746990.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X23, mRNA.

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CloneID	OXa64397
Clone ID Related Accession (Same CDS sequence)	XM_031891130.1
Accession Version	XM_031891130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAAAGAGAC ACGACGAAAG ATACATAAAG AGATAGAACC AAATATACAA 
ACAGAGATAG AAAAAGAAAT GCAACCAGAC ATACGACCAG AAATACATAC AGAGATAGAA 
AAAGATATTC AACCAGAGAT ACAATCAGAA ACACCGAGAG AGGCACAAAT AAAAGGACCG 
AAAAAAGATT CTCGTATACT GACAGTAATA ATTGCAGTTT CGATACTGTG CGGAATATTT 
GTTGGTATAG CTCTTTATTA TGGAATAAGA CGACGACTAA AGGTTAAAAA TCAGTCTAAT 
TGCACTATTA CATACAGTGA AGTTCTGACA AGTACTCAGG ATAAAGAGCA TATTAAAGAC 
TCTATTACAT ACAGTGAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGACGTTCA GATAAATTCT AGGAGAAATA TGCAGGGCAA AGAAGAACCT 
GATTATAATG ATTATACTAT TACATTCAGT GAAGGTCAGA GACATACACA GAATAAAAAA 
CAGTCTGATG ATGCTATTAC ATACAGTAAA GTTCAGATAA ATACTCAGGA TAAAGAGCAT 
ATTAAAGACT CTATTACTTA CAGTGACGTT CAGATAAATA CTCAGGATAA AGTGCATACT 
AAAGACACTA TTACATACAG TGACGTTCTG ATGAATTCTA GGAGAAATAT GCAGGATAAA 
GAGCAAACTA AAGGCACTAT TACATACACT GAAGCGCAGA GACATTCTCA GAATTTAGAA 
AATGTTGACC AGTCTGCATC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCTC AGCCTCTGCA 
TCATCTGCAT CATCCTCTTC TGGATACCTT GTATCCCCAA TGGAAAGCAG TCTTAAGAAT 
TTAGAAAGTG TTGGTCAGTC TGCCTCTAAC AAGCAAAATG GGATGAGCAG TGAATCTGGG 
GATCATCTTG GATACTCTGG GTCAGGTCTA TATGGCGCCT CTTTACATCA GACCAGATCT 
GCAGGATTAT CCGAAGCTAC AACTCTATAC AAAAATGTCT CTGGAGTCTC CATCTCAGCC 
TCTGCATCAT CTGCATCGTC CTCTTCTGGA TACCTTGCAT CCCCAATGGA AAGCAGTGTT 
AAGAATTTAG AAAATGTTGG TCAGTCTGCT TCTAACAAGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTTGGATA CTCTGGGTCA GGTCTATATG GCGCCTCTTT ACATCAGACC 
AGATCTGCAG GATTATCCGA AGCTACAACT CTATACAAAA ATGTCTCTGG AGTCTCCATC 
CCAGCCTCTG CATCGTCCTC TTCTGGATAC CTTGCATCCC TAATGGAAAG CAGTGTTAAG 
GATAAAGAGC AAACTAAAGA CACTATTACA TACACTGAAG CGCAGAGAAA TTCTCAGGAT 
AAAGAACAAA CTAAAGAAAC TATTACATAC AGGGAAAGCC AGATAAATAC TCAGAATTTA 
GAAAATGTTG GCCGGTCTGC CTCTAACAAG CAAAATGGGA TGAGCAGTGT ATCTGGGGAT 
CATCTTGGAT ACTCTGGGTC AGGTCTATAT GGCGCCTCTT TACATCAGAC CAGATCTGCA 
GGATTGTCCG AAGCTACAAC TCTATACAAA AATGTCTCAG GAGGCTCCAT CTCAGCCTCT 
GCATCATCTG CATTGTCCTC TTCTGGATAC CTTGCATCCT CAATGGAAAG CAGTGTTAAG 
GATAAAGAGC AAACCAAAGA CACTTTTACA TACATTGAAG CTCAGATAAA CTCTCAGGGC 
AAAGAGCAAA CTAAAGATAT TATTACATAC AGTGAAGCTC AGAGACATTT TCAGGATAAA 
GAGCAACCTA AAGAGAGTAT TACATGTAGT GAAGTTTATA GAAGTACTCA GGATAAAGAG 
CAAACTAAAG ACACAATTAC ATACAGTAAA GTTCAGAGAA ATATGCAGAA TCTAGAAAAT 
GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTA 
GGAAACACTG GACCAAGTCT GTATGGGGCT TCTTTAGCAG GATTATCCGA AGCTCCAAGC 
TTTCCATCAT CCATCTCTTC CCCTCCTGTA TACCCTGTGT CTCCAACAGA AAACAGTCAA 
CGGGTAACCT CAGCAGCAAT TGATAAAAAG CAAACTAAAG ACTCTATTAC CTACAGTGTT 
GGTCAGATGA ATACTCAGGA TAAAAAGCAA ACTCTATTAA CCTACAGTGT TGGTCAGATA 
AAGTCTCAGG ATATAAAGCA AACTAAAGAC ACTGTTACAT ATAGAGAAGG CCACATAAAC 
GCACAGGATG AACAGCAAAT GAAAGACTCC ATTACATACA GCGCAGCCAA GATAAGTACT 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC GTCTTGGATA CTTTGGAGCA AGTCTGTATG GGACCTCTTC ACATCTGCCC 
TGGCTTGCTG GATTAATTGA AGCTCCAAGT CTATGTAACA CTTGCTCCAG AGTCTCCATC 
CCAATCTCAG CATTATGTGC CTGTGCCTCT TCTGGATCCC ATATGTCCCA TTGGGATAGC 
AGTCATAAGG AGAACAATCA AACTAAAGAC ATGATTACAT ACAGTGAAGT TCAGACAAGT 
ATGCAGGATG AACAACAAGC TAAAGGCACT ATTACATACA GTGATGGTCA GGTAAAAACT 
CATGTGTTTG CAGAACAAAA GTCCAATCAC CAAGGCACCA GGAGTATCAG ACAAGAAGGT 
GGTCAGATGT CAGCGCTGAT CAAGAATAGC GAAATATCTG AAGACAATAT TACAGATGAT 
ATAAGATCTC TGTTAAGGCC TCATCCTGAG TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT 
ATAAAGGATG TTAGTGAGCT GGAGAGAGTG CAGTGCCATT CAACTAAACT GGTAAAAGAG 
ATGGGAGAAT TTAGCTATGA GGAAAGACAA TCAAGTGCAA GCTTTCCCCT GCAGCTCTCA 
TTCAAACAGG GAGTTTCCAA AGACTTGGAG GACATCACTT CAACCTATGT CTATAGCCCT 
GGGATTCATA CTGCATGCAT AGGCAATAAC AATGTTCTGA AGTCTGTGTA TACGTATAAA 
GGTTTGCAAA CTGACAGCCT TCATGCTGAT ATCATGAATG GTAATGAGTG CAAATGCACA 
GAAGAGACCA GCAATCATCT TAATGACGGC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746990.1
CDS	441..9896
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891130.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891130.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAAAGAGAT ACAGCCGGAG ATACAAACAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAAAGAGAC ACGACGAAAG ATACATAAAG AGATAGAACC AAATATACAA 
ACAGAGATAG AAAAAGAAAT GCAACCAGAC ATACGACCAG AAATACATAC AGAGATAGAA 
AAAGATATTC AACCAGAGAT ACAATCAGAA ACACCGAGAG AGGCACAAAT AAAAGGACCG 
AAAAAAGATT CTCGTATACT GACAGTAATA ATTGCAGTTT CGATACTGTG CGGAATATTT 
GTTGGTATAG CTCTTTATTA TGGAATAAGA CGACGACTAA AGGTTAAAAA TCAGTCTAAT 
TGCACTATTA CATACAGTGA AGTTCTGACA AGTACTCAGG ATAAAGAGCA TATTAAAGAC 
TCTATTACAT ACAGTGAAGT TCAGATAAAT ACTCAGGATA AAGTGCATAC TAAAGACACT 
ATTACATACA GTGACGTTCA GATAAATTCT AGGAGAAATA TGCAGGGCAA AGAAGAACCT 
GATTATAATG ATTATACTAT TACATTCAGT GAAGGTCAGA GACATACACA GAATAAAAAA 
CAGTCTGATG ATGCTATTAC ATACAGTAAA GTTCAGATAA ATACTCAGGA TAAAGAGCAT 
ATTAAAGACT CTATTACTTA CAGTGACGTT CAGATAAATA CTCAGGATAA AGTGCATACT 
AAAGACACTA TTACATACAG TGACGTTCTG ATGAATTCTA GGAGAAATAT GCAGGATAAA 
GAGCAAACTA AAGGCACTAT TACATACACT GAAGCGCAGA GACATTCTCA GAATTTAGAA 
AATGTTGACC AGTCTGCATC TAACAAGCAA AATGTGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTATCCGAAG CTACAACTCT ATACAAAAAT GTCTCTGGAG TCTCCATCTC AGCCTCTGCA 
TCATCTGCAT CATCCTCTTC TGGATACCTT GTATCCCCAA TGGAAAGCAG TCTTAAGAAT 
TTAGAAAGTG TTGGTCAGTC TGCCTCTAAC AAGCAAAATG GGATGAGCAG TGAATCTGGG 
GATCATCTTG GATACTCTGG GTCAGGTCTA TATGGCGCCT CTTTACATCA GACCAGATCT 
GCAGGATTAT CCGAAGCTAC AACTCTATAC AAAAATGTCT CTGGAGTCTC CATCTCAGCC 
TCTGCATCAT CTGCATCGTC CTCTTCTGGA TACCTTGCAT CCCCAATGGA AAGCAGTGTT 
AAGAATTTAG AAAATGTTGG TCAGTCTGCT TCTAACAAGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC ATCTTGGATA CTCTGGGTCA GGTCTATATG GCGCCTCTTT ACATCAGACC 
AGATCTGCAG GATTATCCGA AGCTACAACT CTATACAAAA ATGTCTCTGG AGTCTCCATC 
CCAGCCTCTG CATCGTCCTC TTCTGGATAC CTTGCATCCC TAATGGAAAG CAGTGTTAAG 
GATAAAGAGC AAACTAAAGA CACTATTACA TACACTGAAG CGCAGAGAAA TTCTCAGGAT 
AAAGAACAAA CTAAAGAAAC TATTACATAC AGGGAAAGCC AGATAAATAC TCAGAATTTA 
GAAAATGTTG GCCGGTCTGC CTCTAACAAG CAAAATGGGA TGAGCAGTGT ATCTGGGGAT 
CATCTTGGAT ACTCTGGGTC AGGTCTATAT GGCGCCTCTT TACATCAGAC CAGATCTGCA 
GGATTGTCCG AAGCTACAAC TCTATACAAA AATGTCTCAG GAGGCTCCAT CTCAGCCTCT 
GCATCATCTG CATTGTCCTC TTCTGGATAC CTTGCATCCT CAATGGAAAG CAGTGTTAAG 
GATAAAGAGC AAACCAAAGA CACTTTTACA TACATTGAAG CTCAGATAAA CTCTCAGGGC 
AAAGAGCAAA CTAAAGATAT TATTACATAC AGTGAAGCTC AGAGACATTT TCAGGATAAA 
GAGCAACCTA AAGAGAGTAT TACATGTAGT GAAGTTTATA GAAGTACTCA GGATAAAGAG 
CAAACTAAAG ACACAATTAC ATACAGTAAA GTTCAGAGAA ATATGCAGAA TCTAGAAAAT 
GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT GTGATGAGCA GTGTATCTGG GGATCATCTA 
GGAAACACTG GACCAAGTCT GTATGGGGCT TCTTTAGCAG GATTATCCGA AGCTCCAAGC 
TTTCCATCAT CCATCTCTTC CCCTCCTGTA TACCCTGTGT CTCCAACAGA AAACAGTCAA 
CGGGTAACCT CAGCAGCAAT TGATAAAAAG CAAACTAAAG ACTCTATTAC CTACAGTGTT 
GGTCAGATGA ATACTCAGGA TAAAAAGCAA ACTCTATTAA CCTACAGTGT TGGTCAGATA 
AAGTCTCAGG ATATAAAGCA AACTAAAGAC ACTGTTACAT ATAGAGAAGG CCACATAAAC 
GCACAGGATG AACAGCAAAT GAAAGACTCC ATTACATACA GCGCAGCCAA GATAAGTACT 
CAGAATCTAG AAAATGTTGG TCGGTCTGCC CCTAACAGGC AAAATGTGAT GAGCAGTGTA 
TCTGGGGATC GTCTTGGATA CTTTGGAGCA AGTCTGTATG GGACCTCTTC ACATCTGCCC 
TGGCTTGCTG GATTAATTGA AGCTCCAAGT CTATGTAACA CTTGCTCCAG AGTCTCCATC 
CCAATCTCAG CATTATGTGC CTGTGCCTCT TCTGGATCCC ATATGTCCCA TTGGGATAGC 
AGTCATAAGG AGAACAATCA AACTAAAGAC ATGATTACAT ACAGTGAAGT TCAGACAAGT 
ATGCAGGATG AACAACAAGC TAAAGGCACT ATTACATACA GTGATGGTCA GGTAAAAACT 
CATGTGTTTG CAGAACAAAA GTCCAATCAC CAAGGCACCA GGAGTATCAG ACAAGAAGGT 
GGTCAGATGT CAGCGCTGAT CAAGAATAGC GAAATATCTG AAGACAATAT TACAGATGAT 
ATAAGATCTC TGTTAAGGCC TCATCCTGAG TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT 
ATAAAGGATG TTAGTGAGCT GGAGAGAGTG CAGTGCCATT CAACTAAACT GGTAAAAGAG 
ATGGGAGAAT TTAGCTATGA GGAAAGACAA TCAAGTGCAA GCTTTCCCCT GCAGCTCTCA 
TTCAAACAGG GAGTTTCCAA AGACTTGGAG GACATCACTT CAACCTATGT CTATAGCCCT 
GGGATTCATA CTGCATGCAT AGGCAATAAC AATGTTCTGA AGTCTGTGTA TACGTATAAA 
GGTTTGCAAA CTGACAGCCT TCATGCTGAT ATCATGAATG GTAATGAGTG CAAATGCACA 
GAAGAGACCA GCAATCATCT TAATGACGGC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891131.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746991.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X24, mRNA.

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CloneID	OXa64398
Clone ID Related Accession (Same CDS sequence)	XM_031891131.1
Accession Version	XM_031891131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GGATAAAGAA 
CAAACTAAAG AAACTATTAC ATACAGGGAA AGCCAGATAA ATACTCAGAA TTTAGAAAAT 
GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTG 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCAGGAGGCT CCATCTCAGC CTCTGCATCA 
TCTGCATTGT CCTCTTCTGG ATACCTTGCA TCCTCAATGG AAAGCAGTGT TAAGGATAAA 
GAGCAAACCA AAGACACTTT TACATACATT GAAGCTCAGA TAAACTCTCA GGGCAAAGAG 
CAAACTAAAG ATATTATTAC ATACAGTGAA GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA 
CCTAAAGAGA GTATTACATG TAGTGAAGTT TATAGAAGTA CTCAGGATAA AGAGCAAACT 
AAAGACACAA TTACATACAG TAAAGTTCAG AGAAATATGC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTAGGAAAC 
ACTGGACCAA GTCTGTATGG GGCTTCTTTA GCAGGATTAT CCGAAGCTCC AAGCTTTCCA 
TCATCCATCT CTTCCCCTCC TGTATACCCT GTGTCTCCAA CAGAAAACAG TCAACGGGTA 
ACCTCAGCAG CAATTGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCACAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGCGCAG CCAAGATAAG TACTCAGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACCT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGGAGTA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CTCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746991.1
CDS	441..9890
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891131.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891131.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GGATAAAGAA 
CAAACTAAAG AAACTATTAC ATACAGGGAA AGCCAGATAA ATACTCAGAA TTTAGAAAAT 
GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTG 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCAGGAGGCT CCATCTCAGC CTCTGCATCA 
TCTGCATTGT CCTCTTCTGG ATACCTTGCA TCCTCAATGG AAAGCAGTGT TAAGGATAAA 
GAGCAAACCA AAGACACTTT TACATACATT GAAGCTCAGA TAAACTCTCA GGGCAAAGAG 
CAAACTAAAG ATATTATTAC ATACAGTGAA GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA 
CCTAAAGAGA GTATTACATG TAGTGAAGTT TATAGAAGTA CTCAGGATAA AGAGCAAACT 
AAAGACACAA TTACATACAG TAAAGTTCAG AGAAATATGC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTAGGAAAC 
ACTGGACCAA GTCTGTATGG GGCTTCTTTA GCAGGATTAT CCGAAGCTCC AAGCTTTCCA 
TCATCCATCT CTTCCCCTCC TGTATACCCT GTGTCTCCAA CAGAAAACAG TCAACGGGTA 
ACCTCAGCAG CAATTGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCACAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGCGCAG CCAAGATAAG TACTCAGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACCT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGGAGTA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CTCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891132.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746992.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X25, mRNA.

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CloneID	OXa64399
Clone ID Related Accession (Same CDS sequence)	XM_031891132.1
Accession Version	XM_031891132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACTAAAG ACACTATTAC ATACACTGAA GCGCAGAGAA ATTCTCAGAA TTTAGAAAAT 
GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTG 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCAGGAGGCT CCATCTCAGC CTCTGCATCA 
TCTGCATTGT CCTCTTCTGG ATACCTTGCA TCCTCAATGG AAAGCAGTGT TAAGGATAAA 
GAGCAAACCA AAGACACTTT TACATACATT GAAGCTCAGA TAAACTCTCA GGGCAAAGAG 
CAAACTAAAG ATATTATTAC ATACAGTGAA GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA 
CCTAAAGAGA GTATTACATG TAGTGAAGTT TATAGAAGTA CTCAGGATAA AGAGCAAACT 
AAAGACACAA TTACATACAG TAAAGTTCAG AGAAATATGC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTAGGAAAC 
ACTGGACCAA GTCTGTATGG GGCTTCTTTA GCAGGATTAT CCGAAGCTCC AAGCTTTCCA 
TCATCCATCT CTTCCCCTCC TGTATACCCT GTGTCTCCAA CAGAAAACAG TCAACGGGTA 
ACCTCAGCAG CAATTGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCACAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGCGCAG CCAAGATAAG TACTCAGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACCT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGGAGTA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CTCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746992.1
CDS	441..9890
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKDKEQTKDTITYTEAQRNSQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891132.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891132.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACTAAAG ACACTATTAC ATACACTGAA GCGCAGAGAA ATTCTCAGAA TTTAGAAAAT 
GTTGGCCGGT CTGCCTCTAA CAAGCAAAAT GGGATGAGCA GTGTATCTGG GGATCATCTT 
GGATACTCTG GGTCAGGTCT ATATGGCGCC TCTTTACATC AGACCAGATC TGCAGGATTG 
TCCGAAGCTA CAACTCTATA CAAAAATGTC TCAGGAGGCT CCATCTCAGC CTCTGCATCA 
TCTGCATTGT CCTCTTCTGG ATACCTTGCA TCCTCAATGG AAAGCAGTGT TAAGGATAAA 
GAGCAAACCA AAGACACTTT TACATACATT GAAGCTCAGA TAAACTCTCA GGGCAAAGAG 
CAAACTAAAG ATATTATTAC ATACAGTGAA GCTCAGAGAC ATTTTCAGGA TAAAGAGCAA 
CCTAAAGAGA GTATTACATG TAGTGAAGTT TATAGAAGTA CTCAGGATAA AGAGCAAACT 
AAAGACACAA TTACATACAG TAAAGTTCAG AGAAATATGC AGAATCTAGA AAATGTTGGT 
CGGTCTGCCC CTAACAGGCA AAATGTGATG AGCAGTGTAT CTGGGGATCA TCTAGGAAAC 
ACTGGACCAA GTCTGTATGG GGCTTCTTTA GCAGGATTAT CCGAAGCTCC AAGCTTTCCA 
TCATCCATCT CTTCCCCTCC TGTATACCCT GTGTCTCCAA CAGAAAACAG TCAACGGGTA 
ACCTCAGCAG CAATTGATAA AAAGCAAACT AAAGACTCTA TTACCTACAG TGTTGGTCAG 
ATGAATACTC AGGATAAAAA GCAAACTCTA TTAACCTACA GTGTTGGTCA GATAAAGTCT 
CAGGATATAA AGCAAACTAA AGACACTGTT ACATATAGAG AAGGCCACAT AAACGCACAG 
GATGAACAGC AAATGAAAGA CTCCATTACA TACAGCGCAG CCAAGATAAG TACTCAGAAT 
CTAGAAAATG TTGGTCGGTC TGCCCCTAAC AGGCAAAATG TGATGAGCAG TGTATCTGGG 
GATCGTCTTG GATACTTTGG AGCAAGTCTG TATGGGACCT CTTCACATCT GCCCTGGCTT 
GCTGGATTAA TTGAAGCTCC AAGTCTATGT AACACTTGCT CCAGAGTCTC CATCCCAATC 
TCAGCATTAT GTGCCTGTGC CTCTTCTGGA TCCCATATGT CCCATTGGGA TAGCAGTCAT 
AAGGAGAACA ATCAAACTAA AGACATGATT ACATACAGTG AAGTTCAGAC AAGTATGCAG 
GATGAACAAC AAGCTAAAGG CACTATTACA TACAGTGATG GTCAGGTAAA AACTCATGTG 
TTTGCAGAAC AAAAGTCCAA TCACCAAGGC ACCAGGAGTA TCAGACAAGA AGGTGGTCAG 
ATGTCAGCGC TGATCAAGAA TAGCGAAATA TCTGAAGACA ATATTACAGA TGATATAAGA 
TCTCTGTTAA GGCCTCATCC TGAGTGTCCA GTTCAGTTTT CGGGCCCAGC CCTTATAAAG 
GATGTTAGTG AGCTGGAGAG AGTGCAGTGC CATTCAACTA AACTGGTAAA AGAGATGGGA 
GAATTTAGCT ATGAGGAAAG ACAATCAAGT GCAAGCTTTC CCCTGCAGCT CTCATTCAAA 
CAGGGAGTTT CCAAAGACTT GGAGGACATC ACTTCAACCT ATGTCTATAG CCCTGGGATT 
CATACTGCAT GCATAGGCAA TAACAATGTT CTGAAGTCTG TGTATACGTA TAAAGGTTTG 
CAAACTGACA GCCTTCATGC TGATATCATG AATGGTAATG AGTGCAAATG CACAGAAGAG 
ACCAGCAATC ATCTTAATGA CGGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891133.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746993.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X26, mRNA.

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CloneID	OXa64400
Clone ID Related Accession (Same CDS sequence)	XM_031891133.1
Accession Version	XM_031891133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGCC GGTCTGCCTC TAACAAGCAA AATGGGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTGTCCGAAG CTACAACTCT ATACAAAAAT GTCTCAGGAG GCTCCATCTC AGCCTCTGCA 
TCATCTGCAT TGTCCTCTTC TGGATACCTT GCATCCTCAA TGGAAAGCAG TGTTAAGGAT 
AAAGAGCAAA CCAAAGACAC TTTTACATAC ATTGAAGCTC AGATAAACTC TCAGGGCAAA 
GAGCAAACTA AAGATATTAT TACATACAGT GAAGCTCAGA GACATTTTCA GGATAAAGAG 
CAACCTAAAG AGAGTATTAC ATGTAGTGAA GTTTATAGAA GTACTCAGGA TAAAGAGCAA 
ACTAAAGACA CAATTACATA CAGTAAAGTT CAGAGAAATA TGCAGAATCT AGAAAATGTT 
GGTCGGTCTG CCCCTAACAG GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTAGGA 
AACACTGGAC CAAGTCTGTA TGGGGCTTCT TTAGCAGGAT TATCCGAAGC TCCAAGCTTT 
CCATCATCCA TCTCTTCCCC TCCTGTATAC CCTGTGTCTC CAACAGAAAA CAGTCAACGG 
GTAACCTCAG CAGCAATTGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCA 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGCG CAGCCAAGAT AAGTACTCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CCTCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGGA GTATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCTCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACCAGCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746993.1
CDS	441..9833
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891133.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891133.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAGTGTT 
GGTCAGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG AATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTATCC 
GAAGCTACAA CTCTATACAA AAATGTCTCT GGAGTCTCCA TCTCAGCCTC TGCATCATCT 
GCATCGTCCT CTTCTGGATA CCTTGCATCC CCAATGGAAA GCAGTGTTAA GAATTTAGAA 
AATGTTGGCC GGTCTGCCTC TAACAAGCAA AATGGGATGA GCAGTGTATC TGGGGATCAT 
CTTGGATACT CTGGGTCAGG TCTATATGGC GCCTCTTTAC ATCAGACCAG ATCTGCAGGA 
TTGTCCGAAG CTACAACTCT ATACAAAAAT GTCTCAGGAG GCTCCATCTC AGCCTCTGCA 
TCATCTGCAT TGTCCTCTTC TGGATACCTT GCATCCTCAA TGGAAAGCAG TGTTAAGGAT 
AAAGAGCAAA CCAAAGACAC TTTTACATAC ATTGAAGCTC AGATAAACTC TCAGGGCAAA 
GAGCAAACTA AAGATATTAT TACATACAGT GAAGCTCAGA GACATTTTCA GGATAAAGAG 
CAACCTAAAG AGAGTATTAC ATGTAGTGAA GTTTATAGAA GTACTCAGGA TAAAGAGCAA 
ACTAAAGACA CAATTACATA CAGTAAAGTT CAGAGAAATA TGCAGAATCT AGAAAATGTT 
GGTCGGTCTG CCCCTAACAG GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCATCTAGGA 
AACACTGGAC CAAGTCTGTA TGGGGCTTCT TTAGCAGGAT TATCCGAAGC TCCAAGCTTT 
CCATCATCCA TCTCTTCCCC TCCTGTATAC CCTGTGTCTC CAACAGAAAA CAGTCAACGG 
GTAACCTCAG CAGCAATTGA TAAAAAGCAA ACTAAAGACT CTATTACCTA CAGTGTTGGT 
CAGATGAATA CTCAGGATAA AAAGCAAACT CTATTAACCT ACAGTGTTGG TCAGATAAAG 
TCTCAGGATA TAAAGCAAAC TAAAGACACT GTTACATATA GAGAAGGCCA CATAAACGCA 
CAGGATGAAC AGCAAATGAA AGACTCCATT ACATACAGCG CAGCCAAGAT AAGTACTCAG 
AATCTAGAAA ATGTTGGTCG GTCTGCCCCT AACAGGCAAA ATGTGATGAG CAGTGTATCT 
GGGGATCGTC TTGGATACTT TGGAGCAAGT CTGTATGGGA CCTCTTCACA TCTGCCCTGG 
CTTGCTGGAT TAATTGAAGC TCCAAGTCTA TGTAACACTT GCTCCAGAGT CTCCATCCCA 
ATCTCAGCAT TATGTGCCTG TGCCTCTTCT GGATCCCATA TGTCCCATTG GGATAGCAGT 
CATAAGGAGA ACAATCAAAC TAAAGACATG ATTACATACA GTGAAGTTCA GACAAGTATG 
CAGGATGAAC AACAAGCTAA AGGCACTATT ACATACAGTG ATGGTCAGGT AAAAACTCAT 
GTGTTTGCAG AACAAAAGTC CAATCACCAA GGCACCAGGA GTATCAGACA AGAAGGTGGT 
CAGATGTCAG CGCTGATCAA GAATAGCGAA ATATCTGAAG ACAATATTAC AGATGATATA 
AGATCTCTGT TAAGGCCTCA TCCTGAGTGT CCAGTTCAGT TTTCGGGCCC AGCCCTTATA 
AAGGATGTTA GTGAGCTGGA GAGAGTGCAG TGCCATTCAA CTAAACTGGT AAAAGAGATG 
GGAGAATTTA GCTATGAGGA AAGACAATCA AGTGCAAGCT TTCCCCTGCA GCTCTCATTC 
AAACAGGGAG TTTCCAAAGA CTTGGAGGAC ATCACTTCAA CCTATGTCTA TAGCCCTGGG 
ATTCATACTG CATGCATAGG CAATAACAAT GTTCTGAAGT CTGTGTATAC GTATAAAGGT 
TTGCAAACTG ACAGCCTTCA TGCTGATATC ATGAATGGTA ATGAGTGCAA ATGCACAGAA 
GAGACCAGCA ATCATCTTAA TGACGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891134.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746994.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X27, mRNA.

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CloneID	OXa64401
Clone ID Related Accession (Same CDS sequence)	XM_031891134.1
Accession Version	XM_031891134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746994.1
CDS	441..9587
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891134.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891134.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GGCACTATTA CATACACTGA AGCGCAGAGA CATTCTCAGA ATTTAGAAAA TGTTGACCAG 
TCTGCATCTA ACAAGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT TGGATACTCT 
GGGTCAGGTC TATATGGCGC CTCTTTACAT CAGACCAGAT CTGCAGGATT ATCCGAAGCT 
ACAACTCTAT ACAAAAATGT CTCTGGAGTC TCCATCTCAG CCTCTGCATC ATCTGCATCA 
TCCTCTTCTG GATACCTTGT ATCCCCAATG GAAAGCAGTC TTAAGAATTT AGAAAATGTT 
GGCCGGTCTG CCTCTAACAA GCAAAATGGG ATGAGCAGTG TATCTGGGGA TCATCTTGGA 
TACTCTGGGT CAGGTCTATA TGGCGCCTCT TTACATCAGA CCAGATCTGC AGGATTGTCC 
GAAGCTACAA CTCTATACAA AAATGTCTCA GGAGGCTCCA TCTCAGCCTC TGCATCATCT 
GCATTGTCCT CTTCTGGATA CCTTGCATCC TCAATGGAAA GCAGTGTTAA GGATAAAGAG 
CAAACCAAAG ACACTTTTAC ATACATTGAA GCTCAGATAA ACTCTCAGGG CAAAGAGCAA 
ACTAAAGATA TTATTACATA CAGTGAAGCT CAGAGACATT TTCAGGATAA AGAGCAACCT 
AAAGAGAGTA TTACATGTAG TGAAGTTTAT AGAAGTACTC AGGATAAAGA GCAAACTAAA 
GACACAATTA CATACAGTAA AGTTCAGAGA AATATGCAGA ATCTAGAAAA TGTTGGTCGG 
TCTGCCCCTA ACAGGCAAAA TGTGATGAGC AGTGTATCTG GGGATCATCT AGGAAACACT 
GGACCAAGTC TGTATGGGGC TTCTTTAGCA GGATTATCCG AAGCTCCAAG CTTTCCATCA 
TCCATCTCTT CCCCTCCTGT ATACCCTGTG TCTCCAACAG AAAACAGTCA ACGGGTAACC 
TCAGCAGCAA TTGATAAAAA GCAAACTAAA GACTCTATTA CCTACAGTGT TGGTCAGATG 
AATACTCAGG ATAAAAAGCA AACTCTATTA ACCTACAGTG TTGGTCAGAT AAAGTCTCAG 
GATATAAAGC AAACTAAAGA CACTGTTACA TATAGAGAAG GCCACATAAA CGCACAGGAT 
GAACAGCAAA TGAAAGACTC CATTACATAC AGCGCAGCCA AGATAAGTAC TCAGAATCTA 
GAAAATGTTG GTCGGTCTGC CCCTAACAGG CAAAATGTGA TGAGCAGTGT ATCTGGGGAT 
CGTCTTGGAT ACTTTGGAGC AAGTCTGTAT GGGACCTCTT CACATCTGCC CTGGCTTGCT 
GGATTAATTG AAGCTCCAAG TCTATGTAAC ACTTGCTCCA GAGTCTCCAT CCCAATCTCA 
GCATTATGTG CCTGTGCCTC TTCTGGATCC CATATGTCCC ATTGGGATAG CAGTCATAAG 
GAGAACAATC AAACTAAAGA CATGATTACA TACAGTGAAG TTCAGACAAG TATGCAGGAT 
GAACAACAAG CTAAAGGCAC TATTACATAC AGTGATGGTC AGGTAAAAAC TCATGTGTTT 
GCAGAACAAA AGTCCAATCA CCAAGGCACC AGGAGTATCA GACAAGAAGG TGGTCAGATG 
TCAGCGCTGA TCAAGAATAG CGAAATATCT GAAGACAATA TTACAGATGA TATAAGATCT 
CTGTTAAGGC CTCATCCTGA GTGTCCAGTT CAGTTTTCGG GCCCAGCCCT TATAAAGGAT 
GTTAGTGAGC TGGAGAGAGT GCAGTGCCAT TCAACTAAAC TGGTAAAAGA GATGGGAGAA 
TTTAGCTATG AGGAAAGACA ATCAAGTGCA AGCTTTCCCC TGCAGCTCTC ATTCAAACAG 
GGAGTTTCCA AAGACTTGGA GGACATCACT TCAACCTATG TCTATAGCCC TGGGATTCAT 
ACTGCATGCA TAGGCAATAA CAATGTTCTG AAGTCTGTGT ATACGTATAA AGGTTTGCAA 
ACTGACAGCC TTCATGCTGA TATCATGAAT GGTAATGAGT GCAAATGCAC AGAAGAGACC 
AGCAATCATC TTAATGACGG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891135.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746995.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X28, mRNA.

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CloneID	OXa64402
Clone ID Related Accession (Same CDS sequence)	XM_031891135.1
Accession Version	XM_031891135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA AATTCTCAGG ATAAAGAACA AACTAAAGAA 
ACTATTACAT ACAGGGAAAG CCAGATAAAT ACTCAGAATT TAGAAAATGT TGGCCGGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GTATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTGTC CGAAGCTACA 
ACTCTATACA AAAATGTCTC AGGAGGCTCC ATCTCAGCCT CTGCATCATC TGCATTGTCC 
TCTTCTGGAT ACCTTGCATC CTCAATGGAA AGCAGTGTTA AGGATAAAGA GCAAACCAAA 
GACACTTTTA CATACATTGA AGCTCAGATA AACTCTCAGG GCAAAGAGCA AACTAAAGAT 
ATTATTACAT ACAGTGAAGC TCAGAGACAT TTTCAGGATA AAGAGCAACC TAAAGAGAGT 
ATTACATGTA GTGAAGTTTA TAGAAGTACT CAGGATAAAG AGCAAACTAA AGACACAATT 
ACATACAGTA AAGTTCAGAG AAATATGCAG AATCTAGAAA ATGTTGGTCG GTCTGCCCCT 
AACAGGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TAGGAAACAC TGGACCAAGT 
CTGTATGGGG CTTCTTTAGC AGGATTATCC GAAGCTCCAA GCTTTCCATC ATCCATCTCT 
TCCCCTCCTG TATACCCTGT GTCTCCAACA GAAAACAGTC AACGGGTAAC CTCAGCAGCA 
ATTGATAAAA AGCAAACTAA AGACTCTATT ACCTACAGTG TTGGTCAGAT GAATACTCAG 
GATAAAAAGC AAACTCTATT AACCTACAGT GTTGGTCAGA TAAAGTCTCA GGATATAAAG 
CAAACTAAAG ACACTGTTAC ATATAGAGAA GGCCACATAA ACGCACAGGA TGAACAGCAA 
ATGAAAGACT CCATTACATA CAGCGCAGCC AAGATAAGTA CTCAGAATCT AGAAAATGTT 
GGTCGGTCTG CCCCTAACAG GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCGTCTTGGA 
TACTTTGGAG CAAGTCTGTA TGGGACCTCT TCACATCTGC CCTGGCTTGC TGGATTAATT 
GAAGCTCCAA GTCTATGTAA CACTTGCTCC AGAGTCTCCA TCCCAATCTC AGCATTATGT 
GCCTGTGCCT CTTCTGGATC CCATATGTCC CATTGGGATA GCAGTCATAA GGAGAACAAT 
CAAACTAAAG ACATGATTAC ATACAGTGAA GTTCAGACAA GTATGCAGGA TGAACAACAA 
GCTAAAGGCA CTATTACATA CAGTGATGGT CAGGTAAAAA CTCATGTGTT TGCAGAACAA 
AAGTCCAATC ACCAAGGCAC CAGGAGTATC AGACAAGAAG GTGGTCAGAT GTCAGCGCTG 
ATCAAGAATA GCGAAATATC TGAAGACAAT ATTACAGATG ATATAAGATC TCTGTTAAGG 
CCTCATCCTG AGTGTCCAGT TCAGTTTTCG GGCCCAGCCC TTATAAAGGA TGTTAGTGAG 
CTGGAGAGAG TGCAGTGCCA TTCAACTAAA CTGGTAAAAG AGATGGGAGA ATTTAGCTAT 
GAGGAAAGAC AATCAAGTGC AAGCTTTCCC CTGCAGCTCT CATTCAAACA GGGAGTTTCC 
AAAGACTTGG AGGACATCAC TTCAACCTAT GTCTATAGCC CTGGGATTCA TACTGCATGC 
ATAGGCAATA ACAATGTTCT GAAGTCTGTG TATACGTATA AAGGTTTGCA AACTGACAGC 
CTTCATGCTG ATATCATGAA TGGTAATGAG TGCAAATGCA CAGAAGAGAC CAGCAATCAT 
CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746995.1
CDS	441..9398
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891135.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891135.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGGCAAAG AAGAACCTGA TTATAATGAT 
TATACTATTA CATTCAGTGA AGGTCAGAGA CATACACAGA ATAAAAAACA GTCTGATGAT 
GCTATTACAT ACAGTAAAGT TCAGATAAAT ACTCAGGATA AAGAGCATAT TAAAGACTCT 
ATTACTTACA GTGACGTTCA GATAAATACT CAGGATAAAG TGCATACTAA AGACACTATT 
ACATACAGTG ACGTTCTGAT GAATTCTAGG AGAAATATGC AGGATAAAGA GCAAACTAAA 
GACACTATTA CATACACTGA AGCGCAGAGA AATTCTCAGG ATAAAGAACA AACTAAAGAA 
ACTATTACAT ACAGGGAAAG CCAGATAAAT ACTCAGAATT TAGAAAATGT TGGCCGGTCT 
GCCTCTAACA AGCAAAATGG GATGAGCAGT GTATCTGGGG ATCATCTTGG ATACTCTGGG 
TCAGGTCTAT ATGGCGCCTC TTTACATCAG ACCAGATCTG CAGGATTGTC CGAAGCTACA 
ACTCTATACA AAAATGTCTC AGGAGGCTCC ATCTCAGCCT CTGCATCATC TGCATTGTCC 
TCTTCTGGAT ACCTTGCATC CTCAATGGAA AGCAGTGTTA AGGATAAAGA GCAAACCAAA 
GACACTTTTA CATACATTGA AGCTCAGATA AACTCTCAGG GCAAAGAGCA AACTAAAGAT 
ATTATTACAT ACAGTGAAGC TCAGAGACAT TTTCAGGATA AAGAGCAACC TAAAGAGAGT 
ATTACATGTA GTGAAGTTTA TAGAAGTACT CAGGATAAAG AGCAAACTAA AGACACAATT 
ACATACAGTA AAGTTCAGAG AAATATGCAG AATCTAGAAA ATGTTGGTCG GTCTGCCCCT 
AACAGGCAAA ATGTGATGAG CAGTGTATCT GGGGATCATC TAGGAAACAC TGGACCAAGT 
CTGTATGGGG CTTCTTTAGC AGGATTATCC GAAGCTCCAA GCTTTCCATC ATCCATCTCT 
TCCCCTCCTG TATACCCTGT GTCTCCAACA GAAAACAGTC AACGGGTAAC CTCAGCAGCA 
ATTGATAAAA AGCAAACTAA AGACTCTATT ACCTACAGTG TTGGTCAGAT GAATACTCAG 
GATAAAAAGC AAACTCTATT AACCTACAGT GTTGGTCAGA TAAAGTCTCA GGATATAAAG 
CAAACTAAAG ACACTGTTAC ATATAGAGAA GGCCACATAA ACGCACAGGA TGAACAGCAA 
ATGAAAGACT CCATTACATA CAGCGCAGCC AAGATAAGTA CTCAGAATCT AGAAAATGTT 
GGTCGGTCTG CCCCTAACAG GCAAAATGTG ATGAGCAGTG TATCTGGGGA TCGTCTTGGA 
TACTTTGGAG CAAGTCTGTA TGGGACCTCT TCACATCTGC CCTGGCTTGC TGGATTAATT 
GAAGCTCCAA GTCTATGTAA CACTTGCTCC AGAGTCTCCA TCCCAATCTC AGCATTATGT 
GCCTGTGCCT CTTCTGGATC CCATATGTCC CATTGGGATA GCAGTCATAA GGAGAACAAT 
CAAACTAAAG ACATGATTAC ATACAGTGAA GTTCAGACAA GTATGCAGGA TGAACAACAA 
GCTAAAGGCA CTATTACATA CAGTGATGGT CAGGTAAAAA CTCATGTGTT TGCAGAACAA 
AAGTCCAATC ACCAAGGCAC CAGGAGTATC AGACAAGAAG GTGGTCAGAT GTCAGCGCTG 
ATCAAGAATA GCGAAATATC TGAAGACAAT ATTACAGATG ATATAAGATC TCTGTTAAGG 
CCTCATCCTG AGTGTCCAGT TCAGTTTTCG GGCCCAGCCC TTATAAAGGA TGTTAGTGAG 
CTGGAGAGAG TGCAGTGCCA TTCAACTAAA CTGGTAAAAG AGATGGGAGA ATTTAGCTAT 
GAGGAAAGAC AATCAAGTGC AAGCTTTCCC CTGCAGCTCT CATTCAAACA GGGAGTTTCC 
AAAGACTTGG AGGACATCAC TTCAACCTAT GTCTATAGCC CTGGGATTCA TACTGCATGC 
ATAGGCAATA ACAATGTTCT GAAGTCTGTG TATACGTATA AAGGTTTGCA AACTGACAGC 
CTTCATGCTG ATATCATGAA TGGTAATGAG TGCAAATGCA CAGAAGAGAC CAGCAATCAT 
CTTAATGACG GCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891136.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746996.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X29, mRNA.

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CloneID	OXa64403
Clone ID Related Accession (Same CDS sequence)	XM_031891136.1
Accession Version	XM_031891136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGACACTATT 
ACATACACTG AAGCGCAGAG AAATTCTCAG GATAAAGAAC AAACTAAAGA AACTATTACA 
TACAGGGAAA GCCAGATAAA TACTCAGAAT TTAGAAAATG TTGGCCGGTC TGCCTCTAAC 
AAGCAAAATG GGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTGT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CAGGAGGCTC CATCTCAGCC TCTGCATCAT CTGCATTGTC CTCTTCTGGA 
TACCTTGCAT CCTCAATGGA AAGCAGTGTT AAGGATAAAG AGCAAACCAA AGACACTTTT 
ACATACATTG AAGCTCAGAT AAACTCTCAG GGCAAAGAGC AAACTAAAGA TATTATTACA 
TACAGTGAAG CTCAGAGACA TTTTCAGGAT AAAGAGCAAC CTAAAGAGAG TATTACATGT 
AGTGAAGTTT ATAGAAGTAC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC CGAAGCTCCA AGCTTTCCAT CATCCATCTC TTCCCCTCCT 
GTATACCCTG TGTCTCCAAC AGAAAACAGT CAACGGGTAA CCTCAGCAGC AATTGATAAA 
AAGCAAACTA AAGACTCTAT TACCTACAGT GTTGGTCAGA TGAATACTCA GGATAAAAAG 
CAAACTCTAT TAACCTACAG TGTTGGTCAG ATAAAGTCTC AGGATATAAA GCAAACTAAA 
GACACTGTTA CATATAGAGA AGGCCACATA AACGCACAGG ATGAACAGCA AATGAAAGAC 
TCCATTACAT ACAGCGCAGC CAAGATAAGT ACTCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCGTCTTGG ATACTTTGGA 
GCAAGTCTGT ATGGGACCTC TTCACATCTG CCCTGGCTTG CTGGATTAAT TGAAGCTCCA 
AGTCTATGTA ACACTTGCTC CAGAGTCTCC ATCCCAATCT CAGCATTATG TGCCTGTGCC 
TCTTCTGGAT CCCATATGTC CCATTGGGAT AGCAGTCATA AGGAGAACAA TCAAACTAAA 
GACATGATTA CATACAGTGA AGTTCAGACA AGTATGCAGG ATGAACAACA AGCTAAAGGC 
ACTATTACAT ACAGTGATGG TCAGGTAAAA ACTCATGTGT TTGCAGAACA AAAGTCCAAT 
CACCAAGGCA CCAGGAGTAT CAGACAAGAA GGTGGTCAGA TGTCAGCGCT GATCAAGAAT 
AGCGAAATAT CTGAAGACAA TATTACAGAT GATATAAGAT CTCTGTTAAG GCCTCATCCT 
GAGTGTCCAG TTCAGTTTTC GGGCCCAGCC CTTATAAAGG ATGTTAGTGA GCTGGAGAGA 
GTGCAGTGCC ATTCAACTAA ACTGGTAAAA GAGATGGGAG AATTTAGCTA TGAGGAAAGA 
CAATCAAGTG CAAGCTTTCC CCTGCAGCTC TCATTCAAAC AGGGAGTTTC CAAAGACTTG 
GAGGACATCA CTTCAACCTA TGTCTATAGC CCTGGGATTC ATACTGCATG CATAGGCAAT 
AACAATGTTC TGAAGTCTGT GTATACGTAT AAAGGTTTGC AAACTGACAG CCTTCATGCT 
GATATCATGA ATGGTAATGA GTGCAAATGC ACAGAAGAGA CCAGCAATCA TCTTAATGAC 
GGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746996.1
CDS	441..9149
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALLIRELKREDSGVFTAHITVNGSATDISYILNVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISLMFHDKGKKIHLAELRNHKFSRLNKDYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHVNGQKRDISYILAVFEPIPTPVIEVTSDENFPDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNSVSVVLKSYSGRQTGIQPKIRPEIQQKIQTETQPEIKTEIRPGIKTEIQPEIKTEIQPGIKTEKQPGILTEIRPGIKTEMRPGVQKEIQLGIQKEIRSEIQTEIRQGIQRETQKETEIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891136.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891136.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTAATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAC 
CTACCAACAC GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAATTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGG 
TCTAACAGTG GCACCGCATT ACTGATCAGA GAGCTAAAAA GAGAGGATAG TGGGGTCTTC 
ACTGCTCACA TTACTGTTAA TGGAAGCGCT ACAGACATTA GTTATATACT GAATGTACTG 
GGAAGAGGAG ACATCATTCT GCCAGTGACT GGCCTTGTAG GGGGGAATGT CACACTGCCA 
ACCTATCTGA CTCTGCCAGC TGCCATCGAA AGAATCTCAT TGATGTTTCA TGACAAAGGC 
AAAAAAATAC ACCTGGCTGA ATTAAGAAAC CATAAGTTTA GCAGACTGAA CAAGGATTAT 
TTCTACAACC GCCTTGAAAA ATCTAATGAT GGAACCGCAT TACAGATCAG GGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTCATGTTA ATGGACAAAA AAGAGACATT 
TCTTATATTC TAGCCGTGTT CGAGCCTATC CCAACACCAG TAATAGAAGT GACATCTGAT 
GAGAATTTCC CTGATCTCTG CCATCTTCAT TGTTCTGTCC CATCAAACTC ACCAACCCTG 
TCCTACAGTT GGACCTACAG AGATACTGGC ACTGACAGAC TATATGCCAA TGGCAGCACA 
ATCACTGTAT CACTGAAGGA TAAGGCATTG GGGGCAGAAT TTACCTGCTG GGTACAGAAT 
CCTGCTCACA GGAACAGTGT GTCAGTCGTT TTAAAGTCAT ATAGTGGGAG ACAAACAGGA 
ATACAACCAA AGATACGACC AGAGATACAA CAAAAAATAC AAACGGAGAC ACAACCAGAG 
ATAAAAACAG AGATACGACC AGGGATAAAA ACAGAGATAC AACCAGAGAT AAAAACAGAA 
ATACAGCCAG GGATAAAAAC AGAGAAACAA CCAGGGATAC TAACGGAGAT ACGACCAGGG 
ATAAAAACAG AGATGCGACC AGGGGTGCAA AAAGAGATAC AACTAGGAAT ACAAAAAGAA 
ATACGATCAG AGATACAAAC TGAGATACGA CAAGGGATAC AAAGAGAGAC ACAAAAAGAG 
ACAGAAATAA AAGCAGGAGG AGACATCCAG GTGATTGGCC GTGTAGGGGA GACTGTCACA 
CTGCAGTGCC CTCTGAATCT ACCAACAAGA ATCTTGTGGA CGTATGATGA TAATGGGGAA 
AAAATACTCC TGGCTGAATA CAGAGACCAG AAGTTTATCA GAGTGAATAA CAACTACTTC 
ATCAACCGGC TGGAAGGATC TAACAGTGGC ACTGCATTAA GGATCAAAAA CCTGAGAATG 
GAGGATGGTG GGGTCTTCAC TGCTCACATC ACTGTTAATG GAAGCGCTAC AGAAATTAGT 
TATATTGTCA ATGTATTTGG AAGAGGAGAC ATCATTGTCC AGGCAACTGG GCGTGAAGGG 
GACACTGTCA CACTGCCAAC CTATATGAAT CACGCAACTT CCATTGAAAG AATCTCATGG 
ATGTTTCATG ACAAAGGGAA GAAAATACAC CTGGCTGAAT TCAGAAACCA CAAGTTTATC 
AGAGTAAATA AGAATTATTT CCCCAACCGT CTTGAAAAAT CGAATTATGG AACCGCATTG 
CAGATCAAAG AGCTGAGAAT GGAGGATTCT GGGATCTTCA CTGCACACAT TTATGCTAAT 
GGAAGAAAGA GAGACATTTC TTATATTCTT GTTGTGTTCG AGCTTATACC AACACCAGTA 
ATAGAAGTGA CGTATGACGA GAATTTTACT GATCTCTGCC ATCTTCATTG TTCTGTCAAT 
TCAAACTCAA CAACCCTGTC CTACAGTTGG ACCTACAGAG ACAATGGCAC TGACATACTA 
TATGCCAATG GCAGCACAAT CAGTATATTA CTAAAGAATA TGGCATCAGG CTCAGAATTT 
ACCTGCTTGG TACAAAATCC TGTTCACAGG AACAGTGCGT CAGTCCTTTT AAAGTCACTT 
GGTGGAAGAC GAACAGATAT ACGAAAAGAG ATACAAACTG AGATACGACA AGGAATACAA 
AGAGAGATAC AGCCGGAGAT ACAAACTGAG ATACGACCAG GGATACAAAG AGAGATACAG 
CCGGAGATAC AAACTGAGAT ACGAACAGGG ATACAAAGAG AGATACAAAC TGAGATACGA 
CCAGGGATAC AAAGAGAGAT ACAGCCGGAG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAAAGAG AGATACAGCC GGAGATACAA 
ACGGAGATAC AAACAGAGAT ACGACAAGGG ATACAGAGAG AGATACAGCC GGAGATACAA 
ACAGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCAA AGATACAAAC AGAGATACAA 
ACTGAGATAC GACCAGGGAT ACAGAGAGAG ATACAGCCGG AGATACAAAC TGAGATACGA 
CCAGGGATAC AGAGAGAGAT ACAGCCAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGATAC AGCCGGAGAT ACAAACAGAG ATACAAACTG AGATACGACC AGGGATACAA 
AAAGAGACAC GACGAAAGAT ACATAAAGAG ATAGAACCAA ATATACAAAC AGAGATAGAA 
AAAGAAATGC AACCAGACAT ACGACCAGAA ATACATACAG AGATAGAAAA AGATATTCAA 
CCAGAGATAC AATCAGAAAC ACCGAGAGAG GCACAAATAA AAGGACCGAA AAAAGATTCT 
CGTATACTGA CAGTAATAAT TGCAGTTTCG ATACTGTGCG GAATATTTGT TGGTATAGCT 
CTTTATTATG GAATAAGACG ACGACTAAAG GTTAAAAATC AGTCTAATTG CACTATTACA 
TACAGTGAAG TTCTGACAAG TACTCAGGAT AAAGAGCATA TTAAAGACTC TATTACATAC 
AGTGAAGTTC AGATAAATAC TCAGGATAAA GTGCATACTA AAGACACTAT TACATACAGT 
GACGTTCAGA TAAATTCTAG GAGAAATATG CAGGATAAAG AGCAAACTAA AGACACTATT 
ACATACACTG AAGCGCAGAG AAATTCTCAG GATAAAGAAC AAACTAAAGA AACTATTACA 
TACAGGGAAA GCCAGATAAA TACTCAGAAT TTAGAAAATG TTGGCCGGTC TGCCTCTAAC 
AAGCAAAATG GGATGAGCAG TGTATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTGT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CAGGAGGCTC CATCTCAGCC TCTGCATCAT CTGCATTGTC CTCTTCTGGA 
TACCTTGCAT CCTCAATGGA AAGCAGTGTT AAGGATAAAG AGCAAACCAA AGACACTTTT 
ACATACATTG AAGCTCAGAT AAACTCTCAG GGCAAAGAGC AAACTAAAGA TATTATTACA 
TACAGTGAAG CTCAGAGACA TTTTCAGGAT AAAGAGCAAC CTAAAGAGAG TATTACATGT 
AGTGAAGTTT ATAGAAGTAC TCAGGATAAA GAGCAAACTA AAGACACAAT TACATACAGT 
AAAGTTCAGA GAAATATGCA GAATCTAGAA AATGTTGGTC GGTCTGCCCC TAACAGGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTAGGAAACA CTGGACCAAG TCTGTATGGG 
GCTTCTTTAG CAGGATTATC CGAAGCTCCA AGCTTTCCAT CATCCATCTC TTCCCCTCCT 
GTATACCCTG TGTCTCCAAC AGAAAACAGT CAACGGGTAA CCTCAGCAGC AATTGATAAA 
AAGCAAACTA AAGACTCTAT TACCTACAGT GTTGGTCAGA TGAATACTCA GGATAAAAAG 
CAAACTCTAT TAACCTACAG TGTTGGTCAG ATAAAGTCTC AGGATATAAA GCAAACTAAA 
GACACTGTTA CATATAGAGA AGGCCACATA AACGCACAGG ATGAACAGCA AATGAAAGAC 
TCCATTACAT ACAGCGCAGC CAAGATAAGT ACTCAGAATC TAGAAAATGT TGGTCGGTCT 
GCCCCTAACA GGCAAAATGT GATGAGCAGT GTATCTGGGG ATCGTCTTGG ATACTTTGGA 
GCAAGTCTGT ATGGGACCTC TTCACATCTG CCCTGGCTTG CTGGATTAAT TGAAGCTCCA 
AGTCTATGTA ACACTTGCTC CAGAGTCTCC ATCCCAATCT CAGCATTATG TGCCTGTGCC 
TCTTCTGGAT CCCATATGTC CCATTGGGAT AGCAGTCATA AGGAGAACAA TCAAACTAAA 
GACATGATTA CATACAGTGA AGTTCAGACA AGTATGCAGG ATGAACAACA AGCTAAAGGC 
ACTATTACAT ACAGTGATGG TCAGGTAAAA ACTCATGTGT TTGCAGAACA AAAGTCCAAT 
CACCAAGGCA CCAGGAGTAT CAGACAAGAA GGTGGTCAGA TGTCAGCGCT GATCAAGAAT 
AGCGAAATAT CTGAAGACAA TATTACAGAT GATATAAGAT CTCTGTTAAG GCCTCATCCT 
GAGTGTCCAG TTCAGTTTTC GGGCCCAGCC CTTATAAAGG ATGTTAGTGA GCTGGAGAGA 
GTGCAGTGCC ATTCAACTAA ACTGGTAAAA GAGATGGGAG AATTTAGCTA TGAGGAAAGA 
CAATCAAGTG CAAGCTTTCC CCTGCAGCTC TCATTCAAAC AGGGAGTTTC CAAAGACTTG 
GAGGACATCA CTTCAACCTA TGTCTATAGC CCTGGGATTC ATACTGCATG CATAGGCAAT 
AACAATGTTC TGAAGTCTGT GTATACGTAT AAAGGTTTGC AAACTGACAG CCTTCATGCT 
GATATCATGA ATGGTAATGA GTGCAAATGC ACAGAAGAGA CCAGCAATCA TCTTAATGAC 
GGCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100486501 ( XM_031891137.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746997.1 Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X30, mRNA.

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CloneID	OXa64404
Clone ID Related Accession (Same CDS sequence)	XM_031891137.1
Accession Version	XM_031891137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein LOC100486501 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAGTTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGA 
TCTAACAGTG GCACTGCATT AAGGATCAAA AACCTGAGAA TGGAGGATGG TGGGGTCTTC 
ACTGCTCACA TCACTGTTAA TGGAAGCGCT ACAGAAATTA GTTATATTGT CAATGTATTT 
GGAAGAGGAG ACATCATTGT CCAGGCAACT GGGCGTGAAG GGGACACTGT CACACTGCCA 
ACCTATATGA ATCACGCAAC TTCCATTGAA AGAATCTCAT GGATGTTTCA TGACAAAGGG 
AAGAAAATAC ACCTGGCTGA ATTCAGAAAC CACAAGTTTA TCAGAGTAAA TAAGAATTAT 
TTCCCCAACC GTCTTGAAAA ATCGAATTAT GGAACCGCAT TGCAGATCAA AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTTATGCTA ATGGAAGAAA GAGAGACATT 
TCTTATATTC TTGTTGTGTT CGAGCTTATA CCAACACCAG TAATAGAAGT GACGTATGAC 
GAGAATTTTA CTGATCTCTG CCATCTTCAT TGTTCTGTCA ATTCAAACTC AACAACCCTG 
TCCTACAGTT GGACCTACAG AGACAATGGC ACTGACATAC TATATGCCAA TGGCAGCACA 
ATCAGTATAT TACTAAAGAA TATGGCATCA GGCTCAGAAT TTACCTGCTT GGTACAAAAT 
CCTGTTCACA GGAACAGTGC GTCAGTCCTT TTAAAGTCAC TTGGTGGAAG ACGAACAGAT 
ATACGAAAAG AGATACAAAC TGAGATACGA CAAGGAATAC AAAGAGAGAT ACAGCCGGAG 
ATACAAACTG AGATACGACC AGGGATACAA AGAGAGATAC AGCCGGAGAT ACAAACTGAG 
ATACGAACAG GGATACAAAG AGAGATACAA ACTGAGATAC GACCAGGGAT ACAAAGAGAG 
ATACAGCCGG AGATACAAAG AGAGATACAG CCGGAGATAC AAACGGAGAT ACAAACAGAG 
ATACGACAAG GGATACAAAG AGAGATACAG CCGGAGATAC AAACGGAGAT ACAAACAGAG 
ATACGACAAG GGATACAGAG AGAGATACAG CCGGAGATAC AAACAGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC AAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GGAGATACAA ACTGAGATAC GACCAGGGAT ACAGAGAGAG 
ATACAGCCAG AGATACAAAC TGAGATACGA CCAGGGATAC AAAAAGAGAT ACAGCCGGAG 
ATACAAACAG AGATACAAAC TGAGATACGA CCAGGGATAC AAAAAGAGAC ACGACGAAAG 
ATACATAAAG AGATAGAACC AAATATACAA ACAGAGATAG AAAAAGAAAT GCAACCAGAC 
ATACGACCAG AAATACATAC AGAGATAGAA AAAGATATTC AACCAGAGAT ACAATCAGAA 
ACACCGAGAG AGGCACAAAT AAAAGGACCG AAAAAAGATT CTCGTATACT GACAGTAATA 
ATTGCAGTTT CGATACTGTG CGGAATATTT GTTGGTATAG CTCTTTATTA TGGAATAAGA 
CGACGACTAA AGGTTAAAAA TCAGTCTAAT TGCACTATTA CATACAGTGA AGTTCTGACA 
AGTACTCAGG ATAAAGAGCA TATTAAAGAC TCTATTACAT ACAGTGAAGT TCAGATAAAT 
ACTCAGGATA AAGTGCATAC TAAAGACACT ATTACATACA GTGACGTTCA GATAAATTCT 
AGGAGAAATA TGCAGGGCAA AGAAGAACCT GATTATAATG ATTATACTAT TACATTCAGT 
GAAGGTCAGA GACATACACA GAATAAAAAA CAGTCTGATG ATGCTATTAC ATACAGTAAA 
GTTCAGATAA ATACTCAGGA TAAAGAGCAT ATTAAAGACT CTATTACTTA CAGTGACGTT 
CAGATAAATA CTCAGGATAA AGTGCATACT AAAGACACTA TTACATACAG TGACGTTCTG 
ATGAATTCTA GGAGAAATAT GCAGGATAAA GAGCAAACTA AAGGCACTAT TACATACACT 
GAAGCGCAGA GACATTCTCA GAATTTAGAA AATGTTGACC AGTCTGCATC TAACAAGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCTGGAG TCTCCATCTC AGCCTCTGCA TCATCTGCAT CATCCTCTTC TGGATACCTT 
GTATCCCCAA TGGAAAGCAG TCTTAAGAAT TTAGAAAGTG TTGGTCAGTC TGCCTCTAAC 
AAGCAAAATG GGATGAGCAG TGAATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCTCAGCC TCTGCATCAT CTGCATCGTC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAACAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCTGG AGTCTCCATC CCAGCCTCTG CATCGTCCTC TTCTGGATAC 
CTTGCATCCC TAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGAAA TTCTCAGGAT AAAGAACAAA CTAAAGAAAC TATTACATAC 
AGGGAAAGCC AGATAAATAC TCAGAATTTA GAAAATGTTG GCCGGTCTGC CTCTAACAAG 
CAAAATGGGA TGAGCAGTGT ATCTGGGGAT CATCTTGGAT ACTCTGGGTC AGGTCTATAT 
GGCGCCTCTT TACATCAGAC CAGATCTGCA GGATTGTCCG AAGCTACAAC TCTATACAAA 
AATGTCTCAG GAGGCTCCAT CTCAGCCTCT GCATCATCTG CATTGTCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA CTCTCAGGGC AAAGAGCAAA CTAAAGATAT TATTACATAC 
AGTGAAGCTC AGAGACATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCCGA AGCTCCAAGC TTTCCATCAT CCATCTCTTC CCCTCCTGTA 
TACCCTGTGT CTCCAACAGA AAACAGTCAA CGGGTAACCT CAGCAGCAAT TGATAAAAAG 
CAAACTAAAG ACTCTATTAC CTACAGTGTT GGTCAGATGA ATACTCAGGA TAAAAAGCAA 
ACTCTATTAA CCTACAGTGT TGGTCAGATA AAGTCTCAGG ATATAAAGCA AACTAAAGAC 
ACTGTTACAT ATAGAGAAGG CCACATAAAC GCACAGGATG AACAGCAAAT GAAAGACTCC 
ATTACATACA GCGCAGCCAA GATAAGTACT CAGAATCTAG AAAATGTTGG TCGGTCTGCC 
CCTAACAGGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC GTCTTGGATA CTTTGGAGCA 
AGTCTGTATG GGACCTCTTC ACATCTGCCC TGGCTTGCTG GATTAATTGA AGCTCCAAGT 
CTATGTAACA CTTGCTCCAG AGTCTCCATC CCAATCTCAG CATTATGTGC CTGTGCCTCT 
TCTGGATCCC ATATGTCCCA TTGGGATAGC AGTCATAAGG AGAACAATCA AACTAAAGAC 
ATGATTACAT ACAGTGAAGT TCAGACAAGT ATGCAGGATG AACAACAAGC TAAAGGCACT 
ATTACATACA GTGATGGTCA GGTAAAAACT CATGTGTTTG CAGAACAAAA GTCCAATCAC 
CAAGGCACCA GGAGTATCAG ACAAGAAGGT GGTCAGATGT CAGCGCTGAT CAAGAATAGC 
GAAATATCTG AAGACAATAT TACAGATGAT ATAAGATCTC TGTTAAGGCC TCATCCTGAG 
TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT ATAAAGGATG TTAGTGAGCT GGAGAGAGTG 
CAGTGCCATT CAACTAAACT GGTAAAAGAG ATGGGAGAAT TTAGCTATGA GGAAAGACAA 
TCAAGTGCAA GCTTTCCCCT GCAGCTCTCA TTCAAACAGG GAGTTTCCAA AGACTTGGAG 
GACATCACTT CAACCTATGT CTATAGCCCT GGGATTCATA CTGCATGCAT AGGCAATAAC 
AATGTTCTGA AGTCTGTGTA TACGTATAAA GGTTTGCAAA CTGACAGCCT TCATGCTGAT 
ATCATGAATG GTAATGAGTG CAAATGCACA GAAGAGACCA GCAATCATCT TAATGACGGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746997.1
CDS	442..8967
Translation	MKKFTCDILFLLWTSFLITCAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDDNGEKILLAEFRDQKFIRVNGNYFSRRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATDISYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIEKISWMFHDKGKKIHLAELRNQKFSRLNKNYFYNRLEKSNDGTALQIRELRMEDSGIFTAHIHLYGRKRDISYILAVFEPIPTPVIKVTFDENSTDLCHLHCSVPSNSPTLSYSWTYRDTGTDRLYANGSTITVSLKDKALGAEFTCWVRNPAHSNSVSVVLNSLGGRQTEIRKEIQPGMHKEIQLKIETEVRPGIQTELQPKIQTEIRPGIQTEIRTKIPTELQPEIKTKLEPGIQIDLQPKIQTELQPKIKTEIRPGIQTEIRTKIQTEIQPKIKTEIQPGIQTEIRPGIQTEIRPGIQTEIRPGIQTEIQTKLEPGIQIDLQPKIQTEIQPKIKTEIRPGIQTEIRTKIQTELRPEIKTKLEPGIQTELQPKIQTEIQPKIQTEIQPKIQTEIQSKIQTEIQPRIQTEIQPKIQTEIQPRIQTEIQPRIQTEILPELRTTIKTDTKSEIQTDIKIQTEEKIKAGGEIQVIGRLGETVTLQCPLNSPAKISWMFDNNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKHLRMEDGGVFTAHITVNGSATEIIYVLTVLGRGDIILPVTGLVGGNVTLPTYLTLPAAIERISWMFHDKGKRIHLAEFRHQKFITVNKKYFLNRLEKPNDGTALRIRELRMEDSGIFTAHIHFNGRKRDISYILAVFEPIPTPVIKVTFDENSPDLCHLHCSVPSNSPTLSYSWTYRDTGNDRLYANGSTITVSLKDKALGAEFTCWVQNPAHRNNASVPLKSLHGRRTGIQTEIQPWIQRKIQSKIQKEIRPDIKAEIQHKIQTDTKLGTQTEIQPGTEIHPEIQPEIQIELRPRIQTEIQPDIKAEIRPKIQAGIHTELQPKIKTEIRPGTQTELRPEIKTELRPEIKTELRPEIKTEIRPGTQTELRPEIKTELRPEIKTELRPGIKTEIRPGTQTEIRPEIKTELRPEIKTEIRPGTQTELRPGIQTELRPEIKTELRPEIKTELRPEIKTELRPEIKTEIQQGIQTELRPEIKTELVPGIRTDIQPGIQTDLRTEIQKKIQTDTKPEILTEVQTDIKRQTKAKIKAGGDIQVIGRVGETVTLQCPLNLPTRILWTYDDNGEKILLAEYRDQKFIRVNNNYFINRLEGSNSGTALRIKNLRMEDGGVFTAHITVNGSATEISYIVNVFGRGDIIVQATGREGDTVTLPTYMNHATSIERISWMFHDKGKKIHLAEFRNHKFIRVNKNYFPNRLEKSNYGTALQIKELRMEDSGIFTAHIYANGRKRDISYILVVFELIPTPVIEVTYDENFTDLCHLHCSVNSNSTTLSYSWTYRDNGTDILYANGSTISILLKNMASGSEFTCLVQNPVHRNSASVLLKSLGGRRTDIRKEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPEIQTEIRTGIQREIQTEIRPGIQREIQPEIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIQTEIRQGIQREIQPEIQTEIRPGIQREIQPKIQTEIQTEIRPGIQREIQPEIQTEIRPGIQREIQPEIQTEIRPGIQKEIQPEIQTEIQTEIRPGIQKETRRKIHKEIEPNIQTEIEKEMQPDIRPEIHTEIEKDIQPEIQSETPREAQIKGPKKDSRILTVIIAVSILCGIFVGIALYYGIRRRLKVKNQSNCTITYSEVLTSTQDKEHIKDSITYSEVQINTQDKVHTKDTITYSDVQINSRRNMQGKEEPDYNDYTITFSEGQRHTQNKKQSDDAITYSKVQINTQDKEHIKDSITYSDVQINTQDKVHTKDTITYSDVLMNSRRNMQDKEQTKGTITYTEAQRHSQNLENVDQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLVSPMESSLKNLESVGQSASNKQNGMSSESGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSISASASSASSSSGYLASPMESSVKNLENVGQSASNKQNVMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGVSIPASASSSSGYLASLMESSVKDKEQTKDTITYTEAQRNSQDKEQTKETITYRESQINTQNLENVGRSASNKQNGMSSVSGDHLGYSGSGLYGASLHQTRSAGLSEATTLYKNVSGGSISASASSALSSSGYLASSMESSVKDKEQTKDTFTYIEAQINSQGKEQTKDIITYSEAQRHFQDKEQPKESITCSEVYRSTQDKEQTKDTITYSKVQRNMQNLENVGRSAPNRQNVMSSVSGDHLGNTGPSLYGASLAGLSEAPSFPSSISSPPVYPVSPTENSQRVTSAAIDKKQTKDSITYSVGQMNTQDKKQTLLTYSVGQIKSQDIKQTKDTVTYREGHINAQDEQQMKDSITYSAAKISTQNLENVGRSAPNRQNVMSSVSGDRLGYFGASLYGTSSHLPWLAGLIEAPSLCNTCSRVSIPISALCACASSGSHMSHWDSSHKENNQTKDMITYSEVQTSMQDEQQAKGTITYSDGQVKTHVFAEQKSNHQGTRSIRQEGGQMSALIKNSEISEDNITDDIRSLLRPHPECPVQFSGPALIKDVSELERVQCHSTKLVKEMGEFSYEERQSSASFPLQLSFKQGVSKDLEDITSTYVYSPGIHTACIGNNNVLKSVYTYKGLQTDSLHADIMNGNECKCTEETSNHLNDGK

Target ORF information:

RefSeq Version	XM_031891137.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis uncharacterized LOC100486501 (LOC100486501), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891137.1

ORF Insert Sequence:

ATGAAGAAAT TCACTTGTGA TATCCTCTTT CTCTTATGGA CATCTTTCCT GATAACCTGC 
GCAGGAGGAG AAATCCAAGT TATTGGGCGT TTAGGGGAGA CTGTCACACT GCAGTGCCCT 
CTGAATTCAC CAGCAAAAAT CTCATGGATG TTTGATGATA ATGGGGAAAA AATACTTTTG 
GCTGAATTCA GAGACCAGAA GTTCATCAGA GTGAATGGGA ATTATTTCTC CAGGCGACTG 
GAAGGATCTA ACAGTGGCAC CGCATTAAGG ATCAAACATC TGAGAATGGA GGATGGTGGA 
GTGTTCACTG CTCACATCAC TGTTAATGGA AGCGCTACAG ACATTAGTTA TGTTCTCACA 
GTGTTGGGAA GAGGAGACAT CATTCTGCCA GTGACTGGCC TTGTAGGGGG GAATGTCACA 
TTGCCAACCT ATCTGACTCT GCCAGCTGCC ATCGAAAAAA TCTCATGGAT GTTTCATGAC 
AAAGGCAAAA AAATACACCT GGCTGAATTA AGAAACCAGA AGTTTAGCAG ACTGAACAAG 
AATTATTTCT ACAACCGCCT TGAAAAATCT AATGATGGAA CCGCATTACA GATCAGAGAG 
CTGAGAATGG AGGATTCTGG GATCTTCACT GCACACATTC ATTTATATGG ACGAAAAAGA 
GACATTTCTT ATATTCTAGC CGTGTTCGAG CCTATCCCAA CACCAGTGAT AAAAGTGACG 
TTTGATGAGA ATTCCACTGA TCTCTGCCAT CTTCATTGTT CTGTCCCATC AAACTCACCA 
ACCCTGTCCT ACAGTTGGAC CTACAGAGAT ACTGGCACTG ACAGACTATA TGCCAATGGC 
AGCACAATCA CTGTATCACT GAAGGATAAG GCATTGGGGG CAGAATTTAC CTGCTGGGTA 
CGAAATCCTG CTCACAGCAA TAGTGTGTCA GTCGTTTTAA ATTCACTTGG TGGGAGACAA 
ACAGAGATAC GAAAAGAGAT ACAACCAGGG ATGCATAAAG AGATACAATT AAAGATAGAA 
ACAGAGGTAC GACCAGGGAT ACAAACAGAG CTACAGCCAA AGATACAAAC AGAGATACGA 
CCAGGGATAC AAACAGAGAT ACGAACAAAG ATACCAACAG AGCTACAACC AGAGATAAAA 
ACTAAGCTAG AACCAGGGAT ACAAATAGAT CTACAACCAA AGATACAAAC AGAGCTACAA 
CCAAAGATAA AAACAGAGAT ACGACCAGGG ATACAAACAG AGATACGAAC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACAACCAG GAATACAAAC AGAGATACGA 
CCAGGAATAC AAACAGAGAT ACGGCCAGGA ATACAAACAG AGATACGGCC AGGAATACAA 
ACAGAGATAC AAACTAAGCT AGAACCAGGG ATACAAATAG ATCTACAACC AAAGATACAA 
ACAGAGATAC AACCAAAGAT AAAAACAGAG ATACGACCAG GAATACAAAC AGAGATACGA 
ACAAAGATAC AAACAGAGCT ACGACCAGAG ATAAAAACTA AGCTAGAACC AGGGATACAA 
ACAGAGCTAC AACCAAAGAT ACAAACAGAG ATACAACCAA AGATACAAAC GGAGATACAA 
CCAAAGATAC AAACAGAGAT ACAATCAAAG ATACAAACGG AGATACAACC AAGGATACAA 
ACAGAGATAC AACCAAAGAT ACAAACAGAG ATACAACCAA GGATACAAAC AGAGATACAA 
CCAAGGATAC AAACAGAGAT ACTACCGGAG TTACGAACAA CGATAAAAAC AGACACAAAA 
TCAGAAATAC AAACAGACAT AAAGATACAA ACTGAGGAAA AAATAAAAGC AGGAGGAGAA 
ATCCAAGTTA TTGGGCGTTT AGGGGAGACT GTCACACTGC AGTGCCCTCT GAATTCACCA 
GCAAAAATCT CATGGATGTT TGATAATAAT GGGGAAAAAA TTCTCCTGGC TGAATACAGA 
GACCAGAAGT TCATCAGAGT GAATAACAAC TACTTCATCA ACCGGCTGGA AGGGTCTAAC 
AGTGGCACCG CATTAAGGAT CAAACATCTG AGAATGGAGG ATGGTGGGGT CTTCACTGCT 
CACATCACTG TTAATGGAAG CGCTACAGAA ATTATTTATG TTCTCACAGT GTTGGGAAGA 
GGAGACATCA TTCTGCCAGT GACTGGCCTT GTAGGGGGGA ATGTCACACT GCCAACCTAT 
CTGACTCTGC CAGCTGCCAT CGAAAGAATC TCATGGATGT TTCATGACAA GGGGAAGCGA 
ATACACCTGG CTGAATTCAG ACACCAGAAG TTTATCACAG TTAATAAGAA ATATTTCTTA 
AACCGCCTTG AAAAACCTAA TGATGGAACC GCATTAAGGA TCAGAGAGCT GAGAATGGAG 
GATTCTGGGA TCTTCACTGC ACACATTCAT TTTAATGGAC GAAAAAGAGA CATTTCTTAC 
ATTCTAGCCG TTTTCGAGCC TATCCCAACA CCAGTGATAA AAGTGACGTT TGATGAGAAT 
TCCCCTGATC TCTGCCATCT TCATTGTTCT GTCCCATCAA ACTCACCAAC CCTGTCCTAC 
AGTTGGACCT ACAGAGATAC TGGCAATGAC AGACTATATG CCAATGGCAG CACAATCACT 
GTATCACTGA AGGATAAGGC ATTGGGGGCA GAATTTACCT GCTGGGTACA GAATCCTGCT 
CACAGGAACA ATGCATCAGT CCCTTTAAAG TCACTTCATG GGAGACGAAC AGGAATACAA 
ACTGAGATAC AACCATGGAT ACAAAGAAAG ATACAATCAA AGATACAAAA AGAGATACGA 
CCAGATATAA AAGCAGAGAT TCAACATAAA ATACAAACAG ACACAAAACT AGGAACGCAG 
ACAGAGATAC AACCTGGAAC AGAGATACAT CCAGAAATAC AACCAGAGAT ACAAATAGAG 
CTACGACCCA GGATACAAAC AGAGATACAA CCAGATATAA AAGCAGAGAT ACGACCAAAG 
ATACAAGCAG GGATACACAC TGAGCTACAA CCAAAGATAA AAACAGAGAT ACGACCAGGG 
ACACAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGAGAT AAAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGATACGA CCAGGGACAC AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGCTAC GACCAGGGAT AAAAACAGAG 
ATACGACCAG GGACACAAAC AGAGATACGA CCAGAGATAA AAACAGAGCT ACGACCAGAG 
ATAAAAACAG AGATACGACC AGGGACACAA ACAGAGCTGC GACCAGGGAT ACAAACAGAG 
CTACGACCAG AGATAAAAAC AGAGCTACGA CCAGAGATAA AAACAGAGCT TCGACCAGAG 
ATAAAAACAG AGCTACGACC AGAGATAAAA ACAGAGATAC AACAAGGGAT ACAAACAGAG 
CTACGTCCAG AGATAAAAAC TGAGCTAGTA CCAGGGATAC GTACAGATAT ACAACCAGGG 
ATACAAACAG ATCTACGAAC AGAGATACAA AAAAAGATAC AAACAGACAC CAAACCAGAA 
ATACTAACAG AGGTGCAAAC AGACATAAAG AGACAAACAA AGGCAAAAAT AAAAGCAGGA 
GGAGACATCC AGGTGATTGG CCGTGTAGGG GAGACTGTCA CACTGCAGTG CCCTCTGAAT 
CTACCAACAA GAATCTTGTG GACGTATGAT GATAATGGGG AAAAAATACT CCTGGCTGAA 
TACAGAGACC AGAAGTTTAT CAGAGTGAAT AACAACTACT TCATCAACCG GCTGGAAGGA 
TCTAACAGTG GCACTGCATT AAGGATCAAA AACCTGAGAA TGGAGGATGG TGGGGTCTTC 
ACTGCTCACA TCACTGTTAA TGGAAGCGCT ACAGAAATTA GTTATATTGT CAATGTATTT 
GGAAGAGGAG ACATCATTGT CCAGGCAACT GGGCGTGAAG GGGACACTGT CACACTGCCA 
ACCTATATGA ATCACGCAAC TTCCATTGAA AGAATCTCAT GGATGTTTCA TGACAAAGGG 
AAGAAAATAC ACCTGGCTGA ATTCAGAAAC CACAAGTTTA TCAGAGTAAA TAAGAATTAT 
TTCCCCAACC GTCTTGAAAA ATCGAATTAT GGAACCGCAT TGCAGATCAA AGAGCTGAGA 
ATGGAGGATT CTGGGATCTT CACTGCACAC ATTTATGCTA ATGGAAGAAA GAGAGACATT 
TCTTATATTC TTGTTGTGTT CGAGCTTATA CCAACACCAG TAATAGAAGT GACGTATGAC 
GAGAATTTTA CTGATCTCTG CCATCTTCAT TGTTCTGTCA ATTCAAACTC AACAACCCTG 
TCCTACAGTT GGACCTACAG AGACAATGGC ACTGACATAC TATATGCCAA TGGCAGCACA 
ATCAGTATAT TACTAAAGAA TATGGCATCA GGCTCAGAAT TTACCTGCTT GGTACAAAAT 
CCTGTTCACA GGAACAGTGC GTCAGTCCTT TTAAAGTCAC TTGGTGGAAG ACGAACAGAT 
ATACGAAAAG AGATACAAAC TGAGATACGA CAAGGAATAC AAAGAGAGAT ACAGCCGGAG 
ATACAAACTG AGATACGACC AGGGATACAA AGAGAGATAC AGCCGGAGAT ACAAACTGAG 
ATACGAACAG GGATACAAAG AGAGATACAA ACTGAGATAC GACCAGGGAT ACAAAGAGAG 
ATACAGCCGG AGATACAAAG AGAGATACAG CCGGAGATAC AAACGGAGAT ACAAACAGAG 
ATACGACAAG GGATACAAAG AGAGATACAG CCGGAGATAC AAACGGAGAT ACAAACAGAG 
ATACGACAAG GGATACAGAG AGAGATACAG CCGGAGATAC AAACAGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC AAAGATACAA ACAGAGATAC AAACTGAGAT ACGACCAGGG 
ATACAGAGAG AGATACAGCC GGAGATACAA ACTGAGATAC GACCAGGGAT ACAGAGAGAG 
ATACAGCCAG AGATACAAAC TGAGATACGA CCAGGGATAC AAAAAGAGAT ACAGCCGGAG 
ATACAAACAG AGATACAAAC TGAGATACGA CCAGGGATAC AAAAAGAGAC ACGACGAAAG 
ATACATAAAG AGATAGAACC AAATATACAA ACAGAGATAG AAAAAGAAAT GCAACCAGAC 
ATACGACCAG AAATACATAC AGAGATAGAA AAAGATATTC AACCAGAGAT ACAATCAGAA 
ACACCGAGAG AGGCACAAAT AAAAGGACCG AAAAAAGATT CTCGTATACT GACAGTAATA 
ATTGCAGTTT CGATACTGTG CGGAATATTT GTTGGTATAG CTCTTTATTA TGGAATAAGA 
CGACGACTAA AGGTTAAAAA TCAGTCTAAT TGCACTATTA CATACAGTGA AGTTCTGACA 
AGTACTCAGG ATAAAGAGCA TATTAAAGAC TCTATTACAT ACAGTGAAGT TCAGATAAAT 
ACTCAGGATA AAGTGCATAC TAAAGACACT ATTACATACA GTGACGTTCA GATAAATTCT 
AGGAGAAATA TGCAGGGCAA AGAAGAACCT GATTATAATG ATTATACTAT TACATTCAGT 
GAAGGTCAGA GACATACACA GAATAAAAAA CAGTCTGATG ATGCTATTAC ATACAGTAAA 
GTTCAGATAA ATACTCAGGA TAAAGAGCAT ATTAAAGACT CTATTACTTA CAGTGACGTT 
CAGATAAATA CTCAGGATAA AGTGCATACT AAAGACACTA TTACATACAG TGACGTTCTG 
ATGAATTCTA GGAGAAATAT GCAGGATAAA GAGCAAACTA AAGGCACTAT TACATACACT 
GAAGCGCAGA GACATTCTCA GAATTTAGAA AATGTTGACC AGTCTGCATC TAACAAGCAA 
AATGTGATGA GCAGTGTATC TGGGGATCAT CTTGGATACT CTGGGTCAGG TCTATATGGC 
GCCTCTTTAC ATCAGACCAG ATCTGCAGGA TTATCCGAAG CTACAACTCT ATACAAAAAT 
GTCTCTGGAG TCTCCATCTC AGCCTCTGCA TCATCTGCAT CATCCTCTTC TGGATACCTT 
GTATCCCCAA TGGAAAGCAG TCTTAAGAAT TTAGAAAGTG TTGGTCAGTC TGCCTCTAAC 
AAGCAAAATG GGATGAGCAG TGAATCTGGG GATCATCTTG GATACTCTGG GTCAGGTCTA 
TATGGCGCCT CTTTACATCA GACCAGATCT GCAGGATTAT CCGAAGCTAC AACTCTATAC 
AAAAATGTCT CTGGAGTCTC CATCTCAGCC TCTGCATCAT CTGCATCGTC CTCTTCTGGA 
TACCTTGCAT CCCCAATGGA AAGCAGTGTT AAGAATTTAG AAAATGTTGG TCAGTCTGCT 
TCTAACAAGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC ATCTTGGATA CTCTGGGTCA 
GGTCTATATG GCGCCTCTTT ACATCAGACC AGATCTGCAG GATTATCCGA AGCTACAACT 
CTATACAAAA ATGTCTCTGG AGTCTCCATC CCAGCCTCTG CATCGTCCTC TTCTGGATAC 
CTTGCATCCC TAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACTAAAGA CACTATTACA 
TACACTGAAG CGCAGAGAAA TTCTCAGGAT AAAGAACAAA CTAAAGAAAC TATTACATAC 
AGGGAAAGCC AGATAAATAC TCAGAATTTA GAAAATGTTG GCCGGTCTGC CTCTAACAAG 
CAAAATGGGA TGAGCAGTGT ATCTGGGGAT CATCTTGGAT ACTCTGGGTC AGGTCTATAT 
GGCGCCTCTT TACATCAGAC CAGATCTGCA GGATTGTCCG AAGCTACAAC TCTATACAAA 
AATGTCTCAG GAGGCTCCAT CTCAGCCTCT GCATCATCTG CATTGTCCTC TTCTGGATAC 
CTTGCATCCT CAATGGAAAG CAGTGTTAAG GATAAAGAGC AAACCAAAGA CACTTTTACA 
TACATTGAAG CTCAGATAAA CTCTCAGGGC AAAGAGCAAA CTAAAGATAT TATTACATAC 
AGTGAAGCTC AGAGACATTT TCAGGATAAA GAGCAACCTA AAGAGAGTAT TACATGTAGT 
GAAGTTTATA GAAGTACTCA GGATAAAGAG CAAACTAAAG ACACAATTAC ATACAGTAAA 
GTTCAGAGAA ATATGCAGAA TCTAGAAAAT GTTGGTCGGT CTGCCCCTAA CAGGCAAAAT 
GTGATGAGCA GTGTATCTGG GGATCATCTA GGAAACACTG GACCAAGTCT GTATGGGGCT 
TCTTTAGCAG GATTATCCGA AGCTCCAAGC TTTCCATCAT CCATCTCTTC CCCTCCTGTA 
TACCCTGTGT CTCCAACAGA AAACAGTCAA CGGGTAACCT CAGCAGCAAT TGATAAAAAG 
CAAACTAAAG ACTCTATTAC CTACAGTGTT GGTCAGATGA ATACTCAGGA TAAAAAGCAA 
ACTCTATTAA CCTACAGTGT TGGTCAGATA AAGTCTCAGG ATATAAAGCA AACTAAAGAC 
ACTGTTACAT ATAGAGAAGG CCACATAAAC GCACAGGATG AACAGCAAAT GAAAGACTCC 
ATTACATACA GCGCAGCCAA GATAAGTACT CAGAATCTAG AAAATGTTGG TCGGTCTGCC 
CCTAACAGGC AAAATGTGAT GAGCAGTGTA TCTGGGGATC GTCTTGGATA CTTTGGAGCA 
AGTCTGTATG GGACCTCTTC ACATCTGCCC TGGCTTGCTG GATTAATTGA AGCTCCAAGT 
CTATGTAACA CTTGCTCCAG AGTCTCCATC CCAATCTCAG CATTATGTGC CTGTGCCTCT 
TCTGGATCCC ATATGTCCCA TTGGGATAGC AGTCATAAGG AGAACAATCA AACTAAAGAC 
ATGATTACAT ACAGTGAAGT TCAGACAAGT ATGCAGGATG AACAACAAGC TAAAGGCACT 
ATTACATACA GTGATGGTCA GGTAAAAACT CATGTGTTTG CAGAACAAAA GTCCAATCAC 
CAAGGCACCA GGAGTATCAG ACAAGAAGGT GGTCAGATGT CAGCGCTGAT CAAGAATAGC 
GAAATATCTG AAGACAATAT TACAGATGAT ATAAGATCTC TGTTAAGGCC TCATCCTGAG 
TGTCCAGTTC AGTTTTCGGG CCCAGCCCTT ATAAAGGATG TTAGTGAGCT GGAGAGAGTG 
CAGTGCCATT CAACTAAACT GGTAAAAGAG ATGGGAGAAT TTAGCTATGA GGAAAGACAA 
TCAAGTGCAA GCTTTCCCCT GCAGCTCTCA TTCAAACAGG GAGTTTCCAA AGACTTGGAG 
GACATCACTT CAACCTATGT CTATAGCCCT GGGATTCATA CTGCATGCAT AGGCAATAAC 
AATGTTCTGA AGTCTGTGTA TACGTATAAA GGTTTGCAAA CTGACAGCCT TCATGCTGAT 
ATCATGAATG GTAATGAGTG CAAATGCACA GAAGAGACCA GCAATCATCT TAATGACGGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100486501 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: