LOC101360776 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101360776
Entrez Gene ID	101360776
Full Name	cell cycle and apoptosis regulator 2
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004382123.1	XP_004382180.1	cell cycle and apoptosis regulator protein 2
XM_004382123.2	XP_004382180.1	cell cycle and apoptosis regulator protein 2

Pathways from BioSystems
Gene Ontology

The following LOC101360776 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101360776 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr179613	XM_004382123.2 Latest version!	Trichechus manatus latirostris cell cycle and apoptosis regulator 2 (LOC101360776), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OTr179613	XM_004382123.1	Trichechus manatus latirostris cell cycle and apoptosis regulator 2 (LOC101360776), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101360776 ( XM_004382123.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004382180.1 Trichechus manatus latirostris cell cycle and apoptosis regulator 2 (LOC101360776), mRNA.

Price & Availability↑

CloneID	OTr179613
Clone ID Related Accession (Same CDS sequence)	XM_004382123.1 , XM_004382123.2
Accession Version	XM_004382123.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	cell cycle and apoptosis regulator protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444026.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGT TTAAGCGCCA GCGGATCAAT CCGCTTCCAG GGGGACGCAA CTTTTCAGGC 
GCAGCTTCAA CATCTCTTCT GGGACCTCCT CCTGGTTTGC TCACTCCTCC TGTGGCTACA 
GATCTGTCCC AAAATGCCAG GCACCTTCAG GGTGGGGAGA AGCAGCGGGT CTTCACTGGA 
ATTGTTACCA GTTTGCACGA CTACTTTGGG GTGGTGGATG AAGAGGTTTT TTTTCAGCTA 
AGTGTGGTGA AGGGGCGGCT GCCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCATAC 
AACCCAGGCC AGGCAGTGCC TTGGAATGCT GTCAAGGTGC AAACACTCTC CAACCAGCCC 
CTGCTGAAGT CGCCAGCACC TCCCCTTCTG CACGTGGCAG CCCTGGGCCA GAAGCAAGGC 
ATCCTGGGAG CTCAGCCTCA GTTGATCTTC CAGCATCACC GAATTCCCCC TCTCTTTCCT 
CAGAAGCCTC TGAGTCTCTT CCAAACATCC CACACTCTTC ACCTGAGCCA CTTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGATTG GATCAGGGCC GAAGTGATGA CTATGACTCC 
AAGAAACGTA AACAACGGGC TGGCGGAGAG CCTTGGGGTG CCAAGAAACC AAGGCATGAC 
CTGCCTCCTT ACCGGGTCCA CCTCACTCCC TATACTGTGG ACAGCCCCAT CTGTGACTTC 
TTAGAACTCC AGCGCCGTTA CCGCAGCCTC CTGGTTCCCT CAGATTTTCT GGCTGTTCAT 
CTGAGCTGGC TGTCAGCCTT CCCTCTGAGC CAGCCCTTTT CCCTCCACCA TCCAAGCCGT 
ATCCAGGTAT CTTCTGAGAA GGAGCCAGCT CCAGACACTG GTGCTGAGCC TATCCCTTCA 
GACAGTGACC CCGGATATAG TTCCAAGGTA CTGTTGCTCT CTTCCCCTGG ATTGGAGGAA 
TTGTATCGTT GTTGTATGCT GTTTGTGGAT GACATCGCTG AGCCGAGGGA GACGCCAGAA 
CATCCTTTGA AGCAGATTAA GTTTTTGCTG GGCCGGAAAG ATGAAGAGGC AGTGCTGGTT 
GGGGGCGAGT GGTCTCCTTC CTTGGATGGC CTTGACCCCA AGGCTGACCC GCAGGTGCTA 
GTCCGCACCG CGATCCGGTG TGCACAGGTT CAGACTGGCA TTGACTTGAG CAGCTGCACA 
AAGTGGTGGC GCTTTGCTGA GTTTCAGTAC CTGCAGCCGG GACCCCCAAG GCGGCTCCAG 
ACAGTGGTGG TGTACCTGCC GGATGTCTGG ACCATTATGC CTACTTTGGA AGAGTGGGAG 
GCCCTGTGCC AGCAGAAAGC TGCAGAGGCA GCTCTCCCAC CGCAGGAGTC ACCAGTGGAA 
ACAGAGTCTT CTGAACAGGC GGCAGATGCA TCGGAGCAAG CAGCAGACAC CTCTAAACAA 
AATGCAGAGA ATCCGGAGGT CACCGCGCAG CAGGAAATGG ACACAGATCT CCCAGAGGCC 
CCTCCACCTC CTCTAGAACC TGCTGTCATT GCACGCTCTG ACTGTATAAA CCTCTCTCTC 
CATGGTATTG TGGAGGATCG GAGGCCAAAA GAAAGAATCT CTTTTGAGGT GATGGTGTTG 
GCTGAGCTAT TTCTGGAGAT GCTGCAGAGG GATTTTGGCT ATAGGATTTA TAAGATGCTG 
CTGAGCCTTC CTGAAAAGGT AGTGACTCCA CCTGAACCTG AGAAGGAGGA GCCAGCCAAG 
GAGGAAGCAG TTAAGGAGGA AGAAGTGGTC AAAGAGGAGG TGGCCAAGGA GTCGAAGGAT 
GAGGTACAGA ATGAGGGAGC AGCTGCTGAG TCAGACACCC CACTGAAGGA GGATGGACTT 
TTGCCCAAAC CCCCATCTTC TGGGGGAGAG GAAGAAGAGA AGCCCCGGGG TGAGGCATCT 
GAGGACCTTT GTGAGATGGC TCTGGACCCA GAACTGCTGC TTCTGAGGGA TGATGGAGAA 
GAGGAATTTG CAGGAGCAAA GCTGGAGGAT TCGGAGGTAC GGTCCGTTGC CTCAAACCAG 
TCAGAGATGG AATTCTCTTC ACTTCAGGAC ATGCCCAAGG AGCTGGATCC CTCTGCTGTG 
CTCCCCTTGG ATTGTCTCCT TGCTTTTGTG TTCTTTGATG CCAACTGGTG TGGCTACTTG 
CACCGGAGAG ACTTGGAGAG GATTCTACTT ACCCTTGGGC TACGGCTTAG TGCAGAGCAG 
GCCAAGCAGC TGGTCAGCAG GGTGGTGACC CAGAACATCT GCCAGTACCG GAGCCTTCAG 
TATAGCCGCC AGGAGGGCAT GGATGGCGGA CTTCCTGAGG AGGTGCTCTG TGGAAACCTT 
GATCTGCTAC CTCCTCCTGG GAAAAGCGCA AAGCCAAGTG CTGCCCCCAT GGAACACAAG 
GGCCTGGTGT CCCACAATGG CAGCCTAATC AATGTGGGGA ACCTGCTGCA GCGTGCAGAG 
CAGCAGGACA GTGGACGGCT CTACCTGGAG AACAAGATTC ACACACTGGA ACTGAAGCTG 
GAGGAGAGCC ATAACCGATT CTCAGCCACT GAAGTGACAA ATAAGACACT GGCGGCAGAA 
ATGCAGGAGT TGCGAAATCG GTTGGCTGAG GCTGAGGAGA CAGCCCGGAC AGCTGAACGA 
CAGAAGAACC AGCTTCAGCG GCTGCTACAG GACTTCCGAA GGCGCCTGAC CCCTCTGCAG 
CTTGAGATGC AGCGAATGGT TGAAAAGGCC GACAGCTGGG TAGAGAAGGA GGAGCCAGCA 
CCTACCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004382180.1
CDS	248..3019
Translation	MSQFKRQRINPLPGGRNFSGAASTSLLGPPPGLLTPPVATDLSQNARHLQGGEKQRVFTGIVTSLHDYFGVVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQGILGAQPQLIFQHHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYTVDSPICDFLELQRRYRSLLVPSDFLAVHLSWLSAFPLSQPFSLHHPSRIQVSSEKEPAPDTGAEPIPSDSDPGYSSKVLLLSSPGLEELYRCCMLFVDDIAEPRETPEHPLKQIKFLLGRKDEEAVLVGGEWSPSLDGLDPKADPQVLVRTAIRCAQVQTGIDLSSCTKWWRFAEFQYLQPGPPRRLQTVVVYLPDVWTIMPTLEEWEALCQQKAAEAALPPQESPVETESSEQAADASEQAADTSKQNAENPEVTAQQEMDTDLPEAPPPPLEPAVIARSDCINLSLHGIVEDRRPKERISFEVMVLAELFLEMLQRDFGYRIYKMLLSLPEKVVTPPEPEKEEPAKEEAVKEEEVVKEEVAKESKDEVQNEGAAAESDTPLKEDGLLPKPPSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGLRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGMDGGLPEEVLCGNLDLLPPPGKSAKPSAAPMEHKGLVSHNGSLINVGNLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRNRLAEAEETARTAERQKNQLQRLLQDFRRRLTPLQLEMQRMVEKADSWVEKEEPAPTN

Target ORF information:

RefSeq Version	XM_004382123.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris cell cycle and apoptosis regulator 2 (LOC101360776), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004382123.1

ORF Insert Sequence:

ATGTCCCAGT TTAAGCGCCA GCGGATCAAT CCGCTTCCAG GGGGACGCAA CTTTTCAGGC 
GCAGCTTCAA CATCTCTTCT GGGACCTCCT CCTGGTTTGC TCACTCCTCC TGTGGCTACA 
GATCTGTCCC AAAATGCCAG GCACCTTCAG GGTGGGGAGA AGCAGCGGGT CTTCACTGGA 
ATTGTTACCA GTTTGCACGA CTACTTTGGG GTGGTGGATG AAGAGGTTTT TTTTCAGCTA 
AGTGTGGTGA AGGGGCGGCT GCCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCATAC 
AACCCAGGCC AGGCAGTGCC TTGGAATGCT GTCAAGGTGC AAACACTCTC CAACCAGCCC 
CTGCTGAAGT CGCCAGCACC TCCCCTTCTG CACGTGGCAG CCCTGGGCCA GAAGCAAGGC 
ATCCTGGGAG CTCAGCCTCA GTTGATCTTC CAGCATCACC GAATTCCCCC TCTCTTTCCT 
CAGAAGCCTC TGAGTCTCTT CCAAACATCC CACACTCTTC ACCTGAGCCA CTTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGATTG GATCAGGGCC GAAGTGATGA CTATGACTCC 
AAGAAACGTA AACAACGGGC TGGCGGAGAG CCTTGGGGTG CCAAGAAACC AAGGCATGAC 
CTGCCTCCTT ACCGGGTCCA CCTCACTCCC TATACTGTGG ACAGCCCCAT CTGTGACTTC 
TTAGAACTCC AGCGCCGTTA CCGCAGCCTC CTGGTTCCCT CAGATTTTCT GGCTGTTCAT 
CTGAGCTGGC TGTCAGCCTT CCCTCTGAGC CAGCCCTTTT CCCTCCACCA TCCAAGCCGT 
ATCCAGGTAT CTTCTGAGAA GGAGCCAGCT CCAGACACTG GTGCTGAGCC TATCCCTTCA 
GACAGTGACC CCGGATATAG TTCCAAGGTA CTGTTGCTCT CTTCCCCTGG ATTGGAGGAA 
TTGTATCGTT GTTGTATGCT GTTTGTGGAT GACATCGCTG AGCCGAGGGA GACGCCAGAA 
CATCCTTTGA AGCAGATTAA GTTTTTGCTG GGCCGGAAAG ATGAAGAGGC AGTGCTGGTT 
GGGGGCGAGT GGTCTCCTTC CTTGGATGGC CTTGACCCCA AGGCTGACCC GCAGGTGCTA 
GTCCGCACCG CGATCCGGTG TGCACAGGTT CAGACTGGCA TTGACTTGAG CAGCTGCACA 
AAGTGGTGGC GCTTTGCTGA GTTTCAGTAC CTGCAGCCGG GACCCCCAAG GCGGCTCCAG 
ACAGTGGTGG TGTACCTGCC GGATGTCTGG ACCATTATGC CTACTTTGGA AGAGTGGGAG 
GCCCTGTGCC AGCAGAAAGC TGCAGAGGCA GCTCTCCCAC CGCAGGAGTC ACCAGTGGAA 
ACAGAGTCTT CTGAACAGGC GGCAGATGCA TCGGAGCAAG CAGCAGACAC CTCTAAACAA 
AATGCAGAGA ATCCGGAGGT CACCGCGCAG CAGGAAATGG ACACAGATCT CCCAGAGGCC 
CCTCCACCTC CTCTAGAACC TGCTGTCATT GCACGCTCTG ACTGTATAAA CCTCTCTCTC 
CATGGTATTG TGGAGGATCG GAGGCCAAAA GAAAGAATCT CTTTTGAGGT GATGGTGTTG 
GCTGAGCTAT TTCTGGAGAT GCTGCAGAGG GATTTTGGCT ATAGGATTTA TAAGATGCTG 
CTGAGCCTTC CTGAAAAGGT AGTGACTCCA CCTGAACCTG AGAAGGAGGA GCCAGCCAAG 
GAGGAAGCAG TTAAGGAGGA AGAAGTGGTC AAAGAGGAGG TGGCCAAGGA GTCGAAGGAT 
GAGGTACAGA ATGAGGGAGC AGCTGCTGAG TCAGACACCC CACTGAAGGA GGATGGACTT 
TTGCCCAAAC CCCCATCTTC TGGGGGAGAG GAAGAAGAGA AGCCCCGGGG TGAGGCATCT 
GAGGACCTTT GTGAGATGGC TCTGGACCCA GAACTGCTGC TTCTGAGGGA TGATGGAGAA 
GAGGAATTTG CAGGAGCAAA GCTGGAGGAT TCGGAGGTAC GGTCCGTTGC CTCAAACCAG 
TCAGAGATGG AATTCTCTTC ACTTCAGGAC ATGCCCAAGG AGCTGGATCC CTCTGCTGTG 
CTCCCCTTGG ATTGTCTCCT TGCTTTTGTG TTCTTTGATG CCAACTGGTG TGGCTACTTG 
CACCGGAGAG ACTTGGAGAG GATTCTACTT ACCCTTGGGC TACGGCTTAG TGCAGAGCAG 
GCCAAGCAGC TGGTCAGCAG GGTGGTGACC CAGAACATCT GCCAGTACCG GAGCCTTCAG 
TATAGCCGCC AGGAGGGCAT GGATGGCGGA CTTCCTGAGG AGGTGCTCTG TGGAAACCTT 
GATCTGCTAC CTCCTCCTGG GAAAAGCGCA AAGCCAAGTG CTGCCCCCAT GGAACACAAG 
GGCCTGGTGT CCCACAATGG CAGCCTAATC AATGTGGGGA ACCTGCTGCA GCGTGCAGAG 
CAGCAGGACA GTGGACGGCT CTACCTGGAG AACAAGATTC ACACACTGGA ACTGAAGCTG 
GAGGAGAGCC ATAACCGATT CTCAGCCACT GAAGTGACAA ATAAGACACT GGCGGCAGAA 
ATGCAGGAGT TGCGAAATCG GTTGGCTGAG GCTGAGGAGA CAGCCCGGAC AGCTGAACGA 
CAGAAGAACC AGCTTCAGCG GCTGCTACAG GACTTCCGAA GGCGCCTGAC CCCTCTGCAG 
CTTGAGATGC AGCGAATGGT TGAAAAGGCC GACAGCTGGG TAGAGAAGGA GGAGCCAGCA 
CCTACCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101360776 ( XM_004382123.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004382180.1 Trichechus manatus latirostris cell cycle and apoptosis regulator 2 (LOC101360776), mRNA.

Price & Availability↑

CloneID	OTr179613
Clone ID Related Accession (Same CDS sequence)	XM_004382123.1 , XM_004382123.2
Accession Version	XM_004382123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	cell cycle and apoptosis regulator protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444026.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004382123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGT TTAAGCGCCA GCGGATCAAT CCGCTTCCAG GGGGACGCAA CTTTTCAGGC 
GCAGCTTCAA CATCTCTTCT GGGACCTCCT CCTGGTTTGC TCACTCCTCC TGTGGCTACA 
GATCTGTCCC AAAATGCCAG GCACCTTCAG GGTGGGGAGA AGCAGCGGGT CTTCACTGGA 
ATTGTTACCA GTTTGCACGA CTACTTTGGG GTGGTGGATG AAGAGGTTTT TTTTCAGCTA 
AGTGTGGTGA AGGGGCGGCT GCCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCATAC 
AACCCAGGCC AGGCAGTGCC TTGGAATGCT GTCAAGGTGC AAACACTCTC CAACCAGCCC 
CTGCTGAAGT CGCCAGCACC TCCCCTTCTG CACGTGGCAG CCCTGGGCCA GAAGCAAGGC 
ATCCTGGGAG CTCAGCCTCA GTTGATCTTC CAGCATCACC GAATTCCCCC TCTCTTTCCT 
CAGAAGCCTC TGAGTCTCTT CCAAACATCC CACACTCTTC ACCTGAGCCA CTTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGATTG GATCAGGGCC GAAGTGATGA CTATGACTCC 
AAGAAACGTA AACAACGGGC TGGCGGAGAG CCTTGGGGTG CCAAGAAACC AAGGCATGAC 
CTGCCTCCTT ACCGGGTCCA CCTCACTCCC TATACTGTGG ACAGCCCCAT CTGTGACTTC 
TTAGAACTCC AGCGCCGTTA CCGCAGCCTC CTGGTTCCCT CAGATTTTCT GGCTGTTCAT 
CTGAGCTGGC TGTCAGCCTT CCCTCTGAGC CAGCCCTTTT CCCTCCACCA TCCAAGCCGT 
ATCCAGGTAT CTTCTGAGAA GGAGCCAGCT CCAGACACTG GTGCTGAGCC TATCCCTTCA 
GACAGTGACC CCGGATATAG TTCCAAGGTA CTGTTGCTCT CTTCCCCTGG ATTGGAGGAA 
TTGTATCGTT GTTGTATGCT GTTTGTGGAT GACATCGCTG AGCCGAGGGA GACGCCAGAA 
CATCCTTTGA AGCAGATTAA GTTTTTGCTG GGCCGGAAAG ATGAAGAGGC AGTGCTGGTT 
GGGGGCGAGT GGTCTCCTTC CTTGGATGGC CTTGACCCCA AGGCTGACCC GCAGGTGCTA 
GTCCGCACCG CGATCCGGTG TGCACAGGTT CAGACTGGCA TTGACTTGAG CAGCTGCACA 
AAGTGGTGGC GCTTTGCTGA GTTTCAGTAC CTGCAGCCGG GACCCCCAAG GCGGCTCCAG 
ACAGTGGTGG TGTACCTGCC GGATGTCTGG ACCATTATGC CTACTTTGGA AGAGTGGGAG 
GCCCTGTGCC AGCAGAAAGC TGCAGAGGCA GCTCTCCCAC CGCAGGAGTC ACCAGTGGAA 
ACAGAGTCTT CTGAACAGGC GGCAGATGCA TCGGAGCAAG CAGCAGACAC CTCTAAACAA 
AATGCAGAGA ATCCGGAGGT CACCGCGCAG CAGGAAATGG ACACAGATCT CCCAGAGGCC 
CCTCCACCTC CTCTAGAACC TGCTGTCATT GCACGCTCTG ACTGTATAAA CCTCTCTCTC 
CATGGTATTG TGGAGGATCG GAGGCCAAAA GAAAGAATCT CTTTTGAGGT GATGGTGTTG 
GCTGAGCTAT TTCTGGAGAT GCTGCAGAGG GATTTTGGCT ATAGGATTTA TAAGATGCTG 
CTGAGCCTTC CTGAAAAGGT AGTGACTCCA CCTGAACCTG AGAAGGAGGA GCCAGCCAAG 
GAGGAAGCAG TTAAGGAGGA AGAAGTGGTC AAAGAGGAGG TGGCCAAGGA GTCGAAGGAT 
GAGGTACAGA ATGAGGGAGC AGCTGCTGAG TCAGACACCC CACTGAAGGA GGATGGACTT 
TTGCCCAAAC CCCCATCTTC TGGGGGAGAG GAAGAAGAGA AGCCCCGGGG TGAGGCATCT 
GAGGACCTTT GTGAGATGGC TCTGGACCCA GAACTGCTGC TTCTGAGGGA TGATGGAGAA 
GAGGAATTTG CAGGAGCAAA GCTGGAGGAT TCGGAGGTAC GGTCCGTTGC CTCAAACCAG 
TCAGAGATGG AATTCTCTTC ACTTCAGGAC ATGCCCAAGG AGCTGGATCC CTCTGCTGTG 
CTCCCCTTGG ATTGTCTCCT TGCTTTTGTG TTCTTTGATG CCAACTGGTG TGGCTACTTG 
CACCGGAGAG ACTTGGAGAG GATTCTACTT ACCCTTGGGC TACGGCTTAG TGCAGAGCAG 
GCCAAGCAGC TGGTCAGCAG GGTGGTGACC CAGAACATCT GCCAGTACCG GAGCCTTCAG 
TATAGCCGCC AGGAGGGCAT GGATGGCGGA CTTCCTGAGG AGGTGCTCTG TGGAAACCTT 
GATCTGCTAC CTCCTCCTGG GAAAAGCGCA AAGCCAAGTG CTGCCCCCAT GGAACACAAG 
GGCCTGGTGT CCCACAATGG CAGCCTAATC AATGTGGGGA ACCTGCTGCA GCGTGCAGAG 
CAGCAGGACA GTGGACGGCT CTACCTGGAG AACAAGATTC ACACACTGGA ACTGAAGCTG 
GAGGAGAGCC ATAACCGATT CTCAGCCACT GAAGTGACAA ATAAGACACT GGCGGCAGAA 
ATGCAGGAGT TGCGAAATCG GTTGGCTGAG GCTGAGGAGA CAGCCCGGAC AGCTGAACGA 
CAGAAGAACC AGCTTCAGCG GCTGCTACAG GACTTCCGAA GGCGCCTGAC CCCTCTGCAG 
CTTGAGATGC AGCGAATGGT TGAAAAGGCC GACAGCTGGG TAGAGAAGGA GGAGCCAGCA 
CCTACCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004382180.1
CDS	331..3102
Translation	MSQFKRQRINPLPGGRNFSGAASTSLLGPPPGLLTPPVATDLSQNARHLQGGEKQRVFTGIVTSLHDYFGVVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQGILGAQPQLIFQHHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYTVDSPICDFLELQRRYRSLLVPSDFLAVHLSWLSAFPLSQPFSLHHPSRIQVSSEKEPAPDTGAEPIPSDSDPGYSSKVLLLSSPGLEELYRCCMLFVDDIAEPRETPEHPLKQIKFLLGRKDEEAVLVGGEWSPSLDGLDPKADPQVLVRTAIRCAQVQTGIDLSSCTKWWRFAEFQYLQPGPPRRLQTVVVYLPDVWTIMPTLEEWEALCQQKAAEAALPPQESPVETESSEQAADASEQAADTSKQNAENPEVTAQQEMDTDLPEAPPPPLEPAVIARSDCINLSLHGIVEDRRPKERISFEVMVLAELFLEMLQRDFGYRIYKMLLSLPEKVVTPPEPEKEEPAKEEAVKEEEVVKEEVAKESKDEVQNEGAAAESDTPLKEDGLLPKPPSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGLRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGMDGGLPEEVLCGNLDLLPPPGKSAKPSAAPMEHKGLVSHNGSLINVGNLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRNRLAEAEETARTAERQKNQLQRLLQDFRRRLTPLQLEMQRMVEKADSWVEKEEPAPTN

Target ORF information:

RefSeq Version	XM_004382123.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris cell cycle and apoptosis regulator 2 (LOC101360776), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004382123.2

ORF Insert Sequence:

ATGTCCCAGT TTAAGCGCCA GCGGATCAAT CCGCTTCCAG GGGGACGCAA CTTTTCAGGC 
GCAGCTTCAA CATCTCTTCT GGGACCTCCT CCTGGTTTGC TCACTCCTCC TGTGGCTACA 
GATCTGTCCC AAAATGCCAG GCACCTTCAG GGTGGGGAGA AGCAGCGGGT CTTCACTGGA 
ATTGTTACCA GTTTGCACGA CTACTTTGGG GTGGTGGATG AAGAGGTTTT TTTTCAGCTA 
AGTGTGGTGA AGGGGCGGCT GCCCCAGCTG GGTGAGAAGG TGCTGGTGAA GGCTGCATAC 
AACCCAGGCC AGGCAGTGCC TTGGAATGCT GTCAAGGTGC AAACACTCTC CAACCAGCCC 
CTGCTGAAGT CGCCAGCACC TCCCCTTCTG CACGTGGCAG CCCTGGGCCA GAAGCAAGGC 
ATCCTGGGAG CTCAGCCTCA GTTGATCTTC CAGCATCACC GAATTCCCCC TCTCTTTCCT 
CAGAAGCCTC TGAGTCTCTT CCAAACATCC CACACTCTTC ACCTGAGCCA CTTGAACAGA 
TTTCCTGCTC GGGGTCCTCA TGGAAGATTG GATCAGGGCC GAAGTGATGA CTATGACTCC 
AAGAAACGTA AACAACGGGC TGGCGGAGAG CCTTGGGGTG CCAAGAAACC AAGGCATGAC 
CTGCCTCCTT ACCGGGTCCA CCTCACTCCC TATACTGTGG ACAGCCCCAT CTGTGACTTC 
TTAGAACTCC AGCGCCGTTA CCGCAGCCTC CTGGTTCCCT CAGATTTTCT GGCTGTTCAT 
CTGAGCTGGC TGTCAGCCTT CCCTCTGAGC CAGCCCTTTT CCCTCCACCA TCCAAGCCGT 
ATCCAGGTAT CTTCTGAGAA GGAGCCAGCT CCAGACACTG GTGCTGAGCC TATCCCTTCA 
GACAGTGACC CCGGATATAG TTCCAAGGTA CTGTTGCTCT CTTCCCCTGG ATTGGAGGAA 
TTGTATCGTT GTTGTATGCT GTTTGTGGAT GACATCGCTG AGCCGAGGGA GACGCCAGAA 
CATCCTTTGA AGCAGATTAA GTTTTTGCTG GGCCGGAAAG ATGAAGAGGC AGTGCTGGTT 
GGGGGCGAGT GGTCTCCTTC CTTGGATGGC CTTGACCCCA AGGCTGACCC GCAGGTGCTA 
GTCCGCACCG CGATCCGGTG TGCACAGGTT CAGACTGGCA TTGACTTGAG CAGCTGCACA 
AAGTGGTGGC GCTTTGCTGA GTTTCAGTAC CTGCAGCCGG GACCCCCAAG GCGGCTCCAG 
ACAGTGGTGG TGTACCTGCC GGATGTCTGG ACCATTATGC CTACTTTGGA AGAGTGGGAG 
GCCCTGTGCC AGCAGAAAGC TGCAGAGGCA GCTCTCCCAC CGCAGGAGTC ACCAGTGGAA 
ACAGAGTCTT CTGAACAGGC GGCAGATGCA TCGGAGCAAG CAGCAGACAC CTCTAAACAA 
AATGCAGAGA ATCCGGAGGT CACCGCGCAG CAGGAAATGG ACACAGATCT CCCAGAGGCC 
CCTCCACCTC CTCTAGAACC TGCTGTCATT GCACGCTCTG ACTGTATAAA CCTCTCTCTC 
CATGGTATTG TGGAGGATCG GAGGCCAAAA GAAAGAATCT CTTTTGAGGT GATGGTGTTG 
GCTGAGCTAT TTCTGGAGAT GCTGCAGAGG GATTTTGGCT ATAGGATTTA TAAGATGCTG 
CTGAGCCTTC CTGAAAAGGT AGTGACTCCA CCTGAACCTG AGAAGGAGGA GCCAGCCAAG 
GAGGAAGCAG TTAAGGAGGA AGAAGTGGTC AAAGAGGAGG TGGCCAAGGA GTCGAAGGAT 
GAGGTACAGA ATGAGGGAGC AGCTGCTGAG TCAGACACCC CACTGAAGGA GGATGGACTT 
TTGCCCAAAC CCCCATCTTC TGGGGGAGAG GAAGAAGAGA AGCCCCGGGG TGAGGCATCT 
GAGGACCTTT GTGAGATGGC TCTGGACCCA GAACTGCTGC TTCTGAGGGA TGATGGAGAA 
GAGGAATTTG CAGGAGCAAA GCTGGAGGAT TCGGAGGTAC GGTCCGTTGC CTCAAACCAG 
TCAGAGATGG AATTCTCTTC ACTTCAGGAC ATGCCCAAGG AGCTGGATCC CTCTGCTGTG 
CTCCCCTTGG ATTGTCTCCT TGCTTTTGTG TTCTTTGATG CCAACTGGTG TGGCTACTTG 
CACCGGAGAG ACTTGGAGAG GATTCTACTT ACCCTTGGGC TACGGCTTAG TGCAGAGCAG 
GCCAAGCAGC TGGTCAGCAG GGTGGTGACC CAGAACATCT GCCAGTACCG GAGCCTTCAG 
TATAGCCGCC AGGAGGGCAT GGATGGCGGA CTTCCTGAGG AGGTGCTCTG TGGAAACCTT 
GATCTGCTAC CTCCTCCTGG GAAAAGCGCA AAGCCAAGTG CTGCCCCCAT GGAACACAAG 
GGCCTGGTGT CCCACAATGG CAGCCTAATC AATGTGGGGA ACCTGCTGCA GCGTGCAGAG 
CAGCAGGACA GTGGACGGCT CTACCTGGAG AACAAGATTC ACACACTGGA ACTGAAGCTG 
GAGGAGAGCC ATAACCGATT CTCAGCCACT GAAGTGACAA ATAAGACACT GGCGGCAGAA 
ATGCAGGAGT TGCGAAATCG GTTGGCTGAG GCTGAGGAGA CAGCCCGGAC AGCTGAACGA 
CAGAAGAACC AGCTTCAGCG GCTGCTACAG GACTTCCGAA GGCGCCTGAC CCCTCTGCAG 
CTTGAGATGC AGCGAATGGT TGAAAAGGCC GACAGCTGGG TAGAGAAGGA GGAGCCAGCA 
CCTACCAACT GA