gen1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following gen1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gen1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24185 NM_001126779.1
Latest version!
Xenopus tropicalis GEN1, Holliday junction 5' flap endonuclease (gen1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa47280 NM_001374837.1
Latest version!
Xenopus tropicalis GEN1, Holliday junction 5' flap endonuclease (gen1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa57879 XM_031901515.1
Latest version!
Xenopus tropicalis GEN1, Holliday junction 5' flap endonuclease (gen1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24185
    Clone ID Related Accession (Same CDS sequence) NM_001126779.1
    Accession Version NM_001126779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-28
    Organism Xenopus tropicalis(tropical clawed frog)
    Product flap endonuclease GEN homolog 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC160467.1. ##Evidence-Data-START## Transcript exon combination :: BC160467.1, CX483614.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025687, SAMEA1696413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGAGTCA CTGATCTGTG GTCCATCCTG GGGCCAGTGA AGAAGCACGT GCCCTTAGAG 
    AGCCTCGCTG GGAAAACGCT GGCCGTCGAT CTGAGTATCT GGGTGTGTGA AGCGCAAATG
    GTAAAACAGA TGATCGGAGT GGTCCACAAA CCCCATCTCA GGAATCTGTT CTTCCGCATC
    TCTTCGCTGA ATCTATTGGG AGTGAAGCTG GTGTTTGTTT CGGAGGGTGA AGCCCCAAAG
    ATTAAAGCTG AAACCATGAG CAAGAGGAAT GAGATGCGCT ATGGGCCTTC TGCCAGTGCT
    GCACCGCCCA AAGCAGGAAG ATCCTATTTT AAGTCTGTTT TAAAGGAGTG CCTGCTGATG
    CTGGAGTGTT TGGGCATCCC TTGGGTACAA GCAGCTGGGG AAGCCGAGGC CATGTGTGCC
    TACCTGAATG CCCATGGCTA CGTGGATGGA TGCATTACCA ACGATGGCGA TGTCTTCTTG
    TATGGGGCCC AGACATTTTA TCGGAATTTC ACTATGAATG TGAAGGACCC CCATGTGGAT
    TGCTATGAAG TATCCAAAAT TAAGGCCCAG CTTGGTTTGG ACAGAGAGGA ACTGGTAGGC
    TTGGCCATCC TCCTGGGCTG TGATTATCTC CCGAAAGTGA GTGCTTGCTT TGTTTATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120251.1
    CDS180..839
    Translation

    Target ORF information:

    RefSeq Version NM_001126779.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GEN1, Holliday junction 5' flap endonuclease (gen1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001126779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGGAGTCA CTGATCTGTG GTCCATCCTG GGGCCAGTGA AGAAGCACGT GCCCTTAGAG 
    AGCCTCGCTG GGAAAACGCT GGCCGTCGAT CTGAGTATCT GGGTGTGTGA AGCGCAAATG
    GTAAAACAGA TGATCGGAGT GGTCCACAAA CCCCATCTCA GGAATCTGTT CTTCCGCATC
    TCTTCGCTGA ATCTATTGGG AGTGAAGCTG GTGTTTGTTT CGGAGGGTGA AGCCCCAAAG
    ATTAAAGCTG AAACCATGAG CAAGAGGAAT GAGATGCGCT ATGGGCCTTC TGCCAGTGCT
    GCACCGCCCA AAGCAGGAAG ATCCTATTTT AAGTCTGTTT TAAAGGAGTG CCTGCTGATG
    CTGGAGTGTT TGGGCATCCC TTGGGTACAA GCAGCTGGGG AAGCCGAGGC CATGTGTGCC
    TACCTGAATG CCCATGGCTA CGTGGATGGA TGCATTACCA ACGATGGCGA TGTCTTCTTG
    TATGGGGCCC AGACATTTTA TCGGAATTTC ACTATGAATG TGAAGGACCC CCATGTGGAT
    TGCTATGAAG TATCCAAAAT TAAGGCCCAG CTTGGTTTGG ACAGAGAGGA ACTGGTAGGC
    TTGGCCATCC TCCTGGGCTG TGATTATCTC CCGAAAGTGA GTGCTTGCTT TGTTTATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47280
    Clone ID Related Accession (Same CDS sequence) NM_001374837.1
    Accession Version NM_001374837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-16
    Organism Xenopus tropicalis(tropical clawed frog)
    Product flap endonuclease GEN homolog 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03021419.1 and AAMC03021420.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1696413, SAMEA1696414 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGAGTCA CTGATCTGTG GTCCATCCTG GGGCCAGTGA AGAAGCACGT GCCCTTAGAG 
    AGCCTCGCTG GGAAAACGCT GGCCGTTGAT CTGAGTATCT GGGTGTGTGA AGCACAAATG
    GTAAAACAGA TGATCGGAGT GGTCCACAAA CCCCATCTCA GGAATCTGTT CTTCCGCATC
    TCATCACTGA ATCTATTGGG AGTGAAGCTG GTGTTTGTTT CGGAGGGTGA AGCCCCAAAG
    ATTAAAGCTG AAACCATGAG CAAGAGGAAT GAGATGCGCT ACGGGCCTTC TGCCAGTGCT
    GCACCGCCCA AAGCAGGAAG GTCCTATTTT AAGTCTGTTT TAAAGGAGTG CCTGCTGATG
    CTGGAGTGTT TGGGCATCCC TTGGGTACAA GCAGCTGGGG AAGCCGAGGC CATGTGTGCC
    TACCTGAATG CCCATGGCTA CGTGGATGGA TGCATTACCA ACGATGGCGA TGTCTTCTTG
    TATGGGGCCC AGACAGTTTA TCGGAATTTC ACTATGAATG TGAAGGACCC CCATGTGGAT
    TGCTATGAAG TATCCAAAAT TAAGGCCCAG CTTGGTTTGG ACAGAGAGGA ACTGGTAGGC
    TTGGCCATCC TCCTGGGCTG TGATTATCTC CCGAAAGGGG TGCCTGGAGT CCGAAAAGTA
    CAAGCCCTAA AGCTTATTGA GATGCTAAAT GGGGAGAGTT TGCTGCAAAG GTTCTATCAG
    TGGGAGAAGG ACTGTAACGA ACGGGGCACT AAAGCCCCTA AGAAAAAACC TCACTGTTCT
    GTTTGCTGCC ATCCAGGTTC AGCTAAAGAT CACGAGCGCA ATGGCTGTAA GATGTGCGGG
    AGCGATCGAT TCTGCGAACC ACATGACTAT GATTATCGCT GCCCATGTGA CTGGCATAAA
    GAACAGAGAG AGAAGCAGGA AAATTCCGTG GAGTTCAATA TCAAGAGGAA GTCTCTCCGA
    TGTGAAGGTT TCCCATACCG CCAGGTCATT GATGAATTTC TTATTAATAA GAATAAATTG
    GTGAAAGTGC TTAAATGGGG AAGACCCAGT CTACAGTGTT TTCAGAATTT TACGGCTGAG
    CGAATGGATT GGCCCAGGCA TTACTCGTGT GAGAAGTTGC TTGTGCTGTT GACGTATTAC
    GACATGAATG AGAGAAAGGC AGGCAGAGAG AATGGGACGC AGCTACAGGC TGTACGCATT
    GTTAAGACAA GAATCAGGAA TGGAAGCCCT TGCTTTGAAA TCGAGTGGGT GAAACCAGAC
    AGTTATGTTT TTGCCGATGA TCATCCCGCT GAGTCTCCTC TGGTGACGAT AGAGGAAGAA
    TCTCTCTTCC GAGCAGCTTA CCCCCATGTT GTCACCATGT TTCACAAGGA GAAGCAAGAA
    GCTGAGGAGA ATAAGCTGAA ATGCAAGAAA AGCAAGCCCA AATCCAAGGC TGCGGATGAT
    CTGGATGATG TTGCGTCTCT GTTGTCCGAA CTGAGCCTGA AACCTGTTTC GGAAAAGCAG
    CCTCCACTAG AGCTACAGGA AGATGGAGGA GACTCGGACA GTGTTCATGA GAAGCCAGCA
    AGGGATTCCA ACTTTCTTAG CTTGTCCAGC GATGCCGATT CTGGAGTGGA AAGAGGGATT
    TTGTTAGATA CCAAAACAGA TTCTGAGTTT TTCACATCAC CCACTGCAGA CAATGAGCCT
    TCCAATCCCT TGTGTTCCTC TCCAGCACCA AGCAATACCC AACATCTTAC TGCTGCTTCT
    CCTAATATTT CTGCCGTGAT TTCAGAACTC CAACTCAGCA GTATCGACTG GGACTCAACC
    TCTTTTAGCA AGTCTCCCCA AGCTGAAGAA CGCACTAAAG GCAGCAAAGA GGTTGGAGAA
    CGGGGGGAGT TTTGTTACAA ACCAACCACC AACAACTTGG AAACTTCAGG AAATAAATCT
    AAGGAGGTTC AGAATGGCAT AGCCAGGCCT GGAACCTCTA ATAAGGTGCA CTTGACTAAT
    TCAAAGCAAT CCAATGTGGA AGACTTTCAT AAGTTACCTT TAAAAGAAAG AGTCCTTCTA
    AAGAATGCTG TTCAGTCTGG TGTCCCTTAT CCAGGAAAAA TGCAAACTAA ACCTGTTCCC
    GTAAAGCCTG TGCCACTATC TCTCCATAAC CAAGGGCTCG GTAATCACAG CTCAAAAAAC
    TCTGTTCCCA CCACTTTGGG TATAGACATT AAGGAAAAAG TACATCAGAA ACTCCCAATG
    AAGTTATCTA GAGATCCGAG TCAACAAGAG AGCAGGCAAC CACCAACTTC AGTCTGTTCC
    AGAGCTAAGC CTCAGAATCA GGGAGCTACT GGGCCAAAAA CATACAATTT TGTTAAGAAT
    GTGAAGGCAG TGCCACTCGT CCCTGTGAAC ACTGGTAATA AGATGGGGAC CCTGGCAGCT
    TCTAAATCCT TAGGGAAGAA GAGCGTCTGT CAAAAAGGGA CCTTATATGA CCAGGAAGAA
    AAAACATACC AACATAAAAT GCAGGCAACA TGCCCAAGCG CAATTGTTCA TCCTCACTCC
    AATGGGGTTG AGGGGGCTAA ATTACATAGA CCACCATATG GGGACAAAGA AGTAAAGCAG
    AACAAAGAGA ACTCCCCTCC AAGAGATAAT CTCATTTGCC ATTCTGGTTT AAACCCTATT
    TTCCTTAGCC CTGACTCTAA GGCAGCCACC AAAAAAGCAA GTGACTGTGC CGCCGAGGAC
    AGCGACGGAG ACGACTCTAT TATATCTGTA GACAGCCCCT TGCCCCTGGC TGAACGTTTA
    AAGCTAAGAT TGTTACAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361766.1
    CDS200..2983
    Translation

    Target ORF information:

    RefSeq Version NM_001374837.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GEN1, Holliday junction 5' flap endonuclease (gen1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGAGTCA CTGATCTGTG GTCCATCCTG GGGCCAGTGA AGAAGCACGT GCCCTTAGAG 
    AGCCTCGCTG GGAAAACGCT GGCCGTTGAT CTGAGTATCT GGGTGTGTGA AGCACAAATG
    GTAAAACAGA TGATCGGAGT GGTCCACAAA CCCCATCTCA GGAATCTGTT CTTCCGCATC
    TCATCACTGA ATCTATTGGG AGTGAAGCTG GTGTTTGTTT CGGAGGGTGA AGCCCCAAAG
    ATTAAAGCTG AAACCATGAG CAAGAGGAAT GAGATGCGCT ACGGGCCTTC TGCCAGTGCT
    GCACCGCCCA AAGCAGGAAG GTCCTATTTT AAGTCTGTTT TAAAGGAGTG CCTGCTGATG
    CTGGAGTGTT TGGGCATCCC TTGGGTACAA GCAGCTGGGG AAGCCGAGGC CATGTGTGCC
    TACCTGAATG CCCATGGCTA CGTGGATGGA TGCATTACCA ACGATGGCGA TGTCTTCTTG
    TATGGGGCCC AGACAGTTTA TCGGAATTTC ACTATGAATG TGAAGGACCC CCATGTGGAT
    TGCTATGAAG TATCCAAAAT TAAGGCCCAG CTTGGTTTGG ACAGAGAGGA ACTGGTAGGC
    TTGGCCATCC TCCTGGGCTG TGATTATCTC CCGAAAGGGG TGCCTGGAGT CCGAAAAGTA
    CAAGCCCTAA AGCTTATTGA GATGCTAAAT GGGGAGAGTT TGCTGCAAAG GTTCTATCAG
    TGGGAGAAGG ACTGTAACGA ACGGGGCACT AAAGCCCCTA AGAAAAAACC TCACTGTTCT
    GTTTGCTGCC ATCCAGGTTC AGCTAAAGAT CACGAGCGCA ATGGCTGTAA GATGTGCGGG
    AGCGATCGAT TCTGCGAACC ACATGACTAT GATTATCGCT GCCCATGTGA CTGGCATAAA
    GAACAGAGAG AGAAGCAGGA AAATTCCGTG GAGTTCAATA TCAAGAGGAA GTCTCTCCGA
    TGTGAAGGTT TCCCATACCG CCAGGTCATT GATGAATTTC TTATTAATAA GAATAAATTG
    GTGAAAGTGC TTAAATGGGG AAGACCCAGT CTACAGTGTT TTCAGAATTT TACGGCTGAG
    CGAATGGATT GGCCCAGGCA TTACTCGTGT GAGAAGTTGC TTGTGCTGTT GACGTATTAC
    GACATGAATG AGAGAAAGGC AGGCAGAGAG AATGGGACGC AGCTACAGGC TGTACGCATT
    GTTAAGACAA GAATCAGGAA TGGAAGCCCT TGCTTTGAAA TCGAGTGGGT GAAACCAGAC
    AGTTATGTTT TTGCCGATGA TCATCCCGCT GAGTCTCCTC TGGTGACGAT AGAGGAAGAA
    TCTCTCTTCC GAGCAGCTTA CCCCCATGTT GTCACCATGT TTCACAAGGA GAAGCAAGAA
    GCTGAGGAGA ATAAGCTGAA ATGCAAGAAA AGCAAGCCCA AATCCAAGGC TGCGGATGAT
    CTGGATGATG TTGCGTCTCT GTTGTCCGAA CTGAGCCTGA AACCTGTTTC GGAAAAGCAG
    CCTCCACTAG AGCTACAGGA AGATGGAGGA GACTCGGACA GTGTTCATGA GAAGCCAGCA
    AGGGATTCCA ACTTTCTTAG CTTGTCCAGC GATGCCGATT CTGGAGTGGA AAGAGGGATT
    TTGTTAGATA CCAAAACAGA TTCTGAGTTT TTCACATCAC CCACTGCAGA CAATGAGCCT
    TCCAATCCCT TGTGTTCCTC TCCAGCACCA AGCAATACCC AACATCTTAC TGCTGCTTCT
    CCTAATATTT CTGCCGTGAT TTCAGAACTC CAACTCAGCA GTATCGACTG GGACTCAACC
    TCTTTTAGCA AGTCTCCCCA AGCTGAAGAA CGCACTAAAG GCAGCAAAGA GGTTGGAGAA
    CGGGGGGAGT TTTGTTACAA ACCAACCACC AACAACTTGG AAACTTCAGG AAATAAATCT
    AAGGAGGTTC AGAATGGCAT AGCCAGGCCT GGAACCTCTA ATAAGGTGCA CTTGACTAAT
    TCAAAGCAAT CCAATGTGGA AGACTTTCAT AAGTTACCTT TAAAAGAAAG AGTCCTTCTA
    AAGAATGCTG TTCAGTCTGG TGTCCCTTAT CCAGGAAAAA TGCAAACTAA ACCTGTTCCC
    GTAAAGCCTG TGCCACTATC TCTCCATAAC CAAGGGCTCG GTAATCACAG CTCAAAAAAC
    TCTGTTCCCA CCACTTTGGG TATAGACATT AAGGAAAAAG TACATCAGAA ACTCCCAATG
    AAGTTATCTA GAGATCCGAG TCAACAAGAG AGCAGGCAAC CACCAACTTC AGTCTGTTCC
    AGAGCTAAGC CTCAGAATCA GGGAGCTACT GGGCCAAAAA CATACAATTT TGTTAAGAAT
    GTGAAGGCAG TGCCACTCGT CCCTGTGAAC ACTGGTAATA AGATGGGGAC CCTGGCAGCT
    TCTAAATCCT TAGGGAAGAA GAGCGTCTGT CAAAAAGGGA CCTTATATGA CCAGGAAGAA
    AAAACATACC AACATAAAAT GCAGGCAACA TGCCCAAGCG CAATTGTTCA TCCTCACTCC
    AATGGGGTTG AGGGGGCTAA ATTACATAGA CCACCATATG GGGACAAAGA AGTAAAGCAG
    AACAAAGAGA ACTCCCCTCC AAGAGATAAT CTCATTTGCC ATTCTGGTTT AAACCCTATT
    TTCCTTAGCC CTGACTCTAA GGCAGCCACC AAAAAAGCAA GTGACTGTGC CGCCGAGGAC
    AGCGACGGAG ACGACTCTAT TATATCTGTA GACAGCCCCT TGCCCCTGGC TGAACGTTTA
    AAGCTAAGAT TGTTACAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57879
    Clone ID Related Accession (Same CDS sequence) XM_031901515.1
    Accession Version XM_031901515.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product flap endonuclease GEN homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGAGTCA CTGATCTGTG GTCCATCCTG GGGCCAGTGA AGAAGCACGT GCCCTTAGAG 
    AGCCTCGCTG GGAAAACGCT GGCCGTTGAT CTGAGTATCT GGGTGTGTGA AGCACAAATG
    GTAAAACAGA TGATCGGAGT GGTCCACAAA CCCCATCTCA GGAATCTGTT CTTCCGCATC
    TCATCACTGA ATCTATTGGG AGTGAAGCTG GTGTTTGTTT CGGAGGGTGA AGCCCCAAAG
    ATTAAAGCTG AAACCATGAG CAAGAGGAAT GAGATGCGCT ACGGGCCTTC TGCCAGTGCT
    GCACCGCCCA AAGCAGGAAG GTCCTATTTT AAGTCTGTTT TAAAGGAGTG CCTGCTGATG
    CTGGAGTGTT TGGGCATCCC TTGGGTACAA GCAGCTGGGG AAGCCGAGGC CATGTGTGCC
    TACCTGAATG CCCATGGCTA CGTGGATGGA TGCATTACCA ACGATGGCGA TGTCTTCTTG
    TATGGGGCCC AGACAGTTTA TCGGAATTTC ACTATGAATG TGAAGGACCC CCATGTGGAT
    TGCTATGAAG TATCCAAAAT TAAGGCCCAG CTTGGTTTGG ACAGAGAGGA ACTGGTAGGC
    TTGGCCATCC TCCTGGGCTG TGATTATCTC CCGAAAGGGG TGCCTGGAGT CCGAAAAGTA
    CAAGCCCTAA AGCTTATTGA GATGCTAAAT GGGGAGAGTT TGCTGCAAAG GTTCTATCAG
    TGGGAGAAGG ACTGTAACGA ACGGGGCACT AAAGCCCCTA AGAAAAAACC TCACTGTTCT
    GTTTGCTGCC ATCCAGGTTC AGCTAAAGAT CACGAGCGCA ATGGCTGTAA GATGTGCGGG
    AGCGATCGAT TCTGCGAACC ACATGACTAT GATTATCGCT GCCCATGTGA CTGGCATAAA
    GAACAGAGAG AGAAGCAGGA AAATTCTGTG GAGTTTAATA TCAAGAGGAA GTCTCTCCGA
    TGTGAAGGTT TCCCATACCG CCAGGTCATT GATGAATTTC TTATTAATAA GAATAAATTG
    GTGAAAGTGC TTAAATGGGG AAGACCCAGT CTACAGTGTT TTCAGAATTT TACGGCTGAG
    CGAATGGATT GGCCCAGGCA TTACTCGTGT GAGAAGTTGC TTGTGCTGTT GACGTACTAC
    GACATGAATG AGAGAAAGGC AGGCAGAGAG AATGGGGCGC AGCTACAGGC TGTACGCATT
    GTTAAAACAA GAATCAGGAA TGGAAGCCCT TGCTTTGAAA TCGAGTGGGT GAAACCAGAC
    AGTTATGCTT TTGCCGATGA TCATCCCGCT GAGTCTCCTC TGGTGACGAT AGAGGAAGAA
    TCTCTCTTCC GAGCAGCTTA CCCCCATGTT GTCACCATGT TTCACAAGGA GAAGCAAGAA
    GCTGAGGAGA ATAAGCTGAA ATGCAAGAAA AGCAAGCCCA AATCCAAGGC TGCGGATGAT
    CTGGATGATG TTGCGTCTCT GTTGTCCGAA CTGAGCCTGA AACCTGTTTC GGAAAAGCAG
    CCTCCACTAG AGCTACAGGA AGATGGAGGA GACTCGGACA GTGTTCATGA GAAGCCAGCA
    AGGGATTCCA ACTTTCTTAG CTTGTCCAGC GATGCCGATT CTGGAGTGGA AAGAGGGATT
    TTGTTAGATA CCAAAACAGA TTCTGAGTTT TTCACATCAC CCACTGCAGA CAATGAGCCT
    TCCAATCCCT TGTGTTCCTC TCCAGCACCA AGCAATACCC AACATCTTAC TGCTGCTTCT
    CCTAATATTT CTGCCGTGAT TTCAGAACTC CAACTCAGCA GTATCGACTG GGACTCAACC
    TCTTTTAGCA AGTCTCCCCA AGCTGAAGAA CGCACTAAAG GCAGCAAAGA GGTTGGAGAA
    CGGGGGGAGT TTTGTTACAA ACCAACCACC AACAACTTGG AAACTTCAGG AAATAAATCT
    AAGGAGGTTC AGAATGGCAT AGCCAGGCCT GGAACCTCTA ATAAGGTGCA CTTGACTAAT
    TCAAAGCAAT CCAATGTGGA AGACTTTCAT AAGTTACCTT TAAAAGAAAG AGTCCTTCTA
    AAGAATGCTG TTCAGTCTGG TGTCCCTTAT CCAGGAAAAA TGCAAACTAA ACCTGTTCCC
    GTAAAGCCTG TGCCACTATC TCTCCATAAC CAAGGGCTCG GTAATCACAG CTCAAAAAAC
    TCTGTTCCCA CCACTTTGGG TATAGACATT AAGGAAAAAG TACATCAGAA ACTCCCAATG
    AAGTTATCTA GAGATCCGAG TCAACAAGAG AGCAGGCAAC CACCAACTTC AGTCTGTTCC
    AGAGCTAAGC CTCAGAATCA GGGAGCTACT GGGCCAAAAA CATACAATTT TGTTAAGAAT
    GTGAAGGCAG TGCCACTCGT CCCTGTGAAC ACTGGTAATA AGATGGGGAC CCTGGCAGCT
    TCTAAATCCT TAGGGAAGAA GAGCGTCTGT CAAAAAGGGA CCTTATATGA CCAGGAAGAA
    AAAACATACC AACATAAAAT GCAGGCAACA TGCCCAAGCG CAATTGTTCA TCCTCACTCC
    AATGGGGTTG AGGGGGCTAA ATTACATAGA CCACCATATG GGGACAAAGA AGTAAAGCAG
    AACAAAGAGA ACTCCCCTCC AAGAGATAAT CTCATTTGCC ATTCTGGTTT AAACCCTATT
    TTCCTTAGCC CTGACTCTAA GGCAGCCACC AAAAAAGCAA GTGACTGTGC CGCCGAGGAC
    AGCGACGGAG ACGACTCTAT TATATCTGTA GACAGCCCCT TGCCCCTGGC TGAACGTTTA
    AAGCTAAGAT TGTTACAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757375.1
    CDS48..2831
    Translation

    Target ORF information:

    RefSeq Version XM_031901515.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis GEN1, Holliday junction 5' flap endonuclease (gen1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901515.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGGAGTCA CTGATCTGTG GTCCATCCTG GGGCCAGTGA AGAAGCACGT GCCCTTAGAG 
    AGCCTCGCTG GGAAAACGCT GGCCGTTGAT CTGAGTATCT GGGTGTGTGA AGCACAAATG
    GTAAAACAGA TGATCGGAGT GGTCCACAAA CCCCATCTCA GGAATCTGTT CTTCCGCATC
    TCATCACTGA ATCTATTGGG AGTGAAGCTG GTGTTTGTTT CGGAGGGTGA AGCCCCAAAG
    ATTAAAGCTG AAACCATGAG CAAGAGGAAT GAGATGCGCT ACGGGCCTTC TGCCAGTGCT
    GCACCGCCCA AAGCAGGAAG GTCCTATTTT AAGTCTGTTT TAAAGGAGTG CCTGCTGATG
    CTGGAGTGTT TGGGCATCCC TTGGGTACAA GCAGCTGGGG AAGCCGAGGC CATGTGTGCC
    TACCTGAATG CCCATGGCTA CGTGGATGGA TGCATTACCA ACGATGGCGA TGTCTTCTTG
    TATGGGGCCC AGACAGTTTA TCGGAATTTC ACTATGAATG TGAAGGACCC CCATGTGGAT
    TGCTATGAAG TATCCAAAAT TAAGGCCCAG CTTGGTTTGG ACAGAGAGGA ACTGGTAGGC
    TTGGCCATCC TCCTGGGCTG TGATTATCTC CCGAAAGGGG TGCCTGGAGT CCGAAAAGTA
    CAAGCCCTAA AGCTTATTGA GATGCTAAAT GGGGAGAGTT TGCTGCAAAG GTTCTATCAG
    TGGGAGAAGG ACTGTAACGA ACGGGGCACT AAAGCCCCTA AGAAAAAACC TCACTGTTCT
    GTTTGCTGCC ATCCAGGTTC AGCTAAAGAT CACGAGCGCA ATGGCTGTAA GATGTGCGGG
    AGCGATCGAT TCTGCGAACC ACATGACTAT GATTATCGCT GCCCATGTGA CTGGCATAAA
    GAACAGAGAG AGAAGCAGGA AAATTCTGTG GAGTTTAATA TCAAGAGGAA GTCTCTCCGA
    TGTGAAGGTT TCCCATACCG CCAGGTCATT GATGAATTTC TTATTAATAA GAATAAATTG
    GTGAAAGTGC TTAAATGGGG AAGACCCAGT CTACAGTGTT TTCAGAATTT TACGGCTGAG
    CGAATGGATT GGCCCAGGCA TTACTCGTGT GAGAAGTTGC TTGTGCTGTT GACGTACTAC
    GACATGAATG AGAGAAAGGC AGGCAGAGAG AATGGGGCGC AGCTACAGGC TGTACGCATT
    GTTAAAACAA GAATCAGGAA TGGAAGCCCT TGCTTTGAAA TCGAGTGGGT GAAACCAGAC
    AGTTATGCTT TTGCCGATGA TCATCCCGCT GAGTCTCCTC TGGTGACGAT AGAGGAAGAA
    TCTCTCTTCC GAGCAGCTTA CCCCCATGTT GTCACCATGT TTCACAAGGA GAAGCAAGAA
    GCTGAGGAGA ATAAGCTGAA ATGCAAGAAA AGCAAGCCCA AATCCAAGGC TGCGGATGAT
    CTGGATGATG TTGCGTCTCT GTTGTCCGAA CTGAGCCTGA AACCTGTTTC GGAAAAGCAG
    CCTCCACTAG AGCTACAGGA AGATGGAGGA GACTCGGACA GTGTTCATGA GAAGCCAGCA
    AGGGATTCCA ACTTTCTTAG CTTGTCCAGC GATGCCGATT CTGGAGTGGA AAGAGGGATT
    TTGTTAGATA CCAAAACAGA TTCTGAGTTT TTCACATCAC CCACTGCAGA CAATGAGCCT
    TCCAATCCCT TGTGTTCCTC TCCAGCACCA AGCAATACCC AACATCTTAC TGCTGCTTCT
    CCTAATATTT CTGCCGTGAT TTCAGAACTC CAACTCAGCA GTATCGACTG GGACTCAACC
    TCTTTTAGCA AGTCTCCCCA AGCTGAAGAA CGCACTAAAG GCAGCAAAGA GGTTGGAGAA
    CGGGGGGAGT TTTGTTACAA ACCAACCACC AACAACTTGG AAACTTCAGG AAATAAATCT
    AAGGAGGTTC AGAATGGCAT AGCCAGGCCT GGAACCTCTA ATAAGGTGCA CTTGACTAAT
    TCAAAGCAAT CCAATGTGGA AGACTTTCAT AAGTTACCTT TAAAAGAAAG AGTCCTTCTA
    AAGAATGCTG TTCAGTCTGG TGTCCCTTAT CCAGGAAAAA TGCAAACTAA ACCTGTTCCC
    GTAAAGCCTG TGCCACTATC TCTCCATAAC CAAGGGCTCG GTAATCACAG CTCAAAAAAC
    TCTGTTCCCA CCACTTTGGG TATAGACATT AAGGAAAAAG TACATCAGAA ACTCCCAATG
    AAGTTATCTA GAGATCCGAG TCAACAAGAG AGCAGGCAAC CACCAACTTC AGTCTGTTCC
    AGAGCTAAGC CTCAGAATCA GGGAGCTACT GGGCCAAAAA CATACAATTT TGTTAAGAAT
    GTGAAGGCAG TGCCACTCGT CCCTGTGAAC ACTGGTAATA AGATGGGGAC CCTGGCAGCT
    TCTAAATCCT TAGGGAAGAA GAGCGTCTGT CAAAAAGGGA CCTTATATGA CCAGGAAGAA
    AAAACATACC AACATAAAAT GCAGGCAACA TGCCCAAGCG CAATTGTTCA TCCTCACTCC
    AATGGGGTTG AGGGGGCTAA ATTACATAGA CCACCATATG GGGACAAAGA AGTAAAGCAG
    AACAAAGAGA ACTCCCCTCC AAGAGATAAT CTCATTTGCC ATTCTGGTTT AAACCCTATT
    TTCCTTAGCC CTGACTCTAA GGCAGCCACC AAAAAAGCAA GTGACTGTGC CGCCGAGGAC
    AGCGACGGAG ACGACTCTAT TATATCTGTA GACAGCCCCT TGCCCCTGGC TGAACGTTTA
    AAGCTAAGAT TGTTACAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.