LOC101353361 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101353361
Entrez Gene ID	101353361
Full Name	polycystin 2 like 1, transient receptor potential cation channel
Synonyms	PKD2L1
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004370140.2	XP_004370197.1	polycystic kidney disease 2-like 1 protein
XM_004370140.1	XP_004370197.1	polycystic kidney disease 2-like 1 protein

Pathways from BioSystems
Gene Ontology

The following LOC101353361 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101353361 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr165969	XM_004370140.2 Latest version!	Trichechus manatus latirostris polycystin 2 like 1, transient receptor potential cation channel (LOC101353361), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
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OTr165969	XM_004370140.1	Trichechus manatus latirostris polycystic kidney disease 2-like 1 (LOC101353361), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101353361 ( XM_004370140.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004370197.1 Trichechus manatus latirostris polycystin 2 like 1, transient receptor potential cation channel (LOC101353361), mRNA.

Price & Availability↑

CloneID	OTr165969
Clone ID Related Accession (Same CDS sequence)	XM_004370140.2 , XM_004370140.1
Accession Version	XM_004370140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	polycystic kidney disease 2-like 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443943.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004370140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCTA TGGAAAGTCC TGAAGGGCAG GAACTGCAAA ATCTGGGGAA CGGAGCCTGG 
GAAAATCCTG CCTACAGCAG TCCCCCTTCT CCATATGGGA CACAGAGGAT CTGCACCATC 
TCCAGTGTGG TCCCCCCCCA GCCTCAACCC AAGATGCCTG AAGATGGACC CCAGGAGAAG 
GCACACAGGA CCCTAGTGTC CAGCTGCTGC CTCCATATCC GTCGAAGTGT CAGAGGACTG 
TGGGGAACAA CTCTGACTGA GAACACAGCT GAGAACCCGG AACTTTATGT CAAGACCACC 
CTGCGGGAGC TTTTGGTGTA TATCGTGTTC CTGGTGGACA TCTGTCTATT GACTTACGGA 
ATGACAAGCT CCAGTGCCTA TTACTACACC AAAATGATGT CTGAGCTCTT CTTACATACT 
CCGTCAGACA CTGGTGTCTC CTTCCAGACC ATCAGCAGCA TGGCGGACTT CTGGGATTTT 
GCCCAGGGCC CACTCCTGGA CAGTTTGTAT TGGACCAAAT GGTACAACAA CCAGAGCCTC 
GGCCACGGCT CCCACTCCTT CATCTACTAC GAGAACCTGC TGCTGGGGGT TCCAAGGCTG 
CGGCAGCTGA GGGTCCGCAA TGACTCCTGT GTGGTGCATA AGGACTTCCG TGAGGACATT 
TTGAGCTGCT ACGATGTCTA CTCTCCGGAT AAGGAAGAGC AGCTCCCCTT TGGACCCCTC 
AATGGCACAG CGTGGACATA CCACTCACAG GATGAGCTGG GGGGCTCCTC CCACTGGGGC 
CAGCTCACAA GCTATAGTGG AGGTGGCTAC TACCTGGACC TCCTGGGATC TCGACAAGCC 
AGTGCAGAGA CTCTCCGGGA CCTACAAGAG GGCCTGTGGC TGGACCGGGG CACTCGTGTG 
GTCTTCATTG ACTTCTCAGT CTACAATGCC AACATCAATC TTTTCTGTGT TTTGAGGCTG 
GTGGTGGAGT TTCCAGCTAC AGGAGGTGCG ATTCCATCCT GGCAGATCCG CACAGTGAAG 
CTGATCCGCT ACGTCAGTAA CTGGGACTTC TTTATCATTG GCTGTGAGAT CATTTTCTGT 
GTCTTCATCT TCTACTATGT GGTGGAGGAG ATCTTGGAGC TCCACATCCA CCACCTTCGC 
TACCTCAGCA GCATCTGGAA CATCCTAGAC CTGGTTGTCA TCTTGCTCTC CATTGTGGCT 
GTGGGTTTCC ACATATTTCG AACACTCGAG GTGAATCGAC TGATGGGGAA GCTCCTGCAG 
CAGCCAAACA CATACGCTGA CTTCGAGTTC CTCGCCTTCT GGCAGACACA GTACAACAAC 
ATGAATGCTG TCAACCTCTT CTTTGCCTGG ATCAAGATAT TCAAGTACAT CAGCTTCAAC 
AAAACCATGA CTCAACTCTC CTCCACGCTG GCCCGCTGTG CCAAGGACAT CCTGGGCTTC 
GCCGTCATGT TCTTCATCGT TTTTTTCGCT TATGCCCAAC TCGGCTACCT ACTTTTCGGG 
ACCCAAGTGG AAAACTTCAG CACTTTCATC AAGTGCATTT TCACTCAGTT CCGGATTATC 
CTTGGGGACT TTGACTACAA TGCTATTGAC AATGCCAACC GCATCCTGGG TCCTGCCTAC 
TTTGTTACCT ACGTCTTCTT CGTCTTCTTT GTGCTCCTGA ACATGTTCCT GGCCATCATC 
AATGACACCT ATTCAGAGGT CAAGGAGGAG CTGGCTGGAC AGAAGGATGA GCTGCAGCTT 
TCTGACCTTC TGAAACAGGG CTACAATAAG ACCTTACTGA GGCTGCGTCT GAGGAAGGAG 
CGAGTTTCAG ATATGCAGAA GGTCCTGCAA GGTGGGGAGC AGGAGATCCA GTTTGAGGAT 
TTCACCAACA CCTTGCGGGA ACTGGGACAT GCACAGCATG AAATCACAGA GCTCACAGCT 
GCCTTTACCA GGTTTGACCA AGACGGGAAT TGCATCCTGG ATGAGAAGGA GCAGGCACAA 
ATGCGACAGA ATCTGGAGGA GGAGAGGGTG ACCCTCAGTG CTGAGATTGA GAACTTGGGC 
CAGTGCACTG TGAGTAGTCC ACCAGGAGAG TCGGGCTCAG AGGCTGCCAG AGCAGGTGGC 
TGGGTTTCAG GAGAAGAATT CCACACACTG ACAAGGAGGG TTCTGCAGCT GGAGACTGTC 
TTAGAAGGAG TCGTGTCCCG GGTTGAATCT GTGGGCTCAA AGCTGAAGAT GCTGGAAAGG 
AAGGGGCACC TGGCTCTCTC CCCAGGCCTG GAGGAACATG CCATTTGGGA GCATCTGCAG 
CCAGGCCTAG CTGTGACCCC AGCTCCCTGG GGAGTCCAGG GTGGGCAGGA GAGTGAGCTT 
CCCTGTAAAC AAGAAGAAGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004370197.1
CDS	380..2746
Translation	MNAMESPEGQELQNLGNGAWENPAYSSPPSPYGTQRICTISSVVPPQPQPKMPEDGPQEKAHRTLVSSCCLHIRRSVRGLWGTTLTENTAENPELYVKTTLRELLVYIVFLVDICLLTYGMTSSSAYYYTKMMSELFLHTPSDTGVSFQTISSMADFWDFAQGPLLDSLYWTKWYNNQSLGHGSHSFIYYENLLLGVPRLRQLRVRNDSCVVHKDFREDILSCYDVYSPDKEEQLPFGPLNGTAWTYHSQDELGGSSHWGQLTSYSGGGYYLDLLGSRQASAETLRDLQEGLWLDRGTRVVFIDFSVYNANINLFCVLRLVVEFPATGGAIPSWQIRTVKLIRYVSNWDFFIIGCEIIFCVFIFYYVVEEILELHIHHLRYLSSIWNILDLVVILLSIVAVGFHIFRTLEVNRLMGKLLQQPNTYADFEFLAFWQTQYNNMNAVNLFFAWIKIFKYISFNKTMTQLSSTLARCAKDILGFAVMFFIVFFAYAQLGYLLFGTQVENFSTFIKCIFTQFRIILGDFDYNAIDNANRILGPAYFVTYVFFVFFVLLNMFLAIINDTYSEVKEELAGQKDELQLSDLLKQGYNKTLLRLRLRKERVSDMQKVLQGGEQEIQFEDFTNTLRELGHAQHEITELTAAFTRFDQDGNCILDEKEQAQMRQNLEEERVTLSAEIENLGQCTVSSPPGESGSEAARAGGWVSGEEFHTLTRRVLQLETVLEGVVSRVESVGSKLKMLERKGHLALSPGLEEHAIWEHLQPGLAVTPAPWGVQGGQESELPCKQEEEE

Target ORF information:

RefSeq Version	XM_004370140.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris polycystin 2 like 1, transient receptor potential cation channel (LOC101353361), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004370140.2

ORF Insert Sequence:

ATGAATGCTA TGGAAAGTCC TGAAGGGCAG GAACTGCAAA ATCTGGGGAA CGGAGCCTGG 
GAAAATCCTG CCTACAGCAG TCCCCCTTCT CCATATGGGA CACAGAGGAT CTGCACCATC 
TCCAGTGTGG TCCCCCCCCA GCCTCAACCC AAGATGCCTG AAGATGGACC CCAGGAGAAG 
GCACACAGGA CCCTAGTGTC CAGCTGCTGC CTCCATATCC GTCGAAGTGT CAGAGGACTG 
TGGGGAACAA CTCTGACTGA GAACACAGCT GAGAACCCGG AACTTTATGT CAAGACCACC 
CTGCGGGAGC TTTTGGTGTA TATCGTGTTC CTGGTGGACA TCTGTCTATT GACTTACGGA 
ATGACAAGCT CCAGTGCCTA TTACTACACC AAAATGATGT CTGAGCTCTT CTTACATACT 
CCGTCAGACA CTGGTGTCTC CTTCCAGACC ATCAGCAGCA TGGCGGACTT CTGGGATTTT 
GCCCAGGGCC CACTCCTGGA CAGTTTGTAT TGGACCAAAT GGTACAACAA CCAGAGCCTC 
GGCCACGGCT CCCACTCCTT CATCTACTAC GAGAACCTGC TGCTGGGGGT TCCAAGGCTG 
CGGCAGCTGA GGGTCCGCAA TGACTCCTGT GTGGTGCATA AGGACTTCCG TGAGGACATT 
TTGAGCTGCT ACGATGTCTA CTCTCCGGAT AAGGAAGAGC AGCTCCCCTT TGGACCCCTC 
AATGGCACAG CGTGGACATA CCACTCACAG GATGAGCTGG GGGGCTCCTC CCACTGGGGC 
CAGCTCACAA GCTATAGTGG AGGTGGCTAC TACCTGGACC TCCTGGGATC TCGACAAGCC 
AGTGCAGAGA CTCTCCGGGA CCTACAAGAG GGCCTGTGGC TGGACCGGGG CACTCGTGTG 
GTCTTCATTG ACTTCTCAGT CTACAATGCC AACATCAATC TTTTCTGTGT TTTGAGGCTG 
GTGGTGGAGT TTCCAGCTAC AGGAGGTGCG ATTCCATCCT GGCAGATCCG CACAGTGAAG 
CTGATCCGCT ACGTCAGTAA CTGGGACTTC TTTATCATTG GCTGTGAGAT CATTTTCTGT 
GTCTTCATCT TCTACTATGT GGTGGAGGAG ATCTTGGAGC TCCACATCCA CCACCTTCGC 
TACCTCAGCA GCATCTGGAA CATCCTAGAC CTGGTTGTCA TCTTGCTCTC CATTGTGGCT 
GTGGGTTTCC ACATATTTCG AACACTCGAG GTGAATCGAC TGATGGGGAA GCTCCTGCAG 
CAGCCAAACA CATACGCTGA CTTCGAGTTC CTCGCCTTCT GGCAGACACA GTACAACAAC 
ATGAATGCTG TCAACCTCTT CTTTGCCTGG ATCAAGATAT TCAAGTACAT CAGCTTCAAC 
AAAACCATGA CTCAACTCTC CTCCACGCTG GCCCGCTGTG CCAAGGACAT CCTGGGCTTC 
GCCGTCATGT TCTTCATCGT TTTTTTCGCT TATGCCCAAC TCGGCTACCT ACTTTTCGGG 
ACCCAAGTGG AAAACTTCAG CACTTTCATC AAGTGCATTT TCACTCAGTT CCGGATTATC 
CTTGGGGACT TTGACTACAA TGCTATTGAC AATGCCAACC GCATCCTGGG TCCTGCCTAC 
TTTGTTACCT ACGTCTTCTT CGTCTTCTTT GTGCTCCTGA ACATGTTCCT GGCCATCATC 
AATGACACCT ATTCAGAGGT CAAGGAGGAG CTGGCTGGAC AGAAGGATGA GCTGCAGCTT 
TCTGACCTTC TGAAACAGGG CTACAATAAG ACCTTACTGA GGCTGCGTCT GAGGAAGGAG 
CGAGTTTCAG ATATGCAGAA GGTCCTGCAA GGTGGGGAGC AGGAGATCCA GTTTGAGGAT 
TTCACCAACA CCTTGCGGGA ACTGGGACAT GCACAGCATG AAATCACAGA GCTCACAGCT 
GCCTTTACCA GGTTTGACCA AGACGGGAAT TGCATCCTGG ATGAGAAGGA GCAGGCACAA 
ATGCGACAGA ATCTGGAGGA GGAGAGGGTG ACCCTCAGTG CTGAGATTGA GAACTTGGGC 
CAGTGCACTG TGAGTAGTCC ACCAGGAGAG TCGGGCTCAG AGGCTGCCAG AGCAGGTGGC 
TGGGTTTCAG GAGAAGAATT CCACACACTG ACAAGGAGGG TTCTGCAGCT GGAGACTGTC 
TTAGAAGGAG TCGTGTCCCG GGTTGAATCT GTGGGCTCAA AGCTGAAGAT GCTGGAAAGG 
AAGGGGCACC TGGCTCTCTC CCCAGGCCTG GAGGAACATG CCATTTGGGA GCATCTGCAG 
CCAGGCCTAG CTGTGACCCC AGCTCCCTGG GGAGTCCAGG GTGGGCAGGA GAGTGAGCTT 
CCCTGTAAAC AAGAAGAAGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101353361 ( XM_004370140.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004370197.1 Trichechus manatus latirostris polycystic kidney disease 2-like 1 (LOC101353361), mRNA.

Price & Availability↑

CloneID	OTr165969
Clone ID Related Accession (Same CDS sequence)	XM_004370140.2 , XM_004370140.1
Accession Version	XM_004370140.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	polycystic kidney disease 2-like 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443943.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAATGCTA TGGAAAGTCC TGAAGGGCAG GAACTGCAAA ATCTGGGGAA CGGAGCCTGG 
GAAAATCCTG CCTACAGCAG TCCCCCTTCT CCATATGGGA CACAGAGGAT CTGCACCATC 
TCCAGTGTGG TCCCCCCCCA GCCTCAACCC AAGATGCCTG AAGATGGACC CCAGGAGAAG 
GCACACAGGA CCCTAGTGTC CAGCTGCTGC CTCCATATCC GTCGAAGTGT CAGAGGACTG 
TGGGGAACAA CTCTGACTGA GAACACAGCT GAGAACCCGG AACTTTATGT CAAGACCACC 
CTGCGGGAGC TTTTGGTGTA TATCGTGTTC CTGGTGGACA TCTGTCTATT GACTTACGGA 
ATGACAAGCT CCAGTGCCTA TTACTACACC AAAATGATGT CTGAGCTCTT CTTACATACT 
CCGTCAGACA CTGGTGTCTC CTTCCAGACC ATCAGCAGCA TGGCGGACTT CTGGGATTTT 
GCCCAGGGCC CACTCCTGGA CAGTTTGTAT TGGACCAAAT GGTACAACAA CCAGAGCCTC 
GGCCACGGCT CCCACTCCTT CATCTACTAC GAGAACCTGC TGCTGGGGGT TCCAAGGCTG 
CGGCAGCTGA GGGTCCGCAA TGACTCCTGT GTGGTGCATA AGGACTTCCG TGAGGACATT 
TTGAGCTGCT ACGATGTCTA CTCTCCGGAT AAGGAAGAGC AGCTCCCCTT TGGACCCCTC 
AATGGCACAG CGTGGACATA CCACTCACAG GATGAGCTGG GGGGCTCCTC CCACTGGGGC 
CAGCTCACAA GCTATAGTGG AGGTGGCTAC TACCTGGACC TCCTGGGATC TCGACAAGCC 
AGTGCAGAGA CTCTCCGGGA CCTACAAGAG GGCCTGTGGC TGGACCGGGG CACTCGTGTG 
GTCTTCATTG ACTTCTCAGT CTACAATGCC AACATCAATC TTTTCTGTGT TTTGAGGCTG 
GTGGTGGAGT TTCCAGCTAC AGGAGGTGCG ATTCCATCCT GGCAGATCCG CACAGTGAAG 
CTGATCCGCT ACGTCAGTAA CTGGGACTTC TTTATCATTG GCTGTGAGAT CATTTTCTGT 
GTCTTCATCT TCTACTATGT GGTGGAGGAG ATCTTGGAGC TCCACATCCA CCACCTTCGC 
TACCTCAGCA GCATCTGGAA CATCCTAGAC CTGGTTGTCA TCTTGCTCTC CATTGTGGCT 
GTGGGTTTCC ACATATTTCG AACACTCGAG GTGAATCGAC TGATGGGGAA GCTCCTGCAG 
CAGCCAAACA CATACGCTGA CTTCGAGTTC CTCGCCTTCT GGCAGACACA GTACAACAAC 
ATGAATGCTG TCAACCTCTT CTTTGCCTGG ATCAAGATAT TCAAGTACAT CAGCTTCAAC 
AAAACCATGA CTCAACTCTC CTCCACGCTG GCCCGCTGTG CCAAGGACAT CCTGGGCTTC 
GCCGTCATGT TCTTCATCGT TTTTTTCGCT TATGCCCAAC TCGGCTACCT ACTTTTCGGG 
ACCCAAGTGG AAAACTTCAG CACTTTCATC AAGTGCATTT TCACTCAGTT CCGGATTATC 
CTTGGGGACT TTGACTACAA TGCTATTGAC AATGCCAACC GCATCCTGGG TCCTGCCTAC 
TTTGTTACCT ACGTCTTCTT CGTCTTCTTT GTGCTCCTGA ACATGTTCCT GGCCATCATC 
AATGACACCT ATTCAGAGGT CAAGGAGGAG CTGGCTGGAC AGAAGGATGA GCTGCAGCTT 
TCTGACCTTC TGAAACAGGG CTACAATAAG ACCTTACTGA GGCTGCGTCT GAGGAAGGAG 
CGAGTTTCAG ATATGCAGAA GGTCCTGCAA GGTGGGGAGC AGGAGATCCA GTTTGAGGAT 
TTCACCAACA CCTTGCGGGA ACTGGGACAT GCACAGCATG AAATCACAGA GCTCACAGCT 
GCCTTTACCA GGTTTGACCA AGACGGGAAT TGCATCCTGG ATGAGAAGGA GCAGGCACAA 
ATGCGACAGA ATCTGGAGGA GGAGAGGGTG ACCCTCAGTG CTGAGATTGA GAACTTGGGC 
CAGTGCACTG TGAGTAGTCC ACCAGGAGAG TCGGGCTCAG AGGCTGCCAG AGCAGGTGGC 
TGGGTTTCAG GAGAAGAATT CCACACACTG ACAAGGAGGG TTCTGCAGCT GGAGACTGTC 
TTAGAAGGAG TCGTGTCCCG GGTTGAATCT GTGGGCTCAA AGCTGAAGAT GCTGGAAAGG 
AAGGGGCACC TGGCTCTCTC CCCAGGCCTG GAGGAACATG CCATTTGGGA GCATCTGCAG 
CCAGGCCTAG CTGTGACCCC AGCTCCCTGG GGAGTCCAGG GTGGGCAGGA GAGTGAGCTT 
CCCTGTAAAC AAGAAGAAGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004370197.1
CDS	380..2746
Translation	MNAMESPEGQELQNLGNGAWENPAYSSPPSPYGTQRICTISSVVPPQPQPKMPEDGPQEKAHRTLVSSCCLHIRRSVRGLWGTTLTENTAENPELYVKTTLRELLVYIVFLVDICLLTYGMTSSSAYYYTKMMSELFLHTPSDTGVSFQTISSMADFWDFAQGPLLDSLYWTKWYNNQSLGHGSHSFIYYENLLLGVPRLRQLRVRNDSCVVHKDFREDILSCYDVYSPDKEEQLPFGPLNGTAWTYHSQDELGGSSHWGQLTSYSGGGYYLDLLGSRQASAETLRDLQEGLWLDRGTRVVFIDFSVYNANINLFCVLRLVVEFPATGGAIPSWQIRTVKLIRYVSNWDFFIIGCEIIFCVFIFYYVVEEILELHIHHLRYLSSIWNILDLVVILLSIVAVGFHIFRTLEVNRLMGKLLQQPNTYADFEFLAFWQTQYNNMNAVNLFFAWIKIFKYISFNKTMTQLSSTLARCAKDILGFAVMFFIVFFAYAQLGYLLFGTQVENFSTFIKCIFTQFRIILGDFDYNAIDNANRILGPAYFVTYVFFVFFVLLNMFLAIINDTYSEVKEELAGQKDELQLSDLLKQGYNKTLLRLRLRKERVSDMQKVLQGGEQEIQFEDFTNTLRELGHAQHEITELTAAFTRFDQDGNCILDEKEQAQMRQNLEEERVTLSAEIENLGQCTVSSPPGESGSEAARAGGWVSGEEFHTLTRRVLQLETVLEGVVSRVESVGSKLKMLERKGHLALSPGLEEHAIWEHLQPGLAVTPAPWGVQGGQESELPCKQEEEE

Target ORF information:

RefSeq Version	XM_004370140.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris polycystic kidney disease 2-like 1 (LOC101353361), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004370140.1

ORF Insert Sequence:

ATGAATGCTA TGGAAAGTCC TGAAGGGCAG GAACTGCAAA ATCTGGGGAA CGGAGCCTGG 
GAAAATCCTG CCTACAGCAG TCCCCCTTCT CCATATGGGA CACAGAGGAT CTGCACCATC 
TCCAGTGTGG TCCCCCCCCA GCCTCAACCC AAGATGCCTG AAGATGGACC CCAGGAGAAG 
GCACACAGGA CCCTAGTGTC CAGCTGCTGC CTCCATATCC GTCGAAGTGT CAGAGGACTG 
TGGGGAACAA CTCTGACTGA GAACACAGCT GAGAACCCGG AACTTTATGT CAAGACCACC 
CTGCGGGAGC TTTTGGTGTA TATCGTGTTC CTGGTGGACA TCTGTCTATT GACTTACGGA 
ATGACAAGCT CCAGTGCCTA TTACTACACC AAAATGATGT CTGAGCTCTT CTTACATACT 
CCGTCAGACA CTGGTGTCTC CTTCCAGACC ATCAGCAGCA TGGCGGACTT CTGGGATTTT 
GCCCAGGGCC CACTCCTGGA CAGTTTGTAT TGGACCAAAT GGTACAACAA CCAGAGCCTC 
GGCCACGGCT CCCACTCCTT CATCTACTAC GAGAACCTGC TGCTGGGGGT TCCAAGGCTG 
CGGCAGCTGA GGGTCCGCAA TGACTCCTGT GTGGTGCATA AGGACTTCCG TGAGGACATT 
TTGAGCTGCT ACGATGTCTA CTCTCCGGAT AAGGAAGAGC AGCTCCCCTT TGGACCCCTC 
AATGGCACAG CGTGGACATA CCACTCACAG GATGAGCTGG GGGGCTCCTC CCACTGGGGC 
CAGCTCACAA GCTATAGTGG AGGTGGCTAC TACCTGGACC TCCTGGGATC TCGACAAGCC 
AGTGCAGAGA CTCTCCGGGA CCTACAAGAG GGCCTGTGGC TGGACCGGGG CACTCGTGTG 
GTCTTCATTG ACTTCTCAGT CTACAATGCC AACATCAATC TTTTCTGTGT TTTGAGGCTG 
GTGGTGGAGT TTCCAGCTAC AGGAGGTGCG ATTCCATCCT GGCAGATCCG CACAGTGAAG 
CTGATCCGCT ACGTCAGTAA CTGGGACTTC TTTATCATTG GCTGTGAGAT CATTTTCTGT 
GTCTTCATCT TCTACTATGT GGTGGAGGAG ATCTTGGAGC TCCACATCCA CCACCTTCGC 
TACCTCAGCA GCATCTGGAA CATCCTAGAC CTGGTTGTCA TCTTGCTCTC CATTGTGGCT 
GTGGGTTTCC ACATATTTCG AACACTCGAG GTGAATCGAC TGATGGGGAA GCTCCTGCAG 
CAGCCAAACA CATACGCTGA CTTCGAGTTC CTCGCCTTCT GGCAGACACA GTACAACAAC 
ATGAATGCTG TCAACCTCTT CTTTGCCTGG ATCAAGATAT TCAAGTACAT CAGCTTCAAC 
AAAACCATGA CTCAACTCTC CTCCACGCTG GCCCGCTGTG CCAAGGACAT CCTGGGCTTC 
GCCGTCATGT TCTTCATCGT TTTTTTCGCT TATGCCCAAC TCGGCTACCT ACTTTTCGGG 
ACCCAAGTGG AAAACTTCAG CACTTTCATC AAGTGCATTT TCACTCAGTT CCGGATTATC 
CTTGGGGACT TTGACTACAA TGCTATTGAC AATGCCAACC GCATCCTGGG TCCTGCCTAC 
TTTGTTACCT ACGTCTTCTT CGTCTTCTTT GTGCTCCTGA ACATGTTCCT GGCCATCATC 
AATGACACCT ATTCAGAGGT CAAGGAGGAG CTGGCTGGAC AGAAGGATGA GCTGCAGCTT 
TCTGACCTTC TGAAACAGGG CTACAATAAG ACCTTACTGA GGCTGCGTCT GAGGAAGGAG 
CGAGTTTCAG ATATGCAGAA GGTCCTGCAA GGTGGGGAGC AGGAGATCCA GTTTGAGGAT 
TTCACCAACA CCTTGCGGGA ACTGGGACAT GCACAGCATG AAATCACAGA GCTCACAGCT 
GCCTTTACCA GGTTTGACCA AGACGGGAAT TGCATCCTGG ATGAGAAGGA GCAGGCACAA 
ATGCGACAGA ATCTGGAGGA GGAGAGGGTG ACCCTCAGTG CTGAGATTGA GAACTTGGGC 
CAGTGCACTG TGAGTAGTCC ACCAGGAGAG TCGGGCTCAG AGGCTGCCAG AGCAGGTGGC 
TGGGTTTCAG GAGAAGAATT CCACACACTG ACAAGGAGGG TTCTGCAGCT GGAGACTGTC 
TTAGAAGGAG TCGTGTCCCG GGTTGAATCT GTGGGCTCAA AGCTGAAGAT GCTGGAAAGG 
AAGGGGCACC TGGCTCTCTC CCCAGGCCTG GAGGAACATG CCATTTGGGA GCATCTGCAG 
CCAGGCCTAG CTGTGACCCC AGCTCCCTGG GGAGTCCAGG GTGGGCAGGA GAGTGAGCTT 
CCCTGTAAAC AAGAAGAAGA GGAGTGA