trim66 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim66
Entrez Gene ID	100485708
Full Name	tripartite motif containing 66
General protein information	Preferred Names tripartite motif containing 66 Names tripartite motif-containing protein 66
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004913328.4	XP_004913385.2	tripartite motif-containing protein 66 isoform X2
XM_004913328.3	XP_004913385.1	tripartite motif-containing protein 66 isoform X2
XM_004913327.4	XP_004913384.2	tripartite motif-containing protein 66 isoform X1
XM_004913327.3	XP_004913384.1	tripartite motif-containing protein 66 isoform X1
XM_002942863.4	XP_002942909.2	tripartite motif-containing protein 66 isoform X3
XM_002942863.5	XP_002942909.3	tripartite motif-containing protein 66 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRIM66	XP_005633693.1
Bos taurus (cattle)	TRIM66	XP_002693153.3
Mus musculus (house mouse)	Trim66	NP_001164383.1
Gallus gallus (chicken)	TRIM66	XP_004941449.1
Xenopus tropicalis (tropical clawed frog)	trim66	XP_002942909.2
Homo sapiens (human)	TRIM66	NP_055633.1
Rattus norvegicus (Norway rat)	Trim66	NP_001178768.1
	TRIM66	XP_001097566.2
Pan troglodytes (chimpanzee)	TRIM66	XP_003313014.2

Gene Ontology

The following trim66 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim66 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa08457	XM_004913327.3 Latest version!	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa08458	XM_004913328.3 Latest version!	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa08459	XM_002942863.4 Latest version!	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56366	XM_004913327.4 Latest version!	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56367	XM_004913328.4 Latest version!	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56368	XM_002942863.5 Latest version!	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim66 ( XM_004913327.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913384.1 Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X1, mRNA.

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CloneID	OXa08457
Clone ID Related Accession (Same CDS sequence)	XM_004913327.3
Accession Version	XM_004913327.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913327.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAATG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTAACCGTT GCCCAAAAGT CTTTCACCTT TCCTGGCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATA TAGGGCCCGC AGAACAGAAG GCAATATTCA AGGAAGACTC TGAAGTGGCC 
GAAGCCGGAC GGAACCTGGA GATGTACTTT GAGAACATGC TGAAGGAGGC CTACCCCGAG 
AAGGTCTTCC CTTTCCCACA GGAGGAAGAT TCCGACACGG AGGAGATCGG CAGTGAGAGC 
TGCCACCTGA GCCTGAAAGG CTTTCACTGG CCACCCTACG GGCCGGACTA CCTGCAGCCC 
AAGCGTAGGA GGCGCCATAC CCTTAGCCAG AAGCCCAGGG AGTTCGACAT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913384.1
CDS	861..4517
Translation	MAKSCFDCTEKNSAHSLCITCNKWLCSTCAEQHRHTKSVSDPFIPAVQRGSAGNDGTSGNFLCCPLHHQESLKLFCETCDTLVCRHCVVMEHKDHRFRRLDEALQHQRAVLENVITQVEEKKVVVQAAGKQIEDRLYEIKHMHSKVENQIKVTKMVLINELNKRTNVLLEQLEKITTEKRHKYEQQLQSILVLNRQLEHVQNFINWAMYGKNSIPFLFSKELIVYQMQRLLDTSCGGDVGRTSKIRFSWEPSFWTKQISNLGCLMADGAHLPTPDIPTYGTENAQAPYYPAPHVPITAHPGTITSSPHYTSSVQCPTPMCCTHCHTLPTVPKNQPATGTVTPHVSFAHPTPIKQQQPPSTQYNSSKEQKPTVRPLRVIQPWVSHQPQAEQENSPYWLDHQQQKPQVPPSQPNAPPVCPVPPQDFNQVHNVHTIQHTVPITTPTSLQAPSVLQTPSVLQAPSVQMQVSSVHSLSHIQPQRLQQQSALCQAESAHEQVMHHSLDIINQQFELEQMQKGLELLLQSQPSNVQLNPTKQPQHVQQTIVGQINYIVRQPATAPLQPPEEIPQVCDEPISPGSHTVDLSPVTTSSPSNMQSQSIADEIIAAVENDACQRNQLAANPERKRSASVGFCNVPEMELSSPRLSRSVDPQMPTVSCQFFEPPEDRGFLPGTEAERLAEYTAVEAAVADNIHVATEDHQATGAAVLGNAICKVENEDFSCANSAAIDTDLQTESAPPVISSEFILPLPEAFEEPINLSVKKCPPPETSSVIVKKPTHLPPAPGKPLKHETEDRGFKPSFAEGRKGKENTPRTPARETRIPYVRLERLKIQAPNSGELPVFKVQPQKKDEDGSVRLVVKYGAQSKSMSIKVNPDCPYSSPHPAPQPAMPYGTEPIPYTTSQTISELEQQVNNLARVSPYSGESPAFRNRIEAPAAPIEDPEVTCNVNAAPQPTRKPVQSEDHDQLENEDFCAVCLNGGEMLCCNRCPKVFHLSWHVPALLSFPVGEWLCTLCRNLTKPEVEYDCDNVRYCLENKMEIAAFPNLDDYDQRKCETLVLSLCCNSLSLPFQEPVSPLARHYYQIIKRPMDLSIIRKKLQRRNIPHYSAPEELVYDIRLMFWNCAKFNYIGPAEQKAIFKEDSEVAEAGRNLEMYFENMLKEAYPEKVFPFPQEEDSDTEEIGSESCHLSLKGFHWPPYGPDYLQPKRRRRHTLSQKPREFDIC

Target ORF information:

RefSeq Version	XM_004913327.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913327.3

ORF Insert Sequence:

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAATG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTAACCGTT GCCCAAAAGT CTTTCACCTT TCCTGGCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATA TAGGGCCCGC AGAACAGAAG GCAATATTCA AGGAAGACTC TGAAGTGGCC 
GAAGCCGGAC GGAACCTGGA GATGTACTTT GAGAACATGC TGAAGGAGGC CTACCCCGAG 
AAGGTCTTCC CTTTCCCACA GGAGGAAGAT TCCGACACGG AGGAGATCGG CAGTGAGAGC 
TGCCACCTGA GCCTGAAAGG CTTTCACTGG CCACCCTACG GGCCGGACTA CCTGCAGCCC 
AAGCGTAGGA GGCGCCATAC CCTTAGCCAG AAGCCCAGGG AGTTCGACAT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim66 ( XM_004913328.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913385.1 Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa08458
Clone ID Related Accession (Same CDS sequence)	XM_004913328.3
Accession Version	XM_004913328.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tripartite motif-containing protein 66 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAC AGAGGGTTCA AGCCGAGCTT TGCAGAAGGC 
AGAAAGGGCA AGGAAAACAC ACCCAGGACG CCGGCGAGGG AAACCCGAAT CCCCTACGTG 
CGACTGGAGC GGCTAAAGAT CCAGGCTCCC AACTCCGGGG AACTCCCTGT TTTTAAGGTT 
CAGCCGCAGA AAAAAGATGA AGACGGGTCG GTGCGTTTAG TTGTCAAATA TGGCGCCCAG 
TCCAAGAGCA TGTCCATAAA GGTTAACCCA GACTGCCCGT ACTCATCCCC CCACCCCGCC 
CCGCAACCCG CAATGCCTTA TGGGACAGAA CCAATCCCTT ACACCACCAG CCAAACCATT 
TCTGAACTGG AGCAACAAGT GAACAATCTG GCGAGGGTGA GTCCCTACAG CGGGGAGAGC 
CCGGCATTCA GGAACAGAAT CGAGGCCCCG GCGGCTCCCA TAGAAGATCC TGAGGTGACG 
TGCAATGTCA ACGCTGCCCC TCAGCCAACC AGAAAGCCCG TCCAATCAGA AGACCATGAT 
CAGCTTGAGA ATGAGGATTT CTGTGCTGTT TGTCTGAATG GAGGGGAAAT GCTTTGCTGT 
AACCGTTGCC CAAAAGTCTT TCACCTTTCC TGGCATGTTC CAGCCCTGCT CAGCTTCCCT 
GTGGGCGAAT GGCTTTGCAC CTTGTGCCGA AACCTGACCA AACCAGAGGT AGAATACGAC 
TGCGACAATG TCCGCTATTG TCTAGAGAAT AAAATGGAGA TTGCGGCTTT TCCCAACCTT 
GACGACTACG ACCAAAGGAA GTGTGAGACG CTGGTGCTGT CGCTGTGCTG TAACAGTCTG 
AGCCTCCCAT TCCAGGAACC CGTCAGCCCC TTGGCGCGGC ACTACTACCA GATCATAAAG 
CGCCCGATGG ACCTCTCCAT TATACGGAAG AAGCTCCAGA GACGCAATAT CCCGCATTAC 
TCGGCGCCGG AGGAACTGGT GTACGATATT CGCCTCATGT TCTGGAACTG CGCAAAGTTT 
AATTATATAG GGCCCGCAGA ACAGAAGGCA ATATTCAAGG AAGACTCTGA AGTGGCCGAA 
GCCGGACGGA ACCTGGAGAT GTACTTTGAG AACATGCTGA AGGAGGCCTA CCCCGAGAAG 
GTCTTCCCTT TCCCACAGGA GGAAGATTCC GACACGGAGG AGATCGGCAG TGAGAGCTGC 
CACCTGAGCC TGAAAGGCTT TCACTGGCCA CCCTACGGGC CGGACTACCT GCAGCCCAAG 
CGTAGGAGGC GCCATACCCT TAGCCAGAAG CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913385.1
CDS	861..4514
Translation	MAKSCFDCTEKNSAHSLCITCNKWLCSTCAEQHRHTKSVSDPFIPAVQRGSAGNDGTSGNFLCCPLHHQESLKLFCETCDTLVCRHCVVMEHKDHRFRRLDEALQHQRAVLENVITQVEEKKVVVQAAGKQIEDRLYEIKHMHSKVENQIKVTKMVLINELNKRTNVLLEQLEKITTEKRHKYEQQLQSILVLNRQLEHVQNFINWAMYGKNSIPFLFSKELIVYQMQRLLDTSCGGDVGRTSKIRFSWEPSFWTKQISNLGCLMADGAHLPTPDIPTYGTENAQAPYYPAPHVPITAHPGTITSSPHYTSSVQCPTPMCCTHCHTLPTVPKNQPATGTVTPHVSFAHPTPIKQQQPPSTQYNSSKEQKPTVRPLRVIQPWVSHQPQAEQENSPYWLDHQQQKPQVPPSQPNAPPVCPVPPQDFNQVHNVHTIQHTVPITTPTSLQAPSVLQTPSVLQAPSVQMQVSSVHSLSHIQPQRLQQQSALCQAESAHEQVMHHSLDIINQQFELEQMQKGLELLLQSQPSNVQLNPTKQPQHVQQTIVGQINYIVRQPATAPLQPPEEIPQVCDEPISPGSHTVDLSPVTTSSPSNMQSQSIADEIIAAVENDACQRNQLAANPERKRSASVGFCNVPEMELSSPRLSRSVDPQMPTVSCQFFEPPEDRGFLPGTEAERLAEYTAVEAAVADNIHVATEDHQATGAAVLGNAICKVENEDFSCANSAAIDTDLQTESAPPVISSEFILPLPEAFEEPINLSVKKCPPPETSSVIVKKPTHLPPAPGKPLKHETDRGFKPSFAEGRKGKENTPRTPARETRIPYVRLERLKIQAPNSGELPVFKVQPQKKDEDGSVRLVVKYGAQSKSMSIKVNPDCPYSSPHPAPQPAMPYGTEPIPYTTSQTISELEQQVNNLARVSPYSGESPAFRNRIEAPAAPIEDPEVTCNVNAAPQPTRKPVQSEDHDQLENEDFCAVCLNGGEMLCCNRCPKVFHLSWHVPALLSFPVGEWLCTLCRNLTKPEVEYDCDNVRYCLENKMEIAAFPNLDDYDQRKCETLVLSLCCNSLSLPFQEPVSPLARHYYQIIKRPMDLSIIRKKLQRRNIPHYSAPEELVYDIRLMFWNCAKFNYIGPAEQKAIFKEDSEVAEAGRNLEMYFENMLKEAYPEKVFPFPQEEDSDTEEIGSESCHLSLKGFHWPPYGPDYLQPKRRRRHTLSQKPREFDIC

Target ORF information:

RefSeq Version	XM_004913328.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913328.3

ORF Insert Sequence:

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAC AGAGGGTTCA AGCCGAGCTT TGCAGAAGGC 
AGAAAGGGCA AGGAAAACAC ACCCAGGACG CCGGCGAGGG AAACCCGAAT CCCCTACGTG 
CGACTGGAGC GGCTAAAGAT CCAGGCTCCC AACTCCGGGG AACTCCCTGT TTTTAAGGTT 
CAGCCGCAGA AAAAAGATGA AGACGGGTCG GTGCGTTTAG TTGTCAAATA TGGCGCCCAG 
TCCAAGAGCA TGTCCATAAA GGTTAACCCA GACTGCCCGT ACTCATCCCC CCACCCCGCC 
CCGCAACCCG CAATGCCTTA TGGGACAGAA CCAATCCCTT ACACCACCAG CCAAACCATT 
TCTGAACTGG AGCAACAAGT GAACAATCTG GCGAGGGTGA GTCCCTACAG CGGGGAGAGC 
CCGGCATTCA GGAACAGAAT CGAGGCCCCG GCGGCTCCCA TAGAAGATCC TGAGGTGACG 
TGCAATGTCA ACGCTGCCCC TCAGCCAACC AGAAAGCCCG TCCAATCAGA AGACCATGAT 
CAGCTTGAGA ATGAGGATTT CTGTGCTGTT TGTCTGAATG GAGGGGAAAT GCTTTGCTGT 
AACCGTTGCC CAAAAGTCTT TCACCTTTCC TGGCATGTTC CAGCCCTGCT CAGCTTCCCT 
GTGGGCGAAT GGCTTTGCAC CTTGTGCCGA AACCTGACCA AACCAGAGGT AGAATACGAC 
TGCGACAATG TCCGCTATTG TCTAGAGAAT AAAATGGAGA TTGCGGCTTT TCCCAACCTT 
GACGACTACG ACCAAAGGAA GTGTGAGACG CTGGTGCTGT CGCTGTGCTG TAACAGTCTG 
AGCCTCCCAT TCCAGGAACC CGTCAGCCCC TTGGCGCGGC ACTACTACCA GATCATAAAG 
CGCCCGATGG ACCTCTCCAT TATACGGAAG AAGCTCCAGA GACGCAATAT CCCGCATTAC 
TCGGCGCCGG AGGAACTGGT GTACGATATT CGCCTCATGT TCTGGAACTG CGCAAAGTTT 
AATTATATAG GGCCCGCAGA ACAGAAGGCA ATATTCAAGG AAGACTCTGA AGTGGCCGAA 
GCCGGACGGA ACCTGGAGAT GTACTTTGAG AACATGCTGA AGGAGGCCTA CCCCGAGAAG 
GTCTTCCCTT TCCCACAGGA GGAAGATTCC GACACGGAGG AGATCGGCAG TGAGAGCTGC 
CACCTGAGCC TGAAAGGCTT TCACTGGCCA CCCTACGGGC CGGACTACCT GCAGCCCAAG 
CGTAGGAGGC GCCATACCCT TAGCCAGAAG CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim66 ( XM_002942863.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942909.2 Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X3, mRNA.

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CloneID	OXa08459
Clone ID Related Accession (Same CDS sequence)	XM_002942863.4
Accession Version	XM_002942863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tripartite motif-containing protein 66 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAATG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTAACCGTT GCCCAAAAGT CTTTCACCTT TCCTGGCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATG AAGACTCTGA AGTGGCCGAA GCCGGACGGA ACCTGGAGAT GTACTTTGAG 
AACATGCTGA AGGAGGCCTA CCCCGAGAAG GTCTTCCCTT TCCCACAGGA GGAAGATTCC 
GACACGGAGG AGATCGGCAG TGAGAGCTGC CACCTGAGCC TGAAAGGCTT TCACTGGCCA 
CCCTACGGGC CGGACTACCT GCAGCCCAAG CGTAGGAGGC GCCATACCCT TAGCCAGAAG 
CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942909.2
CDS	861..4484
Translation	MAKSCFDCTEKNSAHSLCITCNKWLCSTCAEQHRHTKSVSDPFIPAVQRGSAGNDGTSGNFLCCPLHHQESLKLFCETCDTLVCRHCVVMEHKDHRFRRLDEALQHQRAVLENVITQVEEKKVVVQAAGKQIEDRLYEIKHMHSKVENQIKVTKMVLINELNKRTNVLLEQLEKITTEKRHKYEQQLQSILVLNRQLEHVQNFINWAMYGKNSIPFLFSKELIVYQMQRLLDTSCGGDVGRTSKIRFSWEPSFWTKQISNLGCLMADGAHLPTPDIPTYGTENAQAPYYPAPHVPITAHPGTITSSPHYTSSVQCPTPMCCTHCHTLPTVPKNQPATGTVTPHVSFAHPTPIKQQQPPSTQYNSSKEQKPTVRPLRVIQPWVSHQPQAEQENSPYWLDHQQQKPQVPPSQPNAPPVCPVPPQDFNQVHNVHTIQHTVPITTPTSLQAPSVLQTPSVLQAPSVQMQVSSVHSLSHIQPQRLQQQSALCQAESAHEQVMHHSLDIINQQFELEQMQKGLELLLQSQPSNVQLNPTKQPQHVQQTIVGQINYIVRQPATAPLQPPEEIPQVCDEPISPGSHTVDLSPVTTSSPSNMQSQSIADEIIAAVENDACQRNQLAANPERKRSASVGFCNVPEMELSSPRLSRSVDPQMPTVSCQFFEPPEDRGFLPGTEAERLAEYTAVEAAVADNIHVATEDHQATGAAVLGNAICKVENEDFSCANSAAIDTDLQTESAPPVISSEFILPLPEAFEEPINLSVKKCPPPETSSVIVKKPTHLPPAPGKPLKHETEDRGFKPSFAEGRKGKENTPRTPARETRIPYVRLERLKIQAPNSGELPVFKVQPQKKDEDGSVRLVVKYGAQSKSMSIKVNPDCPYSSPHPAPQPAMPYGTEPIPYTTSQTISELEQQVNNLARVSPYSGESPAFRNRIEAPAAPIEDPEVTCNVNAAPQPTRKPVQSEDHDQLENEDFCAVCLNGGEMLCCNRCPKVFHLSWHVPALLSFPVGEWLCTLCRNLTKPEVEYDCDNVRYCLENKMEIAAFPNLDDYDQRKCETLVLSLCCNSLSLPFQEPVSPLARHYYQIIKRPMDLSIIRKKLQRRNIPHYSAPEELVYDIRLMFWNCAKFNYEDSEVAEAGRNLEMYFENMLKEAYPEKVFPFPQEEDSDTEEIGSESCHLSLKGFHWPPYGPDYLQPKRRRRHTLSQKPREFDIC

Target ORF information:

RefSeq Version	XM_002942863.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942863.4

ORF Insert Sequence:

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAATG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTAACCGTT GCCCAAAAGT CTTTCACCTT TCCTGGCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATG AAGACTCTGA AGTGGCCGAA GCCGGACGGA ACCTGGAGAT GTACTTTGAG 
AACATGCTGA AGGAGGCCTA CCCCGAGAAG GTCTTCCCTT TCCCACAGGA GGAAGATTCC 
GACACGGAGG AGATCGGCAG TGAGAGCTGC CACCTGAGCC TGAAAGGCTT TCACTGGCCA 
CCCTACGGGC CGGACTACCT GCAGCCCAAG CGTAGGAGGC GCCATACCCT TAGCCAGAAG 
CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim66 ( XM_004913327.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913384.2 Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X1, mRNA.

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CloneID	OXa56366
Clone ID Related Accession (Same CDS sequence)	XM_004913327.4
Accession Version	XM_004913327.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913327.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAAGG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTGACCGTT GCCCAAAAGT CTTTCACCTT TCCTGCCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATA TAGGGCCCGC AGAACAGAAG GCAATATTCA AGGAAGACTC TGAAGTGGCC 
GAAGCCGGAC GGAACCTGGA GATGTACTTT GAGAACATGC TGAAGGAGGC CTACCCCGAG 
AAGGTCTTCC CTTTCCCACA GGAGGAAGAT TCCGACACGG AGGAGATCGG CAGTGAGAGC 
TGCCACCTGA GCCTGAAAGG CTTTCACTGG CCACCCTACG GGCCGGACTA CCTGCAGCCC 
AAGCGTAGGA GGCGCCATAC CCTTAGCCAG AAGCCCAGGG AGTTCGACAT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913384.2
CDS	871..4527
Translation	MAKSCFDCTEKNSAHSLCITCNKWLCSTCAEQHRHTKSVSDPFIPAVQRGSAGNDGTSGNFLCCPLHHQESLKLFCETCDTLVCRHCVVMEHKDHRFRRLDEALQHQRAVLENVITQVEEKKVVVQAAGKQIEDRLYEIKHMHSKVENQIKVTKMVLINELNKRTNVLLEQLEKITTEKRHKYEQQLQSILVLNRQLEHVQNFINWAMYGKNSIPFLFSKELIVYQMQRLLDTSCGGDVGRTSKIRFSWEPSFWTKQISNLGCLMADGAHLPTPDIPTYGTENAQAPYYPAPHVPITAHPGTITSSPHYTSSVQCPTPMCCTHCHTLPTVPKNQPATGTVTPHVSFAHPTPIKQQQPPSTQYNSSKEQKPTVRPLRVIQPWVSHQPQAEQENSPYWLDHQQQKPQVPPSQPNAPPVCPVPPQDFNQVHNVHTIQHTVPITTPTSLQAPSVLQTPSVLQAPSVQMQVSSVHSLSHIQPQRLQQQSALCQAESAHEQVMHHSLDIINQQFELEQMQKGLELLLQSQPSNVQLNPTKQPQHVQQTIVGQINYIVRQPATAPLQPPEEIPQVCDEPISPGSHTVDLSPVTTSSPSNMQSQSIADEIIAAVENDACQRNQLAANPERKRSASVGFCNVPEMELSSPRLSRSVDPQMPTVSCQFFEPPEDRGFLPGTEAERLAEYTAVEAAVADNIHVATEDHQATGAAVLGNAICKVENEDFSCANSAAIDTDLQTESAPPVISSEFILPLPEAFEEPINLSVKKCPPPETSSVIVKKPTHLPPAPGKPLKHETEDRGFKPSFAEGRKGKENTPRTPARETRIPYVRLERLKIQAPNSGELPVFKVQPQKKDEDGSVRLVVKYGAQSKSMSIKVNPDCPYSSPHPAPQPAMPYGTEPIPYTTSQTISELEQQVNNLARVSPYSGESPAFRNRIEAPAAPIEDPEVTCKVNAAPQPTRKPVQSEDHDQLENEDFCAVCLNGGEMLCCDRCPKVFHLSCHVPALLSFPVGEWLCTLCRNLTKPEVEYDCDNVRYCLENKMEIAAFPNLDDYDQRKCETLVLSLCCNSLSLPFQEPVSPLARHYYQIIKRPMDLSIIRKKLQRRNIPHYSAPEELVYDIRLMFWNCAKFNYIGPAEQKAIFKEDSEVAEAGRNLEMYFENMLKEAYPEKVFPFPQEEDSDTEEIGSESCHLSLKGFHWPPYGPDYLQPKRRRRHTLSQKPREFDIC

Target ORF information:

RefSeq Version	XM_004913327.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913327.4

ORF Insert Sequence:

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAAGG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTGACCGTT GCCCAAAAGT CTTTCACCTT TCCTGCCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATA TAGGGCCCGC AGAACAGAAG GCAATATTCA AGGAAGACTC TGAAGTGGCC 
GAAGCCGGAC GGAACCTGGA GATGTACTTT GAGAACATGC TGAAGGAGGC CTACCCCGAG 
AAGGTCTTCC CTTTCCCACA GGAGGAAGAT TCCGACACGG AGGAGATCGG CAGTGAGAGC 
TGCCACCTGA GCCTGAAAGG CTTTCACTGG CCACCCTACG GGCCGGACTA CCTGCAGCCC 
AAGCGTAGGA GGCGCCATAC CCTTAGCCAG AAGCCCAGGG AGTTCGACAT CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim66 ( XM_004913328.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913385.2 Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56367
Clone ID Related Accession (Same CDS sequence)	XM_004913328.4
Accession Version	XM_004913328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tripartite motif-containing protein 66 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913328.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAC AGAGGGTTCA AGCCGAGCTT TGCAGAAGGC 
AGAAAGGGCA AGGAAAACAC ACCCAGGACG CCGGCGAGGG AAACCCGAAT CCCCTACGTG 
CGACTGGAGC GGCTAAAGAT CCAGGCTCCC AACTCCGGGG AACTCCCTGT TTTTAAGGTT 
CAGCCGCAGA AAAAAGATGA AGACGGGTCG GTGCGTTTAG TTGTCAAATA TGGCGCCCAG 
TCCAAGAGCA TGTCCATAAA GGTTAACCCA GACTGCCCGT ACTCATCCCC CCACCCCGCC 
CCGCAACCCG CAATGCCTTA TGGGACAGAA CCAATCCCTT ACACCACCAG CCAAACCATT 
TCTGAACTGG AGCAACAAGT GAACAATCTG GCGAGGGTGA GTCCCTACAG CGGGGAGAGC 
CCGGCATTCA GGAACAGAAT CGAGGCCCCG GCGGCTCCCA TAGAAGATCC TGAGGTGACG 
TGCAAGGTCA ACGCTGCCCC TCAGCCAACC AGAAAGCCCG TCCAATCAGA AGACCATGAT 
CAGCTTGAGA ATGAGGATTT CTGTGCTGTT TGTCTGAATG GAGGGGAAAT GCTTTGCTGT 
GACCGTTGCC CAAAAGTCTT TCACCTTTCC TGCCATGTTC CAGCCCTGCT CAGCTTCCCT 
GTGGGCGAAT GGCTTTGCAC CTTGTGCCGA AACCTGACCA AACCAGAGGT AGAATACGAC 
TGCGACAATG TCCGCTATTG TCTAGAGAAT AAAATGGAGA TTGCGGCTTT TCCCAACCTT 
GACGACTACG ACCAAAGGAA GTGTGAGACG CTGGTGCTGT CGCTGTGCTG TAACAGTCTG 
AGCCTCCCAT TCCAGGAACC CGTCAGCCCC TTGGCGCGGC ACTACTACCA GATCATAAAG 
CGCCCGATGG ACCTCTCCAT TATACGGAAG AAGCTCCAGA GACGCAATAT CCCGCATTAC 
TCGGCGCCGG AGGAACTGGT GTACGATATT CGCCTCATGT TCTGGAACTG CGCAAAGTTT 
AATTATATAG GGCCCGCAGA ACAGAAGGCA ATATTCAAGG AAGACTCTGA AGTGGCCGAA 
GCCGGACGGA ACCTGGAGAT GTACTTTGAG AACATGCTGA AGGAGGCCTA CCCCGAGAAG 
GTCTTCCCTT TCCCACAGGA GGAAGATTCC GACACGGAGG AGATCGGCAG TGAGAGCTGC 
CACCTGAGCC TGAAAGGCTT TCACTGGCCA CCCTACGGGC CGGACTACCT GCAGCCCAAG 
CGTAGGAGGC GCCATACCCT TAGCCAGAAG CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913385.2
CDS	872..4525
Translation	MAKSCFDCTEKNSAHSLCITCNKWLCSTCAEQHRHTKSVSDPFIPAVQRGSAGNDGTSGNFLCCPLHHQESLKLFCETCDTLVCRHCVVMEHKDHRFRRLDEALQHQRAVLENVITQVEEKKVVVQAAGKQIEDRLYEIKHMHSKVENQIKVTKMVLINELNKRTNVLLEQLEKITTEKRHKYEQQLQSILVLNRQLEHVQNFINWAMYGKNSIPFLFSKELIVYQMQRLLDTSCGGDVGRTSKIRFSWEPSFWTKQISNLGCLMADGAHLPTPDIPTYGTENAQAPYYPAPHVPITAHPGTITSSPHYTSSVQCPTPMCCTHCHTLPTVPKNQPATGTVTPHVSFAHPTPIKQQQPPSTQYNSSKEQKPTVRPLRVIQPWVSHQPQAEQENSPYWLDHQQQKPQVPPSQPNAPPVCPVPPQDFNQVHNVHTIQHTVPITTPTSLQAPSVLQTPSVLQAPSVQMQVSSVHSLSHIQPQRLQQQSALCQAESAHEQVMHHSLDIINQQFELEQMQKGLELLLQSQPSNVQLNPTKQPQHVQQTIVGQINYIVRQPATAPLQPPEEIPQVCDEPISPGSHTVDLSPVTTSSPSNMQSQSIADEIIAAVENDACQRNQLAANPERKRSASVGFCNVPEMELSSPRLSRSVDPQMPTVSCQFFEPPEDRGFLPGTEAERLAEYTAVEAAVADNIHVATEDHQATGAAVLGNAICKVENEDFSCANSAAIDTDLQTESAPPVISSEFILPLPEAFEEPINLSVKKCPPPETSSVIVKKPTHLPPAPGKPLKHETDRGFKPSFAEGRKGKENTPRTPARETRIPYVRLERLKIQAPNSGELPVFKVQPQKKDEDGSVRLVVKYGAQSKSMSIKVNPDCPYSSPHPAPQPAMPYGTEPIPYTTSQTISELEQQVNNLARVSPYSGESPAFRNRIEAPAAPIEDPEVTCKVNAAPQPTRKPVQSEDHDQLENEDFCAVCLNGGEMLCCDRCPKVFHLSCHVPALLSFPVGEWLCTLCRNLTKPEVEYDCDNVRYCLENKMEIAAFPNLDDYDQRKCETLVLSLCCNSLSLPFQEPVSPLARHYYQIIKRPMDLSIIRKKLQRRNIPHYSAPEELVYDIRLMFWNCAKFNYIGPAEQKAIFKEDSEVAEAGRNLEMYFENMLKEAYPEKVFPFPQEEDSDTEEIGSESCHLSLKGFHWPPYGPDYLQPKRRRRHTLSQKPREFDIC

Target ORF information:

RefSeq Version	XM_004913328.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913328.4

ORF Insert Sequence:

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAC AGAGGGTTCA AGCCGAGCTT TGCAGAAGGC 
AGAAAGGGCA AGGAAAACAC ACCCAGGACG CCGGCGAGGG AAACCCGAAT CCCCTACGTG 
CGACTGGAGC GGCTAAAGAT CCAGGCTCCC AACTCCGGGG AACTCCCTGT TTTTAAGGTT 
CAGCCGCAGA AAAAAGATGA AGACGGGTCG GTGCGTTTAG TTGTCAAATA TGGCGCCCAG 
TCCAAGAGCA TGTCCATAAA GGTTAACCCA GACTGCCCGT ACTCATCCCC CCACCCCGCC 
CCGCAACCCG CAATGCCTTA TGGGACAGAA CCAATCCCTT ACACCACCAG CCAAACCATT 
TCTGAACTGG AGCAACAAGT GAACAATCTG GCGAGGGTGA GTCCCTACAG CGGGGAGAGC 
CCGGCATTCA GGAACAGAAT CGAGGCCCCG GCGGCTCCCA TAGAAGATCC TGAGGTGACG 
TGCAAGGTCA ACGCTGCCCC TCAGCCAACC AGAAAGCCCG TCCAATCAGA AGACCATGAT 
CAGCTTGAGA ATGAGGATTT CTGTGCTGTT TGTCTGAATG GAGGGGAAAT GCTTTGCTGT 
GACCGTTGCC CAAAAGTCTT TCACCTTTCC TGCCATGTTC CAGCCCTGCT CAGCTTCCCT 
GTGGGCGAAT GGCTTTGCAC CTTGTGCCGA AACCTGACCA AACCAGAGGT AGAATACGAC 
TGCGACAATG TCCGCTATTG TCTAGAGAAT AAAATGGAGA TTGCGGCTTT TCCCAACCTT 
GACGACTACG ACCAAAGGAA GTGTGAGACG CTGGTGCTGT CGCTGTGCTG TAACAGTCTG 
AGCCTCCCAT TCCAGGAACC CGTCAGCCCC TTGGCGCGGC ACTACTACCA GATCATAAAG 
CGCCCGATGG ACCTCTCCAT TATACGGAAG AAGCTCCAGA GACGCAATAT CCCGCATTAC 
TCGGCGCCGG AGGAACTGGT GTACGATATT CGCCTCATGT TCTGGAACTG CGCAAAGTTT 
AATTATATAG GGCCCGCAGA ACAGAAGGCA ATATTCAAGG AAGACTCTGA AGTGGCCGAA 
GCCGGACGGA ACCTGGAGAT GTACTTTGAG AACATGCTGA AGGAGGCCTA CCCCGAGAAG 
GTCTTCCCTT TCCCACAGGA GGAAGATTCC GACACGGAGG AGATCGGCAG TGAGAGCTGC 
CACCTGAGCC TGAAAGGCTT TCACTGGCCA CCCTACGGGC CGGACTACCT GCAGCCCAAG 
CGTAGGAGGC GCCATACCCT TAGCCAGAAG CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trim66 ( XM_002942863.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942909.3 Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X3, mRNA.

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CloneID	OXa56368
Clone ID Related Accession (Same CDS sequence)	XM_002942863.5
Accession Version	XM_002942863.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tripartite motif-containing protein 66 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942863.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAAGG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTGACCGTT GCCCAAAAGT CTTTCACCTT TCCTGCCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATG AAGACTCTGA AGTGGCCGAA GCCGGACGGA ACCTGGAGAT GTACTTTGAG 
AACATGCTGA AGGAGGCCTA CCCCGAGAAG GTCTTCCCTT TCCCACAGGA GGAAGATTCC 
GACACGGAGG AGATCGGCAG TGAGAGCTGC CACCTGAGCC TGAAAGGCTT TCACTGGCCA 
CCCTACGGGC CGGACTACCT GCAGCCCAAG CGTAGGAGGC GCCATACCCT TAGCCAGAAG 
CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942909.3
CDS	871..4494
Translation	MAKSCFDCTEKNSAHSLCITCNKWLCSTCAEQHRHTKSVSDPFIPAVQRGSAGNDGTSGNFLCCPLHHQESLKLFCETCDTLVCRHCVVMEHKDHRFRRLDEALQHQRAVLENVITQVEEKKVVVQAAGKQIEDRLYEIKHMHSKVENQIKVTKMVLINELNKRTNVLLEQLEKITTEKRHKYEQQLQSILVLNRQLEHVQNFINWAMYGKNSIPFLFSKELIVYQMQRLLDTSCGGDVGRTSKIRFSWEPSFWTKQISNLGCLMADGAHLPTPDIPTYGTENAQAPYYPAPHVPITAHPGTITSSPHYTSSVQCPTPMCCTHCHTLPTVPKNQPATGTVTPHVSFAHPTPIKQQQPPSTQYNSSKEQKPTVRPLRVIQPWVSHQPQAEQENSPYWLDHQQQKPQVPPSQPNAPPVCPVPPQDFNQVHNVHTIQHTVPITTPTSLQAPSVLQTPSVLQAPSVQMQVSSVHSLSHIQPQRLQQQSALCQAESAHEQVMHHSLDIINQQFELEQMQKGLELLLQSQPSNVQLNPTKQPQHVQQTIVGQINYIVRQPATAPLQPPEEIPQVCDEPISPGSHTVDLSPVTTSSPSNMQSQSIADEIIAAVENDACQRNQLAANPERKRSASVGFCNVPEMELSSPRLSRSVDPQMPTVSCQFFEPPEDRGFLPGTEAERLAEYTAVEAAVADNIHVATEDHQATGAAVLGNAICKVENEDFSCANSAAIDTDLQTESAPPVISSEFILPLPEAFEEPINLSVKKCPPPETSSVIVKKPTHLPPAPGKPLKHETEDRGFKPSFAEGRKGKENTPRTPARETRIPYVRLERLKIQAPNSGELPVFKVQPQKKDEDGSVRLVVKYGAQSKSMSIKVNPDCPYSSPHPAPQPAMPYGTEPIPYTTSQTISELEQQVNNLARVSPYSGESPAFRNRIEAPAAPIEDPEVTCKVNAAPQPTRKPVQSEDHDQLENEDFCAVCLNGGEMLCCDRCPKVFHLSCHVPALLSFPVGEWLCTLCRNLTKPEVEYDCDNVRYCLENKMEIAAFPNLDDYDQRKCETLVLSLCCNSLSLPFQEPVSPLARHYYQIIKRPMDLSIIRKKLQRRNIPHYSAPEELVYDIRLMFWNCAKFNYEDSEVAEAGRNLEMYFENMLKEAYPEKVFPFPQEEDSDTEEIGSESCHLSLKGFHWPPYGPDYLQPKRRRRHTLSQKPREFDIC

Target ORF information:

RefSeq Version	XM_002942863.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tripartite motif containing 66 (trim66), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942863.5

ORF Insert Sequence:

ATGGCCAAGA GCTGCTTTGA TTGTACAGAG AAAAACTCCG CCCACAGTCT GTGCATCACA 
TGCAACAAAT GGCTTTGCAG CACCTGCGCC GAGCAACACC GGCACACCAA GAGTGTCAGC 
GACCCCTTCA TACCCGCTGT CCAGAGGGGC TCCGCAGGCA ATGATGGCAC CTCGGGAAAT 
TTCCTTTGCT GCCCGCTGCA CCACCAAGAG TCCCTGAAGT TATTCTGCGA GACCTGCGAT 
ACCTTGGTTT GCCGCCATTG TGTCGTCATG GAGCACAAAG ACCACAGATT CCGAAGGCTG 
GATGAGGCCT TGCAACACCA GAGAGCCGTC CTGGAGAATG TTATAACCCA AGTGGAGGAG 
AAGAAGGTCG TGGTTCAGGC AGCAGGCAAA CAAATTGAGG ACAGGTTGTA TGAAATTAAG 
CACATGCACA GTAAAGTAGA AAACCAGATC AAAGTGACCA AAATGGTGCT GATCAATGAG 
CTGAACAAGA GGACAAATGT ACTGCTGGAA CAGCTGGAGA AAATCACAAC AGAAAAGCGC 
CACAAATACG AGCAGCAGCT TCAGTCGATA CTGGTCCTGA ATCGCCAGTT GGAGCACGTG 
CAGAACTTTA TTAACTGGGC CATGTATGGA AAGAACAGTA TCCCGTTCCT GTTCAGTAAG 
GAACTGATTG TTTATCAGAT GCAGCGGCTC CTGGACACCA GCTGTGGGGG GGACGTGGGG 
CGCACGTCGA AGATCCGGTT CAGTTGGGAG CCATCTTTCT GGACCAAACA AATATCCAAT 
TTGGGCTGCC TAATGGCGGA TGGAGCCCAC TTGCCAACGC CGGACATACC CACCTACGGA 
ACAGAAAATG CCCAAGCCCC GTATTACCCA GCACCCCATG TACCCATTAC AGCCCACCCT 
GGTACCATCA CCAGTTCCCC ACATTACACA TCTTCAGTCC AGTGTCCCAC GCCCATGTGT 
TGCACCCATT GCCATACCTT GCCAACCGTG CCAAAGAACC AGCCGGCCAC GGGCACAGTG 
ACCCCCCACG TGAGCTTCGC CCACCCAACG CCAATCAAAC AACAGCAGCC CCCATCTACC 
CAGTATAACT CCAGCAAAGA GCAAAAGCCA ACCGTCCGTC CTTTGAGGGT CATCCAGCCT 
TGGGTATCCC ACCAGCCTCA GGCGGAACAG GAGAACTCCC CCTACTGGCT GGACCATCAG 
CAGCAGAAGC CACAGGTGCC ACCGAGCCAA CCAAATGCCC CACCTGTTTG CCCAGTGCCC 
CCACAGGATT TCAACCAAGT GCACAATGTG CATACGATCC AACATACGGT GCCCATCACC 
ACGCCAACCT CTCTGCAGGC ACCATCAGTA CTTCAGACGC CCTCGGTGCT CCAGGCACCA 
TCGGTACAAA TGCAAGTGAG CTCGGTGCAC AGCCTTAGCC ACATCCAACC CCAGAGGCTC 
CAGCAGCAAT CGGCCCTGTG CCAGGCGGAG AGCGCCCACG AGCAGGTCAT GCACCACAGC 
CTGGACATTA TTAACCAACA GTTTGAGTTG GAGCAGATGC AGAAGGGCCT GGAACTTCTT 
CTCCAAAGTC AACCGTCCAA CGTCCAACTG AACCCGACCA AGCAGCCTCA GCACGTGCAA 
CAGACTATTG TGGGGCAAAT CAACTACATA GTCAGGCAGC CGGCAACAGC CCCACTTCAG 
CCCCCGGAGG AAATTCCACA GGTCTGCGAT GAGCCCATAT CTCCGGGCAG TCACACCGTG 
GACCTCTCGC CGGTCACCAC CAGTTCTCCC AGTAACATGC AGTCCCAGTC TATAGCAGAT 
GAAATCATCG CTGCCGTGGA AAATGACGCG TGCCAGAGGA ACCAACTGGC GGCCAATCCC 
GAGAGGAAGA GATCGGCCTC GGTGGGTTTC TGTAACGTGC CAGAGATGGA ACTGTCTTCT 
CCGAGACTCT CTAGGTCTGT GGATCCTCAG ATGCCAACTG TCTCCTGTCA GTTCTTTGAG 
CCGCCGGAGG ACAGGGGGTT CCTGCCCGGC ACAGAGGCGG AGAGGTTGGC AGAGTACACG 
GCAGTGGAAG CAGCTGTCGC AGACAATATA CACGTAGCAA CCGAGGATCA CCAGGCAACC 
GGTGCCGCTG TCTTGGGAAA TGCCATATGC AAGGTTGAGA ATGAAGATTT TAGCTGCGCT 
AACAGTGCCG CCATAGACAC TGATTTGCAG ACAGAAAGTG CCCCTCCAGT AATTTCCAGC 
GAATTCATCC TGCCGCTGCC CGAGGCATTT GAGGAGCCAA TCAACCTCTC GGTGAAGAAA 
TGCCCCCCCC CAGAAACCTC CTCGGTGATT GTGAAAAAGC CCACTCACCT TCCTCCTGCC 
CCCGGCAAAC CACTCAAACA TGAGACAGAA GACAGAGGGT TCAAGCCGAG CTTTGCAGAA 
GGCAGAAAGG GCAAGGAAAA CACACCCAGG ACGCCGGCGA GGGAAACCCG AATCCCCTAC 
GTGCGACTGG AGCGGCTAAA GATCCAGGCT CCCAACTCCG GGGAACTCCC TGTTTTTAAG 
GTTCAGCCGC AGAAAAAAGA TGAAGACGGG TCGGTGCGTT TAGTTGTCAA ATATGGCGCC 
CAGTCCAAGA GCATGTCCAT AAAGGTTAAC CCAGACTGCC CGTACTCATC CCCCCACCCC 
GCCCCGCAAC CCGCAATGCC TTATGGGACA GAACCAATCC CTTACACCAC CAGCCAAACC 
ATTTCTGAAC TGGAGCAACA AGTGAACAAT CTGGCGAGGG TGAGTCCCTA CAGCGGGGAG 
AGCCCGGCAT TCAGGAACAG AATCGAGGCC CCGGCGGCTC CCATAGAAGA TCCTGAGGTG 
ACGTGCAAGG TCAACGCTGC CCCTCAGCCA ACCAGAAAGC CCGTCCAATC AGAAGACCAT 
GATCAGCTTG AGAATGAGGA TTTCTGTGCT GTTTGTCTGA ATGGAGGGGA AATGCTTTGC 
TGTGACCGTT GCCCAAAAGT CTTTCACCTT TCCTGCCATG TTCCAGCCCT GCTCAGCTTC 
CCTGTGGGCG AATGGCTTTG CACCTTGTGC CGAAACCTGA CCAAACCAGA GGTAGAATAC 
GACTGCGACA ATGTCCGCTA TTGTCTAGAG AATAAAATGG AGATTGCGGC TTTTCCCAAC 
CTTGACGACT ACGACCAAAG GAAGTGTGAG ACGCTGGTGC TGTCGCTGTG CTGTAACAGT 
CTGAGCCTCC CATTCCAGGA ACCCGTCAGC CCCTTGGCGC GGCACTACTA CCAGATCATA 
AAGCGCCCGA TGGACCTCTC CATTATACGG AAGAAGCTCC AGAGACGCAA TATCCCGCAT 
TACTCGGCGC CGGAGGAACT GGTGTACGAT ATTCGCCTCA TGTTCTGGAA CTGCGCAAAG 
TTTAATTATG AAGACTCTGA AGTGGCCGAA GCCGGACGGA ACCTGGAGAT GTACTTTGAG 
AACATGCTGA AGGAGGCCTA CCCCGAGAAG GTCTTCCCTT TCCCACAGGA GGAAGATTCC 
GACACGGAGG AGATCGGCAG TGAGAGCTGC CACCTGAGCC TGAAAGGCTT TCACTGGCCA 
CCCTACGGGC CGGACTACCT GCAGCCCAAG CGTAGGAGGC GCCATACCCT TAGCCAGAAG 
CCCAGGGAGT TCGACATCTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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trim66 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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会社情報