ccnb1 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccnb1
Entrez Gene ID	379888
Full Name	cyclin B1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018256474.1	XP_018111963.1	cyclin B1 isoform X1
NM_001086727.1	NP_001080196.1	cyclin B1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Analyses of zebrafish and Xenopus oocyte maturation reveal conserved and diverged features of translational regulation of maternal cyclin B1 mRNA.
Zhang Y, Sheets MD
BMC developmental biology97(2009 Jan)

Cyclin B dissociation from CDK1 precedes its degradation upon MPF inactivation in mitotic extracts of Xenopus laevis embryos.
Chesnel F, Bazile F, Pascal A, Kubiak JZ
Cell cycle (Georgetown, Tex.)5(15)1687-98(2006 Aug)

The cytoskeleton-dependent localization of cdc2/cyclin B in blastomere cortex during Xenopus embryonic cell cycle.
Nakamura N, Tokumoto T, Ueno S, Iwao Y
Molecular reproduction and development72(3)336-45(2005 Nov)

Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx.
Walsh S, Margolis SS, Kornbluth S
Molecular cancer research : MCR1(4)280-9(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

zebrafish cyclin B1 3'UTR was quantitatively less effective at stimulating polyadenylation and translation compared to the Xenopus cyclin B1 3'UTR during both zebrafish and Xenopus oocyte maturation
Title: Analyses of zebrafish and Xenopus oocyte maturation reveal conserved and diverged features of translational regulation of maternal cyclin B1 mRNA.

Cyclin B dissociation from CDK1 precedes cyclins B degradation upon CDK1 inactivation in mitotic embryo extracts and that proteasome proteolytic activity is dispensable for both activation and inactivation of CDK1 in such extracts.
Title: Cyclin B dissociation from CDK1 precedes its degradation upon MPF inactivation in mitotic extracts of Xenopus laevis embryos.

Cyclin B1 displays cytoskeleton-dependent localization in blastomere cortex during Xenopus embryonic cell cycle.
Title: The cytoskeleton-dependent localization of cdc2/cyclin B in blastomere cortex during Xenopus embryonic cell cycle.

new role for Erk in mitotic regulation, identification of the Ser 101-directed kinase, and a picture of cyclin B1/Cdc2 regulation by the combinatorial action of distinct kinases
Title: Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx.

The following ccnb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccnb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf08115	NM_001086727.1 Latest version!	Xenopus laevis cyclin B1 (ccnb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OAf27553	XM_018256474.1 Latest version!	Xenopus laevis cyclin B1 (ccnb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccnb1 ( NM_001086727.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001080196.1 Xenopus laevis cyclin B1 (ccnb1), mRNA.

Price & Availability↑

CloneID	OAf08115
Clone ID Related Accession (Same CDS sequence)	NM_001086727.1
Accession Version	NM_001086727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Xenopus laevis(African clawed frog)
Product	cyclin B1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC041302.1. ##Evidence-Data-START## Transcript exon combination :: BC041302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCTGC GTATCACCAG AAACATGATG GCAAATACAG AAAACAATGT GAAAACCACT 
TTGGCTGGAA AGAGGGTTGT TGCCACCAAA CCAGGCTTGA GACCTCGTAC AGCATTGGGA 
GACATTGGAA ACAAGGCAGA GCTGAAAGTG CCAGCAAAAA AGGAATTAAA GCCAGCAGTA 
AAGGCTGTCA AGAAGACAAA ACCCATTGAC AAAGTTTTGG AGCCTCTGAA AGTCAGTGAA 
GAGAATGTTT GCCCTAAACC TACTCCGGTT GAACCCAGCT CACCAAGCCC AATGGAAACA 
TCTGGTTGCC TCCCTGATGA GCTTTGCCAG GCCTTCTCTG ATGTCCTGAT TCAAGTTAAA 
GATGTTGATG CTGATGATGA TGGCAACCCA ATGCTGTGCA GTGAATATGT CAAAGACATT 
TATGGCTACC TGAGAAGTCT TGAGGATGCA CAAGCAGTCA GACCAAATTA CCTACATGGA 
CGGGAAGTTA CAGGCAACAT GCGTGCTATT TTGATCGACT GGCTGGTCCA GGTGCAAATG 
AAGTTCCGTC TGCTGCAGGA GACCATGTTT ATGACTGTTG GCATTATTGA CCGTTTTCTG 
CAGGAACACC CAGTTCCCAA AAACCAGCTG CAGCTTGTGG GGGTCACCGC AATGTTCCTT 
GCTGCTAAAT ACGAAGAGAT GTACCCACCA GAAATTGGGG ACTTTACTTT TGTAACTGAT 
CACACATACA CAAAGGCTCA AATTAGAGAC ATGGAAATGA AGATACTTCG TGTGCTAAAG 
TTTGCAATTG GCCGACCTCT ACCCCTGCAC TTCCTTCGGA GAGCATCTAA GATTGGAGAG 
GTGACTGCTG AGCAGCATAG TTTGGCCAAA TATTTGATGG AACTTGTGAT GGTGGATTAT 
GACATGGTGC ATTATGCTCC TTCCCAAATA GCAGCTTCTG CCTCCCGCTT GTCTCTTAAA 
ATCTTAAATG CAGGTGACTG GACCCCAACA CTGCATCACT ATATGGCTTA CTCTGAAGAA 
GATCTGGTCC CTGTTATGCA GCATATGGCC AAGAACATAA TCAAGGTGAA CAAAGGACTG 
ACTAAGCACC TGACTGTTAA GAACAAATAT GCCAGCAGCA AACAAATGAA GATCAGCATG 
ATTCCACAAT TGAAATCGGA TGTTGTTGTG GCAATGGCCC GCCCACTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001080196.1
CDS	47..1240
Translation	MSLRITRNMMANTENNVKTTLAGKRVVATKPGLRPRTALGDIGNKAELKVPAKKELKPAVKAVKKTKPIDKVLEPLKVSEENVCPKPTPVEPSSPSPMETSGCLPDELCQAFSDVLIQVKDVDADDDGNPMLCSEYVKDIYGYLRSLEDAQAVRPNYLHGREVTGNMRAILIDWLVQVQMKFRLLQETMFMTVGIIDRFLQEHPVPKNQLQLVGVTAMFLAAKYEEMYPPEIGDFTFVTDHTYTKAQIRDMEMKILRVLKFAIGRPLPLHFLRRASKIGEVTAEQHSLAKYLMELVMVDYDMVHYAPSQIAASASRLSLKILNAGDWTPTLHHYMAYSEEDLVPVMQHMAKNIIKVNKGLTKHLTVKNKYASSKQMKISMIPQLKSDVVVAMARPLV

Target ORF information:

RefSeq Version	NM_001086727.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cyclin B1 (ccnb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001086727.1

ORF Insert Sequence:

ATGTCGCTGC GTATCACCAG AAACATGATG GCAAATACAG AAAACAATGT GAAAACCACT 
TTGGCTGGAA AGAGGGTTGT TGCCACCAAA CCAGGCTTGA GACCTCGTAC AGCATTGGGA 
GACATTGGAA ACAAGGCAGA GCTGAAAGTG CCAGCAAAAA AGGAATTAAA GCCAGCAGTA 
AAGGCTGTCA AGAAGACAAA ACCCATTGAC AAAGTTTTGG AGCCTCTGAA AGTCAGTGAA 
GAGAATGTTT GCCCTAAACC TACTCCGGTT GAACCCAGCT CACCAAGCCC AATGGAAACA 
TCTGGTTGCC TCCCTGATGA GCTTTGCCAG GCCTTCTCTG ATGTCCTGAT TCAAGTTAAA 
GATGTTGATG CTGATGATGA TGGCAACCCA ATGCTGTGCA GTGAATATGT CAAAGACATT 
TATGGCTACC TGAGAAGTCT TGAGGATGCA CAAGCAGTCA GACCAAATTA CCTACATGGA 
CGGGAAGTTA CAGGCAACAT GCGTGCTATT TTGATCGACT GGCTGGTCCA GGTGCAAATG 
AAGTTCCGTC TGCTGCAGGA GACCATGTTT ATGACTGTTG GCATTATTGA CCGTTTTCTG 
CAGGAACACC CAGTTCCCAA AAACCAGCTG CAGCTTGTGG GGGTCACCGC AATGTTCCTT 
GCTGCTAAAT ACGAAGAGAT GTACCCACCA GAAATTGGGG ACTTTACTTT TGTAACTGAT 
CACACATACA CAAAGGCTCA AATTAGAGAC ATGGAAATGA AGATACTTCG TGTGCTAAAG 
TTTGCAATTG GCCGACCTCT ACCCCTGCAC TTCCTTCGGA GAGCATCTAA GATTGGAGAG 
GTGACTGCTG AGCAGCATAG TTTGGCCAAA TATTTGATGG AACTTGTGAT GGTGGATTAT 
GACATGGTGC ATTATGCTCC TTCCCAAATA GCAGCTTCTG CCTCCCGCTT GTCTCTTAAA 
ATCTTAAATG CAGGTGACTG GACCCCAACA CTGCATCACT ATATGGCTTA CTCTGAAGAA 
GATCTGGTCC CTGTTATGCA GCATATGGCC AAGAACATAA TCAAGGTGAA CAAAGGACTG 
ACTAAGCACC TGACTGTTAA GAACAAATAT GCCAGCAGCA AACAAATGAA GATCAGCATG 
ATTCCACAAT TGAAATCGGA TGTTGTTGTG GCAATGGCCC GCCCACTTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccnb1 ( XM_018256474.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018111963.1 Xenopus laevis cyclin B1 (ccnb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf27553
Clone ID Related Accession (Same CDS sequence)	XM_018256474.1
Accession Version	XM_018256474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	cyclin B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC GTATCACCAG AAACATGATG GCAAATACAG AAAACAATGT GAAAACCACT 
TTGGCTGGAA AGAGGGTTGT TGCCACCAAA CCAGGCTTGA GACCTCGTAC AGCATTGGGA 
GACATTGGAA ACAAGGCAGA GCTGAAAGTG CCAGCAAAAA AGGAATTAAA GCCAGCAGTA 
AAGGCTGTCA AGAAGACAAA ACCCATTGAC AAAGTTTTGG AGCCTCTGAA AGTCAGTGAA 
GAGAATGTTT GCCCTAAACC TACTCCGGTT GAACCCAGCT CACCAAGCCC AATGGAAACA 
TCTGGTTGCC TCCCTGATGA GCTTTGCCAG GCCTTCTCTG ATGTCCTGAT TCAAGTTAAA 
GATGTTGATG CTGATGATGA TGGCAACCCA ATGCTGTGCA GTGAATATGT CAAAGACATT 
TATGGCTACC TGAGAAGTCT TGAGGATGCA CAAGCAGTCA GACCAAATTA CCTACATGGA 
CGGGAAGTTA CAGGCAACAT GCGTGCTATT TTGATCGACT GGCTGGTCCA GGTGCAAATG 
AAGTTCCGTC TGCTGCAGGA GACCATGTTT ATGACTGTTG GCATTATTGA CCGTTTTCTG 
CAGGAACACC CAGTTCCCAA AAACCAGCTG CAGCTTGTGG GGGTCACCGC AATGTTCCTT 
GCTGCTAAAT ACGAAGAGAT GTACCCACCA GAAATTGGGG ACTTTACTTT TGTAACTGAT 
CACACATACA CAAAGGCTCA AATTAGAGAC ATGGAAATGA AGATACTTCG TGTGCTAAAG 
TTTGCAATTG GCCGACCTCT ACCCCTGCAC TTCCTTCGGA GAGCATCTAA GATTGGAGAG 
ACCCCAACAC TGCATCACTA TATGGCTTAC TCTGAAGAAG ATCTGGTCCC TGTTATGCAG 
CATATGGCCA AGAACATAAT CAAGGTGAAC AAAGGACTGA CTAAGCACCT GACTGTTAAG 
AACAAATATG CCAGCAGCAA ACAAATGAAG ATCAGCATGA TTCCACAATT GAAATCGGAT 
GTTGTTGTGG CAATGGCCCG CCCACTTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018111963.1
CDS	88..1140
Translation	MSLRITRNMMANTENNVKTTLAGKRVVATKPGLRPRTALGDIGNKAELKVPAKKELKPAVKAVKKTKPIDKVLEPLKVSEENVCPKPTPVEPSSPSPMETSGCLPDELCQAFSDVLIQVKDVDADDDGNPMLCSEYVKDIYGYLRSLEDAQAVRPNYLHGREVTGNMRAILIDWLVQVQMKFRLLQETMFMTVGIIDRFLQEHPVPKNQLQLVGVTAMFLAAKYEEMYPPEIGDFTFVTDHTYTKAQIRDMEMKILRVLKFAIGRPLPLHFLRRASKIGETPTLHHYMAYSEEDLVPVMQHMAKNIIKVNKGLTKHLTVKNKYASSKQMKISMIPQLKSDVVVAMARPLV

Target ORF information:

RefSeq Version	XM_018256474.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cyclin B1 (ccnb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018256474.1

ORF Insert Sequence:

ATGTCGCTGC GTATCACCAG AAACATGATG GCAAATACAG AAAACAATGT GAAAACCACT 
TTGGCTGGAA AGAGGGTTGT TGCCACCAAA CCAGGCTTGA GACCTCGTAC AGCATTGGGA 
GACATTGGAA ACAAGGCAGA GCTGAAAGTG CCAGCAAAAA AGGAATTAAA GCCAGCAGTA 
AAGGCTGTCA AGAAGACAAA ACCCATTGAC AAAGTTTTGG AGCCTCTGAA AGTCAGTGAA 
GAGAATGTTT GCCCTAAACC TACTCCGGTT GAACCCAGCT CACCAAGCCC AATGGAAACA 
TCTGGTTGCC TCCCTGATGA GCTTTGCCAG GCCTTCTCTG ATGTCCTGAT TCAAGTTAAA 
GATGTTGATG CTGATGATGA TGGCAACCCA ATGCTGTGCA GTGAATATGT CAAAGACATT 
TATGGCTACC TGAGAAGTCT TGAGGATGCA CAAGCAGTCA GACCAAATTA CCTACATGGA 
CGGGAAGTTA CAGGCAACAT GCGTGCTATT TTGATCGACT GGCTGGTCCA GGTGCAAATG 
AAGTTCCGTC TGCTGCAGGA GACCATGTTT ATGACTGTTG GCATTATTGA CCGTTTTCTG 
CAGGAACACC CAGTTCCCAA AAACCAGCTG CAGCTTGTGG GGGTCACCGC AATGTTCCTT 
GCTGCTAAAT ACGAAGAGAT GTACCCACCA GAAATTGGGG ACTTTACTTT TGTAACTGAT 
CACACATACA CAAAGGCTCA AATTAGAGAC ATGGAAATGA AGATACTTCG TGTGCTAAAG 
TTTGCAATTG GCCGACCTCT ACCCCTGCAC TTCCTTCGGA GAGCATCTAA GATTGGAGAG 
ACCCCAACAC TGCATCACTA TATGGCTTAC TCTGAAGAAG ATCTGGTCCC TGTTATGCAG 
CATATGGCCA AGAACATAAT CAAGGTGAAC AAAGGACTGA CTAAGCACCT GACTGTTAAG 
AACAAATATG CCAGCAGCAA ACAAATGAAG ATCAGCATGA TTCCACAATT GAAATCGGAT 
GTTGTTGTGG CAATGGCCCG CCCACTTGTA TGA