LOC101343034 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101343034
Entrez Gene ID	101343034
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Synonyms	AGL
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004385714.3	XP_004385771.1	glycogen debranching enzyme isoform X1
XM_023739979.1	XP_023595747.1	glycogen debranching enzyme isoform X1
XM_004385714.2	XP_004385771.1	glycogen debranching enzyme
XM_023739982.1	XP_023595750.1	glycogen debranching enzyme isoform X1
XM_023739981.1	XP_023595749.1	glycogen debranching enzyme isoform X1
XM_023739983.1	XP_023595751.1	glycogen debranching enzyme isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101343034 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101343034 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr224946	XM_023739983.1 Latest version!	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTr183577	XM_023739981.1 Latest version!	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OTr183577	XM_023739982.1 Latest version!	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OTr183577	XM_004385714.3 Latest version!	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OTr183577	XM_023739979.1 Latest version!	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OTr183577	XM_004385714.2	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101343034 ( XM_023739983.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023595751.1 Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTr224946
Clone ID Related Accession (Same CDS sequence)	XM_023739983.1
Accession Version	XM_023739983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCCT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CTAAAGAATC AGGTTACAAC 
ATGATTCATT TTACCCCATT GCAGACTCTT GGAGTATCTA GGTCATGCTA CTCTCTGGCT 
GATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTGATC GAAAATATAG CTGGAATGAT 
GTTGGACAGC TAGTGGAAAA ATTGAAAAAG GAATGGAATA TTCTTTGTAT TACTGATGTT 
GTCTACAATC ATACTGCTGC TAACAGTAAA TGGATCCATG AGCATCCAGA GTGTGCATAT 
AACCTCGTGA ATTCTCCACA CTTGAAACCT GCCTGGCTCT TAGACAGAGC ACTTTGGCAT 
TTATCCCGTG ATGTTGCAGA AGGAAAATAC AAAGAAAAAG GATTACCTCC TCTGATTGAA 
AATGATCATC ATTTGAATTG CTTTCGAAAA ATAGTTTGGG AGGATGTTTT TCCAAAGATT 
CATCTCTGGG AATTTTTCCA AGTAGATGTT TACAAAACAG TTGAGCAATT TAGAAGACTT 
CTTACACAAG AACATAGGGA AATAACGAAG TCTGATCCAA TGCAACATCT TAAGATTATT 
CAAGATCCTG AATACCGACG GCTTGGCTGT ACAGTAGATA TGAACATTGC ACTAGCAACT 
TTCATCCCAC ATGACAAAGG GCCAGCTGCA ATTGATGAGT GCTGTAATTG GTTCCATAAG 
AGACTTGAGG AATTAAATTC AGAGAAGCAT CAACTTGTGA ACTATCATCA GGAACAGGCT 
GTTAATTGCC TTTTGGGAAA TGTTTTTTAT GAACGACTGG CTGACCACGG TCCTAAAGTA 
GGACCTGTCA CTAGAAAATA TCCTTTAGTT ACCAGATATT TTACTTTCCC ATTTGAAGAA 
ATGACCCTTT CTGCAGAAGA ATCTATGATT CATCTTCCAG ATAAAGCTTG TTTTCTGATG 
GCACACAATG GATGGGTAAT GGGAGATGAT CCCCTTCGAA ACTTTGCTGA ACCAGGTTCA 
GACGTATACC TAAGGAGAGA ACTTATATGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
AATAAACCAG AGGACTGTCC TTATCTCTGG GCACATATGA AAAAATACAC TGAAATAGCT 
GCAACTTACT TCCAGGGACT ACGTCTTGAT AACTGCCACT CAACACCTCT TCGTGTAGCT 
GAGTACATGT TGGATGCTGC TAGGAAATTG CAACCCAATT TATATGTAGT AGCCGAACTG 
TTCACAGGAA GTGAAGACCT GGACAATATC TTTGTTACCA GACTGGGCAT TAGTTCCTTA 
ATAAGAGAGG CAATGAGTGC ATATGATAGT CATGAAGAGG GCAGGTTAGT TTACCGATAT 
GGAGGAGAAC CTGTTGGATC CTTTGTTCAA CCCTGTTTGA GGCCTTTAGT GCCAACTATT 
GCACATGCCC TGTTTATGGA CATTACCCAT GACAATGAAT GTCCTATTGT GCATAGATCA 
GCATATGATG CTCTTCCAAG TTCCACAATT GTTTCTATGG CGTCTTGTGC TAGTGGAAGT 
ACTAAAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGCTT 
TACACTAAGT GGAATCCTGA AGCATCGCCA TCCACCACAG GTGAAGTTAA TTTCCAAAGT 
GGAATTATCG CGGCCCGATG CGCTGTCAAT AGACTTCACC AGGAGCTTGG GGCCAAGGGA 
TTTATTCAGG TGTATGTGGA TCAGGTTGAT GAAGACATAG TGGCAGTAAC AAGACATTCA 
CCTAGTATCC ACCAGTCTGT TGTGGCTGTG TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGTATCCCTG GCAAAATTGA AGAAGTAGTT 
CTTGAAGCTA GAACTATTGA GAGAAATACA AAACCTTATA GGAAGGATGA GAATTCAATC 
AATGGAATGC CAAATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAG TGAAAGTAAA 
ATTGTTAAAC AAGCTGGAGT TACCAAAAAA GGACCCAATG AATATATTCA GGAAATAGAA 
TTTGAAAACT TGTCTCCAGG AAGTATTATT GTATTCAGAG TGAGCCTTGA CCCACATGCA 
CAAGTTGCTG TTGGAATTCT TCGAAATCAT CTGATACAAT TCAGTTCTCA CTTTAAAGCT 
GGGAGCTTTG CTGTGGACAG CTCAGATCCT ATATTAAAAA TACCTTTTGC TTCTATTGCC 
TCTAAATTAA CTTTGGCTGA GCTAAATCAG ATACTTTACC GATGTGAATC AGAAGAACAA 
GAAGATGGTG GAGGGTGTTA TGACATACCA AAATGGTCAT CCCTTAAATA TGCAGGTCTT 
CAAGGGTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT 
TGTGATAATT TAAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA 
CGATCGGGAA CTATTGCTGA AGTTGGTAAA TGGTTCCAGG CTATGTTCTT CTACCTGAAG 
CAGATTCCAC GTTACCTTAT CCCATGTTAC TTCGATGCTA TATTAATTGG TGCATACACC 
ACTCTTCTGG ATATAGCATG GAAGCAGATG TCAAGCTTTG TGCAGAATGG GTCAACGTTT 
GTGAAACACC TTTCGTTGGG TTCAGTTCAG TTGTGTGGAG TAGGAAAATT CCCTTCTCTA 
CCACTTCTTT CACCTTCACT TACGGATGTA CCATACAGAC TAAATGAGAT CACAAAAGAA 
AAGGAGCAAT GTTGTGCTTC TCTAGCTGCA GGCTTACCTC ATTTTTCATC TGGTCTTTTC 
CGCTGCTGGG GAAGGGATAC TTTCATTGCT CTTAGAGGTC TGCTGTTGGT TACTGGACGC 
TATGTAGAGG CCAGGAACAT CATTTTAGCA TTTGCTGGTA CCCTGAGACA TGGTCTCATT 
CCTAATCTTC TTGGTGAAGG AATTTATGCC AGATACAACT GCCGGGATGC TGTGTGGTGG 
TGGCTACAGT GTATTCAGGA TTACTGTAAA ATGGTTCCAA ATGGTACAGA CATTCTCAAG 
TGCCCAGTTT CCAGAATGTA TCCTAAAGAT GATTCTCTTC CTTTGCCTGC TGGCACACTG 
GATCAGCCAT TGTTTGAAGT AATCCAGGAA GCTATGCAAA GACACCTGCA GGGCATAGAG 
TTCCGAGAAA GAAACGCTGG TCCACAGATA GATCGAAACA TGAAGGATGA AGGTTTTAAT 
ATAACTGCAG GGGTTAATGA TGAGACAGGA TTTGTTTATG GAGGCAATCA CTTAAATTGT 
GGTACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC 
ACTCCAAGAG ATGGGTCTGC TGTAGAAATT GTGGGCCTGA GTAAATCTAC TGTGCATTGG 
TTGCTGGAAT TATCCAAAAA AAAAATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT 
GGAAAGGTTG TAAGAGTCTC CTATGATGAA TGGAATAGAA AAATACAAGA CCACTTTGAA 
AAGCTATTTT ATGTTTCTGA AGACCCTTCA GATCCTAATG AAAAGCATCC TAATTTGGTT 
CACAAACGTG GCATATACAA AGATAGTTAC GGAGCTTCAA GTCCTTGGTG TGACTACCAG 
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTAGCCCCTG AGCTCTTTAC TGCAGAAAAA 
GCATGGAAAG CTTTGGAAAT TGCAGAAAAA AAACTGCTTG GTCCCCTTGG CATGAAAACT 
TTGGATCCAG ATGATATGGT TTACTGTGGT GTTTATGACA ATGCCTTAGA CAATGACAAC 
TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG 
TATTTTCTTC GTGCAAAATT ATATTTTTCA AAATTGATGG GTCCAGAGAG TTATGCAAAG 
ACTGTGTTTT TGGTTAAAAA CGTTCTTTCC CGACATTATG TTCATCTTGA AAGATCTCCT 
TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGGCCAAT ACTGTCCTTT CAGCTGTGAA 
ACACAAGCCT GGTCAATCGC TGCTGTTCTT GAGACACTTT ATGACTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023595751.1
CDS	400..4590
Translation	MGPFDEWESRLRVAKESGYNMIHFTPLQTLGVSRSCYSLADQLELNPDFSRPDRKYSWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSRDVAEGKYKEKGLPPLIENDHHLNCFRKIVWEDVFPKIHLWEFFQVDVYKTVEQFRRLLTQEHREITKSDPMQHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFHKRLEELNSEKHQLVNYHQEQAVNCLLGNVFYERLADHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGLRLDNCHSTPLRVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPTIAHALFMDITHDNECPIVHRSAYDALPSSTIVSMASCASGSTKGYDELVPHQISVVSEERLYTKWNPEASPSTTGEVNFQSGIIAARCAVNRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNITVEIREHIQLSESKIVKQAGVTKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLIQFSSHFKAGSFAVDSSDPILKIPFASIASKLTLAELNQILYRCESEEQEDGGGCYDIPKWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGTDILKCPVSRMYPKDDSLPLPAGTLDQPLFEVIQEAMQRHLQGIEFRERNAGPQIDRNMKDEGFNITAGVNDETGFVYGGNHLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVHWLLELSKKKIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_023739983.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023739983.1

ORF Insert Sequence:

ATGGGACCCT TTGATGAATG GGAAAGCAGA CTTAGGGTTG CTAAAGAATC AGGTTACAAC 
ATGATTCATT TTACCCCATT GCAGACTCTT GGAGTATCTA GGTCATGCTA CTCTCTGGCT 
GATCAGTTAG AATTAAATCC TGACTTTTCA AGACCTGATC GAAAATATAG CTGGAATGAT 
GTTGGACAGC TAGTGGAAAA ATTGAAAAAG GAATGGAATA TTCTTTGTAT TACTGATGTT 
GTCTACAATC ATACTGCTGC TAACAGTAAA TGGATCCATG AGCATCCAGA GTGTGCATAT 
AACCTCGTGA ATTCTCCACA CTTGAAACCT GCCTGGCTCT TAGACAGAGC ACTTTGGCAT 
TTATCCCGTG ATGTTGCAGA AGGAAAATAC AAAGAAAAAG GATTACCTCC TCTGATTGAA 
AATGATCATC ATTTGAATTG CTTTCGAAAA ATAGTTTGGG AGGATGTTTT TCCAAAGATT 
CATCTCTGGG AATTTTTCCA AGTAGATGTT TACAAAACAG TTGAGCAATT TAGAAGACTT 
CTTACACAAG AACATAGGGA AATAACGAAG TCTGATCCAA TGCAACATCT TAAGATTATT 
CAAGATCCTG AATACCGACG GCTTGGCTGT ACAGTAGATA TGAACATTGC ACTAGCAACT 
TTCATCCCAC ATGACAAAGG GCCAGCTGCA ATTGATGAGT GCTGTAATTG GTTCCATAAG 
AGACTTGAGG AATTAAATTC AGAGAAGCAT CAACTTGTGA ACTATCATCA GGAACAGGCT 
GTTAATTGCC TTTTGGGAAA TGTTTTTTAT GAACGACTGG CTGACCACGG TCCTAAAGTA 
GGACCTGTCA CTAGAAAATA TCCTTTAGTT ACCAGATATT TTACTTTCCC ATTTGAAGAA 
ATGACCCTTT CTGCAGAAGA ATCTATGATT CATCTTCCAG ATAAAGCTTG TTTTCTGATG 
GCACACAATG GATGGGTAAT GGGAGATGAT CCCCTTCGAA ACTTTGCTGA ACCAGGTTCA 
GACGTATACC TAAGGAGAGA ACTTATATGC TGGGGAGACA GTGTTAAATT ACGCTATGGG 
AATAAACCAG AGGACTGTCC TTATCTCTGG GCACATATGA AAAAATACAC TGAAATAGCT 
GCAACTTACT TCCAGGGACT ACGTCTTGAT AACTGCCACT CAACACCTCT TCGTGTAGCT 
GAGTACATGT TGGATGCTGC TAGGAAATTG CAACCCAATT TATATGTAGT AGCCGAACTG 
TTCACAGGAA GTGAAGACCT GGACAATATC TTTGTTACCA GACTGGGCAT TAGTTCCTTA 
ATAAGAGAGG CAATGAGTGC ATATGATAGT CATGAAGAGG GCAGGTTAGT TTACCGATAT 
GGAGGAGAAC CTGTTGGATC CTTTGTTCAA CCCTGTTTGA GGCCTTTAGT GCCAACTATT 
GCACATGCCC TGTTTATGGA CATTACCCAT GACAATGAAT GTCCTATTGT GCATAGATCA 
GCATATGATG CTCTTCCAAG TTCCACAATT GTTTCTATGG CGTCTTGTGC TAGTGGAAGT 
ACTAAAGGCT ATGATGAATT AGTGCCTCAT CAGATTTCAG TGGTTTCTGA AGAACGGCTT 
TACACTAAGT GGAATCCTGA AGCATCGCCA TCCACCACAG GTGAAGTTAA TTTCCAAAGT 
GGAATTATCG CGGCCCGATG CGCTGTCAAT AGACTTCACC AGGAGCTTGG GGCCAAGGGA 
TTTATTCAGG TGTATGTGGA TCAGGTTGAT GAAGACATAG TGGCAGTAAC AAGACATTCA 
CCTAGTATCC ACCAGTCTGT TGTGGCTGTG TCTAGAACTG CTTTCAGGAA TCCCAAGACT 
TCATTTTACA GCAAGGAAGT GCCTCAAATG TGTATCCCTG GCAAAATTGA AGAAGTAGTT 
CTTGAAGCTA GAACTATTGA GAGAAATACA AAACCTTATA GGAAGGATGA GAATTCAATC 
AATGGAATGC CAAATATCAC AGTAGAAATT AGAGAACATA TTCAGCTTAG TGAAAGTAAA 
ATTGTTAAAC AAGCTGGAGT TACCAAAAAA GGACCCAATG AATATATTCA GGAAATAGAA 
TTTGAAAACT TGTCTCCAGG AAGTATTATT GTATTCAGAG TGAGCCTTGA CCCACATGCA 
CAAGTTGCTG TTGGAATTCT TCGAAATCAT CTGATACAAT TCAGTTCTCA CTTTAAAGCT 
GGGAGCTTTG CTGTGGACAG CTCAGATCCT ATATTAAAAA TACCTTTTGC TTCTATTGCC 
TCTAAATTAA CTTTGGCTGA GCTAAATCAG ATACTTTACC GATGTGAATC AGAAGAACAA 
GAAGATGGTG GAGGGTGTTA TGACATACCA AAATGGTCAT CCCTTAAATA TGCAGGTCTT 
CAAGGGTTAA TGTCTGTATT GGCAGAAATA AGACCAAAGA ATGACTTGGG GCATCCTTTT 
TGTGATAATT TAAGATCTGG AGATTGGATG ATTGACTATG TCAGTAACCG GCTTATTTCA 
CGATCGGGAA CTATTGCTGA AGTTGGTAAA TGGTTCCAGG CTATGTTCTT CTACCTGAAG 
CAGATTCCAC GTTACCTTAT CCCATGTTAC TTCGATGCTA TATTAATTGG TGCATACACC 
ACTCTTCTGG ATATAGCATG GAAGCAGATG TCAAGCTTTG TGCAGAATGG GTCAACGTTT 
GTGAAACACC TTTCGTTGGG TTCAGTTCAG TTGTGTGGAG TAGGAAAATT CCCTTCTCTA 
CCACTTCTTT CACCTTCACT TACGGATGTA CCATACAGAC TAAATGAGAT CACAAAAGAA 
AAGGAGCAAT GTTGTGCTTC TCTAGCTGCA GGCTTACCTC ATTTTTCATC TGGTCTTTTC 
CGCTGCTGGG GAAGGGATAC TTTCATTGCT CTTAGAGGTC TGCTGTTGGT TACTGGACGC 
TATGTAGAGG CCAGGAACAT CATTTTAGCA TTTGCTGGTA CCCTGAGACA TGGTCTCATT 
CCTAATCTTC TTGGTGAAGG AATTTATGCC AGATACAACT GCCGGGATGC TGTGTGGTGG 
TGGCTACAGT GTATTCAGGA TTACTGTAAA ATGGTTCCAA ATGGTACAGA CATTCTCAAG 
TGCCCAGTTT CCAGAATGTA TCCTAAAGAT GATTCTCTTC CTTTGCCTGC TGGCACACTG 
GATCAGCCAT TGTTTGAAGT AATCCAGGAA GCTATGCAAA GACACCTGCA GGGCATAGAG 
TTCCGAGAAA GAAACGCTGG TCCACAGATA GATCGAAACA TGAAGGATGA AGGTTTTAAT 
ATAACTGCAG GGGTTAATGA TGAGACAGGA TTTGTTTATG GAGGCAATCA CTTAAATTGT 
GGTACATGGA TGGATAAAAT GGGAGAAAGT GACAGAGCTA GAAACAGAGG AATCCCAGCC 
ACTCCAAGAG ATGGGTCTGC TGTAGAAATT GTGGGCCTGA GTAAATCTAC TGTGCATTGG 
TTGCTGGAAT TATCCAAAAA AAAAATTTTC CCTTATCATG AAGTCACAGT AAAAAGACAT 
GGAAAGGTTG TAAGAGTCTC CTATGATGAA TGGAATAGAA AAATACAAGA CCACTTTGAA 
AAGCTATTTT ATGTTTCTGA AGACCCTTCA GATCCTAATG AAAAGCATCC TAATTTGGTT 
CACAAACGTG GCATATACAA AGATAGTTAC GGAGCTTCAA GTCCTTGGTG TGACTACCAG 
CTCAGGCCTA ATTTTACCAT AGCAATGGTT GTAGCCCCTG AGCTCTTTAC TGCAGAAAAA 
GCATGGAAAG CTTTGGAAAT TGCAGAAAAA AAACTGCTTG GTCCCCTTGG CATGAAAACT 
TTGGATCCAG ATGATATGGT TTACTGTGGT GTTTATGACA ATGCCTTAGA CAATGACAAC 
TACAATCTTG CTAAAGGTTT CAATTATCAC CAAGGACCTG AGTGGCTGTG GCCTATTGGG 
TATTTTCTTC GTGCAAAATT ATATTTTTCA AAATTGATGG GTCCAGAGAG TTATGCAAAG 
ACTGTGTTTT TGGTTAAAAA CGTTCTTTCC CGACATTATG TTCATCTTGA AAGATCTCCT 
TGGAAAGGAC TTCCAGAACT GACCAATGAG AATGGCCAAT ACTGTCCTTT CAGCTGTGAA 
ACACAAGCCT GGTCAATCGC TGCTGTTCTT GAGACACTTT ATGACTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101343034 ( XM_004385714.3 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004385771.1 Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr183577
Clone ID Related Accession (Same CDS sequence)	XM_004385714.3 , XM_023739979.1 , XM_004385714.2 , XM_023739982.1 , XM_023739981.1
Accession Version	XM_004385714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444080.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004385714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004385771.1
CDS	208..4806
Translation	MEHSKQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLKWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHILPLDCVTLQTFLAKCMGPFDEWESRLRVAKESGYNMIHFTPLQTLGVSRSCYSLADQLELNPDFSRPDRKYSWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSRDVAEGKYKEKGLPPLIENDHHLNCFRKIVWEDVFPKIHLWEFFQVDVYKTVEQFRRLLTQEHREITKSDPMQHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFHKRLEELNSEKHQLVNYHQEQAVNCLLGNVFYERLADHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGLRLDNCHSTPLRVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPTIAHALFMDITHDNECPIVHRSAYDALPSSTIVSMASCASGSTKGYDELVPHQISVVSEERLYTKWNPEASPSTTGEVNFQSGIIAARCAVNRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNITVEIREHIQLSESKIVKQAGVTKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLIQFSSHFKAGSFAVDSSDPILKIPFASIASKLTLAELNQILYRCESEEQEDGGGCYDIPKWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGTDILKCPVSRMYPKDDSLPLPAGTLDQPLFEVIQEAMQRHLQGIEFRERNAGPQIDRNMKDEGFNITAGVNDETGFVYGGNHLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVHWLLELSKKKIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_004385714.3
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004385714.3

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101343034 ( XM_023739979.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023595747.1 Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X2, mRNA.

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CloneID	OTr183577
Clone ID Related Accession (Same CDS sequence)	XM_004385714.3 , XM_023739979.1 , XM_004385714.2 , XM_023739982.1 , XM_023739981.1
Accession Version	XM_023739979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444080.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023595747.1
CDS	156..4754
Translation	MEHSKQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLKWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHILPLDCVTLQTFLAKCMGPFDEWESRLRVAKESGYNMIHFTPLQTLGVSRSCYSLADQLELNPDFSRPDRKYSWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSRDVAEGKYKEKGLPPLIENDHHLNCFRKIVWEDVFPKIHLWEFFQVDVYKTVEQFRRLLTQEHREITKSDPMQHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFHKRLEELNSEKHQLVNYHQEQAVNCLLGNVFYERLADHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGLRLDNCHSTPLRVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPTIAHALFMDITHDNECPIVHRSAYDALPSSTIVSMASCASGSTKGYDELVPHQISVVSEERLYTKWNPEASPSTTGEVNFQSGIIAARCAVNRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNITVEIREHIQLSESKIVKQAGVTKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLIQFSSHFKAGSFAVDSSDPILKIPFASIASKLTLAELNQILYRCESEEQEDGGGCYDIPKWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGTDILKCPVSRMYPKDDSLPLPAGTLDQPLFEVIQEAMQRHLQGIEFRERNAGPQIDRNMKDEGFNITAGVNDETGFVYGGNHLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVHWLLELSKKKIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_023739979.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023739979.1

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101343034 ( XM_004385714.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004385771.1 Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), mRNA.

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CloneID	OTr183577
Clone ID Related Accession (Same CDS sequence)	XM_004385714.3 , XM_023739979.1 , XM_004385714.2 , XM_023739982.1 , XM_023739981.1
Accession Version	XM_004385714.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444080.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004385714.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004385771.1
CDS	121..4719
Translation	MEHSKQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLKWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHILPLDCVTLQTFLAKCMGPFDEWESRLRVAKESGYNMIHFTPLQTLGVSRSCYSLADQLELNPDFSRPDRKYSWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSRDVAEGKYKEKGLPPLIENDHHLNCFRKIVWEDVFPKIHLWEFFQVDVYKTVEQFRRLLTQEHREITKSDPMQHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFHKRLEELNSEKHQLVNYHQEQAVNCLLGNVFYERLADHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGLRLDNCHSTPLRVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPTIAHALFMDITHDNECPIVHRSAYDALPSSTIVSMASCASGSTKGYDELVPHQISVVSEERLYTKWNPEASPSTTGEVNFQSGIIAARCAVNRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNITVEIREHIQLSESKIVKQAGVTKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLIQFSSHFKAGSFAVDSSDPILKIPFASIASKLTLAELNQILYRCESEEQEDGGGCYDIPKWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGTDILKCPVSRMYPKDDSLPLPAGTLDQPLFEVIQEAMQRHLQGIEFRERNAGPQIDRNMKDEGFNITAGVNDETGFVYGGNHLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVHWLLELSKKKIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_004385714.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004385714.2

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101343034 ( XM_023739982.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023595750.1 Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X4, mRNA.

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CloneID	OTr183577
Clone ID Related Accession (Same CDS sequence)	XM_004385714.3 , XM_023739979.1 , XM_004385714.2 , XM_023739982.1 , XM_023739981.1
Accession Version	XM_023739982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444080.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023595750.1
CDS	172..4770
Translation	MEHSKQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLKWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHILPLDCVTLQTFLAKCMGPFDEWESRLRVAKESGYNMIHFTPLQTLGVSRSCYSLADQLELNPDFSRPDRKYSWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSRDVAEGKYKEKGLPPLIENDHHLNCFRKIVWEDVFPKIHLWEFFQVDVYKTVEQFRRLLTQEHREITKSDPMQHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFHKRLEELNSEKHQLVNYHQEQAVNCLLGNVFYERLADHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGLRLDNCHSTPLRVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPTIAHALFMDITHDNECPIVHRSAYDALPSSTIVSMASCASGSTKGYDELVPHQISVVSEERLYTKWNPEASPSTTGEVNFQSGIIAARCAVNRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNITVEIREHIQLSESKIVKQAGVTKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLIQFSSHFKAGSFAVDSSDPILKIPFASIASKLTLAELNQILYRCESEEQEDGGGCYDIPKWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGTDILKCPVSRMYPKDDSLPLPAGTLDQPLFEVIQEAMQRHLQGIEFRERNAGPQIDRNMKDEGFNITAGVNDETGFVYGGNHLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVHWLLELSKKKIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_023739982.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023739982.1

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101343034 ( XM_023739981.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023595749.1 Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X3, mRNA.

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CloneID	OTr183577
Clone ID Related Accession (Same CDS sequence)	XM_004385714.3 , XM_023739979.1 , XM_004385714.2 , XM_023739982.1 , XM_023739981.1
Accession Version	XM_023739981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444080.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023595749.1
CDS	245..4843
Translation	MEHSKQIRILFLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGETFNREKFRSLKWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHILPLDCVTLQTFLAKCMGPFDEWESRLRVAKESGYNMIHFTPLQTLGVSRSCYSLADQLELNPDFSRPDRKYSWNDVGQLVEKLKKEWNILCITDVVYNHTAANSKWIHEHPECAYNLVNSPHLKPAWLLDRALWHLSRDVAEGKYKEKGLPPLIENDHHLNCFRKIVWEDVFPKIHLWEFFQVDVYKTVEQFRRLLTQEHREITKSDPMQHLKIIQDPEYRRLGCTVDMNIALATFIPHDKGPAAIDECCNWFHKRLEELNSEKHQLVNYHQEQAVNCLLGNVFYERLADHGPKVGPVTRKYPLVTRYFTFPFEEMTLSAEESMIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEIAATYFQGLRLDNCHSTPLRVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYDSHEEGRLVYRYGGEPVGSFVQPCLRPLVPTIAHALFMDITHDNECPIVHRSAYDALPSSTIVSMASCASGSTKGYDELVPHQISVVSEERLYTKWNPEASPSTTGEVNFQSGIIAARCAVNRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGMPNITVEIREHIQLSESKIVKQAGVTKKGPNEYIQEIEFENLSPGSIIVFRVSLDPHAQVAVGILRNHLIQFSSHFKAGSFAVDSSDPILKIPFASIASKLTLAELNQILYRCESEEQEDGGGCYDIPKWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSNRLISRSGTIAEVGKWFQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQLCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGLLLVTGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQDYCKMVPNGTDILKCPVSRMYPKDDSLPLPAGTLDQPLFEVIQEAMQRHLQGIEFRERNAGPQIDRNMKDEGFNITAGVNDETGFVYGGNHLNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSTVHWLLELSKKKIFPYHEVTVKRHGKVVRVSYDEWNRKIQDHFEKLFYVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSKLMGPESYAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIAAVLETLYDL

Target ORF information:

RefSeq Version	XM_023739981.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (LOC101343034), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023739981.1

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA TTTCTAAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAA CTACAATTCC GATTAGGCCC AACTTTACAG 
GGAAAAGCAG TTACTGTGTA TACAAATTAC CCATTTCCTG GAGAAACATT CAATCGAGAA 
AAATTCCGCT CCCTGAAATG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTTCAACA ATCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATATTACCCT TGGACTGTGT TACTCTCCAG ACATTTTTAG CTAAGTGTAT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCT AAAGAATCAG GTTACAACAT GATTCATTTT 
ACCCCATTGC AGACTCTTGG AGTATCTAGG TCATGCTACT CTCTGGCTGA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATCGA AAATATAGCT GGAATGATGT TGGACAGCTA 
GTGGAAAAAT TGAAAAAGGA ATGGAATATT CTTTGTATTA CTGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GATCCATGAG CATCCAGAGT GTGCATATAA CCTCGTGAAT 
TCTCCACACT TGAAACCTGC CTGGCTCTTA GACAGAGCAC TTTGGCATTT ATCCCGTGAT 
GTTGCAGAAG GAAAATACAA AGAAAAAGGA TTACCTCCTC TGATTGAAAA TGATCATCAT 
TTGAATTGCT TTCGAAAAAT AGTTTGGGAG GATGTTTTTC CAAAGATTCA TCTCTGGGAA 
TTTTTCCAAG TAGATGTTTA CAAAACAGTT GAGCAATTTA GAAGACTTCT TACACAAGAA 
CATAGGGAAA TAACGAAGTC TGATCCAATG CAACATCTTA AGATTATTCA AGATCCTGAA 
TACCGACGGC TTGGCTGTAC AGTAGATATG AACATTGCAC TAGCAACTTT CATCCCACAT 
GACAAAGGGC CAGCTGCAAT TGATGAGTGC TGTAATTGGT TCCATAAGAG ACTTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTTGTGAAC TATCATCAGG AACAGGCTGT TAATTGCCTT 
TTGGGAAATG TTTTTTATGA ACGACTGGCT GACCACGGTC CTAAAGTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGATATTTT ACTTTCCCAT TTGAAGAAAT GACCCTTTCT 
GCAGAAGAAT CTATGATTCA TCTTCCAGAT AAAGCTTGTT TTCTGATGGC ACACAATGGA 
TGGGTAATGG GAGATGATCC CCTTCGAAAC TTTGCTGAAC CAGGTTCAGA CGTATACCTA 
AGGAGAGAAC TTATATGCTG GGGAGACAGT GTTAAATTAC GCTATGGGAA TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACATATGAAA AAATACACTG AAATAGCTGC AACTTACTTC 
CAGGGACTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC GTGTAGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGACCTGG ACAATATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATGATAGTCA TGAAGAGGGC AGGTTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAACC CTGTTTGAGG CCTTTAGTGC CAACTATTGC ACATGCCCTG 
TTTATGGACA TTACCCATGA CAATGAATGT CCTATTGTGC ATAGATCAGC ATATGATGCT 
CTTCCAAGTT CCACAATTGT TTCTATGGCG TCTTGTGCTA GTGGAAGTAC TAAAGGCTAT 
GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG AACGGCTTTA CACTAAGTGG 
AATCCTGAAG CATCGCCATC CACCACAGGT GAAGTTAATT TCCAAAGTGG AATTATCGCG 
GCCCGATGCG CTGTCAATAG ACTTCACCAG GAGCTTGGGG CCAAGGGATT TATTCAGGTG 
TATGTGGATC AGGTTGATGA AGACATAGTG GCAGTAACAA GACATTCACC TAGTATCCAC 
CAGTCTGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGAAGTGC CTCAAATGTG TATCCCTGGC AAAATTGAAG AAGTAGTTCT TGAAGCTAGA 
ACTATTGAGA GAAATACAAA ACCTTATAGG AAGGATGAGA ATTCAATCAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAGTG AAAGTAAAAT TGTTAAACAA 
GCTGGAGTTA CCAAAAAAGG ACCCAATGAA TATATTCAGG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTATTATTGT ATTCAGAGTG AGCCTTGACC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GATACAATTC AGTTCTCACT TTAAAGCTGG GAGCTTTGCT 
GTGGACAGCT CAGATCCTAT ATTAAAAATA CCTTTTGCTT CTATTGCCTC TAAATTAACT 
TTGGCTGAGC TAAATCAGAT ACTTTACCGA TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGGTGTTATG ACATACCAAA ATGGTCATCC CTTAAATATG CAGGTCTTCA AGGGTTAATG 
TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC ATCCTTTTTG TGATAATTTA 
AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC TTATTTCACG ATCGGGAACT 
ATTGCTGAAG TTGGTAAATG GTTCCAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGT 
TACCTTATCC CATGTTACTT CGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTG CAGAATGGGT CAACGTTTGT GAAACACCTT 
TCGTTGGGTT CAGTTCAGTT GTGTGGAGTA GGAAAATTCC CTTCTCTACC ACTTCTTTCA 
CCTTCACTTA CGGATGTACC ATACAGACTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGCTTCTC TAGCTGCAGG CTTACCTCAT TTTTCATCTG GTCTTTTCCG CTGCTGGGGA 
AGGGATACTT TCATTGCTCT TAGAGGTCTG CTGTTGGTTA CTGGACGCTA TGTAGAGGCC 
AGGAACATCA TTTTAGCATT TGCTGGTACC CTGAGACATG GTCTCATTCC TAATCTTCTT 
GGTGAAGGAA TTTATGCCAG ATACAACTGC CGGGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTACAGACA TTCTCAAGTG CCCAGTTTCC 
AGAATGTATC CTAAAGATGA TTCTCTTCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGAAGTAA TCCAGGAAGC TATGCAAAGA CACCTGCAGG GCATAGAGTT CCGAGAAAGA 
AACGCTGGTC CACAGATAGA TCGAAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTAATGATG AGACAGGATT TGTTTATGGA GGCAATCACT TAAATTGTGG TACATGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TAGAAATTGT GGGCCTGAGT AAATCTACTG TGCATTGGTT GCTGGAATTA 
TCCAAAAAAA AAATTTTCCC TTATCATGAA GTCACAGTAA AAAGACATGG AAAGGTTGTA 
AGAGTCTCCT ATGATGAATG GAATAGAAAA ATACAAGACC ACTTTGAAAA GCTATTTTAT 
GTTTCTGAAG ACCCTTCAGA TCCTAATGAA AAGCATCCTA ATTTGGTTCA CAAACGTGGC 
ATATACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCTAAT 
TTTACCATAG CAATGGTTGT AGCCCCTGAG CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAAATTG CAGAAAAAAA ACTGCTTGGT CCCCTTGGCA TGAAAACTTT GGATCCAGAT 
GATATGGTTT ACTGTGGTGT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTATTGGGTA TTTTCTTCGT 
GCAAAATTAT ATTTTTCAAA ATTGATGGGT CCAGAGAGTT ATGCAAAGAC TGTGTTTTTG 
GTTAAAAACG TTCTTTCCCG ACATTATGTT CATCTTGAAA GATCTCCTTG GAAAGGACTT 
CCAGAACTGA CCAATGAGAA TGGCCAATAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATCGCTG CTGTTCTTGA GACACTTTAT GACTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

LOC101343034 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム