SHH cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHH
Entrez Gene ID	101327467
Full Name	sonic hedgehog
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019930219.1	XP_019785778.1	sonic hedgehog protein

Pathways from BioSystems
Gene Ontology

The following SHH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTu38319	XM_019930219.1 Latest version!	Tursiops truncatus sonic hedgehog (SHH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHH ( XM_019930219.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019785778.1 Tursiops truncatus sonic hedgehog (SHH), mRNA.

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CloneID	OTu38319
Clone ID Related Accession (Same CDS sequence)	XM_019930219.1
Accession Version	XM_019930219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	sonic hedgehog protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842806.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 30% of CDS bases ##RefSeq-Attributes-END##

ATGGAGGGCG AGAGGCCACA GCTGGCCCCG AAGGTGGATG CCGAGCTAGA GGGCGAAAGT 
GATGGAGAAG TTCACTGGCT GGGCCACGGG CTGACCATGG GTCCTGCGGG GACGGCAAAG 
GCGAAGGGGC CGCAGCACCG GGTCTCAACC CCCTGGCTGG AGACCGGGGA GCCCGTGTGG 
CCACCGCATG AGCAGGGATG CCCGCCCGAG ATCTCGCAGT GCGCGCGGGG CTGGGCTGCG 
GCCGCCCGCC GGCCCCTCCA CGCGCCCGAG CGCGGGCTCG GCCAGGGCTC CCGGCGGCGC 
TCCGACGGCC CGGCTGCCGA GGCGGCGCGG GCGGGCCCGG AGGGCCCGGG CGGGAGGGAG 
AAGCCGGTTC CAGGGCGGAG GCGCCTCGCG GGCCTGGGCG CCCGGCCCGA GCCCCCCGCC 
GGGTCCCTCG CCCCGCCGCC CCCGGCCGCC CCGCCGGGAC CTACAGCCCG GTGCAAGGAC 
AAGTTGAACG CCTTAGCCAT CTCCGTGATG AACCAGTGGC CAGGAGTGAA GCTGCGGGTG 
ACAGAGGGCT GGGATGAGGA CGGCCACCAC TCAGAGGAGT CGCTGCACTA CGAGGGCCGT 
GCAGTGGACA TCACCACCTC GGACCGCGAC CGCAGCAAGT ACGGCATGCT GGCACGCCTG 
GCTGTGGAGG CCGGCTTCGA CTGGGTCTAC TACGAGTCCA AAGCGCACAT CCACTGCTCG 
GTGAAAGCAG AGAACTCGGT GGCGGCCAAA TCGGGCGGCT GCTTCCCGGG CTCGGCCACG 
GTGCACCTGG AGCAGGGCGG CACCAAGCTG GTGAAGGACC TGCGCCCCGG AGACCGCGTG 
CTGGCTGCCG ACGACGAGGG CCGGCTGCTC TACAGCGACT TCCTCACCTT CCTGGACCGC 
GACCACGGCG CCAAGAAGGT CTTCTACGCG ATCGAGACCC GGGAGCCTCG CGAGCGTCTG 
CTGCTCACCG CCGCGCACCT GCTCTTTGTC GCGCCGCACA ACGGCTCGGC CGGCCGGGAG 
CCCGAGGCGC TGTCGGGCGC CGGGCGGCCG CCTGGGGGCG CGCCGGAGCG CCGGGCGCTC 
TTCGCCAGCA GCGTGCGCCC TGGCCAGCAC GTGTACGTGG TGGCCGAGCG CGGCGGGGAT 
CGGCGGCTCC TGCCGGCCGC CGTGCACAGC GTGACGCTGC GCGAGGAGAG CACGGGCGCG 
TACGCGCCGC TGACGGCGCA GGGCACCATC CTCATCAACC GGGTGCTGGC GTCGTGCTAC 
GCGGTCATAG AGGAGCACAA CTGGGCGCAC CGGGCCTTCG CGCCCTACCG CCTGGCGCAC 
GCGCTCCTGG CCGCGCTGGC GCCCTCGCGC ACGGACCGCG GCGGGGGCGG GGGCGGCAGC 
CGCGTGCCCC CGGCCGCGCC AGGTGCGCCG GCTGTGCCAG GTGCGACCGA CGCGCCAGGC 
GCCTCGGGCA TCCACTGGTA CTCGCAGCTG CTCTACCAAA TAGGCACCTG GCTGTTGGAC 
AGCGAGGCCT TGCACCCGCT CGGCATGGCG GTCAAGTCCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019785778.1
CDS	1..1545
Translation	MEGERPQLAPKVDAELEGESDGEVHWLGHGLTMGPAGTAKAKGPQHRVSTPWLETGEPVWPPHEQGCPPEISQCARGWAAAARRPLHAPERGLGQGSRRRSDGPAAEAARAGPEGPGGREKPVPGRRRLAGLGARPEPPAGSLAPPPPAAPPGPTARCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDEGRLLYSDFLTFLDRDHGAKKVFYAIETREPRERLLLTAAHLLFVAPHNGSAGREPEALSGAGRPPGGAPERRALFASSVRPGQHVYVVAERGGDRRLLPAAVHSVTLREESTGAYAPLTAQGTILINRVLASCYAVIEEHNWAHRAFAPYRLAHALLAALAPSRTDRGGGGGGSRVPPAAPGAPAVPGATDAPGASGIHWYSQLLYQIGTWLLDSEALHPLGMAVKSS

Target ORF information:

RefSeq Version	XM_019930219.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus sonic hedgehog (SHH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019930219.1

ORF Insert Sequence:

ATGGAGGGCG AGAGGCCACA GCTGGCCCCG AAGGTGGATG CCGAGCTAGA GGGCGAAAGT 
GATGGAGAAG TTCACTGGCT GGGCCACGGG CTGACCATGG GTCCTGCGGG GACGGCAAAG 
GCGAAGGGGC CGCAGCACCG GGTCTCAACC CCCTGGCTGG AGACCGGGGA GCCCGTGTGG 
CCACCGCATG AGCAGGGATG CCCGCCCGAG ATCTCGCAGT GCGCGCGGGG CTGGGCTGCG 
GCCGCCCGCC GGCCCCTCCA CGCGCCCGAG CGCGGGCTCG GCCAGGGCTC CCGGCGGCGC 
TCCGACGGCC CGGCTGCCGA GGCGGCGCGG GCGGGCCCGG AGGGCCCGGG CGGGAGGGAG 
AAGCCGGTTC CAGGGCGGAG GCGCCTCGCG GGCCTGGGCG CCCGGCCCGA GCCCCCCGCC 
GGGTCCCTCG CCCCGCCGCC CCCGGCCGCC CCGCCGGGAC CTACAGCCCG GTGCAAGGAC 
AAGTTGAACG CCTTAGCCAT CTCCGTGATG AACCAGTGGC CAGGAGTGAA GCTGCGGGTG 
ACAGAGGGCT GGGATGAGGA CGGCCACCAC TCAGAGGAGT CGCTGCACTA CGAGGGCCGT 
GCAGTGGACA TCACCACCTC GGACCGCGAC CGCAGCAAGT ACGGCATGCT GGCACGCCTG 
GCTGTGGAGG CCGGCTTCGA CTGGGTCTAC TACGAGTCCA AAGCGCACAT CCACTGCTCG 
GTGAAAGCAG AGAACTCGGT GGCGGCCAAA TCGGGCGGCT GCTTCCCGGG CTCGGCCACG 
GTGCACCTGG AGCAGGGCGG CACCAAGCTG GTGAAGGACC TGCGCCCCGG AGACCGCGTG 
CTGGCTGCCG ACGACGAGGG CCGGCTGCTC TACAGCGACT TCCTCACCTT CCTGGACCGC 
GACCACGGCG CCAAGAAGGT CTTCTACGCG ATCGAGACCC GGGAGCCTCG CGAGCGTCTG 
CTGCTCACCG CCGCGCACCT GCTCTTTGTC GCGCCGCACA ACGGCTCGGC CGGCCGGGAG 
CCCGAGGCGC TGTCGGGCGC CGGGCGGCCG CCTGGGGGCG CGCCGGAGCG CCGGGCGCTC 
TTCGCCAGCA GCGTGCGCCC TGGCCAGCAC GTGTACGTGG TGGCCGAGCG CGGCGGGGAT 
CGGCGGCTCC TGCCGGCCGC CGTGCACAGC GTGACGCTGC GCGAGGAGAG CACGGGCGCG 
TACGCGCCGC TGACGGCGCA GGGCACCATC CTCATCAACC GGGTGCTGGC GTCGTGCTAC 
GCGGTCATAG AGGAGCACAA CTGGGCGCAC CGGGCCTTCG CGCCCTACCG CCTGGCGCAC 
GCGCTCCTGG CCGCGCTGGC GCCCTCGCGC ACGGACCGCG GCGGGGGCGG GGGCGGCAGC 
CGCGTGCCCC CGGCCGCGCC AGGTGCGCCG GCTGTGCCAG GTGCGACCGA CGCGCCAGGC 
GCCTCGGGCA TCCACTGGTA CTCGCAGCTG CTCTACCAAA TAGGCACCTG GCTGTTGGAC 
AGCGAGGCCT TGCACCCGCT CGGCATGGCG GTCAAGTCCA GCTGA