dennd4b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following dennd4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa30656 XM_002942619.3
Latest version!
Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa64205 XM_031890964.1
Latest version!
Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa64205 XM_031890963.1
Latest version!
Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa64206 XM_031890965.1
Latest version!
Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa30656
    Clone ID Related Accession (Same CDS sequence) XM_002942619.3
    Accession Version XM_002942619.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DENN domain-containing protein 4B
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683934.1) and transcript sequence (BC159119.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002942619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2681 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCCCCCT ATGATAACGT CCTGCTGTGC CAGCCAGTAT CCTCTCCTTT GCCCCTCAGC 
    GGAGCCAGTT ACGTGACTCT CCTGCAGAAC CTGGGCCCCG AGACCGCCGT GACCCTACTC
    CTCGCCGTGC TGACCGAACA CAAACTGCTC ATCCACTCCC AGCGGCCCGA TGTGCTGACC
    AGCGTGGGCG AGGCTTTGGT GTCGATCATC TTCCCGCTGC GGTGGCAGTG CCCATACATC
    CCGCTGTGCC CCCTCACCCT CTCCGATGTG CTTTGTGCCC CCGTTCCGTT CATCGTGGGC
    ATCCACTCCA GCTACTTCGA CCTGCACGAC CCGCCCCAGG ATGTTCTGTG TGTGGATCTG
    GACACCAACA CCCTGTTCCA GAGTGAAGAG AAAAAGGTCC CGTCGGTGCG CTCGCTGCCC
    CGCAAACCCT GTAAGGTGCT ACTGGGCACC CTGAGCGACC TGCACAAACA GCTGGACGAG
    ATGTACAACA AACCCATGGA GGAGGCCACA CTGGAGTTCC TGCTGAAAGA CTACGATCTG
    ATCTACGGAC GCCGGACGCA GTTGGAGCTG GACGTACGCG CCGCCTTCCT GAGGTTCATG
    GCATGCTTAT TAAAGGGGTA CCGCTCCTTC CTGCTGCCCA TCACCCAGGC CCCCTCTGAA
    CGGACGCGCG ACTCCAGCAA CCTGTTTAAC GTGGAGGGTT TCCGGAAGTC GCGGGACCGG
    GCGCACCAGA AGTTCTACTC GCAGATGCTG AAGACACAGA TGTTCACACA GTTTATAGAG
    GACTGTTCAT TTGTCAGCGA CCGGCACGCC TGTCTCGAGT TCCTCGACAA TTGCATAGAA
    AAGGTGCAGA TAGATGGGGA GAAGATGGAG GACTTACACC TGGTGGAGTT GGACGAGTCC
    CACCGCAGCG AGCACACTGT GTTCATCATG CCAGCCGAGG AACCTCATGA ACCTGGCAGT
    GGGGAGCTGC CCGCCCAGTA CATGTATGAG AGCTTCCCAG TCCTCAAATG GGAGCTGTTC
    GACCTACCCC AAGATCTACT GATGGCTCCA CCCAACCAAT CCAAGAGCAG CGCCCCGAGC
    AGCCCCGCCC CACGCCGGAC CAAGCAGGAA ATCAAAATGG CTCAGCGCGT GGCCCAGAAG
    TACTCGGCCA TGCCCGACAT GTGGGCCAAG TGCCTGCTGG GCCACTGCTA CGGCCTGTGG
    TTTATTTACC TGCCCACGTT CGTGCGTGCC ACCCCCAGCA AAGTGCGAGC TCTTCAGACC
    GCCTACGATG CCCTGAAGCA GATGGAGAAT AAGAAGGTGG TCCTGCCTGA TGAGGTGTGT
    TACCGGGTCC TGATGCAGCT GTGCGGCCAG TACGGGGAGC CGGTCCTAGC GGTGCGGGTT
    ATGCTGGAAA TGAAAAAAGC TGGCATTGTG CCCAACATCA TCACCTACGG ATACTACAAT
    AAGGCCATAT TGGAAAGCAA ATGGCCCTCG AGCAGTCAGA GCGGGCGCCT ACGCTGGGCC
    AAGCTGCGCA ATGTGGTTCT GGGAGCCGCA CAGTTCCGCC AGCCCCTCAA ACGGAAAAGG
    AGACAGGCCG GACTCAGCCT TTCCCAGACT GAAAGCCATT TGCCAAGGAA CTGCGCCTCT
    GAGCTGCCCC CGCGGCTGAG CCTCCTGCGC CAAGCTACAT GGGCGGGGAG CTCACACAGG
    GAATTCCATG AGAGCTGCGC CGGGAAACTG GTAAAGAGCT GGAGCGTCAG TGGCGCCGGG
    AGTGATGGCG ACATGGTGGA GAGCTCGTTG GCGCACATGA TGGAAGATGC GGAGGGTATG
    AAGATGGGCA GAAGAGGAGA GGGAAGCCCG TCGCTAAGGA GGCCGTGTGG GACCCCCCTG
    GCCCTGGGCC CCTCGGAGAA CGGGAGCCTA TCCAACGTCA GTCTGCAGAC CGATGCCACC
    GGCAGCGACG TGGCTGTAAC TTCAGAAGAC TCGGAAGCCC TGATGACGGA GAGTTCCACG
    GAGGCCTTGC CCTGCCGGCC GGCCAGAGAC CTGGGAGACT CCTCCTCCAC CGGTACCAAC
    GGCAAACGGC ACAGCTTGGC CGGCAAGATA CAGCAGCTTC TCTCCCCGGC CCGGAAGCCT
    GGAAGGGTCA CGTTACTGCG CAAAAAGTCC TCCATGTCTG AAAAACCGTC CCCTCTCAGC
    TTCCCCGGGG ATCAGAGCTG GAGGAGCTAC AAGGAAAGCA TTGGGCGCCC AGAGTCCACG
    GCCTCCGAGA GCTCCGTCTC TCTGGGAAGC GAAGTGGACT TGTCCGACAC CTCCGTCTGC
    AGCTTCACCC TACACAAATC CACCGACCGA CTGACCGACG GGGGGCAGGA AGCGCCGGCG
    GCAGAGGTGA TGCTGTCCGG CTGCTCCCTG TGCCCGGTGT GCCACTCCCT GGTATACGAC
    GAGGACATCA TGGCCGGCTG GACCTCCGAT GACTCCAACC TGAAAACCGT CTGCACCTTC
    TGCGGCAAAT ACTTTGTGCC CCTCCTGAAC GTCAGGGTCA CCGACCTGCA GAGCCGGCAC
    AGTGCTTCCA AGGACTCCTC TGCCTCCCCC CAGCCTTCCA CAAATGGAGA CTCCCCAGTC
    CCTGGGGGGG CGGGGCCAGT GCTGAGCGAC CGCTTCCAGT GTTTGGTGCT CGATGACCAA
    GGGACTGTGC GCCCCCCGTG CAATGGATAC ACCGACACCC AATTACAGCT GCCTGGGCCC
    AGTGCGAGCT CCGAGGTGGT TACGGTGGCC TACCTTTGCC CGCTGGTTCT GCGCAAGGAG
    CTGGAGAGCC TACTGGAGAA TGAAGGTGGA GATGTCCTTT CCCAGCCAGA GCTGGTCAAC
    AGCCACCCAA TCATCTACTG GAACCTTGTG TGGTACTTCA AGCGTATGGC ACTGCCCAGT
    AACCTCCTTC AGCTGGTGTT GGCCTCCAAA CACGTCCGGC AGCAGTCACA GGTGCTGGAG
    GTGCCGCAGG TGAGGGTGCG ACTTCTGTGG GACGTGTTAA TGCAGGACCC GGAGCGGTGG
    CCTCCTCTCT ACGTCCTCTG GAAACTCAAC AGAAACCTCC CCACCCACCT CCATTCCTGG
    AACCACGCCC ACCCCTTTAC CCTGGAAGTT CTGGAGAAGA TCTTGAATTT CGTAGGCCTC
    AACGAGGTTC ATAAAGCAAT TTCCCTGATC ATAGGAATCT TACAGGAGCA GCCCTCCCCA
    CCCCAGAGTA TGCAGAGGGG AATATACCGG GAACTGCTAC TCCTAACACT GGCTGCCCTC
    GGGAAGGATC ACATGGATAT TGGTGCATTT GATAATAAAT ACAAGACGGC CTTCAAGAAG
    CTAAGCAGCT CATTGGGGAA GGAAGAACTG AGAAGAAGAA GGGCCCAGTT CCCCACTCCC
    AAGGCGCTAG ACTGTCGGAG AACCTTTGGG GCCACCCTGG ATTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942665.3
    CDS1239..4646
    Translation

    Target ORF information:

    RefSeq Version XM_002942619.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942619.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCCCCCT ATGATAACGT CCTGCTGTGC CAGCCAGTAT CCTCTCCTTT GCCCCTCAGC 
    GGAGCCAGTT ACGTGACTCT CCTGCAGAAC CTGGGCCCCG AGACCGCCGT GACCCTACTC
    CTCGCCGTGC TGACCGAACA CAAACTGCTC ATCCACTCCC AGCGGCCCGA TGTGCTGACC
    AGCGTGGGCG AGGCTTTGGT GTCGATCATC TTCCCGCTGC GGTGGCAGTG CCCATACATC
    CCGCTGTGCC CCCTCACCCT CTCCGATGTG CTTTGTGCCC CCGTTCCGTT CATCGTGGGC
    ATCCACTCCA GCTACTTCGA CCTGCACGAC CCGCCCCAGG ATGTTCTGTG TGTGGATCTG
    GACACCAACA CCCTGTTCCA GAGTGAAGAG AAAAAGGTCC CGTCGGTGCG CTCGCTGCCC
    CGCAAACCCT GTAAGGTGCT ACTGGGCACC CTGAGCGACC TGCACAAACA GCTGGACGAG
    ATGTACAACA AACCCATGGA GGAGGCCACA CTGGAGTTCC TGCTGAAAGA CTACGATCTG
    ATCTACGGAC GCCGGACGCA GTTGGAGCTG GACGTACGCG CCGCCTTCCT GAGGTTCATG
    GCATGCTTAT TAAAGGGGTA CCGCTCCTTC CTGCTGCCCA TCACCCAGGC CCCCTCTGAA
    CGGACGCGCG ACTCCAGCAA CCTGTTTAAC GTGGAGGGTT TCCGGAAGTC GCGGGACCGG
    GCGCACCAGA AGTTCTACTC GCAGATGCTG AAGACACAGA TGTTCACACA GTTTATAGAG
    GACTGTTCAT TTGTCAGCGA CCGGCACGCC TGTCTCGAGT TCCTCGACAA TTGCATAGAA
    AAGGTGCAGA TAGATGGGGA GAAGATGGAG GACTTACACC TGGTGGAGTT GGACGAGTCC
    CACCGCAGCG AGCACACTGT GTTCATCATG CCAGCCGAGG AACCTCATGA ACCTGGCAGT
    GGGGAGCTGC CCGCCCAGTA CATGTATGAG AGCTTCCCAG TCCTCAAATG GGAGCTGTTC
    GACCTACCCC AAGATCTACT GATGGCTCCA CCCAACCAAT CCAAGAGCAG CGCCCCGAGC
    AGCCCCGCCC CACGCCGGAC CAAGCAGGAA ATCAAAATGG CTCAGCGCGT GGCCCAGAAG
    TACTCGGCCA TGCCCGACAT GTGGGCCAAG TGCCTGCTGG GCCACTGCTA CGGCCTGTGG
    TTTATTTACC TGCCCACGTT CGTGCGTGCC ACCCCCAGCA AAGTGCGAGC TCTTCAGACC
    GCCTACGATG CCCTGAAGCA GATGGAGAAT AAGAAGGTGG TCCTGCCTGA TGAGGTGTGT
    TACCGGGTCC TGATGCAGCT GTGCGGCCAG TACGGGGAGC CGGTCCTAGC GGTGCGGGTT
    ATGCTGGAAA TGAAAAAAGC TGGCATTGTG CCCAACATCA TCACCTACGG ATACTACAAT
    AAGGCCATAT TGGAAAGCAA ATGGCCCTCG AGCAGTCAGA GCGGGCGCCT ACGCTGGGCC
    AAGCTGCGCA ATGTGGTTCT GGGAGCCGCA CAGTTCCGCC AGCCCCTCAA ACGGAAAAGG
    AGACAGGCCG GACTCAGCCT TTCCCAGACT GAAAGCCATT TGCCAAGGAA CTGCGCCTCT
    GAGCTGCCCC CGCGGCTGAG CCTCCTGCGC CAAGCTACAT GGGCGGGGAG CTCACACAGG
    GAATTCCATG AGAGCTGCGC CGGGAAACTG GTAAAGAGCT GGAGCGTCAG TGGCGCCGGG
    AGTGATGGCG ACATGGTGGA GAGCTCGTTG GCGCACATGA TGGAAGATGC GGAGGGTATG
    AAGATGGGCA GAAGAGGAGA GGGAAGCCCG TCGCTAAGGA GGCCGTGTGG GACCCCCCTG
    GCCCTGGGCC CCTCGGAGAA CGGGAGCCTA TCCAACGTCA GTCTGCAGAC CGATGCCACC
    GGCAGCGACG TGGCTGTAAC TTCAGAAGAC TCGGAAGCCC TGATGACGGA GAGTTCCACG
    GAGGCCTTGC CCTGCCGGCC GGCCAGAGAC CTGGGAGACT CCTCCTCCAC CGGTACCAAC
    GGCAAACGGC ACAGCTTGGC CGGCAAGATA CAGCAGCTTC TCTCCCCGGC CCGGAAGCCT
    GGAAGGGTCA CGTTACTGCG CAAAAAGTCC TCCATGTCTG AAAAACCGTC CCCTCTCAGC
    TTCCCCGGGG ATCAGAGCTG GAGGAGCTAC AAGGAAAGCA TTGGGCGCCC AGAGTCCACG
    GCCTCCGAGA GCTCCGTCTC TCTGGGAAGC GAAGTGGACT TGTCCGACAC CTCCGTCTGC
    AGCTTCACCC TACACAAATC CACCGACCGA CTGACCGACG GGGGGCAGGA AGCGCCGGCG
    GCAGAGGTGA TGCTGTCCGG CTGCTCCCTG TGCCCGGTGT GCCACTCCCT GGTATACGAC
    GAGGACATCA TGGCCGGCTG GACCTCCGAT GACTCCAACC TGAAAACCGT CTGCACCTTC
    TGCGGCAAAT ACTTTGTGCC CCTCCTGAAC GTCAGGGTCA CCGACCTGCA GAGCCGGCAC
    AGTGCTTCCA AGGACTCCTC TGCCTCCCCC CAGCCTTCCA CAAATGGAGA CTCCCCAGTC
    CCTGGGGGGG CGGGGCCAGT GCTGAGCGAC CGCTTCCAGT GTTTGGTGCT CGATGACCAA
    GGGACTGTGC GCCCCCCGTG CAATGGATAC ACCGACACCC AATTACAGCT GCCTGGGCCC
    AGTGCGAGCT CCGAGGTGGT TACGGTGGCC TACCTTTGCC CGCTGGTTCT GCGCAAGGAG
    CTGGAGAGCC TACTGGAGAA TGAAGGTGGA GATGTCCTTT CCCAGCCAGA GCTGGTCAAC
    AGCCACCCAA TCATCTACTG GAACCTTGTG TGGTACTTCA AGCGTATGGC ACTGCCCAGT
    AACCTCCTTC AGCTGGTGTT GGCCTCCAAA CACGTCCGGC AGCAGTCACA GGTGCTGGAG
    GTGCCGCAGG TGAGGGTGCG ACTTCTGTGG GACGTGTTAA TGCAGGACCC GGAGCGGTGG
    CCTCCTCTCT ACGTCCTCTG GAAACTCAAC AGAAACCTCC CCACCCACCT CCATTCCTGG
    AACCACGCCC ACCCCTTTAC CCTGGAAGTT CTGGAGAAGA TCTTGAATTT CGTAGGCCTC
    AACGAGGTTC ATAAAGCAAT TTCCCTGATC ATAGGAATCT TACAGGAGCA GCCCTCCCCA
    CCCCAGAGTA TGCAGAGGGG AATATACCGG GAACTGCTAC TCCTAACACT GGCTGCCCTC
    GGGAAGGATC ACATGGATAT TGGTGCATTT GATAATAAAT ACAAGACGGC CTTCAAGAAG
    CTAAGCAGCT CATTGGGGAA GGAAGAACTG AGAAGAAGAA GGGCCCAGTT CCCCACTCCC
    AAGGCGCTAG ACTGTCGGAG AACCTTTGGG GCCACCCTGG ATTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64205
    Clone ID Related Accession (Same CDS sequence) XM_031890964.1 , XM_031890963.1
    Accession Version XM_031890964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DENN domain-containing protein 4B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAGAGG GACTGCCCAA ACTGGTGGAT TACTTTGTAG TGGCCGGCCT GACCCCGGCG 
    TCTCGGCCCT TGGAGGAAGA GAACCGCCAG CGCTCTATTC GCACCACAGA GCTGGTCACT
    GACGTGGCGG TCATTGTCAA GGCGCAGGGG GAGGAGGTTC CGCAGGGGTT TACCTGTATA
    GAGACCACCC CCGGGGGGCA CTCGGCCGAT CTGAATTCCG GCTTACTCAC CAACCAGCAG
    ATGTACCTGT GCTACCGCAG GGGCAGAGAC AAGCCCCCTA TCACCGAGTT AGGAGTGCAC
    TATGACGGCA AAGAACCTCT CCGCCCGGGC TTCCAGGTTA TCGACACCAC TCCGTACAGT
    CACTCTGCCA ACCTGAGCTC GGGGGGCCCC GGGAACCAGC GAGCCTTCCT GATGTTCAAA
    CGGGCCCCCG AATCCATGGG GCTGAATAGT CTGGGGGTGA TGGATATCTG TATCATTAAT
    CCCAGCAAGG GGGAAAGCAC CCCCAACACC TTTTGCCGAG TGGATAGGAA CCTCAATACA
    AGTATGTTCG GGCCGGCTCT GTTCCTCTGT TACAAGAAGG GCACGGCTAA GACCCACAGC
    CTTGTGTATG AGGCAGGGGT GCTCAGCCGC TTCCCTAGTG CCGACAGTGA AACGTTTCCC
    TTGCCCGAGA TGGTGGCGAC CTTCTGTCTG CCAATGGGTG CCACCATTGA GAGCTGGCCA
    ATCAACACTA AGTATCACAT GCCCGTCTTC TCCACCTTTG TGCTGACTGG GGCTTCTGGG
    GAGAAGGTAT ATGGCGCAGC CATCCAGTTC CATGAGCAAT ACCCCCGGGG GTGCCTGAGC
    GAGAAGCAGA ACCAATCCCT GGGGCTTCTG AGTGTGGTGG ACAAGCGCCC CGTGACCAAC
    AAATCTGTCC AGACCAAGAA GAGCATCTGT GTCCTCTCTC ATTGGCCCTT CTTCGACGTC
    TTCCAGAAGT TCCTCACTTT CATTTATCGA TACTCCATCT CCGGCCCCCA CGTCCTTCCC
    ATTGAGAAAC ATATCTCCAA CTTCATGTTC AATGTCCCCT TCCCGTCCCC ACAGCGGCCG
    CGCATTTTGG TTCAGATGTC CCCCTATGAT AACGTCCTGC TGTGCCAGCC AGTATCCTCT
    CCTTTGCCCC TCAGCGGAGC CAGTTACGTG ACTCTCCTGC AGAACCTGGG CCCCGAGACC
    GCCGTGACCC TACTCCTCGC CGTGCTGACC GAACACAAAC TGCTCATCCA CTCCCAGCGG
    CCCGATGTGC TGACCAGCGT GGGCGAGGCT TTGGTGTCGA TCATCTTCCC GCTGCGGTGG
    CAGTGCCCAT ACATCCCGCT GTGCCCCCTC ACCCTCTCCG ATGTGCTTTG TGCCCCTGTT
    CCGTTCATCG TGGGCATCCA CTCCAGCTAC TTCGACCTGC ACGACCCGCC CCAGGATGTT
    CTGTGTGTGG ATCTGGACAC CAACACCCTG TTCCAGAGTG AAGAGAAAAA GGTCCCATCG
    GTGCGCTCGC TGCCCCGCAA ACCCTGTAAG GTGCTACTGG GCACCCTGAG CGACCTGCAC
    AAACAGCTGG ACGAGATGTA CAACAAACCC ATGGAGGAGG CCACACTGGA GTTCCTGCTG
    AAAGACTACG ATCTGATCTA CGGACGCCGG ACGCAGTTGG AGCTGGACGT ACGCGCCGCC
    TTCCTGAGGT TCATGGCATG CTTATTAAAG GGGTACCGCT CCTTCCTGCT GCCCATCACC
    CAGGCCCCCT CTGAACGGAC GCGCGACTCC AGCAACCTGT TTAACGTGGA GGGTTTCCGG
    AAGTCGCGGG ACCGGGCGCA CCAGAAGTTC TACTCGCAGA TGCTGAAGAC ACAGATGTTC
    ACACAGTTTA TAGAGGACTG CTCCTTTGTT AGCGACCGGC ACGCCTGTCT CGAGTTCCTC
    GACAATTGCA TAGAAAAGGT GCAGATAGAT GGGGAGAAGA TGGAGGACTT ACACCTGGTG
    GAGTTGGACG AGTCCCACCG CAGCGAGCAC ACTGTGTTCA TCATGCCAGC CGAGGAACCT
    CATGAACCTG GCAGTGGGGA GCTGCCCGCC CAGTACATGT ATGAGAGCTT CCCAGTCCTC
    AAATGGGAGC TGTTCGACCT ACCCCAAGAT CTACTGATGG CTCCACCCAA CCAATCCAAG
    AGCAGCGCCC CGAGCAGCCC CGCCCCACGC CGGACCAAGC AGGAAATAAA AATGGCTCAG
    CGCGTGGCCC AGAAGTACTC AGCCATGCCC GACATGTGGG CCAAGTGCCT GCTGGGCCAC
    TGCTACGGCC TGTGGTTTAT TTACCTGCCC ACGTTCGTGC GTGCCACCCC CAGCAAAGTG
    CGAGCTCTTC AGACCGCCTA CGATGCCCTG AAGCAGATGG AGAATAAGAA GGTGGTCCTG
    CCTGATGAGG TGTGTTACCG GGTCCTGATG CAGCTGTGCG GCCAGTACGG GGAGCCGGTC
    CTAGCGGTGC GGGTTATGCT GGAAATGAAA AAAGCTGGCA TTGTGCCCAA CATCATCACC
    TACGGATACT ACAATAAGGC CATATTGGAA AGCAAATGGC CCTCGAGCAG TCAGAGCGGG
    CGCCTACGCT GGGCCAAGCT GCGCAATGTG GTTCTGGGAG CCGCACAGTT CCGCCAGCCC
    CTCAAACGGA AAAGGAGACA GGCCGGACTC AGCCTTTCCC AGACTGAAAG CCATTTGCCA
    AGGAACTGCG CCTCTGAGCT GCCCCCGCGG CTGAGCCTCC TGCGCCAAGC TACATGGGCG
    GGGAGCTCAC ACAGGGAATT CCATGAGAGC TGCGCCGGGA AACTGGTAAA GAGCTGGAGC
    GTCAGTGGCG CCGGGAGTGA TGGCGACATG GTGGAGAGCT CGTTGGCGCA CATGATGGAA
    GATGCGGAGG GTATGAAGAT GGGCAGAAGA GGAGAGGGAA GCCCGTCGCT AAGGAGGCCG
    TGTGGGACCC CCCTGGCCCT GGGCCCCTCG GAGAACGGGA GCCTATCCAA CGTCAGTCTG
    CAGACCGATG CCACCGGCAG CGACGTGGCT GTAACTTCAG AAGACTCGGA AGCCCTGATG
    ACGGAGAGTT CCACGGAGGC CTTGCCCTGC CGGCCGGCCA GAGACCTGGG AGACTCCTCC
    TCCACCGGTA CCAACGGCAA ACGGCACAGC TTGGCCGGCA AGATACAGCA GCTTCTCTCC
    CCGGCCCGGA AGCCTGGAAG GGTCACGTTA CTGCGCAAAA AGTCCTCCAT GTCTGAAAAA
    CCGTCCCCTC TCAGCTTCCC CGGGGATCAG AGCTGGAGGA GCTACAAGGA AAGCATTGGG
    CGCCCAGAGT CCACGGCCTC CGAGGTGAGT AAGAGCTCCG TCTCTCTGGG AAGCGAAGTG
    GACTTGTCCG ACACCTCCGT CTGCAGCTTC ACCCTACACA AATCCACCGA CCGACTGACC
    GACGGGGGGC AGGAAGCGCC GGCGGCAGAG GTGATGCTGT CCGGCTGCTC CCTGTGCCCG
    GTGTGCCACT CCCTGGTATA CGACGAGGAC ATCATGGCCG GCTGGACCTC CGATGACTCC
    AACCTGAAAA CCGTCTGCAC CTTCTGCGGC AAATACTTTG TGCCCCTCCT GAACGTCAGG
    GTCACCGACC TGCAGAGCCG GCACAGTGCT TCCAAGGACT CCTCTGCCTC CCCCCAGCCT
    TCCACAAATG GAGACTCCCC AGTCCCTGGG GGGGCGGGGC CAGTGCTGAG CGACCGCTTC
    CAGTGTTTGG TGCTCGATGA CCAAGGGACT GTGCGCCCCC CGTGCAATGG ATACACCGAC
    ACCCAATTAC AGCTGCCTGG GCCCAGTGCG AGCTCCGAGG TGGTTACGGT GGCCTACCTT
    TGCCCGCTGG TTCTGCGCAA GGAGCTGGAG AGCCTACTGG AGAATGAAGG TGGAGATGTC
    CTTTCCCAGC CAGAGCTGGT CAACAGCCAC CCAATCATCT ACTGGAACCT TGTGTGGTAC
    TTCAAGCGTA TGGCACTGCC CAGTAACCTC CTTCAGCTGG TGTTGGCCTC CAAACACGTC
    CGGCAGCAGT CACAGGTGCT GGAGGTGCCG CAGGTGAGGG TGCGACTTCT GTGGGACGTG
    TTAATGCAGG ACCCGGAGCG GTGGCCTCCT CTCTACGTCC TCTGGAAACT CAACAGAAAC
    CTCCCCACCC ACCTCCATTC CTGGAACCAC GCCCACCCCT TTACCCTGGA AGTTCTGGAG
    AAGATCTTGA ATTTCGTAGG CCTCAACGAG GTTCATAAAG CAATTTCCCT GATCATAGGA
    ATCTTACAGG AGCAGCCCTC CCCACCCCAG AGTATGCAGA GGGGAATATA CCGGGAACTG
    CTACTCCTAA CACTGGCTGC CCTCGGGAAG GATCACATGG ATATTGGTGC ATTTGATAAT
    AAATACAAGA CGGCCTTCAA GAAGCTAAGC AGCTCATTGG GGAAGGAAGA ACTGAGAAGA
    AGAAGGGCCC AGTTCCCCAC TCCCAAGGCG CTAGACTGTC GGAGAACCTT TGGGGCCACC
    CTGGATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746824.1
    CDS358..4869
    Translation

    Target ORF information:

    RefSeq Version XM_031890964.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAGAGG GACTGCCCAA ACTGGTGGAT TACTTTGTAG TGGCCGGCCT GACCCCGGCG 
    TCTCGGCCCT TGGAGGAAGA GAACCGCCAG CGCTCTATTC GCACCACAGA GCTGGTCACT
    GACGTGGCGG TCATTGTCAA GGCGCAGGGG GAGGAGGTTC CGCAGGGGTT TACCTGTATA
    GAGACCACCC CCGGGGGGCA CTCGGCCGAT CTGAATTCCG GCTTACTCAC CAACCAGCAG
    ATGTACCTGT GCTACCGCAG GGGCAGAGAC AAGCCCCCTA TCACCGAGTT AGGAGTGCAC
    TATGACGGCA AAGAACCTCT CCGCCCGGGC TTCCAGGTTA TCGACACCAC TCCGTACAGT
    CACTCTGCCA ACCTGAGCTC GGGGGGCCCC GGGAACCAGC GAGCCTTCCT GATGTTCAAA
    CGGGCCCCCG AATCCATGGG GCTGAATAGT CTGGGGGTGA TGGATATCTG TATCATTAAT
    CCCAGCAAGG GGGAAAGCAC CCCCAACACC TTTTGCCGAG TGGATAGGAA CCTCAATACA
    AGTATGTTCG GGCCGGCTCT GTTCCTCTGT TACAAGAAGG GCACGGCTAA GACCCACAGC
    CTTGTGTATG AGGCAGGGGT GCTCAGCCGC TTCCCTAGTG CCGACAGTGA AACGTTTCCC
    TTGCCCGAGA TGGTGGCGAC CTTCTGTCTG CCAATGGGTG CCACCATTGA GAGCTGGCCA
    ATCAACACTA AGTATCACAT GCCCGTCTTC TCCACCTTTG TGCTGACTGG GGCTTCTGGG
    GAGAAGGTAT ATGGCGCAGC CATCCAGTTC CATGAGCAAT ACCCCCGGGG GTGCCTGAGC
    GAGAAGCAGA ACCAATCCCT GGGGCTTCTG AGTGTGGTGG ACAAGCGCCC CGTGACCAAC
    AAATCTGTCC AGACCAAGAA GAGCATCTGT GTCCTCTCTC ATTGGCCCTT CTTCGACGTC
    TTCCAGAAGT TCCTCACTTT CATTTATCGA TACTCCATCT CCGGCCCCCA CGTCCTTCCC
    ATTGAGAAAC ATATCTCCAA CTTCATGTTC AATGTCCCCT TCCCGTCCCC ACAGCGGCCG
    CGCATTTTGG TTCAGATGTC CCCCTATGAT AACGTCCTGC TGTGCCAGCC AGTATCCTCT
    CCTTTGCCCC TCAGCGGAGC CAGTTACGTG ACTCTCCTGC AGAACCTGGG CCCCGAGACC
    GCCGTGACCC TACTCCTCGC CGTGCTGACC GAACACAAAC TGCTCATCCA CTCCCAGCGG
    CCCGATGTGC TGACCAGCGT GGGCGAGGCT TTGGTGTCGA TCATCTTCCC GCTGCGGTGG
    CAGTGCCCAT ACATCCCGCT GTGCCCCCTC ACCCTCTCCG ATGTGCTTTG TGCCCCTGTT
    CCGTTCATCG TGGGCATCCA CTCCAGCTAC TTCGACCTGC ACGACCCGCC CCAGGATGTT
    CTGTGTGTGG ATCTGGACAC CAACACCCTG TTCCAGAGTG AAGAGAAAAA GGTCCCATCG
    GTGCGCTCGC TGCCCCGCAA ACCCTGTAAG GTGCTACTGG GCACCCTGAG CGACCTGCAC
    AAACAGCTGG ACGAGATGTA CAACAAACCC ATGGAGGAGG CCACACTGGA GTTCCTGCTG
    AAAGACTACG ATCTGATCTA CGGACGCCGG ACGCAGTTGG AGCTGGACGT ACGCGCCGCC
    TTCCTGAGGT TCATGGCATG CTTATTAAAG GGGTACCGCT CCTTCCTGCT GCCCATCACC
    CAGGCCCCCT CTGAACGGAC GCGCGACTCC AGCAACCTGT TTAACGTGGA GGGTTTCCGG
    AAGTCGCGGG ACCGGGCGCA CCAGAAGTTC TACTCGCAGA TGCTGAAGAC ACAGATGTTC
    ACACAGTTTA TAGAGGACTG CTCCTTTGTT AGCGACCGGC ACGCCTGTCT CGAGTTCCTC
    GACAATTGCA TAGAAAAGGT GCAGATAGAT GGGGAGAAGA TGGAGGACTT ACACCTGGTG
    GAGTTGGACG AGTCCCACCG CAGCGAGCAC ACTGTGTTCA TCATGCCAGC CGAGGAACCT
    CATGAACCTG GCAGTGGGGA GCTGCCCGCC CAGTACATGT ATGAGAGCTT CCCAGTCCTC
    AAATGGGAGC TGTTCGACCT ACCCCAAGAT CTACTGATGG CTCCACCCAA CCAATCCAAG
    AGCAGCGCCC CGAGCAGCCC CGCCCCACGC CGGACCAAGC AGGAAATAAA AATGGCTCAG
    CGCGTGGCCC AGAAGTACTC AGCCATGCCC GACATGTGGG CCAAGTGCCT GCTGGGCCAC
    TGCTACGGCC TGTGGTTTAT TTACCTGCCC ACGTTCGTGC GTGCCACCCC CAGCAAAGTG
    CGAGCTCTTC AGACCGCCTA CGATGCCCTG AAGCAGATGG AGAATAAGAA GGTGGTCCTG
    CCTGATGAGG TGTGTTACCG GGTCCTGATG CAGCTGTGCG GCCAGTACGG GGAGCCGGTC
    CTAGCGGTGC GGGTTATGCT GGAAATGAAA AAAGCTGGCA TTGTGCCCAA CATCATCACC
    TACGGATACT ACAATAAGGC CATATTGGAA AGCAAATGGC CCTCGAGCAG TCAGAGCGGG
    CGCCTACGCT GGGCCAAGCT GCGCAATGTG GTTCTGGGAG CCGCACAGTT CCGCCAGCCC
    CTCAAACGGA AAAGGAGACA GGCCGGACTC AGCCTTTCCC AGACTGAAAG CCATTTGCCA
    AGGAACTGCG CCTCTGAGCT GCCCCCGCGG CTGAGCCTCC TGCGCCAAGC TACATGGGCG
    GGGAGCTCAC ACAGGGAATT CCATGAGAGC TGCGCCGGGA AACTGGTAAA GAGCTGGAGC
    GTCAGTGGCG CCGGGAGTGA TGGCGACATG GTGGAGAGCT CGTTGGCGCA CATGATGGAA
    GATGCGGAGG GTATGAAGAT GGGCAGAAGA GGAGAGGGAA GCCCGTCGCT AAGGAGGCCG
    TGTGGGACCC CCCTGGCCCT GGGCCCCTCG GAGAACGGGA GCCTATCCAA CGTCAGTCTG
    CAGACCGATG CCACCGGCAG CGACGTGGCT GTAACTTCAG AAGACTCGGA AGCCCTGATG
    ACGGAGAGTT CCACGGAGGC CTTGCCCTGC CGGCCGGCCA GAGACCTGGG AGACTCCTCC
    TCCACCGGTA CCAACGGCAA ACGGCACAGC TTGGCCGGCA AGATACAGCA GCTTCTCTCC
    CCGGCCCGGA AGCCTGGAAG GGTCACGTTA CTGCGCAAAA AGTCCTCCAT GTCTGAAAAA
    CCGTCCCCTC TCAGCTTCCC CGGGGATCAG AGCTGGAGGA GCTACAAGGA AAGCATTGGG
    CGCCCAGAGT CCACGGCCTC CGAGGTGAGT AAGAGCTCCG TCTCTCTGGG AAGCGAAGTG
    GACTTGTCCG ACACCTCCGT CTGCAGCTTC ACCCTACACA AATCCACCGA CCGACTGACC
    GACGGGGGGC AGGAAGCGCC GGCGGCAGAG GTGATGCTGT CCGGCTGCTC CCTGTGCCCG
    GTGTGCCACT CCCTGGTATA CGACGAGGAC ATCATGGCCG GCTGGACCTC CGATGACTCC
    AACCTGAAAA CCGTCTGCAC CTTCTGCGGC AAATACTTTG TGCCCCTCCT GAACGTCAGG
    GTCACCGACC TGCAGAGCCG GCACAGTGCT TCCAAGGACT CCTCTGCCTC CCCCCAGCCT
    TCCACAAATG GAGACTCCCC AGTCCCTGGG GGGGCGGGGC CAGTGCTGAG CGACCGCTTC
    CAGTGTTTGG TGCTCGATGA CCAAGGGACT GTGCGCCCCC CGTGCAATGG ATACACCGAC
    ACCCAATTAC AGCTGCCTGG GCCCAGTGCG AGCTCCGAGG TGGTTACGGT GGCCTACCTT
    TGCCCGCTGG TTCTGCGCAA GGAGCTGGAG AGCCTACTGG AGAATGAAGG TGGAGATGTC
    CTTTCCCAGC CAGAGCTGGT CAACAGCCAC CCAATCATCT ACTGGAACCT TGTGTGGTAC
    TTCAAGCGTA TGGCACTGCC CAGTAACCTC CTTCAGCTGG TGTTGGCCTC CAAACACGTC
    CGGCAGCAGT CACAGGTGCT GGAGGTGCCG CAGGTGAGGG TGCGACTTCT GTGGGACGTG
    TTAATGCAGG ACCCGGAGCG GTGGCCTCCT CTCTACGTCC TCTGGAAACT CAACAGAAAC
    CTCCCCACCC ACCTCCATTC CTGGAACCAC GCCCACCCCT TTACCCTGGA AGTTCTGGAG
    AAGATCTTGA ATTTCGTAGG CCTCAACGAG GTTCATAAAG CAATTTCCCT GATCATAGGA
    ATCTTACAGG AGCAGCCCTC CCCACCCCAG AGTATGCAGA GGGGAATATA CCGGGAACTG
    CTACTCCTAA CACTGGCTGC CCTCGGGAAG GATCACATGG ATATTGGTGC ATTTGATAAT
    AAATACAAGA CGGCCTTCAA GAAGCTAAGC AGCTCATTGG GGAAGGAAGA ACTGAGAAGA
    AGAAGGGCCC AGTTCCCCAC TCCCAAGGCG CTAGACTGTC GGAGAACCTT TGGGGCCACC
    CTGGATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64205
    Clone ID Related Accession (Same CDS sequence) XM_031890964.1 , XM_031890963.1
    Accession Version XM_031890963.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DENN domain-containing protein 4B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAGAGG GACTGCCCAA ACTGGTGGAT TACTTTGTAG TGGCCGGCCT GACCCCGGCG 
    TCTCGGCCCT TGGAGGAAGA GAACCGCCAG CGCTCTATTC GCACCACAGA GCTGGTCACT
    GACGTGGCGG TCATTGTCAA GGCGCAGGGG GAGGAGGTTC CGCAGGGGTT TACCTGTATA
    GAGACCACCC CCGGGGGGCA CTCGGCCGAT CTGAATTCCG GCTTACTCAC CAACCAGCAG
    ATGTACCTGT GCTACCGCAG GGGCAGAGAC AAGCCCCCTA TCACCGAGTT AGGAGTGCAC
    TATGACGGCA AAGAACCTCT CCGCCCGGGC TTCCAGGTTA TCGACACCAC TCCGTACAGT
    CACTCTGCCA ACCTGAGCTC GGGGGGCCCC GGGAACCAGC GAGCCTTCCT GATGTTCAAA
    CGGGCCCCCG AATCCATGGG GCTGAATAGT CTGGGGGTGA TGGATATCTG TATCATTAAT
    CCCAGCAAGG GGGAAAGCAC CCCCAACACC TTTTGCCGAG TGGATAGGAA CCTCAATACA
    AGTATGTTCG GGCCGGCTCT GTTCCTCTGT TACAAGAAGG GCACGGCTAA GACCCACAGC
    CTTGTGTATG AGGCAGGGGT GCTCAGCCGC TTCCCTAGTG CCGACAGTGA AACGTTTCCC
    TTGCCCGAGA TGGTGGCGAC CTTCTGTCTG CCAATGGGTG CCACCATTGA GAGCTGGCCA
    ATCAACACTA AGTATCACAT GCCCGTCTTC TCCACCTTTG TGCTGACTGG GGCTTCTGGG
    GAGAAGGTAT ATGGCGCAGC CATCCAGTTC CATGAGCAAT ACCCCCGGGG GTGCCTGAGC
    GAGAAGCAGA ACCAATCCCT GGGGCTTCTG AGTGTGGTGG ACAAGCGCCC CGTGACCAAC
    AAATCTGTCC AGACCAAGAA GAGCATCTGT GTCCTCTCTC ATTGGCCCTT CTTCGACGTC
    TTCCAGAAGT TCCTCACTTT CATTTATCGA TACTCCATCT CCGGCCCCCA CGTCCTTCCC
    ATTGAGAAAC ATATCTCCAA CTTCATGTTC AATGTCCCCT TCCCGTCCCC ACAGCGGCCG
    CGCATTTTGG TTCAGATGTC CCCCTATGAT AACGTCCTGC TGTGCCAGCC AGTATCCTCT
    CCTTTGCCCC TCAGCGGAGC CAGTTACGTG ACTCTCCTGC AGAACCTGGG CCCCGAGACC
    GCCGTGACCC TACTCCTCGC CGTGCTGACC GAACACAAAC TGCTCATCCA CTCCCAGCGG
    CCCGATGTGC TGACCAGCGT GGGCGAGGCT TTGGTGTCGA TCATCTTCCC GCTGCGGTGG
    CAGTGCCCAT ACATCCCGCT GTGCCCCCTC ACCCTCTCCG ATGTGCTTTG TGCCCCTGTT
    CCGTTCATCG TGGGCATCCA CTCCAGCTAC TTCGACCTGC ACGACCCGCC CCAGGATGTT
    CTGTGTGTGG ATCTGGACAC CAACACCCTG TTCCAGAGTG AAGAGAAAAA GGTCCCATCG
    GTGCGCTCGC TGCCCCGCAA ACCCTGTAAG GTGCTACTGG GCACCCTGAG CGACCTGCAC
    AAACAGCTGG ACGAGATGTA CAACAAACCC ATGGAGGAGG CCACACTGGA GTTCCTGCTG
    AAAGACTACG ATCTGATCTA CGGACGCCGG ACGCAGTTGG AGCTGGACGT ACGCGCCGCC
    TTCCTGAGGT TCATGGCATG CTTATTAAAG GGGTACCGCT CCTTCCTGCT GCCCATCACC
    CAGGCCCCCT CTGAACGGAC GCGCGACTCC AGCAACCTGT TTAACGTGGA GGGTTTCCGG
    AAGTCGCGGG ACCGGGCGCA CCAGAAGTTC TACTCGCAGA TGCTGAAGAC ACAGATGTTC
    ACACAGTTTA TAGAGGACTG CTCCTTTGTT AGCGACCGGC ACGCCTGTCT CGAGTTCCTC
    GACAATTGCA TAGAAAAGGT GCAGATAGAT GGGGAGAAGA TGGAGGACTT ACACCTGGTG
    GAGTTGGACG AGTCCCACCG CAGCGAGCAC ACTGTGTTCA TCATGCCAGC CGAGGAACCT
    CATGAACCTG GCAGTGGGGA GCTGCCCGCC CAGTACATGT ATGAGAGCTT CCCAGTCCTC
    AAATGGGAGC TGTTCGACCT ACCCCAAGAT CTACTGATGG CTCCACCCAA CCAATCCAAG
    AGCAGCGCCC CGAGCAGCCC CGCCCCACGC CGGACCAAGC AGGAAATAAA AATGGCTCAG
    CGCGTGGCCC AGAAGTACTC AGCCATGCCC GACATGTGGG CCAAGTGCCT GCTGGGCCAC
    TGCTACGGCC TGTGGTTTAT TTACCTGCCC ACGTTCGTGC GTGCCACCCC CAGCAAAGTG
    CGAGCTCTTC AGACCGCCTA CGATGCCCTG AAGCAGATGG AGAATAAGAA GGTGGTCCTG
    CCTGATGAGG TGTGTTACCG GGTCCTGATG CAGCTGTGCG GCCAGTACGG GGAGCCGGTC
    CTAGCGGTGC GGGTTATGCT GGAAATGAAA AAAGCTGGCA TTGTGCCCAA CATCATCACC
    TACGGATACT ACAATAAGGC CATATTGGAA AGCAAATGGC CCTCGAGCAG TCAGAGCGGG
    CGCCTACGCT GGGCCAAGCT GCGCAATGTG GTTCTGGGAG CCGCACAGTT CCGCCAGCCC
    CTCAAACGGA AAAGGAGACA GGCCGGACTC AGCCTTTCCC AGACTGAAAG CCATTTGCCA
    AGGAACTGCG CCTCTGAGCT GCCCCCGCGG CTGAGCCTCC TGCGCCAAGC TACATGGGCG
    GGGAGCTCAC ACAGGGAATT CCATGAGAGC TGCGCCGGGA AACTGGTAAA GAGCTGGAGC
    GTCAGTGGCG CCGGGAGTGA TGGCGACATG GTGGAGAGCT CGTTGGCGCA CATGATGGAA
    GATGCGGAGG GTATGAAGAT GGGCAGAAGA GGAGAGGGAA GCCCGTCGCT AAGGAGGCCG
    TGTGGGACCC CCCTGGCCCT GGGCCCCTCG GAGAACGGGA GCCTATCCAA CGTCAGTCTG
    CAGACCGATG CCACCGGCAG CGACGTGGCT GTAACTTCAG AAGACTCGGA AGCCCTGATG
    ACGGAGAGTT CCACGGAGGC CTTGCCCTGC CGGCCGGCCA GAGACCTGGG AGACTCCTCC
    TCCACCGGTA CCAACGGCAA ACGGCACAGC TTGGCCGGCA AGATACAGCA GCTTCTCTCC
    CCGGCCCGGA AGCCTGGAAG GGTCACGTTA CTGCGCAAAA AGTCCTCCAT GTCTGAAAAA
    CCGTCCCCTC TCAGCTTCCC CGGGGATCAG AGCTGGAGGA GCTACAAGGA AAGCATTGGG
    CGCCCAGAGT CCACGGCCTC CGAGGTGAGT AAGAGCTCCG TCTCTCTGGG AAGCGAAGTG
    GACTTGTCCG ACACCTCCGT CTGCAGCTTC ACCCTACACA AATCCACCGA CCGACTGACC
    GACGGGGGGC AGGAAGCGCC GGCGGCAGAG GTGATGCTGT CCGGCTGCTC CCTGTGCCCG
    GTGTGCCACT CCCTGGTATA CGACGAGGAC ATCATGGCCG GCTGGACCTC CGATGACTCC
    AACCTGAAAA CCGTCTGCAC CTTCTGCGGC AAATACTTTG TGCCCCTCCT GAACGTCAGG
    GTCACCGACC TGCAGAGCCG GCACAGTGCT TCCAAGGACT CCTCTGCCTC CCCCCAGCCT
    TCCACAAATG GAGACTCCCC AGTCCCTGGG GGGGCGGGGC CAGTGCTGAG CGACCGCTTC
    CAGTGTTTGG TGCTCGATGA CCAAGGGACT GTGCGCCCCC CGTGCAATGG ATACACCGAC
    ACCCAATTAC AGCTGCCTGG GCCCAGTGCG AGCTCCGAGG TGGTTACGGT GGCCTACCTT
    TGCCCGCTGG TTCTGCGCAA GGAGCTGGAG AGCCTACTGG AGAATGAAGG TGGAGATGTC
    CTTTCCCAGC CAGAGCTGGT CAACAGCCAC CCAATCATCT ACTGGAACCT TGTGTGGTAC
    TTCAAGCGTA TGGCACTGCC CAGTAACCTC CTTCAGCTGG TGTTGGCCTC CAAACACGTC
    CGGCAGCAGT CACAGGTGCT GGAGGTGCCG CAGGTGAGGG TGCGACTTCT GTGGGACGTG
    TTAATGCAGG ACCCGGAGCG GTGGCCTCCT CTCTACGTCC TCTGGAAACT CAACAGAAAC
    CTCCCCACCC ACCTCCATTC CTGGAACCAC GCCCACCCCT TTACCCTGGA AGTTCTGGAG
    AAGATCTTGA ATTTCGTAGG CCTCAACGAG GTTCATAAAG CAATTTCCCT GATCATAGGA
    ATCTTACAGG AGCAGCCCTC CCCACCCCAG AGTATGCAGA GGGGAATATA CCGGGAACTG
    CTACTCCTAA CACTGGCTGC CCTCGGGAAG GATCACATGG ATATTGGTGC ATTTGATAAT
    AAATACAAGA CGGCCTTCAA GAAGCTAAGC AGCTCATTGG GGAAGGAAGA ACTGAGAAGA
    AGAAGGGCCC AGTTCCCCAC TCCCAAGGCG CTAGACTGTC GGAGAACCTT TGGGGCCACC
    CTGGATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746823.1
    CDS329..4840
    Translation

    Target ORF information:

    RefSeq Version XM_031890963.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890963.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAGAGG GACTGCCCAA ACTGGTGGAT TACTTTGTAG TGGCCGGCCT GACCCCGGCG 
    TCTCGGCCCT TGGAGGAAGA GAACCGCCAG CGCTCTATTC GCACCACAGA GCTGGTCACT
    GACGTGGCGG TCATTGTCAA GGCGCAGGGG GAGGAGGTTC CGCAGGGGTT TACCTGTATA
    GAGACCACCC CCGGGGGGCA CTCGGCCGAT CTGAATTCCG GCTTACTCAC CAACCAGCAG
    ATGTACCTGT GCTACCGCAG GGGCAGAGAC AAGCCCCCTA TCACCGAGTT AGGAGTGCAC
    TATGACGGCA AAGAACCTCT CCGCCCGGGC TTCCAGGTTA TCGACACCAC TCCGTACAGT
    CACTCTGCCA ACCTGAGCTC GGGGGGCCCC GGGAACCAGC GAGCCTTCCT GATGTTCAAA
    CGGGCCCCCG AATCCATGGG GCTGAATAGT CTGGGGGTGA TGGATATCTG TATCATTAAT
    CCCAGCAAGG GGGAAAGCAC CCCCAACACC TTTTGCCGAG TGGATAGGAA CCTCAATACA
    AGTATGTTCG GGCCGGCTCT GTTCCTCTGT TACAAGAAGG GCACGGCTAA GACCCACAGC
    CTTGTGTATG AGGCAGGGGT GCTCAGCCGC TTCCCTAGTG CCGACAGTGA AACGTTTCCC
    TTGCCCGAGA TGGTGGCGAC CTTCTGTCTG CCAATGGGTG CCACCATTGA GAGCTGGCCA
    ATCAACACTA AGTATCACAT GCCCGTCTTC TCCACCTTTG TGCTGACTGG GGCTTCTGGG
    GAGAAGGTAT ATGGCGCAGC CATCCAGTTC CATGAGCAAT ACCCCCGGGG GTGCCTGAGC
    GAGAAGCAGA ACCAATCCCT GGGGCTTCTG AGTGTGGTGG ACAAGCGCCC CGTGACCAAC
    AAATCTGTCC AGACCAAGAA GAGCATCTGT GTCCTCTCTC ATTGGCCCTT CTTCGACGTC
    TTCCAGAAGT TCCTCACTTT CATTTATCGA TACTCCATCT CCGGCCCCCA CGTCCTTCCC
    ATTGAGAAAC ATATCTCCAA CTTCATGTTC AATGTCCCCT TCCCGTCCCC ACAGCGGCCG
    CGCATTTTGG TTCAGATGTC CCCCTATGAT AACGTCCTGC TGTGCCAGCC AGTATCCTCT
    CCTTTGCCCC TCAGCGGAGC CAGTTACGTG ACTCTCCTGC AGAACCTGGG CCCCGAGACC
    GCCGTGACCC TACTCCTCGC CGTGCTGACC GAACACAAAC TGCTCATCCA CTCCCAGCGG
    CCCGATGTGC TGACCAGCGT GGGCGAGGCT TTGGTGTCGA TCATCTTCCC GCTGCGGTGG
    CAGTGCCCAT ACATCCCGCT GTGCCCCCTC ACCCTCTCCG ATGTGCTTTG TGCCCCTGTT
    CCGTTCATCG TGGGCATCCA CTCCAGCTAC TTCGACCTGC ACGACCCGCC CCAGGATGTT
    CTGTGTGTGG ATCTGGACAC CAACACCCTG TTCCAGAGTG AAGAGAAAAA GGTCCCATCG
    GTGCGCTCGC TGCCCCGCAA ACCCTGTAAG GTGCTACTGG GCACCCTGAG CGACCTGCAC
    AAACAGCTGG ACGAGATGTA CAACAAACCC ATGGAGGAGG CCACACTGGA GTTCCTGCTG
    AAAGACTACG ATCTGATCTA CGGACGCCGG ACGCAGTTGG AGCTGGACGT ACGCGCCGCC
    TTCCTGAGGT TCATGGCATG CTTATTAAAG GGGTACCGCT CCTTCCTGCT GCCCATCACC
    CAGGCCCCCT CTGAACGGAC GCGCGACTCC AGCAACCTGT TTAACGTGGA GGGTTTCCGG
    AAGTCGCGGG ACCGGGCGCA CCAGAAGTTC TACTCGCAGA TGCTGAAGAC ACAGATGTTC
    ACACAGTTTA TAGAGGACTG CTCCTTTGTT AGCGACCGGC ACGCCTGTCT CGAGTTCCTC
    GACAATTGCA TAGAAAAGGT GCAGATAGAT GGGGAGAAGA TGGAGGACTT ACACCTGGTG
    GAGTTGGACG AGTCCCACCG CAGCGAGCAC ACTGTGTTCA TCATGCCAGC CGAGGAACCT
    CATGAACCTG GCAGTGGGGA GCTGCCCGCC CAGTACATGT ATGAGAGCTT CCCAGTCCTC
    AAATGGGAGC TGTTCGACCT ACCCCAAGAT CTACTGATGG CTCCACCCAA CCAATCCAAG
    AGCAGCGCCC CGAGCAGCCC CGCCCCACGC CGGACCAAGC AGGAAATAAA AATGGCTCAG
    CGCGTGGCCC AGAAGTACTC AGCCATGCCC GACATGTGGG CCAAGTGCCT GCTGGGCCAC
    TGCTACGGCC TGTGGTTTAT TTACCTGCCC ACGTTCGTGC GTGCCACCCC CAGCAAAGTG
    CGAGCTCTTC AGACCGCCTA CGATGCCCTG AAGCAGATGG AGAATAAGAA GGTGGTCCTG
    CCTGATGAGG TGTGTTACCG GGTCCTGATG CAGCTGTGCG GCCAGTACGG GGAGCCGGTC
    CTAGCGGTGC GGGTTATGCT GGAAATGAAA AAAGCTGGCA TTGTGCCCAA CATCATCACC
    TACGGATACT ACAATAAGGC CATATTGGAA AGCAAATGGC CCTCGAGCAG TCAGAGCGGG
    CGCCTACGCT GGGCCAAGCT GCGCAATGTG GTTCTGGGAG CCGCACAGTT CCGCCAGCCC
    CTCAAACGGA AAAGGAGACA GGCCGGACTC AGCCTTTCCC AGACTGAAAG CCATTTGCCA
    AGGAACTGCG CCTCTGAGCT GCCCCCGCGG CTGAGCCTCC TGCGCCAAGC TACATGGGCG
    GGGAGCTCAC ACAGGGAATT CCATGAGAGC TGCGCCGGGA AACTGGTAAA GAGCTGGAGC
    GTCAGTGGCG CCGGGAGTGA TGGCGACATG GTGGAGAGCT CGTTGGCGCA CATGATGGAA
    GATGCGGAGG GTATGAAGAT GGGCAGAAGA GGAGAGGGAA GCCCGTCGCT AAGGAGGCCG
    TGTGGGACCC CCCTGGCCCT GGGCCCCTCG GAGAACGGGA GCCTATCCAA CGTCAGTCTG
    CAGACCGATG CCACCGGCAG CGACGTGGCT GTAACTTCAG AAGACTCGGA AGCCCTGATG
    ACGGAGAGTT CCACGGAGGC CTTGCCCTGC CGGCCGGCCA GAGACCTGGG AGACTCCTCC
    TCCACCGGTA CCAACGGCAA ACGGCACAGC TTGGCCGGCA AGATACAGCA GCTTCTCTCC
    CCGGCCCGGA AGCCTGGAAG GGTCACGTTA CTGCGCAAAA AGTCCTCCAT GTCTGAAAAA
    CCGTCCCCTC TCAGCTTCCC CGGGGATCAG AGCTGGAGGA GCTACAAGGA AAGCATTGGG
    CGCCCAGAGT CCACGGCCTC CGAGGTGAGT AAGAGCTCCG TCTCTCTGGG AAGCGAAGTG
    GACTTGTCCG ACACCTCCGT CTGCAGCTTC ACCCTACACA AATCCACCGA CCGACTGACC
    GACGGGGGGC AGGAAGCGCC GGCGGCAGAG GTGATGCTGT CCGGCTGCTC CCTGTGCCCG
    GTGTGCCACT CCCTGGTATA CGACGAGGAC ATCATGGCCG GCTGGACCTC CGATGACTCC
    AACCTGAAAA CCGTCTGCAC CTTCTGCGGC AAATACTTTG TGCCCCTCCT GAACGTCAGG
    GTCACCGACC TGCAGAGCCG GCACAGTGCT TCCAAGGACT CCTCTGCCTC CCCCCAGCCT
    TCCACAAATG GAGACTCCCC AGTCCCTGGG GGGGCGGGGC CAGTGCTGAG CGACCGCTTC
    CAGTGTTTGG TGCTCGATGA CCAAGGGACT GTGCGCCCCC CGTGCAATGG ATACACCGAC
    ACCCAATTAC AGCTGCCTGG GCCCAGTGCG AGCTCCGAGG TGGTTACGGT GGCCTACCTT
    TGCCCGCTGG TTCTGCGCAA GGAGCTGGAG AGCCTACTGG AGAATGAAGG TGGAGATGTC
    CTTTCCCAGC CAGAGCTGGT CAACAGCCAC CCAATCATCT ACTGGAACCT TGTGTGGTAC
    TTCAAGCGTA TGGCACTGCC CAGTAACCTC CTTCAGCTGG TGTTGGCCTC CAAACACGTC
    CGGCAGCAGT CACAGGTGCT GGAGGTGCCG CAGGTGAGGG TGCGACTTCT GTGGGACGTG
    TTAATGCAGG ACCCGGAGCG GTGGCCTCCT CTCTACGTCC TCTGGAAACT CAACAGAAAC
    CTCCCCACCC ACCTCCATTC CTGGAACCAC GCCCACCCCT TTACCCTGGA AGTTCTGGAG
    AAGATCTTGA ATTTCGTAGG CCTCAACGAG GTTCATAAAG CAATTTCCCT GATCATAGGA
    ATCTTACAGG AGCAGCCCTC CCCACCCCAG AGTATGCAGA GGGGAATATA CCGGGAACTG
    CTACTCCTAA CACTGGCTGC CCTCGGGAAG GATCACATGG ATATTGGTGC ATTTGATAAT
    AAATACAAGA CGGCCTTCAA GAAGCTAAGC AGCTCATTGG GGAAGGAAGA ACTGAGAAGA
    AGAAGGGCCC AGTTCCCCAC TCCCAAGGCG CTAGACTGTC GGAGAACCTT TGGGGCCACC
    CTGGATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64206
    Clone ID Related Accession (Same CDS sequence) XM_031890965.1
    Accession Version XM_031890965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DENN domain-containing protein 4B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAGAGG GACTGCCCAA ACTGGTGGAT TACTTTGTAG TGGCCGGCCT GACCCCGGCG 
    TCTCGGCCCT TGGAGGAAGA GAACCGCCAG CGCTCTATTC GCACCACAGA GCTGGTCACT
    GACGTGGCGG TCATTGTCAA GGCGCAGGGG GAGGAGGTTC CGCAGGGGTT TACCTGTATA
    GAGACCACCC CCGGGGGGCA CTCGGCCGAT CTGAATTCCG GCTTACTCAC CAACCAGCAG
    ATGTACCTGT GCTACCGCAG GGGCAGAGAC AAGCCCCCTA TCACCGAGTT AGGAGTGCAC
    TATGACGGCA AAGAACCTCT CCGCCCGGGC TTCCAGGTTA TCGACACCAC TCCGTACAGT
    CACTCTGCCA ACCTGAGCTC GGGGGGCCCC GGGAACCAGC GAGCCTTCCT GATGTTCAAA
    CGGGCCCCCG AATCCATGGG GCTGAATAGT CTGGGGGTGA TGGATATCTG TATCATTAAT
    CCCAGCAAGG GGGAAAGCAC CCCCAACACC TTTTGCCGAG TGGATAGGAA CCTCAATACA
    AGTATGTTCG GGCCGGCTCT GTTCCTCTGT TACAAGAAGG GCACGGCTAA GACCCACAGC
    CTTGTGTATG AGGCAGGGGT GCTCAGCCGC TTCCCTAGTG CCGACAGTGA AACGTTTCCC
    TTGCCCGAGA TGGTGGCGAC CTTCTGTCTG CCAATGGGTG CCACCATTGA GAGCTGGCCA
    ATCAACACTA AGTATCACAT GCCCGTCTTC TCCACCTTTG TGCTGACTGG GGCTTCTGGG
    GAGAAGGTAT ATGGCGCAGC CATCCAGTTC CATGAGCAAT ACCCCCGGGG GTGCCTGAGC
    GAGAAGCAGA ACCAATCCCT GGGGCTTCTG AGTGTGGTGG ACAAGCGCCC CGTGACCAAC
    AAATCTGTCC AGACCAAGAA GAGCATCTGT GTCCTCTCTC ATTGGCCCTT CTTCGACGTC
    TTCCAGAAGT TCCTCACTTT CATTTATCGA TACTCCATCT CCGGCCCCCA CGTCCTTCCC
    ATTGAGAAAC ATATCTCCAA CTTCATGTTC AATGTCCCCT TCCCGTCCCC ACAGCGGCCG
    CGCATTTTGG TTCAGATGTC CCCCTATGAT AACGTCCTGC TGTGCCAGCC AGTATCCTCT
    CCTTTGCCCC TCAGCGGAGC CAGTTACGTG ACTCTCCTGC AGAACCTGGG CCCCGAGACC
    GCCGTGACCC TACTCCTCGC CGTGCTGACC GAACACAAAC TGCTCATCCA CTCCCAGCGG
    CCCGATGTGC TGACCAGCGT GGGCGAGGCT TTGGTGTCGA TCATCTTCCC GCTGCGGTGG
    CAGTGCCCAT ACATCCCGCT GTGCCCCCTC ACCCTCTCCG ATGTGCTTTG TGCCCCTGTT
    CCGTTCATCG TGGGCATCCA CTCCAGCTAC TTCGACCTGC ACGACCCGCC CCAGGATGTT
    CTGTGTGTGG ATCTGGACAC CAACACCCTG TTCCAGAGTG AAGAGAAAAA GGTCCCATCG
    GTGCGCTCGC TGCCCCGCAA ACCCTGTAAG GTGCTACTGG GCACCCTGAG CGACCTGCAC
    AAACAGCTGG ACGAGATGTA CAACAAACCC ATGGAGGAGG CCACACTGGA GTTCCTGCTG
    AAAGACTACG ATCTGATCTA CGGACGCCGG ACGCAGTTGG AGCTGGACGT ACGCGCCGCC
    TTCCTGAGGT TCATGGCATG CTTATTAAAG GGGTACCGCT CCTTCCTGCT GCCCATCACC
    CAGGCCCCCT CTGAACGGAC GCGCGACTCC AGCAACCTGT TTAACGTGGA GGGTTTCCGG
    AAGTCGCGGG ACCGGGCGCA CCAGAAGTTC TACTCGCAGA TGCTGAAGAC ACAGATGTTC
    ACACAGTTTA TAGAGGACTG CTCCTTTGTT AGCGACCGGC ACGCCTGTCT CGAGTTCCTC
    GACAATTGCA TAGAAAAGGT GCAGATAGAT GGGGAGAAGA TGGAGGACTT ACACCTGGTG
    GAGTTGGACG AGTCCCACCG CAGCGAGCAC ACTGTGTTCA TCATGCCAGC CGAGGAACCT
    CATGAACCTG GCAGTGGGGA GCTGCCCGCC CAGTACATGT ATGAGAGCTT CCCAGTCCTC
    AAATGGGAGC TGTTCGACCT ACCCCAAGAT CTACTGATGG CTCCACCCAA CCAATCCAAG
    AGCAGCGCCC CGAGCAGCCC CGCCCCACGC CGGACCAAGC AGGAAATAAA AATGGCTCAG
    CGCGTGGCCC AGAAGTACTC AGCCATGCCC GACATGTGGG CCAAGTGCCT GCTGGGCCAC
    TGCTACGGCC TGTGGTTTAT TTACCTGCCC ACGTTCGTGC GTGCCACCCC CAGCAAAGTG
    CGAGCTCTTC AGACCGCCTA CGATGCCCTG AAGCAGATGG AGAATAAGAA GGTGGTCCTG
    CCTGATGAGG TGTGTTACCG GGTCCTGATG CAGCTGTGCG GCCAGTACGG GGAGCCGGTC
    CTAGCGGTGC GGGTTATGCT GGAAATGAAA AAAGCTGGCA TTGTGCCCAA CATCATCACC
    TACGGATACT ACAATAAGGC CATATTGGAA AGCAAATGGC CCTCGAGCAG TCAGAGCGGG
    CGCCTACGCT GGGCCAAGCT GCGCAATGTG GTTCTGGGAG CCGCACAGTT CCGCCAGCCC
    CTCAAACGGA AAAGGAGACA GGCCGGACTC AGCCTTTCCC AGACTGAAAG CCATTTGCCA
    AGGAACTGCG CCTCTGAGCT GCCCCCGCGG CTGAGCCTCC TGCGCCAAGC TACATGGGCG
    GGGAGCTCAC ACAGGGAATT CCATGAGAGC TGCGCCGGGA AACTGGTAAA GAGCTGGAGC
    GTCAGTGGCG CCGGGAGTGA TGGCGACATG GTGGAGAGCT CGTTGGCGCA CATGATGGAA
    GATGCGGAGG GTATGAAGAT GGGCAGAAGA GGAGAGGGAA GCCCGTCGCT AAGGAGGCCG
    TGTGGGACCC CCCTGGCCCT GGGCCCCTCG GAGAACGGGA GCCTATCCAA CGTCAGTCTG
    CAGACCGATG CCACCGGCAG CGACGTGGCT GTAACTTCAG AAGACTCGGA AGCCCTGATG
    ACGGAGAGTT CCACGGAGGC CTTGCCCTGC CGGCCGGCCA GAGACCTGGG AGACTCCTCC
    TCCACCGGTA CCAACGGCAA ACGGCACAGC TTGGCCGGCA AGATACAGCA GCTTCTCTCC
    CCGGCCCGGA AGCCTGGAAG GGTCACGTTA CTGCGCAAAA AGTCCTCCAT GTCTGAAAAA
    CCGTCCCCTC TCAGCTTCCC CGGGGATCAG AGCTGGAGGA GCTACAAGGA AAGCATTGGG
    CGCCCAGAGT CCACGGCCTC CGAGAGCTCC GTCTCTCTGG GAAGCGAAGT GGACTTGTCC
    GACACCTCCG TCTGCAGCTT CACCCTACAC AAATCCACCG ACCGACTGAC CGACGGGGGG
    CAGGAAGCGC CGGCGGCAGA GGTGATGCTG TCCGGCTGCT CCCTGTGCCC GGTGTGCCAC
    TCCCTGGTAT ACGACGAGGA CATCATGGCC GGCTGGACCT CCGATGACTC CAACCTGAAA
    ACCGTCTGCA CCTTCTGCGG CAAATACTTT GTGCCCCTCC TGAACGTCAG GGTCACCGAC
    CTGCAGAGCC GGCACAGTGC TTCCAAGGAC TCCTCTGCCT CCCCCCAGCC TTCCACAAAT
    GGAGACTCCC CAGTCCCTGG GGGGGCGGGG CCAGTGCTGA GCGACCGCTT CCAGTGTTTG
    GTGCTCGATG ACCAAGGGAC TGTGCGCCCC CCGTGCAATG GATACACCGA CACCCAATTA
    CAGCTGCCTG GGCCCAGTGC GAGCTCCGAG GTGGTTACGG TGGCCTACCT TTGCCCGCTG
    GTTCTGCGCA AGGAGCTGGA GAGCCTACTG GAGAATGAAG GTGGAGATGT CCTTTCCCAG
    CCAGAGCTGG TCAACAGCCA CCCAATCATC TACTGGAACC TTGTGTGGTA CTTCAAGCGT
    ATGGCACTGC CCAGTAACCT CCTTCAGCTG GTGTTGGCCT CCAAACACGT CCGGCAGCAG
    TCACAGGTGC TGGAGGTGCC GCAGGTGAGG GTGCGACTTC TGTGGGACGT GTTAATGCAG
    GACCCGGAGC GGTGGCCTCC TCTCTACGTC CTCTGGAAAC TCAACAGAAA CCTCCCCACC
    CACCTCCATT CCTGGAACCA CGCCCACCCC TTTACCCTGG AAGTTCTGGA GAAGATCTTG
    AATTTCGTAG GCCTCAACGA GGTTCATAAA GCAATTTCCC TGATCATAGG AATCTTACAG
    GAGCAGCCCT CCCCACCCCA GAGTATGCAG AGGGGAATAT ACCGGGAACT GCTACTCCTA
    ACACTGGCTG CCCTCGGGAA GGATCACATG GATATTGGTG CATTTGATAA TAAATACAAG
    ACGGCCTTCA AGAAGCTAAG CAGCTCATTG GGGAAGGAAG AACTGAGAAG AAGAAGGGCC
    CAGTTCCCCA CTCCCAAGGC GCTAGACTGT CGGAGAACCT TTGGGGCCAC CCTGGATTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746825.1
    CDS328..4830
    Translation

    Target ORF information:

    RefSeq Version XM_031890965.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DENN/MADD domain containing 4B (dennd4b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAGAGG GACTGCCCAA ACTGGTGGAT TACTTTGTAG TGGCCGGCCT GACCCCGGCG 
    TCTCGGCCCT TGGAGGAAGA GAACCGCCAG CGCTCTATTC GCACCACAGA GCTGGTCACT
    GACGTGGCGG TCATTGTCAA GGCGCAGGGG GAGGAGGTTC CGCAGGGGTT TACCTGTATA
    GAGACCACCC CCGGGGGGCA CTCGGCCGAT CTGAATTCCG GCTTACTCAC CAACCAGCAG
    ATGTACCTGT GCTACCGCAG GGGCAGAGAC AAGCCCCCTA TCACCGAGTT AGGAGTGCAC
    TATGACGGCA AAGAACCTCT CCGCCCGGGC TTCCAGGTTA TCGACACCAC TCCGTACAGT
    CACTCTGCCA ACCTGAGCTC GGGGGGCCCC GGGAACCAGC GAGCCTTCCT GATGTTCAAA
    CGGGCCCCCG AATCCATGGG GCTGAATAGT CTGGGGGTGA TGGATATCTG TATCATTAAT
    CCCAGCAAGG GGGAAAGCAC CCCCAACACC TTTTGCCGAG TGGATAGGAA CCTCAATACA
    AGTATGTTCG GGCCGGCTCT GTTCCTCTGT TACAAGAAGG GCACGGCTAA GACCCACAGC
    CTTGTGTATG AGGCAGGGGT GCTCAGCCGC TTCCCTAGTG CCGACAGTGA AACGTTTCCC
    TTGCCCGAGA TGGTGGCGAC CTTCTGTCTG CCAATGGGTG CCACCATTGA GAGCTGGCCA
    ATCAACACTA AGTATCACAT GCCCGTCTTC TCCACCTTTG TGCTGACTGG GGCTTCTGGG
    GAGAAGGTAT ATGGCGCAGC CATCCAGTTC CATGAGCAAT ACCCCCGGGG GTGCCTGAGC
    GAGAAGCAGA ACCAATCCCT GGGGCTTCTG AGTGTGGTGG ACAAGCGCCC CGTGACCAAC
    AAATCTGTCC AGACCAAGAA GAGCATCTGT GTCCTCTCTC ATTGGCCCTT CTTCGACGTC
    TTCCAGAAGT TCCTCACTTT CATTTATCGA TACTCCATCT CCGGCCCCCA CGTCCTTCCC
    ATTGAGAAAC ATATCTCCAA CTTCATGTTC AATGTCCCCT TCCCGTCCCC ACAGCGGCCG
    CGCATTTTGG TTCAGATGTC CCCCTATGAT AACGTCCTGC TGTGCCAGCC AGTATCCTCT
    CCTTTGCCCC TCAGCGGAGC CAGTTACGTG ACTCTCCTGC AGAACCTGGG CCCCGAGACC
    GCCGTGACCC TACTCCTCGC CGTGCTGACC GAACACAAAC TGCTCATCCA CTCCCAGCGG
    CCCGATGTGC TGACCAGCGT GGGCGAGGCT TTGGTGTCGA TCATCTTCCC GCTGCGGTGG
    CAGTGCCCAT ACATCCCGCT GTGCCCCCTC ACCCTCTCCG ATGTGCTTTG TGCCCCTGTT
    CCGTTCATCG TGGGCATCCA CTCCAGCTAC TTCGACCTGC ACGACCCGCC CCAGGATGTT
    CTGTGTGTGG ATCTGGACAC CAACACCCTG TTCCAGAGTG AAGAGAAAAA GGTCCCATCG
    GTGCGCTCGC TGCCCCGCAA ACCCTGTAAG GTGCTACTGG GCACCCTGAG CGACCTGCAC
    AAACAGCTGG ACGAGATGTA CAACAAACCC ATGGAGGAGG CCACACTGGA GTTCCTGCTG
    AAAGACTACG ATCTGATCTA CGGACGCCGG ACGCAGTTGG AGCTGGACGT ACGCGCCGCC
    TTCCTGAGGT TCATGGCATG CTTATTAAAG GGGTACCGCT CCTTCCTGCT GCCCATCACC
    CAGGCCCCCT CTGAACGGAC GCGCGACTCC AGCAACCTGT TTAACGTGGA GGGTTTCCGG
    AAGTCGCGGG ACCGGGCGCA CCAGAAGTTC TACTCGCAGA TGCTGAAGAC ACAGATGTTC
    ACACAGTTTA TAGAGGACTG CTCCTTTGTT AGCGACCGGC ACGCCTGTCT CGAGTTCCTC
    GACAATTGCA TAGAAAAGGT GCAGATAGAT GGGGAGAAGA TGGAGGACTT ACACCTGGTG
    GAGTTGGACG AGTCCCACCG CAGCGAGCAC ACTGTGTTCA TCATGCCAGC CGAGGAACCT
    CATGAACCTG GCAGTGGGGA GCTGCCCGCC CAGTACATGT ATGAGAGCTT CCCAGTCCTC
    AAATGGGAGC TGTTCGACCT ACCCCAAGAT CTACTGATGG CTCCACCCAA CCAATCCAAG
    AGCAGCGCCC CGAGCAGCCC CGCCCCACGC CGGACCAAGC AGGAAATAAA AATGGCTCAG
    CGCGTGGCCC AGAAGTACTC AGCCATGCCC GACATGTGGG CCAAGTGCCT GCTGGGCCAC
    TGCTACGGCC TGTGGTTTAT TTACCTGCCC ACGTTCGTGC GTGCCACCCC CAGCAAAGTG
    CGAGCTCTTC AGACCGCCTA CGATGCCCTG AAGCAGATGG AGAATAAGAA GGTGGTCCTG
    CCTGATGAGG TGTGTTACCG GGTCCTGATG CAGCTGTGCG GCCAGTACGG GGAGCCGGTC
    CTAGCGGTGC GGGTTATGCT GGAAATGAAA AAAGCTGGCA TTGTGCCCAA CATCATCACC
    TACGGATACT ACAATAAGGC CATATTGGAA AGCAAATGGC CCTCGAGCAG TCAGAGCGGG
    CGCCTACGCT GGGCCAAGCT GCGCAATGTG GTTCTGGGAG CCGCACAGTT CCGCCAGCCC
    CTCAAACGGA AAAGGAGACA GGCCGGACTC AGCCTTTCCC AGACTGAAAG CCATTTGCCA
    AGGAACTGCG CCTCTGAGCT GCCCCCGCGG CTGAGCCTCC TGCGCCAAGC TACATGGGCG
    GGGAGCTCAC ACAGGGAATT CCATGAGAGC TGCGCCGGGA AACTGGTAAA GAGCTGGAGC
    GTCAGTGGCG CCGGGAGTGA TGGCGACATG GTGGAGAGCT CGTTGGCGCA CATGATGGAA
    GATGCGGAGG GTATGAAGAT GGGCAGAAGA GGAGAGGGAA GCCCGTCGCT AAGGAGGCCG
    TGTGGGACCC CCCTGGCCCT GGGCCCCTCG GAGAACGGGA GCCTATCCAA CGTCAGTCTG
    CAGACCGATG CCACCGGCAG CGACGTGGCT GTAACTTCAG AAGACTCGGA AGCCCTGATG
    ACGGAGAGTT CCACGGAGGC CTTGCCCTGC CGGCCGGCCA GAGACCTGGG AGACTCCTCC
    TCCACCGGTA CCAACGGCAA ACGGCACAGC TTGGCCGGCA AGATACAGCA GCTTCTCTCC
    CCGGCCCGGA AGCCTGGAAG GGTCACGTTA CTGCGCAAAA AGTCCTCCAT GTCTGAAAAA
    CCGTCCCCTC TCAGCTTCCC CGGGGATCAG AGCTGGAGGA GCTACAAGGA AAGCATTGGG
    CGCCCAGAGT CCACGGCCTC CGAGAGCTCC GTCTCTCTGG GAAGCGAAGT GGACTTGTCC
    GACACCTCCG TCTGCAGCTT CACCCTACAC AAATCCACCG ACCGACTGAC CGACGGGGGG
    CAGGAAGCGC CGGCGGCAGA GGTGATGCTG TCCGGCTGCT CCCTGTGCCC GGTGTGCCAC
    TCCCTGGTAT ACGACGAGGA CATCATGGCC GGCTGGACCT CCGATGACTC CAACCTGAAA
    ACCGTCTGCA CCTTCTGCGG CAAATACTTT GTGCCCCTCC TGAACGTCAG GGTCACCGAC
    CTGCAGAGCC GGCACAGTGC TTCCAAGGAC TCCTCTGCCT CCCCCCAGCC TTCCACAAAT
    GGAGACTCCC CAGTCCCTGG GGGGGCGGGG CCAGTGCTGA GCGACCGCTT CCAGTGTTTG
    GTGCTCGATG ACCAAGGGAC TGTGCGCCCC CCGTGCAATG GATACACCGA CACCCAATTA
    CAGCTGCCTG GGCCCAGTGC GAGCTCCGAG GTGGTTACGG TGGCCTACCT TTGCCCGCTG
    GTTCTGCGCA AGGAGCTGGA GAGCCTACTG GAGAATGAAG GTGGAGATGT CCTTTCCCAG
    CCAGAGCTGG TCAACAGCCA CCCAATCATC TACTGGAACC TTGTGTGGTA CTTCAAGCGT
    ATGGCACTGC CCAGTAACCT CCTTCAGCTG GTGTTGGCCT CCAAACACGT CCGGCAGCAG
    TCACAGGTGC TGGAGGTGCC GCAGGTGAGG GTGCGACTTC TGTGGGACGT GTTAATGCAG
    GACCCGGAGC GGTGGCCTCC TCTCTACGTC CTCTGGAAAC TCAACAGAAA CCTCCCCACC
    CACCTCCATT CCTGGAACCA CGCCCACCCC TTTACCCTGG AAGTTCTGGA GAAGATCTTG
    AATTTCGTAG GCCTCAACGA GGTTCATAAA GCAATTTCCC TGATCATAGG AATCTTACAG
    GAGCAGCCCT CCCCACCCCA GAGTATGCAG AGGGGAATAT ACCGGGAACT GCTACTCCTA
    ACACTGGCTG CCCTCGGGAA GGATCACATG GATATTGGTG CATTTGATAA TAAATACAAG
    ACGGCCTTCA AGAAGCTAAG CAGCTCATTG GGGAAGGAAG AACTGAGAAG AAGAAGGGCC
    CAGTTCCCCA CTCCCAAGGC GCTAGACTGT CGGAGAACCT TTGGGGCCAC CCTGGATTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
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