LOC100485706 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100485706
Entrez Gene ID	100485706
Full Name	toll-like receptor22
Synonyms	tlr22
General protein information	Preferred Names toll-like receptor22 Names toll-like receptor 13
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002942535.4	XP_002942581.1	toll-like receptor 13
XM_002942535.5	XP_002942581.2	toll-like receptor 13

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Toll-6	NP_001246765.1
Xenopus tropicalis (tropical clawed frog)	LOC100485706	XP_002942581.1
Anopheles gambiae (African malaria mosquito)	TOLL6	XP_320172.2

Gene Ontology

The following LOC100485706 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100485706 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa08209	XM_002942535.4 Latest version!	Xenopus tropicalis toll-like receptor22 (LOC100485706), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa54513	XM_002942535.5 Latest version!	Xenopus tropicalis toll-like receptor22 (LOC100485706), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100485706 ( XM_002942535.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942581.1 Xenopus tropicalis toll-like receptor22 (LOC100485706), mRNA.

Price & Availability↑

CloneID	OXa08209
Clone ID Related Accession (Same CDS sequence)	XM_002942535.4
Accession Version	XM_002942535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	toll-like receptor 13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942535.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGGC TGATGCTGCA GTTTAAGATG TTTTCATTAT TTAATTGCCT TGTGCTGTTT 
CATTTGGTTA CAAGAGGGGT CTCGTTTGCA ATTAAAGGCT GTGAAGTGCA TAGGCAGTAC 
AATCTGAAGG TTCTCTGCTA CAACAGGGAT TTAACCAAGG TCCCAGCACA GCTTCCAGAG 
TTAAGCTTCA TGCTCGACCT TTCCCATAAC ATTATCAAGA CCATAAACAG GACAGACTTC 
CAGAATCTTA CAGTCCTTCA AAATCTAAAC CTTTCAAACA ATCACATCCA ATATATGGAG 
AATGGAGCCT TTGGGAAACT TGTTGCTCTG CAACTTCTCA ACCTTACCAA AAACAGGATT 
GGCTCTCTGT CCAGCCGCAC GTTTGAAGGA CTGCAAAACC TAAGCACACT GTTACTGGAT 
AACAACAATA TTGTTACCTT AGAGCCCAAT ACGTTTCTAC ATTTGTACAA CCTTAAAACT 
ATCAGTCTTT CCTACAACTG TTTTTACAAA CTGAAGACTC TGAACCCTGC CTTTCAGATC 
GGGACCCTCA GAGAGATAAA CATAGCCAAC TGTAGCATAC AAACATTTTC CACTGAGGAC 
ATAGTAAATG TTTCTGCTTC TCTTCGGTCA GTCGATGCCT CCAGAAATCC ATTTTCAGTT 
ATCAGTTTCC CAATGAGTAT CTTAGAGAAC CTTACTAACC TGGACATATC TCTCACTCAG 
AGGCCCATTT TATGGTACGT TAAAGAACCT TGCTTTTTGA GGGGACTGAA GAAGCTAAAT 
ATTAAAGAGA ATAATATAAA ACCACCGGCT ATAATAGGTA TAATTAAAAA TCTGTCATGT 
AGTTCCCTTG AAGAACTTAA CCTAAGTTAC TTAAACCTAA CGGACTCTGA CCAACTAATC 
CAAGAACTAT GCCTTAGACA CCCGAAAGTT CAAACACTCA AACTTACTGG TAACCAGTAC 
ACAGAGTTCA GAGAAGATAC ATTTCAAAAC TGTACTTCTG TAAAAGTGTT AGATTTATCT 
CACAACAGGC TGCATTCTGC GCCTGGGTCT ACTTTCCACC ATCTTGCCTC TATCAGAGAG 
CTGTCTCTGG CCAATAACTC ACTTACTGCT ATACCTGATA ATTTCTCCTT TATAACCTCC 
TTGCAGAAGC TGAACCTCAG TTACAACCAT GTTAAGGAGG TGAATTTAAA CGACTCTTTC 
AGCAATTTAA AGGAACTTGA CTTATCTGGA AACAAAATGA CGTTTTTTCT TTCCTCGGGA 
CTAGGCAACT GGAACCTACA AGATTTGAAT CTGAGAGAAA ATTACCTTTT GGATATCAGC 
GCTTCTTTTG CATCCAGTCT GCCCACGCTG CAGAGACTCA CCATTAGCAA GAATAAACTA 
AGCTCTCTGT CAACCAATAC TTTTCAAAAT GTAACCTTCC TGAAACATCT GAATCTTGCA 
GACAATCAGA TAGAGGTGAT AGATCCTGGT GCCTTTTCTG GTCTTGACAA GCTGCGCACC 
TTGATAATTG GTAGCAACAA GATAACAAGG AACACTCTCC ACAATATGAC TTTCCAAGGC 
TTGGGGTCAC TCCTTGAGCT ACAGCTTTTT GGCAACTATC TTAATTACGA TTCCTCAAAT 
TATCTTGATA TTGTGCCATT CCTTCCATTA AAATCTTTAA AGATGATCAC TCTAAACAGT 
CAAGGTCACA ATGGAATGCA GAATTTACCT ATCAACTTCT TTGAAGGTCT GCTGGCATTG 
GAAAGGATTC ATGCAGGCAA TTTAGCCCTG TCTAGCATTG ACTCAAGAAC ATTTTCATAT 
ACTCCACAAC TGAGAGAACT TGATATCAGC GAAAATCCAA TCAAGATTCT TGACCCATTT 
TTGTTTAGAA ATGTACCACA TCTCACAGAG CTGCACATCA GTAAAACTCG CATGGAATCG 
CTGGATTTTC TCATTCTATC AAACTTGACT CACCTGAAGC TTCTCCGCGC AGTAGATAGC 
CAGCTGAACT CATTCACTTC ACAACAGGGT GCGGCTTTAC CATCTCTGAG ATTCTTGGAC 
CTGCGCAACA ACCCACTGAC CTGCATCTGT GACAACCAGT GGTTTATAGA TTGGGTTCAC 
AATGATACCA AGACCCAAGT CCTTCATTTT TATGAGTACA CGTGTGCTTA CCCACCTGTC 
GCAAGGAACT CCAAACTGTC TACTTTCAAC CTTTCTTCCT GTAGGTTAGA CTATTCCTTT 
ATTTTCTTTC TTACATCAAC CCTGTTCATA ACTACTTTTA TCATTTCTGC AACTGTGTGG 
AATTTTTGGA GATGGCATGT GATTTATGCT TACTACATTA CCCTGGCTTT CCTCTATAAC 
AGAAGGTGGC AAGGGAAGAA ACAAAAATGT AAGTATGATG CTTTCATATC ATACAACTTC 
CATGATGAGA AATGGGTCTT CAGGCAACTT GTTCCTAATC TAGAAGGGAG ATATAACTGG 
AGGCTTTGTC TTCATCACAG AGACTTTGAA CCAGGAAAAG CTATAGTTGA TAACATAGTT 
GATAGCATCT ATAGCAGCAG GAAAACCATC TGTATTATCA GCAGGCACTA TTTAGAGAGT 
GAATGGTGTT CCAAAGAAAT CCAGTTGGCC AGTTTCCGCC TCTTTGAGGA ACATACAGAT 
GTTCTGATTC TGTTGTTCCT GGAACAAATT CCCGATCACC ACTTATCCCC CTACCACCAG 
ATAAGGAAAG TTATAAAAAA GAAGACATAC TTAATATGGC CACAGGAGAG AAATGCTACC 
AGCATCTTCT GGTATAGGGT GAGTCAGGCA TTGCAGACTG AAGAAAGAGA AGAGGATGAA 
AAATGTCTGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942581.1
CDS	481..3315
Translation	MGWLMLQFKMFSLFNCLVLFHLVTRGVSFAIKGCEVHRQYNLKVLCYNRDLTKVPAQLPELSFMLDLSHNIIKTINRTDFQNLTVLQNLNLSNNHIQYMENGAFGKLVALQLLNLTKNRIGSLSSRTFEGLQNLSTLLLDNNNIVTLEPNTFLHLYNLKTISLSYNCFYKLKTLNPAFQIGTLREINIANCSIQTFSTEDIVNVSASLRSVDASRNPFSVISFPMSILENLTNLDISLTQRPILWYVKEPCFLRGLKKLNIKENNIKPPAIIGIIKNLSCSSLEELNLSYLNLTDSDQLIQELCLRHPKVQTLKLTGNQYTEFREDTFQNCTSVKVLDLSHNRLHSAPGSTFHHLASIRELSLANNSLTAIPDNFSFITSLQKLNLSYNHVKEVNLNDSFSNLKELDLSGNKMTFFLSSGLGNWNLQDLNLRENYLLDISASFASSLPTLQRLTISKNKLSSLSTNTFQNVTFLKHLNLADNQIEVIDPGAFSGLDKLRTLIIGSNKITRNTLHNMTFQGLGSLLELQLFGNYLNYDSSNYLDIVPFLPLKSLKMITLNSQGHNGMQNLPINFFEGLLALERIHAGNLALSSIDSRTFSYTPQLRELDISENPIKILDPFLFRNVPHLTELHISKTRMESLDFLILSNLTHLKLLRAVDSQLNSFTSQQGAALPSLRFLDLRNNPLTCICDNQWFIDWVHNDTKTQVLHFYEYTCAYPPVARNSKLSTFNLSSCRLDYSFIFFLTSTLFITTFIISATVWNFWRWHVIYAYYITLAFLYNRRWQGKKQKCKYDAFISYNFHDEKWVFRQLVPNLEGRYNWRLCLHHRDFEPGKAIVDNIVDSIYSSRKTICIISRHYLESEWCSKEIQLASFRLFEEHTDVLILLFLEQIPDHHLSPYHQIRKVIKKKTYLIWPQERNATSIFWYRVSQALQTEEREEDEKCLL

Target ORF information:

RefSeq Version	XM_002942535.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis toll-like receptor22 (LOC100485706), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942535.4

ORF Insert Sequence:

ATGGGTTGGC TGATGCTGCA GTTTAAGATG TTTTCATTAT TTAATTGCCT TGTGCTGTTT 
CATTTGGTTA CAAGAGGGGT CTCGTTTGCA ATTAAAGGCT GTGAAGTGCA TAGGCAGTAC 
AATCTGAAGG TTCTCTGCTA CAACAGGGAT TTAACCAAGG TCCCAGCACA GCTTCCAGAG 
TTAAGCTTCA TGCTCGACCT TTCCCATAAC ATTATCAAGA CCATAAACAG GACAGACTTC 
CAGAATCTTA CAGTCCTTCA AAATCTAAAC CTTTCAAACA ATCACATCCA ATATATGGAG 
AATGGAGCCT TTGGGAAACT TGTTGCTCTG CAACTTCTCA ACCTTACCAA AAACAGGATT 
GGCTCTCTGT CCAGCCGCAC GTTTGAAGGA CTGCAAAACC TAAGCACACT GTTACTGGAT 
AACAACAATA TTGTTACCTT AGAGCCCAAT ACGTTTCTAC ATTTGTACAA CCTTAAAACT 
ATCAGTCTTT CCTACAACTG TTTTTACAAA CTGAAGACTC TGAACCCTGC CTTTCAGATC 
GGGACCCTCA GAGAGATAAA CATAGCCAAC TGTAGCATAC AAACATTTTC CACTGAGGAC 
ATAGTAAATG TTTCTGCTTC TCTTCGGTCA GTCGATGCCT CCAGAAATCC ATTTTCAGTT 
ATCAGTTTCC CAATGAGTAT CTTAGAGAAC CTTACTAACC TGGACATATC TCTCACTCAG 
AGGCCCATTT TATGGTACGT TAAAGAACCT TGCTTTTTGA GGGGACTGAA GAAGCTAAAT 
ATTAAAGAGA ATAATATAAA ACCACCGGCT ATAATAGGTA TAATTAAAAA TCTGTCATGT 
AGTTCCCTTG AAGAACTTAA CCTAAGTTAC TTAAACCTAA CGGACTCTGA CCAACTAATC 
CAAGAACTAT GCCTTAGACA CCCGAAAGTT CAAACACTCA AACTTACTGG TAACCAGTAC 
ACAGAGTTCA GAGAAGATAC ATTTCAAAAC TGTACTTCTG TAAAAGTGTT AGATTTATCT 
CACAACAGGC TGCATTCTGC GCCTGGGTCT ACTTTCCACC ATCTTGCCTC TATCAGAGAG 
CTGTCTCTGG CCAATAACTC ACTTACTGCT ATACCTGATA ATTTCTCCTT TATAACCTCC 
TTGCAGAAGC TGAACCTCAG TTACAACCAT GTTAAGGAGG TGAATTTAAA CGACTCTTTC 
AGCAATTTAA AGGAACTTGA CTTATCTGGA AACAAAATGA CGTTTTTTCT TTCCTCGGGA 
CTAGGCAACT GGAACCTACA AGATTTGAAT CTGAGAGAAA ATTACCTTTT GGATATCAGC 
GCTTCTTTTG CATCCAGTCT GCCCACGCTG CAGAGACTCA CCATTAGCAA GAATAAACTA 
AGCTCTCTGT CAACCAATAC TTTTCAAAAT GTAACCTTCC TGAAACATCT GAATCTTGCA 
GACAATCAGA TAGAGGTGAT AGATCCTGGT GCCTTTTCTG GTCTTGACAA GCTGCGCACC 
TTGATAATTG GTAGCAACAA GATAACAAGG AACACTCTCC ACAATATGAC TTTCCAAGGC 
TTGGGGTCAC TCCTTGAGCT ACAGCTTTTT GGCAACTATC TTAATTACGA TTCCTCAAAT 
TATCTTGATA TTGTGCCATT CCTTCCATTA AAATCTTTAA AGATGATCAC TCTAAACAGT 
CAAGGTCACA ATGGAATGCA GAATTTACCT ATCAACTTCT TTGAAGGTCT GCTGGCATTG 
GAAAGGATTC ATGCAGGCAA TTTAGCCCTG TCTAGCATTG ACTCAAGAAC ATTTTCATAT 
ACTCCACAAC TGAGAGAACT TGATATCAGC GAAAATCCAA TCAAGATTCT TGACCCATTT 
TTGTTTAGAA ATGTACCACA TCTCACAGAG CTGCACATCA GTAAAACTCG CATGGAATCG 
CTGGATTTTC TCATTCTATC AAACTTGACT CACCTGAAGC TTCTCCGCGC AGTAGATAGC 
CAGCTGAACT CATTCACTTC ACAACAGGGT GCGGCTTTAC CATCTCTGAG ATTCTTGGAC 
CTGCGCAACA ACCCACTGAC CTGCATCTGT GACAACCAGT GGTTTATAGA TTGGGTTCAC 
AATGATACCA AGACCCAAGT CCTTCATTTT TATGAGTACA CGTGTGCTTA CCCACCTGTC 
GCAAGGAACT CCAAACTGTC TACTTTCAAC CTTTCTTCCT GTAGGTTAGA CTATTCCTTT 
ATTTTCTTTC TTACATCAAC CCTGTTCATA ACTACTTTTA TCATTTCTGC AACTGTGTGG 
AATTTTTGGA GATGGCATGT GATTTATGCT TACTACATTA CCCTGGCTTT CCTCTATAAC 
AGAAGGTGGC AAGGGAAGAA ACAAAAATGT AAGTATGATG CTTTCATATC ATACAACTTC 
CATGATGAGA AATGGGTCTT CAGGCAACTT GTTCCTAATC TAGAAGGGAG ATATAACTGG 
AGGCTTTGTC TTCATCACAG AGACTTTGAA CCAGGAAAAG CTATAGTTGA TAACATAGTT 
GATAGCATCT ATAGCAGCAG GAAAACCATC TGTATTATCA GCAGGCACTA TTTAGAGAGT 
GAATGGTGTT CCAAAGAAAT CCAGTTGGCC AGTTTCCGCC TCTTTGAGGA ACATACAGAT 
GTTCTGATTC TGTTGTTCCT GGAACAAATT CCCGATCACC ACTTATCCCC CTACCACCAG 
ATAAGGAAAG TTATAAAAAA GAAGACATAC TTAATATGGC CACAGGAGAG AAATGCTACC 
AGCATCTTCT GGTATAGGGT GAGTCAGGCA TTGCAGACTG AAGAAAGAGA AGAGGATGAA 
AAATGTCTGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100485706 ( XM_002942535.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942581.2 Xenopus tropicalis toll-like receptor22 (LOC100485706), mRNA.

Price & Availability↑

CloneID	OXa54513
Clone ID Related Accession (Same CDS sequence)	XM_002942535.5
Accession Version	XM_002942535.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	toll-like receptor 13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942535.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGGC TGATGCTGCA GTTTAAGATG TTTTCATTAT TTAATTGCCT TGTGCTGTTT 
CATTTGGTTA CAAGAGGGGT CTCGTTTGCA ATTAAAGGCT GTGAAGTGCA TAGGCAGTAC 
AATCTGAAGG TTCTCTGCTA CAACAGGGAT TTAACCAAGG TCCCAGCACA GCTTCCAGAG 
TTAAGCTTCA TGCTCGACCT TTCCCATAAC ATTATCAAGA CCATAAACAG GACAGACTTC 
CAGAATCTTA CAGTCCTTCA AAATCTAAAC CTTTCAAACA ATCGCATCCA ATATATGGAG 
AATGGAGCCT TTGGGAAACT TGTTGCTCTG CAACTTCTCA ACCTTACCAA AAACAGGATT 
GGCTCTCTGT CCAGCCGCAC GTTTGAAGGA CTGCAAAACC TAAGCACACT GTTACTGGAT 
AACAACAATA TTGTTACCTT AGAGCCCAAT ACGTTTCTAC ATTTGTACAA CCTTAAAACT 
ATCAGTCTTT CCTACAACTG TTTTTACAAA CTGAAGACTC TGAACCCTGC CTTTCAGATC 
GGGACCCTCA GAGAGATAAA CATAGCAAAC TGTAGCATAC AAACATTTTC CACTGAGGAC 
ATAGTAAATG TTTCTGCTTC TCTTCGGTCA GTCGATGCCT CCAGAAATCC ATTTTCAGTT 
ATCAGTTTCC CAATGAGTAT CTTAGAGAAC CTTACTAACC TAGACATATC TCTCACTCAG 
AGGCCCATTT TATGGTACGT TAAAGAACCT TGCTTTTTGA GGGGACTGAA GAAGCTAAAT 
ATTAAAGAGA ATAATATAAA ACCACCGGCT ATAATAGGTA TAATTAAAAA TCTGTCATGT 
AGCTCCCTTG AAGAACTTAA CCTAAGTTAC TTAAACCTAA CGGACTCTGA CCAACTAATC 
CAAGAACTAT GCCTTAGACA CCCGAAAGTT CAAACACTCA AACTTACTGG TAACCAGTAC 
ACAGAGTTCA GAGAAGATAC ATTTCAAAAC TGTACTTCTG TAAAAGTGTT AGATTTATCT 
CACAACAGGC TGCATTCTGC GCCTGGGTCT ACTTTCCACC ATCTTGCCTC TATCAGAGAG 
CTGTCTCTGG CCAATAACTC ACTTACTGCT ATACCTGATA ATTTCTCCTT TATAACCTCC 
TTGCAGAAGC TGAACCTCAG TTACAACCAT GTTAAGGAGG TGAATCTAAA CAACTCTTTC 
AGCAATTTAA AGGAACTTGA CTTATCTGGA AACAAAATGA CGTTTTTTCT TTCCTCGGGA 
CTAGGCAACT GGAACCTACA AGATTTGAAT CTGAGAGAAA ATTACCTTTT GGATATCAGC 
GCTTCTTTTG CATCCAGTCT GCCCACGCTG CAGAGACTCA CCATTAGCAA GAATAAGCTA 
AGCTCTCTGT CAACCAATAC TTTTCAAAAT GTAACCTTCC TGAAACATCT GAATCTTGCA 
GACAATCAGA TAGAGGTGAT AGATCCTGGT GCCTTTTCTG GTCTTGACAA GCTGCGCACC 
TTGATAATTG GTAGCAACAA GATAACAAGG AACACTCTCC ACAATATGAC TTTCCAAGGC 
TTGGGGTCAC TCCTTGAGCT ACAGCTTTTT GGCAACTATC TTAATTACGA TTCCTCAAAT 
TATCTTGATA TTGTGCCATT CCTTCCATTA AAATCTTTAA AGATGATCAC TCTAAACAGT 
CAAGGTCACA ATGGAATGCA GAATTTACCT ATCAACTTCT TTGAAGGTCT GCTGGCATTG 
GAAAGGATTC ATGCAGGCAA TTTAGCCCTG TCTAGCATTG ACTCAAGAAC ATTTTCATAT 
ACTCCACAAC TGAGAGAACT TGATATCAGC GAAAATCCAA TCAAGATTCT TGACCCATTT 
TTGTTTAGAA ATGTACCACA TCTCACAGAG CTGCACATCA GTAAAACTCG CATGGAATCG 
CTGGATTTTC TCATTCTATC AAACTTGACT CACCTGAAGC TTCTCCGCGC AGTAGATAGC 
CAGCTGAACT CATTCACTTC ACAACAGGGT GCGGCTTTAC CATCTCTGAG ATTCTTGGAC 
CTGCGCAACA ACCCACTGAC CTGCATCTGT GACAACCAGT GGTTTATAGA TTGGGTTCAC 
AATGATACCA AGACCCAAGT CCTTCATTTT TATGAGTACA CGTGTGCTTA CCCACCTGTC 
GCAAGGAACT CCAAACTGTC TACTTTCAAC CTTTCTTCCT GTAGGTTAGA CTATTCCTTT 
ATTTTCTTTC TTACATCAAC CCTGTTCATA ACTACTTTTA TCATTTCTGC AACTGTGTGG 
AATTTTTGGA GATGGCATGT GATTTATGCT TACTACATTA CCCTGGCTTT CCTCTATAAC 
AGAAGGTGGC AAGGGAAGAA ACAAAAATGT AAGTATGATG CTTTCATATC ATACAACTTC 
CATGATGAGA AATGGGTCTT CAGGCAACTT GTTCCTAATC TAGAAGGGAG ATATAACTGG 
AGGCTTTGTC TTCATCACAG AGACTTTGAA CCAGGAAAAG CTATAGTTGA TAACATAGTT 
GATAGCATCT ATAGCAGCAG GAAAACCATC TGTATTATCA GCAGGCACTA TTTAGAGAGT 
GAATGGTGTT CCAAAGAAAT CCAGTTGGCC AGTTTCCGCC TCTTTGAGGA ACATACAGAT 
GTTCTGATTC TGTTGTTCCT GGAACAAATT CCCGATCACC ACTTATCCCC CTACCACCAG 
ATAAGGAAAG TTATAAAAAA GAAGACATAC TTAATATGGC CACAGGAGAG AAATGCTACC 
AGCATCTTCT GGTATAGGGT GAGTCAGGCA TTGCAGACTG AAGAAAGAGA AGAGGATGAA 
AAATGTCTGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942581.2
CDS	607..3441
Translation	MGWLMLQFKMFSLFNCLVLFHLVTRGVSFAIKGCEVHRQYNLKVLCYNRDLTKVPAQLPELSFMLDLSHNIIKTINRTDFQNLTVLQNLNLSNNRIQYMENGAFGKLVALQLLNLTKNRIGSLSSRTFEGLQNLSTLLLDNNNIVTLEPNTFLHLYNLKTISLSYNCFYKLKTLNPAFQIGTLREINIANCSIQTFSTEDIVNVSASLRSVDASRNPFSVISFPMSILENLTNLDISLTQRPILWYVKEPCFLRGLKKLNIKENNIKPPAIIGIIKNLSCSSLEELNLSYLNLTDSDQLIQELCLRHPKVQTLKLTGNQYTEFREDTFQNCTSVKVLDLSHNRLHSAPGSTFHHLASIRELSLANNSLTAIPDNFSFITSLQKLNLSYNHVKEVNLNNSFSNLKELDLSGNKMTFFLSSGLGNWNLQDLNLRENYLLDISASFASSLPTLQRLTISKNKLSSLSTNTFQNVTFLKHLNLADNQIEVIDPGAFSGLDKLRTLIIGSNKITRNTLHNMTFQGLGSLLELQLFGNYLNYDSSNYLDIVPFLPLKSLKMITLNSQGHNGMQNLPINFFEGLLALERIHAGNLALSSIDSRTFSYTPQLRELDISENPIKILDPFLFRNVPHLTELHISKTRMESLDFLILSNLTHLKLLRAVDSQLNSFTSQQGAALPSLRFLDLRNNPLTCICDNQWFIDWVHNDTKTQVLHFYEYTCAYPPVARNSKLSTFNLSSCRLDYSFIFFLTSTLFITTFIISATVWNFWRWHVIYAYYITLAFLYNRRWQGKKQKCKYDAFISYNFHDEKWVFRQLVPNLEGRYNWRLCLHHRDFEPGKAIVDNIVDSIYSSRKTICIISRHYLESEWCSKEIQLASFRLFEEHTDVLILLFLEQIPDHHLSPYHQIRKVIKKKTYLIWPQERNATSIFWYRVSQALQTEEREEDEKCLL

Target ORF information:

RefSeq Version	XM_002942535.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis toll-like receptor22 (LOC100485706), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942535.5

ORF Insert Sequence:

ATGGGTTGGC TGATGCTGCA GTTTAAGATG TTTTCATTAT TTAATTGCCT TGTGCTGTTT 
CATTTGGTTA CAAGAGGGGT CTCGTTTGCA ATTAAAGGCT GTGAAGTGCA TAGGCAGTAC 
AATCTGAAGG TTCTCTGCTA CAACAGGGAT TTAACCAAGG TCCCAGCACA GCTTCCAGAG 
TTAAGCTTCA TGCTCGACCT TTCCCATAAC ATTATCAAGA CCATAAACAG GACAGACTTC 
CAGAATCTTA CAGTCCTTCA AAATCTAAAC CTTTCAAACA ATCGCATCCA ATATATGGAG 
AATGGAGCCT TTGGGAAACT TGTTGCTCTG CAACTTCTCA ACCTTACCAA AAACAGGATT 
GGCTCTCTGT CCAGCCGCAC GTTTGAAGGA CTGCAAAACC TAAGCACACT GTTACTGGAT 
AACAACAATA TTGTTACCTT AGAGCCCAAT ACGTTTCTAC ATTTGTACAA CCTTAAAACT 
ATCAGTCTTT CCTACAACTG TTTTTACAAA CTGAAGACTC TGAACCCTGC CTTTCAGATC 
GGGACCCTCA GAGAGATAAA CATAGCAAAC TGTAGCATAC AAACATTTTC CACTGAGGAC 
ATAGTAAATG TTTCTGCTTC TCTTCGGTCA GTCGATGCCT CCAGAAATCC ATTTTCAGTT 
ATCAGTTTCC CAATGAGTAT CTTAGAGAAC CTTACTAACC TAGACATATC TCTCACTCAG 
AGGCCCATTT TATGGTACGT TAAAGAACCT TGCTTTTTGA GGGGACTGAA GAAGCTAAAT 
ATTAAAGAGA ATAATATAAA ACCACCGGCT ATAATAGGTA TAATTAAAAA TCTGTCATGT 
AGCTCCCTTG AAGAACTTAA CCTAAGTTAC TTAAACCTAA CGGACTCTGA CCAACTAATC 
CAAGAACTAT GCCTTAGACA CCCGAAAGTT CAAACACTCA AACTTACTGG TAACCAGTAC 
ACAGAGTTCA GAGAAGATAC ATTTCAAAAC TGTACTTCTG TAAAAGTGTT AGATTTATCT 
CACAACAGGC TGCATTCTGC GCCTGGGTCT ACTTTCCACC ATCTTGCCTC TATCAGAGAG 
CTGTCTCTGG CCAATAACTC ACTTACTGCT ATACCTGATA ATTTCTCCTT TATAACCTCC 
TTGCAGAAGC TGAACCTCAG TTACAACCAT GTTAAGGAGG TGAATCTAAA CAACTCTTTC 
AGCAATTTAA AGGAACTTGA CTTATCTGGA AACAAAATGA CGTTTTTTCT TTCCTCGGGA 
CTAGGCAACT GGAACCTACA AGATTTGAAT CTGAGAGAAA ATTACCTTTT GGATATCAGC 
GCTTCTTTTG CATCCAGTCT GCCCACGCTG CAGAGACTCA CCATTAGCAA GAATAAGCTA 
AGCTCTCTGT CAACCAATAC TTTTCAAAAT GTAACCTTCC TGAAACATCT GAATCTTGCA 
GACAATCAGA TAGAGGTGAT AGATCCTGGT GCCTTTTCTG GTCTTGACAA GCTGCGCACC 
TTGATAATTG GTAGCAACAA GATAACAAGG AACACTCTCC ACAATATGAC TTTCCAAGGC 
TTGGGGTCAC TCCTTGAGCT ACAGCTTTTT GGCAACTATC TTAATTACGA TTCCTCAAAT 
TATCTTGATA TTGTGCCATT CCTTCCATTA AAATCTTTAA AGATGATCAC TCTAAACAGT 
CAAGGTCACA ATGGAATGCA GAATTTACCT ATCAACTTCT TTGAAGGTCT GCTGGCATTG 
GAAAGGATTC ATGCAGGCAA TTTAGCCCTG TCTAGCATTG ACTCAAGAAC ATTTTCATAT 
ACTCCACAAC TGAGAGAACT TGATATCAGC GAAAATCCAA TCAAGATTCT TGACCCATTT 
TTGTTTAGAA ATGTACCACA TCTCACAGAG CTGCACATCA GTAAAACTCG CATGGAATCG 
CTGGATTTTC TCATTCTATC AAACTTGACT CACCTGAAGC TTCTCCGCGC AGTAGATAGC 
CAGCTGAACT CATTCACTTC ACAACAGGGT GCGGCTTTAC CATCTCTGAG ATTCTTGGAC 
CTGCGCAACA ACCCACTGAC CTGCATCTGT GACAACCAGT GGTTTATAGA TTGGGTTCAC 
AATGATACCA AGACCCAAGT CCTTCATTTT TATGAGTACA CGTGTGCTTA CCCACCTGTC 
GCAAGGAACT CCAAACTGTC TACTTTCAAC CTTTCTTCCT GTAGGTTAGA CTATTCCTTT 
ATTTTCTTTC TTACATCAAC CCTGTTCATA ACTACTTTTA TCATTTCTGC AACTGTGTGG 
AATTTTTGGA GATGGCATGT GATTTATGCT TACTACATTA CCCTGGCTTT CCTCTATAAC 
AGAAGGTGGC AAGGGAAGAA ACAAAAATGT AAGTATGATG CTTTCATATC ATACAACTTC 
CATGATGAGA AATGGGTCTT CAGGCAACTT GTTCCTAATC TAGAAGGGAG ATATAACTGG 
AGGCTTTGTC TTCATCACAG AGACTTTGAA CCAGGAAAAG CTATAGTTGA TAACATAGTT 
GATAGCATCT ATAGCAGCAG GAAAACCATC TGTATTATCA GCAGGCACTA TTTAGAGAGT 
GAATGGTGTT CCAAAGAAAT CCAGTTGGCC AGTTTCCGCC TCTTTGAGGA ACATACAGAT 
GTTCTGATTC TGTTGTTCCT GGAACAAATT CCCGATCACC ACTTATCCCC CTACCACCAG 
ATAAGGAAAG TTATAAAAAA GAAGACATAC TTAATATGGC CACAGGAGAG AAATGCTACC 
AGCATCTTCT GGTATAGGGT GAGTCAGGCA TTGCAGACTG AAGAAAGAGA AGAGGATGAA 
AAATGTCTGC TGTAG