LOC101361801 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101361801
Entrez Gene ID	101361801
Full Name	DNA polymerase nu
Synonyms	POLN
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004383565.1	XP_004383622.1	DNA polymerase nu
XM_023737981.1	XP_023593749.1	DNA polymerase nu

Pathways from BioSystems
Gene Ontology

The following LOC101361801 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101361801 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr181160	XM_004383565.1 Latest version!	Trichechus manatus latirostris polymerase (DNA directed) nu (LOC101361801), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTr223651	XM_023737981.1 Latest version!	Trichechus manatus latirostris DNA polymerase nu (LOC101361801), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101361801 ( XM_004383565.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004383622.1 Trichechus manatus latirostris polymerase (DNA directed) nu (LOC101361801), mRNA.

Price & Availability↑

CloneID	OTr181160
Clone ID Related Accession (Same CDS sequence)	XM_004383565.1
Accession Version	XM_004383565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	DNA polymerase nu
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGAAAATT CTGAGGCATT TGTAGGCTTT GATCTCTATA AGACACCGCT CTCCAGCATT 
GCTCAGAAGA TTATGTCTGC TATGCATTCA GTTGATTTAG TGGATTCTGA GAATTTGAGA 
GAGAGTGAAA AGACTGCAGA AGTGGTATGC AAATCCAGTG TTAAGTCTTC AGTACTACAT 
GAAGATGGGA AGAATCAATC ACTGGGAAGA AAGAATCTTA TTTCTTTAAC AAGCCAGACA 
CCAAGAACTT CCATCAAGTT CCATCCTCAG TCGGCCAGTG TCAGCCTTAC AAATCAGCTG 
TCTCCTGACC AAAACCAGGA AAGCATCAGC TCCCTGGCTC TTTCCAATTG TTTAATTCCA 
CGGAGTCATC AAGAGGCTTC AGTTCTACAG AAAATGGAGT GTAAAAGAAA GCACTTCCTA 
AAAGAAAATG TCAGTGAAAA TGGAAATAAG GAAAGCATGA GTCTTAAAAG AAAACACATT 
GCATCTAATA ATTTATTAGA GAAAACAAGT AAACATAGGA CATTGGAAGA AGATGAGAAG 
GAGGTTCAAG CCTACGTAAA TTCTGGGAAC TCAAGTGCAT TTAAAAACCA TTTTTGTGAT 
ACTAGGTATT TGGGAGATTT GGAGAAAAGC CAGCTGACTG AAATGCTCAA ACAGGCAGCA 
GCCCTGGTGG TAACTCTGAT GTATAAGGAT GGTTCAACCC AGCTAAGAAC TGACCAGGCT 
ACCGTTTCTT CTGTTAAAGG AATTGTAATA TTACTAAAGA GCCAAGTGGA AGAAGGCAGT 
GGTTCTCTGG CTGCCTCAGC CTCTGATGGC GTTCTTGAGG GGTTCATTTC TAATGATAGG 
TATATTTACA TAAAAATAGA GCACTCTTCT ATTTGGGGCC AGGAACAAGA GATACACACT 
CAGTTTGCCA GGAAAGTGCT ATTTCAAGCA CTGAAATGTA AGTGTCCTGT TATTTGTTTT 
AATGGGAAGG ATTTTGTGAG AGCTGTGCTC CAGTTTCTTG GTGACGATGG CAGCTGGAAG 
CAGGTTGCTG ATTTTGTAGG GTTAGATCCC AGAATTGCTG CATGGCTTAT AGAGCCCAGT 
GACACTGAGC CTTCTTTTGA AGATTTAGTG GCAAAATATT TTGAAAAATC CATCACAGTT 
AAAGTAAACA GCACATATGG AAATTCGTCA AGAAATATTG TGAATCAGAA TGTGTGTGTG 
AACCTGAGGA TACTCTACAG ACTTATGATG GAACTGTGTT CTAAACTGAA GGGTTATGGT 
TTATGGCAAC TATTTTGTAC TTTGGAGCTT CCTCTGATAC CAATTTTGGC AGTGATGGAA 
AGCAACAGCA TTCAAATTAA CAAGGAAGAA ATGAGGAGGA CTTCAGCTCT GCTTGGGTCT 
CGTCTCAAGG AATTGGAACA AGAAGCTCAT TTTGTTGCAG GAGAACAGTT TCTTATAACA 
AGTAATCATC AGCTCCGGGA GATTCTATTT GGCAAATTAA AGCTGCACCT GCTGTGTCAA 
AACAAGACGC TTCCCACAAC TGCACTGCAG AAACACCCGT CCACCTCCGA GGCCGTGTTG 
AGTGTTCTGC AAGACCTTCA TCCATTACCC AAGATCATTT TGGAATATAG GCAGGTTCAC 
AAGATCAAGT CAACCTTTGT AGATGGATTG CTAGCTTGCA TGAAAAAGGG CTTCATTTCC 
TCTACATGGA ACCAAACTGG AACTGTGACT GGAAGACTTT CAGCCAAGCA TCCTAATATC 
CAAGGTATCT CCAAGCATCC AATTCAAATT ACTAAACCTC AGAATTTTAA AGGGAAGGAA 
GATGAGGCCA TCACCATCTC CCCCAGGACC ATGTTTGTTT CATCCAAAGG CCACACCTTC 
CTGGCAGCAG ACTTTTCACA GACTGAGTTG CGCATTCTTG CACACTTATC TGGGGATCCG 
GAACTTTTGA AGTTGTTCCA GGAATCTGAG AGAGATGATG TCTTTTTGAC CCTGACTTCA 
CAGTGGAAGG CCATTCCCGT GGAGCATGTG AAACATGTGG ACAGAGAGCA GACCAAGAAG 
GTGGTGTACT CAGTGATCTA TGGAGCAGGG AAGGAGCGGC TTGCTGCTTG CCTTGGAGTT 
ACTGTTCAGG AAGCTGCCCA GTTTTTGGAG AGCTTTTTGC AGAAATACAA GAAAATCAAG 
GATTTTGCCC AAGCAACTAT TGCCCAGTGT CACCAGACAG GGTACGTGGC GTCCATCATG 
GGCAGAAGGA GACCCCTGCC AAGGATCCAC GCACGGGACC AGAAGCTTCG GGCACAAGCA 
GAGCGGCAGG CAGTGAACTT CGTGGTTCAA GGCTCGGCTG CTGACCTCTG CAAGCTGGCC 
ATGATCCACA TCTTCACCGC AGTGGCCTCT TCTCCCACCC TGACGGCCAG GTTGGTTGCC 
CAAATTCACG ACGAGCTGCT GTTTGAGGTG GAAGACCCAC AGGTTCCCGA GTTTGCAGCT 
CTCGTCAGGG CCACCATGGA GTCCTTGCAG CACATCCGGG CCCTGGGGCT GCAGCTGCAG 
GTGCCCCTGC GGGTGAGCTT GAGTGCCGGC CGCAAGTGGG GCTGCCTGGC CCCGCTGCAG 
GAGACCCTGG GCCCAGCCCC ACGGCTCCCC CCGCCCCACA CCGAGTCTCC AAGCAACAGC 
CCGGCCACCT CTGCCAGATG CCCGGGCAGC ACCTTCCACC GAGTTGCCAG CCACCTGGTT 
GGTCCAGCAG TGTCCTCCCT GCACACCGCC TCAGCCTCCC GTATCCACGG CCCCATCGCT 
CCGGCAGCGC GGCCCCACAG GCAGGCCCTG CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004383622.1
CDS	1..2796
Translation	MENSEAFVGFDLYKTPLSSIAQKIMSAMHSVDLVDSENLRESEKTAEVVCKSSVKSSVLHEDGKNQSLGRKNLISLTSQTPRTSIKFHPQSASVSLTNQLSPDQNQESISSLALSNCLIPRSHQEASVLQKMECKRKHFLKENVSENGNKESMSLKRKHIASNNLLEKTSKHRTLEEDEKEVQAYVNSGNSSAFKNHFCDTRYLGDLEKSQLTEMLKQAAALVVTLMYKDGSTQLRTDQATVSSVKGIVILLKSQVEEGSGSLAASASDGVLEGFISNDRYIYIKIEHSSIWGQEQEIHTQFARKVLFQALKCKCPVICFNGKDFVRAVLQFLGDDGSWKQVADFVGLDPRIAAWLIEPSDTEPSFEDLVAKYFEKSITVKVNSTYGNSSRNIVNQNVCVNLRILYRLMMELCSKLKGYGLWQLFCTLELPLIPILAVMESNSIQINKEEMRRTSALLGSRLKELEQEAHFVAGEQFLITSNHQLREILFGKLKLHLLCQNKTLPTTALQKHPSTSEAVLSVLQDLHPLPKIILEYRQVHKIKSTFVDGLLACMKKGFISSTWNQTGTVTGRLSAKHPNIQGISKHPIQITKPQNFKGKEDEAITISPRTMFVSSKGHTFLAADFSQTELRILAHLSGDPELLKLFQESERDDVFLTLTSQWKAIPVEHVKHVDREQTKKVVYSVIYGAGKERLAACLGVTVQEAAQFLESFLQKYKKIKDFAQATIAQCHQTGYVASIMGRRRPLPRIHARDQKLRAQAERQAVNFVVQGSAADLCKLAMIHIFTAVASSPTLTARLVAQIHDELLFEVEDPQVPEFAALVRATMESLQHIRALGLQLQVPLRVSLSAGRKWGCLAPLQETLGPAPRLPPPHTESPSNSPATSARCPGSTFHRVASHLVGPAVSSLHTASASRIHGPIAPAARPHRQALR

Target ORF information:

RefSeq Version	XM_004383565.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris polymerase (DNA directed) nu (LOC101361801), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004383565.1

ORF Insert Sequence:

ATGGAAAATT CTGAGGCATT TGTAGGCTTT GATCTCTATA AGACACCGCT CTCCAGCATT 
GCTCAGAAGA TTATGTCTGC TATGCATTCA GTTGATTTAG TGGATTCTGA GAATTTGAGA 
GAGAGTGAAA AGACTGCAGA AGTGGTATGC AAATCCAGTG TTAAGTCTTC AGTACTACAT 
GAAGATGGGA AGAATCAATC ACTGGGAAGA AAGAATCTTA TTTCTTTAAC AAGCCAGACA 
CCAAGAACTT CCATCAAGTT CCATCCTCAG TCGGCCAGTG TCAGCCTTAC AAATCAGCTG 
TCTCCTGACC AAAACCAGGA AAGCATCAGC TCCCTGGCTC TTTCCAATTG TTTAATTCCA 
CGGAGTCATC AAGAGGCTTC AGTTCTACAG AAAATGGAGT GTAAAAGAAA GCACTTCCTA 
AAAGAAAATG TCAGTGAAAA TGGAAATAAG GAAAGCATGA GTCTTAAAAG AAAACACATT 
GCATCTAATA ATTTATTAGA GAAAACAAGT AAACATAGGA CATTGGAAGA AGATGAGAAG 
GAGGTTCAAG CCTACGTAAA TTCTGGGAAC TCAAGTGCAT TTAAAAACCA TTTTTGTGAT 
ACTAGGTATT TGGGAGATTT GGAGAAAAGC CAGCTGACTG AAATGCTCAA ACAGGCAGCA 
GCCCTGGTGG TAACTCTGAT GTATAAGGAT GGTTCAACCC AGCTAAGAAC TGACCAGGCT 
ACCGTTTCTT CTGTTAAAGG AATTGTAATA TTACTAAAGA GCCAAGTGGA AGAAGGCAGT 
GGTTCTCTGG CTGCCTCAGC CTCTGATGGC GTTCTTGAGG GGTTCATTTC TAATGATAGG 
TATATTTACA TAAAAATAGA GCACTCTTCT ATTTGGGGCC AGGAACAAGA GATACACACT 
CAGTTTGCCA GGAAAGTGCT ATTTCAAGCA CTGAAATGTA AGTGTCCTGT TATTTGTTTT 
AATGGGAAGG ATTTTGTGAG AGCTGTGCTC CAGTTTCTTG GTGACGATGG CAGCTGGAAG 
CAGGTTGCTG ATTTTGTAGG GTTAGATCCC AGAATTGCTG CATGGCTTAT AGAGCCCAGT 
GACACTGAGC CTTCTTTTGA AGATTTAGTG GCAAAATATT TTGAAAAATC CATCACAGTT 
AAAGTAAACA GCACATATGG AAATTCGTCA AGAAATATTG TGAATCAGAA TGTGTGTGTG 
AACCTGAGGA TACTCTACAG ACTTATGATG GAACTGTGTT CTAAACTGAA GGGTTATGGT 
TTATGGCAAC TATTTTGTAC TTTGGAGCTT CCTCTGATAC CAATTTTGGC AGTGATGGAA 
AGCAACAGCA TTCAAATTAA CAAGGAAGAA ATGAGGAGGA CTTCAGCTCT GCTTGGGTCT 
CGTCTCAAGG AATTGGAACA AGAAGCTCAT TTTGTTGCAG GAGAACAGTT TCTTATAACA 
AGTAATCATC AGCTCCGGGA GATTCTATTT GGCAAATTAA AGCTGCACCT GCTGTGTCAA 
AACAAGACGC TTCCCACAAC TGCACTGCAG AAACACCCGT CCACCTCCGA GGCCGTGTTG 
AGTGTTCTGC AAGACCTTCA TCCATTACCC AAGATCATTT TGGAATATAG GCAGGTTCAC 
AAGATCAAGT CAACCTTTGT AGATGGATTG CTAGCTTGCA TGAAAAAGGG CTTCATTTCC 
TCTACATGGA ACCAAACTGG AACTGTGACT GGAAGACTTT CAGCCAAGCA TCCTAATATC 
CAAGGTATCT CCAAGCATCC AATTCAAATT ACTAAACCTC AGAATTTTAA AGGGAAGGAA 
GATGAGGCCA TCACCATCTC CCCCAGGACC ATGTTTGTTT CATCCAAAGG CCACACCTTC 
CTGGCAGCAG ACTTTTCACA GACTGAGTTG CGCATTCTTG CACACTTATC TGGGGATCCG 
GAACTTTTGA AGTTGTTCCA GGAATCTGAG AGAGATGATG TCTTTTTGAC CCTGACTTCA 
CAGTGGAAGG CCATTCCCGT GGAGCATGTG AAACATGTGG ACAGAGAGCA GACCAAGAAG 
GTGGTGTACT CAGTGATCTA TGGAGCAGGG AAGGAGCGGC TTGCTGCTTG CCTTGGAGTT 
ACTGTTCAGG AAGCTGCCCA GTTTTTGGAG AGCTTTTTGC AGAAATACAA GAAAATCAAG 
GATTTTGCCC AAGCAACTAT TGCCCAGTGT CACCAGACAG GGTACGTGGC GTCCATCATG 
GGCAGAAGGA GACCCCTGCC AAGGATCCAC GCACGGGACC AGAAGCTTCG GGCACAAGCA 
GAGCGGCAGG CAGTGAACTT CGTGGTTCAA GGCTCGGCTG CTGACCTCTG CAAGCTGGCC 
ATGATCCACA TCTTCACCGC AGTGGCCTCT TCTCCCACCC TGACGGCCAG GTTGGTTGCC 
CAAATTCACG ACGAGCTGCT GTTTGAGGTG GAAGACCCAC AGGTTCCCGA GTTTGCAGCT 
CTCGTCAGGG CCACCATGGA GTCCTTGCAG CACATCCGGG CCCTGGGGCT GCAGCTGCAG 
GTGCCCCTGC GGGTGAGCTT GAGTGCCGGC CGCAAGTGGG GCTGCCTGGC CCCGCTGCAG 
GAGACCCTGG GCCCAGCCCC ACGGCTCCCC CCGCCCCACA CCGAGTCTCC AAGCAACAGC 
CCGGCCACCT CTGCCAGATG CCCGGGCAGC ACCTTCCACC GAGTTGCCAG CCACCTGGTT 
GGTCCAGCAG TGTCCTCCCT GCACACCGCC TCAGCCTCCC GTATCCACGG CCCCATCGCT 
CCGGCAGCGC GGCCCCACAG GCAGGCCCTG CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101361801 ( XM_023737981.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023593749.1 Trichechus manatus latirostris DNA polymerase nu (LOC101361801), mRNA.

Price & Availability↑

CloneID	OTr223651
Clone ID Related Accession (Same CDS sequence)	XM_023737981.1
Accession Version	XM_023737981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	DNA polymerase nu
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG AAAATTCTGA GGCATTTGTA GGCTTTGATC TCTATAAGAC ACCGCTCTCC 
AGCATTGCTC AGAAGATTAT GTCTGCTATG CATTCAGTTG ATTTAGTGGA TTCTGAGAAT 
TTGAGAGAGA GTGAAAAGAC TGCAGAAGTG GTATGCAAAT CCAGTGTTAA GTCTTCAGTA 
CTACATGAAG ATGGGAAGAA TCAATCACTG GGAAGAAAGA ATCTTATTTC TTTAACAAGC 
CAGACACCAA GAACTTCCAT CAAGTTCCAT CCTCAGTCGG CCAGTGTCAG CCTTACAAAT 
CAGCTGTCTC CTGACCAAAA CCAGGAAAGC ATCAGCTCCC TGGCTCTTTC CAATTGTTTA 
ATTCCACGGA GTCATCAAGA GGCTTCAGTT CTACAGAAAA TGGAGTGTAA AAGAAAGCAC 
TTCCTAAAAG AAAATGTCAG TGAAAATGGA AATAAGGAAA GCATGAGTCT TAAAAGAAAA 
CACATTGCAT CTAATAATTT ATTAGAGAAA ACAAGTAAAC ATAGGACATT GGAAGAAGAT 
GAGAAGGAGG TTCAAGCCTA CGTAAATTCT GGGAACTCAA GTGCATTTAA AAACCATTTT 
TGTGATACTA GGTATTTGGG AGATTTGGAG AAAAGCCAGC TGACTGAAAT GCTCAAACAG 
GCAGCAGCCC TGGTGGTAAC TCTGATGTAT AAGGATGGTT CAACCCAGCT AAGAACTGAC 
CAGGCTACCG TTTCTTCTGT TAAAGGAATT GTAATATTAC TAAAGAGCCA AGTGGAAGAA 
GGCAGTGGTT CTCTGGCTGC CTCAGCCTCT GATGGCGTTC TTGAGGGGTT CATTTCTAAT 
GATAGGTATA TTTACATAAA AATAGAGCAC TCTTCTATTT GGGGCCAGGA ACAAGAGATA 
CACACTCAGT TTGCCAGGAA AGTGCTATTT CAAGCACTGA AATGTAAGTG TCCTGTTATT 
TGTTTTAATG GGAAGGATTT TGTGAGAGCT GTGCTCCAGT TTCTTGGTGA CGATGGCAGC 
TGGAAGCAGG TTGCTGATTT TGTAGGGTTA GATCCCAGAA TTGCTGCATG GCTTATAGAG 
CCCAGTGACA CTGAGCCTTC TTTTGAAGAT TTAGTGGCAA AATATTTTGA AAAATCCATC 
ACAGTTAAAG TAAACAGCAC ATATGGAAAT TCGTCAAGAA ATATTGTGAA TCAGAATGTG 
TGTGTGAACC TGAGGATACT CTACAGACTT ATGATGGAAC TGTGTTCTAA ACTGAAGGGT 
TATGGTTTAT GGCAACTATT TTGTACTTTG GAGCTTCCTC TGATACCAAT TTTGGCAGTG 
ATGGAAAGCA ACAGCATTCA AATTAACAAG GAAGAAATGA GGAGGACTTC AGCTCTGCTT 
GGGTCTCGTC TCAAGGAATT GGAACAAGAA GCTCATTTTG TTGCAGGAGA ACAGTTTCTT 
ATAACAAGTA ATCATCAGCT CCGGGAGATT CTATTTGGCA AATTAAAGCT GCACCTGCTG 
TGTCAAAACA AGACGCTTCC CACAACTGCA CTGCAGAAAC ACCCGTCCAC CTCCGAGGCC 
GTGTTGAGTG TTCTGCAAGA CCTTCATCCA TTACCCAAGA TCATTTTGGA ATATAGGCAG 
GTTCACAAGA TCAAGTCAAC CTTTGTAGAT GGATTGCTAG CTTGCATGAA AAAGGGCTTC 
ATTTCCTCTA CATGGAACCA AACTGGAACT GTGACTGGAA GACTTTCAGC CAAGCATCCT 
AATATCCAAG GTATCTCCAA GCATCCAATT CAAATTACTA AACCTCAGAA TTTTAAAGGG 
AAGGAAGATG AGGCCATCAC CATCTCCCCC AGGACCATGT TTGTTTCATC CAAAGGCCAC 
ACCTTCCTGG CAGCAGACTT TTCACAGACT GAGTTGCGCA TTCTTGCACA CTTATCTGGG 
GATCCGGAAC TTTTGAAGTT GTTCCAGGAA TCTGAGAGAG ATGATGTCTT TTTGACCCTG 
ACTTCACAGT GGAAGGCCAT TCCCGTGGAG CATGTGAAAC ATGTGGACAG AGAGCAGACC 
AAGAAGGTGG TGTACTCAGT GATCTATGGA GCAGGGAAGG AGCGGCTTGC TGCTTGCCTT 
GGAGTTACTG TTCAGGAAGC TGCCCAGTTT TTGGAGAGCT TTTTGCAGAA ATACAAGAAA 
ATCAAGGATT TTGCCCAAGC AACTATTGCC CAGTGTCACC AGACAGGGTA CGTGGCGTCC 
ATCATGGGCA GAAGGAGACC CCTGCCAAGG ATCCACGCAC GGGACCAGAA GCTTCGGGCA 
CAAGCAGAGC GGCAGGCAGT GAACTTCGTG GTTCAAGGCT CGGCTGCTGA CCTCTGCAAG 
CTGGCCATGA TCCACATCTT CACCGCAGTG GCCTCTTCTC CCACCCTGAC GGCCAGGTTG 
GTTGCCCAAA TTCACGACGA GCTGCTGTTT GAGGTGGAAG ACCCACAGGT TCCCGAGTTT 
GCAGCTCTCG TCAGGGCCAC CATGGAGTCC TTGCAGCACA TCCGGGCCCT GGGGCTGCAG 
CTGCAGGACC CTCCACCCTT GGCCACCTGG CCTCTGGCCG ACACCGCCTT CTCTGAGGCT 
CACCCCTTCG TGGGGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023593749.1
CDS	1766..4366
Translation	MKMENSEAFVGFDLYKTPLSSIAQKIMSAMHSVDLVDSENLRESEKTAEVVCKSSVKSSVLHEDGKNQSLGRKNLISLTSQTPRTSIKFHPQSASVSLTNQLSPDQNQESISSLALSNCLIPRSHQEASVLQKMECKRKHFLKENVSENGNKESMSLKRKHIASNNLLEKTSKHRTLEEDEKEVQAYVNSGNSSAFKNHFCDTRYLGDLEKSQLTEMLKQAAALVVTLMYKDGSTQLRTDQATVSSVKGIVILLKSQVEEGSGSLAASASDGVLEGFISNDRYIYIKIEHSSIWGQEQEIHTQFARKVLFQALKCKCPVICFNGKDFVRAVLQFLGDDGSWKQVADFVGLDPRIAAWLIEPSDTEPSFEDLVAKYFEKSITVKVNSTYGNSSRNIVNQNVCVNLRILYRLMMELCSKLKGYGLWQLFCTLELPLIPILAVMESNSIQINKEEMRRTSALLGSRLKELEQEAHFVAGEQFLITSNHQLREILFGKLKLHLLCQNKTLPTTALQKHPSTSEAVLSVLQDLHPLPKIILEYRQVHKIKSTFVDGLLACMKKGFISSTWNQTGTVTGRLSAKHPNIQGISKHPIQITKPQNFKGKEDEAITISPRTMFVSSKGHTFLAADFSQTELRILAHLSGDPELLKLFQESERDDVFLTLTSQWKAIPVEHVKHVDREQTKKVVYSVIYGAGKERLAACLGVTVQEAAQFLESFLQKYKKIKDFAQATIAQCHQTGYVASIMGRRRPLPRIHARDQKLRAQAERQAVNFVVQGSAADLCKLAMIHIFTAVASSPTLTARLVAQIHDELLFEVEDPQVPEFAALVRATMESLQHIRALGLQLQDPPPLATWPLADTAFSEAHPFVGL

Target ORF information:

RefSeq Version	XM_023737981.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris DNA polymerase nu (LOC101361801), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023737981.1

ORF Insert Sequence:

ATGAAAATGG AAAATTCTGA GGCATTTGTA GGCTTTGATC TCTATAAGAC ACCGCTCTCC 
AGCATTGCTC AGAAGATTAT GTCTGCTATG CATTCAGTTG ATTTAGTGGA TTCTGAGAAT 
TTGAGAGAGA GTGAAAAGAC TGCAGAAGTG GTATGCAAAT CCAGTGTTAA GTCTTCAGTA 
CTACATGAAG ATGGGAAGAA TCAATCACTG GGAAGAAAGA ATCTTATTTC TTTAACAAGC 
CAGACACCAA GAACTTCCAT CAAGTTCCAT CCTCAGTCGG CCAGTGTCAG CCTTACAAAT 
CAGCTGTCTC CTGACCAAAA CCAGGAAAGC ATCAGCTCCC TGGCTCTTTC CAATTGTTTA 
ATTCCACGGA GTCATCAAGA GGCTTCAGTT CTACAGAAAA TGGAGTGTAA AAGAAAGCAC 
TTCCTAAAAG AAAATGTCAG TGAAAATGGA AATAAGGAAA GCATGAGTCT TAAAAGAAAA 
CACATTGCAT CTAATAATTT ATTAGAGAAA ACAAGTAAAC ATAGGACATT GGAAGAAGAT 
GAGAAGGAGG TTCAAGCCTA CGTAAATTCT GGGAACTCAA GTGCATTTAA AAACCATTTT 
TGTGATACTA GGTATTTGGG AGATTTGGAG AAAAGCCAGC TGACTGAAAT GCTCAAACAG 
GCAGCAGCCC TGGTGGTAAC TCTGATGTAT AAGGATGGTT CAACCCAGCT AAGAACTGAC 
CAGGCTACCG TTTCTTCTGT TAAAGGAATT GTAATATTAC TAAAGAGCCA AGTGGAAGAA 
GGCAGTGGTT CTCTGGCTGC CTCAGCCTCT GATGGCGTTC TTGAGGGGTT CATTTCTAAT 
GATAGGTATA TTTACATAAA AATAGAGCAC TCTTCTATTT GGGGCCAGGA ACAAGAGATA 
CACACTCAGT TTGCCAGGAA AGTGCTATTT CAAGCACTGA AATGTAAGTG TCCTGTTATT 
TGTTTTAATG GGAAGGATTT TGTGAGAGCT GTGCTCCAGT TTCTTGGTGA CGATGGCAGC 
TGGAAGCAGG TTGCTGATTT TGTAGGGTTA GATCCCAGAA TTGCTGCATG GCTTATAGAG 
CCCAGTGACA CTGAGCCTTC TTTTGAAGAT TTAGTGGCAA AATATTTTGA AAAATCCATC 
ACAGTTAAAG TAAACAGCAC ATATGGAAAT TCGTCAAGAA ATATTGTGAA TCAGAATGTG 
TGTGTGAACC TGAGGATACT CTACAGACTT ATGATGGAAC TGTGTTCTAA ACTGAAGGGT 
TATGGTTTAT GGCAACTATT TTGTACTTTG GAGCTTCCTC TGATACCAAT TTTGGCAGTG 
ATGGAAAGCA ACAGCATTCA AATTAACAAG GAAGAAATGA GGAGGACTTC AGCTCTGCTT 
GGGTCTCGTC TCAAGGAATT GGAACAAGAA GCTCATTTTG TTGCAGGAGA ACAGTTTCTT 
ATAACAAGTA ATCATCAGCT CCGGGAGATT CTATTTGGCA AATTAAAGCT GCACCTGCTG 
TGTCAAAACA AGACGCTTCC CACAACTGCA CTGCAGAAAC ACCCGTCCAC CTCCGAGGCC 
GTGTTGAGTG TTCTGCAAGA CCTTCATCCA TTACCCAAGA TCATTTTGGA ATATAGGCAG 
GTTCACAAGA TCAAGTCAAC CTTTGTAGAT GGATTGCTAG CTTGCATGAA AAAGGGCTTC 
ATTTCCTCTA CATGGAACCA AACTGGAACT GTGACTGGAA GACTTTCAGC CAAGCATCCT 
AATATCCAAG GTATCTCCAA GCATCCAATT CAAATTACTA AACCTCAGAA TTTTAAAGGG 
AAGGAAGATG AGGCCATCAC CATCTCCCCC AGGACCATGT TTGTTTCATC CAAAGGCCAC 
ACCTTCCTGG CAGCAGACTT TTCACAGACT GAGTTGCGCA TTCTTGCACA CTTATCTGGG 
GATCCGGAAC TTTTGAAGTT GTTCCAGGAA TCTGAGAGAG ATGATGTCTT TTTGACCCTG 
ACTTCACAGT GGAAGGCCAT TCCCGTGGAG CATGTGAAAC ATGTGGACAG AGAGCAGACC 
AAGAAGGTGG TGTACTCAGT GATCTATGGA GCAGGGAAGG AGCGGCTTGC TGCTTGCCTT 
GGAGTTACTG TTCAGGAAGC TGCCCAGTTT TTGGAGAGCT TTTTGCAGAA ATACAAGAAA 
ATCAAGGATT TTGCCCAAGC AACTATTGCC CAGTGTCACC AGACAGGGTA CGTGGCGTCC 
ATCATGGGCA GAAGGAGACC CCTGCCAAGG ATCCACGCAC GGGACCAGAA GCTTCGGGCA 
CAAGCAGAGC GGCAGGCAGT GAACTTCGTG GTTCAAGGCT CGGCTGCTGA CCTCTGCAAG 
CTGGCCATGA TCCACATCTT CACCGCAGTG GCCTCTTCTC CCACCCTGAC GGCCAGGTTG 
GTTGCCCAAA TTCACGACGA GCTGCTGTTT GAGGTGGAAG ACCCACAGGT TCCCGAGTTT 
GCAGCTCTCG TCAGGGCCAC CATGGAGTCC TTGCAGCACA TCCGGGCCCT GGGGCTGCAG 
CTGCAGGACC CTCCACCCTT GGCCACCTGG CCTCTGGCCG ACACCGCCTT CTCTGAGGCT 
CACCCCTTCG TGGGGCTGTA G