slc12a2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following slc12a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03458 XM_004910473.4
Latest version!
Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03458 XM_004910472.4
Latest version!
Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03458 XM_004910470.4
Latest version!
Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03458 XM_004910471.4
Latest version!
Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03458 XM_002931709.5
Latest version!
Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa03458 XM_004910474.4
Latest version!
Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa30158 XM_012968272.2
Latest version!
Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OXa03458 XM_004910471.3 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa03458 XM_004910472.3 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa03458 XM_002931709.4 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa03458 XM_004910473.3 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa03458 XM_004910470.3 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa03458 XM_004910474.3 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910471.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910471.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910528.1
    CDS341..3826
    Translation

    Target ORF information:

    RefSeq Version XM_004910471.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910471.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910473.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910473.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910530.1
    CDS142..3627
    Translation

    Target ORF information:

    RefSeq Version XM_004910473.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910473.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910472.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910472.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910529.1
    CDS262..3747
    Translation

    Target ORF information:

    RefSeq Version XM_004910472.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910472.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_002931709.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931709.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931755.1
    CDS180..3665
    Translation

    Target ORF information:

    RefSeq Version XM_002931709.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931709.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910470.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910470.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910527.1
    CDS370..3855
    Translation

    Target ORF information:

    RefSeq Version XM_004910470.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910470.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910474.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910474.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910531.1
    CDS160..3645
    Translation

    Target ORF information:

    RefSeq Version XM_004910474.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 2 (slc12a2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910474.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910472.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910529.1
    CDS271..3756
    Translation

    Target ORF information:

    RefSeq Version XM_004910472.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910472.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910474.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910531.1
    CDS95..3580
    Translation

    Target ORF information:

    RefSeq Version XM_004910474.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910474.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910470.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910470.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910527.1
    CDS379..3864
    Translation

    Target ORF information:

    RefSeq Version XM_004910470.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910470.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910471.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910471.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910528.1
    CDS350..3835
    Translation

    Target ORF information:

    RefSeq Version XM_004910471.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910471.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_002931709.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931709.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931755.1
    CDS147..3632
    Translation

    Target ORF information:

    RefSeq Version XM_002931709.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931709.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03458
    Clone ID Related Accession (Same CDS sequence) XM_004910472.3 , XM_004910474.3 , XM_004910471.4 , XM_004910473.4 , XM_004910470.3 , XM_004910471.3 , XM_004910472.4 , XM_002931709.5 , XM_002931709.4 , XM_004910473.3 , XM_004910470.4 , XM_004910474.4
    Accession Version XM_004910473.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910530.1
    CDS156..3641
    Translation

    Target ORF information:

    RefSeq Version XM_004910473.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910473.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAACACACCG CCAGATTCGA
    TTGAATGAGT TGTTGAAAGA GCACTCAAGC ACAGCAAATG CCATTGTTAT GAGCCTTCCA
    GTTGCAAGAA AAAGTGCCGT ATCTAGTGCC CTTTATATGG CTTGGATAGA AGCTCTTTCT
    AAAGACCTGC CACCAATCCT TCTTGTTCGT GGGAATCATC AGAGCGTGCT TACATTCTAC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30158
    Clone ID Related Accession (Same CDS sequence) XM_012968272.2
    Accession Version XM_012968272.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAGAGCCTTC CAGTTGCAAG
    AAAAAGTGCC GTATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823726.1
    CDS380..3697
    Translation

    Target ORF information:

    RefSeq Version XM_012968272.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968272.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGCAAG CAGGAAAACC CGGCTCAGAG ATACCTGAGC AGGGAAAGTC CCCATCCAGT 
    TCTCTTAACC CTCAACCTTC ACAGTCTGCT TTTCCATCAA GCCAGGAATT GCAAGTTGAT
    GATGCACTGG CTGGGCGACC CGTGGGGCCT ACTCCAAGCC AAAGCCGTTT TCAAGTGGAT
    CTGGTTACTG AGGGATCTAA GGAGGCAGCT GCACAGGAAC TGCAACAGCA AGCAGATGGC
    ATCACACAAA GGGGTAGAGG TGGCTCAGGC AGTGAGGAGG CAAAAGGGCG CTTTAGGGTT
    AATTTTGTAG ATCCGGCTTC TCCTGGAGGT AGTTCACCAG AAAATGCAGG GGTTGATGGT
    GGTGGTGGTG GAGTGAGCTT CCAAAATGGA GACACTGTCC TAAGTGAAGG GAGTTTACAT
    TCTGGTGGAA CTGGACATCA CTATCATTAC GACACGCATA CTAATACTTA CTACCTTCGC
    ACTTTTGGGC ACAACACAAT TGATGCAGTG CCAAGGATAG ACCATTACCG GCACACAGCT
    GCTGACCTTG GCGAAAAGCT ACTGAGACCC AGCTTGGCAG AGCTTCATGA TGAACTGGAC
    AAGGAACCCT TTGAAGATGG CTTTGCAAAT GGTGAAGAAG GAACACCAAC AGGCGATGCA
    GCTACTACTG TTGCAGATAG TAAAGGCGTA GTGAAATTTG GTTGGATTAA AGGTGTTTTG
    GTCCGATGTA TGCTAAATAT ATGGGGTGTG ATGCTCTTCA TCAGGTTGTC CTGGATTGTG
    GGCCAGGCTG GCATTGGGCT AACTGTTGTA GTCATATCAA TGGCTACTGT GGTGACCACC
    ATCACAGGAC TGTCTACATC AGCTATTGCC ACCAATGGAT TTGTGAGAGG AGGGGGTGCA
    TACTACCTGA TATCTCGCAG TCTTGGTCCA GAATTTGGAG GAGCAATTGG ACTGATTTTT
    GCATTTGCAA ATGCTGTAGC AGTAGCAATG TATGTGGTGG GTTTTGCAGA GACTGTTGTC
    GAACTACTTA AGGAAAACGG TGCTCTTATG GTGGATGACA TTAATGATAT ACGAATTATT
    GGTGCAATCA CAGTTGTGCT GTTGCTTGGA ATCTCTGTTG CAGGAATGGA GTGGGAAGCA
    AAGGCACAAA TTGTTCTTCT AGTCATTCTG CTCTTGGCAA TTGTAGACTT CTTTATTGGA
    ACAGTCATTC CATCTGAAGA CAAGAAACCA AAGGGATTTT TTGGTTATAA AGCCGATATT
    TTCAGTGAAA ACTTTGGACC AGATTTCCGA GAAGGTGAAA CTTTTTTCTC GGTTTTTGCA
    ATTTTCTTTC CTGCTGCAAC TGGCATCTTA GCAGGAGCAA ATATCTCAGG TGATCTTGCG
    GACCCTCAAC ATGCCATACC TAGAGGAACA CTACTGGCCA TTCTTATTAC AACTGTGGTC
    TACATGGGCG TTGCAGTCTC TGTGGGGTCA TGTGTTGTCC GTGATGCTTC TGGGGATGTT
    AATAGCACCC TATCCGCAGC CATGACAAAT TGCACTACTG CAGCATGCAA TCTGAATTAT
    GACTTTTCAT CTTGTGGGCA TGAGTGTAAA TATGGATTAA TGAACAGCTT TCAGGTAATG
    AGCATGGTCT CTGGATTTGC ACCCCTCATC ACTGCAGGAA TCTTCTCTGC TACTCTGTCA
    TCTGCCCTTG CCTCTTTAGT GAGTGCACCA AAGGTCTTCC AGGCTTTGTG TAAAGACAAC
    ATATATCCTG GGCTCCAGAT GTTTGCTAAA GGATATGGAA AGAACAATGA ACCCCTCCGT
    GGCTACCTTC TTACTTTCAT TATCGCCCTG GGATTTATAT TGATTGCCGA GCTAAACCTG
    ATTGCACCCA TCATCTCCAA CTTCTTTCTG GCATCATACG CCTTAATAAA CTTTTCTGTG
    TTCCACGCCT CCCTTGCCAA GTCTCCAGGA TGGCGCCCTG CCTTCAGATA CTACAACATG
    TGGGTGTCAC TCATTGGAGC TCTCCTGTGC TGTGGAGTCA TGTTTGTCAT AAACTGGTGG
    GCTGCTCTTT TGACATATGT TATCGTAATT GGATTGTATA TATACGTCAC ATATAAGAAG
    CCTGATGTCA ACTGGGGATC CTCCACACAA GCCTTGACAT ACCTAAATGC TTTGCAACAC
    ACTATTCGTC TTGCTGGTGT AGAAGACCAT GTGAAAAATT TCAGGCCGCA GTGTCTTGTT
    TTAATAGGAG CACCAAATTC ACGTCCAGCT TTGCTTCACT TAGTACATGC CTTCACCAAA
    AATGTTGGCT TGATGATCTG TGGACATGTA CATATGGGTC CTCGCAGACA AGCGATGAAA
    GAACTACTTA CAGATCAAGC ACGATACCAG AGGTGGCTCA TCAAAAACAA GACTAAGGCA
    TTCTATTCAC CTGTCCATGC AGAAGACTTA CGGGATGGAG CACAGTATCT TATGCAGGCT
    GCTGGATTAG GACGGATGAG GCCAAATACA CTTGTGGTTG GCTTTAAGAA GAACTGGAGT
    CAATGTGATA TGAGAGAAGT GGAAACCTAC ATTAACTTGT TTCATGATGC CTTCGACTTT
    CAGTATGGTG TGGTTGTTAT CCGATTGAAG GAAGGCTTAG ATATTTCTCA TCTCCAAGGG
    CAAGAGGAGT TGTTGTCATC TCAAGAGAAA TCTCCTGGCA CCAAAGATGT TGTGGTTAAT
    GTAGACTACA GCAAAAAGGC TGAAGCAGAA TCTTCAAGAC CATTTGCCAG TGACAGCAGT
    CCTATGATGC GGAAAGATGA GGAAGACGAT GGCAAGACAC CAACACAACC ACTGTTGAAA
    AAAGACTCAA AAGGCCAAGG CATTTCACTG GACACTGCTG ACCAAAGGCT TTTGGATGCT
    AGCTTACAGT TTCAGAAGAA GCAAGGCAAA AGCACCATTG ATGTTTGGTG GCTTTTTGAT
    GATGGAGGTT TAACATTGTT AATTCCATAT TTGATTACTT CAAAGAAGAA GTGGAGAGAC
    TGTAAGATTC GAGTATTTAT TGGTGGCAAA ATCAATAGGA TAGATCATGA CAGAAGAGCC
    ATGGCAACCT TACTCAGCAA GTTCAGAATT GACTTCTCTG ACATCATGGT CCTTGGGGAT
    ATCAACACAA AACCAAAGAA AGAGAATGTT GCAGCTTTTG AAGAGATGAT TGAACCATTT
    AGACTTCACG AGGATGAAAA AGATCAAGAG GTTGCAGAGA AAATGAAAGA AGAAGAGCCC
    TGGAGAATTA CAGATAATGA GTTAGAGCTT TATAAAACAA AAGAGCCTTC CAGTTGCAAG
    AAAAAGTGCC GTATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.