TRIM66 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014122779.1	XP_013978254.1	tripartite motif-containing protein 66 isoform X2
XM_014122784.1	XP_013978259.1	tripartite motif-containing protein 66 isoform X4
XM_014122779.2	XP_013978254.1	tripartite motif-containing protein 66 isoform X3
XM_014122783.2	XP_013978258.1	tripartite motif-containing protein 66 isoform X5
XM_022407811.1	XP_022263519.1	tripartite motif-containing protein 66 isoform X2
XM_005633636.2	XP_005633693.1	tripartite motif-containing protein 66 isoform X1
XM_014122782.2	XP_013978257.1	tripartite motif-containing protein 66 isoform X5
XM_014122780.1	XP_013978255.1	tripartite motif-containing protein 66 isoform X4
XM_014122781.1	XP_013978256.1	tripartite motif-containing protein 66 isoform X4
XM_014122783.1	XP_013978258.1	tripartite motif-containing protein 66 isoform X4
XM_014122780.2	XP_013978255.1	tripartite motif-containing protein 66 isoform X5
XM_022407812.1	XP_022263520.1	tripartite motif-containing protein 66 isoform X5
XM_005633637.2	XP_005633694.1	tripartite motif-containing protein 66 isoform X3
XM_014122782.1	XP_013978257.1	tripartite motif-containing protein 66 isoform X4
XM_005633636.3	XP_005633693.1	tripartite motif-containing protein 66 isoform X1
XM_005633637.3	XP_005633694.1	tripartite motif-containing protein 66 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe296054	XM_022407811.1 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe42226	XM_014122779.2 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe42227	XM_014122781.1 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_022407812.1 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_014122780.2 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_014122782.2 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_014122783.2 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_014122784.1 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe23168	XM_005633636.3 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe23169	XM_005633637.3 Latest version!	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe42226	XM_014122779.1	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe42227	XM_014122783.1	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_014122780.1	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42227	XM_014122782.1	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe23168	XM_005633636.2	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe23169	XM_005633637.2	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

TRIM66 ( XM_022407811.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263519.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe296054
Clone ID Related Accession (Same CDS sequence)	XM_022407811.1
Accession Version	XM_022407811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATGC AGCGATTGCT GGAGGCAAAC 
TGTAACACAG ATCCGGGCTC CCCCTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TGGCTTCCCT TGGCTGTATA ACTACTGAAG GTGGACAGCT GTCCCGGGCA 
GATACTCCTG CTTATGGAGG CTTACAGGGA CCATCATCCT TTTATCAAAG CCACCAGTCC 
CCAGTGGCTC AGCAAGAGAC TCTTAGCCAC CCCTCACATA AGTTCCAGTC TCCAGCACTG 
TGTTCTTCAT CTGTATGCTG CTCCCACTGC TCCCCAGCCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CTCAGCCCAC AGCTTTAGGC AGCCCTCAGA GATGGCACCC 
CAACAGCTAG GCTCTCTGCA GTGCTCTACT CTGCTGCCCA GGGAGAAGGA GCTGCCTTGC 
AATCCTCAGC CACCAAAACT GATGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAACACAT CCCAGCGGCT GGGGCAACAG CCAACTTCCC AGCCAGTATG CATTGTGCCC 
CCGCAGGATG TTCAGCAAGG AGCCCATGCC CAACCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGCCACT TTCAGCAGCA GCCACAGCAG 
CAACTGCCAC CTCCGCCACC TCTGCCACCC CCACCCCAGC AGCAGCCACT GCCACCTCTA 
CCTCCACCCC AGCATCCAGC TTCTAGTCAG CATGGGAGCC CTCCTGGGCC TGCCTGTTCC 
CAGAACATGG ATATAATGCA CCACAAATTT GAGCTGGAGG AGATGCAGAA GGACCTGGAG 
CTTCTTCTTC AGGCTCAACA GCCCAGCCTA CAGCTGAATC AGACCAAATC TCCTCAGCAT 
CTTCAGCAAA CCATTGTGGG ACAGATCAAC TACATTGTGA GGCAGCCAGC ACCCGTCCAG 
TCCCAGAGCC AAGAGGATGC TGCACAGGCT TCGGATGAGC ACCCCACATC TGAAGGTCCA 
AAGCCGCCTC TCCCACTTGA CAAGAATACT GCTGCTAACT TGCCCCAGGC GTCTGGGGAG 
GAAACCCCTC CTGGTGTCCC CCCACCAGAC GGCACCATCC CGCACTCCTC TCCAAGTGTG 
GTGAGAAAGC ACTCCACTTC AGTGAGCATC ATGGGCTTCT CCAACACGCT AGAGATGGAG 
CTGTCATCTA CCAGGCTAGC CAGGACCCTG GAGCCGCAGA TCCAGAGCGT GAGCAGCCTG 
ACTGCTGCCC CTTCCCACGT GGTCCCAAGC CTGCTGAGCG GCACCCCCAC AACTGTGTCC 
AGCCTGCTGA GCGGTCAGAG CCAGGCTGTG CCCAGCCTGA CAGTTAGTCC TCTGCAGACC 
ATACCCAGTC TTGTACGTGG CACACCCCGG ACCGTGCCCG GTTTGATGAG TGATTCCTCC 
CAGGCCATCA CAAGCCTAGC AAGTGATCAC TCACAGGCCA GGCCCAAGCT GATGTCTGGT 
CCCACCCAGA CTCTGCCAAG TCTGGCTACT TGTCCACTGC AGAGCATGCC TCCGGCTTCT 
GACACGCAAC CGGAAACTGC ATCCAGTGGC AGTCCTGGCT CTGGCGGAGC TGTGGGGAGC 
CTGTGCCCTG GAGATGGGGC TGACCCCTCT CTTGGGAATG CCCTGTGTAA GATGGAAAGT 
GAGGATTCTT CCCGCTTCAC TGACTCTCTG GAACAGGACC CCACAACTCC TGGTCTGGAG 
GTTTCCAAGG ATTTGGCTAT CCCTTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAG TGGTCCCAGC AGTCAGCACA GCTATGGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTATG AGAATTTTGA AGAAGCCCTG GAGCTGAATG TAAAAGAGAA CCAGAGTATC 
AGGCGAGAGC CTAAGATTCC CTATGTGCGG CTGGAGCGGC TTAAGATCTG TGCTGCCTCC 
TCGGGAGAGA TGCCTGTGTT CAAGCTGAAG CCCCAGAAGA ATGAGCAGGA TGGGAGCTTC 
CTGCTGATCA TCGAGTGTGG CACAGAGTCC TCCAGCATGT CCATTAAGGT CAGCCAGAAC 
AACCTGCCTG ATGCCATCCA GGCCCCAAGT CTGGGGGGAA GAAAGGTCAC TGTCACTTCT 
CTGGCTGGAC AACAGCCACC AGAAGGGGAG GGTGCATCTC CTGAAGAACA AAGGCTCATC 
CCTCGGACCC TTGGAGCCAA GAAGGGGCCC CCAGTACCAA TAGAGAATGA GGACTTCTGT 
GCTGTGTGCC TCAATGGGGG CGAGCTACTG TGCTGTGACC GCTGCCCTAA GGTGTTCCAC 
CTCTCCTGCC ACCTGCCGGC CTTGCTTGGC TTCCCAGGGG GAGATTGGGT GTGTACCTTG 
TGCCGCAGCC TGACCCAGCC CGAGATGGAG TATGACTGTG AGAATGCCCG CTATTACCAA 
CCTGGGATGC GGGACACCCC TGGCCTGAGC ATCTATGACC AAAAGAAGTG TGAAAAGTTG 
GTTCTGTCCT TGTGCTGCAA TAGCCTCAGC CTGCCCTTCC ACGAGCCTGT CAGCCCGCTG 
GCCCGGCATT ACTACCAGAT TATCAAGAGG CCCATGGACC TGTCAATCAT CCGGAGGAAG 
CTGCAAAAGA AGGACCCAGC TCACTACACC ACCCCAGAGG AGGTGGTGTC AGACGTCCGC 
CTCATGTTCT GGAACTGTGC TAAATTCAAT TATCCTGACT CAGAAGTTGC AGAGGCTGGC 
CGCTGCCTGG AAGTTTTCTT TGAGGGCTGG TTGAAGGAGA TCTATCCAGA AAAACAGTTT 
GCCCAGCCGA GGCAGGAGGA CTCAGACTCC GAGGAGGTGT CTAGCGAGAG TGGCTATTCC 
ACTCCCCAGG GCTTCCCATG GCCTCCCTAT GTGCAGGAGG GCATCCAACC CAAGAGGCGG 
CGGCGACATA TGGAGAATGA AAGAGCAAAA AGAATGTCAT TCCGCTTGGC CAGCAGCGTC 
TCCCAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263519.1
CDS	94..4245
Translation	MSRREMISGSNLARQGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_022407811.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407811.1

ORF Insert Sequence:

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATGC AGCGATTGCT GGAGGCAAAC 
TGTAACACAG ATCCGGGCTC CCCCTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TGGCTTCCCT TGGCTGTATA ACTACTGAAG GTGGACAGCT GTCCCGGGCA 
GATACTCCTG CTTATGGAGG CTTACAGGGA CCATCATCCT TTTATCAAAG CCACCAGTCC 
CCAGTGGCTC AGCAAGAGAC TCTTAGCCAC CCCTCACATA AGTTCCAGTC TCCAGCACTG 
TGTTCTTCAT CTGTATGCTG CTCCCACTGC TCCCCAGCCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CTCAGCCCAC AGCTTTAGGC AGCCCTCAGA GATGGCACCC 
CAACAGCTAG GCTCTCTGCA GTGCTCTACT CTGCTGCCCA GGGAGAAGGA GCTGCCTTGC 
AATCCTCAGC CACCAAAACT GATGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAACACAT CCCAGCGGCT GGGGCAACAG CCAACTTCCC AGCCAGTATG CATTGTGCCC 
CCGCAGGATG TTCAGCAAGG AGCCCATGCC CAACCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGCCACT TTCAGCAGCA GCCACAGCAG 
CAACTGCCAC CTCCGCCACC TCTGCCACCC CCACCCCAGC AGCAGCCACT GCCACCTCTA 
CCTCCACCCC AGCATCCAGC TTCTAGTCAG CATGGGAGCC CTCCTGGGCC TGCCTGTTCC 
CAGAACATGG ATATAATGCA CCACAAATTT GAGCTGGAGG AGATGCAGAA GGACCTGGAG 
CTTCTTCTTC AGGCTCAACA GCCCAGCCTA CAGCTGAATC AGACCAAATC TCCTCAGCAT 
CTTCAGCAAA CCATTGTGGG ACAGATCAAC TACATTGTGA GGCAGCCAGC ACCCGTCCAG 
TCCCAGAGCC AAGAGGATGC TGCACAGGCT TCGGATGAGC ACCCCACATC TGAAGGTCCA 
AAGCCGCCTC TCCCACTTGA CAAGAATACT GCTGCTAACT TGCCCCAGGC GTCTGGGGAG 
GAAACCCCTC CTGGTGTCCC CCCACCAGAC GGCACCATCC CGCACTCCTC TCCAAGTGTG 
GTGAGAAAGC ACTCCACTTC AGTGAGCATC ATGGGCTTCT CCAACACGCT AGAGATGGAG 
CTGTCATCTA CCAGGCTAGC CAGGACCCTG GAGCCGCAGA TCCAGAGCGT GAGCAGCCTG 
ACTGCTGCCC CTTCCCACGT GGTCCCAAGC CTGCTGAGCG GCACCCCCAC AACTGTGTCC 
AGCCTGCTGA GCGGTCAGAG CCAGGCTGTG CCCAGCCTGA CAGTTAGTCC TCTGCAGACC 
ATACCCAGTC TTGTACGTGG CACACCCCGG ACCGTGCCCG GTTTGATGAG TGATTCCTCC 
CAGGCCATCA CAAGCCTAGC AAGTGATCAC TCACAGGCCA GGCCCAAGCT GATGTCTGGT 
CCCACCCAGA CTCTGCCAAG TCTGGCTACT TGTCCACTGC AGAGCATGCC TCCGGCTTCT 
GACACGCAAC CGGAAACTGC ATCCAGTGGC AGTCCTGGCT CTGGCGGAGC TGTGGGGAGC 
CTGTGCCCTG GAGATGGGGC TGACCCCTCT CTTGGGAATG CCCTGTGTAA GATGGAAAGT 
GAGGATTCTT CCCGCTTCAC TGACTCTCTG GAACAGGACC CCACAACTCC TGGTCTGGAG 
GTTTCCAAGG ATTTGGCTAT CCCTTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAG TGGTCCCAGC AGTCAGCACA GCTATGGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTATG AGAATTTTGA AGAAGCCCTG GAGCTGAATG TAAAAGAGAA CCAGAGTATC 
AGGCGAGAGC CTAAGATTCC CTATGTGCGG CTGGAGCGGC TTAAGATCTG TGCTGCCTCC 
TCGGGAGAGA TGCCTGTGTT CAAGCTGAAG CCCCAGAAGA ATGAGCAGGA TGGGAGCTTC 
CTGCTGATCA TCGAGTGTGG CACAGAGTCC TCCAGCATGT CCATTAAGGT CAGCCAGAAC 
AACCTGCCTG ATGCCATCCA GGCCCCAAGT CTGGGGGGAA GAAAGGTCAC TGTCACTTCT 
CTGGCTGGAC AACAGCCACC AGAAGGGGAG GGTGCATCTC CTGAAGAACA AAGGCTCATC 
CCTCGGACCC TTGGAGCCAA GAAGGGGCCC CCAGTACCAA TAGAGAATGA GGACTTCTGT 
GCTGTGTGCC TCAATGGGGG CGAGCTACTG TGCTGTGACC GCTGCCCTAA GGTGTTCCAC 
CTCTCCTGCC ACCTGCCGGC CTTGCTTGGC TTCCCAGGGG GAGATTGGGT GTGTACCTTG 
TGCCGCAGCC TGACCCAGCC CGAGATGGAG TATGACTGTG AGAATGCCCG CTATTACCAA 
CCTGGGATGC GGGACACCCC TGGCCTGAGC ATCTATGACC AAAAGAAGTG TGAAAAGTTG 
GTTCTGTCCT TGTGCTGCAA TAGCCTCAGC CTGCCCTTCC ACGAGCCTGT CAGCCCGCTG 
GCCCGGCATT ACTACCAGAT TATCAAGAGG CCCATGGACC TGTCAATCAT CCGGAGGAAG 
CTGCAAAAGA AGGACCCAGC TCACTACACC ACCCCAGAGG AGGTGGTGTC AGACGTCCGC 
CTCATGTTCT GGAACTGTGC TAAATTCAAT TATCCTGACT CAGAAGTTGC AGAGGCTGGC 
CGCTGCCTGG AAGTTTTCTT TGAGGGCTGG TTGAAGGAGA TCTATCCAGA AAAACAGTTT 
GCCCAGCCGA GGCAGGAGGA CTCAGACTCC GAGGAGGTGT CTAGCGAGAG TGGCTATTCC 
ACTCCCCAGG GCTTCCCATG GCCTCCCTAT GTGCAGGAGG GCATCCAACC CAAGAGGCGG 
CGGCGACATA TGGAGAATGA AAGAGCAAAA AGAATGTCAT TCCGCTTGGC CAGCAGCGTC 
TCCCAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122779.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978254.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe42226
Clone ID Related Accession (Same CDS sequence)	XM_014122779.1 , XM_014122779.2
Accession Version	XM_014122779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG 
CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA 
TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC 
TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG 
ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT 
GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC 
TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG 
CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT 
CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC 
TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG 
TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT 
TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT 
ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC 
AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA 
CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG 
GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC 
ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT 
GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG 
AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT 
ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG 
GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC 
CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC 
TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC 
CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT 
ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG 
CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA 
CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT 
GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG 
AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA 
CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT 
CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA 
TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC 
CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC 
AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG 
GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT 
ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA 
GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC 
ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC 
CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA 
AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT 
GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC 
CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT 
CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT 
ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT 
GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC 
TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT 
CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA 
GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC 
ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC 
CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG 
CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG 
AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG 
TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA 
AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG 
GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG 
CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA 
CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT 
GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG 
GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG 
AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC 
AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG 
AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC 
ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC 
AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC 
TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC 
TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC 
TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA 
AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978254.1
CDS	137..4261
Translation	MEGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122779.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122779.1

ORF Insert Sequence:

ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG 
CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA 
TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC 
TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG 
ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT 
GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC 
TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG 
CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT 
CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC 
TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG 
TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT 
TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT 
ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC 
AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA 
CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG 
GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC 
ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT 
GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG 
AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT 
ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG 
GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC 
CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC 
TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC 
CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT 
ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG 
CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA 
CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT 
GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG 
AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA 
CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT 
CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA 
TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC 
CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC 
AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG 
GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT 
ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA 
GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC 
ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC 
CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA 
AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT 
GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC 
CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT 
CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT 
ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT 
GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC 
TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT 
CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA 
GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC 
ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC 
CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG 
CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG 
AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG 
TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA 
AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG 
GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG 
CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA 
CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT 
GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG 
GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG 
AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC 
AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG 
AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC 
ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC 
AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC 
TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC 
TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC 
TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA 
AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122779.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978254.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

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CloneID	OCe42226
Clone ID Related Accession (Same CDS sequence)	XM_014122779.1 , XM_014122779.2
Accession Version	XM_014122779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG 
CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA 
TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC 
TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG 
ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT 
GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC 
TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG 
CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT 
CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC 
TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG 
TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT 
TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT 
ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC 
AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA 
CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG 
GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC 
ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT 
GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG 
AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT 
ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG 
GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC 
CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC 
TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC 
CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT 
ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG 
CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA 
CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT 
GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG 
AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA 
CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT 
CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA 
TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC 
CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC 
AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG 
GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT 
ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA 
GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC 
ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC 
CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA 
AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT 
GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC 
CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT 
CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT 
ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT 
GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC 
TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT 
CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA 
GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC 
ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC 
CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG 
CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG 
AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG 
TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA 
AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG 
GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG 
CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA 
CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT 
GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG 
GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG 
AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC 
AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG 
AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC 
ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC 
AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC 
TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC 
TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC 
TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA 
AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978254.1
CDS	185..4309
Translation	MEGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122779.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122779.2

ORF Insert Sequence:

ATGGAGGGAG TGGAGCTTGC TCGATCTACA CGCTGCTGCA CACTTCAGGA TATCATGGGA 
AGAGCCCCAG TCCCGGTCAC AAGCACAGCC ACGGATACGG GCTTGAGATT TATGGGGCTG 
CCACTAGTTG ACCAGAAACA CAGGAATGGT CAGATGGAGG CCATGATATT GACCTGCTCA 
TTGTGCCAAC AAGACCTGCA AAATGTGGGC GCCCGGTTCC TACCCTGCCA GCATTTGCTC 
TGCAAGAACT GCTTCCAGGG CTTCATCCAG GAGCTGAGAC AGGTTTCCAA AGCTCACAGG 
ACCGTTCCAC CTGAGCTCAT CTCCTGTCCT GGGTGTAAGC GAGTATATCT TACCAGGGAT 
GTAAATGAAC ATTTTTTTCT GCAGTGCTTT TCTACAGGGC GATCCAAGAT GGCCAGGAAC 
TGCTCCGAGT GCAAGGAGAA GAGGGCAGCG CATATCCTGT GCACCTACTG CAACCGCTGG 
CTGTGCAGCT CATGCACAGA GGAGCACCGG CATGGCCCCG CCCCTGGGGG CCCACTCTTT 
CCGCGGGCCC ACAAGGGACC ACCAGGAGTG AGCAGTGGTT CTGGGGACTT CGCCTTGTAC 
TGTCCTCTAC ACACACAGGA AGTGCTCAAG CTATTCTGCG AGACCTGTGA TGTGCTCACG 
TGCCATAGCT GCCTCATGGT GGAACACAAA GAACACAGGT GTAGACATCT TGAAGAAGTT 
TTGCAAAACC AGAGGATGCT TCTAGAAAGT GTGACTACAC AGGTGGCTCA TAAGAAATCT 
ACTCTACAGA CATCTGCAAA GCAAATTGAG GACAGGATTT TTGAGGTGAA GCATCAACAC 
AGGAAGGTGG AAAACCAGAT CAAGATGGCC AAGATGGTTC TGATGAATGA GCTGAACAAA 
CAGGCCAACG GGCTCATAGA GGAGTTGGAG GGTATTACTA ATGAGAGAAA GCGGAAGCTG 
GAACAGCAGT TACAGAGCAT CATGGTTCTC AACCGTCAGT TTGAGCATGT GCAGAACTTC 
ATCAACTGGG CTGTCTGCAG CAAGACCAGT GTCCCTTTCC TGTTCAGCAA AGAGCTGATT 
GTGTTTCAGA TGCAGCGATT GCTGGAGGCA AACTGTAACA CAGATCCGGG CTCCCCCTGG 
AGTATCAGAT TCACCTGGGA GCCTAACTTC TGGACCAAGC AGCTGGCTTC CCTTGGCTGT 
ATAACTACTG AAGGTGGACA GCTGTCCCGG GCAGATACTC CTGCTTATGG AGGCTTACAG 
GGACCATCAT CCTTTTATCA AAGCCACCAG TCCCCAGTGG CTCAGCAAGA GACTCTTAGC 
CACCCCTCAC ATAAGTTCCA GTCTCCAGCA CTGTGTTCTT CATCTGTATG CTGCTCCCAC 
TGCTCCCCAG CCTCGCCTTC CCTCAAAGGC CAGGTCCCCC CACCCAGCAT ACACTCAGCC 
CACAGCTTTA GGCAGCCCTC AGAGATGGCA CCCCAACAGC TAGGCTCTCT GCAGTGCTCT 
ACTCTGCTGC CCAGGGAGAA GGAGCTGCCT TGCAATCCTC AGCCACCAAA ACTGATGCAG 
CCCTGGCTGG AAACCCAGCC CCCCGTGGAG CAGGAGAACA CATCCCAGCG GCTGGGGCAA 
CAGCCAACTT CCCAGCCAGT ATGCATTGTG CCCCCGCAGG ATGTTCAGCA AGGAGCCCAT 
GCCCAACCCA CCTTACAGAC ACCCTCTATC CAAGTCCAGT TTGGCCACCA CCAGAAGCTG 
AAGCTCAGCC ACTTTCAGCA GCAGCCACAG CAGCAACTGC CACCTCCGCC ACCTCTGCCA 
CCCCCACCCC AGCAGCAGCC ACTGCCACCT CTACCTCCAC CCCAGCATCC AGCTTCTAGT 
CAGCATGGGA GCCCTCCTGG GCCTGCCTGT TCCCAGAACA TGGATATAAT GCACCACAAA 
TTTGAGCTGG AGGAGATGCA GAAGGACCTG GAGCTTCTTC TTCAGGCTCA ACAGCCCAGC 
CTACAGCTGA ATCAGACCAA ATCTCCTCAG CATCTTCAGC AAACCATTGT GGGACAGATC 
AACTACATTG TGAGGCAGCC AGCACCCGTC CAGTCCCAGA GCCAAGAGGA TGCTGCACAG 
GCTTCGGATG AGCACCCCAC ATCTGAAGGT CCAAAGCCGC CTCTCCCACT TGACAAGAAT 
ACTGCTGCTA ACTTGCCCCA GGCGTCTGGG GAGGAAACCC CTCCTGGTGT CCCCCCACCA 
GACGGCACCA TCCCGCACTC CTCTCCAAGT GTGGTGAGAA AGCACTCCAC TTCAGTGAGC 
ATCATGGGCT TCTCCAACAC GCTAGAGATG GAGCTGTCAT CTACCAGGCT AGCCAGGACC 
CTGGAGCCGC AGATCCAGAG CGTGAGCAGC CTGACTGCTG CCCCTTCCCA CGTGGTCCCA 
AGCCTGCTGA GCGGCACCCC CACAACTGTG TCCAGCCTGC TGAGCGGTCA GAGCCAGGCT 
GTGCCCAGCC TGACAGTTAG TCCTCTGCAG ACCATACCCA GTCTTGTACG TGGCACACCC 
CGGACCGTGC CCGGTTTGAT GAGTGATTCC TCCCAGGCCA TCACAAGCCT AGCAAGTGAT 
CACTCACAGG CCAGGCCCAA GCTGATGTCT GGTCCCACCC AGACTCTGCC AAGTCTGGCT 
ACTTGTCCAC TGCAGAGCAT GCCTCCGGCT TCTGACACGC AACCGGAAAC TGCATCCAGT 
GGCAGTCCTG GCTCTGGCGG AGCTGTGGGG AGCCTGTGCC CTGGAGATGG GGCTGACCCC 
TCTCTTGGGA ATGCCCTGTG TAAGATGGAA AGTGAGGATT CTTCCCGCTT CACTGACTCT 
CTGGAACAGG ACCCCACAAC TCCTGGTCTG GAGGTTTCCA AGGATTTGGC TATCCCTTCA 
GAACTGGAGG AGCCAATTAA CCTCTCTGTG AAGAAACCTC CAGTGGTCCC AGCAGTCAGC 
ACAGCTATGG CTCTGCAGCA GTACCAGAAC CCAAAAGAGT ATGAGAATTT TGAAGAAGCC 
CTGGAGCTGA ATGTAAAAGA GAACCAGAGT ATCAGGCGAG AGCCTAAGAT TCCCTATGTG 
CGGCTGGAGC GGCTTAAGAT CTGTGCTGCC TCCTCGGGAG AGATGCCTGT GTTCAAGCTG 
AAGCCCCAGA AGAATGAGCA GGATGGGAGC TTCCTGCTGA TCATCGAGTG TGGCACAGAG 
TCCTCCAGCA TGTCCATTAA GGTCAGCCAG AACAACCTGC CTGATGCCAT CCAGGCCCCA 
AGTCTGGGGG GAAGAAAGGT CACTGTCACT TCTCTGGCTG GACAACAGCC ACCAGAAGGG 
GAGGGTGCAT CTCCTGAAGA ACAAAGGCTC ATCCCTCGGA CCCTTGGAGC CAAGAAGGGG 
CCCCCAGTAC CAATAGAGAA TGAGGACTTC TGTGCTGTGT GCCTCAATGG GGGCGAGCTA 
CTGTGCTGTG ACCGCTGCCC TAAGGTGTTC CACCTCTCCT GCCACCTGCC GGCCTTGCTT 
GGCTTCCCAG GGGGAGATTG GGTGTGTACC TTGTGCCGCA GCCTGACCCA GCCCGAGATG 
GAGTATGACT GTGAGAATGC CCGCTATTAC CAACCTGGGA TGCGGGACAC CCCTGGCCTG 
AGCATCTATG ACCAAAAGAA GTGTGAAAAG TTGGTTCTGT CCTTGTGCTG CAATAGCCTC 
AGCCTGCCCT TCCACGAGCC TGTCAGCCCG CTGGCCCGGC ATTACTACCA GATTATCAAG 
AGGCCCATGG ACCTGTCAAT CATCCGGAGG AAGCTGCAAA AGAAGGACCC AGCTCACTAC 
ACCACCCCAG AGGAGGTGGT GTCAGACGTC CGCCTCATGT TCTGGAACTG TGCTAAATTC 
AATTATCCTG ACTCAGAAGT TGCAGAGGCT GGCCGCTGCC TGGAAGTTTT CTTTGAGGGC 
TGGTTGAAGG AGATCTATCC AGAAAAACAG TTTGCCCAGC CGAGGCAGGA GGACTCAGAC 
TCCGAGGAGG TGTCTAGCGA GAGTGGCTAT TCCACTCCCC AGGGCTTCCC ATGGCCTCCC 
TATGTGCAGG AGGGCATCCA ACCCAAGAGG CGGCGGCGAC ATATGGAGAA TGAAAGAGCA 
AAAAGAATGT CATTCCGCTT GGCCAGCAGC GTCTCCCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122784.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978259.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978259.1
CDS	140..3856
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122784.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122784.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122783.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978258.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978258.1
CDS	739..4455
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122783.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122783.2

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122782.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978257.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978257.1
CDS	690..4406
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122782.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122782.2

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122780.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978255.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122780.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978255.1
CDS	673..4389
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122780.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122780.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122781.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978256.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978256.1
CDS	321..4037
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122781.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122781.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122783.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978258.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122783.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978258.1
CDS	756..4472
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122783.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122783.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122780.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978255.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014122780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978255.1
CDS	656..4372
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122780.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122780.2

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_022407812.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263520.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_022407812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263520.1
CDS	118..3834
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_022407812.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407812.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_014122782.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013978257.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

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CloneID	OCe42227
Clone ID Related Accession (Same CDS sequence)	XM_014122784.1 , XM_014122783.2 , XM_014122782.2 , XM_014122780.1 , XM_014122781.1 , XM_014122783.1 , XM_014122780.2 , XM_022407812.1 , XM_014122782.1
Accession Version	XM_014122782.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013978257.1
CDS	707..4423
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_014122782.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014122782.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCCGA GTGCAAGGAG AAGAGGGCAG CGCATATCCT GTGCACCTAC 
TGCAACCGCT GGCTGTGCAG CTCATGCACA GAGGAGCACC GGCATGGCCC CGCCCCTGGG 
GGCCCACTCT TTCCGCGGGC CCACAAGGGA CCACCAGGAG TGAGCAGTGG TTCTGGGGAC 
TTCGCCTTGT ACTGTCCTCT ACACACACAG GAAGTGCTCA AGCTATTCTG CGAGACCTGT 
GATGTGCTCA CGTGCCATAG CTGCCTCATG GTGGAACACA AAGAACACAG GTGTAGACAT 
CTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTAGAAA GTGTGACTAC ACAGGTGGCT 
CATAAGAAAT CTACTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAGGTG 
AAGCATCAAC ACAGGAAGGT GGAAAACCAG ATCAAGATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA CGGGCTCATA GAGGAGTTGG AGGGTATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAACT TCATCAACTG GGCTGTCTGC AGCAAGACCA GTGTCCCTTT CCTGTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGG CAAACTGTAA CACAGATCCG 
GGCTCCCCCT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTGGCT 
TCCCTTGGCT GTATAACTAC TGAAGGTGGA CAGCTGTCCC GGGCAGATAC TCCTGCTTAT 
GGAGGCTTAC AGGGACCATC ATCCTTTTAT CAAAGCCACC AGTCCCCAGT GGCTCAGCAA 
GAGACTCTTA GCCACCCCTC ACATAAGTTC CAGTCTCCAG CACTGTGTTC TTCATCTGTA 
TGCTGCTCCC ACTGCTCCCC AGCCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACTCAG CCCACAGCTT TAGGCAGCCC TCAGAGATGG CACCCCAACA GCTAGGCTCT 
CTGCAGTGCT CTACTCTGCT GCCCAGGGAG AAGGAGCTGC CTTGCAATCC TCAGCCACCA 
AAACTGATGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAA CACATCCCAG 
CGGCTGGGGC AACAGCCAAC TTCCCAGCCA GTATGCATTG TGCCCCCGCA GGATGTTCAG 
CAAGGAGCCC ATGCCCAACC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG CCACTTTCAG CAGCAGCCAC AGCAGCAACT GCCACCTCCG 
CCACCTCTGC CACCCCCACC CCAGCAGCAG CCACTGCCAC CTCTACCTCC ACCCCAGCAT 
CCAGCTTCTA GTCAGCATGG GAGCCCTCCT GGGCCTGCCT GTTCCCAGAA CATGGATATA 
ATGCACCACA AATTTGAGCT GGAGGAGATG CAGAAGGACC TGGAGCTTCT TCTTCAGGCT 
CAACAGCCCA GCCTACAGCT GAATCAGACC AAATCTCCTC AGCATCTTCA GCAAACCATT 
GTGGGACAGA TCAACTACAT TGTGAGGCAG CCAGCACCCG TCCAGTCCCA GAGCCAAGAG 
GATGCTGCAC AGGCTTCGGA TGAGCACCCC ACATCTGAAG GTCCAAAGCC GCCTCTCCCA 
CTTGACAAGA ATACTGCTGC TAACTTGCCC CAGGCGTCTG GGGAGGAAAC CCCTCCTGGT 
GTCCCCCCAC CAGACGGCAC CATCCCGCAC TCCTCTCCAA GTGTGGTGAG AAAGCACTCC 
ACTTCAGTGA GCATCATGGG CTTCTCCAAC ACGCTAGAGA TGGAGCTGTC ATCTACCAGG 
CTAGCCAGGA CCCTGGAGCC GCAGATCCAG AGCGTGAGCA GCCTGACTGC TGCCCCTTCC 
CACGTGGTCC CAAGCCTGCT GAGCGGCACC CCCACAACTG TGTCCAGCCT GCTGAGCGGT 
CAGAGCCAGG CTGTGCCCAG CCTGACAGTT AGTCCTCTGC AGACCATACC CAGTCTTGTA 
CGTGGCACAC CCCGGACCGT GCCCGGTTTG ATGAGTGATT CCTCCCAGGC CATCACAAGC 
CTAGCAAGTG ATCACTCACA GGCCAGGCCC AAGCTGATGT CTGGTCCCAC CCAGACTCTG 
CCAAGTCTGG CTACTTGTCC ACTGCAGAGC ATGCCTCCGG CTTCTGACAC GCAACCGGAA 
ACTGCATCCA GTGGCAGTCC TGGCTCTGGC GGAGCTGTGG GGAGCCTGTG CCCTGGAGAT 
GGGGCTGACC CCTCTCTTGG GAATGCCCTG TGTAAGATGG AAAGTGAGGA TTCTTCCCGC 
TTCACTGACT CTCTGGAACA GGACCCCACA ACTCCTGGTC TGGAGGTTTC CAAGGATTTG 
GCTATCCCTT CAGAACTGGA GGAGCCAATT AACCTCTCTG TGAAGAAACC TCCAGTGGTC 
CCAGCAGTCA GCACAGCTAT GGCTCTGCAG CAGTACCAGA ACCCAAAAGA GTATGAGAAT 
TTTGAAGAAG CCCTGGAGCT GAATGTAAAA GAGAACCAGA GTATCAGGCG AGAGCCTAAG 
ATTCCCTATG TGCGGCTGGA GCGGCTTAAG ATCTGTGCTG CCTCCTCGGG AGAGATGCCT 
GTGTTCAAGC TGAAGCCCCA GAAGAATGAG CAGGATGGGA GCTTCCTGCT GATCATCGAG 
TGTGGCACAG AGTCCTCCAG CATGTCCATT AAGGTCAGCC AGAACAACCT GCCTGATGCC 
ATCCAGGCCC CAAGTCTGGG GGGAAGAAAG GTCACTGTCA CTTCTCTGGC TGGACAACAG 
CCACCAGAAG GGGAGGGTGC ATCTCCTGAA GAACAAAGGC TCATCCCTCG GACCCTTGGA 
GCCAAGAAGG GGCCCCCAGT ACCAATAGAG AATGAGGACT TCTGTGCTGT GTGCCTCAAT 
GGGGGCGAGC TACTGTGCTG TGACCGCTGC CCTAAGGTGT TCCACCTCTC CTGCCACCTG 
CCGGCCTTGC TTGGCTTCCC AGGGGGAGAT TGGGTGTGTA CCTTGTGCCG CAGCCTGACC 
CAGCCCGAGA TGGAGTATGA CTGTGAGAAT GCCCGCTATT ACCAACCTGG GATGCGGGAC 
ACCCCTGGCC TGAGCATCTA TGACCAAAAG AAGTGTGAAA AGTTGGTTCT GTCCTTGTGC 
TGCAATAGCC TCAGCCTGCC CTTCCACGAG CCTGTCAGCC CGCTGGCCCG GCATTACTAC 
CAGATTATCA AGAGGCCCAT GGACCTGTCA ATCATCCGGA GGAAGCTGCA AAAGAAGGAC 
CCAGCTCACT ACACCACCCC AGAGGAGGTG GTGTCAGACG TCCGCCTCAT GTTCTGGAAC 
TGTGCTAAAT TCAATTATCC TGACTCAGAA GTTGCAGAGG CTGGCCGCTG CCTGGAAGTT 
TTCTTTGAGG GCTGGTTGAA GGAGATCTAT CCAGAAAAAC AGTTTGCCCA GCCGAGGCAG 
GAGGACTCAG ACTCCGAGGA GGTGTCTAGC GAGAGTGGCT ATTCCACTCC CCAGGGCTTC 
CCATGGCCTC CCTATGTGCA GGAGGGCATC CAACCCAAGA GGCGGCGGCG ACATATGGAG 
AATGAAAGAG CAAAAAGAAT GTCATTCCGC TTGGCCAGCA GCGTCTCCCA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_005633636.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633693.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

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CloneID	OCe23168
Clone ID Related Accession (Same CDS sequence)	XM_005633636.2 , XM_005633636.3
Accession Version	XM_005633636.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT 
CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC 
CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG 
TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG 
AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC 
TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC 
ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA 
CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT 
GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT 
AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC 
CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG 
TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT 
GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG 
TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA 
GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG 
AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG 
GCCAGCAGCG TCTCCCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633693.1
CDS	39..4202
Translation	MSRREMISGSNLARQGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_005633636.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633636.2

ORF Insert Sequence:

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT 
CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC 
CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG 
TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG 
AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC 
TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC 
ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA 
CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT 
GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT 
AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC 
CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG 
TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT 
GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG 
TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA 
GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG 
AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG 
GCCAGCAGCG TCTCCCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_005633636.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633693.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

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CloneID	OCe23168
Clone ID Related Accession (Same CDS sequence)	XM_005633636.2 , XM_005633636.3
Accession Version	XM_005633636.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT 
CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC 
CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG 
TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG 
AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC 
TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC 
ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA 
CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT 
GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT 
AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC 
CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG 
TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT 
GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG 
TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA 
GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG 
AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG 
GCCAGCAGCG TCTCCCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633693.1
CDS	94..4257
Translation	MSRREMISGSNLARQGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKMESEDSSRFTDSLEQDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_005633636.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633636.3

ORF Insert Sequence:

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGATGGAAA GTGAGGATTC TTCCCGCTTC ACTGACTCTC TGGAACAGGA CCCCACAACT 
CCTGGTCTGG AGGTTTCCAA GGATTTGGCT ATCCCTTCAG AACTGGAGGA GCCAATTAAC 
CTCTCTGTGA AGAAACCTCC AGTGGTCCCA GCAGTCAGCA CAGCTATGGC TCTGCAGCAG 
TACCAGAACC CAAAAGAGTA TGAGAATTTT GAAGAAGCCC TGGAGCTGAA TGTAAAAGAG 
AACCAGAGTA TCAGGCGAGA GCCTAAGATT CCCTATGTGC GGCTGGAGCG GCTTAAGATC 
TGTGCTGCCT CCTCGGGAGA GATGCCTGTG TTCAAGCTGA AGCCCCAGAA GAATGAGCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACAGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGA ACAACCTGCC TGATGCCATC CAGGCCCCAA GTCTGGGGGG AAGAAAGGTC 
ACTGTCACTT CTCTGGCTGG ACAACAGCCA CCAGAAGGGG AGGGTGCATC TCCTGAAGAA 
CAAAGGCTCA TCCCTCGGAC CCTTGGAGCC AAGAAGGGGC CCCCAGTACC AATAGAGAAT 
GAGGACTTCT GTGCTGTGTG CCTCAATGGG GGCGAGCTAC TGTGCTGTGA CCGCTGCCCT 
AAGGTGTTCC ACCTCTCCTG CCACCTGCCG GCCTTGCTTG GCTTCCCAGG GGGAGATTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTATGACTG TGAGAATGCC 
CGCTATTACC AACCTGGGAT GCGGGACACC CCTGGCCTGA GCATCTATGA CCAAAAGAAG 
TGTGAAAAGT TGGTTCTGTC CTTGTGCTGC AATAGCCTCA GCCTGCCCTT CCACGAGCCT 
GTCAGCCCGC TGGCCCGGCA TTACTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTACA CCACCCCAGA GGAGGTGGTG 
TCAGACGTCC GCCTCATGTT CTGGAACTGT GCTAAATTCA ATTATCCTGA CTCAGAAGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTTTTC TTTGAGGGCT GGTTGAAGGA GATCTATCCA 
GAAAAACAGT TTGCCCAGCC GAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGCGAG 
AGTGGCTATT CCACTCCCCA GGGCTTCCCA TGGCCTCCCT ATGTGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGGCGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTCCGCTTG 
GCCAGCAGCG TCTCCCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_005633637.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633694.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe23169
Clone ID Related Accession (Same CDS sequence)	XM_005633637.2 , XM_005633637.3
Accession Version	XM_005633637.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tripartite motif-containing protein 66 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG 
GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT 
ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG 
CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG 
GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC 
CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG 
GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT 
GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA 
GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC 
TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC 
CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT 
GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC 
TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG 
CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC 
CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT 
CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633694.1
CDS	39..4157
Translation	MSRREMISGSNLARQGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_005633637.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633637.2

ORF Insert Sequence:

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG 
GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT 
ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG 
CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG 
GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC 
CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG 
GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT 
GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA 
GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC 
TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC 
CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT 
GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC 
TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG 
CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC 
CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT 
CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_005633637.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633694.1 Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

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CloneID	OCe23169
Clone ID Related Accession (Same CDS sequence)	XM_005633637.2 , XM_005633637.3
Accession Version	XM_005633637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tripartite motif-containing protein 66 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG 
GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT 
ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG 
CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG 
GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC 
CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG 
GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT 
GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA 
GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC 
TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC 
CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT 
GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC 
TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG 
CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC 
CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT 
CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633694.1
CDS	94..4212
Translation	MSRREMISGSNLARQGVELARSTRCCTLQDIMGRAPVPVTSTATDTGLRFMGLPLVDQKHRNGQMEAMILTCSLCQQDLQNVGARFLPCQHLLCKNCFQGFIQELRQVSKAHRTVPPELISCPGCKRVYLTRDVNEHFFLQCFSTGRSKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHGPAPGGPLFPRAHKGPPGVSSGSGDFALYCPLHTQEVLKLFCETCDVLTCHSCLMVEHKEHRCRHLEEVLQNQRMLLESVTTQVAHKKSTLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLEANCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQLSRADTPAYGGLQGPSSFYQSHQSPVAQQETLSHPSHKFQSPALCSSSVCCSHCSPASPSLKGQVPPPSIHSAHSFRQPSEMAPQQLGSLQCSTLLPREKELPCNPQPPKLMQPWLETQPPVEQENTSQRLGQQPTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPLPPPPQQQPLPPLPPPQHPASSQHGSPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLNQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEDAAQASDEHPTSEGPKPPLPLDKNTAANLPQASGEETPPGVPPPDGTIPHSSPSVVRKHSTSVSIMGFSNTLEMELSSTRLARTLEPQIQSVSSLTAAPSHVVPSLLSGTPTTVSSLLSGQSQAVPSLTVSPLQTIPSLVRGTPRTVPGLMSDSSQAITSLASDHSQARPKLMSGPTQTLPSLATCPLQSMPPASDTQPETASSGSPGSGGAVGSLCPGDGADPSLGNALCKDPTTPGLEVSKDLAIPSELEEPINLSVKKPPVVPAVSTAMALQQYQNPKEYENFEEALELNVKENQSIRREPKIPYVRLERLKICAASSGEMPVFKLKPQKNEQDGSFLLIIECGTESSSMSIKVSQNNLPDAIQAPSLGGRKVTVTSLAGQQPPEGEGASPEEQRLIPRTLGAKKGPPVPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHLPALLGFPGGDWVCTLCRSLTQPEMEYDCENARYYQPGMRDTPGLSIYDQKKCEKLVLSLCCNSLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKQFAQPRQEDSDSEEVSSESGYSTPQGFPWPPYVQEGIQPKRRRRHMENERAKRMSFRLASSVSQV

Target ORF information:

RefSeq Version	XM_005633637.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633637.3

ORF Insert Sequence:

ATGAGCAGAA GGGAGATGAT ATCAGGCAGC AATCTGGCAA GACAGGGAGT GGAGCTTGCT 
CGATCTACAC GCTGCTGCAC ACTTCAGGAT ATCATGGGAA GAGCCCCAGT CCCGGTCACA 
AGCACAGCCA CGGATACGGG CTTGAGATTT ATGGGGCTGC CACTAGTTGA CCAGAAACAC 
AGGAATGGTC AGATGGAGGC CATGATATTG ACCTGCTCAT TGTGCCAACA AGACCTGCAA 
AATGTGGGCG CCCGGTTCCT ACCCTGCCAG CATTTGCTCT GCAAGAACTG CTTCCAGGGC 
TTCATCCAGG AGCTGAGACA GGTTTCCAAA GCTCACAGGA CCGTTCCACC TGAGCTCATC 
TCCTGTCCTG GGTGTAAGCG AGTATATCTT ACCAGGGATG TAAATGAACA TTTTTTTCTG 
CAGTGCTTTT CTACAGGGCG ATCCAAGATG GCCAGGAACT GCTCCGAGTG CAAGGAGAAG 
AGGGCAGCGC ATATCCTGTG CACCTACTGC AACCGCTGGC TGTGCAGCTC ATGCACAGAG 
GAGCACCGGC ATGGCCCCGC CCCTGGGGGC CCACTCTTTC CGCGGGCCCA CAAGGGACCA 
CCAGGAGTGA GCAGTGGTTC TGGGGACTTC GCCTTGTACT GTCCTCTACA CACACAGGAA 
GTGCTCAAGC TATTCTGCGA GACCTGTGAT GTGCTCACGT GCCATAGCTG CCTCATGGTG 
GAACACAAAG AACACAGGTG TAGACATCTT GAAGAAGTTT TGCAAAACCA GAGGATGCTT 
CTAGAAAGTG TGACTACACA GGTGGCTCAT AAGAAATCTA CTCTACAGAC ATCTGCAAAG 
CAAATTGAGG ACAGGATTTT TGAGGTGAAG CATCAACACA GGAAGGTGGA AAACCAGATC 
AAGATGGCCA AGATGGTTCT GATGAATGAG CTGAACAAAC AGGCCAACGG GCTCATAGAG 
GAGTTGGAGG GTATTACTAA TGAGAGAAAG CGGAAGCTGG AACAGCAGTT ACAGAGCATC 
ATGGTTCTCA ACCGTCAGTT TGAGCATGTG CAGAACTTCA TCAACTGGGC TGTCTGCAGC 
AAGACCAGTG TCCCTTTCCT GTTCAGCAAA GAGCTGATTG TGTTTCAGAT GCAGCGATTG 
CTGGAGGCAA ACTGTAACAC AGATCCGGGC TCCCCCTGGA GTATCAGATT CACCTGGGAG 
CCTAACTTCT GGACCAAGCA GCTGGCTTCC CTTGGCTGTA TAACTACTGA AGGTGGACAG 
CTGTCCCGGG CAGATACTCC TGCTTATGGA GGCTTACAGG GACCATCATC CTTTTATCAA 
AGCCACCAGT CCCCAGTGGC TCAGCAAGAG ACTCTTAGCC ACCCCTCACA TAAGTTCCAG 
TCTCCAGCAC TGTGTTCTTC ATCTGTATGC TGCTCCCACT GCTCCCCAGC CTCGCCTTCC 
CTCAAAGGCC AGGTCCCCCC ACCCAGCATA CACTCAGCCC ACAGCTTTAG GCAGCCCTCA 
GAGATGGCAC CCCAACAGCT AGGCTCTCTG CAGTGCTCTA CTCTGCTGCC CAGGGAGAAG 
GAGCTGCCTT GCAATCCTCA GCCACCAAAA CTGATGCAGC CCTGGCTGGA AACCCAGCCC 
CCCGTGGAGC AGGAGAACAC ATCCCAGCGG CTGGGGCAAC AGCCAACTTC CCAGCCAGTA 
TGCATTGTGC CCCCGCAGGA TGTTCAGCAA GGAGCCCATG CCCAACCCAC CTTACAGACA 
CCCTCTATCC AAGTCCAGTT TGGCCACCAC CAGAAGCTGA AGCTCAGCCA CTTTCAGCAG 
CAGCCACAGC AGCAACTGCC ACCTCCGCCA CCTCTGCCAC CCCCACCCCA GCAGCAGCCA 
CTGCCACCTC TACCTCCACC CCAGCATCCA GCTTCTAGTC AGCATGGGAG CCCTCCTGGG 
CCTGCCTGTT CCCAGAACAT GGATATAATG CACCACAAAT TTGAGCTGGA GGAGATGCAG 
AAGGACCTGG AGCTTCTTCT TCAGGCTCAA CAGCCCAGCC TACAGCTGAA TCAGACCAAA 
TCTCCTCAGC ATCTTCAGCA AACCATTGTG GGACAGATCA ACTACATTGT GAGGCAGCCA 
GCACCCGTCC AGTCCCAGAG CCAAGAGGAT GCTGCACAGG CTTCGGATGA GCACCCCACA 
TCTGAAGGTC CAAAGCCGCC TCTCCCACTT GACAAGAATA CTGCTGCTAA CTTGCCCCAG 
GCGTCTGGGG AGGAAACCCC TCCTGGTGTC CCCCCACCAG ACGGCACCAT CCCGCACTCC 
TCTCCAAGTG TGGTGAGAAA GCACTCCACT TCAGTGAGCA TCATGGGCTT CTCCAACACG 
CTAGAGATGG AGCTGTCATC TACCAGGCTA GCCAGGACCC TGGAGCCGCA GATCCAGAGC 
GTGAGCAGCC TGACTGCTGC CCCTTCCCAC GTGGTCCCAA GCCTGCTGAG CGGCACCCCC 
ACAACTGTGT CCAGCCTGCT GAGCGGTCAG AGCCAGGCTG TGCCCAGCCT GACAGTTAGT 
CCTCTGCAGA CCATACCCAG TCTTGTACGT GGCACACCCC GGACCGTGCC CGGTTTGATG 
AGTGATTCCT CCCAGGCCAT CACAAGCCTA GCAAGTGATC ACTCACAGGC CAGGCCCAAG 
CTGATGTCTG GTCCCACCCA GACTCTGCCA AGTCTGGCTA CTTGTCCACT GCAGAGCATG 
CCTCCGGCTT CTGACACGCA ACCGGAAACT GCATCCAGTG GCAGTCCTGG CTCTGGCGGA 
GCTGTGGGGA GCCTGTGCCC TGGAGATGGG GCTGACCCCT CTCTTGGGAA TGCCCTGTGT 
AAGGACCCCA CAACTCCTGG TCTGGAGGTT TCCAAGGATT TGGCTATCCC TTCAGAACTG 
GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCAGTGG TCCCAGCAGT CAGCACAGCT 
ATGGCTCTGC AGCAGTACCA GAACCCAAAA GAGTATGAGA ATTTTGAAGA AGCCCTGGAG 
CTGAATGTAA AAGAGAACCA GAGTATCAGG CGAGAGCCTA AGATTCCCTA TGTGCGGCTG 
GAGCGGCTTA AGATCTGTGC TGCCTCCTCG GGAGAGATGC CTGTGTTCAA GCTGAAGCCC 
CAGAAGAATG AGCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC AGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGAACAAC CTGCCTGATG CCATCCAGGC CCCAAGTCTG 
GGGGGAAGAA AGGTCACTGT CACTTCTCTG GCTGGACAAC AGCCACCAGA AGGGGAGGGT 
GCATCTCCTG AAGAACAAAG GCTCATCCCT CGGACCCTTG GAGCCAAGAA GGGGCCCCCA 
GTACCAATAG AGAATGAGGA CTTCTGTGCT GTGTGCCTCA ATGGGGGCGA GCTACTGTGC 
TGTGACCGCT GCCCTAAGGT GTTCCACCTC TCCTGCCACC TGCCGGCCTT GCTTGGCTTC 
CCAGGGGGAG ATTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAT 
GACTGTGAGA ATGCCCGCTA TTACCAACCT GGGATGCGGG ACACCCCTGG CCTGAGCATC 
TATGACCAAA AGAAGTGTGA AAAGTTGGTT CTGTCCTTGT GCTGCAATAG CCTCAGCCTG 
CCCTTCCACG AGCCTGTCAG CCCGCTGGCC CGGCATTACT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTACACCACC 
CCAGAGGAGG TGGTGTCAGA CGTCCGCCTC ATGTTCTGGA ACTGTGCTAA ATTCAATTAT 
CCTGACTCAG AAGTTGCAGA GGCTGGCCGC TGCCTGGAAG TTTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ATCCAGAAAA ACAGTTTGCC CAGCCGAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GCGAGAGTGG CTATTCCACT CCCCAGGGCT TCCCATGGCC TCCCTATGTG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGG CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTCC GCTTGGCCAG CAGCGTCTCC CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRIM66
Entrez Gene ID	485379
Full Name	tripartite motif containing 66
General protein information	Preferred Names tripartite motif containing 66 Names tripartite motif-containing protein 66
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Canis lupus familiaris (dog)	TRIM66	XP_005633693.1
Bos taurus (cattle)	TRIM66	XP_002693153.3
Mus musculus (house mouse)	Trim66	NP_001164383.1
Gallus gallus (chicken)	TRIM66	XP_004941449.1
Xenopus tropicalis (tropical clawed frog)	trim66	XP_002942909.2
Homo sapiens (human)	TRIM66	NP_055633.1
Rattus norvegicus (Norway rat)	Trim66	NP_001178768.1
	TRIM66	XP_001097566.2
Pan troglodytes (chimpanzee)	TRIM66	XP_003313014.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRIM66 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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