pole4.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pole4.L
Entrez Gene ID	779084
Full Name	polymerase (DNA-directed), epsilon 4, accessory subunit L homeolog
Synonyms	pole4
General protein information	Preferred Names polymerase (DNA-directed), epsilon 4, accessory subunit L homeolog Names DNA-directed DNA polymerase epsilon 4 DNA polymerase epsilon p12 subunit polymerase (DNA-directed), epsilon 4 (p12 subunit)
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001096724.1	NP_001090193.1	DNA-directed DNA polymerase epsilon 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts.
Shikata K, Sasa-Masuda T, Okuno Y, Waga S, Sugino A
BMC biochemistry721(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNA polymerase activity of Pol epsilon holoenzyme is essential for chromosomal DNA replication in higher eukaryotes.
Title: The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts.

The following pole4.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pole4.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf07200	NM_001096724.1 Latest version!	Xenopus laevis polymerase (DNA-directed), epsilon 4, accessory subunit L homeolog (pole4.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pole4.L ( NM_001096724.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001090193.1 Xenopus laevis polymerase (DNA-directed), epsilon 4, accessory subunit L homeolog (pole4.L), mRNA.

Price & Availability↑

CloneID	OAf07200
Clone ID Related Accession (Same CDS sequence)	NM_001096724.1
Accession Version	NM_001096724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-01
Organism	Xenopus laevis(African clawed frog)
Product	DNA-directed DNA polymerase epsilon 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB259048.1. ##Evidence-Data-START## Transcript exon combination :: AB259048.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG AGGTAGCAGC ACCGGAGTCG CTGGAGGGAA GTGGCAGTCA GGAAGACGCG 
GGTCCTAGCA GTCCCCCTGC AGCACACCCA GCTCCGTGTC CTTCGAAACA AGCCAGGCTG 
CCGCTGTCCC GGATAAAAGC TTTGATGAAA GCGGATCCGG ACCTTTCATT GGCCAGTCAG 
GAGTCCGTGT TTGTCATTAG TAAAGCGACG GAATTATTTA TAGAAACCAT TGCCAAGGAT 
GCTTACCTAT ATGCGCAGCA GGGAAAGCGA AAAACACTGC AAAGGAAAGA TTTGGATAAT 
GCTATAGATG CCATTGACGA GTTTGCTTTC CTGGAGGGTA CTTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001090193.1
CDS	10..360
Translation	MAAEVAAPESLEGSGSQEDAGPSSPPAAHPAPCPSKQARLPLSRIKALMKADPDLSLASQESVFVISKATELFIETIAKDAYLYAQQGKRKTLQRKDLDNAIDAIDEFAFLEGTLD

Target ORF information:

RefSeq Version	NM_001096724.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis polymerase (DNA-directed), epsilon 4, accessory subunit L homeolog (pole4.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001096724.1

ORF Insert Sequence:

ATGGCGGCAG AGGTAGCAGC ACCGGAGTCG CTGGAGGGAA GTGGCAGTCA GGAAGACGCG 
GGTCCTAGCA GTCCCCCTGC AGCACACCCA GCTCCGTGTC CTTCGAAACA AGCCAGGCTG 
CCGCTGTCCC GGATAAAAGC TTTGATGAAA GCGGATCCGG ACCTTTCATT GGCCAGTCAG 
GAGTCCGTGT TTGTCATTAG TAAAGCGACG GAATTATTTA TAGAAACCAT TGCCAAGGAT 
GCTTACCTAT ATGCGCAGCA GGGAAAGCGA AAAACACTGC AAAGGAAAGA TTTGGATAAT 
GCTATAGATG CCATTGACGA GTTTGCTTTC CTGGAGGGTA CTTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The DNA polymerase activity of Pol epsilon holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts.
BMC biochemistry721(2006 Aug)
Shikata K,Sasa-Masuda T,Okuno Y,Waga S,Sugino A