PLCL2 cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCL2
Entrez Gene ID	101315838
Full Name	phospholipase C like 2
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019934417.1	XP_019789976.1	inactive phospholipase C-like protein 2 isoform X1
XM_019934418.1	XP_019789977.1	inactive phospholipase C-like protein 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following PLCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTu41783	XM_019934418.1 Latest version!	Tursiops truncatus phospholipase C like 2 (PLCL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTu41782	XM_019934417.1 Latest version!	Tursiops truncatus phospholipase C like 2 (PLCL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCL2 ( XM_019934418.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019789977.1 Tursiops truncatus phospholipase C like 2 (PLCL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTu41783
Clone ID Related Accession (Same CDS sequence)	XM_019934418.1
Accession Version	XM_019934418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	inactive phospholipase C-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843160.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACGG AGAAAAAGAT TAGCAGTGCA AGTGATTGTA TCAATTCCAT GGTGGAGGGT 
TCGGAACTCA AAAAGGTTCG CTCCAACTCT AGAATTTACC ACAGGTATTT TTTACTGGAC 
GCTGACATGC AGAGCCTGAG GTGGGAGCCA TCCAAGAAGG ATTCTGAGAA AGCCAAGATT 
GATATTAAAT CCATCAAGGA AGTGAGAACA GGAAAAAATA CAGACATATT CCGCAGCAAT 
GGCATTTCTG ACCAGATATC TGAAGACTGT GCATTTTCCG TCATCTACGG AGAGAATTAC 
GAGTCACTTG ATTTGGTTGC CAACTCTGCA GATGTTGCGA ACATCTGGGT CACGGGACTG 
CGGTACCTAA TTTCTTATGG GAAACATACG CTCGATATGT TAGAGAGTAG CCAAGACAAC 
ATGAGGACTT CTTGGGTCTC ACAAATGTTT AGTGAAATTG ATGTAGATAA CCTGGGACAT 
ATAACTCTGT GCAGCGCCGT ACAGTGTATC AGAAATCTCA ATCCTGGTTT AAAAACGAGC 
AAGATTGAGC TTAAGTTCAA AGAATTGCAC AAGTCCAAGG ACAAAGCTGG TACTGAAGTC 
ACAAAGGAGG AATTTGTTGA GGTTTTCCAC GAGCTTTGTA CTAGACCTGA AATTTATTTC 
CTTTTAGTTC AGTTCTCAAG CAATAAAGAA TTCCTTGATA CCAAGGACCT TATGATGTTT 
CTGGAGGCAG AGCAGGGTGT GGCACATATA AATGAGGAAA TAAGCCTTGA AATTATTCAC 
AAATATGAGC CATCCAAAGA GGGTCAGGAA AAGGGCTGGC TGTCCATAGA CGGGTTCACT 
AATTACCTTG TGTCCTCTGA CTGTTATATA TTTGATCCAG AGCATAAGAA GGTCTGTCAG 
GACATGAAGC AACCCTTGTC TCATTACTTT ATAAACTCAT CTCATAATAC GTACTTAATA 
GAGGATCAGT TCCGAGGTCC CTCGGATATC ACAGGATACA TCCGAGCTCT TAAAATGGGT 
TGCCGGAGTG TTGAATTGGA TGTATGGGAC GGGCCGGATA ATGAACCTGT GATTTACACA 
GGCCACACCA TGACCTCGCA GATCGTCTTC CGCAGCGTCA TTGATATTAT TAACAAGTAT 
GCATTCTTTG CTTCAGAGTA CCCTCTCATC CTGTGTTTGG AAAATCACTG TTCTATTAAA 
CAACAGAAGG TCATGGTTCA ACACATGAAG AAAATTTTAG GAGACAAGCT CTACACAACC 
TCACCCAGTG CTGAGGAATC TTACCTGCCA TCCCCAGATG TCCTGAAAGG GAAGATACTC 
ATTAAGGCAA AGAAGCTGTC TTCCAACTGC TCTGGAGTAG AGGGCGATGT TACTGATGAG 
GATGAAGGGG CAGAGATGTC TCAGAGGATG GGCAAGGAGA ACGCAGAGCT GCCCAACCAC 
GTGCCTGCCA AGCGCTTCCA GCTCTGCAAA GAGCTGTCCG AGCTGGTGAG CATCTGCAAG 
TCGGTGCAGT TCAAAGAGTT CCAGGTGTCC TTTCAGGTTC AGAAGTACTG GGAAGTCTGC 
TCGTTCAACG AAGTGCTTGC CAGCAAATAC GCCAACGAGA ACGCAGGGGA CTTTGTCAAT 
TATAACAAGC GCTTTCTCGC CAGGGTTTTT CCCAGCCCCA TGAGAATTGA CTCCAGCAAC 
ATGAACCCTC AAGATTTTTG GAAATGTGGT TGTCAGATCG TGGCCATGAA CTTTCAGACC 
CCGGGACTGA TGATGGACCT GAACATCGGC TGGTTTAGGC AGAACGGGAA CTGCGGCTAC 
GTGCTGCGGC CGGCCATCAT GAGGGAGGAG GTCTCCTTCT TCAGCGCCAA CACCAAAGAC 
TCCGTCCCAG GAGTCTCACC CCAGCTCCTT CACATCAAGA TCATCAGCGG GCAGAACTTC 
CCGAAACCCA AAGGATCGGG TGCTAAAGGC GACGTGGTGG ACCCTTACGT CTACGTTGAG 
ATCCACGGGA TCCCCGCTGA CTGTGCAGAA CAGAGGACAA AAACAGTGCA CCAGAACGGA 
GACGCTCCCA TCTTCGACGA AAGCTTTGAG TTCCAGATCA ACCTGCCCGA ACTGGCCATG 
GTGCGCTTTG TGGTCCTGGA CGACGACTAC ATCGGGGATG AGTTCATCGG CCAGTACACG 
ATTCCCTTCG AGTGTCTGCA GACGGGCTAC CGCCACGTGC CGCTGCAGTC GCTCACCGGC 
GAGGTCCTCG CCCACGCTTC TCTGTTCGTG CACGTGGCCA TCACGAACCG GAGAGGAGGC 
GGGAAGCCTC ACAAGAGGGG CCTCTCTGTG AGAAAAGGGA AGAAGTCCAG GGAATATGCG 
TCCCTGAGAA CACTGTGGAT TAAAACTGTG GACGAGGTCT TCAAGAATGC CCAGTCCCCA 
ATTCGGGACG CCACCGACCT GAGAGAGAAC ATGCAGAATG CAGTGGTGTC CTTCAAGGAG 
CTGTGTGGCC TCTCCTCCGT AGCCAACCTC ATGCAGTGCA TGCTGGCGGT GTCTCCCCGC 
TTCCTGGGAC CTGATAACAC GCCCCTGGTG GTCCTGAATC TCAGCGAGCA GTATCCCACC 
ATGGAGTTGC AGGGAATCGT GCCCGAGGTT CTGAAAAAGA TCGTAACAAC TTACGACATG 
ATGATCCAGT CCCTCAAGGC GTTGATTGAA AATGCAGACG CTGTGTATGA AAAGATTGTA 
CACTGTCAGA AAGCAGCCAT GGAATTCCAT GAACACTTGC ACAGCATAGG CACCAAGGAA 
GGTCTGAAGG AACGCAAACT GCAAAAAGCT GTGGAGAGCT TTACCTGGAA TATTACCATC 
TTAAAGGGAC AAGCGGATCT CTTGAAGTAC GCCAAGAACG AGACCGTGGA GAACCTGAAG 
CAGATCCACT ATGCGGCTGT CTCCTGTGGA CTCAATAAAC CAGGCACAGA AAACACCGAC 
GTCCAGAAGC CGCGCCGGAG CCTTGAAGTC ATACCCGAAA AAGCAAACGA TGAAACTGGA 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019789977.1
CDS	355..3360
Translation	MPTEKKISSASDCINSMVEGSELKKVRSNSRIYHRYFLLDADMQSLRWEPSKKDSEKAKIDIKSIKEVRTGKNTDIFRSNGISDQISEDCAFSVIYGENYESLDLVANSADVANIWVTGLRYLISYGKHTLDMLESSQDNMRTSWVSQMFSEIDVDNLGHITLCSAVQCIRNLNPGLKTSKIELKFKELHKSKDKAGTEVTKEEFVEVFHELCTRPEIYFLLVQFSSNKEFLDTKDLMMFLEAEQGVAHINEEISLEIIHKYEPSKEGQEKGWLSIDGFTNYLVSSDCYIFDPEHKKVCQDMKQPLSHYFINSSHNTYLIEDQFRGPSDITGYIRALKMGCRSVELDVWDGPDNEPVIYTGHTMTSQIVFRSVIDIINKYAFFASEYPLILCLENHCSIKQQKVMVQHMKKILGDKLYTTSPSAEESYLPSPDVLKGKILIKAKKLSSNCSGVEGDVTDEDEGAEMSQRMGKENAELPNHVPAKRFQLCKELSELVSICKSVQFKEFQVSFQVQKYWEVCSFNEVLASKYANENAGDFVNYNKRFLARVFPSPMRIDSSNMNPQDFWKCGCQIVAMNFQTPGLMMDLNIGWFRQNGNCGYVLRPAIMREEVSFFSANTKDSVPGVSPQLLHIKIISGQNFPKPKGSGAKGDVVDPYVYVEIHGIPADCAEQRTKTVHQNGDAPIFDESFEFQINLPELAMVRFVVLDDDYIGDEFIGQYTIPFECLQTGYRHVPLQSLTGEVLAHASLFVHVAITNRRGGGKPHKRGLSVRKGKKSREYASLRTLWIKTVDEVFKNAQSPIRDATDLRENMQNAVVSFKELCGLSSVANLMQCMLAVSPRFLGPDNTPLVVLNLSEQYPTMELQGIVPEVLKKIVTTYDMMIQSLKALIENADAVYEKIVHCQKAAMEFHEHLHSIGTKEGLKERKLQKAVESFTWNITILKGQADLLKYAKNETVENLKQIHYAAVSCGLNKPGTENTDVQKPRRSLEVIPEKANDETGE

Target ORF information:

RefSeq Version	XM_019934418.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus phospholipase C like 2 (PLCL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019934418.1

ORF Insert Sequence:

ATGCCCACGG AGAAAAAGAT TAGCAGTGCA AGTGATTGTA TCAATTCCAT GGTGGAGGGT 
TCGGAACTCA AAAAGGTTCG CTCCAACTCT AGAATTTACC ACAGGTATTT TTTACTGGAC 
GCTGACATGC AGAGCCTGAG GTGGGAGCCA TCCAAGAAGG ATTCTGAGAA AGCCAAGATT 
GATATTAAAT CCATCAAGGA AGTGAGAACA GGAAAAAATA CAGACATATT CCGCAGCAAT 
GGCATTTCTG ACCAGATATC TGAAGACTGT GCATTTTCCG TCATCTACGG AGAGAATTAC 
GAGTCACTTG ATTTGGTTGC CAACTCTGCA GATGTTGCGA ACATCTGGGT CACGGGACTG 
CGGTACCTAA TTTCTTATGG GAAACATACG CTCGATATGT TAGAGAGTAG CCAAGACAAC 
ATGAGGACTT CTTGGGTCTC ACAAATGTTT AGTGAAATTG ATGTAGATAA CCTGGGACAT 
ATAACTCTGT GCAGCGCCGT ACAGTGTATC AGAAATCTCA ATCCTGGTTT AAAAACGAGC 
AAGATTGAGC TTAAGTTCAA AGAATTGCAC AAGTCCAAGG ACAAAGCTGG TACTGAAGTC 
ACAAAGGAGG AATTTGTTGA GGTTTTCCAC GAGCTTTGTA CTAGACCTGA AATTTATTTC 
CTTTTAGTTC AGTTCTCAAG CAATAAAGAA TTCCTTGATA CCAAGGACCT TATGATGTTT 
CTGGAGGCAG AGCAGGGTGT GGCACATATA AATGAGGAAA TAAGCCTTGA AATTATTCAC 
AAATATGAGC CATCCAAAGA GGGTCAGGAA AAGGGCTGGC TGTCCATAGA CGGGTTCACT 
AATTACCTTG TGTCCTCTGA CTGTTATATA TTTGATCCAG AGCATAAGAA GGTCTGTCAG 
GACATGAAGC AACCCTTGTC TCATTACTTT ATAAACTCAT CTCATAATAC GTACTTAATA 
GAGGATCAGT TCCGAGGTCC CTCGGATATC ACAGGATACA TCCGAGCTCT TAAAATGGGT 
TGCCGGAGTG TTGAATTGGA TGTATGGGAC GGGCCGGATA ATGAACCTGT GATTTACACA 
GGCCACACCA TGACCTCGCA GATCGTCTTC CGCAGCGTCA TTGATATTAT TAACAAGTAT 
GCATTCTTTG CTTCAGAGTA CCCTCTCATC CTGTGTTTGG AAAATCACTG TTCTATTAAA 
CAACAGAAGG TCATGGTTCA ACACATGAAG AAAATTTTAG GAGACAAGCT CTACACAACC 
TCACCCAGTG CTGAGGAATC TTACCTGCCA TCCCCAGATG TCCTGAAAGG GAAGATACTC 
ATTAAGGCAA AGAAGCTGTC TTCCAACTGC TCTGGAGTAG AGGGCGATGT TACTGATGAG 
GATGAAGGGG CAGAGATGTC TCAGAGGATG GGCAAGGAGA ACGCAGAGCT GCCCAACCAC 
GTGCCTGCCA AGCGCTTCCA GCTCTGCAAA GAGCTGTCCG AGCTGGTGAG CATCTGCAAG 
TCGGTGCAGT TCAAAGAGTT CCAGGTGTCC TTTCAGGTTC AGAAGTACTG GGAAGTCTGC 
TCGTTCAACG AAGTGCTTGC CAGCAAATAC GCCAACGAGA ACGCAGGGGA CTTTGTCAAT 
TATAACAAGC GCTTTCTCGC CAGGGTTTTT CCCAGCCCCA TGAGAATTGA CTCCAGCAAC 
ATGAACCCTC AAGATTTTTG GAAATGTGGT TGTCAGATCG TGGCCATGAA CTTTCAGACC 
CCGGGACTGA TGATGGACCT GAACATCGGC TGGTTTAGGC AGAACGGGAA CTGCGGCTAC 
GTGCTGCGGC CGGCCATCAT GAGGGAGGAG GTCTCCTTCT TCAGCGCCAA CACCAAAGAC 
TCCGTCCCAG GAGTCTCACC CCAGCTCCTT CACATCAAGA TCATCAGCGG GCAGAACTTC 
CCGAAACCCA AAGGATCGGG TGCTAAAGGC GACGTGGTGG ACCCTTACGT CTACGTTGAG 
ATCCACGGGA TCCCCGCTGA CTGTGCAGAA CAGAGGACAA AAACAGTGCA CCAGAACGGA 
GACGCTCCCA TCTTCGACGA AAGCTTTGAG TTCCAGATCA ACCTGCCCGA ACTGGCCATG 
GTGCGCTTTG TGGTCCTGGA CGACGACTAC ATCGGGGATG AGTTCATCGG CCAGTACACG 
ATTCCCTTCG AGTGTCTGCA GACGGGCTAC CGCCACGTGC CGCTGCAGTC GCTCACCGGC 
GAGGTCCTCG CCCACGCTTC TCTGTTCGTG CACGTGGCCA TCACGAACCG GAGAGGAGGC 
GGGAAGCCTC ACAAGAGGGG CCTCTCTGTG AGAAAAGGGA AGAAGTCCAG GGAATATGCG 
TCCCTGAGAA CACTGTGGAT TAAAACTGTG GACGAGGTCT TCAAGAATGC CCAGTCCCCA 
ATTCGGGACG CCACCGACCT GAGAGAGAAC ATGCAGAATG CAGTGGTGTC CTTCAAGGAG 
CTGTGTGGCC TCTCCTCCGT AGCCAACCTC ATGCAGTGCA TGCTGGCGGT GTCTCCCCGC 
TTCCTGGGAC CTGATAACAC GCCCCTGGTG GTCCTGAATC TCAGCGAGCA GTATCCCACC 
ATGGAGTTGC AGGGAATCGT GCCCGAGGTT CTGAAAAAGA TCGTAACAAC TTACGACATG 
ATGATCCAGT CCCTCAAGGC GTTGATTGAA AATGCAGACG CTGTGTATGA AAAGATTGTA 
CACTGTCAGA AAGCAGCCAT GGAATTCCAT GAACACTTGC ACAGCATAGG CACCAAGGAA 
GGTCTGAAGG AACGCAAACT GCAAAAAGCT GTGGAGAGCT TTACCTGGAA TATTACCATC 
TTAAAGGGAC AAGCGGATCT CTTGAAGTAC GCCAAGAACG AGACCGTGGA GAACCTGAAG 
CAGATCCACT ATGCGGCTGT CTCCTGTGGA CTCAATAAAC CAGGCACAGA AAACACCGAC 
GTCCAGAAGC CGCGCCGGAG CCTTGAAGTC ATACCCGAAA AAGCAAACGA TGAAACTGGA 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL2 ( XM_019934417.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019789976.1 Tursiops truncatus phospholipase C like 2 (PLCL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTu41782
Clone ID Related Accession (Same CDS sequence)	XM_019934417.1
Accession Version	XM_019934417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	inactive phospholipase C-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843160.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGT GCGGCCGGGG GGGCGCCGCC GGCGGGGCCC TGCCCACCTC CCCGGGCCCG 
GTCCTCGCGG CCAAGGGCGC CCTGAGAGCC GGGGCCGGGG AAGGCGGCGG CGGCGGCGGA 
GGGCGCCTCG GCCACGGGCG GGCGCGCTAT GACAGCGCCG GGGTTTCCAA CGGAGACTGC 
AGCCTCGGCG TGTCCGCGGA CGAAGCCCAG GCCAGCCCCT CCAGGGGCCC CCGCGGCGCC 
GCGCTCGCCC CGACCCCCGG CCCGGCCGCC TGCCCCCTCC CCCGGGAGAG CAAGCCGGGC 
GGCCTGCCCC GCCGGAGCAG CATCATCAAG GATGGTACAA AACAGAAAAG AGAGCGGAAA 
AAGACGGTCT CCTTCAGCAG TATGCCCACG GAGAAAAAGA TTAGCAGTGC AAGTGATTGT 
ATCAATTCCA TGGTGGAGGG TTCGGAACTC AAAAAGGTTC GCTCCAACTC TAGAATTTAC 
CACAGGTATT TTTTACTGGA CGCTGACATG CAGAGCCTGA GGTGGGAGCC ATCCAAGAAG 
GATTCTGAGA AAGCCAAGAT TGATATTAAA TCCATCAAGG AAGTGAGAAC AGGAAAAAAT 
ACAGACATAT TCCGCAGCAA TGGCATTTCT GACCAGATAT CTGAAGACTG TGCATTTTCC 
GTCATCTACG GAGAGAATTA CGAGTCACTT GATTTGGTTG CCAACTCTGC AGATGTTGCG 
AACATCTGGG TCACGGGACT GCGGTACCTA ATTTCTTATG GGAAACATAC GCTCGATATG 
TTAGAGAGTA GCCAAGACAA CATGAGGACT TCTTGGGTCT CACAAATGTT TAGTGAAATT 
GATGTAGATA ACCTGGGACA TATAACTCTG TGCAGCGCCG TACAGTGTAT CAGAAATCTC 
AATCCTGGTT TAAAAACGAG CAAGATTGAG CTTAAGTTCA AAGAATTGCA CAAGTCCAAG 
GACAAAGCTG GTACTGAAGT CACAAAGGAG GAATTTGTTG AGGTTTTCCA CGAGCTTTGT 
ACTAGACCTG AAATTTATTT CCTTTTAGTT CAGTTCTCAA GCAATAAAGA ATTCCTTGAT 
ACCAAGGACC TTATGATGTT TCTGGAGGCA GAGCAGGGTG TGGCACATAT AAATGAGGAA 
ATAAGCCTTG AAATTATTCA CAAATATGAG CCATCCAAAG AGGGTCAGGA AAAGGGCTGG 
CTGTCCATAG ACGGGTTCAC TAATTACCTT GTGTCCTCTG ACTGTTATAT ATTTGATCCA 
GAGCATAAGA AGGTCTGTCA GGACATGAAG CAACCCTTGT CTCATTACTT TATAAACTCA 
TCTCATAATA CGTACTTAAT AGAGGATCAG TTCCGAGGTC CCTCGGATAT CACAGGATAC 
ATCCGAGCTC TTAAAATGGG TTGCCGGAGT GTTGAATTGG ATGTATGGGA CGGGCCGGAT 
AATGAACCTG TGATTTACAC AGGCCACACC ATGACCTCGC AGATCGTCTT CCGCAGCGTC 
ATTGATATTA TTAACAAGTA TGCATTCTTT GCTTCAGAGT ACCCTCTCAT CCTGTGTTTG 
GAAAATCACT GTTCTATTAA ACAACAGAAG GTCATGGTTC AACACATGAA GAAAATTTTA 
GGAGACAAGC TCTACACAAC CTCACCCAGT GCTGAGGAAT CTTACCTGCC ATCCCCAGAT 
GTCCTGAAAG GGAAGATACT CATTAAGGCA AAGAAGCTGT CTTCCAACTG CTCTGGAGTA 
GAGGGCGATG TTACTGATGA GGATGAAGGG GCAGAGATGT CTCAGAGGAT GGGCAAGGAG 
AACGCAGAGC TGCCCAACCA CGTGCCTGCC AAGCGCTTCC AGCTCTGCAA AGAGCTGTCC 
GAGCTGGTGA GCATCTGCAA GTCGGTGCAG TTCAAAGAGT TCCAGGTGTC CTTTCAGGTT 
CAGAAGTACT GGGAAGTCTG CTCGTTCAAC GAAGTGCTTG CCAGCAAATA CGCCAACGAG 
AACGCAGGGG ACTTTGTCAA TTATAACAAG CGCTTTCTCG CCAGGGTTTT TCCCAGCCCC 
ATGAGAATTG ACTCCAGCAA CATGAACCCT CAAGATTTTT GGAAATGTGG TTGTCAGATC 
GTGGCCATGA ACTTTCAGAC CCCGGGACTG ATGATGGACC TGAACATCGG CTGGTTTAGG 
CAGAACGGGA ACTGCGGCTA CGTGCTGCGG CCGGCCATCA TGAGGGAGGA GGTCTCCTTC 
TTCAGCGCCA ACACCAAAGA CTCCGTCCCA GGAGTCTCAC CCCAGCTCCT TCACATCAAG 
ATCATCAGCG GGCAGAACTT CCCGAAACCC AAAGGATCGG GTGCTAAAGG CGACGTGGTG 
GACCCTTACG TCTACGTTGA GATCCACGGG ATCCCCGCTG ACTGTGCAGA ACAGAGGACA 
AAAACAGTGC ACCAGAACGG AGACGCTCCC ATCTTCGACG AAAGCTTTGA GTTCCAGATC 
AACCTGCCCG AACTGGCCAT GGTGCGCTTT GTGGTCCTGG ACGACGACTA CATCGGGGAT 
GAGTTCATCG GCCAGTACAC GATTCCCTTC GAGTGTCTGC AGACGGGCTA CCGCCACGTG 
CCGCTGCAGT CGCTCACCGG CGAGGTCCTC GCCCACGCTT CTCTGTTCGT GCACGTGGCC 
ATCACGAACC GGAGAGGAGG CGGGAAGCCT CACAAGAGGG GCCTCTCTGT GAGAAAAGGG 
AAGAAGTCCA GGGAATATGC GTCCCTGAGA ACACTGTGGA TTAAAACTGT GGACGAGGTC 
TTCAAGAATG CCCAGTCCCC AATTCGGGAC GCCACCGACC TGAGAGAGAA CATGCAGAAT 
GCAGTGGTGT CCTTCAAGGA GCTGTGTGGC CTCTCCTCCG TAGCCAACCT CATGCAGTGC 
ATGCTGGCGG TGTCTCCCCG CTTCCTGGGA CCTGATAACA CGCCCCTGGT GGTCCTGAAT 
CTCAGCGAGC AGTATCCCAC CATGGAGTTG CAGGGAATCG TGCCCGAGGT TCTGAAAAAG 
ATCGTAACAA CTTACGACAT GATGATCCAG TCCCTCAAGG CGTTGATTGA AAATGCAGAC 
GCTGTGTATG AAAAGATTGT ACACTGTCAG AAAGCAGCCA TGGAATTCCA TGAACACTTG 
CACAGCATAG GCACCAAGGA AGGTCTGAAG GAACGCAAAC TGCAAAAAGC TGTGGAGAGC 
TTTACCTGGA ATATTACCAT CTTAAAGGGA CAAGCGGATC TCTTGAAGTA CGCCAAGAAC 
GAGACCGTGG AGAACCTGAA GCAGATCCAC TATGCGGCTG TCTCCTGTGG ACTCAATAAA 
CCAGGCACAG AAAACACCGA CGTCCAGAAG CCGCGCCGGA GCCTTGAAGT CATACCCGAA 
AAAGCAAACG ATGAAACTGG AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019789976.1
CDS	64..3450
Translation	MAECGRGGAAGGALPTSPGPVLAAKGALRAGAGEGGGGGGGRLGHGRARYDSAGVSNGDCSLGVSADEAQASPSRGPRGAALAPTPGPAACPLPRESKPGGLPRRSSIIKDGTKQKRERKKTVSFSSMPTEKKISSASDCINSMVEGSELKKVRSNSRIYHRYFLLDADMQSLRWEPSKKDSEKAKIDIKSIKEVRTGKNTDIFRSNGISDQISEDCAFSVIYGENYESLDLVANSADVANIWVTGLRYLISYGKHTLDMLESSQDNMRTSWVSQMFSEIDVDNLGHITLCSAVQCIRNLNPGLKTSKIELKFKELHKSKDKAGTEVTKEEFVEVFHELCTRPEIYFLLVQFSSNKEFLDTKDLMMFLEAEQGVAHINEEISLEIIHKYEPSKEGQEKGWLSIDGFTNYLVSSDCYIFDPEHKKVCQDMKQPLSHYFINSSHNTYLIEDQFRGPSDITGYIRALKMGCRSVELDVWDGPDNEPVIYTGHTMTSQIVFRSVIDIINKYAFFASEYPLILCLENHCSIKQQKVMVQHMKKILGDKLYTTSPSAEESYLPSPDVLKGKILIKAKKLSSNCSGVEGDVTDEDEGAEMSQRMGKENAELPNHVPAKRFQLCKELSELVSICKSVQFKEFQVSFQVQKYWEVCSFNEVLASKYANENAGDFVNYNKRFLARVFPSPMRIDSSNMNPQDFWKCGCQIVAMNFQTPGLMMDLNIGWFRQNGNCGYVLRPAIMREEVSFFSANTKDSVPGVSPQLLHIKIISGQNFPKPKGSGAKGDVVDPYVYVEIHGIPADCAEQRTKTVHQNGDAPIFDESFEFQINLPELAMVRFVVLDDDYIGDEFIGQYTIPFECLQTGYRHVPLQSLTGEVLAHASLFVHVAITNRRGGGKPHKRGLSVRKGKKSREYASLRTLWIKTVDEVFKNAQSPIRDATDLRENMQNAVVSFKELCGLSSVANLMQCMLAVSPRFLGPDNTPLVVLNLSEQYPTMELQGIVPEVLKKIVTTYDMMIQSLKALIENADAVYEKIVHCQKAAMEFHEHLHSIGTKEGLKERKLQKAVESFTWNITILKGQADLLKYAKNETVENLKQIHYAAVSCGLNKPGTENTDVQKPRRSLEVIPEKANDETGE

Target ORF information:

RefSeq Version	XM_019934417.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus phospholipase C like 2 (PLCL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019934417.1

ORF Insert Sequence:

ATGGCGGAGT GCGGCCGGGG GGGCGCCGCC GGCGGGGCCC TGCCCACCTC CCCGGGCCCG 
GTCCTCGCGG CCAAGGGCGC CCTGAGAGCC GGGGCCGGGG AAGGCGGCGG CGGCGGCGGA 
GGGCGCCTCG GCCACGGGCG GGCGCGCTAT GACAGCGCCG GGGTTTCCAA CGGAGACTGC 
AGCCTCGGCG TGTCCGCGGA CGAAGCCCAG GCCAGCCCCT CCAGGGGCCC CCGCGGCGCC 
GCGCTCGCCC CGACCCCCGG CCCGGCCGCC TGCCCCCTCC CCCGGGAGAG CAAGCCGGGC 
GGCCTGCCCC GCCGGAGCAG CATCATCAAG GATGGTACAA AACAGAAAAG AGAGCGGAAA 
AAGACGGTCT CCTTCAGCAG TATGCCCACG GAGAAAAAGA TTAGCAGTGC AAGTGATTGT 
ATCAATTCCA TGGTGGAGGG TTCGGAACTC AAAAAGGTTC GCTCCAACTC TAGAATTTAC 
CACAGGTATT TTTTACTGGA CGCTGACATG CAGAGCCTGA GGTGGGAGCC ATCCAAGAAG 
GATTCTGAGA AAGCCAAGAT TGATATTAAA TCCATCAAGG AAGTGAGAAC AGGAAAAAAT 
ACAGACATAT TCCGCAGCAA TGGCATTTCT GACCAGATAT CTGAAGACTG TGCATTTTCC 
GTCATCTACG GAGAGAATTA CGAGTCACTT GATTTGGTTG CCAACTCTGC AGATGTTGCG 
AACATCTGGG TCACGGGACT GCGGTACCTA ATTTCTTATG GGAAACATAC GCTCGATATG 
TTAGAGAGTA GCCAAGACAA CATGAGGACT TCTTGGGTCT CACAAATGTT TAGTGAAATT 
GATGTAGATA ACCTGGGACA TATAACTCTG TGCAGCGCCG TACAGTGTAT CAGAAATCTC 
AATCCTGGTT TAAAAACGAG CAAGATTGAG CTTAAGTTCA AAGAATTGCA CAAGTCCAAG 
GACAAAGCTG GTACTGAAGT CACAAAGGAG GAATTTGTTG AGGTTTTCCA CGAGCTTTGT 
ACTAGACCTG AAATTTATTT CCTTTTAGTT CAGTTCTCAA GCAATAAAGA ATTCCTTGAT 
ACCAAGGACC TTATGATGTT TCTGGAGGCA GAGCAGGGTG TGGCACATAT AAATGAGGAA 
ATAAGCCTTG AAATTATTCA CAAATATGAG CCATCCAAAG AGGGTCAGGA AAAGGGCTGG 
CTGTCCATAG ACGGGTTCAC TAATTACCTT GTGTCCTCTG ACTGTTATAT ATTTGATCCA 
GAGCATAAGA AGGTCTGTCA GGACATGAAG CAACCCTTGT CTCATTACTT TATAAACTCA 
TCTCATAATA CGTACTTAAT AGAGGATCAG TTCCGAGGTC CCTCGGATAT CACAGGATAC 
ATCCGAGCTC TTAAAATGGG TTGCCGGAGT GTTGAATTGG ATGTATGGGA CGGGCCGGAT 
AATGAACCTG TGATTTACAC AGGCCACACC ATGACCTCGC AGATCGTCTT CCGCAGCGTC 
ATTGATATTA TTAACAAGTA TGCATTCTTT GCTTCAGAGT ACCCTCTCAT CCTGTGTTTG 
GAAAATCACT GTTCTATTAA ACAACAGAAG GTCATGGTTC AACACATGAA GAAAATTTTA 
GGAGACAAGC TCTACACAAC CTCACCCAGT GCTGAGGAAT CTTACCTGCC ATCCCCAGAT 
GTCCTGAAAG GGAAGATACT CATTAAGGCA AAGAAGCTGT CTTCCAACTG CTCTGGAGTA 
GAGGGCGATG TTACTGATGA GGATGAAGGG GCAGAGATGT CTCAGAGGAT GGGCAAGGAG 
AACGCAGAGC TGCCCAACCA CGTGCCTGCC AAGCGCTTCC AGCTCTGCAA AGAGCTGTCC 
GAGCTGGTGA GCATCTGCAA GTCGGTGCAG TTCAAAGAGT TCCAGGTGTC CTTTCAGGTT 
CAGAAGTACT GGGAAGTCTG CTCGTTCAAC GAAGTGCTTG CCAGCAAATA CGCCAACGAG 
AACGCAGGGG ACTTTGTCAA TTATAACAAG CGCTTTCTCG CCAGGGTTTT TCCCAGCCCC 
ATGAGAATTG ACTCCAGCAA CATGAACCCT CAAGATTTTT GGAAATGTGG TTGTCAGATC 
GTGGCCATGA ACTTTCAGAC CCCGGGACTG ATGATGGACC TGAACATCGG CTGGTTTAGG 
CAGAACGGGA ACTGCGGCTA CGTGCTGCGG CCGGCCATCA TGAGGGAGGA GGTCTCCTTC 
TTCAGCGCCA ACACCAAAGA CTCCGTCCCA GGAGTCTCAC CCCAGCTCCT TCACATCAAG 
ATCATCAGCG GGCAGAACTT CCCGAAACCC AAAGGATCGG GTGCTAAAGG CGACGTGGTG 
GACCCTTACG TCTACGTTGA GATCCACGGG ATCCCCGCTG ACTGTGCAGA ACAGAGGACA 
AAAACAGTGC ACCAGAACGG AGACGCTCCC ATCTTCGACG AAAGCTTTGA GTTCCAGATC 
AACCTGCCCG AACTGGCCAT GGTGCGCTTT GTGGTCCTGG ACGACGACTA CATCGGGGAT 
GAGTTCATCG GCCAGTACAC GATTCCCTTC GAGTGTCTGC AGACGGGCTA CCGCCACGTG 
CCGCTGCAGT CGCTCACCGG CGAGGTCCTC GCCCACGCTT CTCTGTTCGT GCACGTGGCC 
ATCACGAACC GGAGAGGAGG CGGGAAGCCT CACAAGAGGG GCCTCTCTGT GAGAAAAGGG 
AAGAAGTCCA GGGAATATGC GTCCCTGAGA ACACTGTGGA TTAAAACTGT GGACGAGGTC 
TTCAAGAATG CCCAGTCCCC AATTCGGGAC GCCACCGACC TGAGAGAGAA CATGCAGAAT 
GCAGTGGTGT CCTTCAAGGA GCTGTGTGGC CTCTCCTCCG TAGCCAACCT CATGCAGTGC 
ATGCTGGCGG TGTCTCCCCG CTTCCTGGGA CCTGATAACA CGCCCCTGGT GGTCCTGAAT 
CTCAGCGAGC AGTATCCCAC CATGGAGTTG CAGGGAATCG TGCCCGAGGT TCTGAAAAAG 
ATCGTAACAA CTTACGACAT GATGATCCAG TCCCTCAAGG CGTTGATTGA AAATGCAGAC 
GCTGTGTATG AAAAGATTGT ACACTGTCAG AAAGCAGCCA TGGAATTCCA TGAACACTTG 
CACAGCATAG GCACCAAGGA AGGTCTGAAG GAACGCAAAC TGCAAAAAGC TGTGGAGAGC 
TTTACCTGGA ATATTACCAT CTTAAAGGGA CAAGCGGATC TCTTGAAGTA CGCCAAGAAC 
GAGACCGTGG AGAACCTGAA GCAGATCCAC TATGCGGCTG TCTCCTGTGG ACTCAATAAA 
CCAGGCACAG AAAACACCGA CGTCCAGAAG CCGCGCCGGA GCCTTGAAGT CATACCCGAA 
AAAGCAAACG ATGAAACTGG AGAATGA