magi2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	magi2
Entrez Gene ID	100101686
Full Name	membrane associated guanylate kinase, WW and PDZ domain containing 2
Synonyms	acvrip1,aip1,arip1,magi-2,s-scam,sscam
General protein information	Preferred Names membrane associated guanylate kinase, WW and PDZ domain containing 2 Names membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031897512.1	XP_031753372.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X2
NM_001100212.1	NP_001093682.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
XM_031897516.1	XP_031753376.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X6
XM_031897513.1	XP_031753373.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X3
NM_001374763.1	NP_001361692.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
XM_031897515.1	XP_031753375.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X5
XM_031897511.1	XP_031753371.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X1
XM_031897514.1	XP_031753374.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4

Pathways from BioSystems

Homologies

	LOC709228	XP_001108762.1
Xenopus tropicalis (tropical clawed frog)	magi2	NP_001093682.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following magi2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the magi2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22774	NM_001100212.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OXa47269	NM_001374763.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa53137	XM_031897511.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53138	XM_031897512.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53139	XM_031897513.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53140	XM_031897514.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53141	XM_031897515.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53142	XM_031897516.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

magi2 ( NM_001100212.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001093682.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), mRNA.

Price & Availability↑

CloneID	OXa22774
Clone ID Related Accession (Same CDS sequence)	NM_001100212.1
Accession Version	NM_001100212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC136221.1. ##Evidence-Data-START## Transcript exon combination :: BC136221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1696413, SAMN00861997 [ECO:0000350] ##Evidence-Data-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGGTAGA 
TATTCTGGCT TTTACAGAAC TATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093682.1
CDS	261..827
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEGRYSGFYRTI

Target ORF information:

RefSeq Version	NM_001100212.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100212.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGGTAGA 
TATTCTGGCT TTTACAGAAC TATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( NM_001374763.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001361692.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), mRNA.

Price & Availability↑

CloneID	OXa47269
Clone ID Related Accession (Same CDS sequence)	NM_001374763.1
Accession Version	NM_001374763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03011793.1, AAMC03011790.1, AAMC03011788.1, AAMC03011787.1 and AAMC03011786.1. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGCAGATAC ATGTCCAGAA AATGGCAGGA GTCCTGGCTC TGCCTCAACA 
CAACACAGTT CTCCCCGAAG TGATTCTGCT TATCCAAACA GCAACCATTC TGCACCATGC 
AATCCTTCTC CACCAGAGGG TTTCACGTCA CAAAGCATGC AGTCCAGTGA TGTGGTTATA 
CATCGAAAGG AGAATGAAGG ATTTGGTTTT GTCATAATAA GTTCTTTGAA CAGACCAGAA 
TCAGGCGCAA CAATTGCTGT GCCACATAAA ATCGGACGAA TTATTGAAGG AAGCCCTGCA 
GATCGCTGTG GAAAGCTAAA AGTCGGAGAC CGGATCTTAG CTGTGAATAG CCAATCTATT 
ATCAACATGC CTCATGCAGA TATTGTAAAG CTGATTAAGG ATGCAGGGCT GAGTGTAACA 
CTTTGTATCG TTCCTCAGGA GGAATTAAAC AGTCCAGCTT CAGCACCAAG CTCAGAAAAA 
CAGAGTCCTA TGGCACAGCA GCACAGCCCA ATGGCACAGC AGAGCCCCGT AGCTCAGCAA 
AGTCCTGTGG CACACTCACT GCCCCCTCAA CCACTACAGT TGCAAGGCCA TGAGAGCAGC 
TATAGGTCAG AAGTAAAAGC AAGGCAAGAT GTAAAACCAG ACATCAGGCA ACCACCATTT 
ACAGATTATC GCCAGCCATC AACGGACTAT AGGCAGCCTC CTGTAGATTA CAGACATCCT 
CCAGTAGTGG ATTACCAGCA GCCTCCTCCT ATAGACTACA GACAGCCACC TTTAATAGAG 
TACAGGCAAC ATTCTCCCGA TACTCGGCAA TTTCCTCTCC CTGATTACCG ACAATTTCAG 
GATTATGATT ATTTTACTGT TGAACTGGAA AAGGGTGCTA AGGGTTTTGG ATTTAGTATA 
CGAGGAGGAA GAGAATACAA GATGGATTTG TATGTGTTAA GGCTGGCAGA AGATGGCCCT 
GCAATAAGAA ATGGACGAAT GAGGGTTGGT GATCAAATCA TAGAAATCAA TGGGGAAAGC 
ACAAGAGACA TGACACATGC CAGAGCAATA GAACTCATTA AATCAGGAGG AAGAAGAGTA 
AAGCTTTTAC TGAAGCGTGG AACAGGTCAT GTTCCTGAGT ATGGAATGGT TCCTTCTTCC 
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC 
CACCCTAAAG ACACGACGAA CCAAACTCCT GGAGTTCTCC CTCTACCACC TCCCCAGGGC 
CGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361692.1
CDS	357..4205
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTGFRVDGVGPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIADTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYGMVPSSSLSMCMKSDKHGSPYFYLLGHPKDTTNQTPGVLPLPPPQGRK

Target ORF information:

RefSeq Version	NM_001374763.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374763.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGCAGATAC ATGTCCAGAA AATGGCAGGA GTCCTGGCTC TGCCTCAACA 
CAACACAGTT CTCCCCGAAG TGATTCTGCT TATCCAAACA GCAACCATTC TGCACCATGC 
AATCCTTCTC CACCAGAGGG TTTCACGTCA CAAAGCATGC AGTCCAGTGA TGTGGTTATA 
CATCGAAAGG AGAATGAAGG ATTTGGTTTT GTCATAATAA GTTCTTTGAA CAGACCAGAA 
TCAGGCGCAA CAATTGCTGT GCCACATAAA ATCGGACGAA TTATTGAAGG AAGCCCTGCA 
GATCGCTGTG GAAAGCTAAA AGTCGGAGAC CGGATCTTAG CTGTGAATAG CCAATCTATT 
ATCAACATGC CTCATGCAGA TATTGTAAAG CTGATTAAGG ATGCAGGGCT GAGTGTAACA 
CTTTGTATCG TTCCTCAGGA GGAATTAAAC AGTCCAGCTT CAGCACCAAG CTCAGAAAAA 
CAGAGTCCTA TGGCACAGCA GCACAGCCCA ATGGCACAGC AGAGCCCCGT AGCTCAGCAA 
AGTCCTGTGG CACACTCACT GCCCCCTCAA CCACTACAGT TGCAAGGCCA TGAGAGCAGC 
TATAGGTCAG AAGTAAAAGC AAGGCAAGAT GTAAAACCAG ACATCAGGCA ACCACCATTT 
ACAGATTATC GCCAGCCATC AACGGACTAT AGGCAGCCTC CTGTAGATTA CAGACATCCT 
CCAGTAGTGG ATTACCAGCA GCCTCCTCCT ATAGACTACA GACAGCCACC TTTAATAGAG 
TACAGGCAAC ATTCTCCCGA TACTCGGCAA TTTCCTCTCC CTGATTACCG ACAATTTCAG 
GATTATGATT ATTTTACTGT TGAACTGGAA AAGGGTGCTA AGGGTTTTGG ATTTAGTATA 
CGAGGAGGAA GAGAATACAA GATGGATTTG TATGTGTTAA GGCTGGCAGA AGATGGCCCT 
GCAATAAGAA ATGGACGAAT GAGGGTTGGT GATCAAATCA TAGAAATCAA TGGGGAAAGC 
ACAAGAGACA TGACACATGC CAGAGCAATA GAACTCATTA AATCAGGAGG AAGAAGAGTA 
AAGCTTTTAC TGAAGCGTGG AACAGGTCAT GTTCCTGAGT ATGGAATGGT TCCTTCTTCC 
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC 
CACCCTAAAG ACACGACGAA CCAAACTCCT GGAGTTCTCC CTCTACCACC TCCCCAGGGC 
CGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( XM_031897511.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753371.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53137
Clone ID Related Accession (Same CDS sequence)	XM_031897511.1
Accession Version	XM_031897511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGCAGATAC ATGTCCAGAA AATGGCAGGA GTCCTGGCTC TGCCTCAACA 
CAACACAGTT CTCCCCGAAG TGATTCTGCT TATCCAAACA GCAACCATTC TGCACCATGC 
AATCCTTCTC CACCAGAGGG TTTCACGTCA CAAAGCATGC AGTCCAGTGA TGTGGTTATA 
CATCGAAAGG AGAATGAAGG ATTTGGTTTT GTCATAATAA GTTCTTTGAA CAGACCAGAA 
TCAGGCGCAA CAATTGCTGT GCCACATAAA ATCGGACGAA TTATTGAAGG AAGCCCTGCA 
GATCGCTGTG GAAAGCTAAA AGTCGGAGAC CGGATCTTAG CTGTGAATAG CCAATCTATT 
ATCAACATGC CTCATGCAGA TATTGTAAAG CTGATTAAGG ATGCAGGGCT GAGTGTAACA 
CTTTGTATCG TTCCTCAGGA GGAATTAAAC AGTCCAGCTT CAGCACCAAG CTCAGAAAAA 
CAGAGTCCTA TGGCACAGCA GCACAGCCCA ATGGCACAGC AGAGCCCCGT AGCTCAGCAA 
AGTCCTGTGG CACACTCACT GCCCCCTCAA CCACTACAGT TGCAAGGCCA TGAGAGCAGC 
TATAGGTCAG AAGTAAAAGC AAGGCAAGAT GTAAAACCAG ACATCAGGCA ACCACCATTT 
ACAGATTATC GCCAGCCATC AACGGACTAT AGGCAGCCTC CTGTAGATTA CAGACATCCT 
CCAGTAGTGG ATTACCAGCA GCCTCCTCCT ATAGACTACA GACAGCCACC TTTAATAGAG 
TACAGGCAAC ATTCTCCCGA TACTCGGCAA TTTCCTCTCC CTGATTACCG ACAATTTCAG 
GATTATGATT ATTTTACTGT TGAACTGGAA AAGGGTGCTA AGGGTTTTGG ATTTAGTATA 
CGAGGAGGAA GAGAATACAA GATGGATTTG TATGTGTTAA GGCTGGCAGA AGATGGCCCT 
GCAATAAGAA ATGGACGAAT GAGGGTTGGT GATCAAATCA TAGAAATCAA TGGGGAAAGC 
ACAAGAGACA TGACACATGC CAGAGCAATA GAACTCATTA AATCAGGAGG AAGAAGAGTA 
AAGCTTTTAC TGAAGCGTGG AACAGGTCAT GTTCCTGAGT ATGACGAACC AAACTCCTGG 
AGTTCTCCCT CTACCACCTC CCCAGGGCCG GAAGTAGGTG TTTCCCTTGA TGACATAATT 
GCACCATTAT CTTCATCACA TTCAGCCTTG CCCTTTGACC CTACTCACCA GATACACCCA 
GAGCCAGCAT GGGATATCTA CAGGGAACAA GATATTGGAA AACCAAAGGA GAATGCTGCA 
AATGGTTATA AAAAGAAAAG ACTGGGAGAG TCGAGGGAGA GGTCAGCAAG CCCTAAGAAA 
GTAGACCGAT CAATGCGTGA AGAGAATTTC AAAAAGGAAT TTCAGGAAAG CAAACAGAAA 
AGAGCAGAAA ATAGCAAAAC CACCTCAGTG GGCAAAGTGA CTGTAAAAAG GGTGTTAATC 
GAAGAAGATT GTGGATATTC TACAACCGGT GATAGTGGAG GTCGGACATC CTGGTCCGCT 
GAAACAAAAA GCGAAGGAAC TTTATGTGCA AAAGACGTAC ACAAAGATGC AACCAGTGAG 
AAAAAGTCAG TGTCAGGTCT TGAGCAAAGC AAGTCAGATC CTAGTGTCAT CTCCAAGGTG 
TACAGTAGCA ATATAACCAG CTCTCGAGCG AGTGCCATGG ACCAAGATGA GAGAGATGCT 
GCAGCTAAAT GCAATGACCT TCAAAAAGAA GGGCCCCTCC ATTCCACCAA CACTCGCAGT 
GTCAGCACTG CAGCTCGGAA AGCAACAGTC TCACCAGGAC CGTGGAAAAT TCCTGGCTCT 
GATAAGCTAC CTAGTACCCT GAAATATGGC ACTTCAGCCT TGAGTAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753371.1
CDS	354..4844
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTGFRVDGVGPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIADTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYDEPNSWSSPSTTSPGPEVGVSLDDIIAPLSSSHSALPFDPTHQIHPEPAWDIYREQDIGKPKENAANGYKKKRLGESRERSASPKKVDRSMREENFKKEFQESKQKRAENSKTTSVGKVTVKRVLIEEDCGYSTTGDSGGRTSWSAETKSEGTLCAKDVHKDATSEKKSVSGLEQSKSDPSVISKVYSSNITSSRASAMDQDERDAAAKCNDLQKEGPLHSTNTRSVSTAARKATVSPGPWKIPGSDKLPSTLKYGTSALSR

Target ORF information:

RefSeq Version	XM_031897511.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897511.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGCAGATAC ATGTCCAGAA AATGGCAGGA GTCCTGGCTC TGCCTCAACA 
CAACACAGTT CTCCCCGAAG TGATTCTGCT TATCCAAACA GCAACCATTC TGCACCATGC 
AATCCTTCTC CACCAGAGGG TTTCACGTCA CAAAGCATGC AGTCCAGTGA TGTGGTTATA 
CATCGAAAGG AGAATGAAGG ATTTGGTTTT GTCATAATAA GTTCTTTGAA CAGACCAGAA 
TCAGGCGCAA CAATTGCTGT GCCACATAAA ATCGGACGAA TTATTGAAGG AAGCCCTGCA 
GATCGCTGTG GAAAGCTAAA AGTCGGAGAC CGGATCTTAG CTGTGAATAG CCAATCTATT 
ATCAACATGC CTCATGCAGA TATTGTAAAG CTGATTAAGG ATGCAGGGCT GAGTGTAACA 
CTTTGTATCG TTCCTCAGGA GGAATTAAAC AGTCCAGCTT CAGCACCAAG CTCAGAAAAA 
CAGAGTCCTA TGGCACAGCA GCACAGCCCA ATGGCACAGC AGAGCCCCGT AGCTCAGCAA 
AGTCCTGTGG CACACTCACT GCCCCCTCAA CCACTACAGT TGCAAGGCCA TGAGAGCAGC 
TATAGGTCAG AAGTAAAAGC AAGGCAAGAT GTAAAACCAG ACATCAGGCA ACCACCATTT 
ACAGATTATC GCCAGCCATC AACGGACTAT AGGCAGCCTC CTGTAGATTA CAGACATCCT 
CCAGTAGTGG ATTACCAGCA GCCTCCTCCT ATAGACTACA GACAGCCACC TTTAATAGAG 
TACAGGCAAC ATTCTCCCGA TACTCGGCAA TTTCCTCTCC CTGATTACCG ACAATTTCAG 
GATTATGATT ATTTTACTGT TGAACTGGAA AAGGGTGCTA AGGGTTTTGG ATTTAGTATA 
CGAGGAGGAA GAGAATACAA GATGGATTTG TATGTGTTAA GGCTGGCAGA AGATGGCCCT 
GCAATAAGAA ATGGACGAAT GAGGGTTGGT GATCAAATCA TAGAAATCAA TGGGGAAAGC 
ACAAGAGACA TGACACATGC CAGAGCAATA GAACTCATTA AATCAGGAGG AAGAAGAGTA 
AAGCTTTTAC TGAAGCGTGG AACAGGTCAT GTTCCTGAGT ATGACGAACC AAACTCCTGG 
AGTTCTCCCT CTACCACCTC CCCAGGGCCG GAAGTAGGTG TTTCCCTTGA TGACATAATT 
GCACCATTAT CTTCATCACA TTCAGCCTTG CCCTTTGACC CTACTCACCA GATACACCCA 
GAGCCAGCAT GGGATATCTA CAGGGAACAA GATATTGGAA AACCAAAGGA GAATGCTGCA 
AATGGTTATA AAAAGAAAAG ACTGGGAGAG TCGAGGGAGA GGTCAGCAAG CCCTAAGAAA 
GTAGACCGAT CAATGCGTGA AGAGAATTTC AAAAAGGAAT TTCAGGAAAG CAAACAGAAA 
AGAGCAGAAA ATAGCAAAAC CACCTCAGTG GGCAAAGTGA CTGTAAAAAG GGTGTTAATC 
GAAGAAGATT GTGGATATTC TACAACCGGT GATAGTGGAG GTCGGACATC CTGGTCCGCT 
GAAACAAAAA GCGAAGGAAC TTTATGTGCA AAAGACGTAC ACAAAGATGC AACCAGTGAG 
AAAAAGTCAG TGTCAGGTCT TGAGCAAAGC AAGTCAGATC CTAGTGTCAT CTCCAAGGTG 
TACAGTAGCA ATATAACCAG CTCTCGAGCG AGTGCCATGG ACCAAGATGA GAGAGATGCT 
GCAGCTAAAT GCAATGACCT TCAAAAAGAA GGGCCCCTCC ATTCCACCAA CACTCGCAGT 
GTCAGCACTG CAGCTCGGAA AGCAACAGTC TCACCAGGAC CGTGGAAAAT TCCTGGCTCT 
GATAAGCTAC CTAGTACCCT GAAATATGGC ACTTCAGCCT TGAGTAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( XM_031897512.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753372.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa53138
Clone ID Related Accession (Same CDS sequence)	XM_031897512.1
Accession Version	XM_031897512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGATACATG TCCAGAAAAT GGCAGGAGTC CTGGCTCTGC CTCAACACAA 
CACAGTTCTC CCCGAAGTGA TTCTGCTTAT CCAAACAGCA ACCATTCTGC ACCATGCAAT 
CCTTCTCCAC CAGAGGGTTT CACGTCACAA AGCATGCAGT CCAGTGATGT GGTTATACAT 
CGAAAGGAGA ATGAAGGATT TGGTTTTGTC ATAATAAGTT CTTTGAACAG ACCAGAATCA 
GGCGCAACAA TTGCTGTGCC ACATAAAATC GGACGAATTA TTGAAGGAAG CCCTGCAGAT 
CGCTGTGGAA AGCTAAAAGT CGGAGACCGG ATCTTAGCTG TGAATAGCCA ATCTATTATC 
AACATGCCTC ATGCAGATAT TGTAAAGCTG ATTAAGGATG CAGGGCTGAG TGTAACACTT 
TGTATCGTTC CTCAGGAGGA ATTAAACAGT CCAGCTTCAG CACCAAGCTC AGAAAAACAG 
AGTCCTATGG CACAGCAGCA CAGCCCAATG GCACAGCAGA GCCCCGTAGC TCAGCAAAGT 
CCTGTGGCAC ACTCACTGCC CCCTCAACCA CTACAGTTGC AAGGCCATGA GAGCAGCTAT 
AGGTCAGAAG TAAAAGCAAG GCAAGATGTA AAACCAGACA TCAGGCAACC ACCATTTACA 
GATTATCGCC AGCCATCAAC GGACTATAGG CAGCCTCCTG TAGATTACAG ACATCCTCCA 
GTAGTGGATT ACCAGCAGCC TCCTCCTATA GACTACAGAC AGCCACCTTT AATAGAGTAC 
AGGCAACATT CTCCCGATAC TCGGCAATTT CCTCTCCCTG ATTACCGACA ATTTCAGGAT 
TATGATTATT TTACTGTTGA ACTGGAAAAG GGTGCTAAGG GTTTTGGATT TAGTATACGA 
GGAGGAAGAG AATACAAGAT GGATTTGTAT GTGTTAAGGC TGGCAGAAGA TGGCCCTGCA 
ATAAGAAATG GACGAATGAG GGTTGGTGAT CAAATCATAG AAATCAATGG GGAAAGCACA 
AGAGACATGA CACATGCCAG AGCAATAGAA CTCATTAAAT CAGGAGGAAG AAGAGTAAAG 
CTTTTACTGA AGCGTGGAAC AGGTCATGTT CCTGAGTATG ACGAACCAAA CTCCTGGAGT 
TCTCCCTCTA CCACCTCCCC AGGGCCGGAA GTAGGTGTTT CCCTTGATGA CATAATTGCA 
CCATTATCTT CATCACATTC AGCCTTGCCC TTTGACCCTA CTCACCAGAT ACACCCAGAG 
CCAGCATGGG ATATCTACAG GGAACAAGAT ATTGGAAAAC CAAAGGAGAA TGCTGCAAAT 
GGTTATAAAA AGAAAAGACT GGGAGAGTCG AGGGAGAGGT CAGCAAGCCC TAAGAAAGTA 
GACCGATCAA TGCGTGAAGA GAATTTCAAA AAGGAATTTC AGGAAAGCAA ACAGAAAAGA 
GCAGAAAATA GCAAAACCAC CTCAGTGGGC AAAGTGACTG TAAAAAGGGT GTTAATCGAA 
GAAGATTGTG GATATTCTAC AACCGGTGAT AGTGGAGGTC GGACATCCTG GTCCGCTGAA 
ACAAAAAGCG AAGGAACTTT ATGTGCAAAA GACGTACACA AAGATGCAAC CAGTGAGAAA 
AAGTCAGTGT CAGGTCTTGA GCAAAGCAAG TCAGATCCTA GTGTCATCTC CAAGGTGTAC 
AGTAGCAATA TAACCAGCTC TCGAGCGAGT GCCATGGACC AAGATGAGAG AGATGCTGCA 
GCTAAATGCA ATGACCTTCA AAAAGAAGGG CCCCTCCATT CCACCAACAC TCGCAGTGTC 
AGCACTGCAG CTCGGAAAGC AACAGTCTCA CCAGGACCGT GGAAAATTCC TGGCTCTGAT 
AAGCTACCTA GTACCCTGAA ATATGGCACT TCAGCCTTGA GTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753372.1
CDS	355..4842
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTGFRVDGVGPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIDTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYDEPNSWSSPSTTSPGPEVGVSLDDIIAPLSSSHSALPFDPTHQIHPEPAWDIYREQDIGKPKENAANGYKKKRLGESRERSASPKKVDRSMREENFKKEFQESKQKRAENSKTTSVGKVTVKRVLIEEDCGYSTTGDSGGRTSWSAETKSEGTLCAKDVHKDATSEKKSVSGLEQSKSDPSVISKVYSSNITSSRASAMDQDERDAAAKCNDLQKEGPLHSTNTRSVSTAARKATVSPGPWKIPGSDKLPSTLKYGTSALSR

Target ORF information:

RefSeq Version	XM_031897512.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897512.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGATACATG TCCAGAAAAT GGCAGGAGTC CTGGCTCTGC CTCAACACAA 
CACAGTTCTC CCCGAAGTGA TTCTGCTTAT CCAAACAGCA ACCATTCTGC ACCATGCAAT 
CCTTCTCCAC CAGAGGGTTT CACGTCACAA AGCATGCAGT CCAGTGATGT GGTTATACAT 
CGAAAGGAGA ATGAAGGATT TGGTTTTGTC ATAATAAGTT CTTTGAACAG ACCAGAATCA 
GGCGCAACAA TTGCTGTGCC ACATAAAATC GGACGAATTA TTGAAGGAAG CCCTGCAGAT 
CGCTGTGGAA AGCTAAAAGT CGGAGACCGG ATCTTAGCTG TGAATAGCCA ATCTATTATC 
AACATGCCTC ATGCAGATAT TGTAAAGCTG ATTAAGGATG CAGGGCTGAG TGTAACACTT 
TGTATCGTTC CTCAGGAGGA ATTAAACAGT CCAGCTTCAG CACCAAGCTC AGAAAAACAG 
AGTCCTATGG CACAGCAGCA CAGCCCAATG GCACAGCAGA GCCCCGTAGC TCAGCAAAGT 
CCTGTGGCAC ACTCACTGCC CCCTCAACCA CTACAGTTGC AAGGCCATGA GAGCAGCTAT 
AGGTCAGAAG TAAAAGCAAG GCAAGATGTA AAACCAGACA TCAGGCAACC ACCATTTACA 
GATTATCGCC AGCCATCAAC GGACTATAGG CAGCCTCCTG TAGATTACAG ACATCCTCCA 
GTAGTGGATT ACCAGCAGCC TCCTCCTATA GACTACAGAC AGCCACCTTT AATAGAGTAC 
AGGCAACATT CTCCCGATAC TCGGCAATTT CCTCTCCCTG ATTACCGACA ATTTCAGGAT 
TATGATTATT TTACTGTTGA ACTGGAAAAG GGTGCTAAGG GTTTTGGATT TAGTATACGA 
GGAGGAAGAG AATACAAGAT GGATTTGTAT GTGTTAAGGC TGGCAGAAGA TGGCCCTGCA 
ATAAGAAATG GACGAATGAG GGTTGGTGAT CAAATCATAG AAATCAATGG GGAAAGCACA 
AGAGACATGA CACATGCCAG AGCAATAGAA CTCATTAAAT CAGGAGGAAG AAGAGTAAAG 
CTTTTACTGA AGCGTGGAAC AGGTCATGTT CCTGAGTATG ACGAACCAAA CTCCTGGAGT 
TCTCCCTCTA CCACCTCCCC AGGGCCGGAA GTAGGTGTTT CCCTTGATGA CATAATTGCA 
CCATTATCTT CATCACATTC AGCCTTGCCC TTTGACCCTA CTCACCAGAT ACACCCAGAG 
CCAGCATGGG ATATCTACAG GGAACAAGAT ATTGGAAAAC CAAAGGAGAA TGCTGCAAAT 
GGTTATAAAA AGAAAAGACT GGGAGAGTCG AGGGAGAGGT CAGCAAGCCC TAAGAAAGTA 
GACCGATCAA TGCGTGAAGA GAATTTCAAA AAGGAATTTC AGGAAAGCAA ACAGAAAAGA 
GCAGAAAATA GCAAAACCAC CTCAGTGGGC AAAGTGACTG TAAAAAGGGT GTTAATCGAA 
GAAGATTGTG GATATTCTAC AACCGGTGAT AGTGGAGGTC GGACATCCTG GTCCGCTGAA 
ACAAAAAGCG AAGGAACTTT ATGTGCAAAA GACGTACACA AAGATGCAAC CAGTGAGAAA 
AAGTCAGTGT CAGGTCTTGA GCAAAGCAAG TCAGATCCTA GTGTCATCTC CAAGGTGTAC 
AGTAGCAATA TAACCAGCTC TCGAGCGAGT GCCATGGACC AAGATGAGAG AGATGCTGCA 
GCTAAATGCA ATGACCTTCA AAAAGAAGGG CCCCTCCATT CCACCAACAC TCGCAGTGTC 
AGCACTGCAG CTCGGAAAGC AACAGTCTCA CCAGGACCGT GGAAAATTCC TGGCTCTGAT 
AAGCTACCTA GTACCCTGAA ATATGGCACT TCAGCCTTGA GTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( XM_031897513.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753373.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X3, mRNA.

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CloneID	OXa53139
Clone ID Related Accession (Same CDS sequence)	XM_031897513.1
Accession Version	XM_031897513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA AGCAAGTGCC GCCCAGGACT 
GGTTTCAGAG TGGATGGAGT TGGCCCTGAC TATAAGGAGT TGGATGTTCA TCTTCGGCGC 
CAAGAATCTG GATTTGGGTT CAGAATCCTT GGAGGAGATG AACCTGGCCA GCCTATTTTA 
ATTGGAGCTG TAATAGCGAT GGGCTCTGCA GACAGAGATG GACGCCTACG CCCTGGCGAT 
GAGTTGTTAT ACGTAGATGG AATCCCAGTT GCTGGAAAAA CCCACCGATA TGTGATTGAT 
CTTATGCATA ATGCTGCTCG GAATGGACAG GTCAACCTTA CTGTGAGACG AAAGGTGCTA 
TCTATAGCAG ATACATGTCC AGAAAATGGC AGGAGTCCTG GCTCTGCCTC AACACAACAC 
AGTTCTCCCC GAAGTGATTC TGCTTATCCA AACAGCAACC ATTCTGCACC ATGCAATCCT 
TCTCCACCAG AGGGTTTCAC GTCACAAAGC ATGCAGTCCA GTGATGTGGT TATACATCGA 
AAGGAGAATG AAGGATTTGG TTTTGTCATA ATAAGTTCTT TGAACAGACC AGAATCAGGC 
GCAACAATTG CTGTGCCACA TAAAATCGGA CGAATTATTG AAGGAAGCCC TGCAGATCGC 
TGTGGAAAGC TAAAAGTCGG AGACCGGATC TTAGCTGTGA ATAGCCAATC TATTATCAAC 
ATGCCTCATG CAGATATTGT AAAGCTGATT AAGGATGCAG GGCTGAGTGT AACACTTTGT 
ATCGTTCCTC AGGAGGAATT AAACAGTCCA GCTTCAGCAC CAAGCTCAGA AAAACAGAGT 
CCTATGGCAC AGCAGCACAG CCCAATGGCA CAGCAGAGCC CCGTAGCTCA GCAAAGTCCT 
GTGGCACACT CACTGCCCCC TCAACCACTA CAGTTGCAAG GCCATGAGAG CAGCTATAGG 
TCAGAAGTAA AAGCAAGGCA AGATGTAAAA CCAGACATCA GGCAACCACC ATTTACAGAT 
TATCGCCAGC CATCAACGGA CTATAGGCAG CCTCCTGTAG ATTACAGACA TCCTCCAGTA 
GTGGATTACC AGCAGCCTCC TCCTATAGAC TACAGACAGC CACCTTTAAT AGAGTACAGG 
CAACATTCTC CCGATACTCG GCAATTTCCT CTCCCTGATT ACCGACAATT TCAGGATTAT 
GATTATTTTA CTGTTGAACT GGAAAAGGGT GCTAAGGGTT TTGGATTTAG TATACGAGGA 
GGAAGAGAAT ACAAGATGGA TTTGTATGTG TTAAGGCTGG CAGAAGATGG CCCTGCAATA 
AGAAATGGAC GAATGAGGGT TGGTGATCAA ATCATAGAAA TCAATGGGGA AAGCACAAGA 
GACATGACAC ATGCCAGAGC AATAGAACTC ATTAAATCAG GAGGAAGAAG AGTAAAGCTT 
TTACTGAAGC GTGGAACAGG TCATGTTCCT GAGTATGACG AACCAAACTC CTGGAGTTCT 
CCCTCTACCA CCTCCCCAGG GCCGGAAGTA GGTGTTTCCC TTGATGACAT AATTGCACCA 
TTATCTTCAT CACATTCAGC CTTGCCCTTT GACCCTACTC ACCAGATACA CCCAGAGCCA 
GCATGGGATA TCTACAGGGA ACAAGATATT GGAAAACCAA AGGAGAATGC TGCAAATGGT 
TATAAAAAGA AAAGACTGGG AGAGTCGAGG GAGAGGTCAG CAAGCCCTAA GAAAGTAGAC 
CGATCAATGC GTGAAGAGAA TTTCAAAAAG GAATTTCAGG AAAGCAAACA GAAAAGAGCA 
GAAAATAGCA AAACCACCTC AGTGGGCAAA GTGACTGTAA AAAGGGTGTT AATCGAAGAA 
GATTGTGGAT ATTCTACAAC CGGTGATAGT GGAGGTCGGA CATCCTGGTC CGCTGAAACA 
AAAAGCGAAG GAACTTTATG TGCAAAAGAC GTACACAAAG ATGCAACCAG TGAGAAAAAG 
TCAGTGTCAG GTCTTGAGCA AAGCAAGTCA GATCCTAGTG TCATCTCCAA GGTGTACAGT 
AGCAATATAA CCAGCTCTCG AGCGAGTGCC ATGGACCAAG ATGAGAGAGA TGCTGCAGCT 
AAATGCAATG ACCTTCAAAA AGAAGGGCCC CTCCATTCCA CCAACACTCG CAGTGTCAGC 
ACTGCAGCTC GGAAAGCAAC AGTCTCACCA GGACCGTGGA AAATTCCTGG CTCTGATAAG 
CTACCTAGTA CCCTGAAATA TGGCACTTCA GCCTTGAGTA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753373.1
CDS	355..4839
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYEKQVPPRTGFRVDGVGPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIADTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYDEPNSWSSPSTTSPGPEVGVSLDDIIAPLSSSHSALPFDPTHQIHPEPAWDIYREQDIGKPKENAANGYKKKRLGESRERSASPKKVDRSMREENFKKEFQESKQKRAENSKTTSVGKVTVKRVLIEEDCGYSTTGDSGGRTSWSAETKSEGTLCAKDVHKDATSEKKSVSGLEQSKSDPSVISKVYSSNITSSRASAMDQDERDAAAKCNDLQKEGPLHSTNTRSVSTAARKATVSPGPWKIPGSDKLPSTLKYGTSALSR

Target ORF information:

RefSeq Version	XM_031897513.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897513.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA AGCAAGTGCC GCCCAGGACT 
GGTTTCAGAG TGGATGGAGT TGGCCCTGAC TATAAGGAGT TGGATGTTCA TCTTCGGCGC 
CAAGAATCTG GATTTGGGTT CAGAATCCTT GGAGGAGATG AACCTGGCCA GCCTATTTTA 
ATTGGAGCTG TAATAGCGAT GGGCTCTGCA GACAGAGATG GACGCCTACG CCCTGGCGAT 
GAGTTGTTAT ACGTAGATGG AATCCCAGTT GCTGGAAAAA CCCACCGATA TGTGATTGAT 
CTTATGCATA ATGCTGCTCG GAATGGACAG GTCAACCTTA CTGTGAGACG AAAGGTGCTA 
TCTATAGCAG ATACATGTCC AGAAAATGGC AGGAGTCCTG GCTCTGCCTC AACACAACAC 
AGTTCTCCCC GAAGTGATTC TGCTTATCCA AACAGCAACC ATTCTGCACC ATGCAATCCT 
TCTCCACCAG AGGGTTTCAC GTCACAAAGC ATGCAGTCCA GTGATGTGGT TATACATCGA 
AAGGAGAATG AAGGATTTGG TTTTGTCATA ATAAGTTCTT TGAACAGACC AGAATCAGGC 
GCAACAATTG CTGTGCCACA TAAAATCGGA CGAATTATTG AAGGAAGCCC TGCAGATCGC 
TGTGGAAAGC TAAAAGTCGG AGACCGGATC TTAGCTGTGA ATAGCCAATC TATTATCAAC 
ATGCCTCATG CAGATATTGT AAAGCTGATT AAGGATGCAG GGCTGAGTGT AACACTTTGT 
ATCGTTCCTC AGGAGGAATT AAACAGTCCA GCTTCAGCAC CAAGCTCAGA AAAACAGAGT 
CCTATGGCAC AGCAGCACAG CCCAATGGCA CAGCAGAGCC CCGTAGCTCA GCAAAGTCCT 
GTGGCACACT CACTGCCCCC TCAACCACTA CAGTTGCAAG GCCATGAGAG CAGCTATAGG 
TCAGAAGTAA AAGCAAGGCA AGATGTAAAA CCAGACATCA GGCAACCACC ATTTACAGAT 
TATCGCCAGC CATCAACGGA CTATAGGCAG CCTCCTGTAG ATTACAGACA TCCTCCAGTA 
GTGGATTACC AGCAGCCTCC TCCTATAGAC TACAGACAGC CACCTTTAAT AGAGTACAGG 
CAACATTCTC CCGATACTCG GCAATTTCCT CTCCCTGATT ACCGACAATT TCAGGATTAT 
GATTATTTTA CTGTTGAACT GGAAAAGGGT GCTAAGGGTT TTGGATTTAG TATACGAGGA 
GGAAGAGAAT ACAAGATGGA TTTGTATGTG TTAAGGCTGG CAGAAGATGG CCCTGCAATA 
AGAAATGGAC GAATGAGGGT TGGTGATCAA ATCATAGAAA TCAATGGGGA AAGCACAAGA 
GACATGACAC ATGCCAGAGC AATAGAACTC ATTAAATCAG GAGGAAGAAG AGTAAAGCTT 
TTACTGAAGC GTGGAACAGG TCATGTTCCT GAGTATGACG AACCAAACTC CTGGAGTTCT 
CCCTCTACCA CCTCCCCAGG GCCGGAAGTA GGTGTTTCCC TTGATGACAT AATTGCACCA 
TTATCTTCAT CACATTCAGC CTTGCCCTTT GACCCTACTC ACCAGATACA CCCAGAGCCA 
GCATGGGATA TCTACAGGGA ACAAGATATT GGAAAACCAA AGGAGAATGC TGCAAATGGT 
TATAAAAAGA AAAGACTGGG AGAGTCGAGG GAGAGGTCAG CAAGCCCTAA GAAAGTAGAC 
CGATCAATGC GTGAAGAGAA TTTCAAAAAG GAATTTCAGG AAAGCAAACA GAAAAGAGCA 
GAAAATAGCA AAACCACCTC AGTGGGCAAA GTGACTGTAA AAAGGGTGTT AATCGAAGAA 
GATTGTGGAT ATTCTACAAC CGGTGATAGT GGAGGTCGGA CATCCTGGTC CGCTGAAACA 
AAAAGCGAAG GAACTTTATG TGCAAAAGAC GTACACAAAG ATGCAACCAG TGAGAAAAAG 
TCAGTGTCAG GTCTTGAGCA AAGCAAGTCA GATCCTAGTG TCATCTCCAA GGTGTACAGT 
AGCAATATAA CCAGCTCTCG AGCGAGTGCC ATGGACCAAG ATGAGAGAGA TGCTGCAGCT 
AAATGCAATG ACCTTCAAAA AGAAGGGCCC CTCCATTCCA CCAACACTCG CAGTGTCAGC 
ACTGCAGCTC GGAAAGCAAC AGTCTCACCA GGACCGTGGA AAATTCCTGG CTCTGATAAG 
CTACCTAGTA CCCTGAAATA TGGCACTTCA GCCTTGAGTA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( XM_031897514.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753374.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X4, mRNA.

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CloneID	OXa53140
Clone ID Related Accession (Same CDS sequence)	XM_031897514.1
Accession Version	XM_031897514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCGCCC TGACTATAAG 
GAGTTGGATG TTCATCTTCG GCGCCAAGAA TCTGGATTTG GGTTCAGAAT CCTTGGAGGA 
GATGAACCTG GCCAGCCTAT TTTAATTGGA GCTGTAATAG CGATGGGCTC TGCAGACAGA 
GATGGACGCC TACGCCCTGG CGATGAGTTG TTATACGTAG ATGGAATCCC AGTTGCTGGA 
AAAACCCACC GATATGTGAT TGATCTTATG CATAATGCTG CTCGGAATGG ACAGGTCAAC 
CTTACTGTGA GACGAAAGGT GCTATCTATA GCAGATACAT GTCCAGAAAA TGGCAGGAGT 
CCTGGCTCTG CCTCAACACA ACACAGTTCT CCCCGAAGTG ATTCTGCTTA TCCAAACAGC 
AACCATTCTG CACCATGCAA TCCTTCTCCA CCAGAGGGTT TCACGTCACA AAGCATGCAG 
TCCAGTGATG TGGTTATACA TCGAAAGGAG AATGAAGGAT TTGGTTTTGT CATAATAAGT 
TCTTTGAACA GACCAGAATC AGGCGCAACA ATTGCTGTGC CACATAAAAT CGGACGAATT 
ATTGAAGGAA GCCCTGCAGA TCGCTGTGGA AAGCTAAAAG TCGGAGACCG GATCTTAGCT 
GTGAATAGCC AATCTATTAT CAACATGCCT CATGCAGATA TTGTAAAGCT GATTAAGGAT 
GCAGGGCTGA GTGTAACACT TTGTATCGTT CCTCAGGAGG AATTAAACAG TCCAGCTTCA 
GCACCAAGCT CAGAAAAACA GAGTCCTATG GCACAGCAGC ACAGCCCAAT GGCACAGCAG 
AGCCCCGTAG CTCAGCAAAG TCCTGTGGCA CACTCACTGC CCCCTCAACC ACTACAGTTG 
CAAGGCCATG AGAGCAGCTA TAGGTCAGAA GTAAAAGCAA GGCAAGATGT AAAACCAGAC 
ATCAGGCAAC CACCATTTAC AGATTATCGC CAGCCATCAA CGGACTATAG GCAGCCTCCT 
GTAGATTACA GACATCCTCC AGTAGTGGAT TACCAGCAGC CTCCTCCTAT AGACTACAGA 
CAGCCACCTT TAATAGAGTA CAGGCAACAT TCTCCCGATA CTCGGCAATT TCCTCTCCCT 
GATTACCGAC AATTTCAGGA TTATGATTAT TTTACTGTTG AACTGGAAAA GGGTGCTAAG 
GGTTTTGGAT TTAGTATACG AGGAGGAAGA GAATACAAGA TGGATTTGTA TGTGTTAAGG 
CTGGCAGAAG ATGGCCCTGC AATAAGAAAT GGACGAATGA GGGTTGGTGA TCAAATCATA 
GAAATCAATG GGGAAAGCAC AAGAGACATG ACACATGCCA GAGCAATAGA ACTCATTAAA 
TCAGGAGGAA GAAGAGTAAA GCTTTTACTG AAGCGTGGAA CAGGTCATGT TCCTGAGTAT 
GACGAACCAA ACTCCTGGAG TTCTCCCTCT ACCACCTCCC CAGGGCCGGA AGTAGGTGTT 
TCCCTTGATG ACATAATTGC ACCATTATCT TCATCACATT CAGCCTTGCC CTTTGACCCT 
ACTCACCAGA TACACCCAGA GCCAGCATGG GATATCTACA GGGAACAAGA TATTGGAAAA 
CCAAAGGAGA ATGCTGCAAA TGGTTATAAA AAGAAAAGAC TGGGAGAGTC GAGGGAGAGG 
TCAGCAAGCC CTAAGAAAGT AGACCGATCA ATGCGTGAAG AGAATTTCAA AAAGGAATTT 
CAGGAAAGCA AACAGAAAAG AGCAGAAAAT AGCAAAACCA CCTCAGTGGG CAAAGTGACT 
GTAAAAAGGG TGTTAATCGA AGAAGATTGT GGATATTCTA CAACCGGTGA TAGTGGAGGT 
CGGACATCCT GGTCCGCTGA AACAAAAAGC GAAGGAACTT TATGTGCAAA AGACGTACAC 
AAAGATGCAA CCAGTGAGAA AAAGTCAGTG TCAGGTCTTG AGCAAAGCAA GTCAGATCCT 
AGTGTCATCT CCAAGGTGTA CAGTAGCAAT ATAACCAGCT CTCGAGCGAG TGCCATGGAC 
CAAGATGAGA GAGATGCTGC AGCTAAATGC AATGACCTTC AAAAAGAAGG GCCCCTCCAT 
TCCACCAACA CTCGCAGTGT CAGCACTGCA GCTCGGAAAG CAACAGTCTC ACCAGGACCG 
TGGAAAATTC CTGGCTCTGA TAAGCTACCT AGTACCCTGA AATATGGCAC TTCAGCCTTG 
AGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753374.1
CDS	354..4802
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRRPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIADTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYDEPNSWSSPSTTSPGPEVGVSLDDIIAPLSSSHSALPFDPTHQIHPEPAWDIYREQDIGKPKENAANGYKKKRLGESRERSASPKKVDRSMREENFKKEFQESKQKRAENSKTTSVGKVTVKRVLIEEDCGYSTTGDSGGRTSWSAETKSEGTLCAKDVHKDATSEKKSVSGLEQSKSDPSVISKVYSSNITSSRASAMDQDERDAAAKCNDLQKEGPLHSTNTRSVSTAARKATVSPGPWKIPGSDKLPSTLKYGTSALSR

Target ORF information:

RefSeq Version	XM_031897514.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897514.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCGCCC TGACTATAAG 
GAGTTGGATG TTCATCTTCG GCGCCAAGAA TCTGGATTTG GGTTCAGAAT CCTTGGAGGA 
GATGAACCTG GCCAGCCTAT TTTAATTGGA GCTGTAATAG CGATGGGCTC TGCAGACAGA 
GATGGACGCC TACGCCCTGG CGATGAGTTG TTATACGTAG ATGGAATCCC AGTTGCTGGA 
AAAACCCACC GATATGTGAT TGATCTTATG CATAATGCTG CTCGGAATGG ACAGGTCAAC 
CTTACTGTGA GACGAAAGGT GCTATCTATA GCAGATACAT GTCCAGAAAA TGGCAGGAGT 
CCTGGCTCTG CCTCAACACA ACACAGTTCT CCCCGAAGTG ATTCTGCTTA TCCAAACAGC 
AACCATTCTG CACCATGCAA TCCTTCTCCA CCAGAGGGTT TCACGTCACA AAGCATGCAG 
TCCAGTGATG TGGTTATACA TCGAAAGGAG AATGAAGGAT TTGGTTTTGT CATAATAAGT 
TCTTTGAACA GACCAGAATC AGGCGCAACA ATTGCTGTGC CACATAAAAT CGGACGAATT 
ATTGAAGGAA GCCCTGCAGA TCGCTGTGGA AAGCTAAAAG TCGGAGACCG GATCTTAGCT 
GTGAATAGCC AATCTATTAT CAACATGCCT CATGCAGATA TTGTAAAGCT GATTAAGGAT 
GCAGGGCTGA GTGTAACACT TTGTATCGTT CCTCAGGAGG AATTAAACAG TCCAGCTTCA 
GCACCAAGCT CAGAAAAACA GAGTCCTATG GCACAGCAGC ACAGCCCAAT GGCACAGCAG 
AGCCCCGTAG CTCAGCAAAG TCCTGTGGCA CACTCACTGC CCCCTCAACC ACTACAGTTG 
CAAGGCCATG AGAGCAGCTA TAGGTCAGAA GTAAAAGCAA GGCAAGATGT AAAACCAGAC 
ATCAGGCAAC CACCATTTAC AGATTATCGC CAGCCATCAA CGGACTATAG GCAGCCTCCT 
GTAGATTACA GACATCCTCC AGTAGTGGAT TACCAGCAGC CTCCTCCTAT AGACTACAGA 
CAGCCACCTT TAATAGAGTA CAGGCAACAT TCTCCCGATA CTCGGCAATT TCCTCTCCCT 
GATTACCGAC AATTTCAGGA TTATGATTAT TTTACTGTTG AACTGGAAAA GGGTGCTAAG 
GGTTTTGGAT TTAGTATACG AGGAGGAAGA GAATACAAGA TGGATTTGTA TGTGTTAAGG 
CTGGCAGAAG ATGGCCCTGC AATAAGAAAT GGACGAATGA GGGTTGGTGA TCAAATCATA 
GAAATCAATG GGGAAAGCAC AAGAGACATG ACACATGCCA GAGCAATAGA ACTCATTAAA 
TCAGGAGGAA GAAGAGTAAA GCTTTTACTG AAGCGTGGAA CAGGTCATGT TCCTGAGTAT 
GACGAACCAA ACTCCTGGAG TTCTCCCTCT ACCACCTCCC CAGGGCCGGA AGTAGGTGTT 
TCCCTTGATG ACATAATTGC ACCATTATCT TCATCACATT CAGCCTTGCC CTTTGACCCT 
ACTCACCAGA TACACCCAGA GCCAGCATGG GATATCTACA GGGAACAAGA TATTGGAAAA 
CCAAAGGAGA ATGCTGCAAA TGGTTATAAA AAGAAAAGAC TGGGAGAGTC GAGGGAGAGG 
TCAGCAAGCC CTAAGAAAGT AGACCGATCA ATGCGTGAAG AGAATTTCAA AAAGGAATTT 
CAGGAAAGCA AACAGAAAAG AGCAGAAAAT AGCAAAACCA CCTCAGTGGG CAAAGTGACT 
GTAAAAAGGG TGTTAATCGA AGAAGATTGT GGATATTCTA CAACCGGTGA TAGTGGAGGT 
CGGACATCCT GGTCCGCTGA AACAAAAAGC GAAGGAACTT TATGTGCAAA AGACGTACAC 
AAAGATGCAA CCAGTGAGAA AAAGTCAGTG TCAGGTCTTG AGCAAAGCAA GTCAGATCCT 
AGTGTCATCT CCAAGGTGTA CAGTAGCAAT ATAACCAGCT CTCGAGCGAG TGCCATGGAC 
CAAGATGAGA GAGATGCTGC AGCTAAATGC AATGACCTTC AAAAAGAAGG GCCCCTCCAT 
TCCACCAACA CTCGCAGTGT CAGCACTGCA GCTCGGAAAG CAACAGTCTC ACCAGGACCG 
TGGAAAATTC CTGGCTCTGA TAAGCTACCT AGTACCCTGA AATATGGCAC TTCAGCCTTG 
AGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( XM_031897515.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753375.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa53141
Clone ID Related Accession (Same CDS sequence)	XM_031897515.1
Accession Version	XM_031897515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG AGAAAAGTGG AGCTCTCTTG GAAAGTGGAA CTTATGAAGA AAATTACTAC 
GGAACTCCAA AGCCTCCTGC TGAACCTGCT CCACTTTTAT TAAATGCAAC AGACCATATT 
TTTCCTGGTG GCATTCCAAG AGTTGAGGGA AAACGTAAAC GCAATAAGTC TGTAAGCAAC 
ATGGAGAAAA CAGGGATTGA GCCTCCAGAA GAAGAGGAAG AAGAGAGGCC TGTGGTAAAT 
GGAAATGGTG TTGCAGTGAC CCCAGAATCA AGTGAACATG AAGATAAGAG TACAGATGCC 
TCAGGTGAGA TCTCTTCCCT GCCTTACACT GCTCCGCTGT ACTGCCATTC AGAAGAAAAG 
GAAGGCACAG ACACAATCAA GATCCTAAAG CCTGATGACG CTGATGAGCT GGGGTCTTTG 
CCTGATAACT GGGAAATGGC ATATACAGAA AAAGGAGAAG TCTACTTTAT TGACCACAAC 
ACAAAGACAA CGTCATGGCT GGATCCACGG TTAGCAAAAA AAGCAAAGCC TCCAGAAGAG 
TGCAAAGAGA ATGAGTTACC TTATGGCTGG GAGAAAATCG ATGATCCAAT ATATGGCACG 
TATTATGTTG ATCACATAAA CAGAAGAACT CAGTTTGAAA ACCCAGTATT AGAAGCAAAA 
AGAAAGCTTC AGCAGCACAG TATGCCCTCA ACAGAACTGG GAACCCAGCC GATGCAGCCC 
CAAGTATTTA GAGAAAAGCC ACTGTTTACC CGTGATGCAT CACAACTAAA GGGAACTTTT 
CTAAGTACCA TACTGAAGAA GAGTAATATG GGTTTTGGAT TTACCATCAT TGGAGGAGAT 
GAACCAGATG AGTTTCTGCA GGTAAAGAGT GTAATCCCTG AAGGCCCAGC TGCACAGGAT 
GGTAAAATGG AGACAGGTGA TGTAATTGTC TATATAAATG AAGCGTGTGT GCTTGGGTAC 
ACACATGCGG ATGTGGTCAA AATTTTCCAG TCTGTTCCCA TTGGGCAAAG TGTCAACTTG 
GTGCTATGTC GAGGATATCC CCTACCCTAC GACCCAGAAG ATTCTGCAAA CAACTTGGTA 
TCACCTTTAG CAGTCATTGA AAGGCCGCCA GTGGTTGTAA ATGGAAGAAA TAACTATGAA 
ACTTATTTGG AATACATTTC TAGGACGTCA CAATCAGTAC CAGATGTAGC AGAACGACCA 
TTGCCTTCTT TGCACTCTGT TCCAACAGAC AGTCCGCTTG AAAATACATT TCCACCGCCT 
CCACCTACAC ATGATGATAA TGTATCAATG GCTTCTTCTG GAGCCACACA AGCAGAACTT 
ATGACCTTAA CCATTGTGAA AGGGGCACAG GGCTTTGGCT TCACAATAGC AGACAGTCCA 
ACAGGACAGC GAGTGAAGCA GATTTTAGAT GTTCAGGGAT GCCCTGGTTT GTTTGAAGGG 
GACCTTATAG TTGAGATCAA CCAGCAGAAT GTACAGAATT TGAACCACTT AGAAGTTGTG 
GAGTTACTTA AAGAATGTCC TGTTGGAAAT GAGACTTCTT TGGTGATCCA GAGAGGAGGT 
TTCTTTTCTC CGTGGAAAAG TTCCAAGCCT ATAATGGAAA GATGGGAAAA CCAAGGAAGC 
CCTCAGACTA GTTTATCAGC ACCTTCCTTA CCACCAAATG TCCCTTTTGC TCCAATGCTA 
CATAGGAGTT CATTTCCCGA TTCCACAGAG GCCTTTGACC CTAGAAAACC TGACCCCTAT 
GAACTATATG AAAAATCAAG AGCAATTTAT GAAAGTAGGC AGCAAGTGCC GCCCAGGACT 
GGTTTCAGAG TGGATGGAGT TGGCCCTGAC TATAAGGAGT TGGATGTTCA TCTTCGGCGC 
CAAGAATCTG GATTTGGGTT CAGAATCCTT GGAGGAGATG AACCTGGCCA GCCTATTTTA 
ATTGGAGCTG TAATAGCGAT GGGCTCTGCA GACAGAGATG GACGCCTACG CCCTGGCGAT 
GAGTTGTTAT ACGTAGATGG AATCCCAGTT GCTGGAAAAA CCCACCGATA TGTGATTGAT 
CTTATGCATA ATGCTGCTCG GAATGGACAG GTCAACCTTA CTGTGAGACG AAAGGTGCTA 
TCTATAGCAG ATACATGTCC AGAAAATGGC AGGAGTCCTG GCTCTGCCTC AACACAACAC 
AGTTCTCCCC GAAGTGATTC TGCTTATCCA AACAGCAACC ATTCTGCACC ATGCAATCCT 
TCTCCACCAG AGGGTTTCAC GTCACAAAGC ATGCAGTCCA GTGATGTGGT TATACATCGA 
AAGGAGAATG AAGGATTTGG TTTTGTCATA ATAAGTTCTT TGAACAGACC AGAATCAGGC 
GCAACAATTG CTGTGCCACA TAAAATCGGA CGAATTATTG AAGGAAGCCC TGCAGATCGC 
TGTGGAAAGC TAAAAGTCGG AGACCGGATC TTAGCTGTGA ATAGCCAATC TATTATCAAC 
ATGCCTCATG CAGATATTGT AAAGCTGATT AAGGATGCAG GGCTGAGTGT AACACTTTGT 
ATCGTTCCTC AGGAGGAATT AAACAGTCCA GCTTCAGCAC CAAGCTCAGA AAAACAGAGT 
CCTATGGCAC AGCAGCACAG CCCAATGGCA CAGCAGAGCC CCGTAGCTCA GCAAAGTCCT 
GTGGCACACT CACTGCCCCC TCAACCACTA CAGTTGCAAG GCCATGAGAG CAGCTATAGG 
TCAGAAGTAA AAGCAAGGCA AGATGTAAAA CCAGACATCA GGCAACCACC ATTTACAGAT 
TATCGCCAGC CATCAACGGA CTATAGGCAG CCTCCTGTAG ATTACAGACA TCCTCCAGTA 
GTGGATTACC AGCAGCCTCC TCCTATAGAC TACAGACAGC CACCTTTAAT AGAGTACAGG 
CAACATTCTC CCGATACTCG GCAATTTCCT CTCCCTGATT ACCGACAATT TCAGGATTAT 
GATTATTTTA CTGTTGAACT GGAAAAGGGT GCTAAGGGTT TTGGATTTAG TATACGAGGA 
GGAAGAGAAT ACAAGATGGA TTTGTATGTG TTAAGGCTGG CAGAAGATGG CCCTGCAATA 
AGAAATGGAC GAATGAGGGT TGGTGATCAA ATCATAGAAA TCAATGGGGA AAGCACAAGA 
GACATGACAC ATGCCAGAGC AATAGAACTC ATTAAATCAG GAGGAAGAAG AGTAAAGCTT 
TTACTGAAGC GTGGAACAGG TCATGTTCCT GAGTATGACG AACCAAACTC CTGGAGTTCT 
CCCTCTACCA CCTCCCCAGG GCCGGAAGTA GGTGTTTCCC TTGATGACAT AATTGCACCA 
TTATCTTCAT CACATTCAGC CTTGCCCTTT GACCCTACTC ACCAGATACA CCCAGAGCCA 
GCATGGGATA TCTACAGGGA ACAAGATATT GGAAAACCAA AGGAGAATGC TGCAAATGGT 
TATAAAAAGA AAAGACTGGG AGAGTCGAGG GAGAGGTCAG CAAGCCCTAA GAAAGTAGAC 
CGATCAATGC GTGAAGAGAA TTTCAAAAAG GAATTTCAGG AAAGCAAACA GAAAAGAGCA 
GAAAATAGCA AAACCACCTC AGTGGGCAAA GTGACTGTAA AAAGGGTGTT AATCGAAGAA 
GATTGTGGAT ATTCTACAAC CGGTGATAGT GGAGGTCGGA CATCCTGGTC CGCTGAAACA 
AAAAGCGAAG GAACTTTATG TGCAAAAGAC GTACACAAAG ATGCAACCAG TGAGAAAAAG 
TCAGTGTCAG GTCTTGAGCA AAGCAAGTCA GATCCTAGTG TCATCTCCAA GGTGTACAGT 
AGCAATATAA CCAGCTCTCG AGCGAGTGCC ATGGACCAAG ATGAGAGAGA TGCTGCAGCT 
AAATGCAATG ACCTTCAAAA AGAAGGGCCC CTCCATTCCA CCAACACTCG CAGTGTCAGC 
ACTGCAGCTC GGAAAGCAAC AGTCTCACCA GGACCGTGGA AAATTCCTGG CTCTGATAAG 
CTACCTAGTA CCCTGAAATA TGGCACTTCA GCCTTGAGTA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753375.1
CDS	324..4328
Translation	MELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTGFRVDGVGPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIADTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYDEPNSWSSPSTTSPGPEVGVSLDDIIAPLSSSHSALPFDPTHQIHPEPAWDIYREQDIGKPKENAANGYKKKRLGESRERSASPKKVDRSMREENFKKEFQESKQKRAENSKTTSVGKVTVKRVLIEEDCGYSTTGDSGGRTSWSAETKSEGTLCAKDVHKDATSEKKSVSGLEQSKSDPSVISKVYSSNITSSRASAMDQDERDAAAKCNDLQKEGPLHSTNTRSVSTAARKATVSPGPWKIPGSDKLPSTLKYGTSALSR

Target ORF information:

RefSeq Version	XM_031897515.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897515.1

ORF Insert Sequence:

ATGGAATTAG AGAAAAGTGG AGCTCTCTTG GAAAGTGGAA CTTATGAAGA AAATTACTAC 
GGAACTCCAA AGCCTCCTGC TGAACCTGCT CCACTTTTAT TAAATGCAAC AGACCATATT 
TTTCCTGGTG GCATTCCAAG AGTTGAGGGA AAACGTAAAC GCAATAAGTC TGTAAGCAAC 
ATGGAGAAAA CAGGGATTGA GCCTCCAGAA GAAGAGGAAG AAGAGAGGCC TGTGGTAAAT 
GGAAATGGTG TTGCAGTGAC CCCAGAATCA AGTGAACATG AAGATAAGAG TACAGATGCC 
TCAGGTGAGA TCTCTTCCCT GCCTTACACT GCTCCGCTGT ACTGCCATTC AGAAGAAAAG 
GAAGGCACAG ACACAATCAA GATCCTAAAG CCTGATGACG CTGATGAGCT GGGGTCTTTG 
CCTGATAACT GGGAAATGGC ATATACAGAA AAAGGAGAAG TCTACTTTAT TGACCACAAC 
ACAAAGACAA CGTCATGGCT GGATCCACGG TTAGCAAAAA AAGCAAAGCC TCCAGAAGAG 
TGCAAAGAGA ATGAGTTACC TTATGGCTGG GAGAAAATCG ATGATCCAAT ATATGGCACG 
TATTATGTTG ATCACATAAA CAGAAGAACT CAGTTTGAAA ACCCAGTATT AGAAGCAAAA 
AGAAAGCTTC AGCAGCACAG TATGCCCTCA ACAGAACTGG GAACCCAGCC GATGCAGCCC 
CAAGTATTTA GAGAAAAGCC ACTGTTTACC CGTGATGCAT CACAACTAAA GGGAACTTTT 
CTAAGTACCA TACTGAAGAA GAGTAATATG GGTTTTGGAT TTACCATCAT TGGAGGAGAT 
GAACCAGATG AGTTTCTGCA GGTAAAGAGT GTAATCCCTG AAGGCCCAGC TGCACAGGAT 
GGTAAAATGG AGACAGGTGA TGTAATTGTC TATATAAATG AAGCGTGTGT GCTTGGGTAC 
ACACATGCGG ATGTGGTCAA AATTTTCCAG TCTGTTCCCA TTGGGCAAAG TGTCAACTTG 
GTGCTATGTC GAGGATATCC CCTACCCTAC GACCCAGAAG ATTCTGCAAA CAACTTGGTA 
TCACCTTTAG CAGTCATTGA AAGGCCGCCA GTGGTTGTAA ATGGAAGAAA TAACTATGAA 
ACTTATTTGG AATACATTTC TAGGACGTCA CAATCAGTAC CAGATGTAGC AGAACGACCA 
TTGCCTTCTT TGCACTCTGT TCCAACAGAC AGTCCGCTTG AAAATACATT TCCACCGCCT 
CCACCTACAC ATGATGATAA TGTATCAATG GCTTCTTCTG GAGCCACACA AGCAGAACTT 
ATGACCTTAA CCATTGTGAA AGGGGCACAG GGCTTTGGCT TCACAATAGC AGACAGTCCA 
ACAGGACAGC GAGTGAAGCA GATTTTAGAT GTTCAGGGAT GCCCTGGTTT GTTTGAAGGG 
GACCTTATAG TTGAGATCAA CCAGCAGAAT GTACAGAATT TGAACCACTT AGAAGTTGTG 
GAGTTACTTA AAGAATGTCC TGTTGGAAAT GAGACTTCTT TGGTGATCCA GAGAGGAGGT 
TTCTTTTCTC CGTGGAAAAG TTCCAAGCCT ATAATGGAAA GATGGGAAAA CCAAGGAAGC 
CCTCAGACTA GTTTATCAGC ACCTTCCTTA CCACCAAATG TCCCTTTTGC TCCAATGCTA 
CATAGGAGTT CATTTCCCGA TTCCACAGAG GCCTTTGACC CTAGAAAACC TGACCCCTAT 
GAACTATATG AAAAATCAAG AGCAATTTAT GAAAGTAGGC AGCAAGTGCC GCCCAGGACT 
GGTTTCAGAG TGGATGGAGT TGGCCCTGAC TATAAGGAGT TGGATGTTCA TCTTCGGCGC 
CAAGAATCTG GATTTGGGTT CAGAATCCTT GGAGGAGATG AACCTGGCCA GCCTATTTTA 
ATTGGAGCTG TAATAGCGAT GGGCTCTGCA GACAGAGATG GACGCCTACG CCCTGGCGAT 
GAGTTGTTAT ACGTAGATGG AATCCCAGTT GCTGGAAAAA CCCACCGATA TGTGATTGAT 
CTTATGCATA ATGCTGCTCG GAATGGACAG GTCAACCTTA CTGTGAGACG AAAGGTGCTA 
TCTATAGCAG ATACATGTCC AGAAAATGGC AGGAGTCCTG GCTCTGCCTC AACACAACAC 
AGTTCTCCCC GAAGTGATTC TGCTTATCCA AACAGCAACC ATTCTGCACC ATGCAATCCT 
TCTCCACCAG AGGGTTTCAC GTCACAAAGC ATGCAGTCCA GTGATGTGGT TATACATCGA 
AAGGAGAATG AAGGATTTGG TTTTGTCATA ATAAGTTCTT TGAACAGACC AGAATCAGGC 
GCAACAATTG CTGTGCCACA TAAAATCGGA CGAATTATTG AAGGAAGCCC TGCAGATCGC 
TGTGGAAAGC TAAAAGTCGG AGACCGGATC TTAGCTGTGA ATAGCCAATC TATTATCAAC 
ATGCCTCATG CAGATATTGT AAAGCTGATT AAGGATGCAG GGCTGAGTGT AACACTTTGT 
ATCGTTCCTC AGGAGGAATT AAACAGTCCA GCTTCAGCAC CAAGCTCAGA AAAACAGAGT 
CCTATGGCAC AGCAGCACAG CCCAATGGCA CAGCAGAGCC CCGTAGCTCA GCAAAGTCCT 
GTGGCACACT CACTGCCCCC TCAACCACTA CAGTTGCAAG GCCATGAGAG CAGCTATAGG 
TCAGAAGTAA AAGCAAGGCA AGATGTAAAA CCAGACATCA GGCAACCACC ATTTACAGAT 
TATCGCCAGC CATCAACGGA CTATAGGCAG CCTCCTGTAG ATTACAGACA TCCTCCAGTA 
GTGGATTACC AGCAGCCTCC TCCTATAGAC TACAGACAGC CACCTTTAAT AGAGTACAGG 
CAACATTCTC CCGATACTCG GCAATTTCCT CTCCCTGATT ACCGACAATT TCAGGATTAT 
GATTATTTTA CTGTTGAACT GGAAAAGGGT GCTAAGGGTT TTGGATTTAG TATACGAGGA 
GGAAGAGAAT ACAAGATGGA TTTGTATGTG TTAAGGCTGG CAGAAGATGG CCCTGCAATA 
AGAAATGGAC GAATGAGGGT TGGTGATCAA ATCATAGAAA TCAATGGGGA AAGCACAAGA 
GACATGACAC ATGCCAGAGC AATAGAACTC ATTAAATCAG GAGGAAGAAG AGTAAAGCTT 
TTACTGAAGC GTGGAACAGG TCATGTTCCT GAGTATGACG AACCAAACTC CTGGAGTTCT 
CCCTCTACCA CCTCCCCAGG GCCGGAAGTA GGTGTTTCCC TTGATGACAT AATTGCACCA 
TTATCTTCAT CACATTCAGC CTTGCCCTTT GACCCTACTC ACCAGATACA CCCAGAGCCA 
GCATGGGATA TCTACAGGGA ACAAGATATT GGAAAACCAA AGGAGAATGC TGCAAATGGT 
TATAAAAAGA AAAGACTGGG AGAGTCGAGG GAGAGGTCAG CAAGCCCTAA GAAAGTAGAC 
CGATCAATGC GTGAAGAGAA TTTCAAAAAG GAATTTCAGG AAAGCAAACA GAAAAGAGCA 
GAAAATAGCA AAACCACCTC AGTGGGCAAA GTGACTGTAA AAAGGGTGTT AATCGAAGAA 
GATTGTGGAT ATTCTACAAC CGGTGATAGT GGAGGTCGGA CATCCTGGTC CGCTGAAACA 
AAAAGCGAAG GAACTTTATG TGCAAAAGAC GTACACAAAG ATGCAACCAG TGAGAAAAAG 
TCAGTGTCAG GTCTTGAGCA AAGCAAGTCA GATCCTAGTG TCATCTCCAA GGTGTACAGT 
AGCAATATAA CCAGCTCTCG AGCGAGTGCC ATGGACCAAG ATGAGAGAGA TGCTGCAGCT 
AAATGCAATG ACCTTCAAAA AGAAGGGCCC CTCCATTCCA CCAACACTCG CAGTGTCAGC 
ACTGCAGCTC GGAAAGCAAC AGTCTCACCA GGACCGTGGA AAATTCCTGG CTCTGATAAG 
CTACCTAGTA CCCTGAAATA TGGCACTTCA GCCTTGAGTA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi2 ( XM_031897516.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753376.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa53142
Clone ID Related Accession (Same CDS sequence)	XM_031897516.1
Accession Version	XM_031897516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGCAGATAC ATGTCCAGAA AATGGCAGGA GTCCTGGCTC TGCCTCAACA 
CAACACAGTT CTCCCCGAAG TGATTCTGCT TATCCAAACA GCAACCATTC TGCACCATGC 
AATCCTTCTC CACCAGAGGG TTTCACGTCA CAAAGCATGC AGTCCAGTGA TGTGGTTATA 
CATCGAAAGG AGAATGAAGG ATTTGGTTTT GTCATAATAA GTTCTTTGAA CAGACCAGAA 
TCAGGCGCAA CAATTGCTGT GCCACATAAA ATCGGACGAA TTATTGAAGG AAGCCCTGCA 
GATCGCTGTG GAAAGCTAAA AGTCGGAGAC CGGATCTTAG CTGTGAATAG CCAATCTATT 
ATCAACATGC CTCATGCAGA TATTGTAAAG CTGATTAAGG ATGCAGGGCT GAGTGTAACA 
CTTTGTATCG TTCCTCAGGA GGAATTAAAC AGTCCAGCTT CAGCACCAAG CTCAGAAAAA 
CAGAGTCCTA TGGCACAGCA GCACAGCCCA ATGGCACAGC AGAGCCCCGT AGCTCAGCAA 
AGTCCTGTGG CACACTCACT GCCCCCTCAA CCACTACAGT TGCAAGGCCA TGAGAGCAGC 
TATAGGTCAG AAGTAAAAGC AAGGCAAGAT GTAAAACCAG ACATCAGGCA ACCACCATTT 
ACAGATTATC GCCAGCCATC AACGGACTAT AGGCAGCCTC CTGTAGATTA CAGACATCCT 
CCAGTAGTGG ATTACCAGCA GCCTCCTCCT ATAGACTACA GACAGCCACC TTTAATAGAG 
TACAGGCAAC ATTCTCCCGA TACTCGGCAA TTTCCTCTCC CTGATTACCG ACAATTTCAG 
GATTATGATT ATTTTACTGT TGAACTGGAA AAGGGTGCTA AGGGTTTTGG ATTTAGTATA 
CGAGGAGGAA GAGAATACAA GATGGATTTG TATGTGTTAA GGCTGGCAGA AGATGGCCCT 
GCAATAAGAA ATGGACGAAT GAGGGTTGGT GATCAAATCA TAGAAATCAA TGGGGAAAGC 
ACAAGAGACA TGACACATGC CAGAGCAATA GAACTCATTA AATCAGGAGG AAGAAGAGTA 
AAGCTTTTAC TGAAGCGTGG AACAGGTCAT GTTCCTGAGT ATGGCTGTTC CTCCCCTTCC 
TTTAGGAATG GTTCCTTCTT CCAGTCTCTC CATGTGCATG AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753376.1
CDS	359..4126
Translation	MSKSLKRKNHWTNKVHECVIERGQEGELGFEIRGGAESGHFPYLGEVKPGKVSYQSGKLVPEELVLEVNETPVAGLTIRDVLAVVKHCKEPIRIKCVKQGGIVDKDLRRYLNLRFQKGSVDHELQQIIRDNLYLRTVPCTTRQPKEGEVPGVDYNFITVEAFMELEKSGALLESGTYEENYYGTPKPPAEPAPLLLNATDHIFPGGIPRVEGKRKRNKSVSNMEKTGIEPPEEEEEERPVVNGNGVAVTPESSEHEDKSTDASGEISSLPYTAPLYCHSEEKEGTDTIKILKPDDADELGSLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENELPYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHSMPSTELGTQPMQPQVFREKPLFTRDASQLKGTFLSTILKKSNMGFGFTIIGGDEPDEFLQVKSVIPEGPAAQDGKMETGDVIVYINEACVLGYTHADVVKIFQSVPIGQSVNLVLCRGYPLPYDPEDSANNLVSPLAVIERPPVVVNGRNNYETYLEYISRTSQSVPDVAERPLPSLHSVPTDSPLENTFPPPPPTHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQILDVQGCPGLFEGDLIVEINQQNVQNLNHLEVVELLKECPVGNETSLVIQRGGFFSPWKSSKPIMERWENQGSPQTSLSAPSLPPNVPFAPMLHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRQQVPPRTGFRVDGVGPDYKELDVHLRRQESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLRPGDELLYVDGIPVAGKTHRYVIDLMHNAARNGQVNLTVRRKVLSIADTCPENGRSPGSASTQHSSPRSDSAYPNSNHSAPCNPSPPEGFTSQSMQSSDVVIHRKENEGFGFVIISSLNRPESGATIAVPHKIGRIIEGSPADRCGKLKVGDRILAVNSQSIINMPHADIVKLIKDAGLSVTLCIVPQEELNSPASAPSSEKQSPMAQQHSPMAQQSPVAQQSPVAHSLPPQPLQLQGHESSYRSEVKARQDVKPDIRQPPFTDYRQPSTDYRQPPVDYRHPPVVDYQQPPPIDYRQPPLIEYRQHSPDTRQFPLPDYRQFQDYDYFTVELEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRDMTHARAIELIKSGGRRVKLLLKRGTGHVPEYGCSSPSFRNGSFFQSLHVHEK

Target ORF information:

RefSeq Version	XM_031897516.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 2 (magi2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897516.1

ORF Insert Sequence:

ATGTCCAAGA GCCTGAAAAG GAAAAACCAC TGGACAAACA AAGTCCACGA GTGTGTGATT 
GAGAGGGGCC AGGAAGGGGA GCTGGGCTTT GAGATCAGAG GGGGAGCAGA GAGTGGGCAC 
TTCCCCTACC TTGGAGAGGT AAAGCCTGGC AAGGTGTCTT ATCAGAGTGG CAAGCTAGTG 
CCAGAGGAAC TAGTCCTAGA GGTGAATGAG ACCCCAGTGG CCGGACTTAC TATCAGGGAT 
GTACTGGCTG TCGTCAAACA CTGTAAGGAG CCCATTAGGA TCAAATGTGT GAAACAAGGA 
GGAATCGTTG ATAAAGACCT TCGCCGCTAC CTCAATTTAA GATTCCAGAA GGGTTCAGTA 
GATCATGAAC TACAACAGAT AATAAGAGAC AACCTCTACC TCCGGACAGT GCCATGTACC 
ACCAGACAGC CTAAAGAAGG GGAGGTGCCT GGAGTGGACT ACAACTTTAT CACTGTGGAA 
GCTTTTATGG AATTAGAGAA AAGTGGAGCT CTCTTGGAAA GTGGAACTTA TGAAGAAAAT 
TACTACGGAA CTCCAAAGCC TCCTGCTGAA CCTGCTCCAC TTTTATTAAA TGCAACAGAC 
CATATTTTTC CTGGTGGCAT TCCAAGAGTT GAGGGAAAAC GTAAACGCAA TAAGTCTGTA 
AGCAACATGG AGAAAACAGG GATTGAGCCT CCAGAAGAAG AGGAAGAAGA GAGGCCTGTG 
GTAAATGGAA ATGGTGTTGC AGTGACCCCA GAATCAAGTG AACATGAAGA TAAGAGTACA 
GATGCCTCAG GTGAGATCTC TTCCCTGCCT TACACTGCTC CGCTGTACTG CCATTCAGAA 
GAAAAGGAAG GCACAGACAC AATCAAGATC CTAAAGCCTG ATGACGCTGA TGAGCTGGGG 
TCTTTGCCTG ATAACTGGGA AATGGCATAT ACAGAAAAAG GAGAAGTCTA CTTTATTGAC 
CACAACACAA AGACAACGTC ATGGCTGGAT CCACGGTTAG CAAAAAAAGC AAAGCCTCCA 
GAAGAGTGCA AAGAGAATGA GTTACCTTAT GGCTGGGAGA AAATCGATGA TCCAATATAT 
GGCACGTATT ATGTTGATCA CATAAACAGA AGAACTCAGT TTGAAAACCC AGTATTAGAA 
GCAAAAAGAA AGCTTCAGCA GCACAGTATG CCCTCAACAG AACTGGGAAC CCAGCCGATG 
CAGCCCCAAG TATTTAGAGA AAAGCCACTG TTTACCCGTG ATGCATCACA ACTAAAGGGA 
ACTTTTCTAA GTACCATACT GAAGAAGAGT AATATGGGTT TTGGATTTAC CATCATTGGA 
GGAGATGAAC CAGATGAGTT TCTGCAGGTA AAGAGTGTAA TCCCTGAAGG CCCAGCTGCA 
CAGGATGGTA AAATGGAGAC AGGTGATGTA ATTGTCTATA TAAATGAAGC GTGTGTGCTT 
GGGTACACAC ATGCGGATGT GGTCAAAATT TTCCAGTCTG TTCCCATTGG GCAAAGTGTC 
AACTTGGTGC TATGTCGAGG ATATCCCCTA CCCTACGACC CAGAAGATTC TGCAAACAAC 
TTGGTATCAC CTTTAGCAGT CATTGAAAGG CCGCCAGTGG TTGTAAATGG AAGAAATAAC 
TATGAAACTT ATTTGGAATA CATTTCTAGG ACGTCACAAT CAGTACCAGA TGTAGCAGAA 
CGACCATTGC CTTCTTTGCA CTCTGTTCCA ACAGACAGTC CGCTTGAAAA TACATTTCCA 
CCGCCTCCAC CTACACATGA TGATAATGTA TCAATGGCTT CTTCTGGAGC CACACAAGCA 
GAACTTATGA CCTTAACCAT TGTGAAAGGG GCACAGGGCT TTGGCTTCAC AATAGCAGAC 
AGTCCAACAG GACAGCGAGT GAAGCAGATT TTAGATGTTC AGGGATGCCC TGGTTTGTTT 
GAAGGGGACC TTATAGTTGA GATCAACCAG CAGAATGTAC AGAATTTGAA CCACTTAGAA 
GTTGTGGAGT TACTTAAAGA ATGTCCTGTT GGAAATGAGA CTTCTTTGGT GATCCAGAGA 
GGAGGTTTCT TTTCTCCGTG GAAAAGTTCC AAGCCTATAA TGGAAAGATG GGAAAACCAA 
GGAAGCCCTC AGACTAGTTT ATCAGCACCT TCCTTACCAC CAAATGTCCC TTTTGCTCCA 
ATGCTACATA GGAGTTCATT TCCCGATTCC ACAGAGGCCT TTGACCCTAG AAAACCTGAC 
CCCTATGAAC TATATGAAAA ATCAAGAGCA ATTTATGAAA GTAGGCAGCA AGTGCCGCCC 
AGGACTGGTT TCAGAGTGGA TGGAGTTGGC CCTGACTATA AGGAGTTGGA TGTTCATCTT 
CGGCGCCAAG AATCTGGATT TGGGTTCAGA ATCCTTGGAG GAGATGAACC TGGCCAGCCT 
ATTTTAATTG GAGCTGTAAT AGCGATGGGC TCTGCAGACA GAGATGGACG CCTACGCCCT 
GGCGATGAGT TGTTATACGT AGATGGAATC CCAGTTGCTG GAAAAACCCA CCGATATGTG 
ATTGATCTTA TGCATAATGC TGCTCGGAAT GGACAGGTCA ACCTTACTGT GAGACGAAAG 
GTGCTATCTA TAGCAGATAC ATGTCCAGAA AATGGCAGGA GTCCTGGCTC TGCCTCAACA 
CAACACAGTT CTCCCCGAAG TGATTCTGCT TATCCAAACA GCAACCATTC TGCACCATGC 
AATCCTTCTC CACCAGAGGG TTTCACGTCA CAAAGCATGC AGTCCAGTGA TGTGGTTATA 
CATCGAAAGG AGAATGAAGG ATTTGGTTTT GTCATAATAA GTTCTTTGAA CAGACCAGAA 
TCAGGCGCAA CAATTGCTGT GCCACATAAA ATCGGACGAA TTATTGAAGG AAGCCCTGCA 
GATCGCTGTG GAAAGCTAAA AGTCGGAGAC CGGATCTTAG CTGTGAATAG CCAATCTATT 
ATCAACATGC CTCATGCAGA TATTGTAAAG CTGATTAAGG ATGCAGGGCT GAGTGTAACA 
CTTTGTATCG TTCCTCAGGA GGAATTAAAC AGTCCAGCTT CAGCACCAAG CTCAGAAAAA 
CAGAGTCCTA TGGCACAGCA GCACAGCCCA ATGGCACAGC AGAGCCCCGT AGCTCAGCAA 
AGTCCTGTGG CACACTCACT GCCCCCTCAA CCACTACAGT TGCAAGGCCA TGAGAGCAGC 
TATAGGTCAG AAGTAAAAGC AAGGCAAGAT GTAAAACCAG ACATCAGGCA ACCACCATTT 
ACAGATTATC GCCAGCCATC AACGGACTAT AGGCAGCCTC CTGTAGATTA CAGACATCCT 
CCAGTAGTGG ATTACCAGCA GCCTCCTCCT ATAGACTACA GACAGCCACC TTTAATAGAG 
TACAGGCAAC ATTCTCCCGA TACTCGGCAA TTTCCTCTCC CTGATTACCG ACAATTTCAG 
GATTATGATT ATTTTACTGT TGAACTGGAA AAGGGTGCTA AGGGTTTTGG ATTTAGTATA 
CGAGGAGGAA GAGAATACAA GATGGATTTG TATGTGTTAA GGCTGGCAGA AGATGGCCCT 
GCAATAAGAA ATGGACGAAT GAGGGTTGGT GATCAAATCA TAGAAATCAA TGGGGAAAGC 
ACAAGAGACA TGACACATGC CAGAGCAATA GAACTCATTA AATCAGGAGG AAGAAGAGTA 
AAGCTTTTAC TGAAGCGTGG AACAGGTCAT GTTCCTGAGT ATGGCTGTTC CTCCCCTTCC 
TTTAGGAATG GTTCCTTCTT CCAGTCTCTC CATGTGCATG AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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magi2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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