HMBS cDNA ORF clone, Tyto alba(Barn owl)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMBS
Entrez Gene ID	104367683
Full Name	hydroxymethylbilane synthase
Gene Type	protein-coding
Organism	Tyto alba(Barn owl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009974849.1	XP_009973151.1	porphobilinogen deaminase

Pathways from BioSystems
Gene Ontology

The following HMBS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMBS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTy07119	XM_009974849.1 Latest version!	Tyto alba hydroxymethylbilane synthase (HMBS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMBS ( XM_009974849.1 ) cDNA ORF clone, Tyto alba(Barn owl) -> XP_009973151.1 Tyto alba hydroxymethylbilane synthase (HMBS), mRNA.

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CloneID	OTy07119
Clone ID Related Accession (Same CDS sequence)	XM_009974849.1
Accession Version	XM_009974849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Tyto alba(Barn owl)
Product	porphobilinogen deaminase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010024509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tyto alba Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT CTTTGTTTAG GGCTTTGCTT TCTGCCTCGT TGCAGCTGGC CCGGATTCAG 
ACCGACAGCG TGGTCGAGAT GCTCCGTGAG CTATACCCCT ATCTGCGCTT TGAGATTGTT 
GCCATGTCAA CGACTGGAGA CAAGATCCTG GATACAGCGC TTTCCAAGAT TGGGGAGAAG 
AGCCTCTTCA CCAAAGAGCT GGAAAATGCC CTTGAAAGAA ATGAAGTTGA CCTTGTGGTT 
CACTCCTTGA AGGACCTGCC AACTTCTCTT CCTCCTGGCT TTACCATCGG TGCTGTCTGC 
AAAAGGGAAA ACCCGCTCGA TGCTGTCGTC TTTCATCCCA AAAACTGTGG GAAAACACTG 
AGCCTCCTTC CTGAAAAGAG TGTGATTGGG ACCAGTTCAC TTCGGAGAGC AGCCCAGCTC 
AAAAAGAAGT TCCCTCAGTT AGAATTCAGG GATATTAGAG GGAATTTAAA TACCCGCTTA 
AAGAAGCTGG ATGAGAAGGA AGACTTCAGT GCCATCGTTC TGGCTGCCGC TGGGCTGAAG 
AGGATGGGCT GGGAGGATCG CATTGGCCAG CTCCTAAGCC CTGAAGATTG TCTGTATGCT 
GTTGGACAGG GTGCCTTAGC AGTGGAAGTT CGTGCCAAAG ACCAAGAGAT ACTGAATATG 
GTATCTGCTC TGCACGACGC AGACACTGTG TTACGCTGCA TCGCCGAGAG AGCCTTTATG 
AAACGTCTGG AGGGTGGATG TAGCGTCCCT GTTGCAGTCA ACACTCTGCT GAAAGACGGC 
CAGTTGTATT TGACGGGAGC GGTCTACAGT TTGGACGGAT CCGATAGCCT GAAGGAGACT 
ATGCAGACCA GTGTTAATTA CCCCCAACAG AATGAAGATG GACCAAACGA CGATGTGCAG 
CACGTTGGCA TCACGGCCAA GAATGTCCCC GGTCAGGCCC AAGAAGCTGC AGAAGACCTT 
GGTGTTGAGC TAGCTAGTTT ACTTCTGAGT AAGGGAGCTA AGCATATCCT TAGCGTGGCA 
AGGCAGCTCA ACGATGCCCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009973151.1
CDS	1..1044
Translation	MMCSLFRALLSASLQLARIQTDSVVEMLRELYPYLRFEIVAMSTTGDKILDTALSKIGEKSLFTKELENALERNEVDLVVHSLKDLPTSLPPGFTIGAVCKRENPLDAVVFHPKNCGKTLSLLPEKSVIGTSSLRRAAQLKKKFPQLEFRDIRGNLNTRLKKLDEKEDFSAIVLAAAGLKRMGWEDRIGQLLSPEDCLYAVGQGALAVEVRAKDQEILNMVSALHDADTVLRCIAERAFMKRLEGGCSVPVAVNTLLKDGQLYLTGAVYSLDGSDSLKETMQTSVNYPQQNEDGPNDDVQHVGITAKNVPGQAQEAAEDLGVELASLLLSKGAKHILSVARQLNDAR

Target ORF information:

RefSeq Version	XM_009974849.1
Organism	Tyto alba(Barn owl)
Definition	Tyto alba hydroxymethylbilane synthase (HMBS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009974849.1

ORF Insert Sequence:

ATGATGTGCT CTTTGTTTAG GGCTTTGCTT TCTGCCTCGT TGCAGCTGGC CCGGATTCAG 
ACCGACAGCG TGGTCGAGAT GCTCCGTGAG CTATACCCCT ATCTGCGCTT TGAGATTGTT 
GCCATGTCAA CGACTGGAGA CAAGATCCTG GATACAGCGC TTTCCAAGAT TGGGGAGAAG 
AGCCTCTTCA CCAAAGAGCT GGAAAATGCC CTTGAAAGAA ATGAAGTTGA CCTTGTGGTT 
CACTCCTTGA AGGACCTGCC AACTTCTCTT CCTCCTGGCT TTACCATCGG TGCTGTCTGC 
AAAAGGGAAA ACCCGCTCGA TGCTGTCGTC TTTCATCCCA AAAACTGTGG GAAAACACTG 
AGCCTCCTTC CTGAAAAGAG TGTGATTGGG ACCAGTTCAC TTCGGAGAGC AGCCCAGCTC 
AAAAAGAAGT TCCCTCAGTT AGAATTCAGG GATATTAGAG GGAATTTAAA TACCCGCTTA 
AAGAAGCTGG ATGAGAAGGA AGACTTCAGT GCCATCGTTC TGGCTGCCGC TGGGCTGAAG 
AGGATGGGCT GGGAGGATCG CATTGGCCAG CTCCTAAGCC CTGAAGATTG TCTGTATGCT 
GTTGGACAGG GTGCCTTAGC AGTGGAAGTT CGTGCCAAAG ACCAAGAGAT ACTGAATATG 
GTATCTGCTC TGCACGACGC AGACACTGTG TTACGCTGCA TCGCCGAGAG AGCCTTTATG 
AAACGTCTGG AGGGTGGATG TAGCGTCCCT GTTGCAGTCA ACACTCTGCT GAAAGACGGC 
CAGTTGTATT TGACGGGAGC GGTCTACAGT TTGGACGGAT CCGATAGCCT GAAGGAGACT 
ATGCAGACCA GTGTTAATTA CCCCCAACAG AATGAAGATG GACCAAACGA CGATGTGCAG 
CACGTTGGCA TCACGGCCAA GAATGTCCCC GGTCAGGCCC AAGAAGCTGC AGAAGACCTT 
GGTGTTGAGC TAGCTAGTTT ACTTCTGAGT AAGGGAGCTA AGCATATCCT TAGCGTGGCA 
AGGCAGCTCA ACGATGCCCG CTAA