ttll5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttll5
Entrez Gene ID	100158545
Full Name	tubulin tyrosine ligase like 5
General protein information	Preferred Names tubulin tyrosine ligase like 5 Names tubulin polyglutamylase TTLL5 tubulin tyrosine ligase-like family, member 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096165.1	XP_017951654.1	tubulin polyglutamylase TTLL5 isoform X4
XM_012968058.2	XP_012823512.1	tubulin polyglutamylase TTLL5 isoform X1
NM_001127979.1	NP_001121451.1	tubulin polyglutamylase TTLL5
XM_012968058.3	XP_012823512.2	tubulin polyglutamylase TTLL5 isoform X1
XM_012968059.3	XP_012823513.2	tubulin polyglutamylase TTLL5 isoform X2
XM_012968059.2	XP_012823513.1	tubulin polyglutamylase TTLL5 isoform X2
XM_012968060.2	XP_012823514.1	tubulin polyglutamylase TTLL5 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TTLL5	XP_005623734.1
Rattus norvegicus (Norway rat)	Ttll5	XP_006240435.1
Pan troglodytes (chimpanzee)	TTLL5	XP_001161309.3
	LOC703116	XP_002805202.1
Mus musculus (house mouse)	Ttll5	NP_001074892.2
Bos taurus (cattle)	TTLL5	XP_005212294.1
Gallus gallus (chicken)	TTLL5	NP_001026375.1
Homo sapiens (human)	TTLL5	NP_055887.3
Danio rerio (zebrafish)	LOC100538301	XP_003200448.2
Xenopus tropicalis (tropical clawed frog)	ttll5	NP_001121451.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ttll5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttll5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45978	XM_018096165.1 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa63513	XM_012968058.3 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63514	XM_012968059.3 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa24322	NM_001127979.1 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa32165	XM_012968058.2 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa32166	XM_012968059.2 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa32167	XM_012968060.2 Latest version!	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttll5 ( XM_018096165.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951654.1 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa45978
Clone ID Related Accession (Same CDS sequence)	XM_018096165.1
Accession Version	XM_018096165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGTAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGCC CCCAGAACAG ACTTACATGT 
GAGAGAAGCG TAGTTATGAG CTCAGCACCT AGCCAGCCAT CAGTCAGTAA ACCAGGATAT 
TCTCAGGGGT GTAATGTGTC TGACGGTCCC ATCATAATCA CTAACTCAGC AGTCAATGGG 
TTACCTGAAA GTTGCTCCCA AAGAAACAGT AGCACTCATG TGCTTAGTAA TGATGCAAGT 
TTATGTTCTA CTATTGTTGG AAGCAATGGA ATCCACGTGT CCCCTGCAAA ACTTACTGTT 
ACCCCTGGTT CATGGGCCAA ATCTGGTTCT CGCCCTCACT CCAGCAGCCT TGGAACATTT 
TCCTCCTTTC AAAGTGCAGC TCAGATCTAC AGCCAAAAAC TGAGACGCCC AAGCTCCACC 
CGTTCAGCAA CCATCAGAGT GCTTTACGCT CTCGCTGTAG CTCAGCCGGA TCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951654.1
CDS	142..2898
Translation	MPEMVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPHIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTHQRPLSANDIDTGLQVGNREKAVRRTSCLLGLSIEELKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADLSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGPQNRLTCERSVVMSSAPSQPSVSKPGYSQGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSATIRVLYALAVAQPDQ

Target ORF information:

RefSeq Version	XM_018096165.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096165.1

ORF Insert Sequence:

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGTAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGCC CCCAGAACAG ACTTACATGT 
GAGAGAAGCG TAGTTATGAG CTCAGCACCT AGCCAGCCAT CAGTCAGTAA ACCAGGATAT 
TCTCAGGGGT GTAATGTGTC TGACGGTCCC ATCATAATCA CTAACTCAGC AGTCAATGGG 
TTACCTGAAA GTTGCTCCCA AAGAAACAGT AGCACTCATG TGCTTAGTAA TGATGCAAGT 
TTATGTTCTA CTATTGTTGG AAGCAATGGA ATCCACGTGT CCCCTGCAAA ACTTACTGTT 
ACCCCTGGTT CATGGGCCAA ATCTGGTTCT CGCCCTCACT CCAGCAGCCT TGGAACATTT 
TCCTCCTTTC AAAGTGCAGC TCAGATCTAC AGCCAAAAAC TGAGACGCCC AAGCTCCACC 
CGTTCAGCAA CCATCAGAGT GCTTTACGCT CTCGCTGTAG CTCAGCCGGA TCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttll5 ( XM_012968058.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823512.2 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63513
Clone ID Related Accession (Same CDS sequence)	XM_012968058.3
Accession Version	XM_012968058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGGAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGCC CCCAGAACAG ACTTACATGT 
GAGAGAAGCG TAGTTATGAG CTCAGCACCT AGCCAGCCAT CAGTCAGTAA ACCAGGATAT 
TCTCAGGGGT GTAATGTGTC TGACGGTCCC ATCATAATCA CTAACTCAGC AGTCAATGGG 
TTACCTGAAA GTTGCTCCCA AAGAAACAGT AGCACTCATG TGCTTAGTAA TGATGCAAGT 
TTATGTTCTA CTATTGTTGG AAGCAATGGA ATCCACGTGT CCCCTGCAAA ACTTACTGTT 
ACCCCTGGTT CATGGGCCAA ATCTGGTTCT CGCCCTCACT CCAGCAGCCT TGGAACATTT 
TCCTCCTTTC AAAGTGCAGC TCAGATCTAC AGCCAAAAAC TGAGACGCCC AAGCTCCACC 
CGTTCAGCCA GCAACCATCA GAGTGCTTTA CGCTCTCGCT GTAGCTCAGC CGGATCAGTA 
AAAGAGCTGG ATGTTCCATA CAATGAAAGT GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA 
GAGAGACAAT CAAGTCAACA GTACTCCAAA CAACTTAGAC TTCTAACACA GCAGTTAACC 
AGCATGAACA TAAAAGATGG TGCTTTTGGA AGTGGAAGTT TTCGTCATTG TTCTGCAAAG 
AGCTTCTGTG GAAGAGCTGT TCATGCAGGC ACTGAAACTG TGGAATCAAT AACCAGAGAT 
ATACAAAGGA GACGTTCTGC ATGGGAAAGT GATCAAGAAA GTGGCACTTT CAGCTTTTCG 
TCAGATGTCC CTTTACAGCA TCAACCCGAT CAAATGTATA GCGCAAAAGG AGGACAACAT 
CCCGACTCTG CTATTATCTC TCTGCCCAAC CAAACCTGCA CCCTTCTGCC CACCCCACCA 
GTCAGTCACA AGCAGTCTGC AGCCAGAACA TTATCCGCAA CACGATTAGT GAGGGTAGCA 
CCAGTGGAGC AACATGGGAC CCCCACATCT ACAATTGTCA GTGACTTTGG AACCCCATCA 
CAAGGTTCCA TGGAGGCCAC TCAAATTATA TTTGCCAGAG CTAGACCTTC AGCTCCCAAA 
ATCGACATTA AAGGGCAAAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823512.2
CDS	142..3528
Translation	MPEMVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPHIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTHQRPLSANDIDTGLQGGNREKAVRRTSCLLGLSIEELKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADLSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGPQNRLTCERSVVMSSAPSQPSVSKPGYSQGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSASNHQSALRSRCSSAGSVKELDVPYNESAVAASLQRLAERQSSQQYSKQLRLLTQQLTSMNIKDGAFGSGSFRHCSAKSFCGRAVHAGTETVESITRDIQRRRSAWESDQESGTFSFSSDVPLQHQPDQMYSAKGGQHPDSAIISLPNQTCTLLPTPPVSHKQSAARTLSATRLVRVAPVEQHGTPTSTIVSDFGTPSQGSMEATQIIFARARPSAPKIDIKGQRK

Target ORF information:

RefSeq Version	XM_012968058.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968058.3

ORF Insert Sequence:

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGGAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGCC CCCAGAACAG ACTTACATGT 
GAGAGAAGCG TAGTTATGAG CTCAGCACCT AGCCAGCCAT CAGTCAGTAA ACCAGGATAT 
TCTCAGGGGT GTAATGTGTC TGACGGTCCC ATCATAATCA CTAACTCAGC AGTCAATGGG 
TTACCTGAAA GTTGCTCCCA AAGAAACAGT AGCACTCATG TGCTTAGTAA TGATGCAAGT 
TTATGTTCTA CTATTGTTGG AAGCAATGGA ATCCACGTGT CCCCTGCAAA ACTTACTGTT 
ACCCCTGGTT CATGGGCCAA ATCTGGTTCT CGCCCTCACT CCAGCAGCCT TGGAACATTT 
TCCTCCTTTC AAAGTGCAGC TCAGATCTAC AGCCAAAAAC TGAGACGCCC AAGCTCCACC 
CGTTCAGCCA GCAACCATCA GAGTGCTTTA CGCTCTCGCT GTAGCTCAGC CGGATCAGTA 
AAAGAGCTGG ATGTTCCATA CAATGAAAGT GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA 
GAGAGACAAT CAAGTCAACA GTACTCCAAA CAACTTAGAC TTCTAACACA GCAGTTAACC 
AGCATGAACA TAAAAGATGG TGCTTTTGGA AGTGGAAGTT TTCGTCATTG TTCTGCAAAG 
AGCTTCTGTG GAAGAGCTGT TCATGCAGGC ACTGAAACTG TGGAATCAAT AACCAGAGAT 
ATACAAAGGA GACGTTCTGC ATGGGAAAGT GATCAAGAAA GTGGCACTTT CAGCTTTTCG 
TCAGATGTCC CTTTACAGCA TCAACCCGAT CAAATGTATA GCGCAAAAGG AGGACAACAT 
CCCGACTCTG CTATTATCTC TCTGCCCAAC CAAACCTGCA CCCTTCTGCC CACCCCACCA 
GTCAGTCACA AGCAGTCTGC AGCCAGAACA TTATCCGCAA CACGATTAGT GAGGGTAGCA 
CCAGTGGAGC AACATGGGAC CCCCACATCT ACAATTGTCA GTGACTTTGG AACCCCATCA 
CAAGGTTCCA TGGAGGCCAC TCAAATTATA TTTGCCAGAG CTAGACCTTC AGCTCCCAAA 
ATCGACATTA AAGGGCAAAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttll5 ( XM_012968059.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823513.2 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X2, mRNA.

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CloneID	OXa63514
Clone ID Related Accession (Same CDS sequence)	XM_012968059.3
Accession Version	XM_012968059.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGGAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGGT GTAATGTGTC TGACGGTCCC 
ATCATAATCA CTAACTCAGC AGTCAATGGG TTACCTGAAA GTTGCTCCCA AAGAAACAGT 
AGCACTCATG TGCTTAGTAA TGATGCAAGT TTATGTTCTA CTATTGTTGG AAGCAATGGA 
ATCCACGTGT CCCCTGCAAA ACTTACTGTT ACCCCTGGTT CATGGGCCAA ATCTGGTTCT 
CGCCCTCACT CCAGCAGCCT TGGAACATTT TCCTCCTTTC AAAGTGCAGC TCAGATCTAC 
AGCCAAAAAC TGAGACGCCC AAGCTCCACC CGTTCAGCCA GCAACCATCA GAGTGCTTTA 
CGCTCTCGCT GTAGCTCAGC CGGATCAGTA AAAGAGCTGG ATGTTCCATA CAATGAAAGT 
GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA GAGAGACAAT CAAGTCAACA GTACTCCAAA 
CAACTTAGAC TTCTAACACA GCAGTTAACC AGCATGAACA TAAAAGATGG TGCTTTTGGA 
AGTGGAAGTT TTCGTCATTG TTCTGCAAAG AGCTTCTGTG GAAGAGCTGT TCATGCAGGC 
ACTGAAACTG TGGAATCAAT AACCAGAGAT ATACAAAGGA GACGTTCTGC ATGGGAAAGT 
GATCAAGAAA GTGGCACTTT CAGCTTTTCG TCAGATGTCC CTTTACAGCA TCAACCCGAT 
CAAATGCAGT ATAGCGCAAA AGGAGGACAA CATCCCGACT CTGCTATTAT CTCTCTGCCC 
AACCAAACCT GCACCCTTCT GCCCACCCCA CCAGTCAGTC ACAAGCAGTC TGCAGCCAGA 
ACATTATCCG CAACACGATT AGTGAGGGTA GCACCAGTGG AGCAACATGG GACCCCCACA 
TCTACAATTG TCAGTGACTT TGGAACCCCA TCACAAGGTT CCATGGAGGC CACTCAAATT 
ATATTTGCCA GAGCTAGACC TTCAGCTCCC AAAATCGACA TTAAAGGGCA AAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823513.2
CDS	142..3441
Translation	MPEMVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPHIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTHQRPLSANDIDTGLQGGNREKAVRRTSCLLGLSIEELKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADLSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSASNHQSALRSRCSSAGSVKELDVPYNESAVAASLQRLAERQSSQQYSKQLRLLTQQLTSMNIKDGAFGSGSFRHCSAKSFCGRAVHAGTETVESITRDIQRRRSAWESDQESGTFSFSSDVPLQHQPDQMQYSAKGGQHPDSAIISLPNQTCTLLPTPPVSHKQSAARTLSATRLVRVAPVEQHGTPTSTIVSDFGTPSQGSMEATQIIFARARPSAPKIDIKGQRK

Target ORF information:

RefSeq Version	XM_012968059.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968059.3

ORF Insert Sequence:

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGGAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGGT GTAATGTGTC TGACGGTCCC 
ATCATAATCA CTAACTCAGC AGTCAATGGG TTACCTGAAA GTTGCTCCCA AAGAAACAGT 
AGCACTCATG TGCTTAGTAA TGATGCAAGT TTATGTTCTA CTATTGTTGG AAGCAATGGA 
ATCCACGTGT CCCCTGCAAA ACTTACTGTT ACCCCTGGTT CATGGGCCAA ATCTGGTTCT 
CGCCCTCACT CCAGCAGCCT TGGAACATTT TCCTCCTTTC AAAGTGCAGC TCAGATCTAC 
AGCCAAAAAC TGAGACGCCC AAGCTCCACC CGTTCAGCCA GCAACCATCA GAGTGCTTTA 
CGCTCTCGCT GTAGCTCAGC CGGATCAGTA AAAGAGCTGG ATGTTCCATA CAATGAAAGT 
GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA GAGAGACAAT CAAGTCAACA GTACTCCAAA 
CAACTTAGAC TTCTAACACA GCAGTTAACC AGCATGAACA TAAAAGATGG TGCTTTTGGA 
AGTGGAAGTT TTCGTCATTG TTCTGCAAAG AGCTTCTGTG GAAGAGCTGT TCATGCAGGC 
ACTGAAACTG TGGAATCAAT AACCAGAGAT ATACAAAGGA GACGTTCTGC ATGGGAAAGT 
GATCAAGAAA GTGGCACTTT CAGCTTTTCG TCAGATGTCC CTTTACAGCA TCAACCCGAT 
CAAATGCAGT ATAGCGCAAA AGGAGGACAA CATCCCGACT CTGCTATTAT CTCTCTGCCC 
AACCAAACCT GCACCCTTCT GCCCACCCCA CCAGTCAGTC ACAAGCAGTC TGCAGCCAGA 
ACATTATCCG CAACACGATT AGTGAGGGTA GCACCAGTGG AGCAACATGG GACCCCCACA 
TCTACAATTG TCAGTGACTT TGGAACCCCA TCACAAGGTT CCATGGAGGC CACTCAAATT 
ATATTTGCCA GAGCTAGACC TTCAGCTCCC AAAATCGACA TTAAAGGGCA AAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttll5 ( NM_001127979.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001121451.1 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), mRNA.

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CloneID	OXa24322
Clone ID Related Accession (Same CDS sequence)	NM_001127979.1
Accession Version	NM_001127979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166210.1. ##Evidence-Data-START## Transcript exon combination :: BC166210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCCCC GTGGACAGCA GGACGAACAG AGCGAGGAAG ATGATGATTC CAAAAAGGGT 
GAATACTCTT GCATTCTGTG GGCTGGTGGC TCTCGAAAGG TTCCTATTGT AATGTTTCAT 
GCAGAAGCCG TTCTTCACAA AAACCTGTCA CTGCGTGCAG TAGGAGAGCG CTATAAATTA 
TCCTATAAGA TTGTTCGCAC AGACAGCCGC TTAGTACGCA GCATTCTTTC TGCCCATGGA 
TTTCAGGAGG TAAATGCCAA CAGCAATGAC TTCAACATTA TGTGGACTGG GTCCCACGTA 
AAGCCTTACA TCATGAGGAG CCTTACCAAC TTTCAAAAAG TCAATCACTT TCCCAGATCC 
TATGAGCTGA CCCGTAAGGA CCGACTGTAC AAAAATGTGC AGCGCATGCA GCAAAGTCAT 
GGCTTTAAAA ACTTCCACCT TTTGCCCCAG ACATATCTGC TACCTGCTGA ATATCAGGAC 
TTTTGCACTG CTTTTGCCAA AGATCGTGGT CCTTGGATAG TGAAGCCAGT TGCCTCATCA 
CGTGGCCGTG GTGTTTACTT GATTAATTCT CCATCTCTTA TCAGTATGGA GGATAACATC 
CTGGTGTCTC GGTATATTGG CAACCCCCTT CTTATTGATG GATTTAAATT TGATGTTCGT 
CTGTATGTGC TAATCACTTC ATATGACCCT TTAGTCATTT ACCTATATGA AGAAGGACTT 
ACCAGGTTTG CCACAGCAAA GTATGACCGG GCTGCCAAAA ATATAAAAAA TCAGTTTATG 
CACCTGACTA ACTACAGTGT AAACAAGAAA AGCGGGGACT ATGTAAGTTG TGATGACCCA 
GATGTAGAAG ATTATGGGAA CAAATGGAGC ATGAGTGCCA TGCTGCGATA TTTAAAACAG 
GATGGGAAAG ATACAGCGGC TCTCATGTCA CAGGTGGAAG ATCTAATCAT TAAAACAATA 
GTTTCCGCCG AGCTTCCTAT TGCATCCGCA TGTAAATCCT TAATTACCCA CAGGGGAAAC 
TGCTTTGAAC TCTATGGGTT TGATGTGCTG ATTGATGGCA ACTTGAAGCC CTGGTTGTTG 
GAAGTGAACC TGTCTCCATC ATTAGCTTGT GATGCCCCTT TGGACTTAAA GGTGAAAGCC 
AGTATGATAT CAGACATGTT TACCCTAGTT GGTGTTGAGT GTCAGGATCC ACAACAGAGG 
TTTGGACGTG CAAGTAGTTC TTTGTATGAC AAACGAACAC AGAAATCCAC ACACCAGCGT 
CCGCTCTCTG CTAATGACAT AGACACTGGT TTGCAGGTAG GCAACAGAGA AAAGGCTGTG 
AGGCGGACAA GCTGCTTACT AGGGCTTTCC ATAGAGGAGT TGAAGATATT GCGCCGAGTT 
CAAGATGAGT ATGAGAGAAG AGGAGGGTTT GTTCGCATTT TTCCCAGGCA TAACACATGG 
CAACTTTATG GGTCTTTCCT GGAATATAAA ACCTCTCTGA ATTACATGTT AGTAACTCAT 
CTCTTCCCAA ACAGGGCTGC TGGGAATGAC CACTGTGAAA AGAATTGGGA CCCCAGGATG 
CATGCAGCTT TTTATGAACG GAAACTTGTA TCATTGCATC TTCGCAGGGC GCGGCACCGT 
GGGCTGACAC GCAAGACGGG CTTATCACAT GCTCCCCAGT GCTCTGATCA TGAACAGAGT 
AGCAAAGAGC AAGAAGAGCA GGAGGAAGAG GAGGAACTGG ATGAAAACCA CGAATTAGAG 
GTGGTGCAGG AAAAAGTTTC TTCTCCAGAC TCCTCCAAAA TAATCATACC ACCACCTCGC 
ATCAGCCTCA TGGATATTCT ACGGAAAGGT GCAGACTTCA GTAAAGTCCA GGCAAGAAAT 
GCATTCTCCT GCTACCTGCA GCGGGTGCAG AATCGTCTTC AAACTGAGCG AAACCCAGAG 
AGAGTACAAC CAAAGGAGGA GGAGCAAATA GAATTAGTGA TGCGGTTCCT CCAGCGTGGT 
GCTGCAAATT TGAAGCGGTC TCTGCCTCTG AATCTCCCAG GACAATCTGT CCCATACCTT 
GAAAGGCGCC ATTTGCTGGC AAAACTGCTT GGAGATTTTG TTGCTTTGTA TAATCAGGAA 
ACACAACAGA TGCAGAACAG TGAGGAGACA CAGAGCAATG AAGAATGTGG GGTTAATCCT 
GACGATTTTG AGGCATTTGT AGCTGATGCC AGTGAAAATG AATTGGAAGA GGTCCTCACC 
TTCTACACAC AAAAGAATAA ATCTGCAAGT GTTTTTCTTG GGACCCCAAA CAATGCAGAC 
AGGAGAGAAA CTGGGAAACC ACCAGGAGGC CCCCAGAACA GACTTACATG TGAGAGAAGC 
GTAGTTATGA GCTCAGCACC TAGCCAGCCA TCAGTCAGTA AACCAGGATA TTCTCAGGGG 
TGTAATGTGT CTGACGGTCC CATCATAATC ACTAACTCAG CAGTCAATGG GTTACCTGAA 
AGTTGCTCCC AAAGAAACAG TAGCACTCAT GTGCTTAGTA ATGATGCAAG TTTATGTTCT 
ACTATTGTTG GAAGCAATGG AATCCACGTG TCCCCTGCAA AACTTACTGT TACCCCTGGT 
TCATGGGCCA AATCTGGTTC TCGCCCTCAC TCCAGCAGCC TTGGAACATT TTCCTCCTTT 
CAAAGTGCAG CTCAGATCTA CAGCCAAAAA CTGAGACGCC CAAGCTCCAC CCGTTCAGCC 
AGCAACCATC AGAGTGCTTT ACGCTCTCGC TGTAGCTCAG CCGGATCAGT AAAAGAGCTG 
GATGTTCCAT ACAATGAAAG TGCAGTGGCC GCTTCTCTTC AGCGTCTGGC AGAGAGACAA 
TCAAGTCAAC AGTACTCCAA ACAACTTAGA CTTCTAACAC AGCAGTTAAC CAGCATGAAC 
ATAAAAGATG GTGCTTTTGG AAGTGGAAGT TTTCGTCATT GTTCTGCAAA GAGCTTCTGT 
GGAAGAGCTG TTCATGCAGG CACTGAAACT GTGGAATCAA TAACCAGAGA TATACAAAGG 
AGACGTTCTG CATGGGAAAG TGATCAAGAA AGTGGCACTT TCAGCTTTTC GTCAGATGTC 
CCTTTACAGC ATCAACCCGA TCAAATGCAG TATAGCGCAA AAGGAGGACA ACATCCCGAC 
TCTGCTATTA TCTCTCTGCC CAACCAAACC TGCACCCTTC TGCCCACCCC ACCAGTCAGT 
CACAAGCAGT CTGCAGCCAG AACATTATCC GCAACACGAT TAGTGAGGGT AGCACCAGTG 
GAGCAACATG GGACCCCCAC ATCTACAATT GTCAGTGACT TTGGAACCCC ATCACAAGGT 
TCCATGGAGG CCACTCAAAT TATATTTGCC AGAGCTAGAC CTTCAGCTCC CAAAATCGAC 
ATTAAAGGGC AAAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121451.1
CDS	156..3536
Translation	MVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPYIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTHQRPLSANDIDTGLQVGNREKAVRRTSCLLGLSIEELKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADFSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGPQNRLTCERSVVMSSAPSQPSVSKPGYSQGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSASNHQSALRSRCSSAGSVKELDVPYNESAVAASLQRLAERQSSQQYSKQLRLLTQQLTSMNIKDGAFGSGSFRHCSAKSFCGRAVHAGTETVESITRDIQRRRSAWESDQESGTFSFSSDVPLQHQPDQMQYSAKGGQHPDSAIISLPNQTCTLLPTPPVSHKQSAARTLSATRLVRVAPVEQHGTPTSTIVSDFGTPSQGSMEATQIIFARARPSAPKIDIKGQRK

Target ORF information:

RefSeq Version	NM_001127979.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127979.1

ORF Insert Sequence:

ATGGTGCCCC GTGGACAGCA GGACGAACAG AGCGAGGAAG ATGATGATTC CAAAAAGGGT 
GAATACTCTT GCATTCTGTG GGCTGGTGGC TCTCGAAAGG TTCCTATTGT AATGTTTCAT 
GCAGAAGCCG TTCTTCACAA AAACCTGTCA CTGCGTGCAG TAGGAGAGCG CTATAAATTA 
TCCTATAAGA TTGTTCGCAC AGACAGCCGC TTAGTACGCA GCATTCTTTC TGCCCATGGA 
TTTCAGGAGG TAAATGCCAA CAGCAATGAC TTCAACATTA TGTGGACTGG GTCCCACGTA 
AAGCCTTACA TCATGAGGAG CCTTACCAAC TTTCAAAAAG TCAATCACTT TCCCAGATCC 
TATGAGCTGA CCCGTAAGGA CCGACTGTAC AAAAATGTGC AGCGCATGCA GCAAAGTCAT 
GGCTTTAAAA ACTTCCACCT TTTGCCCCAG ACATATCTGC TACCTGCTGA ATATCAGGAC 
TTTTGCACTG CTTTTGCCAA AGATCGTGGT CCTTGGATAG TGAAGCCAGT TGCCTCATCA 
CGTGGCCGTG GTGTTTACTT GATTAATTCT CCATCTCTTA TCAGTATGGA GGATAACATC 
CTGGTGTCTC GGTATATTGG CAACCCCCTT CTTATTGATG GATTTAAATT TGATGTTCGT 
CTGTATGTGC TAATCACTTC ATATGACCCT TTAGTCATTT ACCTATATGA AGAAGGACTT 
ACCAGGTTTG CCACAGCAAA GTATGACCGG GCTGCCAAAA ATATAAAAAA TCAGTTTATG 
CACCTGACTA ACTACAGTGT AAACAAGAAA AGCGGGGACT ATGTAAGTTG TGATGACCCA 
GATGTAGAAG ATTATGGGAA CAAATGGAGC ATGAGTGCCA TGCTGCGATA TTTAAAACAG 
GATGGGAAAG ATACAGCGGC TCTCATGTCA CAGGTGGAAG ATCTAATCAT TAAAACAATA 
GTTTCCGCCG AGCTTCCTAT TGCATCCGCA TGTAAATCCT TAATTACCCA CAGGGGAAAC 
TGCTTTGAAC TCTATGGGTT TGATGTGCTG ATTGATGGCA ACTTGAAGCC CTGGTTGTTG 
GAAGTGAACC TGTCTCCATC ATTAGCTTGT GATGCCCCTT TGGACTTAAA GGTGAAAGCC 
AGTATGATAT CAGACATGTT TACCCTAGTT GGTGTTGAGT GTCAGGATCC ACAACAGAGG 
TTTGGACGTG CAAGTAGTTC TTTGTATGAC AAACGAACAC AGAAATCCAC ACACCAGCGT 
CCGCTCTCTG CTAATGACAT AGACACTGGT TTGCAGGTAG GCAACAGAGA AAAGGCTGTG 
AGGCGGACAA GCTGCTTACT AGGGCTTTCC ATAGAGGAGT TGAAGATATT GCGCCGAGTT 
CAAGATGAGT ATGAGAGAAG AGGAGGGTTT GTTCGCATTT TTCCCAGGCA TAACACATGG 
CAACTTTATG GGTCTTTCCT GGAATATAAA ACCTCTCTGA ATTACATGTT AGTAACTCAT 
CTCTTCCCAA ACAGGGCTGC TGGGAATGAC CACTGTGAAA AGAATTGGGA CCCCAGGATG 
CATGCAGCTT TTTATGAACG GAAACTTGTA TCATTGCATC TTCGCAGGGC GCGGCACCGT 
GGGCTGACAC GCAAGACGGG CTTATCACAT GCTCCCCAGT GCTCTGATCA TGAACAGAGT 
AGCAAAGAGC AAGAAGAGCA GGAGGAAGAG GAGGAACTGG ATGAAAACCA CGAATTAGAG 
GTGGTGCAGG AAAAAGTTTC TTCTCCAGAC TCCTCCAAAA TAATCATACC ACCACCTCGC 
ATCAGCCTCA TGGATATTCT ACGGAAAGGT GCAGACTTCA GTAAAGTCCA GGCAAGAAAT 
GCATTCTCCT GCTACCTGCA GCGGGTGCAG AATCGTCTTC AAACTGAGCG AAACCCAGAG 
AGAGTACAAC CAAAGGAGGA GGAGCAAATA GAATTAGTGA TGCGGTTCCT CCAGCGTGGT 
GCTGCAAATT TGAAGCGGTC TCTGCCTCTG AATCTCCCAG GACAATCTGT CCCATACCTT 
GAAAGGCGCC ATTTGCTGGC AAAACTGCTT GGAGATTTTG TTGCTTTGTA TAATCAGGAA 
ACACAACAGA TGCAGAACAG TGAGGAGACA CAGAGCAATG AAGAATGTGG GGTTAATCCT 
GACGATTTTG AGGCATTTGT AGCTGATGCC AGTGAAAATG AATTGGAAGA GGTCCTCACC 
TTCTACACAC AAAAGAATAA ATCTGCAAGT GTTTTTCTTG GGACCCCAAA CAATGCAGAC 
AGGAGAGAAA CTGGGAAACC ACCAGGAGGC CCCCAGAACA GACTTACATG TGAGAGAAGC 
GTAGTTATGA GCTCAGCACC TAGCCAGCCA TCAGTCAGTA AACCAGGATA TTCTCAGGGG 
TGTAATGTGT CTGACGGTCC CATCATAATC ACTAACTCAG CAGTCAATGG GTTACCTGAA 
AGTTGCTCCC AAAGAAACAG TAGCACTCAT GTGCTTAGTA ATGATGCAAG TTTATGTTCT 
ACTATTGTTG GAAGCAATGG AATCCACGTG TCCCCTGCAA AACTTACTGT TACCCCTGGT 
TCATGGGCCA AATCTGGTTC TCGCCCTCAC TCCAGCAGCC TTGGAACATT TTCCTCCTTT 
CAAAGTGCAG CTCAGATCTA CAGCCAAAAA CTGAGACGCC CAAGCTCCAC CCGTTCAGCC 
AGCAACCATC AGAGTGCTTT ACGCTCTCGC TGTAGCTCAG CCGGATCAGT AAAAGAGCTG 
GATGTTCCAT ACAATGAAAG TGCAGTGGCC GCTTCTCTTC AGCGTCTGGC AGAGAGACAA 
TCAAGTCAAC AGTACTCCAA ACAACTTAGA CTTCTAACAC AGCAGTTAAC CAGCATGAAC 
ATAAAAGATG GTGCTTTTGG AAGTGGAAGT TTTCGTCATT GTTCTGCAAA GAGCTTCTGT 
GGAAGAGCTG TTCATGCAGG CACTGAAACT GTGGAATCAA TAACCAGAGA TATACAAAGG 
AGACGTTCTG CATGGGAAAG TGATCAAGAA AGTGGCACTT TCAGCTTTTC GTCAGATGTC 
CCTTTACAGC ATCAACCCGA TCAAATGCAG TATAGCGCAA AAGGAGGACA ACATCCCGAC 
TCTGCTATTA TCTCTCTGCC CAACCAAACC TGCACCCTTC TGCCCACCCC ACCAGTCAGT 
CACAAGCAGT CTGCAGCCAG AACATTATCC GCAACACGAT TAGTGAGGGT AGCACCAGTG 
GAGCAACATG GGACCCCCAC ATCTACAATT GTCAGTGACT TTGGAACCCC ATCACAAGGT 
TCCATGGAGG CCACTCAAAT TATATTTGCC AGAGCTAGAC CTTCAGCTCC CAAAATCGAC 
ATTAAAGGGC AAAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttll5 ( XM_012968058.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823512.1 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X1, mRNA.

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CloneID	OXa32165
Clone ID Related Accession (Same CDS sequence)	XM_012968058.2
Accession Version	XM_012968058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGTAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGCC CCCAGAACAG ACTTACATGT 
GAGAGAAGCG TAGTTATGAG CTCAGCACCT AGCCAGCCAT CAGTCAGTAA ACCAGGATAT 
TCTCAGGGGT GTAATGTGTC TGACGGTCCC ATCATAATCA CTAACTCAGC AGTCAATGGG 
TTACCTGAAA GTTGCTCCCA AAGAAACAGT AGCACTCATG TGCTTAGTAA TGATGCAAGT 
TTATGTTCTA CTATTGTTGG AAGCAATGGA ATCCACGTGT CCCCTGCAAA ACTTACTGTT 
ACCCCTGGTT CATGGGCCAA ATCTGGTTCT CGCCCTCACT CCAGCAGCCT TGGAACATTT 
TCCTCCTTTC AAAGTGCAGC TCAGATCTAC AGCCAAAAAC TGAGACGCCC AAGCTCCACC 
CGTTCAGCCA GCAACCATCA GAGTGCTTTA CGCTCTCGCT GTAGCTCAGC CGGATCAGTA 
AAAGAGCTGG ATGTTCCATA CAATGAAAGT GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA 
GAGAGACAAT CAAGTCAACA GTACTCCAAA CAACTTAGAC TTCTAACACA GCAGTTAACC 
AGCATGAACA TAAAAGATGG TGCTTTTGGA AGTGGAAGTT TTCGTCATTG TTCTGCAAAG 
AGCTTCTGTG GAAGAGCTGT TCATGCAGGC ACTGAAACTG TGGAATCAAT AACCAGAGAT 
ATACAAAGGA GACGTTCTGC ATGGGAAAGT GATCAAGAAA GTGGCACTTT CAGCTTTTCG 
TCAGATGTCC CTTTACAGCA TCAACCCGAT CAAATGTATA GCGCAAAAGG AGGACAACAT 
CCCGACTCTG CTATTATCTC TCTGCCCAAC CAAACCTGCA CCCTTCTGCC CACCCCACCA 
GTCAGTCACA AGCAGTCTGC AGCCAGAACA TTATCCGCAA CACGATTAGT GAGGGTAGCA 
CCAGTGGAGC AACATGGGAC CCCCACATCT ACAATTGTCA GTGACTTTGG AACCCCATCA 
CAAGGTTCCA TGGAGGCCAC TCAAATTATA TTTGCCAGAG CTAGACCTTC AGCTCCCAAA 
ATCGACATTA AAGGGCAAAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823512.1
CDS	142..3528
Translation	MPEMVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPHIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTHQRPLSANDIDTGLQVGNREKAVRRTSCLLGLSIEELKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADLSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGPQNRLTCERSVVMSSAPSQPSVSKPGYSQGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSASNHQSALRSRCSSAGSVKELDVPYNESAVAASLQRLAERQSSQQYSKQLRLLTQQLTSMNIKDGAFGSGSFRHCSAKSFCGRAVHAGTETVESITRDIQRRRSAWESDQESGTFSFSSDVPLQHQPDQMYSAKGGQHPDSAIISLPNQTCTLLPTPPVSHKQSAARTLSATRLVRVAPVEQHGTPTSTIVSDFGTPSQGSMEATQIIFARARPSAPKIDIKGQRK

Target ORF information:

RefSeq Version	XM_012968058.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968058.2

ORF Insert Sequence:

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGTAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGCC CCCAGAACAG ACTTACATGT 
GAGAGAAGCG TAGTTATGAG CTCAGCACCT AGCCAGCCAT CAGTCAGTAA ACCAGGATAT 
TCTCAGGGGT GTAATGTGTC TGACGGTCCC ATCATAATCA CTAACTCAGC AGTCAATGGG 
TTACCTGAAA GTTGCTCCCA AAGAAACAGT AGCACTCATG TGCTTAGTAA TGATGCAAGT 
TTATGTTCTA CTATTGTTGG AAGCAATGGA ATCCACGTGT CCCCTGCAAA ACTTACTGTT 
ACCCCTGGTT CATGGGCCAA ATCTGGTTCT CGCCCTCACT CCAGCAGCCT TGGAACATTT 
TCCTCCTTTC AAAGTGCAGC TCAGATCTAC AGCCAAAAAC TGAGACGCCC AAGCTCCACC 
CGTTCAGCCA GCAACCATCA GAGTGCTTTA CGCTCTCGCT GTAGCTCAGC CGGATCAGTA 
AAAGAGCTGG ATGTTCCATA CAATGAAAGT GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA 
GAGAGACAAT CAAGTCAACA GTACTCCAAA CAACTTAGAC TTCTAACACA GCAGTTAACC 
AGCATGAACA TAAAAGATGG TGCTTTTGGA AGTGGAAGTT TTCGTCATTG TTCTGCAAAG 
AGCTTCTGTG GAAGAGCTGT TCATGCAGGC ACTGAAACTG TGGAATCAAT AACCAGAGAT 
ATACAAAGGA GACGTTCTGC ATGGGAAAGT GATCAAGAAA GTGGCACTTT CAGCTTTTCG 
TCAGATGTCC CTTTACAGCA TCAACCCGAT CAAATGTATA GCGCAAAAGG AGGACAACAT 
CCCGACTCTG CTATTATCTC TCTGCCCAAC CAAACCTGCA CCCTTCTGCC CACCCCACCA 
GTCAGTCACA AGCAGTCTGC AGCCAGAACA TTATCCGCAA CACGATTAGT GAGGGTAGCA 
CCAGTGGAGC AACATGGGAC CCCCACATCT ACAATTGTCA GTGACTTTGG AACCCCATCA 
CAAGGTTCCA TGGAGGCCAC TCAAATTATA TTTGCCAGAG CTAGACCTTC AGCTCCCAAA 
ATCGACATTA AAGGGCAAAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttll5 ( XM_012968059.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823513.1 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa32166
Clone ID Related Accession (Same CDS sequence)	XM_012968059.2
Accession Version	XM_012968059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGTAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGGT GTAATGTGTC TGACGGTCCC 
ATCATAATCA CTAACTCAGC AGTCAATGGG TTACCTGAAA GTTGCTCCCA AAGAAACAGT 
AGCACTCATG TGCTTAGTAA TGATGCAAGT TTATGTTCTA CTATTGTTGG AAGCAATGGA 
ATCCACGTGT CCCCTGCAAA ACTTACTGTT ACCCCTGGTT CATGGGCCAA ATCTGGTTCT 
CGCCCTCACT CCAGCAGCCT TGGAACATTT TCCTCCTTTC AAAGTGCAGC TCAGATCTAC 
AGCCAAAAAC TGAGACGCCC AAGCTCCACC CGTTCAGCCA GCAACCATCA GAGTGCTTTA 
CGCTCTCGCT GTAGCTCAGC CGGATCAGTA AAAGAGCTGG ATGTTCCATA CAATGAAAGT 
GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA GAGAGACAAT CAAGTCAACA GTACTCCAAA 
CAACTTAGAC TTCTAACACA GCAGTTAACC AGCATGAACA TAAAAGATGG TGCTTTTGGA 
AGTGGAAGTT TTCGTCATTG TTCTGCAAAG AGCTTCTGTG GAAGAGCTGT TCATGCAGGC 
ACTGAAACTG TGGAATCAAT AACCAGAGAT ATACAAAGGA GACGTTCTGC ATGGGAAAGT 
GATCAAGAAA GTGGCACTTT CAGCTTTTCG TCAGATGTCC CTTTACAGCA TCAACCCGAT 
CAAATGCAGT ATAGCGCAAA AGGAGGACAA CATCCCGACT CTGCTATTAT CTCTCTGCCC 
AACCAAACCT GCACCCTTCT GCCCACCCCA CCAGTCAGTC ACAAGCAGTC TGCAGCCAGA 
ACATTATCCG CAACACGATT AGTGAGGGTA GCACCAGTGG AGCAACATGG GACCCCCACA 
TCTACAATTG TCAGTGACTT TGGAACCCCA TCACAAGGTT CCATGGAGGC CACTCAAATT 
ATATTTGCCA GAGCTAGACC TTCAGCTCCC AAAATCGACA TTAAAGGGCA AAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823513.1
CDS	142..3441
Translation	MPEMVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPHIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTHQRPLSANDIDTGLQVGNREKAVRRTSCLLGLSIEELKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADLSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSASNHQSALRSRCSSAGSVKELDVPYNESAVAASLQRLAERQSSQQYSKQLRLLTQQLTSMNIKDGAFGSGSFRHCSAKSFCGRAVHAGTETVESITRDIQRRRSAWESDQESGTFSFSSDVPLQHQPDQMQYSAKGGQHPDSAIISLPNQTCTLLPTPPVSHKQSAARTLSATRLVRVAPVEQHGTPTSTIVSDFGTPSQGSMEATQIIFARARPSAPKIDIKGQRK

Target ORF information:

RefSeq Version	XM_012968059.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968059.2

ORF Insert Sequence:

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
CACCAGCGTC CGCTCTCTGC TAATGACATA GACACTGGTT TGCAGGTAGG CAACAGAGAA 
AAGGCTGTGA GGCGGACAAG CTGCTTACTA GGGCTTTCCA TAGAGGAGTT GAAGATATTG 
CGCCGAGTTC AAGATGAGTA TGAGAGAAGA GGAGGGTTTG TTCGCATTTT TCCCAGGCAT 
AACACATGGC AACTTTATGG GTCTTTCCTG GAATATAAAA CCTCTCTGAA TTACATGTTA 
GTAACTCATC TCTTCCCAAA CAGGGCTGCT GGGAATGACC ACTGTGAAAA GAATTGGGAC 
CCCAGGATGC ATGCAGCTTT TTATGAACGG AAACTTGTAT CATTGCATCT TCGCAGGGCG 
CGGCACCGTG GGCTGACACG CAAGACGGGC TTATCACATG CTCCCCAGTG CTCTGATCAT 
GAACAGAGTA GCAAAGAGCA AGAAGAGCAG GAGGAAGAGG AGGAACTGGA TGAAAACCAC 
GAATTAGAGG TGGTGCAGGA AAAAGTTTCT TCTCCAGACT CCTCCAAAAT AATCATACCA 
CCACCTCGCA TCAGCCTCAT GGATATTCTA CGGAAAGGTG CAGACCTCAG TAAAGTCCAG 
GCAAGAAATG CATTCTCCTG CTACCTGCAG CGGGTGCAGA ATCGTCTTCA AACTGAGCGA 
AACCCAGAGA GAGTACAACC AAAGGAGGAG GAGCAAATAG AATTAGTGAT GCGGTTCCTC 
CAGCGTGGTG CTGCAAATTT GAAGCGGTCT CTGCCTCTGA ATCTCCCAGG ACAATCTGTC 
CCATACCTTG AAAGGCGCCA TTTGCTGGCA AAACTGCTTG GAGATTTTGT TGCTTTGTAT 
AATCAGGAAA CACAACAGAT GCAGAACAGT GAGGAGACAC AGAGCAATGA AGAATGTGGG 
GTTAATCCTG ACGATTTTGA GGCATTTGTA GCTGATGCCA GTGAAAATGA ATTGGAAGAG 
GTCCTCACCT TCTACACACA AAAGAATAAA TCTGCAAGTG TTTTTCTTGG GACCCCAAAC 
AATGCAGACA GGAGAGAAAC TGGGAAACCA CCAGGAGGGT GTAATGTGTC TGACGGTCCC 
ATCATAATCA CTAACTCAGC AGTCAATGGG TTACCTGAAA GTTGCTCCCA AAGAAACAGT 
AGCACTCATG TGCTTAGTAA TGATGCAAGT TTATGTTCTA CTATTGTTGG AAGCAATGGA 
ATCCACGTGT CCCCTGCAAA ACTTACTGTT ACCCCTGGTT CATGGGCCAA ATCTGGTTCT 
CGCCCTCACT CCAGCAGCCT TGGAACATTT TCCTCCTTTC AAAGTGCAGC TCAGATCTAC 
AGCCAAAAAC TGAGACGCCC AAGCTCCACC CGTTCAGCCA GCAACCATCA GAGTGCTTTA 
CGCTCTCGCT GTAGCTCAGC CGGATCAGTA AAAGAGCTGG ATGTTCCATA CAATGAAAGT 
GCAGTGGCCG CTTCTCTTCA GCGTCTGGCA GAGAGACAAT CAAGTCAACA GTACTCCAAA 
CAACTTAGAC TTCTAACACA GCAGTTAACC AGCATGAACA TAAAAGATGG TGCTTTTGGA 
AGTGGAAGTT TTCGTCATTG TTCTGCAAAG AGCTTCTGTG GAAGAGCTGT TCATGCAGGC 
ACTGAAACTG TGGAATCAAT AACCAGAGAT ATACAAAGGA GACGTTCTGC ATGGGAAAGT 
GATCAAGAAA GTGGCACTTT CAGCTTTTCG TCAGATGTCC CTTTACAGCA TCAACCCGAT 
CAAATGCAGT ATAGCGCAAA AGGAGGACAA CATCCCGACT CTGCTATTAT CTCTCTGCCC 
AACCAAACCT GCACCCTTCT GCCCACCCCA CCAGTCAGTC ACAAGCAGTC TGCAGCCAGA 
ACATTATCCG CAACACGATT AGTGAGGGTA GCACCAGTGG AGCAACATGG GACCCCCACA 
TCTACAATTG TCAGTGACTT TGGAACCCCA TCACAAGGTT CCATGGAGGC CACTCAAATT 
ATATTTGCCA GAGCTAGACC TTCAGCTCCC AAAATCGACA TTAAAGGGCA AAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttll5 ( XM_012968060.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823514.1 Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32167
Clone ID Related Accession (Same CDS sequence)	XM_012968060.2
Accession Version	XM_012968060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tubulin polyglutamylase TTLL5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
TTGAAGATAT TGCGCCGAGT TCAAGATGAG TATGAGAGAA GAGGAGGGTT TGTTCGCATT 
TTTCCCAGGC ATAACACATG GCAACTTTAT GGGTCTTTCC TGGAATATAA AACCTCTCTG 
AATTACATGT TAGTAACTCA TCTCTTCCCA AACAGGGCTG CTGGGAATGA CCACTGTGAA 
AAGAATTGGG ACCCCAGGAT GCATGCAGCT TTTTATGAAC GGAAACTTGT ATCATTGCAT 
CTTCGCAGGG CGCGGCACCG TGGGCTGACA CGCAAGACGG GCTTATCACA TGCTCCCCAG 
TGCTCTGATC ATGAACAGAG TAGCAAAGAG CAAGAAGAGC AGGAGGAAGA GGAGGAACTG 
GATGAAAACC ACGAATTAGA GGTGGTGCAG GAAAAAGTTT CTTCTCCAGA CTCCTCCAAA 
ATAATCATAC CACCACCTCG CATCAGCCTC ATGGATATTC TACGGAAAGG TGCAGACCTC 
AGTAAAGTCC AGGCAAGAAA TGCATTCTCC TGCTACCTGC AGCGGGTGCA GAATCGTCTT 
CAAACTGAGC GAAACCCAGA GAGAGTACAA CCAAAGGAGG AGGAGCAAAT AGAATTAGTG 
ATGCGGTTCC TCCAGCGTGG TGCTGCAAAT TTGAAGCGGT CTCTGCCTCT GAATCTCCCA 
GGACAATCTG TCCCATACCT TGAAAGGCGC CATTTGCTGG CAAAACTGCT TGGAGATTTT 
GTTGCTTTGT ATAATCAGGA AACACAACAG ATGCAGAACA GTGAGGAGAC ACAGAGCAAT 
GAAGAATGTG GGGTTAATCC TGACGATTTT GAGGCATTTG TAGCTGATGC CAGTGAAAAT 
GAATTGGAAG AGGTCCTCAC CTTCTACACA CAAAAGAATA AATCTGCAAG TGTTTTTCTT 
GGGACCCCAA ACAATGCAGA CAGGAGAGAA ACTGGGAAAC CACCAGGAGG CCCCCAGAAC 
AGACTTACAT GTGAGAGAAG CGTAGTTATG AGCTCAGCAC CTAGCCAGCC ATCAGTCAGT 
AAACCAGGAT ATTCTCAGGG GTGTAATGTG TCTGACGGTC CCATCATAAT CACTAACTCA 
GCAGTCAATG GGTTACCTGA AAGTTGCTCC CAAAGAAACA GTAGCACTCA TGTGCTTAGT 
AATGATGCAA GTTTATGTTC TACTATTGTT GGAAGCAATG GAATCCACGT GTCCCCTGCA 
AAACTTACTG TTACCCCTGG TTCATGGGCC AAATCTGGTT CTCGCCCTCA CTCCAGCAGC 
CTTGGAACAT TTTCCTCCTT TCAAAGTGCA GCTCAGATCT ACAGCCAAAA ACTGAGACGC 
CCAAGCTCCA CCCGTTCAGC CAGCAACCAT CAGAGTGCTT TACGCTCTCG CTGTAGCTCA 
GCCGGATCAG TAAAAGAGCT GGATGTTCCA TACAATGAAA GTGCAGTGGC CGCTTCTCTT 
CAGCGTCTGG CAGAGAGACA ATCAAGTCAA CAGTACTCCA AACAACTTAG ACTTCTAACA 
CAGCAGTTAA CCAGCATGAA CATAAAAGAT GGTGCTTTTG GAAGTGGAAG TTTTCGTCAT 
TGTTCTGCAA AGAGCTTCTG TGGAAGAGCT GTTCATGCAG GCACTGAAAC TGTGGAATCA 
ATAACCAGAG ATATACAAAG GAGACGTTCT GCATGGGAAA GTGATCAAGA AAGTGGCACT 
TTCAGCTTTT CGTCAGATGT CCCTTTACAG CATCAACCCG ATCAAATGCA GTATAGCGCA 
AAAGGAGGAC AACATCCCGA CTCTGCTATT ATCTCTCTGC CCAACCAAAC CTGCACCCTT 
CTGCCCACCC CACCAGTCAG TCACAAGCAG TCTGCAGCCA GAACATTATC CGCAACACGA 
TTAGTGAGGG TAGCACCAGT GGAGCAACAT GGGACCCCCA CATCTACAAT TGTCAGTGAC 
TTTGGAACCC CATCACAAGG TTCCATGGAG GCCACTCAAA TTATATTTGC CAGAGCTAGA 
CCTTCAGCTC CCAAAATCGA CATTAAAGGG CAAAGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823514.1
CDS	142..3423
Translation	MPEMVPRGQQDEQSEEDDDSKKGEYSCILWAGGSRKVPIVMFHAEAVLHKNLSLRAVGERYKLSYKIVRTDSRLVRSILSAHGFQEVNANSNDFNIMWTGSHVKPHIMRSLTNFQKVNHFPRSYELTRKDRLYKNVQRMQQSHGFKNFHLLPQTYLLPAEYQDFCTAFAKDRGPWIVKPVASSRGRGVYLINSPSLISMEDNILVSRYIGNPLLIDGFKFDVRLYVLITSYDPLVIYLYEEGLTRFATAKYDRAAKNIKNQFMHLTNYSVNKKSGDYVSCDDPDVEDYGNKWSMSAMLRYLKQDGKDTAALMSQVEDLIIKTIVSAELPIASACKSLITHRGNCFELYGFDVLIDGNLKPWLLEVNLSPSLACDAPLDLKVKASMISDMFTLVGVECQDPQQRFGRASSSLYDKRTQKSTLKILRRVQDEYERRGGFVRIFPRHNTWQLYGSFLEYKTSLNYMLVTHLFPNRAAGNDHCEKNWDPRMHAAFYERKLVSLHLRRARHRGLTRKTGLSHAPQCSDHEQSSKEQEEQEEEEELDENHELEVVQEKVSSPDSSKIIIPPPRISLMDILRKGADLSKVQARNAFSCYLQRVQNRLQTERNPERVQPKEEEQIELVMRFLQRGAANLKRSLPLNLPGQSVPYLERRHLLAKLLGDFVALYNQETQQMQNSEETQSNEECGVNPDDFEAFVADASENELEEVLTFYTQKNKSASVFLGTPNNADRRETGKPPGGPQNRLTCERSVVMSSAPSQPSVSKPGYSQGCNVSDGPIIITNSAVNGLPESCSQRNSSTHVLSNDASLCSTIVGSNGIHVSPAKLTVTPGSWAKSGSRPHSSSLGTFSSFQSAAQIYSQKLRRPSSTRSASNHQSALRSRCSSAGSVKELDVPYNESAVAASLQRLAERQSSQQYSKQLRLLTQQLTSMNIKDGAFGSGSFRHCSAKSFCGRAVHAGTETVESITRDIQRRRSAWESDQESGTFSFSSDVPLQHQPDQMQYSAKGGQHPDSAIISLPNQTCTLLPTPPVSHKQSAARTLSATRLVRVAPVEQHGTPTSTIVSDFGTPSQGSMEATQIIFARARPSAPKIDIKGQRK

Target ORF information:

RefSeq Version	XM_012968060.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tubulin tyrosine ligase like 5 (ttll5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968060.2

ORF Insert Sequence:

ATGCCTGAAA TGGTGCCCCG TGGACAGCAG GACGAACAGA GCGAGGAAGA TGATGATTCC 
AAAAAGGGTG AATACTCTTG CATTCTGTGG GCTGGTGGCT CTCGAAAGGT TCCTATTGTA 
ATGTTTCATG CAGAAGCCGT TCTTCACAAA AACCTGTCAC TGCGTGCAGT AGGAGAGCGC 
TATAAATTAT CCTATAAGAT TGTTCGCACA GACAGCCGCT TAGTACGCAG CATTCTTTCT 
GCCCATGGAT TTCAGGAGGT AAATGCCAAC AGCAATGACT TCAACATTAT GTGGACTGGG 
TCCCACGTAA AGCCTCACAT CATGAGGAGC CTTACCAACT TTCAAAAAGT CAATCACTTT 
CCCAGATCCT ATGAGCTGAC CCGTAAGGAC CGACTGTACA AAAATGTGCA GCGCATGCAG 
CAAAGTCATG GCTTTAAAAA CTTCCACCTT TTGCCCCAGA CATATCTGCT ACCTGCTGAA 
TATCAGGACT TTTGCACTGC TTTTGCCAAA GATCGTGGTC CTTGGATAGT GAAGCCAGTT 
GCCTCATCAC GTGGCCGTGG TGTTTACTTG ATTAATTCTC CATCTCTTAT CAGTATGGAG 
GATAACATCC TGGTGTCTCG GTATATTGGC AACCCCCTTC TTATTGATGG ATTTAAATTT 
GATGTTCGTC TGTATGTGCT AATCACTTCA TATGACCCTT TAGTCATTTA CCTATATGAA 
GAAGGACTTA CCAGGTTTGC CACAGCAAAG TATGACCGGG CTGCCAAAAA TATAAAAAAT 
CAGTTTATGC ACCTGACTAA CTACAGTGTA AACAAGAAAA GCGGGGACTA TGTAAGTTGT 
GATGACCCAG ATGTAGAAGA TTATGGGAAC AAATGGAGCA TGAGTGCCAT GCTGCGATAT 
TTAAAACAGG ATGGGAAAGA TACAGCGGCT CTCATGTCAC AGGTGGAAGA TCTAATCATT 
AAAACAATAG TTTCCGCCGA GCTTCCTATT GCATCCGCAT GTAAATCCTT AATTACCCAC 
AGGGGAAACT GCTTTGAACT CTATGGGTTT GATGTGCTGA TTGATGGCAA CTTGAAGCCC 
TGGTTGTTGG AAGTGAACCT GTCTCCATCA TTAGCTTGTG ATGCCCCTTT GGACTTAAAG 
GTGAAAGCCA GTATGATATC AGACATGTTT ACCCTAGTTG GTGTTGAGTG TCAGGATCCA 
CAACAGAGGT TTGGACGTGC AAGTAGTTCT TTGTATGACA AACGAACACA GAAATCCACA 
TTGAAGATAT TGCGCCGAGT TCAAGATGAG TATGAGAGAA GAGGAGGGTT TGTTCGCATT 
TTTCCCAGGC ATAACACATG GCAACTTTAT GGGTCTTTCC TGGAATATAA AACCTCTCTG 
AATTACATGT TAGTAACTCA TCTCTTCCCA AACAGGGCTG CTGGGAATGA CCACTGTGAA 
AAGAATTGGG ACCCCAGGAT GCATGCAGCT TTTTATGAAC GGAAACTTGT ATCATTGCAT 
CTTCGCAGGG CGCGGCACCG TGGGCTGACA CGCAAGACGG GCTTATCACA TGCTCCCCAG 
TGCTCTGATC ATGAACAGAG TAGCAAAGAG CAAGAAGAGC AGGAGGAAGA GGAGGAACTG 
GATGAAAACC ACGAATTAGA GGTGGTGCAG GAAAAAGTTT CTTCTCCAGA CTCCTCCAAA 
ATAATCATAC CACCACCTCG CATCAGCCTC ATGGATATTC TACGGAAAGG TGCAGACCTC 
AGTAAAGTCC AGGCAAGAAA TGCATTCTCC TGCTACCTGC AGCGGGTGCA GAATCGTCTT 
CAAACTGAGC GAAACCCAGA GAGAGTACAA CCAAAGGAGG AGGAGCAAAT AGAATTAGTG 
ATGCGGTTCC TCCAGCGTGG TGCTGCAAAT TTGAAGCGGT CTCTGCCTCT GAATCTCCCA 
GGACAATCTG TCCCATACCT TGAAAGGCGC CATTTGCTGG CAAAACTGCT TGGAGATTTT 
GTTGCTTTGT ATAATCAGGA AACACAACAG ATGCAGAACA GTGAGGAGAC ACAGAGCAAT 
GAAGAATGTG GGGTTAATCC TGACGATTTT GAGGCATTTG TAGCTGATGC CAGTGAAAAT 
GAATTGGAAG AGGTCCTCAC CTTCTACACA CAAAAGAATA AATCTGCAAG TGTTTTTCTT 
GGGACCCCAA ACAATGCAGA CAGGAGAGAA ACTGGGAAAC CACCAGGAGG CCCCCAGAAC 
AGACTTACAT GTGAGAGAAG CGTAGTTATG AGCTCAGCAC CTAGCCAGCC ATCAGTCAGT 
AAACCAGGAT ATTCTCAGGG GTGTAATGTG TCTGACGGTC CCATCATAAT CACTAACTCA 
GCAGTCAATG GGTTACCTGA AAGTTGCTCC CAAAGAAACA GTAGCACTCA TGTGCTTAGT 
AATGATGCAA GTTTATGTTC TACTATTGTT GGAAGCAATG GAATCCACGT GTCCCCTGCA 
AAACTTACTG TTACCCCTGG TTCATGGGCC AAATCTGGTT CTCGCCCTCA CTCCAGCAGC 
CTTGGAACAT TTTCCTCCTT TCAAAGTGCA GCTCAGATCT ACAGCCAAAA ACTGAGACGC 
CCAAGCTCCA CCCGTTCAGC CAGCAACCAT CAGAGTGCTT TACGCTCTCG CTGTAGCTCA 
GCCGGATCAG TAAAAGAGCT GGATGTTCCA TACAATGAAA GTGCAGTGGC CGCTTCTCTT 
CAGCGTCTGG CAGAGAGACA ATCAAGTCAA CAGTACTCCA AACAACTTAG ACTTCTAACA 
CAGCAGTTAA CCAGCATGAA CATAAAAGAT GGTGCTTTTG GAAGTGGAAG TTTTCGTCAT 
TGTTCTGCAA AGAGCTTCTG TGGAAGAGCT GTTCATGCAG GCACTGAAAC TGTGGAATCA 
ATAACCAGAG ATATACAAAG GAGACGTTCT GCATGGGAAA GTGATCAAGA AAGTGGCACT 
TTCAGCTTTT CGTCAGATGT CCCTTTACAG CATCAACCCG ATCAAATGCA GTATAGCGCA 
AAAGGAGGAC AACATCCCGA CTCTGCTATT ATCTCTCTGC CCAACCAAAC CTGCACCCTT 
CTGCCCACCC CACCAGTCAG TCACAAGCAG TCTGCAGCCA GAACATTATC CGCAACACGA 
TTAGTGAGGG TAGCACCAGT GGAGCAACAT GGGACCCCCA CATCTACAAT TGTCAGTGAC 
TTTGGAACCC CATCACAAGG TTCCATGGAG GCCACTCAAA TTATATTTGC CAGAGCTAGA 
CCTTCAGCTC CCAAAATCGA CATTAAAGGG CAAAGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ttll5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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