LOC101342232 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101342232
Entrez Gene ID	101342232
Full Name	DLG associated protein 2
Synonyms	DLGAP2
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012554554.1	XP_012410008.1	disks large-associated protein 2
XM_012554554.2	XP_012410008.2	disks large-associated protein 2

Pathways from BioSystems
Gene Ontology

The following LOC101342232 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101342232 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr169468	XM_012554554.1 Latest version!	Trichechus manatus latirostris discs, large (Drosophila) homolog-associated protein 2 (LOC101342232), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTr216885	XM_012554554.2 Latest version!	Trichechus manatus latirostris DLG associated protein 2 (LOC101342232), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101342232 ( XM_012554554.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012410008.1 Trichechus manatus latirostris discs, large (Drosophila) homolog-associated protein 2 (LOC101342232), mRNA.

Price & Availability↑

CloneID	OTr169468
Clone ID Related Accession (Same CDS sequence)	XM_012554554.1
Accession Version	XM_012554554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	disks large-associated protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGAAAGGGT TATCCGGGAG TCGAAATCAA CCACAGTTAT GTTCTGGTCA TACTTGTGGC 
TTAACACCCC CTGAAGATTG TGATCATACT CATGACCACA TTCACCATGG GCAGGAAACA 
AGGCAATCCT ACTTGCTCAG CCCAACTGAC AGCTGCCCCA TGGACCGCCA TCGTTGTTCA 
CCCAGAAGCT CCATCCATTC CGAATGTATG ATGATGCCCA TGATGATGGG CGATCATATG 
TCTAGTAGCA CTTTTCCCAG AATGCACTAT AGCTCGCATT ACGATACAAG AGACGATTGT 
GCCATGTCTC ACGCTAGTAC TAAAATTAAT CGAATTCCAG CCAATCTTTT GGACCAGTTT 
GAGAAACAAC TCCCTCTCCA CAGGGATGGA TTCCATACTT TACAATACCA GAGGACTTCA 
ACCGCTGCAG AACAACGCAG TGAGAGTCCA GGGAGAATAC GACACCTTGT CCATTCTGTC 
CAGAAGCTCT TCACCAAGTC TCATTCTTTA GAAGGCTCTT CCAAGAGTAA CGTCAATGGG 
ACCAAAGGTG ACAGTAGAGT GGATGATCAC CACCACAGCC ACCATTCGAA ACACAGTAAA 
AGAAGTAAAA GCAAGGAGCG GAAACCTGAA AGCAAGCATA AATCTGGCAT GAGTAGTTGG 
TGGAGTTCTG ATGACAATTT GGACAGTGAT AGCACCTACC GAACACCCAG TGTGGTTAAT 
AGGCATCATG TAGATCACAT CTCCCACTGC TACCCTGAAA CTCTCCAGGG CCCCTTTGGG 
GATCTTTCAC TGAAGACTTC CAAAAGTAAT AACGACGTCA AGTGTTCTGC CTGTGAAGGT 
TTATCTATGA CCCCGGATGC AAAGTACATG AAAAGGAGCT CCTGGTCCAC TCTTACAGTA 
AGCCAGGCAA AAGAAGCTTA TCGCAAGAGT TCCCTTAACT TGGATAAGCC TCTGGTCCAT 
CAGGAAATCA AGCCTTCCTT GAGGCCATGC CATTACCTTC AGGTGCCTCA AGATGAGTGG 
GGAGGGTACC CAGCTGGTGG TAAAGATGAA GAAATACCCT GCAGGAGAAT GAGAAGTGGG 
AGTTATATTA AAGCCATGGG GGATGAGGAG AGCGGAGAAT CAGACTCTAG CCCCAAAATG 
TCACCAAAGG TAGCAATCCG ACCGGAGCCC TTGTTAAAGT CCATTGGACA GAGACCACTT 
GGAGATCACC AAACCCCGAG CTACCTGCAA GCTGCAAGTG ACGTGCCTGT TGGTCACAAC 
CTGGATCCCT CTGCCAACTA CAACTCCCCG AAATTCCGCT CCAGGAACCA GAGCTACATG 
AGAGCCGTCA GCACCCTGAG TCAGGCCAGC TGTGTGAGCC AGATGAGTGA AGCTGAGATC 
AATGGGCAGT TTGAATCGGT GTGTGAATCC GTCTTTAGTG AAGTTGAATC TCAGGCCATG 
GATGCCCTTG ACCTCCCTGG ATGTTTCCGA ACAAGGAGTC ACAGTTACCT TCGGGCCATT 
CAAGCTGGTT ACTCCCAAGA CGATGAATGT ATTCCTGCAA TGACATCCTC CAACATCGCC 
TCAACCATCA GGTCAACAGC AGCTGTATCG TACACAAACT ACAAAAAAAC ACCCCCACCG 
GTACCTCCGC GGACCACCTC TAAGCCGCTG ATTTCGGTGA CAGCCCAGAG CAGCACCGAA 
TCCACCCAGG ACGCCTATCA CGACAGCCGG ACTCAGAGGA TTTCCCCGTG GCCGCAGGAT 
GGCCGAGGCC TCTACAACTC CAGCGACAGT TTGGACAGCA ACAAGGCCAT GAATCTTGCT 
CTAGAAACGG CCGCTGCTCA GCGCCATGCC TCGGAGAGCC AGGGCGGCCC AGCACGGACC 
AGCGACAAGG CAATTCTAGT ATCCAAAGCC GAAGAGTTCC TCAAGAGCCA CCGGTCCTCT 
GTGGGGATCC AGGATTCTGA ATTCCCAGAG CATCAGCCAT ACCCAAGGTC AGATGTGGAA 
ACAGCTACAG ATTCTGACAC GGAGAGCCGA GGCCTACGGG AATATCACTC TGTTGGCGTG 
CAAGTAGAAG ATGAAAAGCG ACACGGACGT TTTAAACGTT CCAATAGTGT AACTGCCGCC 
GTCCAAGCAG ACTTGGAACT GGAGGGCTTC CCGGGACACA TCACCATGGA GGACAAAGGT 
CTCCAGTTTG GTTCATCCTT CCAGCGACAC TCAGAACCTA GCACTCCTAC TCAGTATGGA 
GCGGTAAGAA CTGTACGGAC ACAGGGACTC TTCAGTTACA GAGAAGATTA TAGGACCCAA 
GTTGATACTT CAAACCTTCC CCCTCCCGAC CCGTGGTTGG AGCCATCCAT TGAGACAGTA 
GAAACTGGGA GAATGTCTCC ATGTCGCAGA GATGGGTCAT GGTTTTTGAA ACTGCTGCAC 
ACAGAGACAA AGAGAATGGA AGGCTGGTGT AAAGAGATGG AAAGAGAAGC AGAAGAAAAT 
GATCTCTCAG AAGAAACTCA TTATACTTCT CGTTCTGTCA TCAATGGAGA ATGTGGCTCT 
AGTTGCGAGA TGTTTTGTTC CGTTGTTCTT CACGATCAAA GGCTGAATTC AGACCAGATA 
CAAGATCCCA GCGCCATGCC AAGGCCTACC TCCCAGGACC TCGCAGGGTA CTGGGACATG 
TTGCAGCTTT CCATTGAGGA TGTCAGCATG AAGTTTGATG AACTGAACCA GTTAAAGCTC 
CATGACTGGA AGATAATAGA GTCACCTGAG AGGGAGGAAG AGAGAAAGGT CCCCCCTCCA 
ATACCAAAGA AGCCCCCAAA AGGGAAATTT CCTATCACGA GAGAAAAGTC CCTTGACCTG 
CCCGACAGAC AACGTCAAGA AGCTCGAAGA CGCCTCATGG CCGCTAAGAG AGCAGCGTCC 
TTCCGACAGA ATTCTGCCAC CGAGCGGGCT GACAGCATTG AGATCTACAT CCCGGAGGCC 
CAGACCCGAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012410008.1
CDS	61..3015
Translation	MKGLSGSRNQPQLCSGHTCGLTPPEDCDHTHDHIHHGQETRQSYLLSPTDSCPMDRHRCSPRSSIHSECMMMPMMMGDHMSSSTFPRMHYSSHYDTRDDCAMSHASTKINRIPANLLDQFEKQLPLHRDGFHTLQYQRTSTAAEQRSESPGRIRHLVHSVQKLFTKSHSLEGSSKSNVNGTKGDSRVDDHHHSHHSKHSKRSKSKERKPESKHKSGMSSWWSSDDNLDSDSTYRTPSVVNRHHVDHISHCYPETLQGPFGDLSLKTSKSNNDVKCSACEGLSMTPDAKYMKRSSWSTLTVSQAKEAYRKSSLNLDKPLVHQEIKPSLRPCHYLQVPQDEWGGYPAGGKDEEIPCRRMRSGSYIKAMGDEESGESDSSPKMSPKVAIRPEPLLKSIGQRPLGDHQTPSYLQAASDVPVGHNLDPSANYNSPKFRSRNQSYMRAVSTLSQASCVSQMSEAEINGQFESVCESVFSEVESQAMDALDLPGCFRTRSHSYLRAIQAGYSQDDECIPAMTSSNIASTIRSTAAVSYTNYKKTPPPVPPRTTSKPLISVTAQSSTESTQDAYHDSRTQRISPWPQDGRGLYNSSDSLDSNKAMNLALETAAAQRHASESQGGPARTSDKAILVSKAEEFLKSHRSSVGIQDSEFPEHQPYPRSDVETATDSDTESRGLREYHSVGVQVEDEKRHGRFKRSNSVTAAVQADLELEGFPGHITMEDKGLQFGSSFQRHSEPSTPTQYGAVRTVRTQGLFSYREDYRTQVDTSNLPPPDPWLEPSIETVETGRMSPCRRDGSWFLKLLHTETKRMEGWCKEMEREAEENDLSEETHYTSRSVINGECGSSCEMFCSVVLHDQRLNSDQIQDPSAMPRPTSQDLAGYWDMLQLSIEDVSMKFDELNQLKLHDWKIIESPEREEERKVPPPIPKKPPKGKFPITREKSLDLPDRQRQEARRRLMAAKRAASFRQNSATERADSIEIYIPEAQTRL

Target ORF information:

RefSeq Version	XM_012554554.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris discs, large (Drosophila) homolog-associated protein 2 (LOC101342232), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554554.1

ORF Insert Sequence:

ATGAAAGGGT TATCCGGGAG TCGAAATCAA CCACAGTTAT GTTCTGGTCA TACTTGTGGC 
TTAACACCCC CTGAAGATTG TGATCATACT CATGACCACA TTCACCATGG GCAGGAAACA 
AGGCAATCCT ACTTGCTCAG CCCAACTGAC AGCTGCCCCA TGGACCGCCA TCGTTGTTCA 
CCCAGAAGCT CCATCCATTC CGAATGTATG ATGATGCCCA TGATGATGGG CGATCATATG 
TCTAGTAGCA CTTTTCCCAG AATGCACTAT AGCTCGCATT ACGATACAAG AGACGATTGT 
GCCATGTCTC ACGCTAGTAC TAAAATTAAT CGAATTCCAG CCAATCTTTT GGACCAGTTT 
GAGAAACAAC TCCCTCTCCA CAGGGATGGA TTCCATACTT TACAATACCA GAGGACTTCA 
ACCGCTGCAG AACAACGCAG TGAGAGTCCA GGGAGAATAC GACACCTTGT CCATTCTGTC 
CAGAAGCTCT TCACCAAGTC TCATTCTTTA GAAGGCTCTT CCAAGAGTAA CGTCAATGGG 
ACCAAAGGTG ACAGTAGAGT GGATGATCAC CACCACAGCC ACCATTCGAA ACACAGTAAA 
AGAAGTAAAA GCAAGGAGCG GAAACCTGAA AGCAAGCATA AATCTGGCAT GAGTAGTTGG 
TGGAGTTCTG ATGACAATTT GGACAGTGAT AGCACCTACC GAACACCCAG TGTGGTTAAT 
AGGCATCATG TAGATCACAT CTCCCACTGC TACCCTGAAA CTCTCCAGGG CCCCTTTGGG 
GATCTTTCAC TGAAGACTTC CAAAAGTAAT AACGACGTCA AGTGTTCTGC CTGTGAAGGT 
TTATCTATGA CCCCGGATGC AAAGTACATG AAAAGGAGCT CCTGGTCCAC TCTTACAGTA 
AGCCAGGCAA AAGAAGCTTA TCGCAAGAGT TCCCTTAACT TGGATAAGCC TCTGGTCCAT 
CAGGAAATCA AGCCTTCCTT GAGGCCATGC CATTACCTTC AGGTGCCTCA AGATGAGTGG 
GGAGGGTACC CAGCTGGTGG TAAAGATGAA GAAATACCCT GCAGGAGAAT GAGAAGTGGG 
AGTTATATTA AAGCCATGGG GGATGAGGAG AGCGGAGAAT CAGACTCTAG CCCCAAAATG 
TCACCAAAGG TAGCAATCCG ACCGGAGCCC TTGTTAAAGT CCATTGGACA GAGACCACTT 
GGAGATCACC AAACCCCGAG CTACCTGCAA GCTGCAAGTG ACGTGCCTGT TGGTCACAAC 
CTGGATCCCT CTGCCAACTA CAACTCCCCG AAATTCCGCT CCAGGAACCA GAGCTACATG 
AGAGCCGTCA GCACCCTGAG TCAGGCCAGC TGTGTGAGCC AGATGAGTGA AGCTGAGATC 
AATGGGCAGT TTGAATCGGT GTGTGAATCC GTCTTTAGTG AAGTTGAATC TCAGGCCATG 
GATGCCCTTG ACCTCCCTGG ATGTTTCCGA ACAAGGAGTC ACAGTTACCT TCGGGCCATT 
CAAGCTGGTT ACTCCCAAGA CGATGAATGT ATTCCTGCAA TGACATCCTC CAACATCGCC 
TCAACCATCA GGTCAACAGC AGCTGTATCG TACACAAACT ACAAAAAAAC ACCCCCACCG 
GTACCTCCGC GGACCACCTC TAAGCCGCTG ATTTCGGTGA CAGCCCAGAG CAGCACCGAA 
TCCACCCAGG ACGCCTATCA CGACAGCCGG ACTCAGAGGA TTTCCCCGTG GCCGCAGGAT 
GGCCGAGGCC TCTACAACTC CAGCGACAGT TTGGACAGCA ACAAGGCCAT GAATCTTGCT 
CTAGAAACGG CCGCTGCTCA GCGCCATGCC TCGGAGAGCC AGGGCGGCCC AGCACGGACC 
AGCGACAAGG CAATTCTAGT ATCCAAAGCC GAAGAGTTCC TCAAGAGCCA CCGGTCCTCT 
GTGGGGATCC AGGATTCTGA ATTCCCAGAG CATCAGCCAT ACCCAAGGTC AGATGTGGAA 
ACAGCTACAG ATTCTGACAC GGAGAGCCGA GGCCTACGGG AATATCACTC TGTTGGCGTG 
CAAGTAGAAG ATGAAAAGCG ACACGGACGT TTTAAACGTT CCAATAGTGT AACTGCCGCC 
GTCCAAGCAG ACTTGGAACT GGAGGGCTTC CCGGGACACA TCACCATGGA GGACAAAGGT 
CTCCAGTTTG GTTCATCCTT CCAGCGACAC TCAGAACCTA GCACTCCTAC TCAGTATGGA 
GCGGTAAGAA CTGTACGGAC ACAGGGACTC TTCAGTTACA GAGAAGATTA TAGGACCCAA 
GTTGATACTT CAAACCTTCC CCCTCCCGAC CCGTGGTTGG AGCCATCCAT TGAGACAGTA 
GAAACTGGGA GAATGTCTCC ATGTCGCAGA GATGGGTCAT GGTTTTTGAA ACTGCTGCAC 
ACAGAGACAA AGAGAATGGA AGGCTGGTGT AAAGAGATGG AAAGAGAAGC AGAAGAAAAT 
GATCTCTCAG AAGAAACTCA TTATACTTCT CGTTCTGTCA TCAATGGAGA ATGTGGCTCT 
AGTTGCGAGA TGTTTTGTTC CGTTGTTCTT CACGATCAAA GGCTGAATTC AGACCAGATA 
CAAGATCCCA GCGCCATGCC AAGGCCTACC TCCCAGGACC TCGCAGGGTA CTGGGACATG 
TTGCAGCTTT CCATTGAGGA TGTCAGCATG AAGTTTGATG AACTGAACCA GTTAAAGCTC 
CATGACTGGA AGATAATAGA GTCACCTGAG AGGGAGGAAG AGAGAAAGGT CCCCCCTCCA 
ATACCAAAGA AGCCCCCAAA AGGGAAATTT CCTATCACGA GAGAAAAGTC CCTTGACCTG 
CCCGACAGAC AACGTCAAGA AGCTCGAAGA CGCCTCATGG CCGCTAAGAG AGCAGCGTCC 
TTCCGACAGA ATTCTGCCAC CGAGCGGGCT GACAGCATTG AGATCTACAT CCCGGAGGCC 
CAGACCCGAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101342232 ( XM_012554554.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012410008.2 Trichechus manatus latirostris DLG associated protein 2 (LOC101342232), mRNA.

Price & Availability↑

CloneID	OTr216885
Clone ID Related Accession (Same CDS sequence)	XM_012554554.2
Accession Version	XM_012554554.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	disks large-associated protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_012554554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGTGAAGA ATTTGGATGA CTGCCGCCTA CACCAGGGAA AGGTACTAAA GAGTCACTTT 
GTTTCTGTCT TTACAGAGGA AGAGGGTGGA GATCTGGCCC AGCCGGGCAT CAGTTTTCCA 
GGGTCCACAG AGGAGGAACT AGACCAGCAG TACTCATGGT CACCGACTCA GCACTTCAAT 
GAAGAAAGAT ACTCCCCTGC TCCTAGGAAT ATGAAAGGGT TATCCGGGAG TCGAAATCAA 
CCACAGTTAT GTTCTGGTCA TACTTGTGGC TTAACACCCC CTGAAGATTG TGATCATACT 
CATGACCACA TTCACCATGG GCAGGAAACA AGGCAATCCT ACTTGCTCAG CCCAACTGAC 
AGCTGCCCCA TGGACCGCCA TCGTTGTTCA CCCAGAAGCT CCATCCATTC CGAATGTATG 
ATGATGCCCA TGATGATGGG CGATCATATG TCTAGTAGCA CTTTTCCCAG AATGCACTAT 
AGCTCGCATT ACGATACAAG AGACGATTGT GCCATGTCTC ACGCTAGTAC TAAAATTAAT 
CGAATTCCAG CCAATCTTTT GGACCAGTTT GAGAAACAAC TCCCTCTCCA CAGGGATGGA 
TTCCATACTT TACAATACCA GAGGACTTCA ACCGCTGCAG AACAACGCAG TGAGAGTCCA 
GGGAGAATAC GACACCTTGT CCATTCTGTC CAGAAGCTCT TCACCAAGTC TCATTCTTTA 
GAAGGCTCTT CCAAGAGTAA CGTCAATGGG ACCAAAGGTG ACAGTAGAGT GGATGATCAC 
CACCACAGCC ACCATTCGAA ACACAGTAAA AGAAGTAAAA GCAAGGAGCG GAAACCTGAA 
AGCAAGCATA AATCTGGCAT GAGTAGTTGG TGGAGTTCTG ATGACAATTT GGACAGTGAT 
AGCACCTACC GAACACCCAG TGTGGTTAAT AGGCATCATG TAGATCACAT CTCCCACTGC 
TACCCTGAAA CTCTCCAGGG CCCCTTTGGG GATCTTTCAC TGAAGACTTC CAAAAGTAAT 
AACGACGTCA AGTGTTCTGC CTGTGAAGGT TTATCTATGA CCCCGGATGC AAAGTACATG 
AAAAGGAGCT CCTGGTCCAC TCTTACAGTA AGCCAGGCAA AAGAAGCTTA TCGCAAGAGT 
TCCCTTAACT TGGATAAGCC TCTGGTCCAT CAGGAAATCA AGCCTTCCTT GAGGCCATGC 
CATTACCTTC AGGTGCCTCA AGATGAGTGG GGAGGGTACC CAGCTGGTGG TAAAGATGAA 
GAAATACCCT GCAGGAGAAT GAGAAGTGGG AGTTATATTA AAGCCATGGG GGATGAGGAG 
AGCGGAGAAT CAGACTCTAG CCCCAAAATG TCACCAAAGG TAGCAATCCG ACCGGAGCCC 
TTGTTAAAGT CCATTGGACA GAGACCACTT GGAGATCACC AAACCCCGAG CTACCTGCAA 
GCTGCAAGTG ACGTGCCTGT TGGTCACAAC CTGGATCCCT CTGCCAACTA CAACTCCCCG 
AAATTCCGCT CCAGGAACCA GAGCTACATG AGAGCCGTCA GCACCCTGAG TCAGGCCAGC 
TGTGTGAGCC AGATGAGTGA AGCTGAGATC AATGGGCAGT TTGAATCGGT GTGTGAATCC 
GTCTTTAGTG AAGTTGAATC TCAGGCCATG GATGCCCTTG ACCTCCCTGG ATGTTTCCGA 
ACAAGGAGTC ACAGTTACCT TCGGGCCATT CAAGCTGGTT ACTCCCAAGA CGATGAATGT 
ATTCCTGCAA TGACATCCTC CAACATCGCC TCAACCATCA GGTCAACAGC AGCTGTATCG 
TACACAAACT ACAAAAAAAC ACCCCCACCG GTACCTCCGC GGACCACCTC TAAGCCGCTG 
ATTTCGGTGA CAGCCCAGAG CAGCACCGAA TCCACCCAGG ACGCCTATCA CGACAGCCGG 
ACTCAGAGGA TTTCCCCGTG GCCGCAGGAT GGCCGAGGCC TCTACAACTC CAGCGACAGT 
TTGGACAGCA ACAAGGCCAT GAATCTTGCT CTAGAAACGG CCGCTGCTCA GCGCCATGCC 
TCGGAGAGCC AGGGCGGCCC AGCACGGACC AGCGACAAGG CAATTCTAGT ATCCAAAGCC 
GAAGAGTTCC TCAAGAGCCA CCGGTCCTCT GTGGGGATCC AGGATTCTGA ATTCCCAGAG 
CATCAGCCAT ACCCAAGGTC AGATGTGGAA ACAGCTACAG ATTCTGACAC GGAGAGCCGA 
GGCCTACGGG AATATCACTC TGTTGGCGTG CAAGTAGAAG ATGAAAAGCG ACACGGACGT 
TTTAAACGTT CCAATAGTGT AACTGCCGCC GTCCAAGCAG ACTTGGAACT GGAGGGCTTC 
CCGGGACACA TCACCATGGA GGACAAAGGT CTCCAGTTTG GTTCATCCTT CCAGCGACAC 
TCAGAACCTA GCACTCCTAC TCAGTATGGA GCGGTAAGAA CTGTACGGAC ACAGGGACTC 
TTCAGTTACA GAGAAGATTA TAGGACCCAA GTTGATACTT CAAACCTTCC CCCTCCCGAC 
CCGTGGTTGG AGCCATCCAT TGAGACAGTA GAAACTGGGA GAATGTCTCC ATGTCGCAGA 
GATGGGTCAT GGTTTTTGAA ACTGCTGCAC ACAGAGACAA AGAGAATGGA AGGCTGGTGT 
AAAGAGATGG AAAGAGAAGC AGAAGAAAAT GATCTCTCAG AAGAAACTCA TTATACTTCT 
CGTTCTGTCA TCAATGGAGA ATGTGGCTCT AGTTGCGAGA TGTTTTGTTC CGTTGTTCTT 
CACGATCAAA GGCTGAATTC AGACCAGATA CAAGATCCCA GCGCCATGCC AAGGCCTACC 
TCCCAGGACC TCGCAGGGTA CTGGGACATG TTGCAGCTTT CCATTGAGGA TGTCAGCATG 
AAGTTTGATG AACTGAACCA GTTAAAGCTC CATGACTGGA AGATAATAGA GTCACCTGAG 
AGGGAGGAAG AGAGAAAGGT CCCCCCTCCA ATACCAAAGA AGCCCCCAAA AGGGAAATTT 
CCTATCACGA GAGAAAAGTC CCTTGACCTG CCCGACAGAC AACGTCAAGA AGCTCGAAGA 
CGCCTCATGG CCGCTAAGAG AGCAGCGTCC TTCCGACAGA ATTCTGCCAC CGAGCGGGCT 
GACAGCATTG AGATCTACAT CCCGGAGGCC CAGACCCGAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012410008.2
CDS	382..3546
Translation	MVKNLDDCRLHQGKVLKSHFVSVFTEEEGGDLAQPGISFPGSTEEELDQQYSWSPTQHFNEERYSPAPRNMKGLSGSRNQPQLCSGHTCGLTPPEDCDHTHDHIHHGQETRQSYLLSPTDSCPMDRHRCSPRSSIHSECMMMPMMMGDHMSSSTFPRMHYSSHYDTRDDCAMSHASTKINRIPANLLDQFEKQLPLHRDGFHTLQYQRTSTAAEQRSESPGRIRHLVHSVQKLFTKSHSLEGSSKSNVNGTKGDSRVDDHHHSHHSKHSKRSKSKERKPESKHKSGMSSWWSSDDNLDSDSTYRTPSVVNRHHVDHISHCYPETLQGPFGDLSLKTSKSNNDVKCSACEGLSMTPDAKYMKRSSWSTLTVSQAKEAYRKSSLNLDKPLVHQEIKPSLRPCHYLQVPQDEWGGYPAGGKDEEIPCRRMRSGSYIKAMGDEESGESDSSPKMSPKVAIRPEPLLKSIGQRPLGDHQTPSYLQAASDVPVGHNLDPSANYNSPKFRSRNQSYMRAVSTLSQASCVSQMSEAEINGQFESVCESVFSEVESQAMDALDLPGCFRTRSHSYLRAIQAGYSQDDECIPAMTSSNIASTIRSTAAVSYTNYKKTPPPVPPRTTSKPLISVTAQSSTESTQDAYHDSRTQRISPWPQDGRGLYNSSDSLDSNKAMNLALETAAAQRHASESQGGPARTSDKAILVSKAEEFLKSHRSSVGIQDSEFPEHQPYPRSDVETATDSDTESRGLREYHSVGVQVEDEKRHGRFKRSNSVTAAVQADLELEGFPGHITMEDKGLQFGSSFQRHSEPSTPTQYGAVRTVRTQGLFSYREDYRTQVDTSNLPPPDPWLEPSIETVETGRMSPCRRDGSWFLKLLHTETKRMEGWCKEMEREAEENDLSEETHYTSRSVINGECGSSCEMFCSVVLHDQRLNSDQIQDPSAMPRPTSQDLAGYWDMLQLSIEDVSMKFDELNQLKLHDWKIIESPEREEERKVPPPIPKKPPKGKFPITREKSLDLPDRQRQEARRRLMAAKRAASFRQNSATERADSIEIYIPEAQTRL

Target ORF information:

RefSeq Version	XM_012554554.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris DLG associated protein 2 (LOC101342232), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554554.2

ORF Insert Sequence:

ATGGTGAAGA ATTTGGATGA CTGCCGCCTA CACCAGGGAA AGGTACTAAA GAGTCACTTT 
GTTTCTGTCT TTACAGAGGA AGAGGGTGGA GATCTGGCCC AGCCGGGCAT CAGTTTTCCA 
GGGTCCACAG AGGAGGAACT AGACCAGCAG TACTCATGGT CACCGACTCA GCACTTCAAT 
GAAGAAAGAT ACTCCCCTGC TCCTAGGAAT ATGAAAGGGT TATCCGGGAG TCGAAATCAA 
CCACAGTTAT GTTCTGGTCA TACTTGTGGC TTAACACCCC CTGAAGATTG TGATCATACT 
CATGACCACA TTCACCATGG GCAGGAAACA AGGCAATCCT ACTTGCTCAG CCCAACTGAC 
AGCTGCCCCA TGGACCGCCA TCGTTGTTCA CCCAGAAGCT CCATCCATTC CGAATGTATG 
ATGATGCCCA TGATGATGGG CGATCATATG TCTAGTAGCA CTTTTCCCAG AATGCACTAT 
AGCTCGCATT ACGATACAAG AGACGATTGT GCCATGTCTC ACGCTAGTAC TAAAATTAAT 
CGAATTCCAG CCAATCTTTT GGACCAGTTT GAGAAACAAC TCCCTCTCCA CAGGGATGGA 
TTCCATACTT TACAATACCA GAGGACTTCA ACCGCTGCAG AACAACGCAG TGAGAGTCCA 
GGGAGAATAC GACACCTTGT CCATTCTGTC CAGAAGCTCT TCACCAAGTC TCATTCTTTA 
GAAGGCTCTT CCAAGAGTAA CGTCAATGGG ACCAAAGGTG ACAGTAGAGT GGATGATCAC 
CACCACAGCC ACCATTCGAA ACACAGTAAA AGAAGTAAAA GCAAGGAGCG GAAACCTGAA 
AGCAAGCATA AATCTGGCAT GAGTAGTTGG TGGAGTTCTG ATGACAATTT GGACAGTGAT 
AGCACCTACC GAACACCCAG TGTGGTTAAT AGGCATCATG TAGATCACAT CTCCCACTGC 
TACCCTGAAA CTCTCCAGGG CCCCTTTGGG GATCTTTCAC TGAAGACTTC CAAAAGTAAT 
AACGACGTCA AGTGTTCTGC CTGTGAAGGT TTATCTATGA CCCCGGATGC AAAGTACATG 
AAAAGGAGCT CCTGGTCCAC TCTTACAGTA AGCCAGGCAA AAGAAGCTTA TCGCAAGAGT 
TCCCTTAACT TGGATAAGCC TCTGGTCCAT CAGGAAATCA AGCCTTCCTT GAGGCCATGC 
CATTACCTTC AGGTGCCTCA AGATGAGTGG GGAGGGTACC CAGCTGGTGG TAAAGATGAA 
GAAATACCCT GCAGGAGAAT GAGAAGTGGG AGTTATATTA AAGCCATGGG GGATGAGGAG 
AGCGGAGAAT CAGACTCTAG CCCCAAAATG TCACCAAAGG TAGCAATCCG ACCGGAGCCC 
TTGTTAAAGT CCATTGGACA GAGACCACTT GGAGATCACC AAACCCCGAG CTACCTGCAA 
GCTGCAAGTG ACGTGCCTGT TGGTCACAAC CTGGATCCCT CTGCCAACTA CAACTCCCCG 
AAATTCCGCT CCAGGAACCA GAGCTACATG AGAGCCGTCA GCACCCTGAG TCAGGCCAGC 
TGTGTGAGCC AGATGAGTGA AGCTGAGATC AATGGGCAGT TTGAATCGGT GTGTGAATCC 
GTCTTTAGTG AAGTTGAATC TCAGGCCATG GATGCCCTTG ACCTCCCTGG ATGTTTCCGA 
ACAAGGAGTC ACAGTTACCT TCGGGCCATT CAAGCTGGTT ACTCCCAAGA CGATGAATGT 
ATTCCTGCAA TGACATCCTC CAACATCGCC TCAACCATCA GGTCAACAGC AGCTGTATCG 
TACACAAACT ACAAAAAAAC ACCCCCACCG GTACCTCCGC GGACCACCTC TAAGCCGCTG 
ATTTCGGTGA CAGCCCAGAG CAGCACCGAA TCCACCCAGG ACGCCTATCA CGACAGCCGG 
ACTCAGAGGA TTTCCCCGTG GCCGCAGGAT GGCCGAGGCC TCTACAACTC CAGCGACAGT 
TTGGACAGCA ACAAGGCCAT GAATCTTGCT CTAGAAACGG CCGCTGCTCA GCGCCATGCC 
TCGGAGAGCC AGGGCGGCCC AGCACGGACC AGCGACAAGG CAATTCTAGT ATCCAAAGCC 
GAAGAGTTCC TCAAGAGCCA CCGGTCCTCT GTGGGGATCC AGGATTCTGA ATTCCCAGAG 
CATCAGCCAT ACCCAAGGTC AGATGTGGAA ACAGCTACAG ATTCTGACAC GGAGAGCCGA 
GGCCTACGGG AATATCACTC TGTTGGCGTG CAAGTAGAAG ATGAAAAGCG ACACGGACGT 
TTTAAACGTT CCAATAGTGT AACTGCCGCC GTCCAAGCAG ACTTGGAACT GGAGGGCTTC 
CCGGGACACA TCACCATGGA GGACAAAGGT CTCCAGTTTG GTTCATCCTT CCAGCGACAC 
TCAGAACCTA GCACTCCTAC TCAGTATGGA GCGGTAAGAA CTGTACGGAC ACAGGGACTC 
TTCAGTTACA GAGAAGATTA TAGGACCCAA GTTGATACTT CAAACCTTCC CCCTCCCGAC 
CCGTGGTTGG AGCCATCCAT TGAGACAGTA GAAACTGGGA GAATGTCTCC ATGTCGCAGA 
GATGGGTCAT GGTTTTTGAA ACTGCTGCAC ACAGAGACAA AGAGAATGGA AGGCTGGTGT 
AAAGAGATGG AAAGAGAAGC AGAAGAAAAT GATCTCTCAG AAGAAACTCA TTATACTTCT 
CGTTCTGTCA TCAATGGAGA ATGTGGCTCT AGTTGCGAGA TGTTTTGTTC CGTTGTTCTT 
CACGATCAAA GGCTGAATTC AGACCAGATA CAAGATCCCA GCGCCATGCC AAGGCCTACC 
TCCCAGGACC TCGCAGGGTA CTGGGACATG TTGCAGCTTT CCATTGAGGA TGTCAGCATG 
AAGTTTGATG AACTGAACCA GTTAAAGCTC CATGACTGGA AGATAATAGA GTCACCTGAG 
AGGGAGGAAG AGAGAAAGGT CCCCCCTCCA ATACCAAAGA AGCCCCCAAA AGGGAAATTT 
CCTATCACGA GAGAAAAGTC CCTTGACCTG CCCGACAGAC AACGTCAAGA AGCTCGAAGA 
CGCCTCATGG CCGCTAAGAG AGCAGCGTCC TTCCGACAGA ATTCTGCCAC CGAGCGGGCT 
GACAGCATTG AGATCTACAT CCCGGAGGCC CAGACCCGAC TTTAA